Publications

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isolation and identification of an ice-nucleating bacterium from the gills of the intertidal bivalve mollusc geukensia demissa.in the fall, freeze tolerant intertidal invertebrates usually produce ice-nucleating proteins that are secreted into the hemolymph. these proteins help protect against freeze damage by insuring that ice formation is limited to extracellular spaces. geukensia demissa, a freeze tolerant, salt marsh bivalve mollusc was examined for the presence of ice nucleating proteins. the ice-nucleating temperature (int) of the hemolymph was not significantly different from artificial seawater of the same salin ...200111399277
recharacterization of pseudomonas fulva iizuka and komagata 1963, and proposals of pseudomonas parafulva sp. nov. and pseudomonas cremoricolorata sp. nov.seven pseudomonas fulva strains obtained from culture collections were taxonomically studied. the seven strains were separated into three clusters (clusters i to iii) on the basis of 16s rrna gene sequences, and located phylogenetically in the genus pseudomonas sensu stricto. further, the strains were classified into 4 groups (groups i to iv) on the basis of dna-dna similarity. as a result, cluster i was split into groups i and ii. group i included the type strain of p. fulva and two strains, an ...200112483612
pseudomonas pachastrellae sp. nov., isolated from a marine sponge.two gram-negative, non-fermentative, non-denitrifying, non-pigmented, rod-shaped bacteria that were motile by means of polar flagella, designated strains kmm 330(t) and kmm 331, were isolated from a deep-sea sponge specimen and subjected to a polyphasic taxonomic study. the new isolates exhibited 16s rrna gene sequence similarity of 99.9 %, and their mean level of dna-dna relatedness was 82 %. phylogenetic analysis based on their 16s rrna gene sequences placed the strains within the genus pseudo ...200515774686
characterization of bacillus and pseudomonas strains with suppressive traits isolated from tomato hydroponic-slow filtration unit.bacillus and pseudomonas spp. are known to be involved in plant pathogenic fungi elimination during the slow filtration process used in tomato soilless cultures. we isolated 6-8 strains of both bacillus and pseudomonas from the top, middle, and bottom sections of filters and identified them after 16s rdna sequencing. four pseudomonas strains were identified as pseudomonas fulva, 5 as pseudomonas plecoglossicida, and 12 as pseudomonas putida. the use of specific oligonucleotide polymerase chain r ...200717668039
first case of human infection due to pseudomonas fulva, an environmental bacterium isolated from cerebrospinal fluid.we report the first case of human infection due to pseudomonas fulva. p. fulva caused acute meningitis following the placement of a drainage system in a 2-year-old female. additionally, the isolate displayed a vim-2 carbapenemase in a class 1 integron context.201020032258
control of pyrimidine nucleotide formation in pseudomonas fulva.control of pyrimidine formation was examined in pseudomonas fulva atcc 31418. pyrimidine supplementation lowered pyrimidine biosynthetic pathway enzyme activities in cells grown on glucose or succinate as a carbon source indicating possible repression of enzyme synthesis. pyrimidine limitation experiments were conducted using an orotidine 50-monophosphate decarboxylase mutant strain isolated in this study. compared to uracil-supplemented, glucose-grown mutant cells, pyrimidine limitation of this ...201020066562
first report of bloodstream infection caused by pseudomonas fulva.pseudomonas fulva has not yet been isolated from humans as a pathogen. herein, we report the first case of p. fulva bacteremia in a patient hospitalized due to trauma. the species was identified using biochemical and molecular genetic analyses of the 16s rrna, gyrb, rpob, and rpod genes.201020444972
biosynthesis of medium-chain-length poly(3-hydroxyalkanoates) by volatile aromatic hydrocarbons-degrading pseudomonas fulva ty16.pseudomonas fulva ty16 biosynthesized medium-chain-length poly(3-hydroxyalkanoates) (mcl-phas) containing unsaturated 3-hydroxydodecenoate unit (approximately 8-9%) when grown with volatile aromatic compounds including benzene, toluene, and ethylbenzene as sole carbon substrate. in particular, when cultivated using a continuous feeding system designed to supply toluene at a flow rate of 0.42gl(-1)h(-1) into a 7-l jar fermentor, the growth of the organism reached up to approximately 3.87gl(-1) af ...201020591653
differential habitat use and niche partitioning by pseudomonas species in human homes.many species of pseudomonas have the ability to use a variety of resources and habitats, and as a result pseudomonas are often characterized as having broad fundamental niches. we questioned whether actual habitat use by pseudomonas species is equally broad. to do this, we sampled extensively to describe the biogeography of pseudomonas within the human home, which presents a wide variety of habitats for microbes that live in close proximity to humans but are not part of the human flora, and for ...201121503776
diversity and phylogeny of bacteria on zimbabwe tobacco leaves estimated by 16s rrna sequence analysis.microorganisms play important roles in the tobacco aging process. however, microbial communities on flue-cured tobacco leaves (fctl) remain largely unknown. in this study, the total microbial genomic dna of unaged and aging fctl from zimbabwe were isolated using a culture-independent method, and the bacterial communities were investigated through analyzing two 16s rrna gene libraries. eighty-four and 65 operational taxonomic units were obtained from the libraries of the unaged and aging fctl, re ...201121660545
assessing the xylanolytic bacterial diversity during the malting process.the presence of microorganisms producing cell wall hydrolyzing enzymes such as xylanases during malting can improve mash filtration behavior and consequently have potential for more efficient wort production. in this study, the xylanolytic bacterial community during malting was assessed by isolation and cultivation on growth media containing arabinoxylan, and identification by 16s rrna gene sequencing. a total of 33 species-level operational taxonomic units (otus) were found, taking into account ...201324010623
isolation and characterization of novel phorate-degrading bacterial species from agricultural soil.based upon 16s rdna sequence homology, 15 phorate-degrading bacteria isolated from sugarcane field soils by selective enrichment were identified to be different species of bacillus, pseudomonas, brevibacterium, and staphylococcus. relative phorate degradation in a mineral salt medium containing phorate (50 μg ml(-1)) as sole carbon source established that all the bacterial species could actively degrade more than 97 % phorate during 21 days. three of these species viz. bacillus aerophilus strain ...201424046230
[antibiotic susceptibility and identification of clinical pseudomonas fulva isolates].the earliest eight clinical strains of pseudomonas fulva were identified in the culture collection of pseudomonads isolated in st. petersburg in 1995-2005, that confirmed the medical importance of the species. a high level of the species identification of all the strains of p. fulva by maldi-tof mass-spectrometry with the use of microflex device with database maldi biotyper (bruker daltonics inc.) was shown. tests for routine studies providing identification of p. fulva without the use of geneti ...201425975114
polymicrobial ventriculitis involving pseudomonas fulva.infections due to pseudomonas fulva remain a rare but emerging concern. a case of ventriculitis due to enterobacter cloacae and pseudomonas fulva following placement of an external ventricular drain is described. similar to other reports, the organism was initially misidentified as pseudomonas putida. the infection was successfully treated with levofloxacin.201424648556
[the identification of clinical strains pseudomonas fulva using techniques of maldi-tof mass spectrometry and common analysis].the technique maldi-tof mass spectrometry was applied using device microflex with database maldi biotyper (bruckeer daltonics inc.) to identify with high level of reliability 8 strains p. fulva from collection of pseudo monads isolated from clinical material in st. petersburg. when analyzing the same strains applying technique maldi tof mass spectrometry using device vitek ms (biomerieux) these starins were wrongly identifies as p. putida. the complex of tests of common analysis was approved and ...201525874304
genome sequence of pseudomonas parafulva crs01-1, an antagonistic bacterium isolated from rice field.pseudomonas parafulva (formerly known as pseudomonas fulva) is an antagonistic bacterium against several rice bacterial and fungal diseases. the total genome size of p. parafulva crs01-1 is 5,087,619 bp with 4389 coding sequences (cdss), 77 trnas, and 7 rrnas. the annotated full genome sequence of the p. parafulva crs01-1 strain might shed light on its role as an antagonistic bacterium.201525835951
microbial diversity observed during hemp retting.historically used in textile and paper industry, hemp fibres have started to find new applications in composite materials with important economic and ecological advantages. however, their applications are limited since manufacturers have some difficulties to standardise fabrication processes. this study is a first step before selection and isolation of strains that could later be used to optimise microbial retting efficiency and hence fibre quality. we studied six samples harvested on different ...201525575888
posttraumatic skin and soft-tissue infection due to pseudomonas fulva.we report a case of posttraumatic skin and soft-tissue infection in a patient with a left thigh wound after a traffic accident. pseudomonas fulva was isolated from a wound aspirate and was identified to the species level by maldi-tof. the patient responded to drainage, debridement of wound, and two weeks of intravenous antibiotic therapy. follow-up after 3 weeks was satisfactory with healthy cover of the injured area.201627752373
high quality draft genome sequences of pseudomonas fulva dsm 17717(t), pseudomonas parafulva dsm 17004(t) and pseudomonas cremoricolorata dsm 17059(t) type strains.pseudomonas has the highest number of species out of any genus of gram-negative bacteria and is phylogenetically divided into several groups. the pseudomonas putida phylogenetic branch includes at least 13 species of environmental and industrial interest, plant-associated bacteria, insect pathogens, and even some members that have been found in clinical specimens. in the context of the genomic encyclopedia of bacteria and archaea project, we present the permanent, high-quality draft genomes of t ...201627594974
unusual microorganisms and antimicrobial resistances in a group of syrian migrants: sentinel surveillance data from an asylum seekers centre in italy.three years of civil war in syria have caused death and increase of communicable diseases. the suffering population has been forced to migrate creating a fertile condition for epidemic spread of infection within the refugee camps.201726987764
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