Publications

carbon dioxide requirement of various species of rumen bacteria.	the carbon dioxide requirement of 32 strains of rumen bacteria, representing 11 different species, was studied in detail. increasing concentrations of co(2) were added as nahco(3) to a specially prepared co(2)-free medium which was tubed and inoculated under nitrogen. prior depletion of co(2) in the inoculum was found to affect the level of requirement; however, the complexity and buffering capacity of the medium did not appear to be involved. an absolute requirement for co(2) was observed for e ...	1971	5541030
effects of heavy metals and other trace elements on the fermentative activity of the rumen microflora and growth of functionally important rumen bacteria.	the inhibitory effects of high concentrations of essential and non-essential trace elements were tested on the rumen microflora using the rate of fermentation in vitro as the assay. the elements (and the concentration causing 50% inhibition) in decreasing order of toxicity were hg2+ (20 microgram/ml), cu2+ (21 microgram/ml), cr6+ (70 microgram/ml), se4+ (73 microgram/ml), ni2+ (160 microgram/ml), cd2+ (175 microgram/ml), as3+ (304 microgram/ml) and as5+ (1610 microgram/ml). the elements tested t ...	1978	565671
the effect of l-alpha-amino-n-butyric acid on growth and production of extracellular isoleucine and valine by eubacterium ruminantium and a related rumen isolate.	two anaerobic rumen bacteria, eubacterium ruminantium and a closely related isolate, were studied to determine the effect of the valine antimetabolite alpha-aminobutyric acid on growth and production of extracellular isoleucine and valine in an amino acid free medium. in the absence of alpha-aminobutyrate, these organisms actively excreted valine during growth (90-195 microgram/ml) but only accumulated limited concentrations of isoleucine (3-7 microgram/ml) in the culture broth. growth of both o ...	1979	575311
stoichiometry of glucose and starch splitting by strains of amylolytic bacteria from the rumen and anaerobic digester.	the stoichiometry of glucose and starch splitting by the amylolytic bacteria streptococcus bovis, selenomonas ruminantium, butyrivibrio fibrisolvens, eubacterium ruminantium and clostridium sp. was followed. there were many differences in the ratios of metabolites and in growth yields, as well as in the cell composition, between the growth on glucose and starch. the bacteria employ different nutritional strategies with respect to both energy sources.	1986	3759723
effects of potassium ion concentrations on the antimicrobial activities of ionophores against ruminal anaerobes.	the antimicrobial activities of monensin and lasalocid against representative strains of ruminal bacteria were evaluated in medium containing three different concentrations of potassium (1.3, 7.9, or 23.3 mm). the growth of eubacterium ruminantium was inhibited by low concentrations of ionophores (less than or equal to 0.16 mg/liter), while the strain of streptococcus bovis tested was resistant to high concentrations of ionophores (40 mg/liter) at all potassium concentrations tested. the mics of ...	1987	3426214
utilization of xylooligosaccharides by selected ruminal bacteria.	the ability of ruminal bacteria to utilize xylooligosaccharides was examined. xylooligosaccharides were prepared by partially hydrolyzing oat spelt xylan in phosphoric acid. this substrate solution was added (0.2%, wt/vol) to a complex medium containing yeast extract and trypticase that was inoculated with individual species of ruminal bacteria, and growth and utilization were monitored over time. all of the xylanolytic bacteria examined were able to utilize this oligosaccharide mixture as a gro ...	1993	8285663
constitutive expression of a heterologous eubacterium ruminantium xylanase gene (xyna) in butyrivibrio fibrisolvens.	an eubacterium ruminantium xylanase gene (xyna) was inserted into pyk4, a shuttle vector replicable in both escherichia coli and butyrivibrio fibrisolvens, and the resultant chimeric plasmid (pyk4xt) was electroporated into b. fibrisolvens ob156c in an attempt to obtain a more xylanolytic b. fibrisolvens. electrotransformants were screened by the development of erythromycin resistance, followed by an activity staining and southern hybridization. the presence of mrna from xyna in the transformant ...	1998	9631539
diet-dependent shifts in the bacterial population of the rumen revealed with real-time pcr.	a set of pcr primers was designed and validated for specific detection and quantification of prevotella ruminicola, prevotella albensis, prevotella bryantii, fibrobacter succinogenes, selenomonas ruminantium-mitsuokella multiacida, streptococcus bovis, ruminococcus flavefaciens, ruminobacter amylophilus, eubacterium ruminantium, treponema bryantii, succinivibrio dextrinosolvens, and anaerovibrio lipolytica. by using these primers and the real-time pcr technique, the corresponding species in the ...	2001	11375193
possible quorum sensing in the rumen microbial community: detection of quorum-sensing signal molecules from rumen bacteria.	the bioluminescence assay using vibrio harveyi bb170 was used to examine quorum-sensing autoinducer 2 (ai-2) activity from cell-free culture fluids of rumen bacteria. the assay showed that the culture fluids of four species of rumen bacteria, butyrivibrio fibrisolvens, eubacterium ruminantium, ruminococcus flavefaciens, and succinimonas amylolytica, contained ai-2-like molecules. furthermore, homologues for luxs genes were detected in rumen fluids collected from three cows and in bacterial cells ...	2003	12594022
in vitro bacterial growth and in vivo ruminal microbiota populations associated with bloat in steers grazing wheat forage.	the role of ruminal bacteria in the frothy bloat complex common to cattle grazing winter wheat has not been previously determined. two experiments, one in vitro and another in vivo, were designed to elucidate the effects of fresh wheat forage on bacterial growth, biofilm complexes, rumen fermentation end products, rumen bacterial diversity, and bloat potential. in exp. 1, 6 strains of ruminal bacteria (streptococcus bovis strain 26, prevotella ruminicola strain 23, eubacterium ruminantium b1c23, ...	2006	16971591
metabolism of polyunsaturated fatty acids and their toxicity to the microflora of the rumen.	ruminal microorganisms hydrogenate polyunsaturated fatty acids (pufa) present in forages and thereby restrict the availability of health-promoting pufa in meat and milk. the aim of this study was to investigate pufa metabolism and the influence of pufa on members of the ruminal microflora. eleven of 26 predominant species of ruminal bacteria metabolised linoleic acid (la; cis-9,cis-12-18:2) substantially. the most common product was vaccenic acid (trans-11-18:1), produced by species related to b ...	2007	17072533
dominance of prevotella and low abundance of classical ruminal bacterial species in the bovine rumen revealed by relative quantification real-time pcr.	relative quantification real-time pcr was used to quantify several bacterial species in ruminal samples from two lactating cows, each sampled 3 h after feeding on two successive days. abundance of each target taxon was calculated as a fraction of the total 16s rrna gene copies in the samples, using taxon-specific and eubacterial domain-level primers. bacterial populations showed a clear predominance of members of the genus prevotella, which comprised 42% to 60% of the bacterial rrna gene copies ...	2007	17235560
effect of monensin feeding and withdrawal on populations of individual bacterial species in the rumen of lactating dairy cows fed high-starch rations.	real-time polymerase chain reaction (pcr) was used to quantify 16 procaryotic taxa in the rumina of two lactating dairy cows following supply and subsequent withdrawal of the feed additive monensin (13.9 mg/kg of diet dry matter) in a high-starch, silage-based ration. pcr was conducted on dna from rumen samples collected 6 h post feeding on two successive days before monensin supplementation, after 30 days of monensin supplementation, and at six weekly intervals after monensin withdrawal. mean v ...	2008	18535825
biotransformation of 2,4,6-trinitrotoluene by pure culture ruminal bacteria.	twenty-one ruminal bacteria species were tested for their ability to degrade 2,4,6-trinitrotoluene (tnt) within 24 h. butyrivibrio fibrisolvens, fibrobacter succinogenes, lactobacillus vitulinus, selenomonas ruminantium, streptococcus caprinus, and succinivibrio dextrinosolvens were able to completely degrade 100 mg/l tnt, with <5% of the original tnt recovered as diaminonitrotoluene metabolites. eubacterium ruminantium, lactobacillus ruminis, ruminobacter amylophilus, streptococcus bovis, and w ...	2009	18839246
molecular diversity of bacteria in yunnan yellow cattle (bos taurs) from nujiang region, china.	the rumen content of four yunnan yellow cattle (bos taurs) were collected to determine the bacteria diversity by using 16s rrna gene sequence analysis. a total of 129 sequences were examined and the sequences were referred as 107 otu (operational taxonomy unit) according to the similarity level of 97% in gene sequence. similarity analysis revealed that yunnan yellow cattle had 12 sequences (10 otu) shared 97% or greater similarity with cultured rumen bacteria butyrivibrio fibrisolvens, succinicl ...	2011	21598111
[diversity of soil bacterial community in banana orchards infected with wilt disease].	six soil samples including 3 wilt disease-infected samples and 3 disease-free samples were collected from the banana orchards in 3 areas in lingao county, hainan province of south china. the soil physical and chemical properties were determined by conventional methods, and the diversity of soil bacterial community was analyzed by terminal restriction fragment length polymorphism (t-rflp). then, the relationships between the soil bacterial community composition and the soil physical and chemical ...	2013	24380349
the core faecal bacterial microbiome of irish thoroughbred racehorses.	in this study, we characterized the gut microbiota in six healthy irish thoroughbred racehorses and showed it to be dominated by the phyla firmicutes, bacteroidetes, proteobacteria, verrucomicrobia, actinobacteria, euryarchaeota, fibrobacteres and spirochaetes. moreover, all the horses harboured clostridium, fibrobacter, faecalibacterium, ruminococcus, eubacterium, oscillospira, blautia anaerotruncus, coprococcus, treponema and lactobacillus spp. notwithstanding the sample size, it was noteworth ...	2013	23889584
analysis of rumen microbial populations in lactating dairy cattle fed diets varying in carbohydrate profiles and saccharomyces cerevisiae fermentation product.	the rumen microbial ecosystem is a critical factor that links diets to bovine physiology and productivity; however, information about dietary effects on microbial populations has generally been limited to small numbers of samples and qualitative assessment. to assess whether consistent shifts in microbial populations occur in response to common dietary manipulations in dairy cattle, samples of rumen contents were collected from 2 studies for analysis by quantitative real-time pcr (qpcr). in one ...	2013	23849646
potentiation by metal ions of the efficacy of the ionophores, monensin and tetronasin, towards four species of ruminal bacteria.	concentrations of na(+), k(+) and ca(2+) in the growth medium were varied within limits normally found in vivo to determine how cation concentrations affect the sensitivity of ruminal bacteria to the ionophores, monensin (a na(+)/h(+) and k(+)/h(+) exchanger) and tetronasin (ca(2+)/h(+)). high [na(+)] (172 mm cf. 137 mm in control medium) enhanced the efficacy of monensin towards eubacterium ruminantium 2388, streptococcus bovis c277, lactobacillus casei lb17 and prevotella albensis m384. high [ ...	2013	23210858