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characterisation of rotavirus g9 strains isolated in the uk between 1995 and 1998.g9p[6] and g9p[8] rotavirus strains were identified during 1995/96 through the molecular epidemiological surveillance of rotavirus strains circulating in the uk between 1995 and 1998. an increase in the incidence and spread of sporadic infections with rotavirus genotype g9p[8] across the uk was detected in the two following seasons. partial sequencing of the vp7 gene showed that all the uk strains shared a high degree of homology and were related very closely to g9 strains from the us and from s ...200010897071
changing patterns of rotavirus genotypes in ghana: emergence of human rotavirus g9 as a major cause of diarrhea in children.genotyping of human rotaviruses was performed on 312 rotavirus-positive samples collected from 2,205 young children with diarrhea in the upper east district of ghana, a rural community. of the 271 (86.9%) rotavirus strains that could be vp7 (g) or vp4 (p) characterized, 73 (26.9%) were of g9 specificity. the predominant g9 genotype was g9p[8], which constituted 79.5% of all g9 strains detected, followed by g9p[6] (12.3%), g9p[10] (2.7%), and g9p[4] (1.3%). g9 strains with mixed p types constitut ...200312791843
detection of the emerging rotavirus g9 serotype at high frequency in italy.group a human rotavirus strains belonging to the unusual serotype g9 were detected at high frequency in stool specimens from infected children with acute diarrhea in bari, italy, during a 15-month survey from march 2001 to june 2002. this may signify a local reemergence of the g9 rotaviruses detected in italy in the early and mid-1990s or may be related to the global emergence of g9 rotaviruses in recent years.200312904429
an efficient virus concentration method and rt-nested pcr for detection of rotaviruses in environmental water samples.water samples were concentrated by the modified adsorption-elution technique followed by speedvac reconcentration of the filter eluates. reverse transcriptase-nested polymerase chain reaction (rt-nested pcr) was used to detect rotavirus rna in concentrates of the water. the detection limit of the rotavirus determined by rt-nested pcr alone was about 1.67 plaque forming units (pfu) per rt-pcr assay and that by rt-nested pcr combined with concentration from 1l seeded tap water sample was 1.46 plaq ...200415664059
nucleotide variation in the vp7 gene affects pcr genotyping of g9 rotaviruses identified in italy.a modified (aft9m) and a degenerate (aft9d) version of the rotavirus g9-specific primer (aft9) allowed strains that were previously untypable, because of point mutations accumulating at the primer binding site, to be g typed by reverse transcription-polymerase chain reaction. the strains were collected during 2001-2002 in italy in hospitals of the apulia region, from children affected by severe rotavirus-associated enteritis. using a wide selection of g9 rotaviruses detected worldwide, sequencin ...200414635023
predominance of porcine rotavirus g9 in japanese piglets with diarrhea: close relationship of their vp7 genes with those of recent human g9 strains.type g9 of group a rotavirus (gar) was shown to be predominant in a survey of vp7 (g) and vp4 (p) genotypes among porcine gars associated with outbreaks of diarrhea in young pigs in japan between 2000 and 2002. comparison of the g9 vp7 gene sequences showed that the porcine g9 strains were more closely related to human g9 strains reemerging globally since the mid-1990s than to those from the mid-1980s. the vp7 gene sequences of porcine g9 strains from different farms were divergent (6.1 to 7.2% ...200515750112
predominance of rotavirus g9 genotype in children hospitalized for rotavirus gastroenteritis in belgium during 1999-2003.group a rotavirus genotypes g1, g2, g3 and g4 are the main etiological agents of infantile diarrhea. the g9 rotavirus has recently emerged as a fifth important genotype all over the world.200515797358
the emergence of rotavirus genotype g9 in hospitalised children in slovenia.rotavirus g9 genotype was thought to be the fifth most common genotype circulating amongst the population. in previous studies in slovenia, only g1, g3 and g4 genotypes were detected.200515797359
human rotavirus serotype g9, são paulo, brazil, 1996-2003.a total of 3,101 fecal specimens were collected during an 8-year survey for rotavirus infection in são paulo, brazil. group a rotavirus was detected in 774 (25.0%) specimens. of these, 431 strains (55.7%) were analyzed for g and p types by reverse transcription-polymerase chain reaction; g1 was the predominant serotype (68.2%), followed by g9 (17.2%), g4 (6.3%), g2 (1.2%), g3 (0.7%), mixed infection (1.8%), and untypeable (4.6%). both rotavirus g and p types could be established in 332 strains ( ...200616707053
detection of group a human rotavirus g9 genotype circulating in córdoba, argentina, as early as 1980.the incidence of human rotavirus g types was determined over a 25-year period (1979-2003) by using reverse transcription-pcr (rt-pcr) to examine 519 stool specimens found to be positive for rotavirus by enzyme linked immunosorbent assay (elisa) or polyacrylamide gel electrophoresis (page). these stool samples were obtained from children under 3 years old who had been treated for acute diarrhea at public hospitals in córdoba, argentina. the present study describes the continued circulation of the ...200616789026
human rotavirus g9 and g3 as major cause of diarrhea in hospitalized children, spain.in spain, diarrhea remains a major cause of illness among infants and young children. to determine the prevalence of rotavirus genotypes and temporal and geographic differences in strain distribution, a structured surveillance study of hospitalized children <5 years of age with diarrhea was initiated in different regions of spain during 2005. rotavirus was detected alone in samples from 362 (55.2%) samples and as a coinfection with other viruses in 41 samples (6.3%). enteropathogenic bacterial a ...200617176568
genetic heterogeneity, evolution and recombination in emerging g9 rotaviruses.g9 rotavirus is recognized as the emerging genotype spreading around the world. the rapidly increasing detection of this virus in association with the genetic heterogeneity raises questions regarding its origin and epidemiological importance. a total of 380 sequences of rotavirus g9 strains including our sequence data from vietnam and japan, which were detected from 1983 to 2006 in five different continents, were collected from genbank to investigate their heterogeneity and evolution. a novel no ...200717544926
rotavirus g9 severity data revisited. 200717143842
development of a method for concentrating and detecting rotavirus in oysters.identification of enteric viruses in outbreak-implicated bivalve shellfish is difficult because of low levels of contamination and natural inhibitors present in shellfish tissue. in this study, the acid adsorption-alkaline elution method developed in our laboratory was proposed for the detection of rotavirus from oyster samples. the acid adsorption-alkaline elution process included the following steps: acid adsorption at ph 4.8, elution with 2.9% tryptose phosphate broth containing 6% glycine, p ...200818177969
diversity of human rotavirus g9 among children in turkey.between september 2004 and december 2005 a prospective study was conducted to understand the epidemiology of rotavirus infection among children with diarrhea attending two hospitals in ankara, turkey. rotavirus was detected in 39.7% of the 322 stool samples and affected mainly children in the age group of 6-23 months. more than 70% and 39% of these cases occurred in children <2 and <1 year of age, respectively. in the temperate climate of ankara rotavirus infection was prevalent throughout the y ...200818297696
increase in prevalence of human group a rotavirus g9 strains as an important vp7 genotype among children in eastern india.in addition to four globally important group a rotavirus (garv) vp7 genotypes (g1-g4), recent surveillance studies have revealed importance of g9 strains as an aetiological agent of infantile diarrhoea.200818755625
evolutionary consequences of g9 rotaviruses circulating in thailand.infection with group a rotaviruses is the main cause of acute gastroenteritis in infants and young children worldwide. rotavirus g9 is recognized as the fifth most predominant g genotype that spreads throughout the world. in this study, we describe the changing distribution of rotavirus g9 genotype in chiang mai, thailand, from 1989 to 2005. molecular genetic evolutionary analysis of the g9 rotavirus vp7 gene was performed. the g9 rotavirus in thailand was first detected in chiang mai in 1989 wi ...200919716437
[analysis on vp7 and vp4 genes of human rotavirus g9 identified from children with diarrhea in beijing, from 2007 to 2008.].to characterize the outer capsid protein vp7 and vp4 encoding genes of human rotavirus g9 strains detected in beijing, from 2007 to 2008.200920193591
emergence and characterization of human rotavirus g9 strains in tunisia.among human rotaviruses, g9 has emerged as the fifth most important genotype circulating globally. ongoing surveillance of rotavirus in tunisia during the past 10 years identified the first g9 strains in 2004. these strains exhibited the p[8] vp4 genotype and had a long rna electrophoretype. the g9 strains were characterized by phylogenetic analysis of the vp7 gene sequence and showed high identity with other human rotavirus g9 strains belonging to the rotavirus vp7 lineage group iii.200919817604
molecular epidemiology of human astrovirus infections in saudi arabia pediatric patients.earlier work tayeb et al. [tayeb et al. (2008): j med virol 80: 1919-1929] set out to study the epidemiology of diarrhea viruses in pediatric populations. the study addressed initially rotavirus, enteric adenovirus, and astrovirus but was later expanded to include norovirus (nov). viruses were sought in fecal specimens and characterized for genotype using molecular methods (pcr, rt-pcr, and rflp) for the first time in ksa. the survey focused on three locations; jeddah, makkah, and riyadh. during ...201020981791
[analysis for vp4, vp6, nsp4, and nsp5 genes of human rotavirus g9 identified fro 2007-2008, in beijing]. 201022993811
genomic characterization of a cell-culture-adapted korean human g9p[8] rotavirus, cau05-202.the human rotavirus g9 strain is the fifth most common rotavirus worldwide. a human rotavirus g9p[8] strain cau05-202 was isolated from a young child with diarrhea using a cell culture system, and its major gene sequences were determined. phylogenetic analysis of the vp7 gene revealed that cau05-202 clustered into genetic lineage iii-d and was most closely related to g9 rotaviruses from turkey (strain guh13) and sri lanka (strain 05slc056 and 05slc057). vp4 and nsp4 gene analysis showed that cau ...201222274623
molecular characterization of serotype g9 rotaviruses circulating in south korea between 2005 and 2010.a total of 18 rotavirus g9 strains in south korea were collected during five rotavirus seasons between 2005 and 2010. the relationship between these strains was examined by analyzing the genetic variation of two major structural genes, vp7 and vp4. all the rotavirus isolates were of the g9p[8] genotype. the vp7 phylogenetic analysis demonstrated that all of the g9 rotaviruses circulating in south korea belonged to lineage iiid and were within three single clusters. the amino acid comparison of t ...201323154880
milk from brazilian women presents secretory iga antibodies and neutralizes rotavirus g9p[5].to verify the presence of anti-rotavirus serotype g9p[5] siga and the virus neutralization capacity of milk samples from brazilian women.201323850115
clinical and molecular epidemiologic trends reveal the important role of rotavirus in adult infectious gastroenteritis, in shanghai, china.as a leading cause of severe diarrhea in children, the pathogenic role of rotavirus in adults has been underestimated for a long time. a hospital-based prospective clinical and molecular epidemiologic study of rotavirus infections in adults was performed between april 2014 and march 2015 in shanghai, china. overall, rotavirus was detected in 48 of 441 (10.9%) specimens with prevalence peaking in december (33.3%) and january (27.9%), whereas bacteria were identified in 45 of 846 (5.3%) samples (p ...201727884652
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