Publications

the fine structure of micrococcus radiophilus and micrococcus radioproteolyticus.	the radiation resistant bacteria micrococcus radiophilus and m. radioproteolyticus were studied by thin sectioning and freez-etching techniques and the two species were found to be similar in the fine structure. the only significant difference was in the appearance of the surfaces of the cell walls in freeze-etched preparations. since the two species, together with m. radiodurans, possess a unique cell wall structure and a cell wall peptidoglycan, which is different from that of other micrococci ...	1976	1275640
influence of repeated lyophilization on the survival of deinococcus proteolyticus, micrococcus luteus and escherichia coli.	repeated lyophilization of deinococcus proteolyticus, micrococcus luteus and escherichia coli cells results in a successive decrease of their survival. the survival curve is exponential with e. coli and m. luteus, and sigmoidal with a broad shoulder with d. proteolyticus both after repeated lyophilization and after uv- or gamma-irradiation. when cells were subjected to gamma-irradiation after a 20-fold freeze-drying, the corresponding survival curve became exponential without the shoulder. hence ...	1991	1841852
sequencing of heat shock protein 70 (dnak) homologs from deinococcus proteolyticus and thermomicrobium roseum and their integration in a protein-based phylogeny of prokaryotes.	the 70-kda heat shock protein (hsp70) sequences define one of the most conserved proteins known to date. the hsp70 genes from deinococcus proteolyticus and thermomicrobium roseum, which were chosen as representatives of two of the most deeply branching divisions in the 16s rrna trees, were cloned and sequenced. hsp70 from both these species as well as thermus aquaticus contained a large insert in the n-terminal quadrant, which has been observed before as a unique characteristic of gram-negative ...	1997	8990285
phylogenetic diversity of the deinococci as determined by 16s ribosomal dna sequence comparison.	16s ribosomal dna (rdna) sequences were determined for the five species of the genus deinococcus (deinococcus erythromyxa, deinococcus proteolyticus, deinococcus radiodurans, deinococcus radiophilus, and deinococcus radiopugnans) and the single species of the genus. deinobacter (deinobacter grandis). with the exception of deinococcus erythromyxa, the deinococci form a coherent phylogenetic cluster which is related to the thermus-meiothermus lineage. an analysis of the 16s rdna sequence of deinoc ...	1997	9103641
the sequences of heat shock protein 40 (dnaj) homologs provide evidence for a close evolutionary relationship between the deinococcus-thermus group and cyanobacteria.	the genes encoding for heat shock protein 40 (hsp40 or dnaj) homologs were cloned and sequenced from the archaebacterium halobacterium cutirubrum and the eubacterium deinococcus proteolyticus to add to sequences from the gene banks. these genes were identified downstream of the hsp70 (or dnak) genes in genomic fragments spanning this region and, as in other prokaryotic species, hsp70-hsp40 genes are likely part of the same operon. the hsp40 homolog from d. proteolyticus was found to be lacking a ...	1997	9236279
distinctive protein signatures provide molecular markers and evidence for the monophyletic nature of the deinococcus-thermus phylum.	the deinococcus-thermus group of species is currently recognized as a distinct phylum solely on the basis of their branching in 16s rrna trees. no unique biochemical or molecular characteristics that can distinguish this group from all other bacteria are known at present. in this work, we describe eight conserved indels (viz., inserts or deletions) in seven widely distributed proteins that are distinctive characteristics of the deinococcus-thermus phylum but are not found in any other group of b ...	2004	15126471
a ring-like nucleoid is not necessary for radioresistance in the deinococcaceae.	transmission electron microscopy images of deinococcus radiodurans r1 suggest that the nucleoid of this species exists as a "ring-like" body, and have led to speculation that this structure contributes to the radioresistance of the species. since extreme radioresistance is characteristic of six other species of deinococcus, we have attempted to correlate nucleoid morphology and radioresistance by determining whether the genomic dna of each of these species exhibit similar structures.	2005	15799787
deinococcus ficus sp. nov., isolated from the rhizosphere of ficus religiosa l.	a pale-pink strain (cc-fr2-10t) from the rhizosphere of the sacred tree ficus religiosa l. in taiwan was investigated by using a polyphasic taxonomic approach. the cells were gram-positive, rod-shaped and non-spore-forming. phylogenetic analyses using the 16s rrna gene sequence of the isolate indicated that the organism belongs to the genus deinococcus, the highest sequence similarities being found with deinococcus grandis (96.1 %), deinococcus radiodurans (94.3 %), deinococcus radiopugnans (93. ...	2006	16585695
identification and properties of the deinococcus grandis and deinococcus proteolyticus single-stranded dna binding proteins (ssb).	to study the biochemical properties of ssb's from deinococcus grandis (dgrssb) and deinococcus proteolyticus (dprssb), we have cloned the ssb genes obtained by pcr and have developed escherichia coli overexpression systems. the genes consist of an open reading frame of 891 (dgrssb) and 876 (dprssb) nucleotides encoding proteins of 296 and 291 amino acids with a calculated molecular mass of 32.29 and 31.33 kda, respectively. the amino-acid sequence of dgrssb exhibits 45%, 44% and 82% identity and ...	2007	17325748
deinococcus piscis sp. nov., a radiation-resistant bacterium isolated from a marine fish.	a radiation-resistant, gram-stain-positive, non-motile, non-sporulating, aerobic, coccoid bacterium, strain 3ax(t), was isolated from a marine fish (black pomfret, parastromateus niger), with radiation as a selective pressure. phylogenetic analysis based on 16s rrna gene sequences showed that strain 3ax(t) exhibited highest similarity (97.9 %) with deinococcus proteolyticus dsm 20540(t). the deltat(m) for dna-dna hybridization of d. proteolyticus dsm 20540(t) and strain 3ax(t) was 15.3 degrees c ...	2009	19625434
evolution of lysine biosynthesis in the phylum deinococcus-thermus.	thermus thermophilus biosynthesizes lysine through the α-aminoadipate (aaa) pathway: this observation was the first discovery of lysine biosynthesis through the aaa pathway in archaea and bacteria. genes homologous to the t. thermophilus lysine biosynthetic genes are widely distributed in bacteria of the deinococcus-thermus phylum. our phylogenetic analyses strongly suggest that a common ancestor of the deinococcus-thermus phylum had the ancestral genes for bacterial lysine biosynthesis through ...	2012	22645699
complete genome sequence of the orange-red pigmented, radioresistant deinococcus proteolyticus type strain (mrp(t)).	deinococcus proteolyticus (ex kobatake et al. 1973) brook and murray 1981 is one of currently 47 species in the genus deinococcus within the family deinococcaceae. strain mrp(t) was isolated from feces of lama glama and possesses extreme radiation resistance, a trait is shares with various other species of the genus deinococcus, with d. proteolyticus being resistant up to 1.5 mrad of gamma radiation. strain mrp(t) is of further interest for its carotenoid pigment. the genome presented here is on ...	2012	22768367
doping polypyrrole films with 4-n-pentylphenylboronic acid to enhance affinity towards bacteria and dopamine.	here we demonstrate the use of a functional dopant as a fast and simple way to tune the chemical affinity and selectivity of polypyrrole films. more specifically, a boronic-functionalised dopant, 4-n-pentylphenylboronic acid (pba), was used to provide to polypyrrole films with enhanced affinity towards diols. in order to prove the proposed concept, two model systems were explored: (i) the capture and the electrochemical detection of dopamine and (ii) the adhesion of bacteria onto surfaces. the c ...	2016	27875555