Publications

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on the rate of oxygen consumption by fertilized and unfertilized eggs : i. fucus vesiculosus.1. the unfertilized eggs of fucus vesiculosus, in the dark, consume about 5.2 mm.(3) o(2) per hour per 10 mm.(3) eggs. 2. with an illumination of 100,000 foot candles in photosynthesis they liberate more than twice as much oxygen as they consume. 3. the actively swimming antherozoids or sperm of fucus consume oxygen at a very high rate: 25.5 mm.(3) o(2) per hour per 10 mm.(3) antherozoids. 4. immediately following fertilization, in the dark, the fucus eggs increase the rate of oxygen consumption ...193119872637
on the rate of oxygen consumption by fertilized and unfertilized eggs : v. comparisons and interpretation.1. the rate of oxygen consumption by eggs may not merely undergo no change at fertilization, as in the case of the starfish, but it decreases to about half in chaetopterus and in cumingia. 2. the absolute rate of oxygen consumption in mm.(3) o(2) per hour per 10 mm.(3) eggs differs widely in several species of unfertilized eggs. it is very low in the sea urchin, intermediary in nereis, and high in chaetopterus and cumingia. the range for these eggs is approximately 0.4 to 3.1 mm.(3) o(2) per hou ...193319872719
the algae: the lipochromes of fucus vesiculosus. 193516745801
on the nitrogenous constituents of fucus vesiculosus. 195313129263
biosynthesis of radioactive mannitol from c1402 by fucus vesiculosus. 195813592252
potentiation of photosynthetic oxygen evolution in red light by small quantities of monochromatic blue light.growth of the giant unicellular green alga, acetabularia crenulata, stops in red light of broad spectral composition, but can be restored by the addition of small quantities of blue light. long-term records of o(2) evolution indicate that the photosynthesis of acetabularia responds in a parallel manner to blue light. cells photosynthesizing at a light-limited rate in white light were given red light at an intensity that served to match or somewhat increase the instantaneous rate of o(2) producti ...196616656414
thermal stability of threonine in the presence of a marine polyphenolic material.the rate of decomposition of threonine in 0.01m aqueous solution is unaffected by an equimolar concentration of pyrocatechol, but is increased by the addition of an extracellular polyphenolic material (3 mg/ml) produced by fucus vesiculosus. glycine, a pyrolytic product of threonine, behaves similarly.196617746339
the influence of epiphytic bacteriae on auxin metabolism.plants are settled by epiphytic bacteriae able to convert tryptophan to iaa. this bacterial activity is abolished by chloramphenicol and streptomycin but not by penicillin. tryptophan conversion to iaa by plant parts or enzyme preparations is far more intensive in non-sterile conditions than in sterile ones. this is true for all investigated objects: helianthus annuus, phaseolus vulgaris, pisum sativum, triticum vulgare, zea mays, enteromorpha compressa, fucus vesiculosus, furcellaria fastigiata ...196624557783
[fatty acids on the galactolipids from the red alga batrachospermum moniliforme and the brown alga fucus vesiculosus]. 19685706136
amino acid incorporation in developing fucus embryos.the incorporation of (14)c-leucine and (14)c-amino acid mixture into protein in unfertilized eggs and developing embryos of the brown alga fucus vesiculosus l. was studied. bacterial contamination was initially a problem, but it was found that the addition of 40 mug/ml chloramphenicol to the incubation medium would inhibit bacterial protein synthesis without affecting early development of the fucus embryos. the kinetics of uptake and incorporation of (14)c-leucine into the trichloroacetic acid-s ...196816656865
changes in the polysomal pattern after fertilization in fucus eggs.at high potassium concentrations it is possible to isolate largely intact polysomes from fertilized and unfertilized fucus vesiculosus eggs, and to study the distribution pattern of ribosomal particles in a sucrose gradient. the changes in the ribosomal pattern before and after fertilization reflect the time course of protein synthesis. in the unfertilized egg there is a pool of inactive light polysomes which are fairly resistant to ribonuclease treatment, whilst a heavier polysomal fraction sho ...196924515590
[determination of fucosterol in fucus vesiculosus l. and in extracts of fucus vesiculosus l]. 19705502285
sulphated polysaccharides in brown algae. 3. the native state of dfucoidan in ascophyllum nodosum and fucus vesiculosus. 19705501728
[polar lipids from pelvetia canaliculata (l) dech. and thur., fucus vesiculosus l. and fucus servatus l]. 19744374970
cell wall assembly in fucus zygotes: i. characterization of the polysaccharide components.fertilization triggers the assembly of a cell wall around the egg cell of three brown algae, fucus vesiculosus, f. distichus, and f. inflatus. new polysaccharide polymers are continually being added to the cell wall during the first 24 hours of synchronous embryo development. this wall assembly involves the extracellular deposition of fibrillar material by cytoplasmic vesicles fusing with the plasma membrane. one hour after fertilization a fragmented wall can be isolated free of cytoplasm and co ...197616659652
fine-structural studies of the gametes and embryo of fucus vesiculosus l. (phaeophyta). ii. the cytoplasm of the egg and young zygote.following fertilization, there are rapid changes in the appearance of the fucus egg. large electron-translucent vesicles (v1) accumulate fibrillar material, and following pronuclear fusion, they are largely electron-opaque. these vesicles (v1) are formed originally in unfertilized eggs by smooth endoplasmic reticulum (ser) after release of the eggs from the oogonium. golgi complex hypertrophy follows fertilization, and this increased activity continues throughout early embryogenesis. wall format ...1976944190
fine-structural studies of the gametes and embryo of fucus vesiculosus l. (phaeophyta). i. fertilization and pronuclear fusion.in the marine brown alga, fucus vesiculosus l., the sperm pronucleus is delimited by an envelope following penetration of the eff by the sperm. this envelope disintegrates as the pronucleus begins its migration through the cytoplasm of the egg. the highly condensed chromatin of the sperm pronucleus disperses slightly following disintegration of the envelope. microtubules of unknown origin are associated with the sperm pronucleus during its migration. the flagellar microtubules remain in the peri ...1976944189
physodes and the phenolic compounds of brown algae. isolation and characterization of phloroglucinol polymers from fucus vesiculosus (l.)a series of vanillin-reactive compounds has been isolated from extracts of the brown alga fucus vesiculosus. the first three members of this series have been examined by mass spectrometry, nuclear magnetic resonance spectrometry, and chemical methods, and were shown to be phloroglucinol, its phenyl-linked dimer, and a timer also composed of phloroglucinol units. evidence is presented for the presence of tetrameric, pentameric, and hexameric phloroglucinol derivatives. polymeric vanillin-reactive ...19761252984
[structure, amount and composition of sulfuric, sulfonic and phosphoric esters of glycosyldiglycerides of three fucacea].the mono and polyester glycosyl sulfates or phosphate diglycerides account for a group of polar lipids which is found in large amounts in the three fucacae that are studied: pelvetia canaliculata (l) deen and thur, fucus vesiculosus (l), fucus serratus (l). these polar lipids have been shown to have complex structures, most of them are unknown in the present nomenclature. both quantity and composition of these polar lipids are species characteristics, forming the equipment of polar lipids for ev ...19761016655
fine-structural studies of the gametes and embryo of fucus vesiculosus l. (phaeophyta). iii. cytokinesis and the multicellular embryo.condensation of the chromosomes during the first cell division following fertilization of the brown alga fucus vesiculosus l. is accompanied by the almost complete disappearance of the nuclear envelope. golgi vesicles and other small vesicles appear within the spindle, which has paired centrioles at each end. a large amount of rough endoplasmic reticulum is in the surrounding cytoplasm during mitosis, and many vesicles at the spindle margin are encircled by stacks of endoplasmic reticulum. annul ...1977893547
asymmetrical jelly secretion of zygotes of pelvetia and fucus: an early polarization event.five hours before germination the zygotes of pelvetia fastigiata adhere to their substrate. a jelly layer covers the entire cell but most of the transparent jelly, artificially outlined by a layer of resin beads, is secreted at the prospective rhizoid pole. if the direction of the growth-orienting light is shifted after the asymmetrical secretion has already started, the direction of the secretion is also shifted. the polarization axis can be predicted by the site of the intensive jelly secretio ...197824414363
[agglutinins in marine brown algae. dedicated to professor dr. h. knöll on his 65th birthday].extracts from the brown algae fucus vesiculosus, f. serratus, and halidrys siliquosa agglutinated human erythrocytes of groups a, b, o, and ab as well as erythrocytes from some animal species, ascites tumour cells from mice, and streptococci of many groups of the lancefield system. the extract of chorda filum did not show haemagglutination but agglutinated ascites tumour cells and streptococci of a few groups.1978567898
longitudinal profiles of carbon dioxide fixation capacities in marine macroalgae.fucus serratus l., fucus spiralis l., and fucus vesiculosus l. (fucales, phaeophyceae) as well as laminaria digitata (huds.) lamour., laminaria hyperborea (gunn.) fosl., and laminaria saccharina (l.) lamour. (laminariales, phaeophyceae) have been investigated for the distribution of enzymic co(2) fixation capacities via phosphoenolpyruvate carboxykinase (ec 4.1.1.32) (pep-ck) and via ribulose-1,5-bisphosphate carboxylase (ec 4.1.1.39) (rubp-c) in different regions of the thalli. the maximum of p ...197816660467
tracing food webs with stable hydrogen isotopes.the hydrogen isotopic content of an animal's food, not water, determines that animal's hydrogen isotopic content. liver and muscle tissue from mice reared on a diet such that the ratio of deuterium to hydrogen (dih) of their food and water was kept constant, have the same average d/h ratio as the food source. in a simple, natural population of snails and their possible algal diets, littorina obtusata (northern atlantic intertidal snails that feed almost exclusively on the brown alga fucus vesicu ...198017745967
polyphenols in brown algaefucus vesiculosus andascophyllum nodosum: chemical defenses against the marine herbivorous snail,littorina littorea.polyphenols from two brown algae,fucus vesiculosus (l.) andascophyllum nodosum (l.) le jolis, inhibited feeding by the herbivorous snail,littorina littorea. the active compounds were characterized as phloroglucinol polymers with a wide molecular weight range (mol wt <30,000 to >300,000) by spectroscopic, ultrafiltration, thin-layer chromatographic, and chemical degradation data. as little as 1% (dry wt) polyphenol in food reduced feeding by more than 50%, and polyphenolic extracts inhibited feed ...198124420835
selective interaction of a fucus vesiculosus lectin-like mucopolysaccharide with several candida species.a complex carbohydrate specific lectin-like mucopolysaccharide extracted from the brown alga fucus vesiculosus was found to agglutinate candida guilliermondii cells but not those of other species of the same genus (except for a weak agglutination with c. krusei). the selective binding of this mucopolysaccharide correspondingly affected the growth of the yeasts. the results suggest that there are notable differences--at least with respect to the receptors for the f. vesiculosus mucopolysaccharide ...19836223551
purification and partial characterization of a fucus vesiculosus agglutinin.fucus vesiculosus agglutinin has been purified to homogeneity by conventional chromatographic procedures and characterized as a mucopolysaccharide with 90% carbohydrate content. estimated molecular weight is about 2 x 10(6) daltons. it has no sub-unit structure and its isoelectric point is 3.2. it contains 1.23% s, 0.24% ca and 0.06% p. agglutinin mediated sheep red blood cell agglutination was inhibited only by glycoproteins with complex lateral oligosaccharide chains resembling some of the oli ...19836867442
toxicity of an algal mucopolysaccharide for escherichia coli and neisseria meningitidis strains.several bacterial strains of clinical significance have been tested to assess the toxic effect of a lectin-like algal mucopolysaccharide from fucus vesiculosus on their growth. the toxic effect of the mucopolysaccharide has been found to be exerted only on escherichia coli and neisseria meningitidis strains. the degree of toxicity, measured by the effect on the growth of the bacteria, is variable depending on the strains of e. coli tested, whereas with n. meningitidis the results obtained indica ...19846237397
observations of exocytosis in fucus vesiculosus gametes using video-enhanced light microscopy: a video report. 19846722865
[accumulation of polycyclic arenes in baltic sea algae].the paper presents data on the level of benzo(a)pyrene (bp) and some other polycyclic arenes in alga and phanerogam specimens from different gulfs of the baltic sea. algae were shown to absorb bp from sea water. the mean concentration of bp in sea water was under 0.004 microgram/1, while in algae it ranged 0.1-21.2 micrograms/kg dry weight. algae accumulate bp to a higher degree than phanerogams. the highest concentrations of bp were found in algae enteromorpha while the lowest ones in furcellar ...19854060672
distribution of lead and cadmium in trophic levels of some marine organisms.the concentrations of lead and cadmium in two species of algae, two species of invertebrates, and one species of fish (from fehmern baltic sea) were determined using atomic absorption spectrophotometry. the concentrations of these metals at the producer level (algae) were always higher than at the consumer level. also, among the consumers there was an obvious difference according to feeding habits, in such a way that they could be ranked according to their high content of lead and cadmium as fol ...19873448857
concentrations of cadmium and other metals in fucus vesiculosus l. and fontinalis dalecarlica br. eur. from the northern baltic sea and the southern bothnian sea.concentrations of cd and ten other metals (al, co, cr, cu, fe, mn, ni, pb, v and zn) were determined in the brown seaweed fucus vesiculosus l. and the aquatic moss, fontinalis dalecarlica br. eur. from the northern baltic sea and the southern bothnian sea. elevated concentrations of metals were found in samples taken close to densely populated areas, such as stockholm and nynäshamn. very high concentrations of especially zn were found in both fucus and fontinalis samples taken from the area sout ...198815092626
studies on metal content in the brown seaweed, fucus vesiculosus, from the archipelago of stockholm.concentrations of eleven metals (al, cd, co, cr, cu, fe, mn, ni, pb, v and zn) were determined in the brown seaweed fucus vesiculosus collected from the archipelago of stockholm. several factors which influence the metal content in the seaweed have been studied, including errors caused by epiphytes, sea exposure and differences depending on which part of the seaweed is analysed. it is concluded that, if all these factors are considered, fucus vesiculosus plants are excellent bio-indicators of me ...198815092658
hypoglycemic activity of several seaweed extracts.the hypoglycemic activity of several seaweed extracts on rabbits was studied. ethanol extracts of laminaria ochroleuca, saccorhiza polyschides and fucus vesiculosus were administered orally to normal animals and their effects on glycemia and triglyceridemia evaluated. crude polysaccharides and protein solutions from himanthalia elongata and codium tomentosum were also assayed. polysaccharides and proteins from h. elongata caused a significant reduction in blood glucose 8 h after intravenous admi ...19892615424
calmodulin-binding proteins are developmentally regulated in gametes and embryos of fucoid algae.calcium-binding proteins and calmodulin-binding proteins were identified in gametes and zygotes of the marine brown algae fucus vesiculosus, fucus distichus, and pelvetia fastigiata using gel (sds-page) overlay techniques. a calcium current appears to be important during cell polarization in fucoid zygotes (k.r. robinson and l.f. jaffe, 1975, science 187, 70-72; k.r. robinson and r. cone, 1980, science 207, 77-78), but there are no biochemical data on calcium-binding proteins in these algae. by ...19892912798
physiological effects in fish exposed to effluents from mills with six different bleaching processes.immature rainbow trout (salmo gairdneri r.) were continuously exposed in a flow-through system to a total of six different bleached kraft mill effluents (bkme) at two exposure concentrations (400 and 2000 times dilution) for 7 weeks, during three consecutive years (1982-1984). the fish were exposed in outdoor tanks connected to the outgoing water from a parallel mesocosms study simulating the baltic littoral (fucus vesiculosus) zone. after exposure, hematological, osmoregulational) and mixed fun ...19902311561
the fast block against polyspermy in fucoid algae is an electrical block.fertilization potentials in pelvetia fastigiata, fucus vesiculosus, and fucus ceranoides were studied to examine whether eggs of fucoid algae have an electrical block against polyspermy. the resting potential of eggs of all species was about -60 mv, depolarizing, respectively, to -24 +/- 5 mv (sd, n = 9) for 7.5 +/- 2.1 (n = 8) min, -26 +/- 5 (n = 9) mv for 6.4 +/- 2.3 (n = 9) min, and -24 +/- 6 (n = 5) mv for 6.7 +/- 1.9 (n = 4) min. the depolarization was slower, and the fertilization potentia ...19911995405
seasonal variation of radionuclides in fucus vesiculosus l. from the oresund, southern sweden.the activity concentrations of radionuclides (60co, 54mn, 137cs, 40k) in whole plants of fucus vesiculosus of different ages, and also in tissues of different ages from the same plants were investigated in the vicinity of the barsebäck nuclear power plant in southern sweden. activity concentration differed between the tissues of different age of the plants, depending on the radionuclide as well as the time of year. the highest concentrations of 60co and 54mn were measured in the older parts of t ...199115092091
biosorption of cadmium by biomass of marine algae.biomass of nonliving, dried brown marine algae sargassum natans, fucus vesiculosus, and ascophyllum nodosum demonstrated high equilibrium uptake of cadmium from aqueous solutions. the metal uptake of cadmium from aqueous solutions. the metal uptake by these materials was quantitatively evaluated using sorption isotherms. biomass of a. nodosum accumulated the highest amount of cadmium exceeding 100 mg cd(2+)/g (at the residual concentration of 100 mg cd/l and ph 3.5), outperforming a commercial i ...199318609626
a new procedure for the isolation of anti-hiv compounds (polysaccharides and polyphenols) from the marine alga fucus vesiculosus.anti-hiv-active polysaccharides and polyphenols were isolated from the brown seaweed fucus vesiculosus by hot h2o extraction of both the intact and the homogenized algae. this was followed by xad2 chromatography and by sequential precipitation of the non-adsorbed compounds with glacial hoac and thereafter with etoh. the precipitate was solubilized, dialyzed against distilled h2o, and chromatographed on sp-sephadex c25 and on qae-sephadex a25. this was followed by gel filtration on sephadex g50 a ...19937684438
a revised structure for fucoidan may explain some of its biological activities.fucoidan from fucus vesiculosus inhibits human sperm-zona pellucida binding and blocks the zona pellucida-induced acrosome reaction in human sperm. fucoidan also potently inhibits selectin-mediated adhesion of leukocytes to vascular endothelium. to understand the molecular basis for fucoidan's inhibition of specific cell adhesion events, we have investigated the structure of this fucan using definitive methods of carbohydrate structural analysis. we report the revised average structure for fucoi ...19938408031
effects of pulp mill chlorate on baltic sea algae.the long-term effects of pulp mill chlorate on different algal species of the baltic sea were studied in land-based model ecosystems simulating the littoral zone. brown algae (phaeophyta) exhibited an extraordinarily high sensitivity to chlorate and pulp mill effluents containing chlorate. all brown algal species ceased growth or showed major signs of toxicity at all concentrations tested, down to microgram per litre levels. ec50 levels for growth of fucus vesiculosus were about 80-100 microg cl ...199415091680
biosorption of lead and nickel by biomass of marine algae.screening tests of different marine algae biomas types revealed a high passive biosorptive uptake of lead up to 270 mg pb/g of biomass in some brown marine algae. members of the order fucales perfomed particularly well in this descending sequence: fucus > ascophyllum > sargassum. although decreasing the swelling of wetted biomass particles, their reinforcement by crosslinking may significantly affect the biosorption performance. lead uptakes up to 370 mg pb/g were observed in crosslinked fucus v ...199418615510
isolation and partial characterization of a novel amino sugar-containing fucan sulfate from commercial fucus vesiculosus fucoidan.commercial crude fucoidan (sigma) from the brown seaweed fucus vesiculosus was fractionated into its polysaccharide components by gel filtration and anion-exchange chromatography to clarify the structure-anticoagulant activity relationship. the products comprised a wide spectrum of fucans ranging from typical fucoidans (major components) containing mainly fucose, sulfate, and no uronic acid to low sulfate-containing heteropolysaccharide-like fucans (minor components) being composed of neutral su ...19948181008
use of seaweeds for monitoring trace elements in coastal waters.concentrations of a wide range of trace elements: arsenic, cadmium, cobalt, chromium, hafnium, nickel, thorium, uranium, zinc and the rare earth elements, cerium, europium, samarium, terbium and ytterbium were determined by instrumental neutron activation analysis in the brown alga,fucus vesiculosus from eckwarder hörne, north sea and from rügen, baltic sea. another brown alga,sargassum filipendula from sri lanka, indian ocean (representing an unpolluted control station) was similarly investigat ...199624194376
successful external fertilization in turbulent environments.mathematical and experimental simulations predict that external fertilization is unsuccessful in habitats characterized by high water motion. a key assumption of such predictions is that gametes are released in hydrodynamic regimes that quickly dilute gametes. we used fucoid seaweeds to examine whether marine organisms in intertidal and subtidal habitats might achieve high levels of fertilization by restricting their release of gametes to calm intervals. fucus vesiculosus l. (baltic sea) release ...199611607682
statistical evaluation of ecosystem properties influencing the uptake of as, cd, co, cu, hg, mn, ni, pb and zn in seaweed (eucus vesiculosus) and common mussel (mytilus edulis).in order to evaluate the influence of geographically varying marine ecosystem properties on the uptake of trace elements in bioindicators, samples were taken of seaweed (fucus vesiculosus) and common mussel (mytilus edulis) along the north sea and baltic sea coast. seasonal variations of the bioindicator status were minimized by sampling within 1 month. ecosystem properties considered were the geographical position, the salinity and the concentrations of the macroelements ca, fe, k, mg, na, p an ...19979397597
biochemical responses of the marine macroalgae ulva lactuca and fucus vesiculosus to cadmium and copper-from sequestration to oxidative stress. 19979056961
anticoagulant fucoidan fractions from fucus vesiculosus induce platelet activation in vitro.anticoagulant fucoidan fractions of different molecular weight and sulfate content were prepared and investigated for their effects on platelet function in vitro. the fucoidan fractions were incubated with human platelet rich plasma (prp) at concentrations of 5, 10 and 50 micrograms/ml. platelet activation was subsequently studied by a standard aggregation assay and flow cytometric determination of the activation dependent platelet-surface markers cd62p (p-selectin, gmp-140) and cd63 (gp53). all ...19979101640
cross-species induction and enhancement of antimicrobial activity produced by epibiotic bacteria from marine algae and invertebrates, after exposure to terrestrial bacteria.antibiotic producing marine bacteria isolated from surfaces of the marine alga fucus vesiculosus and the nudibranch archidoris pseudoargus were exposed to live cells of staphylococcus aureus, pseudomonas aeruginosa, escherichia coli and heat-killed cells of staph. aureus. twelve out of the 16 marine strains tested showed enhanced antimicrobial activity towards staph. aureus, e. coli and ps. aeruginosa following this exposure. three out of seven strains tested showed enhanced antimicrobial activi ...19989750317
role of algae in fate of carcinogenic polycyclic aromatic hydrocarbons in the aquatic environment.polycyclic aromatic hydrocarbons (pahs) represent an ecotoxicologically relevant, combustion-related substance group. the bioconcentration and transformation of a priority pah, benzo[a]pyrene (bap), by brown (fucus vesiculosus and chorda filum), red (furcellaria lumbricalis), green (enteromorpha intestinalis, and cladophora glomerata), and chara (chara aspera) algae have been studied. a flux budget was made of the amounts of bap that are accumulated and metabolized by different algae during an e ...19989756694
fucus vesiculosus: a nephrotoxic alga? 19989509485
quality assurance in measuring the elemental composition of the alga fucus vesiculosus.algae are increasingly used for the purpose of environmental biomonitoring, for instance in the long-term program of the federal environmental specimen bank of germany. therefore, for the studies presented here, freeze-dried samples of fucus vesiculosus collected from the north sea shores were analyzed for a broad range of elements by instrumental neutron activation analysis (inaa), while inductively coupled plasma atomic emission spectrometry (icp-aes) and mass spectrometry (icp-ms) were carrie ...199911529088
identification and characterization of a recombinant metallothionein protein from a marine alga, fucus vesiculosus.a cdna library was constructed from macroalgae adapted to prolonged elevated environmental copper levels. to investigate the possible existence of a metallothionein (mt) gene, the library was screened with degenerate probes designed using plant mt cysteine-rich motifs. a gene was identified (1229 bp) with a putative open reading frame (204 bp) encoding a 67-amino-acid protein exhibiting several characteristic features of mt proteins, including 16 cysteine residues (24%) and only one aromatic res ...199910024535
evaluation of the contamination of marine algae (seaweed) from the st. lawrence river and likely to be consumed by humans.the goal of the study was to assess the contamination of marine algae (seaweeds) growing in the st. lawrence river estuary and gulf of st. lawrence and to evaluate the risks to human health from the consumption of these algae. algae were collected by hand at low tide. a total of 10 sites on the north and south shores of the st. lawrence as well as in baie des chaleurs were sampled. the most frequently collected species of algae were fucus vesiculosus, ascophyllum nodosum, laminaria longicruris, ...199910092431
continuing contamination of north atlantic and arctic waters by sellafield radionuclides.discharges of 99tc and 129i from the reprocessing plant at sellafield have increased significantly since the mid-1990s, against the overall trend of most other radionuclides. the 'pulsed' release of 99tc has provided an opportunity to study transport pathways and transit times in uk waters, the north sea and beyond. transit times estimated from the 99tc data are signifcantly shorter than rates reported previously using other radiotracers. the possible reasons for this are discussed. a comparison ...199910568270
evidence for bioadhesive effects of polysaccharides and polysaccharide-containing herbs in an ex vivo bioadhesion assay on buccal membranes.aqueous extracts of polysaccharide-containing plants are widely used in therapy for irritated mucus membranes in the pharynx region. in order to prove the existence of mucilaginous effects of polysaccharide hydrocolloids on epithelia an ex vivo system based on porcine buccal membranes was established. the tissue culture was stable and there was no indication of cytolytic processes during the 5 hour incubation period. this was confirmed through histological studies and the respective ldh values a ...200010705734
obesity and medicinal plants.the effects of some medicinal plants (i.e. fucus vesiculosus, citrus aurantium) that are claimed to be useful in the treatment of obesity are reviewed.200010930716
effects of natural product extracts on contraction and mechanical properties of fibroblast populated collagen gel.fibroblast-populated collagen gel cultures have been used as a dermal model of wound contraction and granulation in the wound healing process and as an in vitro model of dermal tissue. we evaluated the effects of various natural product extracts on collagen gel contraction-promoting activity and mechanical properties (relaxation time) using this model, and observed that some natural product extracts, such as hibamata extract (from fucus vesiculosus) promoted gel contraction and increased the rel ...200010726881
different roles of feeding and protection in diel microhabitat choice of sexes in idotea baltica.in idotea baltica, a marine isopod that lives and feeds on the brown alga fucus vesiculosus, microhabitat choice differs between sexes so that males are found more often than females on the light-coloured and exposed apical parts of the alga. we investigated how the requirements of avoiding visual predators and feeding were related to microhabitat choice in relation to diurnal and life-cycle stage in males and females. faced with a choice between an apical and a basal piece of the alga, females ...200028308335
fucoidan is the active component of fucus vesiculosus that promotes contraction of fibroblast-populated collagen gels.the fibroblast-populated collagen gel culture method has been evaluated as a dermal model of wound contraction and granulation in tissues during the wound healing process and as an in vitro model of dermal tissue. we previously reported that an extract of fucus vesiculosus promoted fibroblast-populated collagen gel contraction and that the promotion of the gel contraction was due to the increased expression of integrin alpha2beta1 on the surface of the fibroblasts. in this study, we investigated ...200011041247
photosynthesis during desiccation in an intertidal alga and a land plant.this study was undertaken to determine how photosynthesis tolerates desiccation in an intertidal alga fucus vesiculosus l. and a terrestrial sunflower helianthus annuus l. photosynthetic o2 evolution generally was inhibited at low water potentials (psiw) but more in sunflower leaves than in fucus fronds at the same psiw. as psiw decreased, less carbon accumulated in an organic carbon store in fucus. the inhibition of photosynthesis appeared to be mostly biochemical because it could not be preven ...200010805598
technetium-99 in the irish marine environment.technetium-99 activity concentrations in seawater and biota from irish coastal waters are presented. time series measurements of 99tc in seawater and fucus vesiculosus from the western irish sea show that activity concentrations have increased in line with the increase in discharges of 99tc from sellafield. the peak in activity concentrations in both seawater and fucus vesiculosus occurred in 1997 approximately two years after the peak in 99tc discharges. the highest activity concentration recor ...200111468819
a disaccharide repeat unit is the major structure in fucoidans from two species of brown algae.the predominant repeating structure of a fraction of the fucoidan from ascophyllum nodosum prepared by acid hydrolysis and centrifugal partition chromatography (lmwf) was established as: [-->3)-alpha-l-fuc(2so3-)-(1-->4)-alpha-l-fuc(2,3diso3-)-(1]n by nmr spectroscopy and methylation analysis. the proton and carbon nmr spectra of this unit have been assigned and found to correspond with features in the spectra of the whole purified fucan from a. nodosum which account for most of the integrated i ...200111269406
heavy metal contamination of brown seaweed and sediments from the uk coastline between the wear river and the tees river.the concentration of fe, mn, zn, cu, pb, ni, cr, cd, and ag were determined in the brown alga fucus vesiculosus and intertidal surface sediments from coastal locations of northeast england. levels of heavy metals similar to those of polluted areas of the british coastline were detected. there is evidence of contamination (especially with zn and pb) in sediments from sites affected by colliery spoil and from the wear estuary. the pelitic fraction ( < 63 microm) is usually more enriched in heavy m ...200111341296
treatment of human skin with an extract of fucus vesiculosus changes its thickness and mechanical properties.recently the researchers found that an extract of fucus vesiculosus, which is a type of seaweed, promotes the contraction of fibroblast-populated collagen gels through increased expression of integrin molecules. in this study, they investigated the effects of topical application of an aqueous extract of this alga on the thickness and the mechanical properties of human skin. a gel formulation that included 1% of the extract was applied topically to human cheek skin twice daily for five weeks. a s ...200211917251
evaluation of 20 years of environmental monitoring data around swedish nuclear installations.twenty years of environmental monitoring data around the swedish nuclear power plants and the studsvik research facilities have been evaluated. in the marine environment, fucus vesiculosus generally has high activity concentrations and the presence of a large variety of radionuclides in comparison with other bioindicators. however, for single nuclides the detection frequency was higher for 110mag in littorina spp and for 152eu in macoma baltica in comparison with other bioindicators. close to th ...200212363266
biomonitoring heavy metals in estuaries: a field comparison of two brown algae species inhabiting upper estuarine reaches.biomonitoring dissolved heavy metals within estuaries, particularly at their upper reaches, frequently has to rely on several biomonitors; rarely a single species thrives all along the salinity gradient. to properly do so, it must be established whether those biomonitors actually accumulate heavy metals alike. in this study, two brown seaweeds from the upper section of three nw spain estuaries--the widely-known fucus vesiculosus and the estuarine fucus ceranoides--were compared as metal biomonit ...200212002281
potential antioxidant capacity of sulfated polysaccharides from the edible marine brown seaweed fucus vesiculosus.fucus vesiculosus was sequentially extracted with water at 22 degrees c (fraction 1 (f1)) and 60 degrees c (f2), and with 0.1 m hcl (f3) and 2 m koh (f4) at 37 degrees c. soluble fractions (42.3% yield) were composed of neutral sugars (18.9-48 g/100 g), uronic acids (8.8-52.8 g/100 g), sulfate (2.4-11.5 g/100 g), small amounts of protein (< 1-6.1 g/100 g), and nondialyzable polyphenols (0.1-2.7 g/100 g). the main neutral sugars were fucose, glucose, galactose, and xylose. infrared (ir) spectra o ...200211829654
characterization of a new alpha-l-fucosidase isolated from the marine mollusk pecten maximus that catalyzes the hydrolysis of alpha-l-fucose from algal fucoidan (ascophyllum nodosum).algal fucoidan is an alpha-l-fucose-based polysaccharide endowed with important biological properties for which the structure has not yet been fully elucidated. in an attempt to implement new enzymatic tools for structural study of this polysaccharide, we have found a fucosidase activity in the digestive glands of the common marine mollusk pecten maximus, which is active on a fucoidan extracted from the brown algae ascophyllum nodosum. we now report the purification and characterization of this ...200212042250
biotransformation and accumulation of arsenic in soil amended with seaweed.for many coastal regions of the world, it has been common practice to apply seaweed to the land as a soil improver and fertilizer. seaweed is rich in arsenosugars and has a tissue concentration of arsenic up to 100 micro/g g(-1). these arsenic species are relatively nontoxic to humans; however, in the environment they may accumulate in the soil and decompose to more toxic arsenic species. the aim of this study was to determine the fate and biotransformation of these arsenosugars in soil using hp ...200312666926
effects of temperature on the production of hydrogen peroxide and volatile halocarbons by brackish-water algae.marine algae produce volatile halocarbons, which have an ozone-depleting potential. the formation of these compounds is thought to be related to oxidative stress, involving h2o2 and algal peroxidases. in our study we found strong correlations between the releases of h2o2 and brominated and some iodinated compounds to the seawater medium, but no such correlation was found for chcl3, suggesting the involvement of other formation mechanisms as well. little is known about the effects of environmenta ...200313679095
galactolipids rather than phlorotannins as herbivore deterrents in the brown seaweed fucus vesiculosus.the first investigation of seaweed chemical defense against herbivores involved the brown seaweed fucus vesiculosus and suggested defense via phlorotannins. the first demonstration of seaweed induction of secondary metabolites in response to herbivory also involved the genus fucus and assumed a defensive function for phlorotannins. many other investigations correlate herbivore feeding preference with changing levels of phlorotannins in this genus and others, but few directly test the effects of ...200312684854
inhibitory effect of fucoidan on the activities of crotaline snake venom myotoxic phospholipases a(2).myotoxic phospholipases a(2) account for most of the muscle necrosis that results from envenenomation by crotaline snakes. in this study, we investigated the protective effect of fucoidan, a natural sulfated polysaccharide obtained from the brown seaweed fucus vesiculosus, against the cytotoxic and myotoxic activities of a group of phospholipase a(2) myotoxins from crotaline snake venoms: bothrops asper myotoxins i, ii, iii, and iv, cerrophidion godmani myotoxins i and ii, atropoides nummifer my ...200314599557
a new antioxidant isobenzofuranone derivative from the algicolous marine fungus epicoccum sp.the fungus epicoccum sp., was isolated from the marine brown alga fucus vesiculosus. after cultivation the fungus was investigated for its secondary metabolite content, and found to contain the new natural product 4,5,6-trihydroxy-7-methylphthalide (1, epicoccone), together with 5-(acetoxymethyl)-furan-2-carboxylic acid (2), furan-2-carboxylic acid (3), 5-(hydroxymethyl)-furan-2-carboxylic acid (4), (-)-(3 r,4 s)-4-hydroxymellein (5), and (-)-(3 r)-5-hydroxymellein (6). the structures of all com ...200314598209
geostatistical analysis as applied to two environmental radiometric time series.this article details the results of an investigation into the application of geostatistical data analysis to two environmental radiometric time series. the data series employed consist of 99tc values for seaweed (fucus vesiculosus) and seawater samples taken as part of a marine monitoring program conducted on the coast of northern norway by the norwegian radiation protection authority. geostatistical methods were selected in order to provide information on values of the variables at unsampled ti ...200312666718
isolation and characterization of a fucoidan-degrading marine bacterium.fucoidan, a mixture of sulfated fucose-containing polysaccharides, was prepared from the algal bodies of cladosiphon okamuranus (class phaeophyceae, order chordariales, family chordariaceae) with a yield of 2.0% of the wet weight of the alga. to obtain enzymes that digest the fucoidan, we screened bacteria in the guy contents of the sea cucumber stichopus japonicus for their ability to decrease the fucoidan in their culture media, and successfully isolated one bacterial strain that could decreas ...200314735911
long-term study of 99tc in the marine environment on the swedish west coast.the activity concentration of (99)tc in brown seaweed (fucus vesiculosus and fucus serratus) and seawater were analysed in samples collected in 1991, 1995 and 2001 at several stations along the swedish west coast. in addition to these locations, a well-defined site (särdal, 56.76 degrees n, 12.63 degrees e) was included with (99)tc activity concentration data in seaweed from 1967 to 2000. over the years, the major source of (99)tc in the coastal waters of western sweden has been the radioactive ...200312660046
assessment of the effect of fucus vesiculosus extract on the labeling of blood constituents with technetium-99m and the histological modifications on the shape of the red blood cells.natural products are widely used as food or food additives or medicines for humans. we are trying to develop a model to assess the possible toxic properties of natural products, such as fucus vesiculosus, utilized in popular medicine. red blood cells (rbc) labeled with technetium-99m (99mtc) are used in various procedures in nuclear medicine. this labeling procedure depends on a reducing agent, and stannous chloride is used. there is evidence that this labeling may be altered by drugs. we have i ...200312453723
sea-to-land transfer of technetium-99 through the use of contaminated seaweed as an agricultural soil conditioner.the use of seaweed as an agricultural soil conditioner gives rise to a potential pathway for the transfer of technetium-99 ((99)tc) from marine to terrestrial ecosystems and thence to man. however, to date there is little information on the extent of the release of (99)tc from seaweed into soil and the mechanisms involved. this pot experiment has shown that (99)tc is released fairly rapidly from fucus vesiculosus into a sandy coastal soil. despite low temperature conditions, 60% of the (99)tc ad ...200312915065
glutathione-s-transferase activity of fucus spp. as a biomarker of environmental contamination.coastal zones are important areas from both ecological and economical points of view. however, in the last decades, in several regions of the globe, they have been increasingly impacted by complex discharges of contaminants and by marine traffic accidents. the portuguese atlantic coast is particularly exposed to these contaminants due to the proximity of important navigation routes. several rocky shore organisms have been tested and used as bioindicators of environmental contamination. however, ...200415588640
modelling 99tc concentrations in fucus vesiculosus from the north-east irish sea.in 1994 there were substantial increases in the quantity of 99tc discharged into the north-east irish sea from bnfl sellafield (uk), concomitant with improvements in waste treatment procedures. as a consequence, the concentration of 99tc observed in seawater and biota samples, taken from the irish sea coastline, increased significantly. elevated concentrations were also reported in dutch, danish, norwegian, swedish and arctic waters in subsequent years. in the present study a simple numerical mo ...200415312701
variation in natural selection for growth and phlorotannins in the brown alga fucus vesiculosus.directional selection for plant traits associated with resistance to herbivory tends to eliminate genetic variation in such traits. on the other hand, balancing selection arising from trade-offs between resistance and growth or spatially variable selection acts against the elimination of genetic variation. we explore both the amount of genetic variation and variability of natural selection for growth and concentration of phenolic secondary compounds, phlorotannins, in the brown alga fucus vesicu ...200415271080
zinc concentrations in marine macroalgae and a lichen from western ireland in relation to phylogenetic grouping, habitat and morphology.zinc concentrations in 19 species of marine macroalgae and a lichen from western ireland (spiddal, co. galway) were analysed by atomic absorption spectrophotometry. algae were collected from a single site but occupied different shore levels and belonged to distinct phylogenetic groupings and to different (previously recognised) morphological groups. concentrations ranged from 15-115 microg g(-1) dry weight. the greatest variation in concentration occurred amongst the red algal species, containin ...200415111037
arsenic binding to fucus vesiculosus metallothionein.the seaweed fucus vesiculosus is a member of the brown algae family. kille and co-workers [biochem. j. 338 (1999) 553] reported that this species contains the gene for metallothionein. metallothionein is a metalloprotein having low molecular weight, and high cysteine content, which binds a range of metals. f. vesiculosus bioaccumulates arsenic from the aquatic environment [mar. chem. 18 (1986) 321]. in this paper we describe arsenic binding to f. vesiculosus metallothionein, characterized by ele ...200415464992
the effect of fucus vesiculosus, an edible brown seaweed, upon menstrual cycle length and hormonal status in three pre-menopausal women: a case report.rates of estrogen-dependent cancers are among the highest in western countries and lower in the east. these variations may be attributable to differences in dietary exposures such as higher seaweed consumption among asian populations. the edible brown kelp, fucus vesiculosus (bladderwrack), as well as other brown kelp species, lower plasma cholesterol levels. since cholesterol is a precursor to sex hormone biosynthesis, kelp consumption may alter circulating sex hormone levels and menstrual cycl ...200415294021
analysis of bioactive ingredients in the brown alga fucus vesiculosus by capillary electrophoresis and neutron activation analysis.two different types of bioactive components of the seaweed fucus vesiculosus were analysed: (1) polyphenols (phlorotannins) by capillary electrophoresis (ce) and (2) mineral part (including bioactive microelements) by neutron activation analysis (naa). ce experiments were carried out using a uv detector (at 210 nm) and an uncoated silica capillary. the best separation was achieved at a voltage of 20 kv using borate or acetate buffer in a methanol/acetonitrile mixture as background electrolyte. t ...200415221191
genetic and environmental variation in performance of a marine isopod: effects of eutrophication.environmental variation in food resources modifies performance of herbivores, in addition to genetic variation and maternal effects. in marine benthic habitats, eutrophication may modify herbivores' diets by changing host species composition or nutritional quality of algae for herbivores. we studied experimentally the effects of diet breadth and nutrient availability for the host algae on fitness components of the herbivorous isopod idotea baltica. we fed the adult isopods with the brown algae f ...200415146322
intriguing asexual life in marginal populations of the brown seaweed fucus vesiculosus.reproduction of attached large brown algae is known to occur only by sexual zygotes. using microsatellites we show evolution of asexual reproduction in the bladder wrack promoting population persistence in the brackish water baltic sea (< 6 psu). here a dwarf morph of fucus vesiculosus is dominated by a single clone but clonal reproduction is also present in the common form of the species. we describe a possible mechanism for vegetative reproduction of attached algae, and conclude that clonality ...200515660953
brown kelp modulates endocrine hormones in female sprague-dawley rats and in human luteinized granulosa cells.epidemiological studies suggest that populations consuming typical asian diets have a lower incidence of hormone-dependent cancers than populations consuming western diets. these dietary differences have been mainly attributed to higher soy intakes among asians. however, studies from our laboratory suggest that the anti-estrogenic effects of dietary kelp also may contribute to these reduced cancer rates. as a follow-up to previous findings of endocrine modulation related to kelp ingestion in a p ...200515671230
effects of sedimentation on macroalgae: species-specific responses are related to reproductive traits.although increases in sedimentation have been proposed to interfere with benthic communities in many coastal areas worldwide, few experimental studies have investigated the effect of sedimentation on community composition and assessed species-specific responses. in a field experiment on a rocky shore on the swedish east coast (northern baltic proper) we confirmed the hypotheses that ambient sedimentation influences macroalgal abundance and community composition, and that species-specific respons ...200515682344
sulfated polysaccharides derived from dietary seaweeds increase the esterase activity of a lymphocyte tryptase, granzyme a.intake of sulfated polysaccharides, such as fucoidan or lambda-carrageenan extracted from seaweeds, has been shown to enhance immune responses, resulting in inhibition of tumor growth. however, little is known about the mechanisms by which these sulfated compounds mediate the enhancement. in the present study, we examined the effect of sulfated polysaccharides from seaweeds on esterase activity of a lymphocyte tryptase, granzyme a (gzma), which is believed to induce the production of cytokines i ...200516521710
genetic entities and mating system in hermaphroditic fucus spiralis and its close dioecious relative f. vesiculosus (fucaceae, phaeophyceae).to date, molecular markers have not settled the question of the specific status of the closely related, but phylogenetically unresolved, brown seaweeds, hermaphroditic fucus spiralis and dioecious fucus vesiculosus, nor their propensity for natural hybridization. to test the degree of species integrity and to assess effect of the mating system on the population genetic structure, 288 individuals coming from parapatric (discontinuous) and sympatric (contiguous) spatial configurations at two sites ...200515910325
studies of np and pu in the marine environment of swedish-danish waters and the north atlantic ocean.the long-lived anthropogenic radionuclides (237)np, (239)pu and (240)pu were determined in marine environmental samples (seaweed and seawater) collected from swedish-danish waters and the north atlantic ocean at various locations on different occasions during the period 1991-2001. the measurements were performed with sector field inductively coupled plasma mass spectrometry (icp-ms) and conventional alpha spectrometry. the (237)np activity concentrations in fucus vesiculosus and surface seawater ...200515885376
tracing the influence of sewage nitrogen in a coastal ecosystem using stable nitrogen isotopes.this paper reviews the use of stable nitrogen isotopes (delta15n) to delineate the influence of sewage nitrogen (n) in coastal ecosystems, drawing extensively on the case of himmerfjärden, a baltic sea bay that receives 15n-enriched tertiary treated sewage that is discharged mainly as dissolved inorganic n (din). gradients of delta15n in macroalgae (fucus vesiculosus) and surface sediments traced sewage-derived n to 24 km from the outfall but elevated delta15n values (> 7 per thousand) indicated ...200515865312
contents of soluble, cell-wall-bound and exuded phlorotannins in the brown alga fucus vesiculosus, with implications on their ecological functions.phlorotannins are ubiquitous secondary metabolites in brown algae that are phenotypically plastic and suggested to have multiple ecological roles. traditionally, phlorotannins have been quantified as total soluble phlorotannins. here, we modify a quantification procedure to measure, for the first time, the amount of cell-wall-bound phlorotannins. we also optimize the quantification of soluble phlorotannins. we use these methods to study the responses of soluble and cell-wall-bound phlorotannin t ...200515839490
genotypic variation in tolerance and resistance to fouling in the brown alga fucus vesiculosus.in this study, we examined genetic variation in resistance and tolerance to fouling organisms in the brown alga fucus vesiculosus. we first grew 30 algal genotypes in the field, where we allowed fouling organisms to colonise the genotypes at natural levels. we then conducted a manipulative experiment, where we grew 20 genotypes of algae in aquaria with or without fouling organisms. we measured host resistance as the load of fouling organisms and tolerance as the slope of the regression of algal ...200515800737
renal peroxidative changes mediated by oxalate: the protective role of fucoidan.oxalate, one of the major constituents of renal stones is known to induce free radicals which damage the renal membrane. damaged epithelia might act as nidi for stone formation aggravating calcium oxalate precipitation during hyperoxaluria. in the present study, the beneficial effects of fucoidan on oxalate-induced free radical injury were investigated. male wistar rats were divided into four groups. hyperoxaluria was induced in two groups by administration of 0.75% ethylene glycol in drinking w ...200616820173
convergent adaptation to a marginal habitat by homoploid hybrids and polyploid ecads in the seaweed genus fucus.hybridization and polyploidy are two major sources of genetic variability that can lead to adaptation in new habitats. most species of the brown algal genus fucus are found along wave-swept rocky shores of the northern hemisphere, but some species have adapted to brackish and salt marsh habitats. using five microsatellite loci and mtdna rflp, we characterize two populations of morphologically similar, muscoides-like fucus inhabiting salt marshes in iceland and ireland. the icelandic genotypes we ...200617148415
determination of the cd/s cluster stoichiometry in fucus vesiculosus metallothionein.the seaweed fucus vesiculosus is unusual when compared with other algal species, in that it can survive in toxic-metal-contaminated aquatic environments. the metallothionein gene has been identified in f. vesiculosus by kille and co-workers (morris, c. a., nicolaus, b., sampson, v., harwood, j. l., and kille, p. (1999) biochem. j. 338, 553), which suggests a possible protective mechanism against toxic metals for this species. we report the first detailed study of the metal binding properties of ...200616544940
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