Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| rapid method for the detection of storage mites in cereals: feasibility of an elisa based approach. | this paper describes the development of rapid immunodiagnostic tests for the detection of storage mite infestations in cereals and cereal products. the study's first phase (proof of concept) involved the production of a species-specific enzyme-linked immunoassay (elisa) for the flour mite, acarus siro (l.), a major pest of stored commodities. the specificity of this new assay was assessed against key stored product contaminants (13 species of mites of which three were predatory, five species of ... | 0 | 18279566 |
| the allergenic importance of house dust and storage mites in asthmatics in brunei, s.e. asia. | examination of dust from sleeping areas showed that storage mites, especially glycyphagid species, were predominant in brunei house dust. on skin testing sixty asthmatics with 1% extracts of six mite species, dermatophagoides pteronyssinus was found to provoke the greatest number of positive skin reactions (66.7%), but positive reactions for tyrophagus putrescentiae (50%), acarus siro (35%), glycyphagus domesticus (40%), and lepidoglyphus destructor (45%) demonstrated that storage mites are also ... | 1980 | 7438419 |
| mites living in hay: an important allergen source? | fifty-two farmers and 21 non-farmers with symptoms related to hay contact were investigated. skin prick tests (spt) and rast were performed with an extract made of barn dust (bde) consisting of hay remnants. at microscopy, the barn dust was found to contain large amounts of dead mites, most of them identified as lepidoglyphus destructor and acarus siro. twenty-one patients (9 farmers and 12 non-farmers) had positive rast or bde, with a good correlation to the case histories. other allergies, esp ... | 1982 | 6897499 |
| occurrence of storage mites in finnish farming environments. | qualitative and semi-quantitative microscopic analyses were performed on the mite populations in dust samples collected from the byres and hay and grain storages of 11 farms in eastern finland. storage mites were found in all farms. the mite fauna was more abundant in the byres and hay storages than in the grain storages. the most common species or genera were acarus siro, acarus farris, glycyphagus domesticus, lepidoglyphus destructor, cheyletus eruditus, tydeus spp., and tarsonemus spp. the re ... | 1982 | 7137517 |
| sensitization in a grain handler to the storage mite lepidoglyphus destructor (schrank). | allergy to one of the most common canadian storage mites. lepidoglyphus destructor, was demonstrated in a grain worker by immediate skin testing and inhalation challenge. the frequency of sensitization to this mite was found to be 12% in 100 asthmatics by skin testing. in 4% of these asthmatics sensitization to another storage mite, acarus siro, was found, all of whom were also sensitized to l. destructor and the house dust mite, dermatophagoides farinae. there was a significantly higher proport ... | 1983 | 6849517 |
| [acari of food storage facilities. an ecologic and immuno-allergic study]. | the authors studied the commonest species of storage mites, living in granaries and in house dust. they found nine species of mites belonging to different systematic groups and were identified: acarus siro, tyrophagus putrescentiae, glycyphagus domesticus, lepidoglyphus destructor, chortoglyphus arcuatus, androlaelaps casalis, cheyletus fortis, cheyletus tenuipilis, thyreophagus entomophagus. skin tests were performed on 14 patients with house dust allergy, using extracts of three storage mites, ... | 1985 | 3837918 |
| allergy to storage mites or cow dander as a cause of rhinitis among finnish dairy farmers. | the role of storage mites acarus siro, lepidoglyphus destructor, and tyrophagus putrescentiae, compared with that of cow dander, as a cause of allergic rhinitis was studied by nasal challenges in a population of 106 non-smoking dairy farmers. thirty-six of the subjects were challenged with storage mites alone, each with only one of the three species in consecutive order, 43 with cow dander alone, and 27 with both. thus mite challenges were performed on 63 farmers and cow dander challenges on 70. ... | 1985 | 3977027 |
| storage mites as a cause of bronchial asthma in denmark. | from october 1982 to july 1983, 133 consecutive patients complaining of allergic symptoms were prick-tested with three storage mites (acarus siro, lepidoglyphus destructor, tyrophagus putrescentiae) and two housedust mites (dermatophagoides pteronyssinus and dermatophagoides farinae). a total of 60 patients (45%) had a positive prick-test for one or more of the storage mites. a mutual positive correlation was found between the prick-test results for the two house-dust mites as well as for the th ... | 1985 | 4003230 |
| storage mite allergy is common in a farming population. | the prevalence of storage mite allergy was investigated in an epidemiological study of respiratory symptoms in farmers on gotland, an island in the baltic sea. a questionnaire concerning work-related and chronic symptoms from the airways and eyes was completed by 2578 farmers. a sample of 440 farmers subsequently underwent examination comprising skin-prick tests (animal danders, pollens, moulds and house dust mite), blood sampling for rast against four storage mites (acarus siro, lepidoglyphus d ... | 1985 | 4075515 |
| [allergy to tyrophagus putrescentiae and lepidoglyphus destructor in a population of young adult asthmatics]. | the goal of this work was the study of the prevalence of allergy to two mites, tyrophagus putrescentiae and lepidoglyphus destructor in a population of 105 young asthmatic men from east of france. the association of skin tests and specific ige shows that 43.10% are allergic to tyrophagus and 44.95% to lepidoglyphus destructor. the significance of skin tests is doubtful and depends on the laboratories which provide the allergens. therefore, specific ige has to be performed. as in other studies it ... | 1986 | 3453717 |
| storage mites in the work environment of farmers. | occurrence of storage mites was studied in cow houses and hay stores on 19 farms, most of which were located in eastern finland. on two farms we did a monthly follow-up. mites were extracted from hay by sieving, using the so-called "water trap" and for longitudinal analysis, with a warm extractor. the mites isolated in the water trap were identified under a microscope. contrary to previous finnish findings, there apparently were more mites in cow houses than in hay stores. in cow houses there we ... | 1987 | 3478210 |
| sensitization to storage mites and other work-related and common allergens among finnish dairy farmers. | skin-tests with the prick technique were made on 121 dairy farmers with allergic rhinitis and/or asthma (symptomatic subjects), 64 dairy farmers without respiratory symptoms (asymptomatic subjects), and 26 non-farming controls. the antigen panel consisted of the storage mites acarus siro, lepidoglyphus destructor, and tyrophagus putrescentiae, as well as other work-related allergens (cereal grains, animal epithelia, fungi and yeasts), and common allergens (house dust mite, pollens). mean areas o ... | 1987 | 3478215 |
| predominance of mite allergy over allergy to pollens and animal danders in a farming population. | the prevalence of ige-mediated hypersensitivity to respiratory allergens, including lepidoglyphus destructor (a storage mite) and dermatophagoides pteronyssinus, was studied in a rural population of gotland, a swedish island in the baltic sea. a sample of 440 farmers underwent examination, comprising skin-prick tests (animal danders, pollens, moulds, house dust mite and wheat) and blood sampling for radio-allergosorbent tests (rast) (birch, timothy grass, dog, d. pteronyssinus, cladosporium and ... | 1987 | 3499998 |
| storage mite allergy among grain elevator workers. | a total of 139 workers (71% of the available work force) in four grain elevator stores in aalborg, denmark, participated in a cross-sectional study on the prevalence of storage mite allergy among grain elevator workers. all underwent interview, prick test with a standard panel of allergens and the storage mites acarus siro, lepidoglyphus destructor and tyrophagus putrescentiae, rast to the three above-mentioned storage mites plus glycyphagus domesticus and wheat, measurement of serum total ige, ... | 1987 | 3661920 |
| lack of allergenic cross-reactivity between storage mites and dermatophagoides pteronyssinus. | the allergenic cross-reactivity between storage mites (lepidoglyphus destructor, glycyphagus domesticus, tyrophagus putrescentiae and acarus siro) and the house dust mite dermatophagoides pteronyssinus was studied with the radio-allergo-sorbent test (rast) and the rast inhibition technique. rast-positive sera were obtained from fifty-three farmers who were positive to at least one of the four storage mites. sera from twelve of these farmers, eight of whom were also positive to d. pteronyssinus, ... | 1987 | 3829368 |
| [allergy to thyrophagus putrescentiae and lepidoglyphus destructor in a population of young asthmatic adults]. | in the present work a study has been made of the prevalence of allergy to two storage mites, tyrophagus putrescentiae and lepidoglyphus destructor in a population of 105 young adults in the east of france. combination of skin tests and specific ige measurements shows that 43.10% of the subjects were sensitive to tyrophagus and 44.95% to lepidoglyphus. interpretation of skin tests remains uncertain, particularly concerning the source of the allergen used and it is most often also necessary to mea ... | 1987 | 3453125 |
| genetic variability and ecological adaptability of hypopus formation in a stored product mite. | occurrence in lepidoglyphus destructor of a facultative developmental stage (hypopus) adapted for dispersal and dormancy depends on genotype x environment interaction. dietary factors affect hypopus formation, but the response of individuals to food quality greatly differs according to genotype. large genetic variation in the control of hypopus formation exists within as well as between populations. response to selection is rapid and reversible. the trait has substantial potential for selective ... | 1987 | 3453331 |
| bronchial provocation studies in farmers with positive rast to the storage mite lepidoglyphus destructor. | bronchial provocation studies with l. destructor (l. des.) extract were made on 12 farmers with asthma who were exposed daily to stored hay and grain and had positive radio-allergo-sorbent test (rast) to l. des. all 12 farmers developed more than a 20% fall in fev1 to the extract. none of the symptomatic farmers who were also rast positive to the l. des. culture medium developed an asthmatic reaction to the medium extract. neither four healthy farmers nor two patients with asthma caused by d. pt ... | 1988 | 3207179 |
| demonstration of allergen components in the storage mite lepidoglyphus destructor by an immunoblotting technique. | the allergen composition of crude extracts of the storage mite lepidoglyphus destructor were investigated by sodium dodecylsulphate polyacrylamide gel electrophoresis and immunoblotting. the allergen components were detected by sera from allergic farmers with a positive radioallergosorbent test to l. destructor. the allergen-antibody complexes were visualized by rabbit anti-ige and beta-galactosidase-labelled sheep antirabbit igg in a chromogenic substrate. six out of the forty-three sera also h ... | 1988 | 3338856 |
| [pathology caused by acari]. | this report concerns the observations the authors have been carrying out for over twelve years in sardinia; they regard the sensitization to house dust mites (dermatophagoides pteronyssinus, dermatophagoides farinae) and to storage mites (acarus siro, glycyphagus domesticus, lepidoglyphus destructor, tyrophagus putrescentiae) and are been obtained with: 1) skin tests with mite extracts; 2) radioallergosorbenttest (rast) for specific ige and igg; 3) mite exposure in homes and mite analysis; 4) hy ... | 1989 | 2519754 |
| skin and serum reactivity to some storage mites in children sensitive to dermatophagoides pteronyssinus. | the incidence of skin test and rast positivity for dermatophagoides farinae and storage mites (glycyphagus privatus, lepidoglyphus destructor, tyrophagus putrescentiae, acarus siro, and gohieria fusca) have been evaluated in children with bronchial asthma and positive prick test to dermatophagoides pteronyssinus. the storage mite with highest incidence of skin and rast positivity was gohieria fusca, which caused a positive response in more than 50% of the children evaluated. this antigen appears ... | 1989 | 2742217 |
| aeroallergens in dairy barns near cooperstown, new york and rochester, minnesota. | we sampled atmospheric barn air using a volumetric air sampler in ten barns near cooperstown, ny and six barns near rochester, mn, and, with radioimmunoassays, measured allergens of aspergillus fumigatus, thermoactinomyces vulgaris, micropolyspori faeni, short ragweed, rye grass group i pollen, alternaria (alt-1), dermatophagoides sp. lepidoglyphus destructor, common insect allergen, mouse urine, rat urine, and cattle epithelium. the most abundant allergen present was a. fumigatus followed by l. ... | 1989 | 2764367 |
| clinical significance and allergenic cross-reactivity of euroglyphus maynei and other nonpyroglyphid and pyroglyphid mites. | the clinical significance and allergenic cross-reactivity of the storage mites lepidoglyphus destructor and acarus siro and the house dust mites dermatophagoides pteronyssinus and euroglyphus maynei were investigated with specific ige antibodies by use of the rast and the rast-inhibition technique. rast-positive sera were obtained from 64 swedish farmers whose test results were positive to at least one of the four mite species. e. maynei was shown to be a common cause of sensitization among the ... | 1989 | 2926080 |
| prevalence of specific ige to storage mites (a. siro, l. destructor and t. longior) in an urban population and crossreactivity with the house dust mite (d. pteronyssinus). | the role of pyroglyphid mites in house dust allergy is well established and the major allergens from the common house dust mites (dermatophagoides species) have been characterized. there is, however, relatively little progress in the understanding of the human ige response to non-pyroglyphid storage mites, allergenic crossreactivity with other mite species and extent of environmental exposure. we studied 196 individuals from an urban environment who were not occupationally exposed to storage mit ... | 1990 | 1695869 |
| comparison of clinical significance and allergenic cross-reactivity of storage mites blomia kulagini and lepidoglyphus destructor in sweden and brazil. | comparison of the clinical significance and allergenic cross-reactivity of blomia kulagini (b. kulagini) and lepidoglyphus destructor (l. destructor) was made on sera from sweden and brazil using the radio-allergo-sorbent test (rast) and the rast inhibition technique. rast-positive sera were obtained from 53 allergic swedish farmers and 31 allergic subjects from brazil who were positive to b. kulagini and/or l. destructor. b. kulagini was shown to be a common cause of sensitization especially in ... | 1990 | 2244671 |
| dermatophagoides and storage mites: statistical analysis of rast results. | r.i.a. dosages of specific ige antibodies to dermatophagoides pteronyssinus, dermatophagoides farinae (house dust mites), acarus siro, lepidoglyphus destructor, and tyrophagus putrescentiae (storage mites) in 467 unselected patients were statistically analyzed. a significant noncorrelation between specific ige antibodies to storage mites and those to house dust mites has been found, suggesting that storage mites have their own allergenic specificity. | 1990 | 2321810 |
| storage mite allergy among bakers. | allergy to storage mites has been shown among farmers and grain elevator workers. this study aimed to examine whether bakers also are sensitized to storage mites. twenty-three bakers or other employees in bakeries suffering from nasal or pulmonary symptoms were compared with 17 control persons with no relation to bakery work or agriculture. all participants underwent skin prick test with a standard panel of allergens, four types of flour and the storage mites acarus siro, lepidoglyphus destructo ... | 1990 | 2327560 |
| [sensitization against storage mites in allergic rhinopathy]. | in the past 15 years, storage mites became more common as an agent in allergic airway disease. sensitization to storage mites has been reported in farmers and grain workers. there were some studies investigating local populations, especially in the north of europe. the sensitization to storage mites in a rural population seems to be similar to the rate found for dermatophagoides. the main diseases caused by storage mites are allergic rhinitis and asthma. although there is a cross-reactivity betw ... | 1991 | 1755912 |
| [sensitization to granary mites in patients with allergic diseases]. | as many as 71 patients selected from a group of 550 patients suffering from allergic diseases, sensitized to the house dust mites dermatophagoides pteronyssinus were examined. a study was made of the presence of allergen-specific ige antibodies against house dust mites, storage mites (7 species altogether) using allergenic discs and commercial kits rast (pharmacia, sweden). the group under examination mostly manifested sensitization to the house dust mites: dermatophagoides ferinae (80%), eurogl ... | 1991 | 2063323 |
| skin cross-reactivity between dermatophagoides pteronyssinus and storage mites in atopic children. | in our environment, some children with allergic respiratory pathology do not have as favorable an evolution as expected after three years of immunotherapy with d. pteronyssinus extracts. we believe this is due to the existence of other mites whose antigenic determinants are not present in d. pteronyssinus. the purpose of the present study was to evaluate skin cross-reactivity between dermatophagoides and storage mites in children with bronchial asthma. fifty patients were chosen, with a mean age ... | 1991 | 1669575 |
| sensitization to blomia tropicalis and lepidoglyphus destructor in dermatophagoides spp-allergic individuals. | serum was collected from 77 atopic subjects from cartagena, colombia, who had a positive skin test (greater than or equal to 3 mm) to either dermatophagoides farinae (df) (73) or d. pteronyssinus (dp) (70). twenty-nine healthy volunteers with negative allergy histories and negative skin tests were used as control subjects. rast and rast-inhibition assays were used to study the prevalence of specific ige to blomia tropicalis (bt), lepidoglyphus destructor (ld), and df. rast results were considere ... | 1991 | 1744365 |
| storage mites dominate the fauna in swedish barn dust. | the mite fauna in hay dust of 16 farms located on the island of gotland was analysed to establish whether there is a clear relationship between environmental exposure to storage mites and development of allergic symptoms. eight of these farms belonged to farmers with respiratory symptoms related to barn dust (rs) and eight to healthy farmers (h). all farmers had previously participated in an epidemiological survey of hypersensitivity to respiratory allergens. the symptomatic farmers had not only ... | 1991 | 2039080 |
| late asthma caused by inhalation of lepidoglyphus destructor. | we report the case of a grain worker who had immediate nasal and conjunctival symptoms and late-phase asthmatic reactions after handling stored grain. allergy to one storage mite, lepidoglyphus destructor, was demonstrated by skin testing, specific ige, and bronchial provocation tests. bronchial challenge with l. destructor provoked only a late phase asthmatic reaction without an immediate reaction despite the fact that immediate skin tests and specific ige had been positive. pretreatment with c ... | 1991 | 1867448 |
| identification of ige-binding proteins from lepidoglyphus destructor and production of monoclonal antibodies to a major allergen. | the allergen composition of one of the most important storage mites, lepidoglyphus destructor, has been studied by immunodetection after sds-page with individual patient sera. an allergenic polypeptide of 14 kda was identified with 95% of the sera. this major allergen was isolated in the supernatant of 60% ammonium sulfate salt precipitation of the whole extract, which was subsequently used to immunize balb/c mice so as to produce monoclonal antibodies. four mabs recognizing molecules with ige-b ... | 1991 | 1722776 |
| genetic and environmental determinants of hypopus duration in the stored-product mite lepidoglyphus destructor. | this paper reports a series of experiments over many years on hypopus duration and extends the preceding investigation (1987) on hypopus formation in lepidoglyphus destructor (schrank, 1781). the length of time required for hypopus physiogenesis (diapause development) is genetically programmed but influenced by environmental factors. this span of time is highly variable, and may extend from one week to more than a year. spreading out the potential for hypopus completion over time is adaptive, si ... | 1991 | 2044432 |
| identification of a new major allergen of 39 kilodaltons of the storage mite lepidoglyphus destructor. | the allergen composition of a non-denatured extract of the storage mite lepidoglyphus destructor was studied by a combination of hybridoma technology, sodium dodecylsulphate polyacrylamide gel electrophoresis (sds-page) and a sandwich radio-allergosorbent test (four-step rast). using hybridoma technology, monoclonal antibodies (mabs) were generated against the non denatured extract of the l. destructor. after screening by elisa, mab 42b6 was selected for further studies. this mab specifically re ... | 1991 | 2026455 |
| immunoblot multi-allergen inhibition studies of allergenic cross-reactivity of the dust mites lepidoglyphus destructor and dermatophagoides pteronyssinus. | the allergenic similarity of the pyroglyphid mite d. pteronyssinus and the glycyphagid mite l. destructor was investigated with a new immunoblotting inhibition technique allowing simultaneous comparison of several allergens. extracts of d. pteronyssinus and l. destructor were separated by sds-page and electroblotted to nitrocellulose (nc). a serum pool containing ige specific to the major allergens in both mites was mixed with serially diluted extracts of d. pteronyssinus and l. destructor and i ... | 1991 | 1913274 |
| n-terminal aminoacid sequence of principal allergen of storage mite lepidoglyphus destructor. | 1992 | 1355192 | |
| sensitization to the storage mite lepidoglyphus destructor in wheat flour respiratory allergy. | occupational allergy due to hypersensitivity to cereal flours is relatively common among bakers and grain-store workers. storage mites can contaminate wheat flour and could be an important cause of allergic symptoms due to inhalation. forty-three patients with criteria for allergic sensitization to wheat flour (skin tests, specific ige to wheat flour and positive challenge tests) were included in a study to investigate the prevalence of cosensitization to lepidoglyphus destructor (ld). this mite ... | 1992 | 1586004 |
| purification and characterization of lep d i, a major allergen from the mite lepidoglyphus destructor. | a major allergen of the storage mite lepidoglyphus destructor (lep d i) has been purified by affinity chromatography using an anti-lep d i monoclonal antibody. the purity of the protein obtained by this procedure was assessed by reverse-phase hplc. lep d i displayed a molecular weight of 14 kd on sds-page under non-reducing conditions, and 16 kd in the presence of a reducing agent. analytical ief revealed a little charge microheterogeneity, showing three bands with pis 7.6-7.8. purified lep d i ... | 1992 | 1611545 |
| localization of allergens in the domestic mite lepidoglyphus destructor. | the non-pyroglyphid domestic mite lepidoglyphus destructor is a major source of allergen causing respiratory symptoms in farming environments. this study is the first to focus on the localization of the allergens in the non-pyroglyphid domestic mite lepidoglyphus destructor. cryostat-cut sections of l. destructor mite bodies and faecal pellets were probed with one of three mouse monoclonal antibodies (moabs) raised against l. destructor or with patient sera, and stained with immunoperoxidase. ei ... | 1992 | 1373986 |
| comparison of allergic responses to dust mites in u.k. bakery workers and swedish farmers. | the ige rast response to dermatophagoides pteronyssinus and four storage mites (lepidoglyphus destructor, tyrophagus putrescentiae, glycyphaghus domesticus, and acarus siro) was examined in 251 u.k. bakery workers and compared with that previously found in 440 swedish farmers. storage mites are found commonly in stored hay and grain so both these groups potentially encounter them in their work. in neither group of workers was a positive rast (greater than or equal to 0.35 pru) to d. pteronyssinu ... | 1992 | 1571817 |
| monoclonal antibodies to lepidoglyphus destructor: delineation of crossreactivity between storage mites and house dust mites. | we have developed monoclonal antibodies (moabs) to the storage mite lepidoglyphus destructor (ld). employing these anti-ld moabs ld-moabs) in elisa and elisa inhibition techniques we have analysed the reaction pattern of ld-moabs to both non-pyroglyphid and pyroglyphid mites. the storage mite glycyphagus domesticus (gd) exhibited most efficient inhibition, followed by acarus siro (as), tyrophagus putrescentiae (tp), dermatophagoides pteronyssinus (dpt) and euroglyphus maynei (em). of the two pyr ... | 1992 | 1468029 |
| study of the sensitization to storage mites in a pediatric population in barcelona. | we performed the following study in order to determine the prevalence and characteristics of sensitization to storage mites in children mostly from the city of barcelona. all children over 3 years of age attending our unit for the first time due to respiratory problems were given cutaneous tests using the prick test for the house dust mites dermatophagoides pteronyssinus and d. farinae, and the storage mites acarus siro, lepidoglyphus destructor and tyrophagus putrescentiae. we carried out a ras ... | 1993 | 8281346 |
| mite allergy in the tropics: sensitization to six domestic mite species in cartagena, colombia. | sensitization to the domestic mites blomia tropicalis, chortoglyphus arcuatus, lepidoglyphus destructor and aleuroglyphus ovatus was investigated in 77 subjects with clinical symptoms of asthma and/or allergic rhinitis and a positive skin prick test (wheal > or = 3 mm) to dermatophagoides pteronyssinus and/or d. farinae. seventy patients were skin test-positive to d. pteronyssinus and 73 to d. farinae. serum samples from 29 nonallergic, skin test-negative individuals were used as negative contro ... | 1993 | 8281353 |
| inhaled aeroallergen and storage mite reactivity in a wisconsin farmer nested case-control study. | a nested case-control study was undertaken to assess the role of antibody (ab) to a panel of antigens associated with hypersensitivity pneumonitis in the development of lung disease among wisconsin dairy farmers. immunologic reactivity to a panel of inhaled allergens was assessed in the ab+ cases and compared to the ab+ control, ab- control, and ab- blue-collar control subjects by skin tests, rast assays, and a modified american thoracic society questionnaire. the most prevalent allergens among ... | 1993 | 8430958 |
| occurrence of ige antibodies against the 39-kda allergen component of the mite lepidoglyphus destructor in urban and rural subjects. | several species of storage mite have been shown to cause ige-mediated sensitization. monoclonal antibodies (mabs) have been raised against the storage mite lepidoglyphus destructor, and one mab (42b6) immunoprecipitated a band of 39 kda of l. destructor. to search for the frequency of ige antibodies in patient sera against this allergen component, we modified a radioimmunoassay (ria), 42b6 being used as capturing antibody. twenty of 43 sera from farmers who were rast positive to l. destructor (4 ... | 1993 | 8506991 |
| sensitization to storage mites in house dust mite (dermatophagoides pteronyssinus) allergic patients. comparison of a rural and an urban population. | in this study, sera collected from 50 patients (24 females, 26 males) with type i allergy to house dust mite (dermatophagoides pteronyssinus) were investigated for ige antibodies specific for eight different mite species including storage mites of the families pyroglyphidae, glycyphagidae and acaridae. according to their environment the patients were divided into two groups. group i consisted of 24 (11 women, 13 men) farmers working and living in rural regions of austria (styria, lower austria), ... | 1994 | 8039020 |
| primary structure of lep d i, the main lepidoglyphus destructor allergen. | the most relevant allergen of the storage mite lepidoglyphus destructor (lep d i) has been characterized. lep d i is a monomer protein of 13273 da. the primary structure of lep d i was determined by n-terminal edman degradation and partially confirmed by cdna sequencing. sequence polymorphism was observed at six positions, with non-conservative substitutions in three of them. no potential n-glycosylation site was revealed by peptide sequencing. the 125-residue sequence of lep d i shows approxima ... | 1994 | 7925475 |
| dust mite allergy: an important cause of respiratory disease in farmers. | in 2,578 swedish farmers, 6.2% were found to have an allergy to storage mites. the storage mite lepidoglyphus destructor has been identified by both rast and bronchial challenges as a significant cause of occupational asthma in rural environments. immunohistochemical studies of l. destructor disclosed that at least one l. destructor allergen is associated with digestion. | 1994 | 8116651 |
| allergenic characterization of acarus siro and tyrophagus putrescentiae and their crossreactivity with lepidoglyphus destructor and dermatophagoides pteronyssinus. | allergenic components in extracts of acarus siro and tyrophagus putrescentiae were identified by sodium dodecyl sulphate-polyacrylamide (sds-page) and immunoblotting. five and four allergenic components, respectively, were visualized with sera from farmers sensitized to storage mites. the highest frequency of ige-binding was to a 15 kda component of a. siro (7/9 sera) and a 16 kda component of t. putrescentiae (23/29 sera). the allergenic crossreactivity of a. siro and t. putrescentiae with lepi ... | 1994 | 7982124 |
| detection of at least one high-molecular-mass, ige-binding component of the dust mite lepidoglyphus destructor. | we studied the allergen composition in an extract of the storage mite lepidoglyphus destructor. monoclonal antibodies (mabs) were raised against l. destructor by a standard hybridoma technique. in the subsequent screening, we concentrated on mabs fulfilling three criteria. first, in elisa, mabs were assessed against a panel of various mite species, and only those reacting exclusively with l. destructor extract were selected for further analyses. secondly, mabs were selected in immunoblotting acc ... | 1994 | 7653740 |
| a naturally occurring model of immunoglobulin e antibody-mediated hypersensitivity in laboratory animals. | a naturally occurring model of immunoglobulin e (ige) antibody-mediated hypersensitivity in canine and rhesus monkey species to environmental allergens is described. cutaneous responses to a dust mite mix (dermatophagoides farinae/pteronyssinus) and to four storage mite extracts (aleuroglyphus ovatus, blomia tropicalis, chortoglyphus sp., lepidoglyphus destructor) occurred in the canine species. rhesus monkeys had ige antibody-mediated hypersensitivity to dust mite on cutaneous challenge, but to ... | 1994 | 8201269 |
| [atopic allergy in an icelandic urban population 20-44 years of age.] | this is a part of a second stage of ec respiratory health survey and the aim of this study is to estimate the prevalence of atopic allergy among urban population of men and women aged 2cm4 years, living in the capital reykjavik and suburbs. altogether 800 individuals were invited of whom 570 participated (77%). all participants answered questionnaire and 540 individuals were skin prick tested by 12 allergens. 20.5% had positive skin tests, defined as at least one allergen positive by a weal reac ... | 1995 | 20065461 |
| crossreactivity between dermatophagoides siboney and other house dust mite allergens in sensitized asthmatic patients. | the role of pyroglyphid mites in ige-mediated sensitization has been well established. although mites belonging to the genus dermatophagoides dominate in the acarofauna of domestic dust, non-pyroglyphid mites might also be of clinical importance. in cuba, dermatophagoides siboney is found in dust coexisting with d. pteronyssinus and blomia tropicalis. dermatophagoides farinae is not found. storage mites, such as acarus siro, lepidoglyphus destructor and tyrophagus putrescentiae, might also be pr ... | 1995 | 8556563 |
| [skin tests for different species of acari in asthmatic children from mexico city]. | house dust mites, dermatophagoides pteronyssinus (dp) and dermatophagoides farinae (df) are the predominant cause of allergic asthma in our population. to detect the presence of sensitivity to other mites besides dp and df in 100 children that live in mexico city, skin tests were performed in their arms with multi-test devices and glycerinated extracts. there were 34 female and 66 male, aged 4 to 14 years (x = 9.1y), with history of mild and moderate asthma, 2 months to 12 years of evolution. dp ... | 1995 | 7552183 |
| food-induced and occupational asthma due to barley flour. | occupational exposure to inhalant allergens may induce asthma but the presence of asthma after the ingestion of the allergen is rarely reported. | 1995 | 7648375 |
| localization of major allergens in the dust mite lepidoglyphus destructor with confocal laser scanning microscopy. | the dust mite lepidoglyphus destructor is the dominating source of allergens giving rise to asthma and rhinitis among farmers. in a previous study of the localization of allergens in l. destructor we demonstrated that the 39 kda allergen is associated with digestion. here we describe the localization of the principal 15 kda allergen and the high molecular weight allergen complex (79 and 93 kda) in l. destructor with confocal laser scanning microscopy (clsm). cryostat-cut sections of mite bodies ... | 1995 | 7648460 |
| cdna analysis of the mite allergen lep d 1 identifies two different isoallergens and variants. | for the first time the complete cdna encoding two isoallergens of the non-pyroglyphid dust mite lepidoglyphus destructor, lep d 1, allergen has been sequenced. in addition, one of the isoallergens was found to have two variants. oligonucleotides were designed from known amino acid sequences. the cdna sequences were obtained by hybridizing these primers to mrna and enhancement by the rt-pcr technique. to obtain the different complete encoding cdna sequences and eliminate heteroduplex artifacts, w ... | 1995 | 7649288 |
| igg1 and igg4 antibody responses to the dust mite lepidoglyphus destructor in a naturally exposed farming population. | the natural humoral immune response to the dust mite lepidoglyphus destructor was assessed by comparing the igg1 and igg4 responses elicited in allergic (n = 44) and healthy (n = 16) individuals in a farming population chronically exposed to this allergen. with the aid of an immunoblotting technique and elisa, the sera were analyzed for anti-l. destructor antibodies. while the majority of sera from the allergy group displayed several bands for both igg1 and igg4, the nonatopic healthy group was ... | 1995 | 7573839 |
| interaction of allergens from house-dust mite and from cereal flours: dermatophagoides pteronyssinus alpha-amylase (der p 4) and wheat and rye alpha-amylase inhibitors. | major wheat and rye allergens associated with baker's asthma have been identified as specific inhibitors of dermatophagoides pteronyssinus alpha-amylase (der p 4). the most active inhibitors were the wheat tetrameric one and the rye dimeric rdai-1. these data suggest that interaction between mite and cereal allergens (alpha-amylase/inhibitor complexes) might occur in house-dust-mite-infected flours. in contrast to the strong inhibition of the d. pteronyssinus enzyme by the wheat and rye inhibito ... | 1996 | 8781672 |
| allergen-specific ige levels and mite allergen exposure in children with acute asthma first seen in an emergency department and in nonasthmatic control subjects. | sensitization to allergens has been shown to be a risk factor for adults with acute asthma first seen in the emergency department. | 1996 | 8757201 |
| sensitization to dermatophagoides siboney, blomia tropicalis, and other domestic mites in asthmatic patients. | mite species adapted to warm, humid climates are commonly found in house dust in the tropics. in cuba, dermatophagoides pteronyssinus, d. siboney, and blomia tropicalis are the most common and abundant mite species in house dust. to investigate the pattern of sensitization of cuban asthmatic patients to common mite species, we skin-prick-tested (spt) 148 patients with a clinical history of asthma and possible mite allergy, and determined specific ige antibodies against mite allergens (d. pterony ... | 1996 | 8863927 |
| mite sensitivity and exposure in the city of santa fe, argentina. | little information is available on mite allergen levels and rates of sensitization to domestic mites in south america. the purpose of this study was to establish the rate of sensitization to domestic mites in asthmatics and measure mite allergen levels in pillows and mattresses of mite allergic individuals in santa fe, argentina. dust samples were collected from the pillows and mattresses of 56 asthmatics (24 females and 32 males, mean age 17.6 +/- 11.5 years), who had been previously skin teste ... | 1996 | 8959537 |
| [new aero-allergens]. | as the number of proteins recognized as causing allergic respiratory diseases increases, new aero allergens have appeared in the animal and vegetable realms, both in home and professional environments. lepidoglyphus destructor and blomia tropicalis, two mites found in storage areas, are particularly important in agricultural areas and in homes. over the last ten years, the frequency of reactions to cockroaches has also increased in several countries. the allergenicity of non-biting insects is a ... | 1996 | 8761637 |
| elisa method for detection of mite allergens in barn dust: comparison with mite counts. | elisa (enzyme-linked immunosorbent assay) inhibition with a monoclonal antibody (mab) (42b6) to lepidoglyphus destructor was used to detect and quantify the storage-mite allergens in 30 dust samples collected from barns. regarding the mite fauna, microscopic inspection of the barn dust and mite counts showed that l. destructor infested all 30 barns investigated (range 430-195 400 mites/g dust). in 29/30 barns, l. destructor constituted more than 70% of the astigmata species. acarus siro was foun ... | 1996 | 8792923 |
| sensitization to lepidoglyphus destructor, tyrophagus putrescentiae, and acarus siro in patients allergic to house dust mites (dermatophagoides spp.). | 1997 | 9389307 | |
| ige antibodies to aeroallergens in allergic children in são paulo, brazil. | the sensitization to inhaled allergens was studied in 80 brazilian children and 12 controls, aged 6 to 16 years. we performed skin tests with alternaria alternata, cat, dog, lolium perenne, grasses and the following domestic mites: blomia tropicalis, dermatophagoides pteronyssinus, dermatophagoides farinae, lepidoglyphus destructor, chortoglyphus arcutus and aleuroglyphus ovatus. the frequencies of positive skin tests (mean diameter > or = 23 mm) were respectively 15%, 11%, 11%, 6%, 7%, 95%, 92% ... | 1997 | 9330189 |
| occupational allergic disease in cereal workers by stored grain pests. | it is well known that workers occupationally exposed to grain dust have a high prevalence of respiratory symptoms, but their pathogenesis remains obscure when sensitization to cereal flour cannot be demonstrated. storage mites, tenebroids, and cockroaches are stored-grain pests found in grain and cereal products frequently in our area, where the cereal industry is the most important industry. an epidemiological analysis of sensitization of these stored-grain pests was performed on 4379 patients ... | 1997 | 9350153 |
| mite fauna of german farms. | storage mites are important sources of airborne allergens, especially on farms. a mite survey was conducted in the working environments of 121 farms in five regions of germany. of 859 samples, 743 (86.4%) contained mites, representing 547,857 astigmatic mites in 723 g of dust and 34,501 prostigmatic mites in 469 g of dust. dust samples from nine sites of each farm were collected and analyzed. ninety-three percent of all mites belonged to the order astigmata (storage- and house-dust-mite species) ... | 1997 | 9450144 |
| house dust mite fauna in turkey. | storage and house dust mites (hdm) appear to be important allergen sources in sensitive patients. the acarofauna of dust samples collected from 133 homes located in five regions of anatolia with different weather conditions were evaluated. hdm and/or storage mites were found in 32 samples. twenty of them were identified as containing d. pteronyssinus and d. farinae, and 21 had storage mites. lepidoglyphus destructor and gamasidae were the most frequently found, followed by cheyletus eraditus, gl ... | 1997 | 9491198 |
| allergenic crossreactivity between lepidoglyphus destructor and blomia tropicalis. | in general, the non-pyroglyphid mites lepidoglyphus destructor and blomia tropicalis show a different geographical distribution. allergic sensitization to both species have been demonstrated in several investigations. however, whether this reflects cross-reactivity or dual sensitization is so far not known. | 1997 | 9208191 |
| recombinant mite allergens. new technologies for the management of patients with asthma. | the introduction of molecular cloning techniques has led to advances in allergen identification and sequencing, production of recombinant allergens, identification of b-cell and t-cell epitopes, and tertiary structural analysis of allergen molecules. over 10 groups of mite allergens have been cloned from dermatophagoides spp., as well as several homologous allergens from euroglyphus maynei, lepidoglyphus destructor, and blomia tropicalis. the availability of these allergens has made it feasible ... | 1997 | 9188916 |
| occurrence of mites in norway and the rest of scandinavia. | this paper gives a faunistic review of mites that have been recorded in the indoor environment, particularly homes, barns, and stored products, in norway and the other scandinavian countries. some preliminary results are given from unpublished investigations in norway. seven species of pyroglyphid mites have been recorded: dermatophagoides pteronyssinus, d. microceras, d. farinae, d. evansi, euroglyphus maynei, gymnoglyphus longior, and hirstia chelidonis. d. pteronyssinus was the predominant sp ... | 1998 | 10096804 |
| sensitization to storage mites in urban working environment. | this paper presents an analysis of a medical history questionnaire, skin prick test and specific ige to storage mites lepidoglyphus destructor and tyrophagus putrescentiae performed on 26 male paper mill workers and 36 postmen. positive prick test were considered urticas with the mean diameter of 3 mm or over. increased specific ige were considered values of over 0.35 ku/l. paper mill workers manifested a significantly higher frequency of positive test results, increased specific ige and positiv ... | 1998 | 9810760 |
| expression of two isoforms of lep d 2, the major allergen of lepidoglyphus destructor, in both prokaryotic and eukaryotic systems. | the dust mite lepidoglyphus destructor is a major cause of allergic diseases among farmers. we have previously cloned and sequenced two isoforms of the major allergen lep d 2 (formerly designated lep d 1) and found significant homology to group 2 allergens of the house dust mite species dermatophagoides. we now report on the production and characterization of recombinant lep d 2. | 1998 | 9756203 |
| ige binding capacity of synthetic and recombinant peptides of the major storage mite (lepidoglyphus destructor) allergen, lep d 2. | lepidoglyphus destructor is an important non-pyroglyphid mite species in europe and a dominant allergen in farming environments. the major allergen of l. destructor, lep d 2, is a protein of 13.2 kd that is recognised by about 90% of sera rast positive to this mite species. | 1998 | 9831803 |
| contribution of disulphide bonds to antigenicity of lep d 2, the major allergen of the dust mite lepidoglyphus destructor. | to study the contribution of the 3 disulphide bonds in the major allergen lep d 2 to the antigenic structure, site-directed mutagenesis was performed. mutants with one or more cysteine residues altered were constructed with a histidine residue tag for purification purposes and expressed as recombinant proteins in e. coli. seven mutants were analysed: 3 single mutants (cys 8, cys 21 and cys 72), 3 double mutants (cys 8-117, cys 21-16 and cys 72 77) and one mutant with all 6 cysteines altered (6 c ... | 1998 | 10068036 |
| cross-reactivity between dermatophagoides siboney and other domestic mites. ii. analysis of individual cross-reacting allergens after sds-page and western blotting inhibition. | dermatophagoides siboney (ds) cross-reacted with other mite allergens in mite-allergic patients. the aim of this study was to investigate the cross-reactivity between individual allergens responsible for this cross-reactivity. | 1998 | 9693268 |
| prevalence of sensitization to the storage mites acarus siro, tyrophagus putrescentiae, and lepidoglyphus destructor in allergic patients with different degrees of sensitization to the house-dust mite dermatophagoides pteronyssinus. | the prevalence of sensitization to the storage mites acarus siro (as), tyrophagus putrescentiae (tp), and lepidoglyphus destructor (ld) was studied in 250 sera of patients with different degrees of sensitization to the house-dust mite dermatophagoides pteronyssinus (dp) by measuring ige binding to extracts of the storage mites. additionally, allergenic cross-reactivity between dp and the storage mite species was studied by rast inhibition with five individual sera (and a pool of these sera) with ... | 1998 | 9574887 |
| clinical and immunologic aspects of storage mite allergy. | exposure to storage (nonpyroglyphid) mites has been increasingly recognized as a cause of asthma and rhinitis. several species have been identified in europe; e.g., lepidoglyphus destructor, acarus farris/siro, tyrophagus spp., glycyphagus domesticus, and blomia tjibodas. blomia tropicalis, on the other hand, predominates in subtropical and tropical areas. studies from several countries have shown that ige-mediated allergy in rural populations is of considerable importance and that storage mites ... | 1998 | 10096808 |
| [airborne immediate allergy in mid-north iceland.] | objective: to investigate main causes of airborne immediate allergy in a region of one primary health care center with about 17,000 inhabitants in the northern part of iceland, both in general and with special reference to diagnoses. material and methods: totally 600 individuals with symptoms indicating airborne immediate allergy were investigated under the periode 1988-1995. skin prick test (spt) was used in a standardized way and with standardized solutions. histamine 10 mg/ml was used as a po ... | 1998 | 19667457 |
| biologic activity of dermatophagoides siboney and blomia tropicalis allergens in exposed and unexposed mite-allergic individuals. effect of patient selection on the biologic standardization of mite extracts. | this study aimed to investigate the influence of patient selection criteria, i.e., mite-allergic individuals exposed and not exposed to dermatophagoides siboney and blomia tropicalis, on the biologic activity of mite extracts. determination of the potency of mite extracts in vivo requires selection of patients with a clinical history of mite allergy. in scandinavia, there are some anamnestic criteria for mite allergy, whereas in the tropics, where patients are continuously exposed to high levels ... | 1999 | 10371100 |
| ige-mediated allergy to lepidoglyphus destructor in an urban population--an epidemiologic study. | the aim of this study was to estimate the prevalence of ige-mediated allergy to lepidoglyphus destructor and its clinical importance in reykjavik, iceland. | 1999 | 10485393 |
| identification and purification of a 16-kda allergen from psoroptes ovis (acarina: psoroptidae). | psoroptes ovis, (hering), the sheep scab mite, is the causative agent of an allergic dermatitis of sheep and cattle. recent studies of the host immune response to this ectoparasite have provided information that suggests control may be achieved by immune intervention. a significant effector in protection of the host from clinical lesions is host behavioral grooming. host grooming is believed to be intensified by a pruritic immediate hypersensitivity response to mite allergens. knowledge of poten ... | 1999 | 10534947 |
| studies on house dust mites in great cairo, egypt. | nowadays, house dust mites have showed themselves as one of the health problem worldwide. they have to be considered in the differential diagnosis of allergy particularly in children. house dust mites were collected from eight different areas in great cairo. nine species of mites were recovered from indoors. in order of abundance, they were dermatophagoides pteronyssinus, d. farinae, tyrophagous putrescentiae, acarus siro, cheyletus malaccensis, blomia kulagini, acheles graciles, ornithonyssus b ... | 1999 | 12561892 |
| evaluation of specific ige to the recombinant group 2 mite allergens lep d 2 and tyr p 2 in the pharmacia cap system. | several recombinant allergens have been shown to be potentially useful for diagnosis of ige-mediated allergy, but only a few recombinant allergens are at present commercially available in serological assays for detection of specific ige. the aim of this study was to evaluate the ige binding to the recombinant major dust mite allergens rlep d 2 and rtyr p 2 and compare it with the ige binding to the commercial mite extracts lepidoglyphus destructor and tyrophagus putrescentiae in the pharmacia ra ... | 1999 | 10529587 |
| occupational asthma in a grain worker due to lepidoglyphus destructor, assessed by bronchial provocation test and induced sputum. | occupational asthma (oa) can be a debilitating disease even when removal from the workplace is achieved. today, the "gold standard" in the assessment of oa is the bronchial provocation test (bpt). induced sputum is a non-invasive method of exploring airway inflammation which can provide additional information about such challenges and thus could be applied in oa diagnosis and monitoring. | 1999 | 10485394 |
| allergens from house dust and storage mites: similarities and differences, with emphasis on the storage mite lepidoglyphus destructor. | 2000 | 10848912 | |
| skin prick test and serological analysis with recombinant group 2 allergens of the dust mites l. destructor and t. putrescentiae. | the dust mites lepidoglyphus destructor and tyrophagus putrescentiae are important sources of allergen in farming environments. the major allergens of the dust mites l. destructor and t. putrescentiae have been cloned and expressed as recombinant proteins. | 2000 | 10792359 |
| sensitization to different mite species in german farmers: clinical aspects. | various mite species referred to collectively as house dust and storage mites are recognized worldwide as a cause of allergic airway disease. our study aimed to investigate the frequency of sensitization and potential importance of mite species in farmers using a broad mite spectrum. a total of 86 german farmers with rhinitis and/or asthma were studied by skin prick testing and/or enzyme allergosorbent test (east) with the following mites: blomia tjibodas, blomia tropicalis, blomia kulagini, gly ... | 2000 | 11206935 |
| sensitization to non-pyroglyphid mites in urban population of croatia. | determination of prevalence of sensitization to dermatophagoides pteronyssinus, dermatophagoides farinae (pyroglyphid mites), lepidoglyphus destructor, and tyrophagus putrescentiae (non-pyroglyphid mites) in urban continental croatia. | 2000 | 10810168 |
| serum eosinophil cationic protein in asthma of farmers. | there is need for relevant markers of bronchial inflammation in epidemiologic studies of asthma. serum eosinophil cationic protein (ecp) is a marker of eosinophil inflammation and asthma activity. we have studied serum ecp in atopic farmers with current asthma, in non-atopic asthmatics and in non-asthmatic, non-atopic controls. | 2000 | 10817397 |
| factors influencing the clinical picture and the differential sensitization to house dust mites and storage mites. | sensitization to house dust mites and storage mites has been studied in a number of papers, but several environmental factors and clinical conditions that differently affect sensitization to these mites are still controversial. the purpose of this study was to determine the influence of climatic conditions, occupation and patient age in the differential sensitization to house dust and storage mites, and also to search for possible different symptoms caused by each group of mites. eighty patients ... | 2000 | 11039840 |
| cloning of three new allergens from the dust mite lepidoglyphus destructor using phage surface display technology. | the dust mite lepidoglyphus destructor is a common species in europe and a major cause of dust mite allergy in rural surroundings, but it also contributes to dust mite allergy in urban areas. one major allergen, lep d 2, has been expressed as a recombinant protein and evaluated both in vivo and in vitro and shown to detect 60% or more of l. destructor-sensitized subjects. additional recombinant allergens are needed to obtain a reliable diagnostic tool for l. destructor allergy. the aim of this s ... | 2001 | 11168362 |
| allergenic cross-reactivity between the nematode anisakis simplex and the dust mites acarus siro, lepidoglyphus destructor, tyrophagus putrescentiae, and dermatophagoides pteronyssinus. | the nematode anisakis simplex is a common parasite on fish and other seafood. it is considered to be a food allergen and to induce ige-mediated reactions. allergenic cross-reactivity between a. simplex and other nematodes has been reported, as has cross-reactivity with arthropods: red mosquito larvae and german cockroach. we have here studied the allergenic relationship between a. simplex and four different dust-mite species. | 2001 | 11421925 |
| work-related skin symptoms and type i allergy among eastern-polish farmers growing hops and other crops. | 73 eastern-polish farmers growing hops and other crops were examined. they were questioned by a dermatologist and subsequently skin prick-tested with allergens of hops, grain dust, straw dust, hay dust, storage mites, and antigens of microorganisms typical for farm environment. results: 14 farmers (19.2%) complained of work-related skin symptoms, caused most often by hops (11%), followed by grain (5.6%), hay (5.5%) and straw (4.1%). five farmers (6.8%) complained of hand dermatitis, four (5.5%) ... | 2001 | 11426925 |
| the occurrence of mites in cereal-based foods destined for human consumption and possible consequences of infestation. | seven categories of cereal-based food products purchased at food retail outlets in uk were examined for the presence of mites by analysing 20 g samples using a flotation method. mites were found in 21% of 571 samples, which were examined soon after purchase, and in 38% of 421 samples, derived from the 571 samples which were examined after 6 weeks of storage in volunteers' homes. most of the samples where mites were detected had fewer than five mites. however, a few samples contained more than 20 ... | 2001 | 11523916 |
| evaluation of the acarofauna of the domiciliary ecosystem in juiz de fora, state of minas gerais, brazil. | from august 1999 to january 2000, samples of house dust were collected from 160 domiciles in the city of juiz de fora, state of minas gerais, brazil. in 36 of these domiciles kitchen samples were obtained. prevalence rate was 77.5%, varying according to the geographical sector. there were found 2,278 specimens of mites, with 1,530 (67.2%) in the adult stage and 748 (32.8%) in immature forms. the main species found were dermatophagoides pteronyssinus, d. farinae, euroglyphus maynei, blomia tropic ... | 2001 | 11685254 |
| t cell responses to recombinant isoforms, synthetic peptides and a mutant variant of lep d 2, a major allergen from the dust mite lepidoglyphus destructor. | the dust mite lepidoglyphus destructor is an important cause of allergic reactions to dust, especially in farming environments. two isoforms, recombinant (r)lep d 2.01 and rlep d 2.02, of the major allergen lep d 2, have previously been expressed as recombinant proteins. these isoforms differ 10.4% at the amino acid level. furthermore, a mutant form of lep d 2.01 (rlep d 2.6cys) with a highly reduced ige reactivity, has also been produced. | 2001 | 11737040 |