Publications

variability and interactions between endophytic bacteria and fungi isolated from leaf tissues of citrus rootstocks.	fungi and bacteria were isolated from surface disinfected leaf tissues of several citrus rootstocks. the principal bacterial species isolated were alcaligenes sp., bacillus spp. (including b. cereus, b. lentus, b. megaterium, b. pumilus, and b. subtilis), burkholderia cepacia, curtobacterium flaccumfaciens, enterobacter cloacae, methylobacterium extorquens, and pantoea agglomerans, with p. agglomerans and b. pumilus being the most frequently isolated species. the most abundant fungal species wer ...	2001	11315114
nonpathogenic isolates of the citrus black spot fungus, guignardia citricarpa, identified as a cosmopolitan endophyte of woody plants, g. mangiferae (phyllosticta capitalensis).	abstract the population structure of guignardia citricarpa sensu lato (anamorph: phyllosticta citricarpa), a fungus of which strains pathogenic to citrus are subject to phytosanitary legislation in the european union and the united states, was investigated. internal transcribed spacer sequences revealed two phylogenetically distinct groups in g. citricarpa. this distinction was supported by amplified fragment length polymorphism analysis that also supported the exclusion of two isolates that had ...	2002	18943020
taxol from phyllosticta citricarpa, a leaf spot fungus of the angiosperm citrus medica.	phyllosticta citricarpa, a leaf spot fungus isolated from the diseased leaves of citrus medica, displayed the production of taxol, an anticancer drug on m1d and potato dextrose broth medium in culture for the first time. the presence of taxol was confirmed by spectroscopic and chromatographic methods of analysis. the maximum amount of taxol production was recorded in the fungus grown on m1d medium (265 microg/l) followed by pdb medium (137 microg/l). the production rate was increased to 5.3 x 10 ...	2008	18691540
fungicide resistance and genetic variability in plant pathogenic strains of guignardia citricarpa.	citrus black spot (cbs) is a plant disease of worldwide occurrence, affecting crops in africa, oceania, and south america. in brazil, climate provides favorable conditions and cbs has spread to the southeast and south regions. cbs is caused by the fungus guignardia citricarpa (anamorph: phyllosticta citricarpa) and its control is based on the use of fungicides, such as benzimidazoles. in south africa, the disease was kept under control for 10 years with benomyl, until cases of resistance to high ...	2009	24031363
agrobacterium tumefaciens-mediated transformation of guignardia citricarpa.	guignardia citricarpa, the causal agent of citrus black spot, was successfully transformed via agrobacterium tumefaciens with cassettes for gfp and bar expression. transformation is essential to understand the role of genes during interaction between plants and its pathogens. using a binary plasmid vector based in the ppzp201bk, both germinated conidia and physically fragmented hyphae of g. citricarpa were transformed. eight independent transformants of g. citricarpa resistant to ammonium glifos ...	2010	19995579
Endophytic and pathogenic Phyllosticta species, with reference to those associated with Citrus Black Spot.	We investigated the identity and genetic diversity of more than 100 isolates belonging to Phyllosticta (teleomorph Guignardia), with particular emphasis on Phyllosticta citricarpa and Guignardia mangiferae s.l. occurring on Citrus. Phyllosticta citricarpa is the causal agent of Citrus Black Spot and is subject to phytosanitary legislation in the EU. This species is frequently confused with a taxon generally referred to as G. mangiferae, the presumed teleomorph of P. capitalensis, which is a non- ...	2011	22025803
genetic diversity and population differentiation of the causal agent of citrus black spot in brazil.	one of the most important diseases that affect sweet orange orchards in brazil is the citrus black spot that is caused by the fungus guignardia citricarpa. this disease causes irreparable losses due to the premature falling of fruit, as well as its severe effects on the epidermis of ripe fruit that renders them unacceptable at the fresh fruit markets. despite the fact that the fungus and the disease are well studied, little is known about the genetic diversity and the structure of the fungi popu ...	2012	22666111
modeling the effect of temperature and wetness on guignardia pseudothecium maturation and ascospore release in citrus orchards.	ascospores are the most important inoculum source of citrus black spot (cbs), caused by guignardia citricarpa, but pseudothecium maturation and ascospore release are inadequately studied. guignardia ascospore trapping and concomitant weather data were obtained for three localities over three seasons (july to march 2006 to 2009) in the limpopo province of south africa. degree-days accumulated until first seasonal ascospore discharge (>10°c with 1 july as biofix) (ddtemp), and ddtemp accumulated o ...	2013	23234366
production of 3-nitropropionic acid by endophytic fungus phomopsis longicolla isolated from trichilia elegans a. juss ssp. elegans and evaluation of biological activity.	the compound 3-nitropropionic acid is a potent neurotoxic agent in animals and well-known as a potent inhibitor of mycobacterium tuberculosis. in this research, we were able to extract this compound from the endophytic fungus, phomopsis longicolla (fj62759), isolated from trichilia elegans a. juss ssp. elegans. the aim of this study was the isolation of secondary metabolites produced by p. longicolla, the chemical identification of these compounds and evaluation of their antimicrobial and insect ...	2013	23296917
agrobacterium-mediated transformation of guignardia citricarpa: an efficient tool to gene transfer and random mutagenesis.	guignardia citricarpa is the causal agent of citrus black spot (cbs), an important disease in citriculture. due to the expressive value of this activity worldwide, especially in brazil, understanding more about the functioning of this fungus is of utmost relevance, making possible the elucidation of its infection mechanisms, and providing tools to control cbs. this work describes for the first time an efficient and successful methodology for genetic transformation of g. citricarpa mycelia, which ...	2013	23931121
splash dispersal of phyllosticta citricarpa conidia from infected citrus fruit.	rain-splash dispersal of phyllosticta citricarpa (syn. guignardia citricarpa) conidia (pycnidiospores) from infected oranges was studied in still air and combined with wind. high power microscopy demonstrated the presence of conidia in splash droplets from diseased oranges, which exuded conidia for over one hour during repeated wetting. the largest (5 mm) incident drops produced the highest splashes (up to 41.0 cm). a linear-by-quadratic surface model predicted highest splashes to be 41.91 cm at ...	2014	25298272
antifungal and proteolytic activities of endophytic fungi isolated from piper hispidum sw.	endophytes are being considered for use in biological control, and the enzymes they secrete might facilitate their initial colonization of internal plant tissues and direct interactions with microbial pathogens. microbial proteases are also biotechnologically important products employed in bioremediation processes, cosmetics, and the pharmaceutical, photographic and food industries. in the present study, we evaluated antagonism and competitive interactions between 98 fungal endophytes and altern ...	2015	26273250
microbispora sp. lgmb259 endophytic actinomycete isolated from vochysia divergens (pantanal, brazil) producing β-carbolines and indoles with biological activity.	endophytic actinomycetes encompass bacterial groups that are well known for the production of a diverse range of secondary metabolites. vochysia divergens is a medicinal plant, common in the "pantanal" region (brazil) and was focus of many investigations, but never regarding its community of endophytic symbionts. during a screening program, an endophytic strain isolated from the v. divergens, was investigated for its potential to show biological activity. the strain was characterized as microbis ...	2015	25385358
identification of genes associated with asexual reproduction in phyllosticta citricarpa mutants obtained through agrobacterium tumefaciens transformation.	phyllosticta citricarpa is the epidemiological agent of citrus black spot (cbs) disease, which is responsible for large economic losses worldwide. cbs is characterized by the presence of spores (pycnidiospores) in dark lesions of fruit, which are also responsible for short distance dispersal of the disease. the identification of genes involved in asexual reproduction of p. citricarpa can be an alternative for directional disease control. we analyzed a library of mutants obtained through agrobact ...	2016	27664732
mating type and simple sequence repeat markers indicate a clonal population of phyllosticta citricarpa in florida.	phyllosticta citricarpa, the citrus black spot pathogen, was first identified in florida in march 2010. subsequently, this pathogen has become established in florida but can be easily confused with the endemic nonpathogenic citrus endophyte p. capitalensis. in this study, the mating-type (mat) loci of p. citricarpa and p. capitalensis were identified via draft genome sequencing and were characterized at the structural and sequence levels. p. citricarpa was determined to have an idiomorphic, hete ...	2016	27348343
proteomic response of the phytopathogen phyllosticta citricarpa to antimicrobial volatile organic compounds from saccharomyces cerevisiae.	volatile organic compounds (vocs) released by saccharomyces cerevisiae inhibit plant pathogens, including the filamentous fungus phyllosticta citricarpa, causal agent of citrus black spot. vocs mediate relevant interactions between organisms in nature, and antimicrobial vocs are promising, environmentally safer fumigants to control phytopathogens. as the mechanisms by which vocs inhibit microorganisms are not well characterized, we evaluated the proteomic response in p. citricarpa after exposure ...	2016	26805613
sexual reproduction in the citrus black spot pathogen, phyllosticta citricarpa.	citrus black spot (phyllosticta citricarpa) causes fruit blemishes and premature fruit drop, resulting in significant economic losses in citrus growing areas with summer rainfall across the globe. the mating type locus of p. citricarpa has recently been characterized, revealing the heterothallic nature of this pathogen. however, insight into the occurrence of mating and the impact of completing the sexual cycle of p. citricarpa was lacking. to investigate the occurrence and impact of sexual repr ...	2017	28387613
biological activity of diaporthe terebinthifolii extracts against phyllosticta citricarpa.		2017	28158748
a global perspective on the population structure and reproductive system of phyllosticta citricarpa.	the citrus pathogen phyllosticta citricarpa was first described 117 years ago in australia, and subsequently from the summer rainfall citrus-growing regions in china, africa, south america and recently the united states of america (usa). limited information is available on the pathogen's population structure, mode of reproduction and introduction pathways, which were investigated by genotyping 383 isolates representing 12 populations from south africa, usa, australia, china and brazil. populatio ...	2017	28134595
a statistical evaluation of methods of in-vitro growth assessment for phyllosticta citricarpa: average colony diameter vs. area.	fungal growth inhibition on solid media has been historically measured and calculated based on the average of perpendicular diameter measurements of growth on fungicide amended media. we investigated the sensitivity of the calculated area (da) and the measured area (ma) for assessing fungicide growth inhibition of the ascomycete, phyllosticta citricarpa on solid media. both the calculated, da and the actual measured area, ma were adequate for distinguishing significant treatment effects of fungi ...	2017	28125679
diaporthe endophytica and d. terebinthifolii from medicinal plants for biological control of phyllosticta citricarpa.	the citrus industry is severely affected by citrus black spot (cbs), a disease caused by the pathogen phyllosticta citricarpa. this disease causes loss of production, decrease in the market price of the fruit, and reduction in its export to the european union. currently, cbs disease is being treated in orchards with various pesticides and fungicides every year. one alternative to cbs disease control without harming the environment is the use of microorganisms for biological control. diaporthe en ...	2017	27242153