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[comparative study on six bacterial anaerobic non-sporulated strains: actinomyces bovis (bovine), actinomyces bovis (human), actinomyces israeli var. liquefaciens, corynebacterium acnes, corynebacterium avidum and corynebacterium liquefaciens]. 195313148703
[osler's disease due to corynebacterium liquefaciens]. 195413160810
[osteoarticular tuberculosis of malar; presence of corynebacterium liquefaciens; discussion]. 195613380089
[corynebacterium liquefaciens abscesses of the brain complicating fallot's pentalogy cured by successive neurosurgical operations]. 195613390246
[experimental inoculation of the chick embryo with corynebacterium liquefaciens]. 196113884992
occurrence of nucleotides in culture fluids of microorganisms. iii. nucleotide excretion by brevibacterium liquefaciens sp. n. with special reference to uridine diphospho-n-acetylglucosamine compounds.okabayashi, t. (shionogi research laboratory, fukushimaku, osaka, japan). occurrence of nucleotides in culture fluids of microorganisms. iii. nucleotide excretion by brevibacterium liquefaciens sp. n. with special reference to uridine diphospho-n-acetylglucosamine compounds. j. bacteriol. 84:1-8. 1962.-among various nucleotides excreted in the culture broth of brevibacterium liquefaciens sp. n., four uridine nucleotides containing n-acetylglucosamine were isolated. these were found to be uridine ...196214481473
excretion of adenosine-3',5'-phosphate in the culture broth of brevibacterium liquefaciens. 196313939741
occurrence of nucleotides in culture fluids of microorganisms. v. excretion of adenosine cyclic 3',5'-phosphate by brevibacterium liquefaciens sp. n.okabayashi, tadashi (shionogi & co., ltd., fukushima-ku, osaka, japan), akihiro yoshimoto, and misao ide. occurrence of nucleotides in culture fluids of microorganisms. v. excretion of adenosine cyclic 3',5'-phosphate by brevibacterium liquefaciens sp. n. j. bacteriol. 86:930-936. 1963.-brevibacterium liquefaciens sp. n., when grown in a medium containing amino acids as the nitrogen source, excreted a considerable amount of an adenine ribonucleotide, which had not previously been noticed. the nu ...196314080803
pyruvate dependent adenyl cyclase activity of brevibacterium liquefaciens. 19655865503
formation of adenosine cyclic 3',5'-phosphate by nonproliferating cells and cell-free extract of brevibacterium liquefaciens.the formation of adenosine cyclic 3',5'-phosphate by brevibacterium liquefaciens atcc 14929 was studied with the use of nonproliferating cells and cell-free extract. with nonproliferating cells provided by deprivation of sulfate, the formation of this nucleotide was accelerated by adding some amino acids and sugars. among amino acids tested, alanine and asparagine were most effective. pentoses were more favorable than hexoses and other sugars. formation of adenosine cyclic 3',5'-phosphate was ob ...19676039354
adenyl cyclase of brevibacterium liquefaciens. 19674295782
pyruvate kinase of brevibacterium liquefaciens. 19705472682
reversible inactivation of phosphofructokinase from brevibacterium liquefaciens. 19714259268
adenylate cyclase of brevibacterium liquefaciens. inactivation by neuraminidase, phospholipase a and phospholipase c. 19734783345
[corynebacterium liquéfaciens septicemia]. 19734794301
adenylate cyclase from brevibacterium liquefaciens. i. purification, crystallization, and some properties. 19744817972
adenylate cyclase from brevibacterium liquefaciens. reversibility and thermodynamic studies. 19744846750
adenylate cyclase from brevibacterium liquefaciens. 19744453188
adenylate cyclase from brevibacterium liquefaciens. iii. in situ regulation of adenylate cyclase by pyruvate.in the presence of dl-alanine intracellular cyclic amp in nonproliferating cells of brevibacterium liquefaciens increased rapidly to the maximum level of approximately 180 mum, and extracellular cyclic amp increased to 100 mum within 4 hr at 25 degrees . adenylate cyclase (ec 4.6.1.1) induction was not observed during this incubation. the concentration of pyruvate in the total culture increased concomitantly with that of cyclic amp and reached approximately 20 mm after 4 hr of incubation. since ...19744373721
combination of non-specific immunopotentiation with corynebacterium liquefaciens and chemotherapy in tumor-bearing mice.the effect of combining chemotherapeutics (mitomycin-c, cyclophosphamide, or 5-fluorouracil) and immunopotentiator (anaeobic corynebacterium liquefaciens) against ehrlich ascites carcinoma in mice was examined. mitomycin-c and cyclophosphamide were given intraperitoneally 2 days after the inoculation of tumor cells. 5-fluorouracil was administered intraperitoneally for 7 consecutive days beginning from the second day after inoculation of tumor cells. c. liquefaciens was given intraperitoneally i ...19751225723
brevibacterium liquefaciens adenylate cyclase and its in vivo stimulation by pyruvate.adenylate cyclase of brevibacterium liquefaciens depends on pyruvate for activity. growing in a simple medium containing glucose and dl-alanine, the microorganism excreted pyruvate, which reached 20 mm in the medium at stationary phase. using [3h]adenosine to label the adenosine 5'-triphosphate pool, we showed that pyruvate in the medium stimulated adenylate cyclase of b. liquefaciens in vivo, in a manner similar to the stimulation observed in vitro. adenylate cyclase in cells harvested at diffe ...1975172486
effect of anti-thymocyte serum, anti-macrophage serum, and latex particles on the therapeutic efficacy of bcg or corynebacterium liquefaciens (propionibacterium acnes c7) in syngeneic mice.in a syngeneic mouse-tumor system, anti-thymocyte serum, anti-macrophage serum, and latex particles were used for a comparative study of the immunotherapeutic efficacy of intradermal inoculation of tumor cell-bcg and tumor cell-corynebacterium liquefaciens (=propionibacterium acnes c7) vaccines. anti-thymocyte serum treatment nullified suppression of tumor mediated by bcg (living and nonliving) and living c. liquefaciens. however, the effect of nonliving c. liquefaciens was not affected so much ...1977324858
combined treatment with anaerobic corynebacterium liquefaciens and chemotherapeutics against solid tumor in mice.the combined effect of anticancer agents (mitomycin-c, cyclophosphamide, or 5-fluorouracil) and anaerobic corynebacterium liquefaciens on subcutaneously induced solid ehrlich carcinoma in mice was examined. mitomycin-c and cyclophosphamide were given intraperitoneally on day 7 after inoculation of tumor cells. 5-fluorouracil was administered intraperitoneally for 7 consecutive days from day 9 to 15. c. liquefaciens was given in two ways, intraperitoneally and intratumorally. its injections were ...1977863171
comparative study of corynebacterium parvum and corynebacterium liquefaciens on antitumor activity against sarcoma-180.corynebacterium parvum and corynebacterium liquefaciens were comparatively examined for their antitumor activity against sarcoma-180 in ddy mice. in the case of ascitic form, significant antitumor effect was observed when c. parvum was administered on days -4 and -2 or day -2. as for solid form, maximal effect was obtained when c. parvum was administered on day 0. on the other hand, c. liquefaciens exhibited maximal antitumor activity against sarcoma-180, both in ascitic and solid forms, when it ...1977892298
[experimental studies on the antitumor activity of corynebacterium liquefaciens]. 1977276194
effect of corynebacterium liquefaciens on a c3hf mouse squamous cell carcinoma.the antitumor effect of anaerobic corynebacterium liquefaciens was compared with that of specific immunization. experimental tumors were fourth or fifth generation isotransplants of a nr-sl squamous cell carcinoma that arose spontaneously in a c3hf/he female mouse. specific immunization failed to exhibit an antitumor effect, whereas a single administration of the bacterium markedly inhibited the growth of the tumor. this growth inhibition was most effective when c. liquefaciens was administered ...1977884666
antitumor activity of macrophages induced by corynebacterium liquefaciens.corynebacterium liquefaciens can induce a marked resistance to tumor cell (ah-7974) challenge in donryu rats. this effect was also induced in t-cell-deficient athymic nude mice. the macrophages harvested from the peritoneal cavity of a rat injected with c. liquefaciens showed a marked inhibition of tumor growth in vitro. therefore, the effector cells were assumed to be peritoneal macrophages. the time needed for activation of macrophages in vivo was within 3 to 5 days after injection of c. lique ...1977616415
cytocidal and cytostatic ability of corynebacterium liquefaciens in mouse squamous cell carcinoma in vivo.the cytostatic and cytocidal action of corynebacterium liquefaciens was studied in a mouse squamous cell carcinoma in vivo. kinetic analysis of tumor cells 4 and 8 days after a single i.p. dose of 2.0 mg c. liquefaciens showed a marked prolongation of the mean cell generation time (tg). this prolongation affected most the g1 and, to a lesser extent, the s phases of the cell cycle. the tumor growth factor was decreased, and the mean value of the cell loss factor was increased. assays to determine ...1978647686
steady-state kinetic studies on adenylate cyclase from brevibacterium liquefaciens. 1978639814
cytotoxic activity of human blood monocytes against cultured breast cancer cells.cytotoxic activity of blood monocytes from 19 healthy individuals was examined in vitro against the cultured floating cells of human breast cancer origin. target tumor cells inoculated on monocyte cultures and maintained for 5 days were reduced in number, compared with the control cultues of tumor cells alone. increased cytotoxicity of the monocytes was noted after in vitro activation by heat-killed corynebacterium liquefaciens (propionibacterium acnes). c. liquefaciens of the same dose as used ...1978680464
comparison of the in-vitro antitumor activity of peritoneal cells from mice injected with corynebacterium parvum and corynebacterium liquefaciens. 1979478988
corynebacterium liquefaciens-induced suppressor t cells in graft-versus-host reaction.the effect of the spleen cells and thymus cells from corynebacterium liquefaciens-treated (c57bl/6 x balb/c)f1 hybrid mice on graft-versus-host (gvh) reaction, initiated in the parental to newborn f1 hybrid strain combination, was studied by using simonsen's spleen assay. the combined inocula of 5 x 10(6) normal balb/c spleen cells and 50 x 10(6) spleen cells from f1 mice that had been given i.v. injections of 1 mg of c. liquefaciens yielded a significantly smaller spleen index than did 5 x 10(6 ...19806966090
mechanism of the adenylate cyclase reaction. stereochemistry of the reaction catalyzed by the enzyme from brevibacterium liquefaciens.adenylate cyclase from brevibacterium liquefaciens (atcc 14929) catalyzes the formation of the rp-diastereomer of adenosine 3':5'-cyclic monophosphorothioate from the sp-diastereomer of adenosine-5'-(1-thiotriphosphate). the reaction catalyzed by this adenylate cyclase proceeds with inversion of configuration at phosphorus, indicating that the cyclization reaction is direct and does not involve formation of an adenylated enzyme intermediate.19806243273
taxonomic studies on arthrobacter nicotianae and related taxa: description of arthrobacter uratoxydans sp. nov. and arthrobacter sulfureus sp. nov. and reclassification of brevibacterium protophormiae as arthrobacter protophormiae comb. nov.dna homology studies were performed on various strains of arthrobacter and related organisms with peptidoglycans (mureins) of the a3α and a4α variations. homology values above 20% show strains with the a4α variation to form a coherent group, which is here designated as the "nicotianae" group of organisms. members of this group show homology values of lower than 20% with strains of the "globiformis" group (arthrobacter globiformis and relatives), having a a3α peptidoglycan variation. however, the ...198323194805
augmentation by corynebacterium liquefaciens of erythrocyte surface h-2 expression and alloimmunogenicity for antibody responses.intravenous injection of killed corynebacterium liquefaciens induced a population of red blood cells that expressed both h-2k and h-2d antigens at exceptionally high density and displayed augmented immunogenicity for h-2 alloantigen-specific b cell activation. injection of killed escherichia coli or e. coli lipopolysaccharide was ineffective for the generation of such rbc. rbc that express h-2 antigens at high density first appeared at 7 days after injection of c. liquefaciens. these rbc persist ...19883261464
augmented production of granulocyte-macrophage colony-stimulating factor and alpha/beta interferon in mice inoculated with heat-killed corynebacterium liquefaciens.we demonstrated that heat-killed corynebacterium liquefaciens bacteria, as a known potent host immune activity modulator, stimulate spleen cells to produce granulocyte-macrophage (gm) colony-stimulating factor (csf) and another csf with similar activity, as well as alpha/beta interferon, when injected intravenously into mice. alpha/beta interferon was shown to be produced by c. liquefaciens-activated plastic-g-10 column-adherent cells (a cells) in a thymus-independent manner. in contrast, augmen ...19911997406
a pyruvate-stimulated adenylate cyclase has a sequence related to the fes/fps oncogenes and to eukaryotic cyclases.the pyruvate-stimulated adenylate cyclase from brevibacterium liquefaciens produces up to 450 microm cyclic amp in the culture medium when the bacterium is grown on glucose and alanine. in this paper we report the cloning, expression and sequencing of the gene for this enzyme. residues were identified, within the c-terminal domain, which are conserved in adenylate and guanylate cyclase sequences from eukaryotes and in the adenylate cyclase of the prokaryote rhizobium meliloti. we have also ident ...19911683468
cellular fatty acid composition as an adjunct to the identification of asporogenous, aerobic gram-positive rods.cellular fatty acid (cfa) compositions of 561 asporogenous, aerobic gram-positive rods were analyzed by gas-liquid chromatography as an adjunct to their identification when grown on blood agar at 35 degrees c. the organisms could be divided into two groups. in the first group (branched-chain type), which included coryneform cdc groups a-3, a-4, and a-5; some strains of b-1 and b-3; "corynebacterium aquaticum"; brevibacterium liquefaciens; rothia dentocariosa; and listeria spp., the rods had siza ...19911899679
antagonistic effect of coryneform bacteria from red smear cheese against listeria species.a total of 187 coryneform bacteria were isolated from red smear and screened for inhibitory effects against 16 strains of listeria species. culture filtrates from brevibacterium linens (16 strains), arthrobacter nicotianae (4 strains) and arthrobacter nucleogenes (3 strains) showed clear zones of inhibition. the antagonistic effect was seen against 26 to 87% of 91 listeria strains tested. a. nicotianae and a. nucleogenes were more effective against listeria innocua and listeria ivanovii than aga ...19911909545
[effect of normal and specific immune sera on neuraminidase activity].we have got evidence that there is no antigenic relationship reflecting the structural similarity between neuraminidases synthesized by noncholera vibrios and arthrobacter nicotianae. the cross-reactions between the enzymes and heterological antisera were not observed. antibodies against the a. nicotianae neuraminidase inhibited the activity of the enzyme for a glycomacropeptide of milk whey and for components of the blood serum, and had no effect no the neuraminidase from noncholera vibrios. an ...19921528820
the adenylate cyclase catalytic domain of streptomyces coelicolor is carboxy-terminal.a dna fragment of streptomyces coelicolor encoding the carboxy-terminal catalytic domain of adenylate cyclase was cloned, sequenced and expressed in an escherichia coli cya-defective strain where it produced nanomole levels of camp. the amino acid sequence of the enzyme displays similarities with the brevibacterium liquefaciens pyruvate regulated adenylate cyclase.19938282183
uptake and utilization of n-octacosane and n-nonacosane by arthrobacter nicotianae kcc b35.arthrobacter nicotianae kcc b35 isolated from blue-green mats densely covering oil sediments along the arabian gulf coast grew well on c10 to c40 n-alkanes as sole sources of carbon and energy. growth on c20 to c40 alkanes was even better than on c10 to c18 alkanes. biomass samples incubated for 6 h with n-octacosane (c28) or n-nonacosane (c29) accumulated these compounds as the predominant constituent alkanes of the cell hydrocarbon fractions. the even chain hexadecane c16 and the odd chain pen ...19968849639
microbial biosensor for free fatty acids using an oxygen electrode based on thick film technology.a microbial biosensor based on thick film technology was developed. the microorganisms, arthrobacter nicotianae, were immobilized in ca-alginate directly on the electrode surface. for the stability of the calcium alginate gel the addition of 0.5 mm cacl2 to the assay buffer was necessary. the respiratory activity of the microorganisms was monitored by oxygen consumption at -600 mv vs. ag/agcl reference electrode. the sensor was used in a batch system and was applied to the determination of free ...19968828165
rapid physiological characterization of microorganisms by biosensor technique.eleven microorganisms, arxula adeninivorans ls3, candida boidinii dsm 70034, candida lactis-condensi dsm 70635, pichia jadinii dsm 2361, pichia minuta dsm 7018, kluyveromyces lactis dsm 4394, pseudomonas putida dsm 50026, alcaligenes sp. dsm 30002, arthrobacter nicotianae dsm 20123 as well as issatchenkia orientalis dsm 70077 and rhodococcus erythropolis dsm 311 were characterized by the sensor technique by injection of 30 different substrates and substrate mixtures. the obtained data which are ...19979352658
identification of yeasts and coryneform bacteria from the surface microflora of brick cheeses.coryneform bacteria and yeasts of 21 brick cheeses from six german dairies, produced by using undefined ripening cultures, were identified. arthrobacter nicotianae, brevibacterium linens, corynebacterium ammoniagenes, corynebacterium variabilis and rhodococcus fascians were found in significant numbers. out of 148 coryneform isolates 36 could not be identified at the species level. with the exception of a large rennet cheese, the coryneform microflora of rennet and acid cured cheeses were simila ...19979039559
the eff-482 locus of sinorhizobium meliloti cxm1-105 that influences symbiotic effectiveness consists of three genes encoding an endoglycanase, a transcriptional regulator and an adenylate cyclase.the mutant t482 of sinorhizobium meliloti cxm1-105, which carries a tn5 insertion on megaplasmid 1, exhibits an enhanced symbiotic efficiency phenotype. three genes, eglc, cya3 and syrb2, were identified in the eff-482 region tagged by the tn5 insertion in t482. the eglc gene encodes an endoglycanase which contributes to the depolymerization of the exo-polysaccharide succinoglycan. the n-terminal region of the predicted cya3 gene product was similar to eukaryotic-type adenylate cyclases from bre ...199910485295
purification and characterization of an extracellular proline iminopeptidase from arthrobacter nicotianae 9458.an extracellular proline iminopeptidase, with a molecular mass of about 53 kda, was purified from arthrobacter nicotianae 9458 and characterized. the enzyme had temperature and ph optima of 37 degrees c and 8.0, respectively, was completely inactivated by heating for 1 min at 80 degrees c and showed highest activity on pro-pna. the proline iminopeptidase was characterized by activity at low temperature, nacl concentrations up to 7.5% and by high sensitivity to ph values 6.0, serine enzyme inhibi ...199910483739
purification and characterization of two extracellular proteinases from arthrobacter nicotianae 9458.two extracellular serine proteinases with molecular masses of about 53-55 and 70-72 kda, were purified from arthrobacter nicotianae 9458 and characterized. the enzymes differed with respect to temperature optimum, 55-60 and 37 degrees c, respectively, tolerance to low values of ph and temperature, heat stability, sensitivity to edta and sulfhydryl blocking agents, and hydrophobicity. both proteinases were optimally active in the ph range of 9.0 and 9.5, had considerable activity at ph 6.0 on alp ...19999933928
effects of lipids on n-alkane attenuation in media supporting oil-utilizing microorganisms from the oily arabian gulf coasts.the arabian gulf is one of the most extensively oil-polluted areas of the world. the major objectives of this work were to study whether hydrocarbon-utilizing microorganisms indigenous to that area would readily accumulate added lipids, and whether this might affect their hydrocarbon consumption potential. two prokaryotes, arthrobacter nicotianae kcc b35 and the unidentified organisms kcc b6, as well as one eukaryote, candida parapsilosis kcc y1, were selected for this study. biomass samples of ...200111430415
novel reversible indole-3-carboxylate decarboxylase catalyzing nonoxidative decarboxylation.after enrichment culture with indole-3-carboxylate in static culture, a novel reversible decarboxylase, indole-3-carboxylate decarboxylase, was found in arthrobacter nicotianae fi1612 and several molds. the enzyme reaction was examined in resting-cell reactions with a. nicotianae fi1612. the enzyme activity was induced specifically by indole-3-carboxylate, but not by indole. the indole-3-carboxylate decarboxylase of a. nicotianae fi1612 catalyzed the nonoxidative decarboxylation of indole-3-carb ...200212506977
electron spin resonance study of copper biosorption by bacteria.the biosorption of copper by bacteria was studied by using esr spectroscopy. among bacteria tested, arthrobacter nicotianae has the most excellent ability to sorb copper. the biosorption of copper by arthrobacter cells was so rapid, affected by the solution ph, and obeys the langmuir adsorption equilibrium. the electron spin resonance (esr) spectra of cu(ii) in bacterial cells are axial type, having a major absorption to higher field at gperpendicular and lesser absorption to lower field at gpar ...200212092584
removal and recovery of uranyl ion using various microorganisms.the adsorption of uranyl ion by microorganisms was examined. among the 76 strains of 69 species tested (23 bacteria, 20 actinomycetes, 18 fungi, and 15 yeasts), high uranyl ion adsorption ability was exhibited by strains of the bacteria, arthrobacter nicotianae, bacillus subtilis, and micrococcus luteus. a. nicotianae cells, which showed the best performance, could adsorb about 698 mg uranyl ion (2.58 mmol) per gram dry wt. of microbial cells. the adsorption of uranyl ion was rapid, selective, a ...200216233264
members of the genus arthrobacter grow anaerobically using nitrate ammonification and fermentative processes: anaerobic adaptation of aerobic bacteria abundant in soil.members of the genus arthrobacter are usually regarded as obligate aerobic bacteria. the anaerobic growth and energy metabolism of two arthrobacter species were investigated. arthrobacter globiformis utilized both nitrate ammonification and lactate, acetate and ethanol producing fermentation processes for anaerobic growth. only nitrate supported anaerobic growth of arthrobacter nicotianae. anaerobically induced respiratory nitrate reductase activity was detected in both strains. neither of the t ...200312829291
reclassification of brevibacterium liquefaciens okabayashi and masuo 1960 as arthrobacter nicotianae giovannozzi-sermanni 1959.brevibacterium liquefaciens atcc 14929(t) was reclassified as corynebacterium liquefaciens by lanéelle et al. (1980). a further study by stackebrandt et al. (1983) described the same strain, indicating high genomic and chemotaxonomic relatedness to arthrobacter nicotianae; however, reclassification was not formally proposed. because of the discrepancies between their previous work and the data of collins & kroppenstedt (1983), lanéelle and colleagues re-examined b. liquefaciens and withdrew thei ...200415023984
purification and characterization of a novel sialidase from a strain of arthrobacter nicotianae.the nonpathogenic strain arthrobacter nicotianae produces two sialidase isoenzymes, na1 and na2, with molecular masses of 65 kda and 54 kda, respectively, as determined by 10% sds-polyacrylamide gel electrophoresis. na1 and na2 exhibit maximum activities at ph 4 and 5, and both show clear thermal optima at 40 degrees c. they are stable at temperatures up to 50 degrees c. the critical temperatures (t (c) = 50 degrees c and 51 degrees c) for the two isoenzymes were determined by fluorescence spect ...200515809338
arthrobacter bergerei sp. nov. and arthrobacter arilaitensis sp. nov., novel coryneform species isolated from the surfaces of cheeses.fourteen isolates of two different bacterial species isolated from the surface of smear-ripened cheeses were found to exhibit many characteristics of the genus arthrobacter. the isolates were aerobic, gram-positive, catalase-positive, non-spore-forming and non-motile. the cell-wall peptidoglycan contained lysine, alanine and glutamic acid. rrs sequence analysis indicated that the new isolates re117t and ca106t are closely related to the arthrobacter nicotianae group and showed highest sequence s ...200515653918
removal and recovery of lithium using various microorganisms.the accumulation of lithium by microorganisms was examined. among the 70 strains of the 63 species tested (20 bacteria, 18 actinomycetes, 18 fungi, and 14 yeasts), a high lithium accumulating ability was exhibited by strains of the bacteria, arthrobacter nicotianae and brevibacterium helovolum. lithium accumulation by a. nicotianae cells was strongly affected by the ph of the solution. the amount of accumulated lithium was maximum at ph 6. cells immobilized with polyacrylamide gel also adsorbed ...200516384797
selective accumulation of light or heavy rare earth elements using gram-positive bacteria.the accumulation of samarium from a solution only containing samarium by arthrobacter nicotianae was examined. the amount of accumulated samarium was strongly affected by the concentration of samarium and ph of the solution. the accumulation of samarium by the strain was very rapid and reached equilibrium within 3h. the accumulation of samarium-europium or europium-gadolinium from the solution containing the two metals using various actinomycetes and gram-positive bacteria was also examined. mos ...200616797944
biochemical evidence for the proteolytic degradation of infectious prion protein prpsc in hamster brain homogenates by foodborne bacteria.prp(sc) is a general term to describe the infectious agent causing transmissible spongiform encephalopathy (tse), and the protease-resistant form of cellular prp(c). in this study, we have identified several protease-secreting bacteria able to degrade prp(sc) under more or less native conditions (30 degrees c, ph 8), focusing on strains isolated mainly from cheese. one hundred and ninty-nine protease-secreting isolates belonging to the actinomycetales and bacillales were screened for the express ...200616464698
[biosynthetic features and properties of xylose isomerases from arthrobacter nicotianae, escherichia coli, and erwinia carotovota subsp. atroseptica].the characteristics of xylose isomerase biosynthesis in the bacteria arthrobacter nicotianae bim b-5, erwinia carotovota subsp atroseptica jn42xyla, and escherichia coli hb101xyla have been studied. the bacteria formed the enzyme constitutively. out of the carbon sources studied, d-glucose and d-xylose were most favorable for the biosynthesis of xylose isomerase in e. carotovota subsp atroseptica, but the least appropriate in terms of the enzyme production efficiency in e. coli. minimum and maxi ...200616878542
[glucose isomerase activity in suspension of arthrobacter nicotianae cells and adsorption immobilization of the microorganisms on inorganic carriers].kinetics of monosaccharide isomerization has been studied in suspensions of intact, non-growing arthrobacter nicotianae cells. under the conditions of the study, glucose and fructose were isomerized at the same maximum rate of 700 micromol/min per 1 g dried cells, which increased with temperature (the dependence was linear at 60-80 degrees c). the proposed means of adsorption immobilization of a. nicotianae cells involve inorganic carriers differing in macrostructure, chemical nature, and surfac ...200818669262
heterologous expression and characterization of choline oxidase from the soil bacterium arthrobacter nicotianae.in the course of a microbial screening of soil samples for new oxidases, different enrichment strategies were carried out. with choline as the only carbon source, a microorganism was isolated and identified as arthrobacter nicotianae. from this strain, a gene coding for a choline oxidase was isolated from chromosomal dna. this gene named coda was cloned in escherichia coli bl21-gold and the protein (an_coda) heterologously overexpressed as a soluble intracellular protein of 59.1 kda. basic bioch ...200918787818
eric-pcr-based strain-specific detection of phenol-degrading bacteria in activated sludge of wastewater treatment systems.to evaluate the use of enterobacterial repetitive intergenic consensus pcr (eric-pcr)-derived probes and primers to specifically detect bacterial strains in an activated sludge microbial community.200919708883
[catalytical properties of arthrobacter nicotianae cells, a producer of glucose isomerase, immobilized in xerogel of silicium dioxide].arthrobacter nicotinanae cells, producers of glucose isomerase, were immobilized in xerogel of silicium dioxide, and properties of the resulted heterogeneous biocatalysts were investigated in the process of isomerization of monosaccharide (glucose and fructose). the glucose isomerase activity of the resulted biocatalysts was shown to be 10 u/g, on average, taking into account the loss of the activity upon the immobilization, which amounted to 50% of the cell activity in suspension. the rate of t ...200919764612
maturing dynamics of surface microflora in fontina pdo cheese studied by culture-dependent and -independent methods.to study the evolution of rind microbial communities in fontina pdo cheese.200919054234
biodegradation of phenanthrene and pyrene in compost-amended soil.this study investigated the biodegradation of the polycyclic aromatic hydrocarbons (pahs) phenanthrene and pyrene in compost and compost-amended soil. the degradation rates of the two pahs were phenanthrene>pyrene. the degradation of pah was enhanced when the two pahs were present simultaneously in the soil. the addition of either of the two types of compost (straw and animal manure) individually enhanced pah degradation. compost samples were separated into fractions with various particle size r ...200919412845
engineering of choline oxidase from arthrobacter nicotianae for potential use as biological bleach in detergents.in order to engineer the choline oxidase from arthrobacter nicotianae (an_coda) for the potential application as biological bleach in detergents, the specific activity of the enzyme toward the synthetic substrate tris-(2-hydroxyethyl)-methylammonium methylsulfate (mtea) was improved by methods of directed evolution and rational design. the best mutants (up to 520% wt-activity with mtea) revealed mutations in the fad- (a21v, g62d, i69v) and substrate-binding site (s348l, v349l, f351y). in a separ ...201020467737
[xylose isomerase constitutive biosynthesis in arthrobacter nicotianae].the specific features of biosynthesis of the cell-bound xylose isomerase by the actinobacterium arthrobacter nicotianae bim v-5 were studied. it was demonstrated that the constitutive synthesis of this enzyme in the studied bacteria, not subject to catabolite repression, was inhibited by xylulose, an intermediate product ofxylose utilization and the final product of its enzymatic isomerization. short-term experiments demonstrated that xylulose at a concentration of 0.005% almost completely repre ...201020873168
characterization of three antifungal calcite-forming bacteria, arthrobacter nicotianae knuc2100, bacillus thuringiensis knuc2103, and stenotrophomonas maltophilia knuc2106, derived from the korean islands, dokdo and their application on mortar.crack remediation on the surface of cement mortar using microbiological calcium carbonate (caco3) precipitation (micp) has been investigated as a microbial sealing agent on construction materials. however, micp research has never acknowledged the antifungal properties of calcite-forming bacteria (cfb). since fungal colonization on concrete surfaces can trigger biodeterioration processes, fungi on concrete buildings have to be prevented. therefore, to develop a microbial sealing agent that has an ...201323727794
an efficient novel glycosylation of flavonoid by β-fructosidase resistant to hydrophilic organic solvents.an effective approach was successfully developed to isolate glycosidase with resistance of hydrophilic organic solvent, simultaneously with acceptor specificity of the target substrate. by this approach, an efficient solvent tolerant glycosidase producing bacterium arthrobacter nicotianae xm6 was obtained. the β-fructosidase from strain xm6 shows high activity and stability in 10-25% dmso and 10-20% methanol with 90-99% yields of puerarin glycosides. the addition of hydrophilic solvents not only ...201323298773
[immobilization of a recombinant strain producing glucose isomerase on sio2-xerogel and properties of prepared biocatalysts].an original method of immobilization of nongrowing microorganism cells on xerogel of silicon dioxide containing insoluble hydroxyl compounds of cobalt(iii) has been developed. a recombinant strain producing glucose isomerase has been constructed on the basis of escherichia coli with the use of a gene of arthrobacter nicotianae. it was revealed that glucose isomerase activity and stability of biocatalysts prepared on the basis of the recombinant e. coli strain was 3-5 times greater compared with ...201322808740
microbial biodiversity in cheese consortia and comparative listeria growth on surfaces of uncooked pressed cheeses.the study set out to determine how changes in the microbial diversity of a complex antilisterial consortium from the surface of st-nectaire cheese modify its antilisterial activities. on the basis of the microbial composition of a natural complex consortium named tr15 (truefood consortium 15), three new consortia of different species and strain compositions were defined: tr15-sc (58 isolates from tr15 collection), tr15-m (pools of isolates from selective counting media) and tr15-bhi (pools of is ...201424463156
a novel serine hydroxymethyltransferase from arthrobacter nicotianae: characterization and improving catalytic efficiency by rational design.serine hydroxymethyltransferase (shmt) is the key enzyme in l-serine enzymatic production, suggesting the importance of obtaining a shmt with high activity.201425394480
screening micro-organisms for cadmium absorption from aqueous solution and cadmium absorption properties of arthrobacter nicotianae.to obtain basic information on how microbial cells absorb cadmium from aqueous solution, we examined cadmium absorption in various micro-organisms. of 51 micro-organism strains tested, we found that some gram-positive bacteria, such as, arthrobacter nicotianae and bacillus subtilis, and some actinomycetes, such as, streptomyces flavoviridis and s. levoris were highly capable of absorbing cadmium from an aqueous solution. a. nicotianae absorbed the largest amount of cadmium, over 800 μmol cadmium ...201425273147
high-level production of creatine amidinohydrolase from arthrobacter nicotianae 23710 in escherichia coli.in the present study, the gene encoding creatinase was amplified from arthrobacter nicotianae 23710 (cicc) and functionally overexpressed in escherichia coli. by applying a two-stage temperature control strategy, the production of creatinase was increased up to 61.3 u/ml in 3-l fermentor with a high productivity of 6.1 u/ml/h. the recombinant creatinase shows excellent resistance to the chelating agent edta, the surfactants (tween 20, tween 80, and triton x-100) and the common preservative nan3 ...201525536878
effect of volatile organic compounds from bacteria on nematodes.the five studied bacterial strains could produce volatile organic compounds (vocs) that kill nematodes. based on their 16s rrna sequences, these strains were identified as pseudochrobactrum saccharolyticum, wautersiella falsenii, proteus hauseri, arthrobacter nicotianae, and achromobacter xylosoxidans. the bacterial vocs were extracted using solid-phase micro-extraction (spme) and subsequently identified by gc/ms analysis. the vocs covered a wide range of aldehydes, ketones, alkyls, alcohols, al ...201526363885
prospecting cold deserts of north western himalayas for microbial diversity and plant growth promoting attributes.microbial communities in different samples collected from cold deserts of north western himalayas, india, were analyzed using 16s rrna gene sequencing and phospholipid fatty acids (plfa) analysis. a total of 232 bacterial isolates were characterized employing 16s rdna-amplified ribosomal dna restriction analysis with the three restriction endonucleases alu i, msp i and hae iii, which led to formation of 29-54 groups for the different sites, adding up to169 groups. 16s rrna gene based phylogeneti ...201525575970
isolation, development and identification of salt-tolerant bacterial consortium from crude-oil-contaminated soil for degradation of di-azo dye reactive blue 220.the objective of this study was development and characterization of a halophilic bacterial consortium for rapid decolorization and degradation of a wide range of dyes and their mixtures. the 16s rrna gene analysis of developed halophilic consortium vn.1 showed that the bacterial consortium contained six bacterial strains, which were identified as pseudomonas fluorescens hm480360, enterobacter aerogenes hm480361, shewanella sp. hm589853, arthrobacter nicotianae hm480363, bacillus beijingensis hm4 ...201526177415
production of novel nan3-resistant creatine amidinohydrolase in recombinant escherichia coli.creatinase (creatine amidinohydrolase), an important medical enzyme, has been used for clinical diagnosis of renal function because of its high substrate specificity. recently, we successfully cloned a nan3-resistant creatinase encoding gene from arthrobacter nicotianae. by optimizing the cultivation process, we realized its high-level expression in escherichia coli. in this addendum, production of this nan3-resistant creatinase in e. coli and future research were further discussed.201526083084
biodegradation of pentachloronitrobenzene by arthrobacter nicotianae dh19.a pentachloronitrobenzene (pcnb)-degrading bacterial strain was identified as arthrobacter nicotianae based on morphological, physiological and biochemical tests, and 16s rrna gene sequences analysis. the strain could grow on mineral salt medium (msm) containing pcnb as the sole carbon source. under optimal conditions (ph 6·85, 30°c and inoculum biomass amount of 1·45 g l(-1) ), more than 90% residues of pcnb were degraded by strain dh19 within 7 days. strain dh19 could efficiently degrade dichl ...201526250405
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