Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| new pentastomida, sambonia parapodum n. sp. from varanus salvator, and armillifer agkistrodontis n. sp. from agkistrodon acutus. | 1966 | 5936511 | |
| isolation of coagulant and anticoagulant principles from the venom of agkistrodon acutus. | 1967 | 5618974 | |
| purification and properties of the thrombin-like principle of agkistrodon acutus venom and its comparison with bovine thrombin. | 1971 | 5167421 | |
| purification and properties of the anticoagulant principle of agkistrodon acutus venom. | 1972 | 5069588 | |
| purification and properties of the coagulant and anticoagulant principles of agkistrodon acutus venom. | 1972 | 4512914 | |
| the effect of the purified anticoagulant principle of agkistrodon acutus venom on blood coagulation. | 1973 | 4721930 | |
| inhibition of the thrombin-like principle of agkistrodon acutus venom by group-specific enzyme inhibitors. | 1974 | 4439419 | |
| purification and characterization of the fibrinolytic principle of agkistrodon acutus venom. | by means of deae-sephadex a-50 column chromatography, agkistrodon acutus venom was separated into twelve fractions. the fibrinolytic activity was concentrated in fraction 9. this fraction was rechromatographed on sephadex g-75 three times and a single peak was obtained. the patterns of microzone and disc electrophoresis also showed a single band. a single, symmetrical boundary with a value of 2.44 s was obtained by ultracentrifugation, the molecular weight of which was estimated to be 24 100, an ... | 1976 | 8112 |
| the effects of the purified thrombin-like and anticoagulant principles of agkistrodon acutus venom on blood coagulation in vivo. | 1976 | 1258068 | |
| [enzymochemical studies on snake venoms. ii. purification of lethal protein ac1-proteinase in the venom of agkistrodon acutus (author's transl)]. | 1977 | 560456 | |
| in vivo effects of the purified thrombin-like and anticoagulant principles of agkistrodon acutus (hundred pace snake) venom. | 1978 | 153013 | |
| [enzymochemical studies on snake venoms. iv. purification of lethal protein ac2-proteinase in the venom of agkistrodon acutus (author's transl)]. | 1978 | 739392 | |
| [enzymochemical studies on snake venoms. iii. purification and properties of arginine esterase which posesses clotting activity in the venom of agkistrodon acutus (author's transl)]. | 1978 | 712547 | |
| [enzymochemical studies on snake venoms. v. purification of lethal protein ac3-proteinase in the venom of agkistrodon acutus (author's transl)]. | 1979 | 397338 | |
| the clotting activity of the thrombin-like enzyme of agkistrodon acutus (hundred-pace snake) venom. | 1979 | 473246 | |
| [enzymochemical studies on snake venoms. vi. purification and properties of anticoagulant factors of anticoagulant 1 and 2 in the venom of agkistrodon acutus (author's transl)]. | 1980 | 7441491 | |
| [enzymochemical studies on snake venoms. vii. purification of hemorrhagic principle ac4-proteinase in the venom of agkistrodon acutus (author's transl)]. | 1980 | 7003094 | |
| [clinical observation of 21 cases of agkistrodon acutus (guenther) bite complicated by disseminated intravascular coagulation (author's transl)]. | 1981 | 7341095 | |
| purification and characterization of hemorrhagic components from agkistrodon acutus (hundred pace snake) venom. | 1981 | 7302954 | |
| agkistrodon acutus snake venom inhibits prothrombinase complex formation. | 1981 | 7302928 | |
| presence of zinc in proteolytic hemorrhagic toxin isolated from agkistrodon acutus venom. | 1. hemorrhagic toxin (ac1-proteinase) was isolated from the venom of agkistrodon acutus (formosa) and the zinc content was determined (1.15 mol/mol protein). the results we extensively compared with hemorrhagic toxin e of crotalus atrox venom (u.s.a.). comparable results were obtained for zinc content, hemorrhagic and proteolytic activities for native hemorrhagic toxins from two different venoms. it is of interest that the zinc content of hemorrhagic toxins is identical even though the venoms ar ... | 1982 | 6125319 |
| [anti-ophidic toxin action of agkistrodon acutus serum]. | 1982 | 6219541 | |
| 111 cases of snake-bite (agkistrodon acutus guenther) treated by combined traditional chinese and western medicine. | 1982 | 6765699 | |
| [isolation of venom from agkistrodon acutus and assay of biological activities of thrombin-like enzyme and other fractions (author's transl)]. | 1982 | 6211897 | |
| purification and partial characterization of hyaluronidase from five pace snake (agkistrodon acutus) venom. | a hyaluronidase (ec 3.2.1. 35) was isolated and purified from agkistrodon acutus venom. the purification procedure included cm-sephadex c-50 chromatography, gel-filtration on sephadex g-75 and cm-sephadex c-25 chromatography. the purified preparation of the enzyme was homogeneous on polyacrylamide gel electrophoresis at ph 4.3, a 45-fold purification being obtained. the hyaluronidase was a glycoprotein (positive pas staining) with a molecular weight of 33,000 and a pi of 10.3. no hemorrhagic act ... | 1982 | 7164113 |
| effect of calcium on proteolytic activity and conformation of hemorrhagic toxin i from five pace snake (agkistrodon acutus) venom. | aahi, a proteolytic hemorrhagic toxin from a. acutus venom, contains one g-atom zn per mole protein and 2 g-atoms ca per mole protein. aahi is activated by calcium and slightly inhibited by zinc. when treated with edta, aahi is completely inactivated. when dialyzed against 1,10-o-phenanthroline, 50-80% of the metal content and activities are lost, while 90% of the hemorrhagic and proteolytic activities are retained when dialyzed against 1,10-o-phenanthroline containing 5mm ca2+. the results sugg ... | 1984 | 6395446 |
| comparative study of agkistrodon acutus venoms from china and taiwan. | three hemorrhagic toxins (ac1-, ac2- and ac3-proteinases) were isolated from the lyophilized venom of agkistrodon acutus from china using gel filtration on a sephadex g-75 column, followed by chromatography on diethylaminoethyl (deae)-sephadex a-50, deae-sephacel and deae-cellulose. homogeneity was established by the formation of a single band in acrylamide gel electrophoresis, isoelectric focusing and sodium dodecyl sulfate (sds) acrylamide gel electrophoresis. three hemorrhagic toxins possesse ... | 1984 | 6149878 |
| classification of agkistrodon species in china. | the wide geographical distribution of agkistrodon and the slight morphological differences among the snakes of the genus agkistrodon in china have posed a problem to taxonomists. we have employed polyacrylamide gel electrophoresis and immunological diffusion techniques for comparison of the venoms of different species and subspecies of agkistrodon from various localities. the electrophoretic patterns of the proteins of the venoms were different from each other, but showed certain relations withi ... | 1984 | 6426095 |
| purification of a proteinase (ac5-proteinase) and characterization of hemorrhagic toxins from the venom of the hundred-pace snake (agkistrodon acutus). | ac5-proteinase (15.2 mg) was isolated from agkistrodon acutus venom (1 g) by column chromatography on sephadex g-75, cm-sephadex c-50 and cm-cellulose. ac5-proteinase was homogeneous by disc electrophoresis on polyacrylamide gel at ph 4.3 and also by sds-disc polyacrylamide gel electrophoresis. ac1-, ac2-, ac3- and ac5-proteinases possessed lethal and hemorrhagic activities, but ac4-proteinase had no lethal activity. these activities were inhibited completely by ethylenediaminetetraacetic acid ( ... | 1984 | 6433514 |
| studies on the chemical modification of hemorrhagic toxin i from five pace snake (agkistrodon acutus) venom. | the chemical modification of hemorrhagic toxin i (aahi) from agkistrodon acutus has been studied. inactivation was observed upon modification of 3 out of 7 histidine residues with diethyl pyrocarbonate. the his residues are deblocked, accompanied by a return of activity, upon treatment with neutral hydroxylamine. the circular dichroism and fluorescence spectra of diethyl pyrocarbonate inactivated toxin and the native toxin are the same, indicating that modification with diethyl pyrocarbonate doe ... | 1985 | 4024138 |
| [the sandwich elisa for the thrombin-like enzyme of agkistrodon acutus venom]. | 1986 | 3751595 | |
| characterization of ac3-proteinase from the venom of agkistrodon acutus (hundred-pace snake). | ac3-proteinase from the venom of agkistrodon acutus was isolated in a homogeneous form by a previously published method. ac3-proteinase possessed lethal, hemorrhagic, caseinolytic, azocaseinolytic, dimethylcaseinolytic and hide powder azure hydrolytic activities. these activities were inhibited when ac3-proteinase was incubated with the metal chelators ethylenediaminetetraacetic acid (edta), ethyleneglycol-bis-(beta-aminoethyl ether)-n,n'-tetraacetic acid (egta), tetraethylenepentamine (tep), 1, ... | 1986 | 3539657 |
| platelet aggregation inhibitors from agkistrodon acutus snake venom. | among all the purified components from a. acutus venom, including adpase, 5'-nucleotidase, phospholipase a2 and fibrinogenases, only the venom adpase (50-100 micrograms/ml) shows marked inhibitory action on adp (10 microm)-, collagen (10 micrograms/ml)- and sodium arachidonate (100 microm)-induced platelet aggregations of rabbit platelet-rich plasma. the venom 5'-nucleotidase (100 micrograms/ml) inhibited adp-induced platelet aggregation by 31 +/- 4% (n = 4, p less than 0.05). fibrinogenolytic e ... | 1986 | 3031852 |
| [purification and characterization of depressor component of agkistrodon acutus venom]. | 1988 | 3195344 | |
| comparison of the platelet aggregation induced by three thrombin-like enzymes of snake venoms and thrombin. | platelet aggregation induced by three thrombin-like enzymes of snake venoms was compared with that by thrombin. acutin was isolated from agkistrodon acutus venom and thrombocytin and batroxobin were from bothrops atrox venom. the fibrinogen-clotting activities were 700, 170 and 7 u/mg for batroxobin, acutin and thrombocytin, respectively. they induced aggregation and atp release of washed rabbit platelets. the aggregating activity of thrombin was 10(2), 10(4) and 10(5) times more potent than tho ... | 1988 | 3291184 |
| characterization of clotting factors from agkistrodon acutus venom. | 1. four clotting factors, cf-1(c), cf-2(c), cf-1(t) and cf-2(t) were isolated from agkistrodon acutus (collected on mainland china and taiwan) venom by komori et al. (1987). it was reported that all factors possessed coagulant activity in the conversion of fibrinogen to fibrin, although they showed different chemical properties and antigenicities. 2. their role in the clot formation system was clarified and compared with that of thrombin. clotting factors from a. acutus venom released only fibri ... | 1988 | 2896611 |
| properties of nad glycohydrolase purified from five-pace snake (agkistrodon acutus) venom. | nad glycohydrolase (nadase) (e.c. 3.2.2.5) from five-pace snake (agkistrodon acutus) venom was purified to electrophoretic homogeneity through a 4-step isolation procedure, including column chromatography using deae-sephadex a-50, sephadex g-75, cm sephadex c-50 and sephadex g-100. the final product was 11.8-fold purified with a 3.9% yield. the pure enzyme showed maximal activity at about 40 degrees c with optimal ph at 7.5. it was a glycoprotein with a pi of 7.6. its mol. wt was respectively 98 ... | 1988 | 2845606 |
| isolation of an acidic phospholipase a2 from the venom of agkistrodon acutus (five pace snake) and its effect on platelet aggregation. | a phospholipase a2 from the venom of the snake agkistrodon acutus was purified by immunoaffinity chromatography and fast protein liquid chromatography (fplc) as a single band by page and sds-page. the estimated mol.wt was 16,400 by sds-page and 16,900 by gel filtration and the isoelectric point was 4.9. the ten n-terminal amino acid residues are homologous to those of the acidic phospholipases a2 from other crotalid venoms. the purified enzyme showed a potent inhibitory effect on platelet aggreg ... | 1989 | 2749764 |
| cross-neutralization of thrombin-like enzymes in snake venoms by polyvalent antivenoms. | five polyvalent antivenoms (crotalidae; orient, north, central and south africa) were tested for their ability to neutralize the thrombin-like activity of snake venoms (bitis gabonica, agkistrodon acutus, bothrops asper, b. atrox, crotalus adamanteus). considerable cross-neutralization was observed. anti-coagulase antibodies were isolated from an antivenom by affinity chromatography using a purified enzyme from bitis gabonica venom. these antibodies neutralized the activity of most snake venom c ... | 1989 | 2629180 |
| [coronary thrombolysis with defibrase]. | clinical use of defibrase (df), a fibrinolytic agent from venom of agkistrodon acutus, was investigated in patients with acute myocardial infarction (ami). patients with ami were randomized to tow groups, one receiving conventional therapy and df, the other a control group having conventional treatment only. the patients in the df group received intravenous df 0.05 u or 0.075 u/kg over 1 hour immediately after the attack and 0.025 u/kg over 4 hours on the 5 th and 10 th day. the control patients ... | 1990 | 2209234 |
| a pathologic study on the inhibitory effects of a herbal medicine against the glomerular lesion induced by agkistrodon venom in mice. | the inhibitory effects of a prescription of herbal medicine, tentatively named p-3, were studied pathologically in an experimental model of the glomerular lesion induced by purified snake agkistrodon acutus venom proteinase (ac1-p) in mice. ac1-p was intravenously inoculated at a single ld50 dose. in the treated group, mice were intraperitoneally injected with an extract of p-3 at a designated time once every two days from 2 days before to 1 week after ac1-p inoculation. the control group mice w ... | 1991 | 1830797 |
| characterization of ac1-proteinase from the venom of agkistrodon acutus (hundred-pace snake) from taiwan. | 1. ac1-proteinase from the venom of agkistrodon acutus was isolated in a homogeneous form by a previously published method. 2. ac1-proteinase possessed lethal, hemorrhagic, caseinolytic, azocaseinolytic, azoalbumin hydrolytic and hide powder azure hydrolytic activities. 3. the toxin also hydrolyzed the oxidized b chain of insulin and fibrinogen. the cleavage sites in the oxidized b chain of insulin were identified as ala(14)-leu(15) and tyr(16)-leu(17). the a alpha chain of fibrinogen was digest ... | 1991 | 2044839 |
| [the influence of chinese agkistrodon acutus enzyme (caae) on the functions of washed human platelets]. | the effects of the activation and influence of caae on washed human platelets were investigated. our findings were: (1) caae could induce aggregation and alpha-granule release reaction in the washed human platelet suspension; thus it could activate the platelets; (2) the aggregation effect of caae was inhibited by albumin and affected by ca2+, mg2+ concentration; (3) heparin and/or at iii did not inhibit the aggregation activation of caae, but alpha 2mg markedly inhibited it; (4) the aggregation ... | 1991 | 1814829 |
| immunological properties of the fibrinolytic enzyme (fibrolase) from southern copperhead (agkistrodon contortrix contortrix) venom and its purification by immunoaffinity chromatography. | an antibody to the fibrinolytic enzyme in southern copperhead venom was produced by immunizing rabbits with chromatographically purified enzyme. the antibody was purified from rabbit blood by ammonium sulfate fractionation and protein-a affinity chromatography. the purified antibody reacted only with the fibrinolytic enzyme in southern copperhead venom as demonstrated by immunodiffusion and immunoelectrophoresis. western immunoblotting revealed that several snake venoms, including agkistrodon pi ... | 1991 | 1926169 |
| [alpha 2 megaloglobulin inhibits the activation of rabbit platelet by chinese agkistrodon acutus venom]. | an in vitro study of alpha 2-mengaloglobulin (alpha 2mg) in the inhibition of the activation of washed rabbit platelet by chinese agkistrodon acutus venom (caav) was reported. results showed that caav induced aggregation rate of washed rabbit platelet was positively related to the caav concentration, and the maximal aggregation rate was 43.8 +/- 9.9% at the concentration of 100 micrograms/ml. meanwhile the release of serotonin (5-ht) and platelet factor 4(pf4) from the platelet reached maximum a ... | 1992 | 1284793 |
| identification of effective component from a traditional herbal medicine and the inhibitory effects on experimental glomerular lesion in mice. | effective constituents for renal disease were partially purified from a herbal prescription. the efficacy on renal diseases was examined using an experimental model system of glomerular lesion in mice induced by agkistrodon acutus venom (ac1-p). the extract of boiling water of the herbal prescription (p-3) was first fractionated by ether into an acidic fraction and a mixture of basic and neutral fractions. the acidic fraction was proved to be more effective and then further examined by thin laye ... | 1992 | 1558873 |
| coagulation factor x inhibitor from hundred-pace snake (deinagkistrodon acutus) venom. | deinagkistrodon acutus venom contains a collection of anticoagulant proteins that has been reported to prevent prothrombinase assembly (teng and seegers, 1981, thromb. res. 23, 255). a partial sequence indicates that these proteins are related to the functionally equivalent protein in trimeresurus flavoviridis (atoda et al., 1991, j. biochem. 106, 808). inhibition of prothrombinase, the complex of factors xa and va combined with phospholipids, is expressed in bovine, human, and rat plasmas as in ... | 1993 | 8310445 |
| the properties of the purified fibrinolytic principle from agkistrodon acutus snake venom. | 1993 | 193216 | |
| [experimental study of chinese agkistrodon acutus venom in activation of rabbit platelets in vivo]. | fourteen albino rabbits, male and female, were allocated randomly into two groups: control group which had intravenous infusion of normal saline, and experimental group which had intravenous infusion of chinese agkistrodon acutus venom (caav) 0.075 mg/kg. platelet was reduced, plasma pf4 activity and 5-ht concentration increased, 5-ht content in platelets was reduced in the experimental group 10, 20, and 50 minutes after the infusion. it had a significant difference (p < 0.01), compared with tha ... | 1994 | 8070769 |
| primary structure of ac1-proteinase from the venom of deinagkistrodon acutus (hundred-pace snake) from taiwan. | the entire amino acid sequence of ac1-proteinase from deinagkistrodon acutus venom was determined by using lysyl endopeptidase, metalloendopeptidase, trypsin and v8 protease. the hemorrhagic toxin had a typical zinc-chelating sequence his-glu-x-x-his found in thermolysin. | 1995 | 7655443 |
| functional and sequence characterization of agkicetin, a new glycoprotein ib antagonist isolated from agkistrodon acutus venom. offf2p4. | a new glycoprotein ib (gpib) antagonist, agkicetin, was purified from the venom of agkistrodon acutus and characterized. it is a disulfide-linked heterodimer consisting subunits of 15 and 14 kda. the subunits are homologous to each other and to other snake venom proteins of the c-type (ca(2+)-dependent) lectin superfamily. agkicetin behaved as a potent antagonist of von willebrand factor (vwf)-induced platelet agglutination (ic50 = 12.5 nm) and bound specifically to gpib of fixed platelets with ... | 1995 | 7755623 |
| [radiolabelling and assay of chinese agkistrodon acutus venom with carrier-free na 125i]. | chinese agkistroden acutus venom (caav) was radiolabelled with carrier-free na 125i by the method of iodogen. the specific activity and radiochemical purity for radiolabelled products were 4236.5 x 10(10) bq/mmol and 98%, respectively. each caav molecule carried 0.52 125i atom. physical and chemical characterization of radiolabelled caav was similar to unradiolabelled caav. binding analysis showed that 125i-caav was bound to platelet in a saturable manner. binding sites per platelet were 13,255 ... | 1995 | 7657328 |
| lys-49 phospholipase a2 homologs from venoms of deinagkistrodon acutus and trimeresurus mucrosquamatus have identical protein sequence. | the complete amino acid sequences of the lys-49 pla2s from the venom of deinagkistrodon acutus (from taiwan and china) and trimeresurus mucrosquamatus (taiwan habu) were solved by a facile cdna cloning and sequencing method. the deduced amino acid sequences of the lys-49 pla2s of both venoms are identical, suggesting close phylogenic relationship between this two snake species of different genera. in addition, by cloning and cdna sequencing, the mrna coding for a arg-49 pla2 homolog of low expre ... | 1996 | 8735248 |
| purification, crystallization and preliminary x-ray diffraction analysis of haemorrhagin iv from the snake venom of agkistrodon acutus. | haemorrhagin iv, a medium molecular weight haemorrhagin from the snake venom of agkistrodon acutus (aahiv), has been purified and crystallized. the molecular weight and isoelectric point of aahiv are 44 kda and pi 5.0, respectively. the crystal belongs to space group c222(1) with unit-cell dimensions of a = 124.2, b = 114.5, c = 98.4 a, and could o diffract x-rays to 3.0 a, resolution. there are one or two molecules in the crystallographic asymmetric unit. | 1996 | 15299713 |
| crystallization and preliminary x-ray diffraction studies of haemorrhagin i from the snake venom of agkistrodon acutus. | haemorrhagin i from the snake venom of agkistrodon acutus (aahi) has been crystallized using the hanging-drop vapour diffusion method. the crystals belong to space group p4(1)2(1)2 or p4(3)2(1)2 with unit-cell dimensions a = b = 63.61 and c = 95.69 a. there is one molecule in the asymmetric unit. data to 2.35 a resolution have been collected using a single-crystal. | 1996 | 15299746 |
| study on the action mechanism of hemorrhagin i from agkistrodon acutus venom. | 1996 | 8726075 | |
| molecular cloning and deduced primary structures of acidic and basic phospholipases a2 from the venom of deinagkistrodon acutus. | this paper presents the nucleotide sequences of the cdnas encoding three acidic phospholipases a2 and one basic phospholipase a2 from deinagkistrodon acutus venom. the deduced primary structure of the basic enzyme is closest to that of the basic neurotoxic enzyme from trimeresurus mucrosquamatus venom, while the acidic phospholipases from d. acutus have highest sequence similarity to that from agkistrodon halys pallas. the phylogeny of this monotypic species is discussed. | 1996 | 8931260 |
| [armillifer agkistrodontis disease: report of case]. | a case of armillifer agkistrodontis disease was reported. armillifer agkistrodontis is a species of the armillifer genus of the linguatulida order and parasitizing agkistrodon acutus. according to literature reviewed, there has been no report of this disease caused by infection of armillifer agkistrodontis in human being prior to this case all over the world. the clinical features of this disease are long-term high fever, abdominal pain, diarrhea, mild anemia, hepatosplenomegaly, eosinophilia in ... | 1996 | 9592342 |
| purification, characterization and conformational analysis of a haemorrhagin from the venom of agkistrodon acutus. | aahiv, a medium-sized toxin with a mol. wt of 44,000, a pi of 5.0 and a low cysteine content, was isolated from the venom of agkistrodon acutus by ion-exchange and gel filtration chromatography. it had haemorrhagic, lethal and caseinolytic activities, the last of which was inhibited by edta, zn(ch3coo)2 or cuso4. the circular dichroism spectrum at ph 7.0 showed two negative bands at 210 nm and 219 nm, corresponding to secondary structure contents of 18.2% alpha-helix, 31.0% beta-sheet, 17.2% bet ... | 1997 | 9080585 |
| crystal structure determination of alkaline haemorrhagin aah iii from snake venom of agkistrodon acutus. | the haemorrhagin aah iii isolated from the snake venom of agkistrodon acutus is one of the few alkaline ones in snake venoms. its crystals belong to space group p2(1)2(1)2(1) with a = 9. 573 4 nm, b = 4. 996 7 nm and c = 4.728 8 nm. its crystal structure was determined by the molecular replacement method according to the model of metalloproteinase adamalysin ii from eastern rattlesnake venom. the aah iii structure has been refined by prolsq. the final r factor was 0.254 and the rms deviations of ... | 1997 | 18762873 |
| crystal structures of acutolysin a, a three-disulfide hemorrhagic zinc metalloproteinase from the snake venom of agkistrodon acutus. | acutolysin a alias aahi, a 22 kda hemorrhagic toxin isolated from the snake venom of agkistrodon acutus, is a member of the adamalysin subfamily of the metzincin family and is a snake venom zinc metalloproteinase possessing only one catalytic domain. acutolysin a was found to have a high-activity and a low-activity under weakly alkaline and acidic conditions, respectively. with the adamalysin ii structure as the initial trial-and-error model, the crystal structures were solved to the final cryst ... | 1998 | 9784374 |
| [isolation and purification of the active principle from agkistrodon acutus snake venom by high performance ion-exchange liquid chromatography]. | the active component from agkistrodon acutus snake venom was further purified by high performance ion-exchange liquid chromatography over a protein-pak deae-8hr column(10.0 mm i.d. x 100 mm). the elution was operated with a linear gradient from 10% to 100% nacl-0.02 mol/l tris-hcl buffer(ph 8.0). the quantitative results were calculated as 45.41% based on peak area nomalization and the purity of the active component was 90% determined by electrophoresis. | 1998 | 11327007 |
| fluorescence analysis of the fibrinolytic principle (fp) from agkistrodon acutus venom. | the conformation and the properties of the fibrinolytic principle (fp) from agkistrodon acutus venom were studied by chemical modification and fluorescence spectroscopy. results showed that there are more than one tryptophan (trp) residue in the fp molecule and they are located in the more hydrophobic core, could be quenched by acrylamide (acr), a polarized quencher without electric charge. the collisional quenching constants of fp at different concentrations of acr were calculated in terms of s ... | 1998 | 12174266 |
| biochemical properties of bound-ca(2+) in fibrinolytic principle (fp) separated from agkistrodon acutus venom. | it has been determined that each fp molecular contains one ca(2+)-binding site. by the use of fluorescence probe tb(3+), the distance between tb(3+) and tryptophan (trp) residue was obtained to be 0.375. tb(3+) ion is coordinated with fp more strongly than ca(2+) ion, and can bind to fp and replace the ca(2+) ion in fp completely. | 1998 | 12174265 |
| purification, cdna cloning and molecular characteristic of a fibrinolytic enzyme from the venom of agkistrodon acutus. | a nonglycoprotein-like fibrinolytic enzyme ((fib-i) was purified from the crude venom of agkistrodon acutus by cm-sepharose cl-6b and deae-sepharose cl-6b ion exchange chromatography and then by fplc through superose 12 gel filtration. its molecular weight is about 23 kda and isoelectric point is near 6.0. it not only has fibrinolytic and caseinolytic activity, but also can hydrolyze baee. the local hemorrhagic activity was found in mice after the subcutaneous injection of this enzyme. edta can ... | 1998 | 9678189 |
| accutin, a new disintegrin, inhibits angiogenesis in vitro and in vivo by acting as integrin alphavbeta3 antagonist and inducing apoptosis. | endothelial integrins play an essential role in angiogenesis and cell survival. accutin, a new member of disintegrin family derived from venom of agkistrodon acutus, potently inhibited human platelet aggregation caused by various agonists (eg, thrombin, collagen, and, adenosine diphosphate [adp]) through the blockade of fibrinogen binding to platelet glycoprotein iib/iiia (ie, integrin alphaiibbeta3). in this report, we describe that accutin specifically inhibited the binding of monoclonal antib ... | 1998 | 9787163 |
| a new short chain rgd-containing disintegrin, accutin, inhibits the common pathway of human platelet aggregation. | a new short-chain disintegrin, accutin, was purified from the formosan agkistrodon acutus venom by using of gel filtration, ion exchanger and reverse phase hplc. the homogeneous protein is a 47-residue polypeptide with a molecular mass of 5241 da containing an arg-gly-asp sequence and seven cysteinyl residues at positions highly homologous to other disintegrins. accutin dose-dependently inhibited human platelet aggregation stimulated by adp, collagen, thrombin or the thromboxane analogue u46619 ... | 1998 | 9838213 |
| coagulation factor x-binding protein from deinagkistrodon acutus venom is a gla domain-binding protein. | factor ix/factor x-binding protein (ix/x-bp) is an anticoagulant isolated from the venom of trimeresurus flavoviridis (habu snake) and binds predominantly to factor ix. in this study, we isolated ix/x-bp-like proteins from the venom of deinagkistrodon acutus (hundred pace snake) with binding characteristics different from those of ix/x-bp. the complete amino acid sequence and binding characteristics of the main anticoagulant protein, named x-bp, were investigated. the concentrations of x-bp at h ... | 1998 | 9860851 |
| cdna cloning and expression of acutin. | acutin, a thrombin-like enzyme was purified from agkistrodon acutus venom in three steps by deae-sepharose cl-6b, superose 12 column on fplc and mono-q column chromatographies. its first 15 n-terminal amino acid residues sequence <viggvecdinehrfl> was then determined and the acutin cdna was isolated from venom gland total rna using rt-pcr. determination of its nucleotide sequence allowed elucidation of the amino acid sequence of mature peptide for the first time. the mature acutin has 233 amino ... | 1999 | 10049722 |
| purification, characterization, crystallization and preliminary x-ray diffraction of acuthrombin-b, a thrombin-like enzyme from agkistrodon acutus venom. | acuthrombin-b, a thrombin-like enzyme from agkistrodon acutus venom, has been isolated and purified to homogeneity by ion-exchange chromatography on deae-sepharose, gel filtration on sephacryl s-100 and fast performance liquid chromatography on deae-8hr. the protease is an acid protein (pi 6.0) consisting of two non-identical polypeptide chains (14.4 and 16 kda) and there is no disulfide bond between the subunits. its molecular weight is 27 kda as estimated by gel filtration on sephacryl s-100. ... | 1999 | 10329783 |
| purification and characterization of two fibrinogen-clotting enzymes from five-pace snake (agkistrodon acutus) venom. | from the snake venom of agkistrodon acutus, two proteases, acuthrombin-a and acuthrombin-c, were isolated and purified to homogeneity. they can cleave the human fibrinogen to release the fibrinopeptide a and fibrinopeptide b with specific activity of 120 and 370 nih units/mg, respectively; the fibrinogen-clotting activity can be inhibited distinctly by pmsf or dfp or edta, but not by heparin. the two proteases show also arginine-esterase activity hydrolyzing some synthetic substrates such as tam ... | 1999 | 10484747 |
| isolation and characterization of a neurotrophic factor-like substance from venom of agkistrodon acutus. | a neurotrophic factor-like substance from agkistrodon acutus was isolated by ion exchange and gel filtration chromatography and was found to be homogenous by electrophoresis. itsmolecular weight was estimated to be approximately 26 kd by gel filtration. a characteristic of the substance was that the protein consisted of two subunits which were bound one to another noncovalently. the analytical isoelectric focusing revealed its isoelectric point of 7.8. the biological activity of the substance wa ... | 1999 | 12136218 |
| molecular cloning and expression of the cdna for disintegrin from agkistrodon acutus. | in order to clone novel and valuable genes from natural resources and develop genetic engineering drugs, total rna was extracted from the venom gland of agkistrodon acutus by one step method. the cdnas for low molecular weight metalloproteinase were amplified by rt-pcr and cloned into the pgemt vector. their sequence were determined. one of the deduced amino acid sequences included a metalloproteinase and a disintegrin (acugrin) at the carboxyl terminus. this result further confirms that metallo ... | 1999 | 12114966 |
| expression and biochemical characterization of acidic phospholipase a(2)i from agkistrodon acutus. | a cdna encoding acidic phospholipase a(2)i(a.aapla(2)i)from agkistrodon acutus was inserted into a bacterial expression vector and effectively expressed in e.coli rr1. the protein was produced as insoluble inclusion bodies. after partial purification by washing the inclusion bodies with triton x-100, denaturing and refolding, the renatured recombinant protein was purified by fplc column superose(tm)12. the enzymatic acti-vity and platelet aggregation inhibiting effect of the expressed a.aapla(2) ... | 1999 | 12114959 |
| cloning and sequencing of genes encoding phospholipase a(2) from agkistrodon acutus. | synthetic oligonucleotides were used to amplify phospholipase a(2) (pla(2)) gene by rt-pcr from total rna of snake agkistrodon acutus venom gland. the pcr products were subcloned and positive clones were screened with acidic pla(2) gene from agkistrodon halys pallas. finally, four cdnas of pla(2) isoenzymes were isolated. their complete sequences were determined by bidirectional sequencing and their amino acid sequences were deduced. they were designated as a.aapla(2)i a.aapla(2)ii a.abpla(2) an ... | 1999 | 12142913 |
| purification, characterization, and cdna cloning of a new fibrinogenlytic venom protein, agkisacutacin, from agkistrodon acutus venom. | agkisacutacin is a new fibrinogenlytic protein from agkistrodon acutus venom. it consists of two heterologous subunits linked by an intersubunit disulfide bond. the cdnas encoding the two chains of agkisacutacin were cloned from a lambdagt11 cdna library of the snake venom gland and sequenced, including the leader peptides (23/23 amino acid residues) and mature subunits (129/123 amino acid residues). it is structurally related to the family of ix/x-binding protein (ix/x-bp)-like proteins and sho ... | 1999 | 10558903 |
| structure of acutolysin-c, a haemorrhagic toxin from the venom of agkistrodon acutus, providing further evidence for the mechanism of the ph-dependent proteolytic reaction of zinc metalloproteinases. | the structure of acutolysin-c, a haemorrhagic zinc metalloproteinase from the venom of agkistrodon acutus, has been analyzed and refined at 2.2 a resolution. the space group of the crystal is p2(1)2(1)2(1), with unit-cell dimensions a = 46.84, b = 49.52, c = 95.34 a. one molecule was found in each asymmetric unit. the phasing problem was solved by the molecular-replacement program amore. crystallographic refinement was performed using x-plor, leading to final r and free r factors of 0.176 and 0. ... | 1999 | 10531480 |
| molecular cloning and sequence analysis of cdna encoding haemorrhagic toxin acutolysin a from agkistrodon acutus. | by means of reverse transcription polymerase chain reaction, a full-length cdna of 1632 bp is amplified from the snake venom gland total rna of agkistrodon acutus. analysis of the nucleotide sequence indicates that the amplified cdna contains a complete open reading frame encoding 413 amino acid residues including signal peptide sequence, zymogen sequence and proteinase domain. the zymogen sequence contains pkmcgvt motif which is highly conserved in almost all venom metalloproteinases. the metal ... | 1999 | 10482389 |
| cdna cloning and sequence analysis of lys-49 phospholipase a2 from agkistrodon acutus. | total rna was extracted from venom glands of agkistrodon acutus. the cdna encoding lys-49 phospholipase a2 (pla2) was amplified by reverse transcriptional polymerase chain reaction (rt-pcr). the cdna was cloned into the pgemt-vector and sequenced. the open reading frame (orf) of lys-49 pla2 consists of 414 bp encoding 138 amino acids, which includes a signal peptide of 16 amino acids and a matured peptide of 122 amino acids. it shows 76% identity in amino acids with another reported lys-49 pla2. ... | 1999 | 10084123 |
| purification and characterization of a novel anticoagulation factor (acf ii) from the venom of agkistrodon acutus. | a novel anticoagulation factor (acf ii) was first isolated from the venom of agkistrodon acutus by deae-sephadex a-50, sephadex g-75 and cm-sephadex c-50 column chromatography. the purified acf ii showed a single band in page, s ds-page and ief-page, respectively. acf ii consisted of two peptide chains lin ke d together by disulfide bond(s) and the molecular weights of the two peptide cha ins were about 14.6 kd as determined by sds-page. the isoelectric point of acf ii was 7.0. acf ii possessed ... | 2000 | 12110921 |
| molecular cloning and sequence analysis of cdna encoding acutolysin c, a hemorrhagic metalloproteinase, from agkistrodon acutus. | a full-length cdna of 1 650 bp was amplified from the snake venom gland cdna library of agkistrodon acutus. analysis of the nucleotide sequence indicated that the amplified cdna contained a complete open reading frame encoding 417 amino acid residues including signal peptide sequence, zymogen sequence and proteinase domain. the zymogen sequence contained cgvt motif that was highly conserved in almost all venom metalloproteinases. the metalloproteinase domain contained a conserved signature zinc- ... | 2000 | 12075438 |
| purification and characterization of a platelet agglutinating inhibiting protein (agkisacutacin) from agkistrodon acutus venom. | a platelet agglutinating inhibiting protein (agkisacutacin) was isolated from the venom of agkistrodon acutus by deae sepharose fast flow and size exclusion chromatography. the purified product was a 29 kd protein composed of two disulfide bond-linked polypeptide chains of molecular weight of 14 kd, 15 kd, respectively. it completely inhibited ristocetin-induced platelet agglutination with an ic(50) value of 18.5 mg/l. it also inhibited thrombin-induced platelet aggregation with an ic(50) value ... | 2000 | 12058182 |
| [phylogenetic relationships among viperidae, crotalinae based on mitochondrial 12s rrna sequence variations]. | this paper analyzed the phylogenetic relationships and classification of pit vipers (viperidae, crotalinae) based on mitochondrial 12s rrna gene sequence variations. we have sequenced mtdna 12s rrna gene about 370 bp fragment from gloydius saxatilis emelianov, gloydius shedaoensis zhao, gloydius ussurriensis (emelianov), trimeresurus stejnegeri schmidt and deinagkistrodon acutus, gloydius brevicaudus (stejneger) from two different localities, respectively. combined with the sequence of dinodon s ... | 2000 | 11147345 |
| characterization, crystallization and preliminary x--ray diffraction analysis of acutohaemolysin, a haemolytic toxin from agkistrodon acutus venom. | acutohaemolysin, a phospholipase a(2) (pla(2)) from the venom of the snake agkistrodon acutus, has been isolated and purified to homogeneity by anion-exchange chromatography on a deae-sepharose column followed by cation-exchange chromatography on a cm-sepharose column. it is an alkaline protein with an isoelectric point of 10.5 and is comprised of a single polypeptide chain of 13 938 da. its n-terminal amino-acid sequence shows very high similarity to lys49-type pla(2) proteins from other snake ... | 2000 | 10930841 |
| purification and characterization of anticoagulation factors from the venom of agkistrodon acutus. | two anticoagulants from five-pace snake (agkistrodon acutus) venom, anticoagulation factor i (acf i) and anticoagulation factor ii (acf ii), have been purified by a multiple-step chromatography procedure of anion-exchange chromatography, gel permeation chromatography and cation-exchange chromatography. each of them is shown to be homogeneous as judged by page, sds-page and mass spectrometry. in vitro, both proteins show equivalent anticoagulant activity, and are devoid of proteolytic, esterolyti ... | 2000 | 10775752 |
| purification, cloning and sequence analyses for pro-metalloprotease-disintegrin variants from deinagkistrodon acutus venom and subclassification of the small venom metalloproteases. | acidic and basic hemorrhagic metalloproteases were purified from the venom of deinagkistrodon acutus (from fujian province, china) using gel filtration and anion exchange on fplc and reversed-phase hplc. their hemorrhagic activities and n-terminal sequences were characterized. extensive screening of the venom gland cdna after pcr amplification resulted in the identification and sequencing of a total of seven cdna clones encoding the multidomain precursors of six acidic and one alkaline low molec ... | 2000 | 10691973 |
| glycoprotein ib-binding protein from the venom of deinagkistrodon acutus--cdna sequence, functional characterization, and three-dimensional modeling. | agkicetin-c, a potent glycoprotein ib antagonist from the venom of the chinese pit viper, deinagkistrodon acutus, has been purified and characterized (5). it is a disulfide-linked heterodimer containing subunits of 132 and of 123 amino acid residues. herein, the complete amino acid sequences were resolved by cloning and nucleotide sequencing of the cdnas. the sequences of its subunits are homologous to those of other snake venom proteins of the c-type (ca2+-dependent) lectin superfamily. a three ... | 2000 | 10669165 |
| antithrombotic and thrombolytic activities of agkisacutacin, a snake venom proteinase, in experimental models. | the antithrombotic and thrombolytic activities of agkisacutacin (agk), a component isolated from agkistrodon acutus, were determined in vitro and in vivo. the models employed included chandler's model, arterio-venous shunt model and pulmonary embolus model. the effects of agkisacutacin on coagulation, plasma fibrinogen and platelet aggregation induced by collagen, adenosine diphosphate (adp) and thrombin were also investigated. the results showed that agkisacutacin can significantly inhibit thro ... | 2000 | 11827724 |
| [studies on hemorrhagic toxins from the venoms of trimeresurus mucrosquamatus, crotalus ruber ruber, vipera aspis aspis and agkistrodon acutus and arginine ester hydrolases from trimeresurus mucrosquamatus venom]. | venom samples were corrected from several poisonous snakes, such as bungarus multicinctus, trimeresurus mucrosquamatus, t. gramineus, t. flavoviridis, and agkistrodon acutus, and stored in a desiccator at room temperature for 25 to 31 years. then they were compared with fresh venoms as to their biological activities. the characteristic local symptoms produced by the bite of venomous snakes of crotalidae and viperidae are hemorrhage, necrosis and muscular degeneration. hemorrhagic toxins were pur ... | 2000 | 10774254 |
| hemostatic disturbances observed in patients with snakebite in south china. | to investigate the hematological disorders after snakebite, we measured the maximum platelet aggregation rate (mar), antithrombin iii (at-iii) activity, alpha(2)-plasmin inhibitor (alpha(2)-pi) activity, concentration of fibrinogen (fg) and fibrin degradation products (fdp) in 25 samples from 17 patients with snakebite in south china. the results obtained in the patients before application of antivenom and patients with ophiophagus hannah (oh.) bite were as follows: (1) the mean mar values were ... | 2000 | 10758271 |
| influential factors affecting prognosis of snakebite patients management: kaohsiung chang gung memorial hospital experience. | the objective of this study was to determine the most influential factors affecting the prognosis of snakebite patients in kaohsiung chang gung memorial hospital. | 2000 | 11126148 |
| pharmacological characterization and antithrombotic effect of agkistin, a platelet glycoprotein ib antagonist. | 1. agkistin, purified from the snake venom of formosan agkistrodon acutus, belongs to the family of c-type lectin gpib binding proteins. it is a heterodimeric molecule, consisting of alpha- (16.5 kda) and beta- (15.5 kda) subunits with a molecular mass of 32,512 daltons examined by sds - page and mass spectrometry. 2. in vitro, agkistin concentration-dependently inhibited ristocetin-induced human platelet agglutination and aggregation in the presence of vwf. it also inhibited txa2 formation and ... | 2001 | 11181425 |
| binding of anticoagulation factor ii from the venom of agkistrodon acutus with activated coagulation factor x. | anticoagulation factor ii (acf ii) from the venom of agkistrodon acutus has been identified as a binding protein to activated bovine coagulation factor x (fxa) by the method of polyacrylamide gel electrophoresis and high performance liquid chromatography. this protein formed a 1:1 complex with fxa in the presence of ca2+ ions, and the maximal binding of acf ii to fxa occurred at the concentration of ca2+ ions of about 1mm. the binding of ca2+ ions to acf ii was analyzed by equilibrium dialysis a ... | 2001 | 11384724 |
| purification, sequencing, and phylogenetic analyses of novel lys-49 phospholipases a(2) from the venoms of rattlesnakes and other pit vipers. | basic phospholipase a(2) homologs with lys49 substitution at the essential ca(2+)-binding site are present in the venom of pit vipers under many genera. however, they have not been found in rattlesnake venoms before. we have now screened for this protein in the venom of rattlesnakes and other less studied pit vipers. by gel filtration chromatography and rp-hplc, lys49-phospholipase-like proteins were purified from the venoms of two rattlers, crotalus atrox and crotalus m. molossus, and five nonr ... | 2001 | 11594738 |
| a novel tetrameric venom protein, agglucetin from agkistrodon acutus, acts as a glycoprotein ib agonist. | a novel platelet agglutination inducer, agglucetin, was purified from the formosan agkistrodon acutus snake venom. it migrated as a single band with an apparent molecular mass of 58.8 kda and two distinct bands of 16.2/14.5 kda under non-reducing and reducing conditions by sds-page, respectively. further confirmed by fplc, electrospray ionization mass spectrometry and 2d-page, native agglucetin exists as a tetramer composed of disulfide-linked alpha1, alpha2, beta1 and beta2 subunits. partial n- ... | 2001 | 11686327 |
| thrombolysis effect with fiia from agkistrodon acutus venom in different thrombosis model. | a fibrinolytic enzyme from agkistrodon acutus venom, called fiia, was tested for thrombolytic activity in animals. | 2001 | 11743889 |
| crystallization and preliminary x-ray analysis of jararhagin, a metalloproteinase/disintegrin from bothrops jararaca snake venom. | jararhagin is a toxic protein, isolated from the venom of the snake bothrops jararaca, which is composed of a metalloprotease domain coupled to a disintegrin/cysteine-rich domain. it induces local haemorrhage owing to the proteolytic digestion of the basement membrane of capillaries. jararhagin also cleaves the alpha(2)beta(1) integrin on the surface of platelets, thereby acting as a potent inhibitor of collagen-induced platelet aggregation. crystals of jararhagin were obtained by the vapour-dif ... | 2001 | 11468397 |
| the effect of calcium (ii) on the binding of anticoagulation factor i with activated coagulation factor x. | anticoagulation factor i (acf i) from the venom of agkistrodon acutus forms a 1:1 complex with activated coagulation factor x (fxa) in a ca2+-dependent fashion and thereby prolongs the clotting time. in the present study, the dependence of the binding of acf i with fxa on the concentration of ca2+ ions was quantitatively analyzed by hplc, and the result showed that the maximal binding of acf i to fxa occurred at concentration of ca2+ ions of about 1 mm. the binding of ca2+ ions to acf i was inve ... | 2001 | 11330346 |
| purification and biochemical characterization of f ii(a), a fibrinolytic enzyme from agkistrodon acutus venom. | a fibrinolytic enzyme, f ii(a), was isolated from agkistrodon acutus venom by ion-exchange chromatography and gel filtration. f ii(a) consisted of a single polypeptide chain with a molecular weight of 26,000 and an isoelectric point of 4.6. f ii(a) was shown to solubilize fibrin and fibrinogen. f ii(a) cleaved, primarily, the alpha chain of fibrinogen and fibrin followed by the beta chain, while the gamma chain was minimally affected. thus, the enzyme was an alpha,beta-fibrinogenase. the cleavag ... | 2001 | 11306123 |
| serine protease isoforms of deinagkistrodon acutus venom: cloning, sequencing and phylogenetic analysis. | the major coagulating fibrinogenase of deinagkistrdon acutus venom, designated acutobin, was purified by anion-exchange chromatography, gel filtration and reverse-phase hplc. approximately 80% of its protein sequence was determined by sequencing the various fragments derived from cnbr cleavage and digestion with endoprotease. extensive screening of the venom gland cdna species after amplification by pcr resulted in the isolation of four distinct cdna clones encoding acutobin and three other seri ... | 2001 | 11171091 |