Publications

nucleotide sequence of a new bipartite geminivirus isolated from the common weed sida rhombifolia in costa rica.	the nucleotide sequence of infectious clones of a geminivirus from costa rica that infects sida rhombifolia was determined. sida golden mosaic virus (sigmv-co) has a bipartite genome (dnas a and b). computer analysis showed that the bipartite genome of sigmv-co resembles that of other whitefly-transmitted geminiviruses. the dna a (2605 nt) and dna b (2587 nt) components have little sequence homology other than within the common region (cr). analysis of dnas a and b showed that sigmv-co is closel ...	1997	9225056
coat protein gene replacement results in whitefly transmission of an insect nontransmissible geminivirus isolate.	geminiviruses are transmitted by whiteflies, leafhoppers, or treehoppers. the whitefly species bemisia tabaci (gennadius) is the most efficient vector of subgroup iii geminiviruses. an isolate of abutilon mosaic virus (abmv), a bipartite geminivirus, was not detectable in the dna extract from insects by southern blot analysis, nor was the isolate transmissible by the b-biotype of b. tabaci, although the virus dna was amplified (by pcr) from some insects. in contrast, sida golden mosaic virus (si ...	1997	9325236
virus-specific adaptations for the production of a pseudorecombinant virus formed by two distinct bipartite geminiviruses from central america.	most whitefly-transmitted geminiviruses possess bipartite genomes comprising dnas a and b. the production of viable pseudorecombinants by reassortment of infectious cloned components is generally limited to isolates/strains of a particular virus. following exchange of cloned genomic components of sida golden mosaic virus from costa rica (sigmv/co) and sida golden mosaic virus from honduras (sigmv/ho(yv)), the pseudorecombinant viruses were infectious in various plant species. three dna b compone ...	2000	10936098
host range and symptom variation of pseudorecombinant virus produced by two distinct bipartite geminiviruses.	within the whitefly group only the species bemisia tabaci (gennadius) is the vector. most whitefly-transmitted geminiviruses possess bipartite dna genomes, dnas a and b. although they are closely related to each other, the production of viable pseudorecombinants between bipartite geminiviruses by reassortment of infectious cloned components is generally limited to strains of a particular virus. following exchange of cloned genomic components of sida golden mosaic virus (sigmv/hoyv) and abutilon ...	2000	10963348
exchange of three amino acids in the coat protein results in efficient whitefly transmission of a nontransmissible abutilon mosaic virus isolate.	geminiviruses are transmitted in a circulative manner by whiteflies, leafhoppers, or treehoppers. the whitefly species bemisia tabaci (genn.) is the vector for members of the genus begomovirus. the closely related bipartite central american begomoviruses abutilon mosaic virus (abmv), sida golden mosaic virus originating from costa rica (sigmv-cr), and sida golden mosaic virus originating from honduras (sigmv-hoyv) were used to study transmission by their insect vector. the abmv isolate is defect ...	2001	11883001
tomato mottle taino virus pseudorecombines with pymv but not with tomov: implications for the delimitation of cis- and trans-acting replication specificity determinants.	over the last decade, the tomato production in cuba has been affected by new whitefly-associated diseases. in addition to the well-documented presence of tomato yellow leaf curl virus (tylcv) along the island, the occurrence of bipartite begomoviruses has also been reported. one of them, tentatively named tomato mottle taino virus (tomotv), has now been cloned and characterized at the molecular level. its genomic organization is similar to other bipartite geminiviruses. phylogenetic analyses pla ...	2003	14505083
preliminary identification and coat protein gene phylogenetic relationships of begomoviruses associated with native flora and cultivated plants from the yucatan peninsula of mexico.	a number of native and cultivated eudicots in the yucatan peninsula of mexico (ypm) exhibit symptoms associated with virus infection. symptomatic leaves were collected and assessed for begomoviral detection using polymerase chain reaction (pcr), and universal primers that amplify a fragment of the coat protein gene (core cp). begomovirus were detected in nine native and seven cultivated species, representing seven eudicot families. dna extracts from the 16 hosts were used for pcr amplification a ...	2007	17682933
molecular characterization and phylogeny of two begomoviruses infecting malvastrum americanum in jamaica: evidence of the contribution of inter-species recombination to the evolution of malvaceous weed-associated begomoviruses from the northern caribbean.	two distinct full-length begomovirus dna-a components and a dna-b component were pcr amplified, cloned and sequenced from jamaican malvastrum americanum plants exhibiting yellow mosaic symptoms. whereas one of the dna-a components is from a potentially new species that we have tentatively named malvastrum yellow mosaic helshire virus (maymhv), the other dna-a and the dna-b form a cognate pair and represent a new virus species tentatively named malvastrum yellow mosaic jamaica virus (maymjv). the ...	2010	20024609
two novel begomoviruses belonging to different lineages infecting rhynchosia minima.	rhynchosia minima is a host for several begomoviruses, both in the old world and the new world. in cuba, a whitefly-transmitted disease causing yellow mosaic symptoms, suggested to be of viral origin, was described more than 30 years ago in r. minima, but no information about the nature of the viruses infecting this weed in this country is available to date. here, we report the detection of isolates of two novel begomovirus species infecting r. minima in cuba, which we proposed be named rhynchos ...	2010	20852903
Begomoviruses infecting weeds in Cuba: increased host range and a novel virus infecting Sida rhombifolia.	As a result of surveys conducted during the last few years to search for wild reservoirs of begomoviruses in Cuba, we detected a novel bipartite begomovirus, sida yellow mottle virus (SiYMoV), infecting Sida rhombifolia plants. The complete genome sequence was obtained, showing that DNA-A was 2622 nucleotides (nt) in length and that it was most closely related (87.6% nucleotide identity) to DNA-A of an isolate of sida golden mosaic virus (SiGMV) that infects snap beans (Phaseolus vulgaris) in Fl ...	2011	21964921
the molecular characterisation of a sida-infecting begomovirus from jamaica.	the complete dna sequence of both genome components of a new begomovirus (sida golden mosaic buckup virus-[jamaica:st. elizabeth:2004]; sigmbuv-[jm:se:04]) was determined from a field-infected sida sp. sample from buckup, st. elizabeth, jamaica. phylogenetically, both genome components of sigmbuv-[jm:se:04] are most closely related to malvaceous weed-infecting floridian and mexican begomoviruses. its dna-b is a recombinant molecule, the majority of which was derived from a virus resembling sida ...	2014	23933798