Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| ethanol extract of brassica rapa ssp. pekinensis suppresses tumor necrosis factor-α-induced inflammatory response in human umbilical vein endothelial cells. | brassica rapa l. ssp. pekinensis, commonly known as chinese cabbage, is a cruciferous vegetable traditionally consumed in east asia. although its habitual consumption could account for the low incidence of chronic vascular inflammation, the therapeutic and protective potential of phytochemicals derived from chinese cabbage has been poorly studied. in this study, we identified the phenolic compounds, kaempferol and quercetin, from the ethanol extract of chinese cabbage (etcc). we show for the fir ... | 0 | 28504909 |
| composite of pah-degrading endophytic bacteria reduces contamination and health risks caused by pahs in vegetables. | vegetables accumulate polycyclic aromatic hydrocarbons (pahs) at high concentrations when grown in contaminated sites. inoculation with pah-degrading endophytic bacteria (ebpah) has been recognized as one of the most promising ways to remove pahs from plant bodies; however, the performance of single endophytic bacteria is generally limited. this investigation used a composite of eight ebpah to reduce the contamination and health risk posed by 16 epa priority pahs in vegetables including chinese ... | 0 | 28448936 |
| evaluation of pesticide residue dynamics in chinese cabbage, head cabbage and cauliflower. | pesticide residues from the time of application until harvest were analysed for 20, 17 and 18 active insecticidal and fungicidal substances in chinese cabbage, head cabbage and cauliflower, respectively. in total, 40 mathematical models of residue degradation were developed using a first-order kinetic equation, and from these models it was possible to forecast the action pre-harvest interval for a given action threshold for low-residue production in brassica vegetables as a percentage of the max ... | 0 | 28349738 |
| study of the genotoxicity of organic extracts from wastewater-irrigated vegetables using in vitro and in vivo biological tests. | the purpose of this study was to explore genotoxicity due to organic pollutants in wastewater-irrigated vegetables using biological and chemical analyses. chinese cabbages from wastewater-irrigated farmland were taken as the research object. for the in vitro test, dna damage was characterized in rat hepatocytes exposed to organic extracts from the cabbages using the comet assay. for the in vivo tests, mice were exposed to organic extracts from the cabbages. dna damage was assessed in mouse perip ... | 0 | 28092007 |
| simulating the transfer of strontium-90 from soil to leafy vegetables by using strontium-88. | the transfer, from soil to chinese cabbage and spinach, of radioactive strontium-90 released as a result of accidents in nuclear power stations was studied using a stable isotope of strontium, namely nuclide strontium-88 ((88)sr). the study led to an experimental model for assessing the hazard of radionuclide strontium-90 ((90)sr) entering the food chain and for predicting the risk to food safety. chinese cabbage and spinach were grown in pots in a greenhouse and irrigated with deionized water c ... | 0 | 27829691 |
| the accumulation and health risk of heavy metals in vegetables around a zinc smelter in northeastern china. | mining and smelting activities engender soil contamination by metals severely. a field survey was conducted to investigate the present situation and health risk of heavy metals (cd, pb, zn, cu, cr, as, and hg) in soils and vegetables in the surrounding area of an 80-year-old zinc smelter in northeastern china. soil ph, organic matter (som), and cation exchange capacity (cec) were determined, and their relations with heavy metal contents in edible parts of vegetables were analyzed. results showed ... | 0 | 27679998 |
| a field study on the dynamic uptake and transfer of heavy metals in chinese cabbage and radish in weak alkaline soils. | vegetables and crops can take up heavy metals when grown on polluted lands. the concentrations and dynamic uptake of heavy metals vary at different growth points for different vegetables. in order to assess the safe consumption of vegetables in weak alkaline farmlands, chinese cabbage and radish were planted on the farmlands of baiyin (polluted site) and liujiaxia (relatively unpolluted site). firstly, the growth processes of two vegetables were recorded. the growth curves of the two vegetables ... | 0 | 27473619 |
| sorption equilibrium of emerging and traditional organic contaminants in leafy rape, chinese mustard, lettuce and chinese cabbage. | emerging and petroleum contaminants could transfer into food chains by plant uptake, potentially causing food security problems. to build a prediction model, the sorption equilibrium and uptake kinetics of toluene, p-xylene, naphthalene, bisphenol a, and 4-bromo-diphenyl ether in some common leafy vegetables including leafy rape, chinese mustard, lettuce and chinese cabbage were examined. the kinetic experiments revealed that high sorption rates were observed for these plants that had high lipid ... | 0 | 27085315 |
| the green peach aphid myzus persicae perform better on pre-infested chinese cabbage brassica pekinensis by enhancing host plant nutritional quality. | the green peach aphid, myzus persicae sulzer, is a notorious pest on vegetables, which often aggregates in high densities on crop leaves. in this study, we investigated whether m. persicae could suppress the resistance level of chinese cabbage brassica pekinensis. m. persicae performed better in terms of weight gain (~33% increase) and population growth (~110% increase) when feeding on previously infested (pre-infested) chinese cabbage compared with those on non-infested plants. however, when gi ... | 0 | 26905564 |
| vegetarian diet and reduced uterine fibroids risk: a case-control study in nanjing, china. | the aim of this study was to investigate whether a vegetarian diet correlates with a potential reduced risk of uterine fibroids. | 0 | 26458740 |
| temperature determines size and direction of effects of elevated co2 and nitrogen form on yield quantity and quality of chinese cabbage. | rising atmospheric co2 concentrations (e[co2 ]) are presumed to have a significant impact on plant growth and yield and also on mineral nutrient composition, and therefore, on nutritional quality of crops and vegetables. to assess the relevance of these effects in future agroecosystems it is important to understand how e[co2 ] interacts with other environmental factors. in the present study, we examined the interactive effects of e[co2 ] with temperature and the form in which nitrogen is supplie ... | 0 | 26390257 |
| relations of fine-root morphology on (137)cs uptake by fourteen brassica species. | fourteen brassica species consisting of seven leafy vegetables and seven root vegetables were examined for (137)cs uptake differences in relation to their fine-root morphological characters. a pot experiment was conducted from november 2014 to february 2015 in a phytroton using a contaminated soil of fukushima prefecture. leafy vegetables showed bigger root diameters, larger root surface area and larger root volume. consequently, leafy vegetables had higher (137)cs uptake compared to root vegeta ... | 0 | 26355648 |
| cadmium contamination of soil and crops is affected by intercropping and rotation systems in the lower reaches of the minjiang river in south-western china. | cadmium (cd) accumulation and pollution in arable soils are particularly serious in the lower reaches of the minjiang river in southwest of china. in this study, the remediation efficiency of cd contamination in arable soils, the distribution pattern of cd concentration in crops, and the food safety to humans of three typical cropping systems (s1: maize + sweet potato-chinese cabbage, s2: maize + ginger-stem mustard, and s3: rice) were investigated and evaluated. after 1-year rotation, the perce ... | 0 | 26323960 |
| in vitro assessment of cadmium bioavailability in chinese cabbage grown on different soils and its toxic effects on human health. | the minimum concentration of cadmium (cd), by chinese cabbage grown on cd contaminated soils that can initiate toxicity in human liver cells using in vitro digestion coupled with caco-2/hl-7702 cell models was studied. cadmium bioaccessibility in the gastric phase for yellow soil (ys) cabbage (40.84%) and calcareous soil (cs) cabbage (21.54%) was significantly higher than small intestinal phase with the corresponding values of 21.2% and 11.11%, respectively. cadmium bioavailability was higher in ... | 0 | 26167479 |
| root growth and nitrate-nitrogen leaching of catch crops following spring wheat. | growing nitrogen (n) catch crops can reduce no(3)-n leaching after cultivating cereals. the objective of this study was to relate no(3)-n leaching to variation in the uptake of n and the size and distribution of the root systems of different catch crops species. in a 3-yr lysimeter experiment, phacelia (phacelia tanacetifolia benth.), sunflower (helianthus annuus l.), and a brassica species (yellow mustard [brassica alba l.] or a hybrid of turnip rape [b. rapa l. spp. oleifera (dc.) metzg.] and ... | 0 | 20400580 |
| cultivar-specific changes in primary and secondary metabolites in pak choi (brassica rapa, chinensis group) by methyl jasmonate. | glucosinolates, their hydrolysis products and primary metabolites were analyzed in five pak choi cultivars to determine the effect of methyl jasmonate (meja) on metabolite flux from primary metabolites to glucosinolates and their hydrolysis products. among detected glucosinolates (total 14 glucosinolates; 9 aliphatic, 4 indole and 1 aromatic glucosinolates), indole glucosinolate concentrations (153-229%) and their hydrolysis products increased with meja treatment. changes in the total isothiocya ... | 0 | 28481284 |
| ribosomes and polyribosomes in brassica pekinensis. | 1964 | 14240647 | |
| electron microscopic and biochemical characterization of fraction i protein. | electron micrographbs of high resolution have been obtained from fraction i protein of chinese cabbage leaves. the protein, which has ribulose-1,5-diphosphate carboxylase activity, appear to be a cube swth edge of about 120 angstroms. substrtucture canl be seen in individual particles, conlsistent with a model having 24 subunits, the number prescribed by the available physical and chlemical data. | 1965 | 5866657 |
| virus infection and photosynthesis. 1. increased photophosphorylation by chloroplasts from chinese cabbage infected with turnip yellow mosaic virus. | 1965 | 5850878 | |
| cytological changes induced by turnip yellow mosaic virus in chinese cabbage leaves. | 1966 | 5938885 | |
| electron microscopy of leaves infected with sowbane mosaic virus and other small polyhedral viruses. | leaves of chenopodium amaranticolor were infected with sowbane mosaic virus (smv), sectioned and examined by electron microscopy. leaves of brassica pekinensis and c. amaranticolor were infected with turnip yellow mosaic virus and cowpea mosaic virus, respectively, and similarly studied. with all three viruses it was difficult, in sections, to distinguish the small isometric virus particles from ribosomes though sometimes this was possible, especially when the viruses crystallized. pretreatment ... | 1967 | 18614059 |
| properties of ribulose-1,5-diphosphate carboxylase (carboxydismutase) from chinese cabbage and photosynthetic microorganisms. | ribulose-1,5-diphosphate carboxylase (carboxydismutase) was prepared from chinese cabbage [brassica petsai (parl)] and the k(m) values and molecular weight were determined. these parameters were found to be in good agreement with values reported for this enzyme from other higher plants. investigation of carboxydismutase activity from the photosynthetic micro-organisms chlamydomonas reinhardi (iu 89+), plectonema boryanum (iu 594), and chromatium strain d showed striking similarity to the higher ... | 1968 | 5700019 |
| ribonuclease isozymes in chinese cabbage systemically infected with turnip yellow mosaic virus. | 1968 | 5723669 | |
| some effects of kinetin on floated chinese cabbage leaf discs. | 1969 | 5792855 | |
| kinetin and carbohydrate metabolism in chinese cabbage. | the effects of kinetin on starch and sugar levels and on (14)co(2) and (32)p-orthophosphate labeling patterns of floated chinese cabbage (brassica pekinensis) leaf discs were investigated. kinetin caused gross starch degradation. neutral sugars were depressed by 30 to 40% in leaf tissue treated with kinetin for 24 hours. (14)co(2) labeling of leaf discs pretreated with kinetin for 24 hours showed increased radioactivity in chloroform-soluble material and most sugar phosphates, and a 35 to 40% de ... | 1971 | 16657661 |
| rna-dependent rna polymerase in chinese cabbage. | 1971 | 5572618 | |
| non-uniformities in the metabolism of excised leaves and leaf discs. | in intact tobacco and chinese-cabbage (brassica pekinensis) leaves an "ageing" process begins as soon as the leaves are excised. the terminal few millimetres of the petiole increasingly preempt materials such as phosphate and uracil taken up by the leaf. actinomycin d treatment blocks this process and leads to increased uptake of such materials into the lamina.immediately after excision there is a radial "geographical" gradient, in the ability of excised leaf discs to accumulate phosphate and ur ... | 1971 | 24487445 |
| crystallization of turnip yellow mosaic virus in wilted leaves of chinese cabbage by treatment at high temperature. | 1971 | 5315697 | |
| incidence of parasites found on vegetables collected from markets and vegetable gardens in taegu area. | a parasitic survey on vegetables collected from markets and vegetable gardens in taegu area was conducted for the discovery of human parasitic eggs and larvae. three species of vegetable, lettuce (lactuca sativa), young radish(raphanus sapivus) and chinese cabbage (brassica pekinensis) were selected. all vegetable leaves were washed with a hard brush, and then species of parasites and the approximate mean number of parasitic eggs per 200 grams of vegetable leaves were investigated. when vegetabl ... | 1972 | 12913510 |
| evidence for modification of protein phosphorylation by cytokinins. | kinetin stimulated phosphorylation of protein in floated chinese-cabbage leaf discs, but inhibited protein phosphorylation in nuclei+chloroplast extracts from chinese-cabbage or tobacco leaves. kinetin also inhibited protein phosphorylation in isolated tobacco nuclei or nuclei from carrot secondary-phloem tissue. purified chinese-cabbage leaf ribosomes exhibited protein kinase activity which was inhibited by kinetin and zeatin. the ribosome-associated kinase responded to kinetin and zeatin diffe ... | 1972 | 4348261 |
| leaf ontogeny and virus replication in brassica pekinensis infected with turnip yellow mosaic virus. | 1972 | 4623503 | |
| some ultrastructural and cytochemical observations on chinese cabbage leaves infected with cauliflower mosaic virus. | 1972 | 4111055 | |
| fine structure of vesicles induced in chloroplasts of chinese cabbage leaves by infection with turnip yellow mosaic virus. | 1973 | 4584930 | |
| the sequence of early cytological changes in chinese cabbage leaf cells following systemic infection with turnip yellow mosaic virus. | 1974 | 4833533 | |
| evidence for tymv-induced rna and dna synthesis in the nuclear fraction from infected chinese cabbage leaves. | 1974 | 4820885 | |
| studies on environmental contamination by uranium. 4. uranium absorption by chinese cabbage. | 1974 | 4842199 | |
| a chlorophyll-protein complex lacking in photosystem i mutants of chlamydomonas reinhardtii. | sodium dodecyl sulfate gel electrophoresis of unheated, detergent-solubilized thylakoid membranes of chlamydomonas reinhardtii gives two chlorophyll-protein complexes. chlorophyll-protein complex i (cp i) is the blue-green in color and can be dissociated by heat into "free" chlorophyll and a constituent polypeptide (polypeptide 2; mol wt 66,000). similar experiments with spinach and chinese cabbage show that the higher plant cp i contains an equivalent polypeptide but of slightly lower molecular ... | 1975 | 1194353 |
| [in vitro infection of chinese cabbage protoplasts by the turnip yellow mosaic virus]. | 1975 | 808296 | |
| inhibition of turnip yellow mosaic virus synthesis by pyrimidine analogues. | the pyrimidine analogues 2-thiouracil, 2-thiouridine, 6-azauracil and 6-azauridine all inhibited the synthesis of turnip yellow mosaic virus (tymv) and increased the synthesis of empty virus protein shells in infected chinese cabbage leaf discs. uracil and uridine reversed these effects. 2-thiouracil also reduced the utp pool in tymv infected leaf discs. the results are consistent with the suggestion that these analogues or their in vivo derivatives affect virus synthesis by inhibiting the biosy ... | 1976 | 989025 |
| herbicidins a and b, two new antibiotics with herbicidal activity. i. producing organism and biological activities. | herbicidins a and b, two new antibiotics with selective and contact herbicidal activity, were produced by a new species of streptomyces designated as s. sagononensis. among tested microorganisms, herbicidins indicated some activity against fungi in vitro and xanthomonas oryzae in vivo. their characteristic features were brought into relief by their selective and contact killing effect on many dicotyledonous plant as well as their inhibition of germination of the plant seeds, such as chinese cabb ... | 1976 | 993127 |
| location, rate and asymmetry of ds-rna synthesis during replication of tymv in chinese cabbage. | the ds-rna induced by tymv infection in the nuclei of infected chinese cabbage leaf cells became labelled with [32p]phosphate most rapidly before production of virus could be detected. in contrast, the ds-rna associated with the chloroplasts became labelled most rapidly during the period of maximum virus production. | 1976 | 1275406 |
| effects of kinetin on phosphorylation of leaf membrane proteins. | isolated chinese cabbage leaf membranes were phosphorylated by membrane-associated protein kinase(s) in the presence or [gamma-32p]atp. membrane-associated 32p radioactivity appeared to be bound to membrane proteins. both smooth cell membranes and chloroplast lamellae reacted with atp. phosphorylation of the membranes was inhibited by ca2+ and partially inhibited by kinetin or 6-benzyladenine. the possibility that cytokinin effects on membrane phosphorylation might increase ion availability was ... | 1976 | 1252471 |
| proceedings: autoradiographical studies on rna synthesis in healthy and tymv infected chinese cabbage: effect of actinomycin d. | 1976 | 1275409 | |
| proceedings: biochemical studies on rna synthesis in healthy and tymv infected chinese cabbage: effect of actinomycin d. | 1976 | 1275408 | |
| proceedings: replication of tymv in chinese cabbage protoplasts. | 1976 | 1275407 | |
| [the effect of actinomycin d on the production of viruses by the protoplasts of chinese cabbage infected in vitro by the yellow mosaic virus of turnips]. | chinese cabbage (brassica sinensis l. var. cantonner) protoplasts were infected by turnip yellow mosaic virus (tymv) and inoculated in the presence or absence of actinomycin d. virus production was determined 40 hrs. after inoculation, the time required for the virus replication cycle to be terminated. while actinomycin d had no effect on tymv production when present at a concentration of 1 microng/ml, a 50 to 80% inhibition of virus production was noticed at concentrations of the order of 5 to ... | 1977 | 405160 |
| isolation of viruses by electro-extraction of infected plants. | the isolation of viruses from infected plant material by a process termed electro-extraction appeared to be a convenient and simple method of obtaining viruses in a fair state of purity. the method has the advantage over the conventional methods of virus purification that the infected plant tissue is not disintegrated and that organic solvents such as chloroform and butanol are avoided. the procedure used was demonstrated on the extraction of tobacco mosaic virus (tmv) from infected tobacco and ... | 1977 | 909872 |
| spectral characterization of five chlorophyll-protein complexes. | sodium dodecyl sulfate-solubilized chloroplast internal membranes of higher plants (cowpea [vigna unguiculata l. walp], chinese cabbage [brassica chinensi l.], and tobacco [nicotiana tabacum l.]) are resolved by polyacrylamide gel electrophoresis into two chlorophyll a- and three chlorophyll a,b-proteins. a small portion (about 15%) of the membrane chlorophyll migrates as a component of high electrophoretic mobility and presumably consists of detergent-complexed, protein-free pigment.one of the ... | 1978 | 16660625 |
| herbicidins c and e, two new necleoside antibiotics. | streptomyces saganonensis no. 4075 used for production of herbicidins a and b has also produced other two antibiotics, herbicidins c and e in submerged culture. isolation of the antibiotics was performed by adsorption on resinous adsorbent followed by elution with aqueous meoh and completed by silica-gel chromatography. physico-chemical characteriation revealed that herbicidins c and e are new nucleoside antibiotics having an adenine moiety in their structures. herbicidins c and e indicated inhi ... | 1979 | 117003 |
| populations of the spinach wilt pathogen, fusarium oxysporum f. sp. spinaciae, in the root tissues, rhizosphere, and soil in the field. | populations of fusarium oxysporum f. sp. spinaciae in root tissues and rhizosphere soil of diseased spinach plants were higher than in the root tissues and rhizosphere soil of healthy plants. populations in soil rhizosphere were higher than in nonrhizosphere soil. the fungus populations were very low in the root tissues of the nonsusceptible strawberry, broccoli, chinese cabbage, and mustard grown in the infested field. the populations were low at the beginning of the season, increased, and rema ... | 1979 | 436018 |
| synthesis and accumulation of polyamines and s-adenosylmethionine in chinese cabbage infected by turnip yellow mosaic virus. | 1979 | 465028 | |
| structure of plant nuclear and ribosomal dna containing chromatin. | digestion of plant chromatin from brassica pekinensis and matthiola incana with staphylococcus nuclease leads to a dna repeat of 175 plus or minus 8 and a core size of 140 base pairs. dnase i digestion results in multiples of 10 bases. ribosomal rnn genes were studied as a model system for active plant chromatin because of their great redundancy and their high transcriptional activity in growing and differentiating tissues. the actively transcribed genes were identified by nascent rna of ribosom ... | 1979 | 514813 |
| effect on growth and nickel content of cabbage plants watered with nickel solutions. | chinese cabbage plants were watered with different concentrations of nicl2 solutions and the effect on growth and uptake of nickel in the plants were studied. no toxic effect on plant growth was observed. a higher content of nickel was found in the plants exposed to more concentrated nickel solutions. nickel contamination and its clinical consequences are discussed. | 1979 | 498768 |
| determination of residues of fensulfothion and its metabolites in chinese cabbage, japanese radish , and turnips. | a method was developed using a selective nitrogen-phosphorus detector to determine the combined total residues, as the oxygen analogs, of fensulfothion (o,o-diethyl o-[p-(methylsulfinyl)-phenyl] phosphorothioate) and its 3 metabolites. the procedure was used to determine harvest residues in chinese cabbage, japanese radish, and turnips treated with 2 application rates of fensulfothion. residues found after application of 2 oz active ingredient (ai)/100 lineal ft of row ranged from 0.026 ppm for ... | 1980 | 6447687 |
| infection of chlorophyll-less protoplasts from etiolated chinese cabbage hypocotyls by turnip yellow mosaic virus. | protoplasts were prepared from hypocotyls of dark-grown, 8-day-old chinese cabbage seedlings. pectinase and cellulase were used together in a one-step procedure. these hypocotyl protoplasts did not contain detectable amounts of chlorophyll. they could be infected in vitro with turnip yellow mosaic virus (tymv). virus replication was demonstrated by direct immunofluorescence, enzyme-linked immunosorbent assay, virus recovery by sucrose density gradient centrifugation, and infectivity tests on chi ... | 1980 | 18631665 |
| timing of the synthesis of empty shells and minor nucleoproteins in relation to turnip yellow mosaic virus synthesis in brassica protoplasts. | following inoculation of chinese cabbage protoplasts in vitro with turnip yellow mosaic virus, the development of characteristic changes in the chloroplasts was significantly asynchronous when observed by light or electron microscopy. in fully infected protoplasts there were only about 25% as many peripheral vesicles (the site of virus rna synthesis) as in infected cells in the intact leaf. the yield of virus per infected protoplast was only about 1/10 th that found for an equivalent population ... | 1981 | 18635063 |
| the synthesis of polyamines from methionine in intact and disrupted leaf protoplasts of virus-infected chinese cabbage. | in exploring the role of the chloroplast in the multiplication of turnip yellow mosaic virus, the biosyntheses of the major viral polyamine, spermidine, as well as that of the tetramine, spermine were studied. the synthesis of these polyamines from [2-(14)c]methionine in protoplasts of chinese cabbage leaf cells derived from healthy plants or those infected by turnip yellow mosaic virus were examined. populations of protoplasts of infected leaves are homogeneous with respect to containing chloro ... | 1981 | 16662066 |
| turnip yellow mosaic virus rna is aminoacylated in vivo in chinese cabbage leaves. | turnip yellow mosaic virus (tymv) contains a trna-like structure as an integral part of its genome. this structure is located at the extreme 3' end of the viral rna and is the acceptor of valine after 3'-terminal adenylation. it is known that in vitro (with bacterial, yeast, or plant systems) and in vivo (upon microinjection into xenopus laevis oocytes) a series of trna-specific enzymes can recognize this structure in the viral rna. we report that tymv rna is valylated and consequently adenylate ... | 1982 | 16453426 |
| length requirements for trna-specific enzymes and cleavage specificity at the 3' end of turnip yellow mosaic virus rna. | this paper describes the minimum length of the turnip yellow mosaic virus (tymv) rna necessary to fulfill the trna-like properties of the viral rna: 50 to 75 nucleotides and 86 nucleotides from the 3' end of tymv rna are sufficient for adenylation and valylation respectively by the escherichia coli system. the size of the trna-like fragments obtained in vitro in the presence of an e. coli, a reticulocyte or a chinese cabbage leaf extract has also been determined. among the major fragments libera ... | 1982 | 6176943 |
| [change in incidence of parasite eggs and larvae from vegetable in the markets of taegu] | a survey on vegetables, which consisted of lettuce (lactuca sativa), young radish (raphanus sapivus), and chinese cabbage (brassica pekinensis) collected from 6 markets in the taegu city, was conducted for the discovery of helminth eggs and larvae, from july 1982 to june 1983. the results were compared with the data obtained from vegetables collected at the same markets by lee (1969) and choi and lee (1972). both sides of vegetable leaves were washed carefully with a hard brush, and the species ... | 1983 | 12902676 |
| detection of partially proteolysed cauliflower mosaic virus coat protein in infected leaf tissue by western blotting. | cauliflower mosaic virus (camv) capsid polypeptides were detected by immunoelectroblotting ('western blotting') 10-16 days after infection of chinese cabbage leaves. the predominant polypeptides detected had molecular weights of 42,000 and 37,000 suggesting that in vivo proteolysis of the 55,000-58,000 molecular weight coat protein had taken place. the use of laboratory-made nitrocellulose membranes for western blotting is reported. phosphate-sds buffer was more suitable than tris-glycine buffer ... | 1983 | 6352722 |
| [hydroxycinnamic acid esters in brassicaceous vegetables and garden cress]. | the esters of caffeic, p-coumaric and ferulic acid with glucose or quinic acid and sinapoylglucose were determined in cauliflower, kale, brussel sprouts, red, white, savoy and chinese cabbage and in garden cress by two different hplc methods with gradient elution. in addition the occurrence of sinapin (sinapoylcholin) was checked. the pattern of hydroxycinamic acid esters in the brassica species varied appreciably. in garden cress besides sinapin only sinapoylglucose was found. sinapoylglucose i ... | 1983 | 6613360 |
| turnip yellow mosaic virus rna-replicase contains host and virus-encoded subunits. | the enzyme (rna-replicase) involved in the synthesis of viral rna has been purified from turnip yellow mosaic virus (tymv)-infected chinese cabbage leaves. the rna-replicaset contains two major subunits: one of apparent molecular weight 115,000 (115k) and the other of 45k. we have raised antisera against the purified tymv-rna-replicase and have demonstrated by immunoaffinity chromatography and immunoblotting that the 115k polypeptide is coded by the viral rna but that the 45k protein is of host ... | 1984 | 18639811 |
| subcellular localization of spermidine synthase in the protoplasts of chinese cabbage leaves. | previous studies on the presence of spermidine synthase (ec 2.5.1.16) in the protoplasts of chinese cabbage (brassica pekinensis var pak choy) leaves had detected a small but significant fraction of the enzyme in a crude chloroplast fraction (cohen, balint, sindhu 1981 plant physiol 68: 1150-1155). to establish whether this enzyme is truly a chloroplast component, we have isolated purified intact chloroplasts from protoplasts by density gradient centrifugation in silica sols (ludox am). such chl ... | 1984 | 16663802 |
| propylamine transferases in chinese cabbage leaves. | we have found spermidine synthase and spermine synthase activities in extracts of leaves of chinese cabbage (brassica pekinensis var. pak choy) and have developed an assay of the former in crude extracts. the method is based on the transfer of the propylamine moiety of decarboxylated s-adenosylmethionine to labeled putrescine, followed by ion-exchange separation of the labeled amine substrate and product, which are then converted to the 5-dimethylamino-1-napthalene sulfonyl (dansyl) derivatives ... | 1984 | 16663475 |
| analysis and occurrence of total n-nitroso compounds in the japanese diet. | a quantitative technique for differentiating the total n-nitrosamides from the total n-nitroso compounds has been devised. the principle of this method is based on the chemiluminescence response of a nitroso compound under different denitrosating conditions. we also examined the effect of nitrite scavengers on the stability of n-nitroso compounds and found that both ammonium sulfamate and hydrazine sulfate can be satisfactorily used for the detection of chemiluminescence response with hydrogen b ... | 1984 | 6533016 |
| a review of the structure and organization of the mitochondrial genome of higher plants. | the structure, organisation and functions of the mitochondrial genome of most groups of eukaryotic organisms are known to varying degrees with the notable exception of higher plants. with the recent completion of physical mapping studies on the brassica campestris (chinese cabbage, turnip) and zea mays (maize) mitochondrial genomes, many of the apparent problems of plant mitochondrial genome structure can now be answered. in this manuscript i review the literature relating to the physical observ ... | 1984 | 24310431 |
| s-adenosylmethionine decarboxylase and spermidine synthase from chinese cabbage. | the enzyme, s-adenosylmethionine (sam) decarboxylase (ec 4.1.1.50), has been demonstrated in leaves of chinese cabbage, (brassica pekinensis var pak choy). all of the enzyme can be found in extracts of the protoplasts obtained from the leaves of growing healthy or virus-infected cabbage. the protein has been purified approximately 1500-fold in several steps involving ammonium sulfate precipitation, affinity chromatography, and sephacryl s-300 filtration. the reaction catalyzed by the purified en ... | 1985 | 16664325 |
| dicyclohexylamine-induced shift of biosynthesis from spermidine to spermine in plant protoplasts. | an improved analytical method, based on high pressure liquid chromatography, has been developed for the simultaneous determination of the polyamines and s-adenosyl-containing compounds in extracts of plant protoplasts. the method involves simple procedures for sample preparation and permits quantification of 1 picomole or less for all the compounds. this method has been used to study the effects of dicyclohexylamine, an inhibitor of plant spermidine synthase (sindhu, r. k., s. s. cohen 1984 plan ... | 1985 | 16664284 |
| a mutagen precursor in chinese cabbage, indole-3-acetonitrile, which becomes mutagenic on nitrite treatment. | after treatment with nitrite, chinese cabbage showed direct-acting mutagenicity on salmonella typhimurium ta100 inducing 3100 revertants per g. one of the mutagen precursors that became mutagenic after nitrite treatment was isolated, and identified as indole-3-acetonitrile. after treatment with nitrite, 1 mg of indole-3-acetonitrile induced 17 400 revertants of ta100 and 21 000 revertants of ta98 without s9 mix. | 1985 | 3889600 |
| nitrosatable precursors of mutagens in vegetables and soy sauce. | nitrosatable precursors of mutagens that show mutagenicity to salmonella typhimurium ta100 without s9 mix after treatment with nitrite at ph 3 were found in various foods. from chinese cabbage, three indole compounds, indole-3-acetonitrile, 4-methoxyindole-3-acetonitrile, and 4-methoxyindole-3-aldehyde, were identified as mutagen precursors. 1-methylindole and 2-methylindole, which are present in cigarette smoke showed strong mutagen precursor activity. escherichia coli wp2 uvra/pkm101 is more s ... | 1985 | 3916206 |
| the incorporation of radiolabeled polyamines and methionine into turnip yellow mosaic virus in protoplasts from infected plants. | turnip yellow mosaic virus contains large amounts of nonexchangeable spermidine and induces an accumulation of spermidine in infected chinese cabbage. by 7 days after inoculation, a majority of protoplasts isolated from newly emerging leaves stain with fluorescent antibody to the virus. these protoplasts contain 1-2 x 10(6) virions per cell and continue to produce virus in culture for at least 48 hr. [14c]spermidine (10 microm) was taken up by these cells in amounts comparable to the original en ... | 1985 | 4060587 |
| the effects of dicyclohexylamine on polyamine biosynthesis and incorporation into turnip yellow mosaic virus in chinese cabbage protoplasts infected in vitro. | we have reported (r. balint and s. s. cohen, 1985, virology 144, 181-193) that protoplasts from plants infected with turnip yellow mosaic virus (tymv) continue to produce virus in culture and that newly formed virus particles contained predominantly newly synthesized spermidine and spermine. inhibition of spermidine synthesis by dicyclohexylamine (dcha), however, led to incorporation of preexisting spermidine and increased amounts of spermine into newly formed virions. we now report similar resu ... | 1985 | 4060588 |
| 4-methoxyindole derivatives as nitrosable precursors of mutagens in chinese cabbage. | two indole compounds isolated from fresh chinese cabbage were shown to be mutagen precursors that yielded direct-acting mutagens on treatment with nitrite. they were identified as 4-methoxyindole-3-acetonitrile and 4-methoxy-indole-3-aldehyde. when these compounds were treated with 50 mm nitrite at ph 3.0 for 1 h at 37 degrees c, 4-methoxyindole-3-acetonitrile induced 31,800 and 10,000 revertants of salmonella typhimurium ta100 and ta98, respectively, per mg of mutagen precursor in the absence o ... | 1986 | 3331681 |
| interaction of turnip yellow mosaic virus val-rna with eukaryotic elongation factor ef-1 [alpha]. search for a function. | the 3'-terminal trna-like structure in turnip yellow mosaic virus (tymv) rna can be adenylated by trna nucleotidyltransferase and subsequently aminoacylated by valyl-trna synthetase. here we present evidence that tymv val-rna can form a stable complex with eukaryotic wheat germ elongation factor ef-1alpha and gtp: the val-rna is protected by ef-1alpha.. gtp against digestion by rnase a. by affinity chromatography of tymv val-rna fragments on immobilized ef-1alpha . gtp, it has been established t ... | 1986 | 11681331 |
| characterization of the virus encoded subunit of turnip yellow mosaic virus rna replicase. | an antiserum raised against tymv-rna encoded protein p115 partially inhibits tymv rna replicase activity, demonstrating that this protein is involved in tymv rna synthesis. the detection of protein p115 by an antibody linked polymerase assay demonstrates that protein p115 is indeed a subunit of the tymv rna replicase, the enzyme known to synthesize viral rna in infected chinese cabbage. the use of translation products of other tymoviruses indicates that the serological relationship between the v ... | 1986 | 18640642 |
| mutagenic compounds in soy sauce, chinese cabbage, coffee and herbal teas. | 1986 | 3952098 | |
| recently identified nitrite-reactive compounds in food: occurrence and biological properties of the nitrosated products. | various japanese foodstuffs are directly-acting mutagens in salmonella typhimurium ta100 after nitrite treatment. such mutagen precursors include tyramine and beta-carboline derivatives, isolated from soya sauce, and indole-3-acetonitrile, 4-methoxyindole-3-acetonitrile and 4-methoxyindole-3-aldehyde, isolated from fresh chinese cabbage. a mutagen produced from tyramine with nitrite was found to be 4-(2-aminoethyl)-6-diazo-2,4-cyclohexadienone (3-diazotyramine), and one produced from indole-3-ac ... | 1987 | 3679387 |
| immunochemical properties of elongation factors 1 of plant origin. | elongation factors 1 (ef-1) have been isolated from different plants: wheat, yellow lupine, blue lupine, chinese cabbage and norway maple. antibodies for ef-1 from yellow lupine have been obtained in rabbits; antibodies for wheat ef-1 were elicited in mice. the immunological properties of ef-1 were assayed by the following methods: western blotting, double immunodiffusion and rocket immunoelectrophoresis. our results suggest that one antigenic site is similar for all plant elongation binding fac ... | 1988 | 3123226 |
| residues of dacthal and metabolites in field-treated chinese cabbage. | 1988 | 3233362 | |
| diet and cancer of the stomach: a case-control study in china. | a case-control study focusing on the role of single food items was undertaken in the heilongjiang area, 241 patients with histologically verified stomach cancer and an equal number of controls (with non-neoplastic diseases) matched by age, sex and area of residence being interviewed during a 2-year period. questions asked covered economic status, occupation, histories of smoking and drinking, diet and dietary habits and disease history. data concerning the average frequency and quantity of consu ... | 1988 | 3346096 |
| [multiple stepwise regression analysis of etiological factors of esophageal cancer in cixian county]. | cixian county, one of the high-risk counties of esophageal cancer in the world, has a standardized mortality of 142.19/10(5) population, 1969-1971. the incidence of esophageal cancer had dropped year by year from 1974 to 1982. the significance of the incidence tendency was studied. the results are highly significant (p less than 0.001). the causative factors of esophageal cancer including five independent variables: x1 (number of people taking sanitized water), x2 (number of people on pickled ch ... | 1989 | 2789130 |
| [preliminary observation on feeding preference of oncomelania for sedum sarmentosum]. | as a result of screening, we discovered that sedum sarmentosum bunge was most preferred among 317 kinds of snail food. both adult and young snails readily eat s. sarmentosum as evidenced by laboratory observation and dissection of the snails. in aquarium, more snails were attracted by s. sarmentosum than by chinese cabbage and chlorella suspension. on water surface, s. sarmentosum curtains attracted adult snails 2.1-3.7 times and immature snails 2.3-4.1 times more than rice straw curtains. | 1989 | 2591039 |
| plant regeneration from isolated microspore cultures of chinese cabbage (brassica campestris spp. pekinensis). | isolated microspores of chinese cabbage (brassica campestris ssp. pekinensis) were incubated in modified nn medium containing 10% sucrose in darkness at 33°c for one day followed by culture at 25°c. after 14 days of culture, microspores developed into embryos ranging from globular to cotyledonary stage. plants were regenerated after transfer of embryos to medium containing 3% sucrose and no plant growth regulators. | 1989 | 24233535 |
| infectious tymv rna from cloned cdna: effects in vitro and in vivo of point substitutions in the initiation codons of two extensively overlapping orfs. | full-length cdna of the 6.3 kb turnip yellow mosaic virus (tymv) genome was placed between a t7 promoter and a unique hind iii site. in vitro transcription of hind iii-linearized dna of clone ptymc yielded full-length rna transcripts. in inoculations of chinese cabbage protoplasts and plants, capped transcripts and virion rna had similar specific infectivities and produced similar systemic symptoms. we have used the ptymc clone in studies of the expression of two overlapping open reading frames ... | 1989 | 2473440 |
| first isolation of xanthomonas campestris from the blood of a chinese woman. | xanthomonas campestris isolated from the blood of a patient with a fever was first reported. xanthomonas campestris is a bacterium that can cause black rot of some vegetables, such as rape. chinese cabbage, etc. human infection due to x. campestris has not been reported so far. the characteristics of this organism, including morphology, staining, physiology and biochemistry were studied. we believe that x. campestris is also one of the opportunistic pathogens, which can infect compromised host. | 1990 | 2118062 |
| induction of nitroarenes in cigarette smoke condensate treated with nitrate. | samples of cigarette smoke condensates (cscs) treated with nitric acid or chinese cabbage pickles having a high nitrate content strongly mutated salmonella typhimurium strain ta98; the benzene/ethanol extract after these treatments induced 1800 and 820 revertants, respectively, per mg of extract for strain ta98 in the absence of s9 mix. the major mutagens in these materials were found to be 1-nitropyrene and 1,3-dinitropyrene on the basis of the result of gas chromatography/mass spectrometry and ... | 1990 | 2155483 |
| [antimutagenic activity of vegetable and fruit extracts against in-vitro benzo(a)pyrene]. | about 80% of the juices from twenty vegetables and fruits showed antimutagenic activity when tested in the presence of the mutagen and carcinogen benzo[a]pyrene using the ames' salmonella/microsome assay. in a standardized test system juices from raw celeriac, broccoli, red cabbage, carrots, green, peppers, lettuce, asparagus, apricots, red-currants, gooseberries, raspberries, and pineapple showed more than 50% inhibition. leek, kohlrabi, cucumber, zucchini, french beans, fennel leaves, rhubarb, ... | 1990 | 2336853 |
| [nucleic acid components in carbohydrate-rich food]. | the content of nucleic acid components in numerous foods, especially carbohydrate-rich ones, has been investigated. the data obtained for bases (purines and pyrimidines) were calculated as nucleic-acid equivalents (rna or dna); the imp content was calculated from the measured content of hypoxanthine. not only did cultivated plants such as cereals and pulses show a high rna-equivalent content but also vegetables such as spinach, leek, broccoli, chinese cabbage and cauliflower. we found the same r ... | 1990 | 1689090 |
| brassica taxonomy based on nuclear restriction fragment length polymorphisms (rflps) : 3. genome relationships in brassica and related genera and the origin of b. oleracea and b. rapa (syn. campestns). | rflps were used to study genome evolution and phylogeny in brassica and related genera. thirtyeight accessions, including 10 accessions of b. rapa (syn. campestris), 9 cultivated types of b. oleracea, 13 nine-chromosome wild brassicas related to b. oleracea, and 6 other species in brassica and allied genera, were examined with more then 30 random genomic dna probes, which identified rflps mapping to nine different linkage groups of the b. rapa genome. based on the rflp data, phylogenetic trees w ... | 1990 | 24226454 |
| turnip yellow mosaic virus rnas with anticodon loop substitutions that result in decreased valylation fail to replicate efficiently. | single and multiple nucleotide substitutions have been introduced into the anticodon loop of the trna-like structure of turnip yellow mosaic virus (tymv) genomic rna. we studied the effects of these mutations on in vitro valylation and on replication in chinese cabbage protoplasts and plants. only those mutants capable of efficient and complete valylation showed efficient replication in protoplasts and gave rise to systemic symptoms in whole plants. mutants that accepted valine inefficiently (in ... | 1991 | 2033666 |
| role of ethylene on de novo shoot regeneration from cotyledonary explants of brassica campestris ssp. pekinensis (lour) olsson in vitro. | the promotive effect of agno(3) and aminoethoxyvinylglycine (avg) on in vitro shoot regeneration from cotyledons of brassica campestris ssp. pekinensis in relation to endogenous 1-amino-cyclopropane-1-carboxylic acid (acc) synthase, acc, and ethylene production was investigated. agno(3) enhanced acc synthase activity and acc accumulation, which reached a maximum after 3 to 7 days of culture. acc accumulation was concomitant with increased emanation of ethylene which peaked after 14 days. in cont ... | 1991 | 16668148 |
| allelopathic dominance ofmiscanthus transmorrisonensis in an alpine grassland community in taiwan. | a study site located at 2600 m elevation in tartarchia anpu, nantou county, taiwan, exhibits a unique grassland community composed of two principal species,miscanthus transmorrisonensis andyushinia niitakayamensis, and 35 other species. the relative frequencies of the two species are 12% and 11%, while their relative coverages are 25% and 19.5%, respectively. the values for the remaining 35 species are lower than4% each, while species diversity of the community is -3.04839, indicating great dive ... | 1991 | 24258605 |
| a linkage map ofbrassica rapa (syn.campestris) based on restriction fragment length polymorphism loci. | a detailed linkage map ofb. rapa (syn.campestris) was constructed based on segregation of 280 restriction fragment length polymorphism loci, detected by using 188 genomic dna clones as probes on dnas from a f2 population of chinese cabbage 'michihilf'×'spring broccoli.' these genetic markers covered 1,850 centimorgans (cm) and defined ten linkage groups, which equals the haploid chromosome number of this species. extensive sequence duplication was evident by the detection of two or more segregat ... | 1991 | 24213173 |
| genetic diversity at isozyme and rflp loci in brassica campestris as related to crop type and geographical origin. | twenty accessions of brassica campestris, representing the major crop types and their geographical origin, were tested for gene frequency at five isozyme and four rflp loci. the majority of alleles (67%) were found in all geographic regions. nearly 3 times more alleles were detected at rflp loci than at isozyme loci. genetic diversity among crop types (with the exception of turnip) was similar to diversity estimates of geographical regions, implying that crops used for similar purposes (i.e., oi ... | 1992 | 24202754 |
| expression of orf-69 of turnip yellow mosaic virus is necessary for viral spread in plants. | turnip yellow mosaic virus (tymv) rna has two extensively overlapping open reading frames (orfs) encoding noncapsid proteins. the longest of these, orf-206, is essential for rna replication. we have investigated the expression and role of orf-69 (encoding a protein with a mw of 69k) using specific antiserum and mutant genomic rnas designed to interrupt orf-69 expression. tyc69 antiserum immunoprecipitated a protein with an apparent mw of 75k (p69) from in vitro translations of tymv rna. mutant r ... | 1992 | 1736523 |
| localization of cauliflower mosaic virus in the cell nucleus of brassica pekinensis l. | cauliflower mosaic virus (camv) particles were observed in the nuclei of xylem parenchyma cells in brassica pekinensis l. doubly infected by camv and turnip mosaic virus (tumv). camv particles were aggregated in the nucleoplasm but not embedded in viroplasms. this phenomenon was not detected in cell nuclei of mesophyll tissue. typical features associated with infection by either camv or tumv normally occurred in the cytoplasm of cells of both tissues: two types of viroplasms with embedded camv p ... | 1992 | 1410828 |
| turnip yellow mosaic virus variants produced from dna clones encoding their genomes. | full-length dsdna clones that encode the genomes of two australian turnip yellow mosaic isolates, tymv-bl and tymv-cl have been constructed. these clones were transcribed to give 6.3 kb capped ssrna which infects chinese cabbages to give symptoms indistinguishable from those produced by the parental viruses. extensions of up to 26 nucleotides at the 3' end of the tymv-bl clone delay infections, but virus particles isolated from these plants 4 weeks after inoculation contain rna with the original ... | 1992 | 1456892 |
| second-site suppressor mutations assist in studying the function of the 3' noncoding region of turnip yellow mosaic virus rna. | the 3' noncoding region of turnip yellow mosaic virus rna includes an 82-nucleotide-long trna-like structure domain and a short upstream region that includes a potential pseudoknot overlapping the coat protein termination codon. genomic rnas with point mutations in the 3' noncoding region that result in poor replication in protoplasts and no systemic symptoms in planta were inoculated onto chinese cabbage plants in an effort to obtain second-site suppressor mutations. putative second-site suppre ... | 1992 | 1501271 |
| increased viral yield and symptom severity result from a single amino acid substitution in the turnip yellow mosaic virus movement protein. | turnip yellow mosaic virus is a positive-strand rna virus that produces light green or yellow-green mosaic symptoms in chinese cabbage plants. we have characterized a strain that produces nearly uniform yellow-green chlorosis in systemically infected chinese cabbage leaves. the increased symptom severity is due to the single nucleotide substitution u1888-->c, which results in a tyrosine to histidine substitution in the movement protein encoded by orf-69. coding by the overlapping orf-206 is not ... | 1993 | 8324245 |