Publications

tissue reaction to glugea plecoglossi infection in its natural host, plecoglussus altivelis.	the microsporidian glugea plecoglossi induces in its fish host plecoglossus altivelis a hypertrophic growth of the infected cells, resulting in formation of large xenomas. early thin-walled xenomas did not elicit any reaction of the surrounding tissue. later, however, coinciding with the mature xenoma being filled up with a growing mass of spores, proliferative inflammatory reaction sets in, changing gradually the xenoma into a granuloma. the granulation tissue grows into the spore mass. the spo ...	1980	6774933
protein phylogeny of translation elongation factor ef-1 alpha suggests microsporidians are extremely ancient eukaryotes.	partial regions of the mrna encoding a major part of translation elongation factor 1 alpha (ef-1 alpha) from a mitochondrion-lacking protozoan, glugea plecoglossi, that belongs to microsporidians, were amplified by polymerase chain reaction (pcr) and their primary structures were analyzed. the deduced amino acid sequence was highly divergent from typical ef-1 alpha's of eukaryotes, although it clearly showed a eukaryotic feature when aligned with homologs of the three primary kingdoms. maximum l ...	1996	8919877
complete nucleotide sequences of the genes encoding translation elongation factors 1 alpha and 2 from a microsporidian parasite, glugea plecoglossi: implications for the deepest branching of eukaryotes.	complete nucleotide sequences of the genes putatively encoding translation elongation factors 1 alpha (ef-1 alpha) and 2 (ef-2) from a mitochondrion-lacking protozoan, glugea plecoglossi, that belongs to microsporidians were determined. the deduced amino acid sequences of the putative ef-1 alpha and ef-2 of gl. plecoglossi showed very unusual features compared with typical eukaryotic sequences. the degree of divergence was especially great in the ef-1 alpha sequence, although it clearly showed a ...	1996	9010756
phylogenetic position of the mitochondrion-lacking protozoan trichomonas tenax, based on amino acid sequences of elongation factors 1alpha and 2.	major parts of amino-acid-coding regions of elongation factor (ef)-1alpha and ef-2 in trichomonas tenax were amplified by pcr from total genomic dna and the products were cloned into a plasmid vector, pgem-t. the three clones from each of the products of the ef-1alpha and ef-2 were isolated and sequenced. the insert dnas of the clones containing ef-1alpha coding regions were each 1,185 bp long with the same nucleotide sequence and contained 53.1% of g + c nucleotides. those of the clones contain ...	1997	9010141
respiratory burst assay of head kidney macrophages of ayu, plecoglossus altivelis, stimulated with glugea plecoglossi (protozoa: microspora) spores.	the respiratory burst assay was conducted using the nitroblue tetrazolium reduction method and horseradish peroxidase method to investigate the events when ayu head kidney macrophages phagocytize fresh and formalin-killed glugea plecoglossi spores. the production of o2 against g. plecoglossi spores was negligible compared to zymosan (p < 0.01). zymosan-induced o2 production was markedly inhibited by adding g. plecoglossi spores simultaneously (p < 0.01). this phenomenon was dose dependent, and k ...	1998	9645856
lectin-reactive components of the microsporidian glugea plecoglossi and their relation to spore phagocytosis by head kidney macrophages of ayu plecoglossus altivelis.	we investigated the reactivity of lectins to spores of glugea plecoglossi from ayu plecoglossus altivelis. smear preparations of purified spores were treated with 8 kinds of lectins. lectin blots were used to detect glycoproteins of spore lysates. in addition, lectin-treated spores were applied to head kidney macrophages of ayu, and the percentage of phagocytosis (pp) was calculated and compared with the control. two lectins (cona, wga) reacted with the surface of the spores, and a major band (5 ...	1999	11407405
mitochondrial-type hsp70 genes of the amitochondriate protists, giardia intestinalis, entamoeba histolytica and two microsporidians.	genes encoding putative mitochondrial-type heat shock protein 70 (mit-hsp70) were isolated and sequenced from amitochondriate protists, giardia intestinalis, entamoeba histolytica, and two microsporidians, encephalitozoon hellem and glugea plecoglossi. the deduced mit-hsp70 sequences were analyzed by sequence alignments and phylogenetic reconstructions. the mit-hsp70 sequence of these four amitochondriate protists were divergent from other mit-hsp70 sequences of mitochondriate eukaryotes. howeve ...	2002	11880223
modes of transmission of glugea plecoglossi (microspora) via the skin and digestive tract in an experimental infection model using rainbow trout, oncorhynchus mykiss (walbaum).	glugea plecoglossi (microspora) is a significant cause of economic loss in cultured ayu, plecoglossus altivelis, in japan, due to the unsightly appearance of infected fish harbouring xenomas in the body cavity. modes of transmission of g. plecoglossi via the skin and digestive tract were studied in an experimental infection model using rainbow trout, oncorhynchus mykiss. combined with uvitex 2b and in situ hybridization (ish) assays, the early development of g. plecoglossi was successfully trace ...	2004	15291785
isolation and characterization of l-rhamnose-binding lectin, which binds to microsporidian glugea plecoglossi, from ayu (plecoglossus altivelis) eggs.	a rhamnose-binding lectin, named sfl, was isolated from the eggs of ayu (sweet fish, plecoglossus altivelis) by affinity and ion-exchange chromatographies. sfl revealed 287 amino acid residues with 3 tandemly repeated domains, and contained 8 half-cys residues in each domain. the lectin was shown to have a highly specific binding affinity to globotriaosylceramide (gb3) by frontal affinity chromatography using 100 oligosaccharides. sfl was localized in several tissues and serum of both male and f ...	2008	17997156
first record of glugea plecoglossi (takahashi & egusa, 1977), a microsporidian parasite of ayu (plecoglossus altivelis altivelis temminck & schlegel, 1846) in mainland china.		2017	28707704