Publications

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fungi from otitis media of agricultural field workers.twenty four fungi were isolated from ear wax or otitis media of agricultural field workers, of which 18 are being reported for the first time from india. direct observation, revealed the presence of hyphal fragments, spores of alternaria, bipolaris, conidial head of aspergillus, smut teliospores and pollen. spores of only four fungi, alternaria humicola, aspergillus niveus, bipolaris spicifera and fusarium moniliforme germinated in otitis media at 37 degrees c, and their germination was affected ...19846527698
rapid and sensitive detection of citrinin production during fungal fermentation using high-performance liquid chromatography.a rapid and sensitive assay was developed for the detection of the mycotoxin citrinin by reversed-phase chromatography. citrinin was eluted from a radical-compression c18 column with a retention time of 3.86 min (flow-rate of 2.5 ml/min) with acetonitrile-water-acetic acid (40:59:1) containing tetrabutylammonium phosphate (0.0025 m) [corrected]. comparative analysis revealed fluorescence detection to be 100 times more sensitive than detection by conventional ultraviolet absorbance. the fluoresce ...19902089062
separation and partial purification of beta-glucosidase and two endoglucanases in aspergillus niveus.the thermotolerant aspergillus niveus strain rmf 7883 was grown in czapek medium, with filter paper cellulose. the proportion of mycelial-bound to extracellular enzymes was studied. most of the beta-glucosidase (80.9%) and endoglucanases (78.3%) activities were extracellular. the extracellular endoglucanases and beta-glucosidase were separated and partially purified by sephadex g-100 gel filtration, followed by ion exchange chromatography on cm-trisacryl m. two extracellular endoglucanases, eg i ...19969019140
biotreatment of distillery effluent using aspergillus niveus. 200312545358
aspochalamins a-d and aspochalasin z produced by the endosymbiotic fungus aspergillus niveus lu 9575. i. taxonomy, fermentation, isolation and biological activities.aspochalamins a-d, a family of new cytochalasan antibiotics have been isolated from aspergillus niveus, an endosymbiotic fungus isolated from the gut of a woodlouse belonging to the family trichoniscidae. besides aspochalamins, aspochalasin z, a new member of the aspochalasin family, as well as the known mycotoxins aspochalasin d and citreoviridins a/c and b were isolated from the mycelium. aspochalamins showed cytostatic effects towards various tumor cell lines and a weak antibacterial activity ...200415712664
aspochalamins a-d and aspochalasin z produced by the endosymbiotic fungus aspergillus niveus lu 9575. ii. structure elucidation.the structures of new cytochalasan fungal metabolites aspochalamins a-d have been elucidated by esi-fticr-ms, nmr spectroscopy, and chiral amino acid analysis. aspochalamins a-d consist of different aspochalasin skeletons connected at position c-19 to the n terminus of the tripeptidic moiety amide anthranoyl-l-alanine-e-didehydrotryptamide. furthermore, the structure of a new aspochalasin analog, aspochalasin z, was derived from its molecular mass and nmr data as 10-isopropyl-14-methyl[11]-cytoc ...200415712665
evolutionary relationships among aspergillus terreus isolates and their relatives.aspergillus terreus is a ubiquitous fungus in our environment. it is an opportunistic human pathogen and economically important as the main producer of lovastatin, a cholesterol lowering drug. our aim was to examine the genetic variability of a. terreus and closely related species using molecular and analytical techniques. lovastatin production was examined by hplc. lovastatin was produced by seven isolates belonging to the species a. terreus. rapd analyses were carried out using 25 different ra ...200516096690
first case of breakthrough pulmonary aspergillus niveus infection in a patient after allogeneic hematopoietic stem cell transplantation.aspergillus niveus is a species uncommon in clinical samples, and to date, invasive fungal infections caused by this fungal pathogen have not been described. this is the 1st report on a pulmonary breakthrough aspergillosis caused by a. niveus in a 21-year-old woman after allogeneic hematopoietic stem cell transplantation for fanconi anemia.200818687556
production of xylanase by aspergilli using alternative carbon sources: application of the crude extract on cellulose pulp biobleaching.the ability of xylanolytic enzymes produced by aspergillus fumigatus rp04 and aspergillus niveus rp05 to promote the biobleaching of cellulose pulp was investigated. both fungi grew for 4-5 days in liquid medium at 40 degrees c, under static conditions. xylanase production was tested using different carbon sources, including some types of xylans. a. fumigatus produced high levels of xylanase on agricultural residues (corncob or wheat bran), whereas a. niveus produced more xylanase on birchwood x ...200918923855
enhanced in vitro and in vivo antioxidant activity and mobilization of free phenolic compounds of soybean flour fermented with different beta-glucosidase-producing fungi.to evaluate the soybean polyphenol glucosides bioconversion to aglycone forms by different beta-glucosidases-producing filamentous fungi to enhance their antioxidant activity.200919200313
xylanases from aspergillus niger, aspergillus niveus and aspergillus ochraceus produced under solid-state fermentation and their application in cellulose pulp bleaching.this study describes the production of xylanases from aspergillus niveus, a. niger, and a. ochraceus under solid-state fermentation using agro-industrial residues as substrates. enzyme production was improved using a mixture of wheat bran and yeast extract or peptone. when a mixture of corncob and wheat bran was used, xylanase production from a. niger and a. ochraceus increased by 18%. all cultures were incubated at 30 degrees c at 70-80% relative humidity for 96 h. for biobleaching assays, 10 o ...200919271244
properties of a purified thermostable glucoamylase from aspergillus niveus.a glucoamylase from aspergillus niveus was produced by submerged fermentation in khanna medium, initial ph 6.5 for 72 h, at 40 degrees c. the enzyme was purified by deae-fractogel and concanavalin a-sepharose chromatography. the enzyme showed 11% carbohydrate content, an isoelectric point of 3.8 and a molecular mass of 77 and 76 kda estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis or bio-sil-sec-400 gel filtration, respectively. the ph optimum was 5.0-5.5, and the enzyme re ...200919697071
purification and biochemical characterization of a novel alpha-glucosidase from aspergillus niveus.an extracellular a-glucosidase produced by aspergillus niveus was purified using deae-fractogel ion-exchange chromatography and sephacryl s-200 gel filtration. the purified protein migrated as a single band in 5% page and 10% sds-page. the enzyme presented 29% of glycosylation, an isoelectric point of 6.8 and a molecular weight of 56 and 52 kda as estimated by sds-page and bio-sil-sec-400 gel filtration column, respectively. the enzyme showed typical alpha-glucosidase activity, hydrolyzing p-nit ...200919757138
tunicamycin inhibition of n-glycosylation of α-glucosidase from aspergillus niveus: partial influence on biochemical properties.treatment of aspergillus niveus with 30 μg tunicamycin/ml did not interfere with α-glucosidase production, secretion, or its catalytic properties. fully- and under-glycosylated forms of the enzyme had similar molecular masses, ~56 kda. moreover, the absence of n-glycans did not affect either ph optimum (6.0) or temperature optimum (65°c). the k(m) and v(max) values of under- and fully-glycosylated forms of α-glucosidase were similar when assessed for hydrolysis of starch (~0.6 mg/ml, ~350 μmol g ...201020490616
biotechnological potential of agro-industrial wastes as a carbon source to thermostable polygalacturonase production in aspergillus niveus.agro-industrial wastes are mainly composed of complex polysaccharides that might serve as nutrients for microbial growth and production of enzymes. the aim of this work was to study polygalacturonase (pg) production by aspergillus niveus cultured on liquid or solid media supplemented with agro-industrial wastes. submerged fermentation (sbmf) was tested using czapeck media supplemented with 28 different carbon sources. among these, orange peel was the best pg inducer. on the other hand, for solid ...201121837272
functional characterization and oligomerization of a recombinant xyloglucan-specific endo-β-1,4-glucanase (gh12) from aspergillus niveus.xyloglucan is a major structural polysaccharide of the primary (growing) cell wall of higher plants. it consists of a cellulosic backbone (beta-1,4-linked glucosyl residues) that is frequently substituted with side chains. this report describes aspergillus nidulans strain a773 recombinant secretion of a dimeric xyloglucan-specific endo-β-1,4-glucanohydrolase (xega) cloned from aspergillus niveus. the orf of the a. niveus xega gene is comprised of 714 nucleotides, and encodes a 238 amino acid pro ...201122230786
New species in Aspergillus section Terrei.Section Terrei of Aspergillus was studied using a polyphasic approach including sequence analysis of parts of the ß-tubulin and calmodulin genes and the ITS region, macro- and micromorphological analyses and examination of extrolite profiles to describe three new species in this section. Based on phylogenetic analysis of calmodulin and ß-tubulin sequences seven lineages were observed among isolates that have previously been treated as A. terreus and its subspecies by Raper & Fennell (1965) and o ...201121892242
functional properties of a manganese-activated exo-polygalacturonase produced by a thermotolerant fungus aspergillus niveus.a thermotolerant fungus identified as aspergillus niveus was isolated from decomposing materials and it has produced excellent levels of hydrolytic enzymes that degrade plant cell walls. a. niveus germinated faster at 40 °c, presenting protein levels almost twofold higher than at 25 °c. the crude extract of the a. niveus culture was purified by diethylaminoethyl (deae)-cellulose, followed by biogel p-100 column. polygalacturonase (pg) is a glycoprotein with 37.7 % carbohydrate, molecular mass of ...201323613286
antioxidant activity and total phenolic content of endophytic fungus fennellia nivea nrrl 5504.antioxidant is an interesting bioactivity since it has several correlations with disease such as alzheimer, cancer, ageing and many others in which were promoted by free radicals. investigation on our endophytic fungus collection namely fennelia nivea nrrl 5504 revealed that the fermented mediumhas antioxidant property against dpph through free radical scavenging effect. fermentation in liquid medium i.e. potatoes dextrose broth, resulted that the fermented medium reached about 86.51% (p < 0.05) ...201324511703
purification, partial characterization, and covalent immobilization-stabilization of an extracellular α-amylase from aspergillus niveus.an extracellular amylase secreted by aspergillus niveus was purified using deae fractogel ion exchange chromatography and sephacryl s-200 gel filtration. the purified protein migrated as a single band in 5 % polyacrylamide gel electrophoresis (page) and 10 % sodium dodecyl sulfate (sds-page). the enzyme exhibited 4.5 % carbohydrate content, 6.6 isoelectric point, and 60 and 52 kda molar mass estimated by sds-page and bio-sil-sec-400 gel filtration column, respectively. the amylase efficiently hy ...201323463193
[antibiotic activity of some fungi].biological activity of pure extracts of cultural filtrates of aspergillus niveus 2411, myrothecium cinctum 910, ulocladium consortiale 960, penicillium sp. 10-51 concerning wide spectrum of test-organisms was investigated. it was shown that the extracts had high levels of antibacterial activity against gram-positive microorganisms, especially against bacillus genus. but their activity against gram-negative bacteria was a bit lower. on the other hand, metabolites of m. cinctum 910 and penicillium ...201424479314
enhanced xyloglucan-specific endo-β-1,4-glucanase efficiency in an engineered cbm44-xega chimera.xyloglucan-specific endo-β-1,4-glucanases (xegs, ec 3.2.1.151) exhibit high catalytic specificity for β-1,4 linkages of xyloglucan, a branched hemicellulosic polysaccharide abundant in dicot primary cell walls and present in many monocot species. in nature, gh12 xegs are not associated with carbohydrate-binding modules (cbms), and here, we have investigated the effect of the fusion of the xyloglucan-specific cbm44 on the structure and function of a gh12 xeg from aspergillus niveus (xega). this f ...201525605422
[herbicide activity of some micromycetes].screening of phytotoxic properties of 5 micromycete strains were made concerning cultivated plants and weeds. all of the strains show phytotoxic activity of different levels. in particular, the cultural filtrates of penicillium sp. 10-51 and aspergillus niveus 2411 suppressed the growth of seeds of galinsoga parviflora, amaranthus retroflexus and capsella bursa pastoris. the cultural filtrate of ulocladium consortiale 960 suppressed the growth of panicum capillare and setaria viridis. the greate ...201522830196
optimization of chromium and tannic acid bioremediation by aspergillus niveus using plackett-burman design and response surface methodology.a chromium and tannic acid resistance fungal strain was isolated from tannery effluent, and identified as aspergillus niveus mcc 1318 based on its rdna gene sequence. the mic (minimum inhibitory concentration) of the isolate against chromium and tannic acid was found to be 200 ppm and 5% respectively. optimization of physiochemical parameters for biosorption of chromium and tannic acid degradation was carried out by plackett-burman design followed by response surface methodology (rsm). the maxim ...201729138995
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