Publications

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serum hormone patterns during abortion in the bolivian squirrel monkey.serum measurements of chorionic gonadotropin (cg), estradiol (e-2) and progesterone (p) were used to describe patterns of hormonal change in bolivian squirrel monkeys undergoing spontaneous abortion. during early pregnancy, serum cg levels gradually increased, reaching maximum levels at the end of the first 50 days of pregnancy (range: 200-1964 ug protein/ml). e-2 concentrations also increased to high levels (10-30 ng/ml) toward the end of pregnancy, while serum p remained fairly constant at lev ...19854094399
tritrichomonas mobilensis n. sp. (zoomastigophorea: trichomonadida) from the bolivian squirrel monkey saimiri boliviensis boliviensis.a trichomonad flagellate, tritrichomonas mobilensis n. sp., is described from the large intestine of the squirrel monkey, saimiri boliviensis boliviensis. the organism has a lanceolate body 7-10.5 micrometers in length; a well developed undulating membrane; a stout, tubular axostyle with periaxostylar rings that terminate in a cone-shaped segment projecting from the posterior end of the cell; and a moderately wide costa. the anterior flagella are about as long as the body, and the recurrent flag ...19863735158
pathologic changes associated with fatal plasmodium falciparum infection in the bolivian squirrel monkey (saimiri sciureus boliviensis).fatal cases of experimental plasmodium falciparum (indochina i) in bolivian squirrel monkeys (saimiri sciureus boliviensis) were examined by histologic and ultrastructural methods. gross lesions were characterized by hepatosplenomegaly and interstitial pulmonary changes. histologically, there was marked diffuse reticuloendothelial hyperplasia, pulmonary alveolar septal thickening, mesangioproliferative glomerulonephropathy, sequestration of parasitized erythrocytes in deep vascular beds, degener ...19873300388
serum oestradiol, progesterone, chorionic gonadotrophin and prolactin concentrations during pregnancy in the bolivian squirrel monkey (saimiri sciureus).concentrations of chorionic gonadotrophin gradually increased during early pregnancy, reached maximum values at mid-gestation, then declined to low levels. oestradiol-17 beta concentrations were relatively low (300-600 pg/ml) during early pregnancy, increased thereafter to high levels (10-35 ng/ml), and then appeared to decline towards term. concentrations of progesterone were constant (100-200 ng/ml) for the first 15 weeks of gestation, suggesting that sequential measurements of progesterone co ...19873656275
mating-related estradiol fluctuations during the estrous cycle of the bolivian squirrel monkey (saimiri boliviensis boliviensis).the female squirrel monkey, saimiri boliviensis, a new world monkey, has 10-day estrous cycles during the annual breeding season. measurements of serum estradiol (e) concentrations in females housed with males in breeding pens revealed markedly higher levels than previously reported. additionally, females in breeding pens appeared to have two distinct patterns of serum e peaks relative to the lh surge. serum estrogen peaks averaging 5-fold greater than levels on the preceding day were observed o ...19912043735
fasting hyperbilirubinemia in normal squirrel monkeys.the plasma of bolivian squirrel monkeys, unlike that of brazilian squirrel monkeys, is markedly yellow due to unconjugated hyperbilirubinemia after an overnight fast. the fasting hyperbilirubinemia in bolivian squirrel monkeys is likely due to two mechanisms. first, a twofold increase in the bilirubin turnover/production rate occurs during a 24-hour fast. a second mechanism is the decreased hepatic conjugation potential for bilirubin due to the presence of a higher bilirubin udp-glucuronosyltran ...19921316506
equine fasting hyperbilirubinemia.it appears that different mechanisms responsible for fasting hyperbilirubinemia may be operative in different mammalian species (and subspecies). an increase in bilirubin production does not seem to occur in the horse, but a decrease in the hepatic uptake of bilirubin has been supported by a number of studies. even though the delay in plasma elimination could also result from a decrease in hepatic blood flow, this possibility does not seem to play a major role since the hepatic uptake of compoun ...19938273511
restoration of oocyte maturational competency during the nonbreeding season with follicle-stimulating hormone stimulation in squirrel monkeys (saimiri boliviensis boliviensis).the in vitro maturation potential of oocytes retrieved during the nonbreeding season with or without prior in vivo low-dose fsh stimulation was studied in adult squirrel monkeys (saimiri boliviensis boliviensis). additionally, the adequacy of various protein supplements in media used for oocyte maturation was investigated. ovaries were removed from animals in the nonbreeding season (n = 9) with or without prior treatment with a low dose (1 mg) of fsh for four days before ovariectomy. minimal est ...19948142548
strategy using pooled dna to identify 56 short tandem repeat polymorphisms for the bolivian squirrel monkey. 19968891203
cloning and expression of the glucocorticoid receptor from the squirrel monkey (saimiri boliviensis boliviensis), a glucocorticoid-resistant primate.new world primates such as the squirrel monkey have elevated cortisol levels and glucocorticoid resistance. we have shown that the apparent binding affinity of the glucocorticoid receptor in squirrel monkey lymphocytes is 5-fold lower than that in human lymphocytes (apparent kd, 20.9 +/- 1.8 and 4.3 +/- 0.2 nmol/l, respectively; n = 3), consistent with previous studies in mononuclear leukocytes isolated from the two species. as a first step in understanding the mechanism of decreased binding aff ...19979024238
glucocorticoid-resistant b-lymphoblast cell line derived from the bolivian squirrel monkey (saimiri boliviensis boliviensis).the goal of the study reported here was to develop a continuous cell line from the squirrel monkey that expresses the species-specific phenotype of impaired sensitivity to glucocorticoids. thirty milliliters of blood from a male bolivian squirrel monkey (saimiri boliviensis boliviensis) was fractionated, and the buffy coat was obtained and incubated in the presence of b95-8 cell-conditioned medium, an abundant source of epstein-barr virus (ebv), and 2 micrograms of cyclosporin a/ml. cell growth ...199810090044
determination of fetal biparietal diameter without the use of ultrasound in squirrel monkeys.this study evaluated manual caliper measurement of fetal bpd in saimiri through the abdomen of the dam (tbpd) for correlation with paired ultrasound measurements, prediction of delivery date, subspecies variation, prediction of pregnancy outcome, and correlation between postpartum bpd and tbpd. regression analysis revealed a close relationship between tbpd measurements and those obtained by ultrasound (p < 0.001). tbpd for saimiri boliviensis boliviensis increased from a mean of 14.8 +/- 0.3 mm ...19989926983
presence of platelet-activating factor in rhesus (macaca mulatta) spermatozoa.platelet-activating factor [1-o-alkyl-2-acetyl-sn-glycero-phosphocholine; paf] is a unique signaling phospholipid which has been implicated in a number of biological activities (e.g., reproduction). paf has been detected in the spermatozoa from a number of laboratory and domestic species, including, but not limited to, rabbit, bovine, and the mouse. the concentration of paf is inversely related to human (homo sapien) spermatozoal quality. additionally, paf levels are significantly higher in boli ...199910372538
impaired transactivation of the glucocorticoid receptor cloned from the guyanese squirrel monkey.squirrel monkeys are among a diverse group of new world primates that demonstrate unusually high levels of circulating corticosteroids and glucocorticoid receptor (gr) insensitivity. recent evidence suggests that overexpression of an immunophilin impairs dexamethasone binding to gr in the bolivian squirrel monkey (saimiri boliviensis). here we describe the cloning, expression, and functional characterization of gr from the closely related guyanese squirrel monkey (s. sciureus). the cloned guyane ...200010775802
the origin of four squirrel monkey cell lines established by karyotype analysis.the squirrel monkey is a neotropical primate genus which is widely used in biomedical research but includes individual species and subspecies that respond differently to experimental perturbations. gtg-banding patterns of chromosomes 15 and 16, which are distinct among different squirrel monkey species and subspecies, were used to determine the origin of three lung fibroblast cell lines from squirrel monkeys of unknown genetic background (dpso 114/74, sqmklu/68, and 7603830) and to confirm the o ...200111528123
overexpression of the fk506-binding immunophilin fkbp51 is the common cause of glucocorticoid resistance in three new world primates.many new world primates have high circulating levels of cortisol to compensate for the expression of glucocorticoid receptors (grs) with low activity. recent work in squirrel monkeys has suggested that this may be due to either the expression of grs that are transcriptionally incompetent or the expression of an fk506-binding immunophilin that inhibits gr binding. the goal of this study was to resolve this controversy by determining the molecular basis of glucocorticoid resistance not only in spe ...200111703081
a kidney epithelial cell line from a bolivian squirrel monkey.squirrel monkeys are the most commonly used new world primates in biomedical research, but in vitro studies are restricted by the limited number of cell lines available from this species. we report here the development and characterization of a continuous, kidney epithelial cell line (sqmk-fp cells) derived from a newborn squirrel monkey. karyotype was consistent with bolivian squirrel monkey (submetacentric chromosome pair 15 and acrocentric chromosome pair 16). all cells examined were hyperdip ...200212418921
plasmodium simium and saimiri boliviensis as a model system for testing candidate vaccines against plasmodium vivax.observations on plasmodium simium infections in saimiri boliviensis boliviensis monkeys suggest that this host-parasite combination would be a suitable model for the testing of candidate vaccines against plasmodium vivax. to evaluate the normal course of infections, parasitemia in 52 splenectomized s. boliviensis boliviensis monkeys infected with p. simium were analyzed. the mean maximum parasite count for 31 monkeys after injection with trophozoite-infected erythrocytes was 77,580/microl. twent ...200516172496
age-related diffuse chronic telogen effluvium- type alopecia in female squirrel monkeys (saimiri boliviensis boliviensis).we investigated the diffuse alopecia affecting some female squirrel monkeys (saimiri boliviensis boliviensis) housed in a breeding facility. we randomly selected 100 female and 10 male animals and performed a complete physical exam and a hair assessment on all animals; blood tests, trichograms, hair density; and skin biopsies in representative cases; and a dominance behavioral assessment of 50 animals. hair coat was normal in 35 female monkeys and all 10 male animals. of the 65 females with diff ...200515884780
cortisol metabolism in the bolivian squirrel monkey (saimiri boliviensis boliviensis).new world squirrel monkeys (saimiri spp.) have high circulating cortisol levels but normal electrolytes and blood pressures. the goal of the present study was to gain insight into adaptive mechanisms used by bolivian squirrel monkeys to minimize the effects of high cortisol on mineralocorticoid receptor (mr) activity and electrolyte and water balance. aldosterone levels in serum from 10 squirrel monkeys were 17.7 +/- 3.4 ng/dl (normal range in humans, 4 to 31 ng/dl), suggesting that squirrel mon ...200616639980
ovarian stimulation of squirrel monkeys (saimiri boliviensis boliviensis) using pregnant mare serum gonadotropin.the application of assisted reproductive technologies (art) to nonhuman primates has created opportunities for improving reproductive management in breeding colonies, and for creation of new animal models by genetic modification. one impediment to the application of art in saimiri spp. has been the lack of an effective gonadotropin preparation for ovarian stimulation. pregnant mare serum gonadotropin (pmsg) is inexpensive and readily available, but its repeated use in rhesus monkeys has been ass ...200616521854
meiotic characterization and sex determination system of neotropical primates: bolivian squirrel monkey saimiri boliviensis (primates: cebidae).the chromosomal sex determination system differs among platyrrhine monkeys more than any other group of primates. although a number of studies have investigated mitotic chromosomes across platyrrhine species, the meiotic chromosomes of many genera have not yet been described. the goal of this study was to characterize the sex determination system of saimiri boliviensis. we described for the first time the meiotic cycle, confirming the sexual system in germ cells from testicular biopsies of four ...200717387714
the composition of milk from bolivian squirrel monkeys (saimiri boliviensis boliviensis).squirrel monkeys (genus saimiri) give birth to relatively large neonates with large, fast-growing brains. maternal energy expenditure during gestation and infant development is argued to be high, but may be offset by the provisioning of offspring by females other than the mother (allonursing). milk composition is an important component of maternal energy expenditure, but has been examined in only a small number of primate species. here, we report on the milk composition from laboratory-housed bo ...200817538959
spontaneous yersiniosis due to yersinia pseudotuberculosis serotype 7 in a squirrel monkey.a captive male bolivian squirrel monkey (saimiri boliviensis) of less than 1 year of age died following diarrhea and debilitation on the day of death. at necropsy, necrotizing enteritis accompanied with enlarged peyer's patches, solitary lymphatic follicles and mesenteric lymph nodes, and multiple yellowish-white nodules in the spleen and liver were found. histopathologically, these lesions were necrotizing inflammation containing gram-negative bacilli. yersinia pseudotuberculosis serotype 7 was ...200920046036
milk composition of captive tufted capuchins (cebus apella).little is known about the milk composition of nonhuman primates, and it has never been examined in capuchin monkeys (genus cebus). this article reports on the macronutrient milk composition (fat, crude protein (cp), lactose, dry matter (dm), and total gross energy (ge)) of captive housed tufted capuchins (cebus apella) (n=8). c. apella milk averaged 5.22% fat, 2.40% cp, 6.94% lactose, 16.48% dm, and 0.89 kcal/g. fat was the most variable macronutrient and was significantly higher in samples coll ...201019827136
chemical characterization of oligosaccharides in the milk of six species of new and old world monkeys.human and great ape milks contain a diverse array of milk oligosaccharides, but little is known about the milk oligosaccharides of other primates, and how they differ among taxa. neutral and acidic oligosaccharides were isolated from the milk of three species of old world or catarrhine monkeys (cercopithecidae: rhesus macaque (macaca mulatta), toque macaque (macaca sinica) and hamadryas baboon (papio hamadryas)) and three of new world or platyrrhine monkeys (cebidae: tufted capuchin (cebus apell ...201021127965
phenotypic and functional characterization of lymphocytes from different age groups of bolivian squirrel monkeys (saimiri boliviensis boliviensis).due to many physiological and genetic characteristic similarities to humans, squirrel monkeys provide an ideal animal model specifically for studying malaria, and transmissible spongiform encephalopathies (creutzfeldt-jacob disease). while squirrel monkeys three years and older are generally considered adult subjects suitable for use in medical research studies, little is known about the functional properties of lymphocytes in relation to the age of these animals, which could significantly impac ...201324282512
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