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phylogenetic diversity of klebsiella pneumoniae and klebsiella oxytoca clinical isolates revealed by randomly amplified polymorphic dna, gyra and parc genes sequencing and automated ribotyping.the infra-specific phylogenetic diversity and genetic structure of both klebsiella pneumoniae and klebsiella oxytoca was investigated using a combination of randomly amplified polymorphic dna (rapd) analysis, sequencing of gyra and parc genes, and automated ribotyping. after rapd analysis with four independent primers of 120 clinical isolates collected from 22 european hospitals in 13 countries, k. pneumoniae isolates fell into three clusters and k. oxytoca isolates fell into two clusters, while ...200111411715
phylogenetic analyses of klebsiella species delineate klebsiella and raoultella gen. nov., with description of raoultella ornithinolytica comb. nov., raoultella terrigena comb. nov. and raoultella planticola comb. nov.the phylogenetic relationships of the type strains of 9 klebsiella species and 20 species from 11 genera of the family enterobacteriaceae were investigated by performing a comparative analysis of the sequences of the 16s rrna and rpob genes. the sequence data were phylogenetically analysed by the neighbourjoining and parsimony methods. the phylogenetic inference of the sequence comparison confirmed that the genus klebsiella is heterogeneous and composed of species which form three clusters that ...200111411716
klebsiella pneumoniae produces no histamine: raoultella planticola and raoultella ornithinolytica strains are histamine producers.histamine fish poisoning is caused by histamine-producing bacteria (hpb). klebsiella pneumoniae and klebsiella oxytoca are the best-known hpb in fish. however, 22 strains of hpb from fish first identified as k. pneumoniae or k. oxytoca by commercialized systems were later correctly identified as raoultella planticola (formerly klebsiella planticola) by additional tests. similarly, five strains of raoultella ornithinolytica (formerly klebsiella ornithinolytica) were isolated from fish as new hpb. ...200212089029
[effect of culture media on surface and enzymatic activity in klebsiella species].the effect of three complex media (km) [mueller-hinton broth (mhb), brain heart infusion (msi) and proteose peptone (pp)] and one mineral medium (mm) on surface and enzyme activities of five strains klebsiella species was studied. klebsiella oxytoca and klebsiella ornithinolytica had a marked hydrophobic character after growth in mhb, msi and mm, klebsiella terrigena only in pp. k. oxytoca and k. ornithinolytica had a higher motility after cultivation in km compared with mm, the motility of k. t ...200212184188
enterobacterial repetitive intergenic consensus 1r pcr assay for detection of raoultella sp. isolates among strains identified as klebsiella oxytoca in the clinical laboratory.the enterobacterial repetitive intergenic consensus 1r pcr method, which provided recognizable profiles for reference strains of the three species of raoultella and the two genetic groups of klebsiella oxytoca, was applied to 19 clinical isolates identified as k. oxytoca. by this method, as confirmed by species-specific gene sequencing, two raoultella ornithinolytica and two unclassifiable k. oxytoca isolates were identified.200312682174
genetic and biochemical characterization of the chromosomal class a beta-lactamases of raoultella (formerly klebsiella) planticola and raoultella ornithinolytica.enterobacterial strains of raoultella spp. display a penicillinase-related beta-lactam resistance pattern suggesting the presence of a chromosomal bla gene. from whole-cell dna of raoultella planticola strain atcc 33531(t) and raoultella ornithinolytica strain atcc 31898(t), bla genes were cloned and expressed into escherichia coli. each gene encoded an ambler class a beta-lactamase, named pla-1 and orn-1 for r. planticola and r. ornithinolytica, respectively. these beta-lactamases (291 amino ac ...200414693555
recommended test panel for differentiation of klebsiella species on the basis of a trilateral interlaboratory evaluation of 18 biochemical tests.klebsiella pneumoniae and klebsiella oxytoca are the two most frequently encountered klebsiella species giving rise to infections in humans, but other klebsiella species can also be found in clinical specimens: klebsiella ozaenae, klebsiella rhinoscleromatis, klebsiella terrigena, klebsiella planticola, klebsiella ornithinolytica, and enterobacter aerogenes (klebsiella mobilis). however, many of these species are indistinguishable by the conventional methods employed routinely in the clinical mi ...200415297514
alkali-resistant bacteria in root canal systems.the aim of this study was to isolate and identify alkali-resistant bacteria from the dentin of infected root canals. bacteria from homogenized dentin powder made up from infected root canal walls from human teeth were cultured on buffer-enriched brain heart infusion agar supplemented with 4% sheep blood (bhi-blood agar), adjusted to ph 7.0, 9.0 or 10.0. incubation took place for 7 days at 37 degrees c in an anaerobic glove box. bacterial strains selected according to colony and morphology were s ...200415491465
histidine decarboxylase in enterobacteriaceae revisited.with a modification of taylor's decarboxylation broth, histidine decarboxylase was detected in enterobacter aerogenes, morganella morganii, raoultella ornithinolytica, and some strains of citrobacter youngae and raoultella planticola. this method provides a useful confirmatory test for identification of e. aerogenes strains.200415583342
the binding of mbl to common bacteria in infectious diseases of children.to purify mannan-binding lectin (mbl) from human serum and detect its binding ability to several kinds of bacteria common in infectious diseases of children.200515593393
gram-negative bacteria from the camel tick hyalomma dromedarii (ixodidae) and the chicken tick argas persicus (argasidae) and their antibiotic sensitivities.a total of nine species of gram-negative bacteria were isolated from organs and haemolymph of the hard tick hyalomma (hyalomma) dromedarii and the soft tick argas (persicargas) persicus. four species namely serratia liquefaciens, stenotrophomonas maltophilia, klebsiella ornithinolytica and aeromonas hydrophila were isolated from h. dromedarii and five species namely rahnella aquatilis, pseudomonas fluorescens, enterobacter cloacae, chryseomonas luteola and chryseobacterium meningosepticum were i ...200515880998
occurrence and diversity of integrons and beta-lactamase genes among ampicillin-resistant isolates from estuarine waters.the aim of the present study was to assess the occurrence and molecular diversity of beta-lactamase genes and integrons among gram-negative ampicillin-resistant bacteria from ria de aveiro. ampicillin-resistant isolates were selected and subjected to genotyping using rep-pcr. representatives from each rep pattern were affiliated with the following taxa by sequencing the 16s rrna gene: aeromonas caviae, a. hydrophila, a. media, a. molluscorum, a. veronii, a. salmonicida, aeromonas sp., pseudomona ...200617125975
benzoic acid-degrading bacteria from the intestinal tract of macrotermes michaelseni sjöstedt.the intestinal tracts of termites host a wide variety of microbial symbionts, which have been implicated in degradative processes. in this study, a fungus-cultivating termite, macrotermes michaelseni was found to harbor 2.2 x 10(6) bacterial cells per ml of gut homogenates capable of degrading benzoic acid. two benzoic acid degrading bacteria were isolated from the highest dilution of gut homogenates in oxic media with benzoic acid as the sole carbon source. isolate cbc was related to stenotroph ...200717304624
monitoring and identification of bacteria associated with safety concerns in the manufacture of são jorge, a portuguese traditional cheese from raw cow's milk.the aim of this study was to assess the hygienic quality of raw milk used in the manufacture of são jorge, a protected denomination of origin portuguese semihard cheese, as well as to ascertain the sanitary conditions prevailing during its processing. viable counts of enterobacteriaceae and micrococcaceae were accordingly obtained, pertaining to 21 independent batches (including samples of raw milk, curd, and cheeses after 1, 3, and 4 months of ripening), from 7 dairy farms. standard plate count ...200818522034
how to identify raoultella spp. including r. ornithinolytica isolates negative for ornithine decarboxylase? the reliability of the chromosomal bla gene.although raoultella planticola and raoultella ornithinolytica were described more than 20 years ago, identifying them remains difficult. the reliability of the chromosomal bla gene for this identification was evaluated in comparison with that of the 16s rdna and rpob genes in 35 raoultella strains from different origins. of the 26 strains previously identified as r. planticola by biochemical tests alone or in association with molecular methods, 21 harboured a bla gene with 99.8% identity with th ...200818694786
analysis of the 16s-23s rrna gene internal transcribed spacer region in klebsiella species.the 16s-23s rrna gene internal transcribed spacer (its) regions of klebsiella spp., including klebsiella pneumoniae subsp. pneumoniae, klebsiella pneumoniae subsp. ozaenae, klebsiella pneumoniae subsp. rhinoscleromatis, klebsiella oxytoca, klebsiella planticola, klebsiella terrigena, and klebsiella ornithinolytica, were characterized, and the feasibility of using its sequences to discriminate klebsiella species and subspecies was explored. a total of 336 its sequences from 21 representative stra ...200818753345
acute suppuration of the pancreatic duct in a patient with tropical pancreatitis.pancreatic sepsis secondary to infected necrosis, pseudocyst, or pancreatic abscess is a well-known clinical entity. acute suppuration of the pancreatic duct (aspd) in the setting of chronic calcific pancreatitis and pancreatic ductal obstruction with septicemia is a rare complication that is seldom reported. it is our aim to report a case of aspd with klebsiella ornithinolytica, in the absence of pancreatic abscess or infected necrosis.200821490834
enteric fever-like syndrome caused by raoultella ornithinolytica (klebsiella ornithinolytica). 200919144809
diversity of hydrocarbon-degrading klebsiella strains isolated from hydrocarbon-contaminated estuaries.to investigate the diversity and the catabolic capacity of oil-degrading klebsiella strains isolated from hydrocarbon-contaminated sediments in santos-são vicente estuary systems in brazil.200919187158
atrazine degradation by stable mixed cultures enriched from agricultural soil and their characterization.the aim of this work was to enrich stable mixed cultures from atrazine-contaminated soil. the cultures were examined for their atrazine biodegradation efficiencies in comparison with j14a, a known atrazine-degrading strain of agrobacterium radiobacter. the cultures were also characterized to identify community structure and bacterial species present.200919191954
[giant renal cyst as cause of colic obstruction].a 97-year old woman presented with a 2-months history of asthenia, loss of appetite, nauseas and intermittent diarrhea. she presented an important colic distension associated to an inflammatory syndrome. microbiological documentation (blood, urine, saddles stool) was negative. abdominal computed tomography (ct) showed a 15 cm of diameter (giant) right cortical renal cyst with colic obstruction by external colic compression. a percutaneous cyst drainage was performed allowing the evacuation of 1, ...200919517907
first descriptions of blakpc in raoultella spp. (r. planticola and r. ornithinolytica): report from the sentry antimicrobial surveillance program.two strains of raoultella planticola and one of raoultella ornithinolytica showing carbapenem resistance were recovered from patients hospitalized in new jersey and ohio. all patients had received previous antimicrobial treatment, including carbapenems. these strains harbored bla(kpc-2) and bla(kpc-3). carbapenemase genes were embedded in isoforms of tn4401 and were plasmidic and chromosomal in location.200919812278
chromium (vi) biotransformation by beta- and gamma-proteobacteria from natural polluted environments: a combined biological and chemical treatment for industrial wastes.the high solubility of cr(vi) in aqueous systems, together with carcinogenic and mutagenic effects on living organisms, make industrial effluents receive specific treatments for cr(vi) elimination. biotreatments, based on biotransformation of cr(vi) to cr(iii) which is immobilized as cr(oh)(3), are the most effective methods for the removal of cr(vi) concentrations below 2mm. the aim of our study is the application of pure or mixed bacterial cultures for cr(vi) biotransformation followed by chem ...201019879691
raoultella ornithinolytica bacteremia in an infant with visceral heterotaxy.a case of raoultella ornithinolytica bacteremia in an infant with visceral heterotaxy is reported. physical examination was remarkable for markedly red skin flushing, not unlike that seen during histamine fish poisoning. r. ornithinolytica is a histamine-producing bacterium recently elucidated as a major cause of histamine fish poisoning. only 2 other cases of human infection by r. ornithinolytica have been reported.201020072080
gut bacterium of dendrobaena veneta (annelida: oligochaeta) possesses antimycobacterial activity.the new bacterial strain with antimycobacterial activity has been isolated from the midgut of dendrobaena veneta (annelida). biochemical and molecular characterization of isolates from 18 individuals identified all as raoultella ornithinolytica genus with 99% similarity. the bacterium is a possible symbiont of the earthworm d. veneta. the isolated microorganism has shown the activity against four strains of fast-growing mycobacteria: mycobacterium butiricum, mycobacterium jucho, mycobacterium sm ...201020452357
evaluation of three phenotypic identification systems for clinical isolates of raoultella ornithinolytica.raoultella spp. have recently been separated from the genus klebsiella based on their molecular characteristics. it was discovered that raoultella ornithinolytica can be misidentified as klebsiella oxytoca by commonly used phenotypic identification systems. therefore, this study evaluated the ability of three phenotypic systems to identify r. ornithinolytica compared with the genotypic methods sequence-specific primer pcr (ssp-pcr), 16s rrna gene sequence analysis using the microseq 500 system16 ...201121212145
a rare human infection of raoultella ornithinolytica in a diabetic foot lesion. 201121245606
spatiotemporal development of the bacterial community in a tubular longitudinal microbial fuel cell.the spatiotemporal development of a bacterial community in an exoelectrogenic biofilm was investigated in sucrose-fed longitudinal tubular microbial fuel cell reactors, consisting of two serially connected modules. the proportional changes in the microbial community composition were assessed by polymerase chain reaction-denaturing gradient gel electrophoresis (dgge) and dna sequencing in order to relate them to the performance and stability of the bioelectrochemical system. the reproducibility o ...201121400098
the activity of antimicrobial peptide s-thanatin is independent on multidrug-resistant spectrum of bacteria.in this study, the activity of s-thanatin (an analog of antimicrobial peptide derived from thanatin) against different bacterial pathogens frequently which can cause therapeutic problems was tested. the result showed minimal inhibitory concentrations (mics) of s-thanatin against all isolates of the escherichia coli, klebsiella pneumoniae, enterobacter cloacae, enterobacter aerogenes, klebsiella ornithinolytica and klebsiella oxytoca were in the range of 4-16µg/ml, no matter which antibiotic the ...201121453736
analysis of the bacterial diversity existing on animal hide and wool: development of a preliminary pcr-restriction fragment length polymorphism fingerprint database for identifying isolates.twenty-one bacterial strains were isolated from imported cattle hide and rabbit wool using two types of media, nutrient broth, and nutrient broth with serum. the bacteria identified were brevibacillus laterosporus, leclercia adecarboxylata, peptococcus niger, bacillus circulans, raoultella ornithinolytica, bacillus subtilis, bacillus cereus, bacillus thermobacillus, bacillus choshinensis, bacillus sphaericus, acinetobacter haemolyticus, sphingomonas paucimobilis, bacillus thuringiensis, staphylo ...201223451394
characterization of bacterial biofilms formed on urinary catheters.the formation of bacterial biofilms on urinary catheters is a leading cause of urinary tract infections in intensive care units. cytobacteriological examination of urine from patients is often misleading, due to the formation of these biofilms. therefore, characterizing these biofilms and identifying the bacterial species residing on the surface of catheters are of major importance.201222325732
anti-candida albicans action of the glyco-protein complex purified from metabolites of gut bacterium raoultella ornithinolytica isolated from earthworms dendrobaena veneta.the aim of our research was to isolate the compounds from the metabolites of raoultella ornithinolytica with the activity against candida albicans and to analyse the action of the compounds on the metabolic activity and morphology of the fungus cells.201222816366
in vitro and in vivo antimicrobial activity of propolis on the microbiota from gastrointestinal tract of chickens.the aim of this study was to examine the effect of propolis extracts on the microbial colonization of chicken gastrointestinal tract in vivo. the propolis was administered to both feed mixtures in various amounts except of the control group. the addition of 150 mg propolis to 1 kg of feed was included in the first experimental group, the addition of 450 mg.kg(-1) in the second experimental group, the addition of 600 mg.kg(-1) the third experimental group and 800 mg kg(-1) in the fourth one. the ...201222702827
biogenic amine content, histamine-forming bacteria, and adulteration of pork in tuna sausage products.twenty-five tuna sausage products were purchased from retail markets in taiwan. the rates of occurrence of biogenic amines, histamine-forming bacteria, and adulteration by pork and poultry were determined. the average content of various biogenic amines in all tested samples was less than 2.0 mg/100 g (<0.05 to 1.85 mg/100 g). thirteen histamine-producing bacterial strains isolated from tested samples produced 12.1 to 1,261 ppm of histamine in trypticase soy broth supplemented with 1.0% l-histidi ...201223043830
yersinia enterocolitica in sheep--a high frequency of biotype 1a.pigs are regarded as the main reservoir for human pathogenic yersinia enterocolitica, which is dominated by bioserotype 4/o:3. other animals, including sheep, have occasionally been reported as carriers of pathogenic strains of y. enterocolitica. to our knowledge, this is the first study performed in the nordic countries in which the presence of y. enterocolitica in sheep is investigated.201222748116
raoultella ornithinolytica bacteremia in cancer patients: report of three cases.raoultella ornithinolytica is a gram-negative aerobic bacillus reclassified in the new genus from the klebsiella species based on new genetic approaches; however, human infections caused by r. ornithinolytica are rare. we herein report three cases of r. ornithinolytica bacteremia associated with biliary tract infections in cancer patients. r. ornithinolytica can be a causative pathogen of biliary tract infection in cancer patients.201223154732
isolation and characterization of a nitrobenzene-degrading bacterium klebsiella ornithinolytica nb1 from aerobic granular sludge.strain nb1 was isolated from aerobic granular sludge capable of degrading nitrobenzene. effects of several factors including ph, temperature, salinity, and second carbon/nitrogen source as co-substrate on the biodegradation of nitrobenzene by strain nb1 were investigated. results showed that the optimal conditions for the biodegradation of nitrobenzene by strain nb1 were at ph 7.0 and 28-35°c. under these conditions, the biodegradation rate of nitrobenzene could reach 9.29mgl(-1)h(-1) when the i ...201222342585
determination and frying loss of histamine in striped marlin fillets implicated in a foodborne poisoning.an incident of foodborne poisoning causing illness in 67 victims due to ingestion of fried fish fillets occurred in june 2011, in southern taiwan. of the five suspected fish fillets, one fried sample contained 62.0 mg/100 g and one raw sample contained 89.6 mg/100 g histamine, levels which are greater than the potential hazard action level (50 mg/100 g) in most illness cases. given the allergy-like symptoms of the victims and the high histamine content in the suspected fish samples, this foodbor ...201323643129
complete genome sequence of raoultella ornithinolytica strain b6, a 2,3-butanediol-producing bacterium isolated from oil-contaminated soil.here we report the full genome sequence of raoultella ornithinolytica strain b6, a gram-negative aerobic bacillus belonging to the family enterobacteriaceae. this 2,3-butanediol-producing bacterium was isolated from oil-contaminated soil on backwoon mountain in south korea. strain b6 contains 5,398,151 bp with 4,909 protein-coding genes, 104 structural rnas, and 55.88% g+c content.201323814034
[raoultella ornithinolytica in urinary tract infections. clinical and microbiological study of a series of 4 oncologic patients]. 201323332629
first report of blandm-1 in raoultella ornithinolytica. 201323165470
differentiation of raoultella ornithinolytica/planticola and klebsiella oxytoca clinical isolates by matrix-assisted laser desorption/ionization-time of flight mass spectrometry.ninety-nine clinical isolates previously identified as klebsiella oxytoca were evaluated using matrix-assisted laser desorption/ionization-time of flight mass spectrometry (maldi-tof ms). eight isolates were identified as raoultella spp., being 5 raoultella spp. and 3 k. oxytoca, by 16s rrna sequencing. these isolates were correctly identified by applying the 10% differential rule for the maldi-tof ms score values. this approach might be useful to discriminate raoultella species from k. oxytoca.201323375086
identification of enterobacteriaceae by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry using the vitek ms system.this multicenter study evaluated the accuracy of matrix-assisted laser desorption/ionization time-of-flight (maldi-tof) mass spectrometry identifications from the vitek ms system (biomérieux, marcy l'etoile, france) for enterobacteriaceae typically encountered in the clinical laboratory. enterobacteriaceae isolates (n = 965) representing 17 genera and 40 species were analyzed on the vitek ms system (database v2.0), in accordance with the manufacturer's instructions. colony growth (≤72 h) was app ...201323818163
antifungal and anticancer effects of a polysaccharide-protein complex from the gut bacterium raoultella ornithinolytica isolated from the earthworm dendrobaena veneta.the polysaccharide-protein complex (ppc) isolated from metabolites of gut bacteria raoultella ornithinolytica from dendrobaena veneta earthworms exhibits activity against candida albicans, in breast ductal carcinoma (line t47d) and in the endometrioid ovarian cancer line (tov-112d) in vitro. the action against c. albicans was analyzed using light, sem, tem, and afm microscopes. the changes observed indicated two directions of the action of the complex, that is, disturbance of metabolic activity ...201323794449
identification of the enterobacteriaceae in montasio cheese and assessment of their amino acid decarboxylase activity.the aim of the study was to identify the species of enterobacteriaceae present in montasio cheese and to assess their potential to produce biogenic amines. plate count methods and an enterobacterial repetitive intergenic consensus polymerase chain reaction (eric-pcr) approach, combined with 16s rdna sequencing, were used to investigate the enterobacteriaceae community present during the cheesemaking and ripening of 6 batches of montasio cheese. additionally, the potential decarboxylation abiliti ...201323298547
detection of sulphate-reducing bacteria in human saliva.the aim of the current study was to investigate the presence of sulphate-reducing bacteria (srb) in human saliva and correlate with oral and systemic conditions.201323638810
microbial diversity and dynamics during the production of may bryndza cheese.diversity and dynamics of microbial cultures were studied during the production of may bryndza cheese, a traditional slovak cheese produced from unpasteurized ewes' milk. quantitative culture-based data were obtained for lactobacilli, lactococci, total mesophilic aerobic counts, coliforms, e. coli, staphylococci, coagulase-positive staphylococci, yeasts, fungi and geotrichum spp. in ewes' milk, curd produced from it and ripened for 0 - 10 days, and in bryndza cheese produced from the curd, in th ...201424291178
draft genome sequence of raoultella ornithinolytica tnt, a trinitrotoluene-denitrating and plant growth-promoting strain isolated from explosive-contaminated soil.we report the draft genome of raoultella ornithinolytica tnt, a gram-negative bacterium of the enterobacteriaceae isolated from military soil in belgium. strain tnt uses nitrite released from trinitrotoluene (tnt) for growth and is a potent plant growth promoter. an analysis of its 5.6-mb draft genome will bring insights into tnt degradation-reinforcing bioremediation applications.201424874678
ndm-1-producing strains, family enterobacteriaceae, in hospital, beijing, china. 201424456600
cloning and sequence analysis demonstrate the chromate reduction ability of a novel chromate reductase gene from serratia sp.the chrt gene encodes a chromate reductase enzyme which catalyzes the reduction of cr(vi). the chromate reductase is also known as flavin mononucleotide (fmn) reductase (fmn_red). the aim of the present study was to clone the full-length chrt dna from serratia sp. cqmus2 and analyze the deduced amino acid sequence and three-dimensional structure. the putative chrt gene fragment of serratia sp. cqmus2 was isolated by polymerase chain reaction (pcr), according to the known fmn_red gene sequence fr ...201425667630
use of cellulolytic marine bacteria for enzymatic pretreatment in microalgal biogas production.in this study, we designed and evaluated a microalgal pretreatment method using cellulolytic bacteria that naturally degrades microalgae in their native habitat. bacterial strains were isolated from each of two mollusk species in a medium containing 1% carboxymethyl cellulose agar. we selected nine bacterial strains that had endoglucanase activity: five strains from mytilus chilensis, a chilean mussel, and four strains from mesodesma donacium, a clam found in the southern pacific. these strains ...201424795376
molecular characterization of klebsiella pneumoniae carbapenemase (kpc)-producing enterobacteriaceae in ontario, canada, 2008-2011.due to the lack of detailed reports of klebsiella pneumoniae carbapenemase (kpc)-producing enterobacteria in ontario, canada, we perform a molecular characterization of kpc-producing enterobacteriaceae submitted to the provincial reference laboratory from 2008 to 2011. susceptibility profiles were accessed by e-test. molecular types of isolates were determined by pulse-field gel electrophoresis (pfge) and multilocus sequence typing. screening of ß-lactamase genes was performed by multiplex pcr a ...201425549365
clinical characteristics of raoultella ornithinolytica bacteremia: a case series and literature review.raoultella ornithinolytica is a rare pathogen in human infection and bacteremic cases had been scarcely reported. for further comprehension of the rare infection, we summarized clinical characteristics of 6 cases that were detected at our medical facility and 5 cases from previous literature. the most common infectious focus was biliary infection and elderly patients with a history of any biliary intervention or malignancy were considered to be at a great risk for the infection. the prognosis of ...201425012469
antitumour and apoptotic effects of a novel tris-peptide complex obtained after isolation of raoultella ornithinolytica extracellular metabolites.the characterization of the antitumour activity and chemical identification of the compounds obtained after the isolation of extracellular metabolites of bacteria raoultella ornithinolytica.201525809524
complete genome sequence of raoultella ornithinolytica strain s12, a lignin-degrading bacterium isolated from forest soil.we report the complete genome sequence of raoultella ornithinolytica strain s12, isolated from a soil sample collected from areas bordering rotten wood and wet soil on mt. zijin, nanjing. the complete genome of this bacterium may contribute toward the discovery of efficient lignin-degrading pathways.201525792045
raoultella ornithinolytica causing fatal sepsis. 201525626959
clinical characteristics of raoultella ornithinolytica bacteremia.raoultella ornithinolytica is not well known as a clinical pathogen. we performed a retrospective review of r. ornithinolytica bacteremia to investigate its clinical features, antimicrobial susceptibility, and overall patient outcomes.201525367410
production of plasmid-encoding ndm-1 in clinical raoultella ornithinolytica and leclercia adecarboxylata from china.raoultella ornithinolytica ynkp001 and leclercia adecarboxylata p10164, which harbor conjugative plasmids pynkp001-ndm and pp10164-ndm, respectively, were isolated from two different chinese patients, and their complete nucleotide sequences were determined. production of ndm-1 enzyme by these plasmids accounts for the carbapenem resistance of these two strains. this is the first report of bla ndm in l. adecarboxylata and third report of this gene in r. ornithinolytica. pynkp001-ndm is very simil ...201526052314
genomics and ecophysiology of heterotrophic nitrogen-fixing bacteria isolated from estuarine surface water.the ability to reduce atmospheric nitrogen (n2) to ammonia, known as n2 fixation, is a widely distributed trait among prokaryotes that accounts for an essential input of new n to a multitude of environments. nitrogenase reductase gene (nifh) composition suggests that putative n2-fixing heterotrophic organisms are widespread in marine bacterioplankton, but their autecology and ecological significance are unknown. here, we report genomic and ecophysiology data in relation to n2 fixation by three e ...201526152586
emergence of raoultella ornithinolytica coproducing imp-4 and kpc-2 carbapenemases in china.we report here the emergence of seven imp-4-producing raoultella ornithinolytica isolates obtained from one patient. all isolates carried the blaimp-4 carbapenemase gene, five isolates also carried blashv-12, four contained blatem-1, and one contained blaoxa-1. notably, the r. ornithinolytica isolate ro25724 also expressed klebsiella pneumoniae carbapenemase (kpc)-2. the blakpc-2 gene was located on a tn3-tn4401 integration structure on a plasmid of ∼450 kb. this is the first description of the ...201526282422
antibiotic resistance of gram-negative benthic bacteria isolated from the sediments of kardzhali dam (bulgaria).the aim of the present study was to carry out a preliminary assessment for the occurrence of bacterial strains resistant to frequently used antibiotics in the sediments beneath the sturgeon cage farm in kardzhali dam (bulgaria). samples were taken from the top 2 cm of sediments under a fish farm and from a control station in the aquatory of the reservoir in the period july-october 2011. surveillance of bacterial susceptibility to 16 antimicrobial agents was performed for 160 gram-negative strain ...201526019641
emergence of raoultella ornithinolytica on o'ahu: a case of community-acquired r. ornithinolytica urinary tract infection.human infection with raoultella ornithinolytica is rare, with only ten cases having been reported previously. this case report describes a local patient diagnosed with community-acquired r. ornithinolytica urinary tract infection in 2014.201526019987
catheter-related blood stream infection caused by raoultella ornithinolytica.raoultella spp. representatives are gram-negative capsulated, nonmotile rods. these bacteria are found in the natural environment: plants, water, soil and insects. r. ornithinolytica is one of the three species of raoultella. r. ornithinolytica is the only species within the genus which has the ability to produce ornithine decarboxylase. human infections related to r. ornithinolytica are exceedingly rare. the present case report describes catheter-related blood stream infection caused by r. orni ...201525894520
chemical composition and antibacterial activity of lavandula coronopifolia essential oil against antibiotic-resistant bacteria.the aim of this study was to analyse the composition of the essential oil (eo) of lavandula coronopifolia from morocco and to evaluate its in vitro antibacterial activity against antibiotic-resistant bacteria isolated from clinical infections. the antimicrobial activity was assessed by a broth micro-well dilution method using multiresistant clinical isolates of 11 pathogenic bacteria: klebsiella pneumoniae subsp. pneumoniae, klebsiella ornithinolytica, escherichia coli, enterobacter cloacae, ent ...201525174508
whole-cell-based identification of electrochemically active bacteria in microbial fuel cells by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.electrochemically active bacteria (eab) that are capable of producing electricity from renewable biomass and organic wastes have been of particular interest in recent years. methods for selective enrichment, accurate identification and easy acquisition of eab fingerprints for phylogenetic characterization would facilitate utilization of these bioenergy-producing species in practical environmental engineering applications.201526522312
molecular detection of the new delhi metallo-β-lactamase-1 gene in enterobacteriaceae isolates in a tertiary medical centre.new delhi metallo-β-lactamase-1 (ndm-1) is a relatively recent carbapenemase enzyme that inactivates all β-lactam antibiotics with the exception of aztreonam. this study aims to ascertain the baseline prevalence and antibiotic susceptibility patterns of ndm-1-producing enterobacteriaceae in a tertiary medical center in malaysia.201526712667
genetic, biochemical characterization and mutagenesis of the chromosomal class a β-lactamase of raoultella (formerly klebsiella) terrigena.chromosomal class a β-lactamases have been characterized in raoultella ornithinolytica and raoultella planticola. the purpose of this study was to characterize that of raoultella terrigena.201526092758
antimycobacterial action of a new glycolipid-peptide complex obtained from extracellular metabolites of raoultella ornithinolytica.in this paper, an antimycobacterial component of extracellular metabolites of a gut bacterium raoultella ornithinolytica from d. veneta earthworms was isolated and its antimycobacterial action was tested using mycobacterium smegmatis. after incubation with the complex obtained, formation of pores and furrows in cell walls was observed using microscopic techniques. the cells lost their shape, stuck together and formed clusters. surface-enhanced raman spectroscopy analysis showed that, after incub ...201526547373
new delhi metallo-β-lactamase producing extensively drug-resistant raoultella ornithinolytica isolated from drain fluid following whipple's pancreaticoduodenectomy. 201526858504
diabetic foot infection caused by raoultella ornithinolytica.the case of a diabetic foot infection caused by raoultella ornithinolytica is reported.201526637513
[postoperative osteomyelitis of a distal phalanx caused by raoultella ornithinolytica].we report on a case of osteomyelitis of a distal phalanx of the right ring finger of a 62-year-old patient, which occurred 11 months after transosseous-transungual refixation of a closed flexor digitorum profundus tendon avulsion caused by raoultella ornithinolytica. r. ornithinolytica is an encapsulated gram-negative aerobic bacillus. in the literature only 13 cases of human infection by r. ornithinolytica are mentioned. to the best of our knowledge, this is the first case of an osteomyelitis c ...201625988981
whole-genome sequence of a blaoxa-48-harboring raoultella ornithinolytica clinical isolate from lebanon.we analyzed the whole-genome sequence of ablaoxa-48-harboringraoultella ornithinolyticaclinical isolate from a patient in lebanon. the size of theraoultella ornithinolyticacmul058 genome was 5,622,862 bp, with a g+c content of 55.7%. we deciphered all the molecular mechanisms of antibiotic resistance, and we compared our genome to other availabler. ornithinolyticagenomes in genbank. the resistome consisted of 9 antibiotic resistance genes, including a plasmidicblaoxa-48gene whose genetic organiz ...201626833149
[sepsis caused by raoultella ornithinolytica in an immunocompetent patient].raoultella ornithinolytica is a species of gram-negative encapsulated and aerobic bacteria belonging to the family enterobacteriaceae and is mainly found in fish. the most distinctive feature of this bacterium is the ability to convert histidine to histamine; therefore, the consumption of decomposing fish infected by r. ornithinolytica causes rashes, diarrhea, flushing, sweating and vomiting. this food poisoning is also called histamine fish poisoning; however, human infections with r. ornithino ...201626811948
raoultella ornithinolytica: an unusual pathogen for prosthetic joint infection.we herein report the first case of a prosthetic joint infection caused by raoultella ornithinolytica in an immunocompetent patient. the clinical outcome was favorable after a two-stage prosthetic exchange and a six-month course of antimicrobial therapy.201627500082
septic arthritis of the knee due to raoultella ornithinolytica. 201627488819
bacteremia caused by raoultella ornithinolytica in two children.we encountered 2 immunocompromised children complicated by raoultella ornithinolytica bacteremia. one had received methylprednisolone pulse therapy for iga nephropathy, and the other had leukopenia because of chemotherapy for leukemia. both children had no specific symptoms, and r. ornithinolytica bacteremia was identified by routine blood culture. both patients were successfully treated with antibiotic treatment.201626974750
emerging role of raoultella ornithinolytica in human infections: a series of cases and review of the literature.raoultella ornithinolytica is known to inhabit aquatic environments. the clinical features and outcomes of human infections caused by r. ornithinolytica have been reported for only a limited number of cases.201626921549
high production of 2,3-butanediol (2,3-bd) by raoultella ornithinolytica b6 via optimizing fermentation conditions and overexpressing 2,3-bd synthesis genes.biological production of 2,3-butandiol (2,3-bd) has received great attention as an alternative to the petroleum-based 2,3-bd production. in this study, a high production of 2,3-bd in fed-batch fermentation was investigated with a newly isolated bacterium designated as raoultella ornithinolytica b6. the isolate produced 2,3-bd as the main product using hexoses (glucose, galactose, and fructose), pentose (xylose) and disaccharide (sucrose). the effects of temperature, ph-control schemes, and agita ...201627760200
bioprospecting of lipolytic microorganisms obtained from industrial effluents.the lipases have ability to catalyze diverse reactions and are important in different biotechnological applications. the aim of this work was to isolate and characterize microorganisms that produce lipases, from different food industry effluents localized in pelotas, rs/brazil. bacteria were identified using gram stain and biochemical tests (vitek 2(r)). fungi were identified according to macro and micromorphology characteristics. the extracellular lipase production was evaluated using the rhoda ...201627556331
characterization of escherichia coli and other enterobacteriaceae in producer-distributor bulk milk.the current study was undertaken to characterize escherichia coli and other enterobacteriaceae in raw and pasteurized producer-distributor bulk milk (pdbm). a total of 258 samples were collected from purchase points in 8 provinces in south africa. the samples were tested for antibiotic residues, phosphatase, total aerobic bacteria, coliforms, and e. coli counts. matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was used for identification of isolates. escherichia coli ...201627720154
a single-day point-prevalence study of faecal carriers in long-term care hospitals in madrid (spain) depicts a complex clonal and polyclonal dissemination of carbapenemase-producing enterobacteriaceae.the objective of this study was to describe the prevalence and microbiological characteristics of carbapenemase-producing enterobacteriaceae (cpe) colonizing patients in long-term care hospitals (ltchs) in madrid, spain.201626518051
bioremediation of polycyclic aromatic hydrocarbon (pah) compounds: (acenaphthene and fluorene) in water using indigenous bacterial species isolated from the diep and plankenburg rivers, western cape, south africa.this study was conducted to investigate the occurrence of pah degrading microorganisms in two river systems in the western cape, south africa and their ability to degrade two pah compounds: acenaphthene and fluorene. a total of 19 bacterial isolates were obtained from the diep and plankenburg rivers among which four were identified as acenaphthene and fluorene degrading isolates. in simulated batch scale experiments, the optimum temperature for efficient degradation of both compounds was determi ...201627956015
laurus nobilis, zingiber officinale and anethum graveolens essential oils: composition, antioxidant and antibacterial activities against bacteria isolated from fish and shellfish.several bacterial strains were isolated from wild and reared fish and shellfish. the identification of these strains showed the dominance of the aeromonas hydrophila species in all seafood samples, followed by staphylococcus spp., vibrio alginolyticus, enterobacter cloacae, klebsiella ornithinolytica, klebsiella oxytoca and serratia odorifera. the isolates were studied for their ability to produce exoenzymes and biofilms. the chemical composition of the essential oils from laurus nobilis leaves, ...201627782086
identification of bacterial contaminants in polyherbal medicines used for the treatment of tuberculosis in amatole district of the eastern cape province, south africa, using rapid 16s rrna technique.polyherbal medicines are used for the treatment of many diseases in many african and asian communities. with the increasing use of these remedies, several investigations have shown that they are associated with a broad variety of residues and contaminants. this study investigates the presence of bacteria in the polyherbal medicines used for the treatment of tuberculosis (tb) in the eastern cape province of south africa.201627549141
carbapenemase-producing enterobacteriaceae recovered from a spanish river ecosystem.the increasing resistance to carbapenems is an alarming threat in the fight against multiresistant bacteria. the dissemination properties of antimicrobial resistance genes are supported by their detection in a diverse population of bacteria, including strains isolated from the environment. the objective of this study was to investigate the presence of carbapenemase-producing enterobacteriaceae (cpe) collected from a river ecosystem in the barcelona metropolitan area (spain). identification of β- ...201728380016
screening for fecal carriage of mcr-producing enterobacteriaceae in healthy humans and primary care patients.the extent of the occurrence of the plasmid-encoded colistin resistance genes mcr-1 and mcr-2 among humans is currently sparsely studied in western europe.201728316780
occurrence of blakpc-2, blactx-m, and mcr-1 in enterobacteriaceae from well water in rural china.we report on the coexistence of mcr-1 and blactx-m in multidrug-resistant, extended-spectrum β-lactamase-producing escherichia coli belonging to the sequence type 10 complex isolated from well water in rural china. raoultella ornithinolytica with blakpc-2 was also detected in well water from the same area. this study shows that genes coding for resistance to last-resort antibiotics are present in wells in rural china, indicating a potential source of antibiotic resistance.201728115344
esbl-producing gram-negative organisms in the healthcare environment as a source of genetic material for resistance in human infections.the increasing prevalence of extended-spectrum β-lactamase (esbl)-producing enterobacteriaceae in the healthcare setting and in the community despite established infection control guidelines indicates that these microorganisms may possess survival strategies that allow them to persist in the environment.201727771149
sequencing and comparative genomics analysis of the inchi2 plasmids pt5282-mpha and p112298-cata and the inchi5 plasmid pynkp001-dfra.incompatibility group inchi plasmids are important vectors of antibiotic resistance in enterobacteriaceae. in this study, a scheme for typing inchi into five separately clustering subgroups, including previously designated inchi1-3 as well as inchi4-5, was proposed based on sequenced inchi plasmids. the complete nucleotide sequences of the inchi2 plasmids pt5282-mpha and p112298-cata and the inchi5 plasmid pynkp001-dfra from clinical enterobacter cloacae, citrobacter freundii and raoultella orni ...201728390961
septic arthritis of the temporomandibular joint caused by rare bacteria raoultella ornithinolytica.septic arthritis of the temporomandibular joint (tmj) is an unusual disease in adults. inoculation of the pathogen may occur through traumatic or iatrogenic injuries, or more often by haematogenous spread from a distant focus. the cause of infection is unknown in most cases. a case of ostensibly mild septic arthritis of the tmj with a good response to antibiotic therapy and lavage, but that finally led to fatal destruction of the joint structures in a 38-year-old female patient, is reported here ...201727726907
molecular characteristics and antibiotic resistance profiles of klebsiella isolates in mthatha, eastern cape province, south africa.the increase in the incidence of extended-spectrum β-lactamase- (esbl-) producing klebsiella species has become a serious problem worldwide, because of their incrimination in antibiotic resistance. the objective of this study is to investigate the resistance genes responsible for esbl-producing klebsiella species and carbapenemase-producing klebsiella (cre) isolated in mthatha and to study their epidemiology. a prospective, descriptive study of 202 nonrepetitive samples from patients was obtaine ...201728250772
high production of 2,3-butanediol from glycerol without 1,3-propanediol formation by raoultella ornithinolytica b6.conversion of crude glycerol derived from biodiesel processes to value-added chemicals has attracted much attention. herein, raoultella ornithinolytica b6 was investigated for the high production of 2,3-butanediol (2,3-bd) from glycerol without 1,3-propanediol (1,3-pd) formation, a by-product hindering 2,3-bd purification. by evaluating the effects of temperature, agitation speed, and ph control strategy, the fermentation conditions favoring 2,3-bd production were found to be 25 °c, 400 rpm, and ...201728078395
raoultella spp.-clinical significance, infections and susceptibility to antibiotics.the genus raoultella belongs to the family of enterobacteriaceae. raoultella spp. are gram-negative, aerobic, non-motile rods. this genus can be distinguished from the genus klebsiella, in that genus use histamine as the only source of carbon in the medium. also, raoultella grow at 4 °c and do not produce gas from lactose at 44.5 °c. raoultella sp. is known to inhabit natural environments (water, soil, plants). the reservoir of raoultella is the gastrointestinal tract and upper respiratory tract ...201728063019
metabolic engineering of raoultella ornithinolytica bf60 for production of 2,5-furandicarboxylic acid from 5-hydroxymethylfurfural.2,5-furandicarboxylic acid (fdca) is an important renewable biotechnological building block because it serves as an environmentally friendly substitute for terephthalic acid in the production of polyesters. currently, fdca is produced mainly via chemical oxidation, which can cause severe environmental pollution. in this study, we developed an environmentally friendly process for the production of fdca from 5-hydroxymethyl furfural (5-hmf) using a newly isolated strain, raoultella ornithinolytica ...201727795308
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