Publications

spotted fever group rickettsiae in questing ticks, central spain.		0	23763913
migratory birds, ticks, and crimean-congo hemorrhagic fever virus.		0	23171591
rickettsia massiliae in the canary islands.		0	22531111
development of hyalomma lusitanicum under laboratory conditions.	at the constant temperature of 25 degrees c and relative humidity (rh) of 84%, the average pre-oviposition period of hyalomma lusitanicum was 47 days, the oviposition lasted an average of 26 days and the total egg production was an average of 6320 per female. at 16 degrees c the females did not lay eggs at all, but those which survived for 1 year and were transferred thereafter to 25 degrees c and 84% rh laid viable eggs. at 35 degrees c, the oviposition was identical at all levels of rh tested ...	1984	6541395
[scanning electron microscopy study for an analytical key to the hyalomminae (ixodidae) species to be found in italy].	identification of ticks of hyalomminae subfamily is easy for the species of subgenus hyalommasta schulze (coxa l with two equal, stump and short, well separated spurs) and for those of the subgenera hyalommina schulze and delpyella santos dias (basis capituli with lateral margins anteriorly divergent). all the species of subgenus hyalomma koch have the coxa i deeply divided in adjacent narrow external spur and wider internal spur, and the basis capituli rectangular, with parallel or subparallel ...	1996	9333752
18s rrna gene sequences and phylogenetic relationships of european hard-tick species (acari: ixodidae).	the complete 18s rrna gene sequences of the following six european hard-tick species were obtained by direct pcr cycle sequencing and silver-staining methods: rhipicephalus pusillus, boophilus annulatus, dermacentor marginatus, hyalomma lusitanicum, haemaphysalis punctata, and ixodes ricinus. differences observed in the sequence alignment of these six species together with the 18s rrna gene sequences of 13 other hard-tick species demonstrate that this gene is a good marker for supraspecific diff ...	1998	9491423
mitochondrial 16s rdna sequences and phylogenetic relationships of species of rhipicephalus and other tick genera among metastriata (acari: ixodidae).	the mitochondrial 16s rrna gene sequences of the following eight european metastriata tick species were obtained by direct polymerase-chain-reaction cycle sequencing and silver-staining methods: rhipicephalus bursa, r. pusillus, r. sanguineus, r. turanicus, boophilus annulatus, dermacentor marginatus, haemaphysalis punctata, and hyalomma lusitanicum. this mitochondrial gene seems to be a good marker for the establishment of genetic relationships among closely related tick species, but it does no ...	1998	9660138
general review of tick species present in portugal.	at present, 24 species are known to occur in portugal: argas vespertilionis, ornithodoros maritimus and ornithodoros erraticus in argasidae; ixodes acuminatus, ixodes bivari, ixodes canisuga, ixodes frontalis, ixodes hexagonus, ixodes ricinus, ixodes simplex simplex, ixodes ventalloi, ixodes vespertilionis, dermacentor marginatus, dermacentor pictus', haemaphysalis hispanica, haemaphysalis inermis, haemaphysalis punctata, rhipicephalus bursa, rhipicephalus pusillus, rhipicephalus sanguineus, rhi ...	1999	11071535
studies on theileriosis in southern spain.	mediterranean theileriosis is widely distributed throughout southern spain, where it causes economic losses of considerable importance. in recent studies on 221 fighting bulls from 29 locations of different provinces, mainly in southern spain, the seroprevalence was 75.1% by ifat. on the other hand, we are carrying out studies on beef and dairy breeds from eight provinces in the southern half of spain where the positivity by ifat ranges from 50 to more than 90% of the sampled cattle. studies on ...	1999	11071556
epidemiology of mediterranean theileriosis in extremadura region, spain.	extremadura is an extensive region (41,000 km2) located in the southwest of spain, where animal husbandry is a very important economical resource. livestock parasitism of ticks is common in the region and it is favoured by the environmental conditions and management systems. mediterranean theileriosis (mt) is perhaps the most important tick-borne disease of cattle. although theileria annulata has been identified in extremadura since 1933, little epidemiological information is available. during t ...	1999	11071543
studies on the transmission of theileria annulata to cattle by the tick hyalomma lusitanicum.	the role of the ixodid tick hyalomma lusitanicum koch 1844 as a vector of mediterranean or tropical theileriosis (caused by the protozoan parasite theileria annulata dschunkowsky et luhs 1904) in southern spain was studied. hyalomma lusitanicum was the most common tick, and the only species of the genus hyalomma l., found on t. annulata-infected cattle from the theileriosis enzootic area studied (province of cádiz, southern spain). likewise, we found that all sera of the cattle previously consid ...	1999	10574066
studies on theileria and babesia infecting live and slaughtered animals in al arish and el hasanah, north sinai governorate, egypt.	during the year 2001, a total of 475 sheep, 200 goats, 135 cattle and 190 camels in el arich city and el hassanah center were examined for babesia ovis and theileria ovis. blood films were taken from the vein of the ear. meanwhile, the animals were examined for tick infestations. b. ovis and th. ovis were detected in 13 (2.7%), and 14 (2.9%) sheep, 14 (7.0%), and 15 (7.5%) goats, 13 (9.6%), and 11 (8.1%) cattle and 18 (9.5%), and 24 (12.6%) camels respectively. on the other hand, double infectio ...	2002	12214937
ectoparasite ticks and chewing lice of red-legged partridge, alectoris rufa, in spain.	during the 1992-1993 and 1993-1994 shooting seasons, 212 wild red-legged partridge, alectoris rufa (galliformes: phaisanide) were captured in 18 spanish provinces and examined for chewing lice and ticks. three tick species and six species of chewing lice were found. ticks (acari: ixodidae) haemaphysalis punctata canestrini & fanzago, hyalomma lusitanicum koch and ixodes frontalis panzer were found at the lowest prevalence (1.4% overall tick prevalence). the louse (mallophaga) species, goniodes d ...	2003	12680922
ticks infesting livestock on farms in western sicily, italy.	the results of a 2-year survey on ticks infesting livestock on farms in western sicily, italy, are reported. several dogs living in the same area were also examined for ticks. moreover, free-living ticks from one cattle farm were caught by co(2) traps and flagging. a total of 6208 specimens was collected belonging to 9 species: rhipicephalus bursa (32.4%), rhipicephalus turanicus (22.7%), rhipicephalus sanguineus (19.3%), hyalomma lusitanicum (12.0%), haemaphysalis punctata (7.8%), hyalomma marg ...	2006	16550337
molecular diagnosis of theileria and babesia species infecting cattle in northern spain using reverse line blot macroarrays.	piroplasmosis in cattle is caused by tick-borne haemoprotozoan parasites of the genera theileria and babesia. molecular detection techniques offer higher sensitivity and specificity than microscopy examination methods and serological tests. a reverse line blot (rlb) macroarray that included generic and species-specific probes for theileria annulata, theileria buffeli, babesia bovis, babesia bigemina, babesia divergens and babesia major was used to study the presence and identity of the piroplasm ...	2006	16684356
rickettsia sibirica isolation from a patient and detection in ticks, portugal.	we report the first isolation of rickettsia sibirica (strain mongolotimonae) from the blood of a patient and detection by polymerase chain reaction (pcr) of the rickettsia in a rhipicephalus pusillus tick collected from a dead mongoose (herpestes ichneumon) in the alentejo region, portugal. we describe also the first pcr detection of a new rickettsia strain that is related to r. sibirica.	2006	16836827
toxicity of oxalic acid against adult hyalomma lusitanicum ticks (ixodida: ixodidae) in laboratory conditions: ld50.	the abundance of hyalomma lusitanicum ticks in open areas of central spain is the result of many natural and human factors. control of tick populations by chemical and other means may be necessary until we can determine the key global factors in this important tick population. despite many attempts to establish ecological control of ticks, there is little data about the activity of organic acids. this article describes tests on the in vitro toxicity of oxalic acid (oa) against wild h. lusitanicu ...	2008	18714872
spotted fever group rickettsia in ticks from southeastern spain natural parks.	during an 8-years study, we collected from vegetation or domestic and wild mammals 1246 ticks (624 males, 511 females and 111 nymphs) belonging to 13 species in jaen province (andalusia) and we analyzed these ticks by pcr and sequencing for the presence of rickettsiae. specific rickettsiae dna was detected in 243 (19.5%) of the ticks tested. sequence analysis of amplicons of glta, ompa and ompb genes revealed that ixodes ricinus were infected with r. monacensis, including strain irs3, and r. hel ...	2008	18677442
some hydrolase activities from the tick hyalomma lusitanicum koch, 1844 (ixodoidea: ixodida).	in this work has been made a detection and preliminary characterization of some hydrolases in whole extracts from unfed adult males and females of hyalomma lusitanicum, one of the vectors for theileria annulata that causes mediterranean theileriosis in cattle. we have elected as targets, proteases as enzymes implicated in the nutritional processes of ticks, esterases that are usually implicated in resistance to organophosphates and phosphatises often implicated in protein phosphorilation and con ...	2008	19202766
detection of coxiella burnetii in ticks collected from central spain.	a total of 1482 adult ticks collected from vegetation and animals in central spain in 2003-2005 were tested for the presence of coxiella burnetii by polymerase chain reaction and subsequent reverse line blot hybridization (pcr-rlb). c. burnetii was identified in 7.7% of questing ticks (80/1039) and 3.4% of ticks collected from animals (15/443) belonging to four species: hyalomma lusitanicum, dermacentor marginatus, rhiphicephalus sanguineus, and r. pusillus. these findings show an active role of ...	2009	18945192
tick-borne zoonotic bacteria in ticks collected from central spain.	the prevalence of tick-borne and related bacteria infecting adult ticks in central spain was assessed by molecular methods. six areas were sampled monthly during a 2-year longitudinal study. a total of 1,038 questing and 442 feeding ticks, belonging to eight different species, were tested. the most abundant species were hyalomma lusitanicum (54% of captures), followed by dermacentor marginatus (23%) and rhipicephalus sanguineus (10%). four human pathogens, including seven rickettsia species, ana ...	2009	19556569
prevalence of tick-borne pathogens in ticks in sicily.	the prevalence of anaplasma, ehrlichia, rickettsia and babesia/theileria species was analysed in questing and feeding adult ticks in sicily. a total of 678 ticks were collected and analysed in this study. of these, 29 were questing ticks and 649 were collected from infested cattle, sheep, goats or dogs. tick species analysed included rhipicephalus bursa, r. turanicus, r. sanguineus, hyalomma lusitanicum, h. marginatum, dermacentor marginatus, ixodes ricinus, r. (boophilus) annulatus and haemaphy ...	2010	20537102
subolesin expression in response to pathogen infection in ticks.	ticks (acari: ixodidae) are vectors of pathogens worldwide that cause diseases in humans and animals. ticks and pathogens have co-evolved molecular mechanisms that contribute to their mutual development and survival. subolesin was discovered as a tick protective antigen and was subsequently shown to be similar in structure and function to akirins, an evolutionarily conserved group of proteins in insects and vertebrates that controls nf-kb-dependent and independent expression of innate immune res ...	2010	20170494
coinfections of rickettsia slovaca and rickettsia helvetica with borrelia lusitaniae in ticks collected in a safari park, portugal.	borrelia and rickettsia bacteria are the most important tick-borne agents causing disease in portugal. identification and characterization of these circulating agents, mainly in recreational areas, is crucial for the development of preventive measures in response to the gradually increasing exposure of humans to tick vectors. a total of 677 questing ticks including dermacentor marginatus, rhipicephalus sanguineus, ixodes ricinus, hyalomma lusitanicum, h. marginatum, and haemaphysalis punctata we ...	2010	21771525
biogeography of tick-borne bhanja virus (bunyaviridae) in europe.	bhanja virus (bhav) is pathogenic for young domestic ruminants and also for humans, causing fever and affections of the central nervous system. this generally neglected arbovirus of the family bunyaviridae is transmitted by metastriate ticks of the genera haemaphysalis, dermacentor, hyalomma, rhipicephalus, boophilus, and amblyomma. geographic distribution of bhav covers southern and central asia, africa, and southern (partially also central) europe. comparative biogeographic study of eight know ...	2010	20182535
the hard-tick fauna of mainland portugal (acari: ixodidae): an update on geographical distribution and known associations with hosts and pathogens.	this work is an updated revision of the available information on portuguese ixodid tick species. it includes data on tick biology, ecology, taxonomy and host/pathogen-associations. the current list of portuguese ixodid ticks comprises twenty species: dermacentor marginatus (sulzer, 1776), dermacentor reticulatus (fabricius, 1794), haemaphysalis hispanica gil collado, 1938, haemaphysalis inermis birula, 1895, haemaphysalis punctata canestrini & fanzago, 1878, hyalomma lusitanicum koch, 1844, hyal ...	2011	21452063
differences in questing tick species distribution between atlantic and continental climate regions in spain.	climate and vegetation in spain vary from north to south, affecting tick distribution and consequently the presence of tick-borne diseases. the aim of this study was to investigate throughout a 2-yr study the distribution of the different exophilic questing tick species present in 18 areas: eight located in central and 10 in northern spain. the same methodology was used in both areas, sampling vegetation on a monthly basis by blanket dragging for 20- to 30-min intervals. a total of 12 species be ...	2011	21337943
growth of coxiella burnetii in the ixodes scapularis-derived ide8 tick cell line.	q fever, a zoonotic disease, is caused by a gram-negative intracellular bacterium, coxiella burnetii. although normally transmitted during exposure to infectious aerosols, c. burnetii is also found in arthropod vectors. in the environment, ticks are thought to play a crucial role in bacterial maintenance and transmission by infecting various mammalian species. however, the nature of the pathogen-tick relationship is not well defined. to determine c. burnetii's interactions with a cultured tick c ...	2011	21254834
development and evaluation of a real-time pcr assay for the quantitative detection of theileria annulata in cattle.	the tick-borne apicomplexan bovine parasite theileria annulata is endemic in many tropical and temperate areas, including minorca (balearic islands, spain). real-time pcr is widely used for the detection of piroplasms but quantification is not commonly considered.	2012	22889141
association of environmental traits with the geographic ranges of ticks (acari: ixodidae) of medical and veterinary importance in the western palearctic. a digital data set.	we compiled information on the distribution of ticks in the western palearctic (11°w, 45°e; 29°n, 71°n), published during 1970-2010. the literature search was filtered by the tick's species name and an unambiguous reference to the point of capture. records from some curated collections were included. we focused on tick species of importance to human and animal health, in particular: ixodes ricinus, dermacentor marginatus, d. reticulatus, haemaphysalis punctata, h. sulcata, hyalomma marginatum, h ...	2012	22843316
zoonotic pathogens associated with hyalomma aegyptium in endangered tortoises: evidence for host-switching behaviour in ticks?	hyalomma aegyptium is a hard-tick with a typical three-host life cycle. the main hosts are palearctic tortoises of genus testudo. however, other hosts can be used by immature ticks for feeding in natural conditions. given this complex ecology and multiple host use, the circulation of pathogens by h. aegyptium between various hosts can be important from epidemiological point of view. the aim of this study was to evaluate the role of h. aegyptium as natural carrier of four important zoonotic patho ...	2012	23273169
coevolutionary analyses of the relationships between piroplasmids and their hard tick hosts.	host-parasite coevolution is a key driver of biological diversity. to examine the evolutionary relationships between piroplasmids and their hard tick hosts, we calculated the molecular clock and conducted phylogenetic analyses of both groups. based on our results, we conclude that the divergence time of piroplasmids (∼56 mya) is later than divergence time of their hard tick hosts (∼86 mya). from analyses of the evolution of both piroplasmid and vector lineages and their association, we know that ...	2013	24101988
sex-biased differences in the effects of host individual, host population and environmental traits driving tick parasitism in red deer.	the interactions between host individual, host population, and environmental factors modulate parasite abundance in a given host population. since adult exophilic ticks are highly aggregated in red deer (cervus elaphus) and this ungulate exhibits significant sexual size dimorphism, life history traits and segregation, we hypothesized that tick parasitism on males and hinds would be differentially influenced by each of these factors. to test the hypothesis, ticks from 306 red deer-182 males and 1 ...	2013	23819112
isolation and characterization of babesia pecorum sp. nov. from farmed red deer (cervus elaphus).	the diversity of babesia species infecting cervids in parts of central and southern spain was analyzed by collecting blood from farmed red deer (cervus elaphus). babesia sp. was isolated in vitro from two red deer herds in cádiz and ciudad real. the number of babesia sp. carriers differed between the two herds: 36/77 in cádiz and 1/35 in ciudad real. hyalomma lusitanicum was the most prevalent tick species identified on the cádiz farm vegetation and on sampled animals, and is therefore a candida ...	2014	25155988
ticks infesting humans in italy and associated pathogens.	ticks may transmit a large variety of pathogens, which cause illnesses in animals and humans, commonly referred to as to tick-borne diseases (tbds). the incidence of human tbds in italy is underestimated because of poor surveillance and the scant amount of studies available.	2014	25023709
control of tick infestations and pathogen prevalence in cattle and sheep farms vaccinated with the recombinant subolesin-major surface protein 1a chimeric antigen.	despite the use of chemical acaricides, tick infestations continue to affect animal health and production worldwide. tick vaccines have been proposed as a cost-effective and environmentally friendly alternative for tick control. vaccination with the candidate tick protective antigen, subolesin (sub), has been shown experimentally to be effective in controlling vector infestations and pathogen infection. furthermore, escherichia coli membranes containing the chimeric antigen composed of sub fused ...	2014	24398155
tick-borne infections in human and animal population worldwide.	the abundance and activity of ectoparasites and its hosts are affected by various abiotic factors, such as climate and other organisms (predators, pathogens and competitors) presenting thus multiples forms of association (obligate to facultative, permanent to intermittent and superficial to subcutaneous) developed during long co-evolving processes. ticks are ectoparasites widespread globally and its eco epidemiology are closely related to the environmental conditions. they are obligatory hematop ...	2015	27047089
the recent evolution of a maternally-inherited endosymbiont of ticks led to the emergence of the q fever pathogen, coxiella burnetii.	q fever is a highly infectious disease with a worldwide distribution. its causative agent, the intracellular bacterium coxiella burnetii, infects a variety of vertebrate species, including humans. its evolutionary origin remains almost entirely unknown and uncertainty persists regarding the identity and lifestyle of its ancestors. a few tick species were recently found to harbor maternally-inherited coxiella-like organisms engaged in symbiotic interactions, but their relationships to the q fever ...	2015	25978383
climate suitability for european ticks: assessing species distribution models against null models and projection under ar5 climate.	there is increasing evidence that the geographic distribution of tick species is changing. whilst correlative species distribution models (sdms) have been used to predict areas that are potentially suitable for ticks, models have often been assessed without due consideration for spatial patterns in the data that may inflate the influence of predictor variables on species distributions. this study used null models to rigorously evaluate the role of climate and the potential for climate change to ...	2015	26310856
control of tick infestations in oryctolagus cuniculus (lagomorpha: leporidae) with spinosad under laboratory and field conditions.	because of great economic loss in the world's livestock industry, and the serious risks to human health, the control of ticks and tick-borne diseases is one of the most important health management issues today. current methodology involves integrated tick control for preventing the development of resistance. rabbits are hosts for immature stages of the three-host tick hyalomma lusitanicum koch; so, we focus on this host as a strategy to interrupt the tick life cycle. spinosad is an insecticide-a ...	2015	26336305
importation of a hyalomma lusitanicum tick into the uk on a dog.		2016	27770095
sero-epidemiological survey of crimean-congo hemorrhagic fever virus in tunisia.	crimean-congo hemorrhagic fever (cchf) is a tick-borne disease associated with a high case fatality rate and transmitted mainly by hyalomma marginatum. the geographical distribution of h. marginatum covers most of the western mediterranean basin. we aimed to investigate whether cchf virus (cchfv) is circulating in tunisia. samples from unexplained acute febrile patients (n = 181) and a high risk group of humans, mainly slaughter workers (n = 38), were collected in the summer of 2014 and analyzed ...	2016	26956221
detection of bartonella tamiae, coxiella burnetii and rickettsiae in arthropods and tissues from wild and domestic animals in northeastern algeria.	in recent years, the scope and importance of emergent vector-borne diseases has increased dramatically. in algeria, only limited information is currently available concerning the presence and prevalence of these zoonotic diseases. for this reason, we conducted a survey of hematophagous ectoparasites of domestic mammals and/or spleens of wild animals in el tarf and souk ahras, algeria.	2016	26791781
african swine fever virus transmission cycles in central europe: evaluation of wild boar-soft tick contacts through detection of antibodies against ornithodoros erraticus saliva antigen.	african swine fever (asf) is one of the most complex viral diseases affecting both domestic and wild pigs. it is caused by asf virus (asfv), the only dna virus which can be efficiently transmitted by an arthropod vector, soft ticks of the genus ornithodoros. these ticks can be part of asfv-transmission cycles, and in europe, o. erraticus was shown to be responsible for long-term maintenance of asfv in spain and portugal. in 2014, the disease has been reintroduced into the european union, affecti ...	2016	26728767
control of hyalomma lusitanicum (acari: ixodidade) ticks infesting oryctolagus cuniculus (lagomorpha: leporidae) using the entomopathogenic fungus beauveria bassiana (hyocreales: clavicipitaceae) in field conditions.	entomopathogenic fungi are widely used to control arthropods not just in agricultural settings but also in veterinary medicine and public health. these products have been employed to control tick populations and tick-borne diseases. the effectiveness of these control measures not only depends on the fungi, but also on the tick species and environmental conditions. in mesomediterranean areas, tick species are adapted to extreme climatic conditions and it is therefore especially important to devel ...	2016	27297213
long term study of ixodid ticks feeding on red deer (cervus elaphus) in a meso-mediterranean climate.	red deer (cervus elaphus l.) are very valuable in trophy-hunting but also contribute to the preservation of natural areas. they are affected by many parasites and pathogens, including hard ticks that are not only important parasites themselves but can also act as vectors and/or reservoirs of pathogens. tick phenology is complex insofar as population dynamics depend on environmental conditions, vegetation, host availability and their own intrinsic characteristic. ticks were collected monthly from ...	2016	26715543
long-term ecological study of host-seeking adults of hyalomma lusitanicum (acari: ixodidae) in a meso-mediterranean climate.	from january 2007 to december 2014, three representative meso-mediterranean bioclimatic environment types were sampled monthly using blanket-dragging techniques to determine the tick abundance rate. hyalomma lusitanicum koch, 1844 was the most prevalent species (96.58%) followed by dermacentor marginatus sulzer, 1776; rhipicephalus pusillus gil collado, 1936; and rhipicephalus bursa canestrini and fanzago, 1878. h. lusitanicum adults begin questing activity around march, numbers rising quickly r ...	2016	26477051
seasonal dynamics of ixodid ticks on wild rabbits oryctolagus cuniculus (leporidae) from central spain.	rabbit hemorrhagic disease and myxomatosis caused a decline in the rabbit population in the second half of the last century. despite some recovery, the risk of vector-borne disease is present and thus the importance of controlling vector populations. in the current study, we describe the ixodid tick fauna in wild rabbit in a natural reserve in ciudad real (central spain) during the course of two 3-year periods (2007-2009 and 2012-2014). of all the ticks collected on average 72.5 % were larvae, 2 ...	2016	27411939
a comparative test of ixodid tick identification by a network of european researchers.	this study reports the results of a comparative test of identification of ticks occurring in western europe and northern africa. a total of 14 laboratories were voluntarily enrolled in the test. each participant received between 22 and 25 specimens of adult and nymphal ticks of 11 species: dermacentor marginatus, d. reticulatus, haemaphysalis punctata, hyalomma lusitanicum, hy. marginatum, ixodes ricinus, i. hexagonus, rhipicephalus annulatus, r. bursa, r. rossicus, and/or r. sanguineus s.l. tic ...	2017	28320640
tick-borne bacteria and protozoa detected in ticks collected from domestic animals and wildlife in central and southern portugal.	ticks are vectors of many human and animal pathogens. the aim of this study was to screen bacteria and protozoa from ticks infesting domestic animals and wildlife collected in central and southern portugal. a total of 593 ticks, comprising 465 (78.4%) adults, 122 (20.6%) nymphs, and six (1.0%) larvae, were collected from 283 hosts of 25 different species (4 domestic and 21 wild). overall, the analysis of dna extracts prepared from ticks collected from hosts of 11 different species in the distric ...	2017	28958791
a retrospective study of the characterization of rickettsia species in ticks collected from humans.	rickettsiae (family rickettsiaceae, order rickettsiales) are obligate intracellular bacteria transmitted by arthropod vectors. several rickettsia species causing vector-borne rickettsioses belong to the spotted fever group (sfg). traditionally, rickettsia conorii has been considered as the main etiologic agent of mediterranean spotted fever. however, the molecular characterization of rickettsiae allowed identifying other species involved in spotted fever in the mediterranean region. in this stud ...	2017	28457821
genomic characterization of crimean-congo hemorrhagic fever virus in hyalomma tick from spain, 2014.	crimean-congo hemorrhagic fever (cchf) is a severe tick-borne disease caused by cchf virus (cchfv). ticks in the genus hyalomma are the main vectors and reservoirs of cchfv. in spain, cchfv was first detected in hyalomma ticks from cáceres in 2010. subsequently, two autochthonous cchf cases were reported in august 2016. in this study, we describe the characterization of the cchfv genome directly from hyalomma lusitanicum collected in cáceres in 2014. phylogenetic analyses reveal a close relation ...	2017	28836897
detection of coxiella burnetii dna in peridomestic and wild animals and ticks in an endemic region (canary islands, spain).	coxiella burnetii, the etiological agent of human q fever, can infect mammals, birds, and arthropods. the canary islands (spain) are considered an endemic territory, with a high prevalence in both humans and livestock. nonetheless, there is no epidemiological information about the wild and peridomestic cycles of c. burnetii. tissue samples from rodents on farms (100) and wild rabbits (129) were collected and assessed by pcr to detect c. burnetii dna. in parallel, ticks were also collected from v ...	2017	28759337
molecular (ticks) and serological (humans) study of crimean-congo hemorrhagic fever virus in the iberian peninsula, 2013-2015.	crimean-congo hemorrhagic fever (cchf) is a viral disease, mainly transmitted through tick bite, of great importance in public health. in spain, crimean-congo hemorrhagic fever virus (cchfv) was detected for the first time in 2010 in hyalomma lusitanicum ticks collected from deer in cáceres. the aim of this study was to investigate the presence of cchfv in ticks from cáceres, and from other spanish areas, and to evaluate the presence of antibodies against the virus in individuals exposed to tick ...	2017	28291670
pcr screening of tick-borne agents in sensitive conservation areas, southeast portugal.	the southeast region of portugal, particularly the guadiana valley, is currently the reintroduction territory of lynx pardinus (iberian lynx), one of the most endangered felids in the world that is only found in the iberian peninsula. over the last century, populations have declined, placing l. pardinus at extremely high risk of extinction in the wild and relying on reintroduction projects. among the aspects taken into account in the establishment of new populations is the sanitary status of the ...	2017	27894847
insect antifeedant and ixodicidal compounds from senecio adenotrichius.	nine eremophilane sesquiterpenes 1 - 9, two flavonoids 11 and 12, and two known pirrolizidine alkaloids 13 and 14, were isolated from senecio adenotrichius dc. their structures were elucidated on the basis of spectroscopic data and by comparison with previously reported spectroscopic data of similar compounds. compounds 5, 7, and 9 have not been previously reported as natural products. the antifeedant activity of these compounds was tested against spodoptera litoralis and myzus persicae. eremoph ...	2017	27546067
influence of environmental temperature and humidity on questing ticks in central spain.	ticks are important ectoparasites and vectors of the pathogens that cause disease in humans and animals. at a natural reserve in ciudad real (spain), an uncontrollable infestation of wild animals with hyalomma lusitanicum (koch) has been reported by some game reserve owners. many questions about distribution, abundance, and phenology of this tick in this area remain unanswered. the aim of this study was to determine if temperature and relative humidity affect the questing tick's activity in four ...	2017	28417247
the effect of excluding ungulates on the abundance of ixodid ticks on wild rabbit (oryctolagus cuniculus).	tick abundance depends heavily on host density, so reducing access to the host should reduce tick populations in a determined area. in this study we compared the parasitic index (pi = average number of ticks per rabbit) of two wild rabbit populations separated 16 years ago by a fence keeping ungulates from moving freely. two areas were selected (closed and open) wherein 50 wild rabbits per area were sampled for ticks. the pi in the open area (pi = 989.62) was significantly higher than in the clo ...	2017	28840404
in vitro feeding of hyalomma lusitanicum ticks on artificial membranes.	in vitro feeding of ticks (acari: ixodidae) is an important means to study the biology of ticks and their vectorial capacity. here, we have adapted the tick hyalomma lusitanicum koch to previously published silicone-based membranes for in vitro feeding. for comparison purposes data on pre-oviposition, oviposition and hatching from females engorged on animals were used. a total of 68 engorged females out of 169 were obtained; feeding duration and feeding behaviour were similar to that of ticks on ...	2017	28840366
biocidal potential and chemical composition of industrial essential oils from hyssopus officinalis, lavandula × intermedia var. super, and santolina chamaecyparissus.	this work presents the biocidal (insecticidal, ixodicidal, nematicidal, and phytotoxic) effects and chemical compositions of three essential oils obtained from the industrial steam distillation (ieos) of hyssop (hyssopus officinalis l.), lavandin (lavandula × intermedia or l. × hybrida var. super), and cotton lavender (santolina chamaecyparissus l.). their chemical composition analyzed by gas chromatography coupled to mass spectrometry showed 1,8-cineole (53%) and β-pinene (16%) as the major com ...	2018	28801935
potential risk posed by the importation of ticks into the uk on animals: records from the tick surveillance scheme.	in order to monitor important tick vectors in the uk, public health england's tick surveillance scheme (tss) receives specimens from across the country for identification. in recent years, an increasing number of these specimens have been removed from animals with a recent history of travel outside the uk. this paper presents all data collated by the tss on ticks entering the country on recently travelled or imported animals since surveillance commenced in 2005. ten different tick species repres ...	2018	29217768