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genomic diversity among corynebacterium jeikeium strains and comparison with biochemical characteristics and antimicrobial susceptibilities.levels of dna relatedness were determined by performing dna-dna hybridization experiments (s1 nuclease procedure) with 13 human isolates exhibiting various antimicrobial susceptibility patterns which had been identified as corynebacterium jeikeium by classical tests and the api coryne system and with reference strains of c. jeikeium and related taxa. twelve of 13 isolates which formed three genomic groups showed between 22 and 75% relatedness with the type strain of c. jeikeium. one of these gen ...19947989533
evaluation of the applicability of amplified rdna-restriction analysis (ardra) to identification of species of the genus corynebacterium.the 16s rrna genes (rdna) of 50 strains belonging to 26 different coryneform bacterial species and genomospecies and of the type strain of rhodococcus equi were enzymatically amplified. amplified rdna restriction analysis (ardra) with the enzymes alui, cfoi and rsai was carried out. the combination of the ardra patterns obtained after restriction with these three different enzymes enabled the differentiation between the following species: corynebacterium accolens (number of strains = 2), c. afer ...19958584787
genomic diversity and phylogenetic relationships among lipid-requiring diphtheroids from humans and characterization of corynebacterium macginleyi sp. nov.dna relatedness experiments were performed with 38 clinical isolates and 13 reference strains of coryneform taxa exhibiting a lipid requirement for optimal growth. forty-five of these strains split into five genomic groups at the species level, whereas six other strains remained unclustered. genomospecies ii fits corynebacterium accolens, but the other genomospecies were not genetically related to any of the defined corynebacterium species. phylogenetic analyses of genes coding for small-subunit ...19957857793
endocarditis of native aortic and mitral valves due to corynebacterium accolens: report of a case and application of phenotypic and genotypic techniques for identification.endocarditis of native aortic and mitral valves due to an organism identified as corynebacterium accolens developed in a 73-year-old patient without predisposing factors. the organism was identified as c. accolens by biochemical identification, amplified rrna gene restriction analysis, and dna-dna hybridization. this is the first case of c. accolens endocarditis reported, adding to the increasing number of corynebacterium-related cases of endocarditis.19968727922
species identities and antimicrobial susceptibilities of corynebacteria isolated from various clinical sources.over a 14-month period, 415 clinical isolates of coryneform gram-positive rods were recovered from various sources and identified to the species level according to recent identification schemes. corynebacterium urealyticum, corynebacterium striatum, corynebacterium amycolatum, and corynebacterium jeikeium predominated, accounting for 63% of all isolates. corynebacterium accolens, corynebacterium striatum, corynebacterium argentoratense, corynebacterium propinquum and corynebacterium pseudodiphth ...19968894575
corynebacterium accolens isolated from breast abscess: possible association with granulomatous mastitis.corynebacterium accolens is rarely isolated as a human pathogen. we describe here a case of c. accolens isolated from a breast abscess in a patient previously diagnosed with granulomatous mastitis. the possible association of corynebacterium accolens and granulomatous mastitis in this patient is discussed.200717344355
corynebacterium timonense sp. nov. and corynebacterium massiliense sp. nov., isolated from human blood and human articular hip fluid.gram-positive, facultatively anaerobic, rod-shaped bacteria were isolated from the blood of a patient with endocarditis (strain 5401744t) and from the hip joint fluid of a patient with an infected orthopaedic prosthesis (strain 5402485t). these strains were characterized by using a polyphasic taxonomic approach. based on cellular morphology and biochemical criteria the two isolates were tentatively assigned to the genus corynebacterium, although they did not correspond to any recognized species. ...200919567562
corynebacterium accolens-associated pelvic osteomyelitis.corynebacterium accolens is a rare human pathogen. we encountered a case of c. accolens isolated from a thigh collection in a man with osteomyelitis of the adjacent pubic symphysis.201020032254
microbial contamination of autologous peripheral blood stem cell products: incidence, clinical outcome, quality control and management strategies.: microbial screening is part of quality control for autologous peripheral blood stem cell (pbsc) manipulation. the incidence of culture positive products varies widely. the clinical significance of infusing culture positive products is uncertain, with no consensus on the interventions required.201121532522
microbiological investigation and clinical significance of corynebacterium spp. in respiratory specimens.the purpose of this retrospective study was to evaluate the pathogenic role of corynebacterium species in lower respiratory tract infections as well as their routine laboratory investigation. from april 2007 to august 2009, 27 clinical isolates were significantly recovered from respiratory specimens of 27 different patients clinically suspected of having lower respiratory tract infections. the average age of patients was 65 years, while 22 (81%) patients presented at least 1 predisposing conditi ...201222938828
nasal microenvironments and interspecific interactions influence nasal microbiota complexity and s. aureus carriage.the indigenous microbiota of the nasal cavity plays important roles in human health and disease. patterns of spatial variation in microbiota composition may help explain staphylococcus aureus colonization and reveal interspecies and species-host interactions. to assess the biogeography of the nasal microbiota, we sampled healthy subjects, representing both s. aureus carriers and noncarriers at three nasal sites (anterior naris, middle meatus, and sphenoethmoidal recess). phylogenetic composition ...201324331461
polymicrobial and microsporidial keratitis in a patient using boston scleral contact lens for sjogren's syndrome and ocular cicatricial pemphigoid.to report a rare case of microsporidial and polymicrobial keratitis in a patient with sjogren's syndrome and ocular cicatricial pemphigoid.201323123433
patient nostril microbial flora: individual-dependency and diversity precluding prediction of staphylococcus aureus acquisition.the potential role of a patient's resident microbial flora in the risk of acquiring multiresistant bacteria (mrb) during hospitalization is unclear. we investigated this role by cross-sectional study of 103 patients at risk of acquisition of staphylococcus aureus (sa), resistant (mrsa) or not (mssa) to methicillin, recruited in four french hospitals. the flora was analysed by an exhaustive culture-based approach combined with molecular and/or mass-spectrometry-based identification, and sa strain ...201423601162
unraveling the molecular mechanisms underlying the nasopharyngeal bacterial community structure.the upper respiratory tract is colonized by a diverse array of commensal bacteria that harbor potential pathogens, such as streptococcus pneumoniae. as long as the local microbial ecosystem-also called "microbiome"-is in balance, these potentially pathogenic bacterial residents cause no harm to the host. however, similar to macrobiological ecosystems, when the bacterial community structure gets perturbed, potential pathogens can overtake the niche and cause mild to severe infections. recent stud ...201626838716
corynebacterium accolens releases antipneumococcal free fatty acids from human nostril and skin surface triacylglycerols.bacterial interspecies interactions play clinically important roles in shaping microbial community composition. we observed that corynebacterium spp. are overrepresented in children free of streptococcus pneumoniae (pneumococcus), a common pediatric nasal colonizer and an important infectious agent. corynebacterium accolens, a benign lipid-requiring species, inhibits pneumococcal growth during in vitro cocultivation on medium supplemented with human skin surface triacylglycerols (tags) that are ...201626733066
development of the nasopharyngeal microbiota in infants with cystic fibrosis.cystic fibrosis (cf) is characterized by early structural lung disease caused by pulmonary infections. the nasopharynx of infants is a major ecological reservoir of potential respiratory pathogens.201626492486
corynebacterium species nasopharyngeal carriage in asymptomatic individuals aged ≥ 65 years in germany.the prevalence of protective anti-diphtheria toxin antibodies decreases with age. therefore, the elderly might serve as reservoir for potentially toxigenic corynebacterium (c.) species (c. diphtheriae, c. ulcerans, and c. pseudotuberculosis). this study aimed to examine the colonization rate of the nasopharynx with corynebacteria of individuals aged 65 years and older.201728429151
contextual control of skin immunity and inflammation by corynebacterium.how defined microbes influence the skin immune system remains poorly understood. here we demonstrate that corynebacteria, dominant members of the skin microbiota, promote a dramatic increase in the number and activation of a defined subset of γδ t cells. this effect is long-lasting, occurs independently of other microbes, and is, in part, mediated by interleukin (il)-23. under steady-state conditions, the impact of corynebacterium is discrete and noninflammatory. however, when applied to the ski ...201829382696
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