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botrytis and sclerotinia. 191717799945
the order of death of organisms larger than bacteria.in a previous paper it has been shown that the logarithmic order of death of bacteria can be accounted for by the assumption of some very unstable molecules so essential for reproduction that the inactivation of only one such molecule per cell prevents reproduction and makes the cell appear "dead" according to the standard method of counting living bacteria. in the present paper dealing with the order of death of larger organisms, only a motile alga, chlamydomonas, is shown to have the same orde ...193119872588
pullulomyxa botrytis n. sp. 193916560256
the perfect state of botrytis squamosa walker. 194620997605
diseases of the gladiolus; botrytis rot of corms and its control. 194620997960
occurrence of botrytis fabae sardiña in england. 194720256153
[influence of light to the resistance of plantlets of lepidium sativum l. to botrytis cinerea pers]. 195213009908
[temperature re action of the respiratory process of botrytis cinerea]. 195214926653
[the formation of fermentation inhibitory substances by botrytis cinerea]. 195214954603
[inactivation with antibiotics of toxin produced by botrytis cinerea]. 195313083525
combined hormone-fungicide sprays for control of botrytis fruit-rot in glasshouse tomatoes. 195313087230
[contribution to the study of the biological value of calcium; broccoli leaves-brassica oleracea, l. var.: botrytis asparagoides. i..]. 195313134734
[biological value of calcium broccoli leaves: brassica oleracea, l. var.: botrytis asparagoides. ii..]. 195313134749
[biological value of calcium: broccoli leaves, brassica oleracea, l. var.: botrytis asparagoides. iii]. 195313155997
inheritance of mating type and a lethal factor in cosmarium botrytis var. subtumidum wittr. 195416578436
carbohydrate metabolism and oxalic acid synthesis by botrytis cinerea. 195416654653
[sclerotia of gray mould (botrytis cinerea persoon) on castor plant]. 195514387600
some effects of ultraviolet radiation on the pathogenicity of botrytis fabae, uromyces fabae and erysiphe graminis. 195713439161
[oxidative conversion of amino acids during interaction of cabbage with fungus botrytis cinerea]. 195813572395
ultrastructure of the collar of the choanoflagellate codonosiga botrytis (ehrenb.). 196113700095
orientation and locus of tropic photoreceptor molecules in spores of botrytis and osmunda.study of the tropic responses of botrytis cinerea and osmunda cinnamomea spores to blue light shows the photoreceptor molecules to be highly dichroic and oriented: in botrytis their axes of maximum absorption lie perpendicular to the nearby cell surface; in osmunda, parallel. the chief evidence lies in a comparison of their responses to plane polarized light-both germinate parallel to the vibration planes (defined by the axis of vibration of the electric vector and the axis of light propagation) ...196214450869
[a new disease of ficus elastica caused by a botrytis of the cinerea type]. 196214480580
uptake of amino-acids by the fungus botrytis fabae. 196214452313
an electron-microscope study of germination of conidia of botrytis cinerea. 196314072834
fungi associated with softening of bisulfite-brined cherries.softening of sound, calcium bisulfite-brined cherries was induced fairly quickly by brining them with cherries rotted by aspergillus niger, cytospora leucostoma, and penicillium expansum, but not with cherries rotted by a variety of other microorganisms, including alternaria sp., aspergillus oryzae, aureobasidium pullulans, botrytis cinerea, cladosporium sp., mucor racemosus, rhizopus stolonifer, and sclerotinia fructicola. rapid softening was correlated with the presence of a bisulfite-stable p ...196316349630
sorbic hydroxamic acid, an antifungal agent effective over a wide ph range.sorbic hydroxamic acid was prepared from sorbic acid by esterification and treatment with hydroxylamine (mp 133 to 135 c, pk(a) 8.8). its ultraviolet spectrum in acid solution had a single absorption maximum at 262 mmu; in alkaline solution the maximal absorption shifted to 255 mmu and significant absorption appeared at 280 to 300 mmu. at concentrations of 0.1% (w/v), sorbic hydroxamic acid prevented the growth of aspergillus niger, penicillium notatum, botrytis cinerea, cladosporium herbarum, a ...196316349636
biochemical tests indicative of reaction of castor bean to botrytis.pectic and cellulolytic enzymes caused browning and maceration of capsules of susceptible varieties of castor bean but not of resistant varieties. results of tests in which pericarp tissue of the capsule was treated with vanillin and h(2)so(4) and with an aqueous solution of fecl(3) and k(3)fe(cn)(6) showed that resistance to the hydrolytic enzymes was correlated with both a low concentration of flavanols and related compounds and the presence of oxidized compounds in injured tissue. resistance ...196317781088
[on the mechanism of action of botrytis cinerea toxin on plant cells]. 196414262628
vitamin k5 as a fungistatic agent.the effectiveness of vitamin k(5) in controlling the growth of different molds at varying ph levels in a culture medium, in tomato juice, and in several berry purees was studied. the molds studied were aspergillus, botrytis, hormodendrum, mucor, and penicillium. the results showed that vitamin k(5) was effective as a fungistatic agent at concentrations ranging from 0.006 to 0.02%.19655867645
[karyological studies on species and forms of the genus botrytis mich]. 19655894092
a quantitative study of cellular rheotropism.sparsely sowed, hence independent botrytis spores, which are fixed to the wall of a laminar flow chamber, tend to germinate downstream. for a velocity, v(infinity), one cell radius from the wall, of 0.1 to 1000 mu/second, this tendency, expressed as a percentage of perfect orientation, approximates 9 . log (10 v(infinity)). indirect proof is given that this rheotropic response is mediated by the convection across each cell of a diffusible stimulator emitted by it. analysis of the response indica ...196519431336
electron microscopy of botrytis cinerea conidia.buckley, patricia m. (university of california, davis), virginia e. sjaholm, and n. f. sommer. electron microscopy of botrytis cinerea conidia. j. bacteriol. 91:2037-2044. 1966.-sections of germinating and nongerminating botrytis cinerea conidia were examined with an electron microscope. uranyl acetate or lead citrate provided contrast between membranes and cytoplasm. membrane-bounded, dense inclusions previously unreported in dormant spores were termed "storage bodies." whorled structures, sphe ...19665949251
fungistatic effects of controlled atmospheres.the fungistatic effects of controlled atmospheres composed of increased co(2) and decreased o(2) was studied in a manner such that the condition of stored fruit was not a factor in the growth of the fungi. varying concentrations of o(2) and co(2) were used. the fungi used were botrytis alli, rhizopus nigricans, and penicillium expansum. the results showed that controlled atmospheres, within the limits of concentrations usable for fruit storage, are effective fungistatic agents.19665951331
[on the variability and taxonomy of varieties and forms of the species botrytis mich. i. studies on culture-conditioned variation of morphological properties of the species botrytis]. 19665953535
[on the variability and taxonomy of varieties and forms of the species botrytis mich. ii. studies on the variability of the culture type with constant culture conditions]. 19665953536
conidia of botrytis cinerea: labeling by fluorescent vital staining.as a model system, conidia of botrytis cinerea have been vitally stained with optical brighteners to facilitate their location in plant tissue.19664159926
on autotropism in botrytis: measurement technique and control by co(2).the mutual orientation of the germination of nearby pairs of botrytis spores growing in a simple, dilute medium was studied. if the medium is equilibrated with room air, they show a very strong tendency to germinate both toward each other and in a cis arrangement, i.e., toward the same side of the line joining their centers. if the medium is equilibrated with air enriched with 0.3% or 3% co(2), (i.e., 10 or 100 times the normal co(2) concentration) then the cells show an equally strong tendency ...196616656255
serine transhydroxymethylase of cauliflower (brassica oleracea var. botrytis l.): partial purification and properties.serine transhydroxymethylase (ec 2.1.2.1) has been purified 46-fold from cauliflower (brassica oleracea var. botrytis l.). the enzyme was completely dependent on the presence of tetrahydrofolic acid for the conversion of serine to glycine. the addition of pyridoxal phosphate gave a large increase in the reaction rate. a double ph optimum was observed with maxima at 7.5 and 9.5. the enzyme is specific for l-serine. the d-isomer is neither a substrate nor an inhibitor. the michaelis constants for ...196716656717
[a method of cultivating botrytis cinerea pers. for assaying various fungicides]. 19675629449
the effect of pollen grains on infections caused by botrytis cinerea fr. 19685705482
influence of phosphate compounds on certain fungi and their preservative effects on fresh cherry fruit (prunus cerasus, l.).studies were conducted to ascertain the retarding effects of four phosphate compounds (sodium hexametaphosphate, sodium tripolyphosphate, sodium tetraphosphate, and tetrasodium pyrophosphate) on molding of fresh cherries (prunus cerasus, l.). in vitro studies on their antimycotic effects against the most common fungal spoilers, penicillium expansum, rhizopus nigricans, and botrytis sp., were also carried out. sodium tetraphosphate appeared to be the most effective compound in preserving cherries ...19684295176
the association of carbohydrate changes in the shoot tip of cauliflower with flowering.changes in levels of sugars and starch in the shoot tip of cauliflower, brassica oleracea l. var. botrytis d. c. cv. main crop were studied during periods of growth which were inductive or non-inductive to flowering. flowering was induced by growing plants for 2 weeks under 16 hr of light at 5 degrees . during this period of floral induction there was a significant increase in sugar and starch content compared to that in vegetative plants grown at 20 to 26 degrees . sugar and starch content did ...196816656956
cytochemical evidence for the existence of peroxisomes in botrytis cinerea. 19694186256
[study of the kinetics and mechanisms of inactivation of the enzymes of the fungus botrytis cinerea pers with uv-rays]. 19704992016
oxidation of alcohols by botrytis cinerea.crude cell-free preparations of botrytis cinerea were found to oxidize straight-chain primary alcohols (except methanol), aromatic primary alcohols, and unsaturated primary alcohols. the resulting products were the corresponding aldehydes and an equal molar quantity of hydrogen peroxide.19715102778
the development of botrytis cinerea in irradiated strawberries during storage. 19715103389
[mechanism of action of uv-rays on the metabolism of amino acids and organic acids in the fungus botrytis cinerea pers]. 19715104359
mode of action of pollen in breaking resistance of vicia faba to botrytis cinerea. 19715106745
[effect of the fungus botrytis cinerea on incorporation of labeled amino acids by cabbage tissue ribosomes]. 19715167241
[effect of visible light and ultraviolet rays on the content of free amino acids and the amino acid composition of proteins of the fungus botrytis cinera pers]. 19715170636
an evaluation of 4-s-methyl-2-keto-butyric acid as an intermediate in the biosynthesis of ethylene.stimulation of ethylene production by cauliflower (brassica oleracea var. botrytis l.) tissue in buffer solution containing 4-s-methyl-2-keto-butyric acid is not due to activation of the natural in vivo system. increased ethylene production derives from an extra-cellular ethylene-forming system, catalyzed by peroxidase and other factors, which leak from the cauliflower tissue and cause the degradation of 4-s-methyl-2-keto-butyric acid. this exogenous ethylene-forming system is similar to the eth ...197116657663
growth and sporulation of botrytis convoluta with various carbon and nitrogen sources. 19725065013
growth of helminthosporia on media containing the fungicide benomyl and the assay of benomyl using botrytis cinerea. 19734735621
[protein synthesis in the mitochondria of healthy and botrytis cinerea infected cabbage tissues]. 19734798315
morphogenetic activity of cytochalasins, cyanein, and monorden in botrytis cinerea. 19734771700
the role of molybdenum in the synthesis of nitrate reductase in cauliflower (brassica oleracea l. var botrytis l.) and spinach (spinacea oleracea l.). 19744474017
observations on the ultrastructure of the choanoflagellate codosiga botrytis (ehr.) saville-kent with special reference to the flagellar apparatus.the ultrastructure of the choanoflagellate codosiga botrytis is described with particular reference to the flagellar appendages, the flagellar rootlet system, the transition zone, the basal body and accessory centrioles, and the stalk. the controversial early reports of flagellar appendages in this species have been confirmed and they have been detected in 2 further species, salpingoeca frequentissima and monosiga sp. the appendages consist of a delicate bilateral vane 2 mum wide on either side ...19751089676
role of glycosidases in the membranlytic, antifungal action of saponins.in studies on the membranlytic action of various saponins on mycelium of botrytis cinerea and rhizoctonia solani digitonin, alpha-hederin and tomatin caused considerable leakage of free amino acids, while aescin and theasaponin were less effective. cyclamin significantly damaged cell membranes of r. solani, but did not change the selective permeability of b. cinerea. cell membrane disruption was accompanied by an enzymatic conversion of saponins into their corresponding aglycones in cell membran ...19751171670
the effect of volatile and gaseous metabolites of swelling seeds on germination of fungal spores.effects of volatile and gaseous metabolites of swelling seeds of pea, bean, wheat, corn cucumber, tomato, lentil, carrot, red papper and lettuce on germination of spores of five genera of fungi were found to depend rather on the fungal than on the plant genus. germination of spores of botrytis cinerea, mucor racemosus and trichoderma viride was most severely inhibited. spores of verticillium dahliae were less sensitive and germination of spores of fusarium oxysporum was inhibited only in two cas ...19751176038
morphogenic effects of ramihyphin a in filamentous fungi.ramihyphin a at subfungistatic concentrations stimulates ramification of hyphae of filamentous fungi. stimulation of terminal ramification of hyphae that can be observed particularly in phytopathogenic fungi is most frequent. hyphae of microsporon canis, trichophyton mentagrophytes, blastomyces dermatitidis, coccidioides immitis and histoplasma capsulatum ramify intensively laterally. stimulation of the lateral ramification was observed in monilia fructigena, penicillium marneffei and penicilliu ...19751176042
purification of polygalacturonases produced by botrytis cinerea e-200. 19751240016
characterization of polygalacturonases of botrytis cinerea e-200. 19751240017
polygalacturonase of botrytis cinerea e-200 pers.a polygalacturonase (poly(1,4-alpha-d-galacturonide)glycanohydrolase, ec 3.2.1.15) was purified from the culture fluid of botrytis cinerea. the polygalacturonase preparation, homogeneous on the basis of disc-gel electrophoresis also showed pectinesterase activity. some properties of the purified polygalacturonase were studied. it had a molecular weight about 69 000. it was inactivated by p-chloromercuribenzoate, tetranitromethane and urea. a 50% loss in viscosity of sodium polypectate solution o ...1975804319
[properties of the laccase of botrytis cinerea].laccase from botrytis cinerea, secreted by the fungus into the grape berry, is responsible for the oxidation phynomena in musts and wines (oxidasic casse). from a fungus culture, the enzyme was extracted for a study of its properties: specificity; influence of inhibitors, ph and temperature; the results confirm that the enzyme from botrytis cinerea belongs to the laccase family, though it has some characteristic differences from the other laccases previously described.1975238748
light-induced synchronous conidiation in the fungus botrytis cinerea.botrytis cinerea pers. ex fr. in stationary liquid cultures conidiated asynchronously in darkness after 4 days' growth. synchronous conidiation was induced by irradiating dark-grown cultures with near-ultraviolet light for 12 h. the number of conidia increased very rapidly 10 h after the end of the photo-induction period, and conidiation was completed by the 14th hour. filter paper cultures of the fungus also showed synchronous conidiation upon irradiation with near-ultraviolet light, but the ra ...1976945324
bovine nasal granuloma in victoria -- a search for the causative allergens.cows with nasal granuloma showed skin sensitivity to a range of environmental allergens while clinically normal control animals showed little sensitivity. the allergens producing the greatest number of positive reactions included the pollens of dock, capeweed, clover, paspalum, sorrel, rye grass, pepper tree and wattle, the spores of penicillium, cladosporium, botrytis and rye grass rust and extracts of sheep's wool and mite. it is likely that different allergens are important in different anima ...1976938353
sensitivity distribution of phytopathogenic bacteria and fungi to antibiotics.the minimal inhibitory concentrations (mic) of various antibiotics and fungicides for erwinia carotovora, pseudomonas coronafaciens var. atropurpurea, p. lachrymans, alternaria mali, a. kikuchiana, pyricularia oryzae, botrytis sp. and sclerotinia sp. isolated from diseased plants in various localities of japan were examined to enable the isolates to be gruoped into sensitive and resistant strains. to minimize the effects of various variable conditions, mic of isolates were pooled for either 2 or ...19761033174
metabolism of the phytoalexin rishitin by botrytis spp. 1976988112
antibiotic-induced changes of mycelial growth of botrytis cinerea.the effect of antibiotics and metabolic inhibitors on mycelial growth of botrytis cinera was followed. inhibitors of protein synthesis, chloramphenicol, erythromycin and tetracycline inhibit growth or sporulation of botrytis cinera. ethidium bromide, 5-fluorouracil, phenylethylalcohol and k 20 cause granulation, vacuolization and undulation of hyphase. 2,4-dinitrophenol, boromycin, macrotetrolides, monensin, scopathricin and tx2 at subfungistatic concentrations induce intensive branching of hyph ...1976987979
comparative studies on polyguanylate polymerase and polyadenylate polymerase activities in the dna-dependent rna polymerase i fraction from cauliflower.the properties of poly(g) polymerase and poly(a) polymerase activities in the dna-dependent rna polymerase nucleosidetriphosphate: rna nucleotidyltransferase ec 2.7.7.6 i fraction from cauliflower (brassica oleracea var. botrytis) were comparatively investigated. the ph optimum, the effect of ionic strength, the effect of substrate concentration on the rate of synthesis, the effect of divalent metal ion concentration, and the time course of synthesis at different temperatures were all different ...19767554
[fungal spore content of the air in munich (author's transl)].a volumetric determination of seasonal variations in the fungal spore content of the air was carried out with andersen samplers (culture plates) in the center of the city of munich and 2.4 km (1.5 miles) west of it. cladosporium spores were predominant (60% altogether), followed by those of penicillium and aspergillus. up to 22 different genera were counted among the varieties. the spores of cladosporium, penicillium, aspergillus, botrytis, thysanophora and epicoccum are twice as densely represe ...1977403426
the fungal flora of loganberries in relation to storage and spoilage.botrytis cinerea, cladosporium spp., penicillium spp., aureobasidium pullulans, mucor mucedo, phoma state of didymella applanata, cryptococcus laurentii var. laurentii, c. albidus var. albidus, candida sake and sporobolomyces roseus were consistently present on freshly harvested loganberries. botrytis cinerea was the main spoilage organism with mucor mucedo of minor importance. the importance of benomyl-resistant strains of b. cinerea is discussed.1977557301
plant pyruvate dehydrogenase complex purification, characterization and regulation by metabolites and phosphorylation.the pyruvate dehydrogenase complex was purified from mitochondria of cauliflower, brassica oleracea var. botrytis floral buds to a specific activity of 5.4 mumol of nadh/min per mg of protein. the pyruvate dehydrogenase complex required coash, nad+, thiamine pyrophosphate and mg2+ for the oxidative decarboxylation of pyruvate. the kinetic analysis of the complex gave a series of parallel lines for all substrates. product interaction patterns showed that nadh is competitive with nad+; acetyl-coa ...1977922017
locus of blue and near ultraviolet reversible photoreaction in the stages of conidial development in botrytis cinerea.the effect of the blue and near ultraviolet reversible photoreaction on conidial development in botrytis cinerea was studied by observing microscopically selected conidiophores. conidiophore development was divided into six stages; when the developing conidiophores from stage 2 (i.e. a mature conidiophore) to stage 5 (i.e. a conidiophore with conidium initial) were exposed to blue light for a short time, conidiation was suppressed; the conidiophores already formed de-differentiated to 'sterile' ...1977556757
large-scale purification and subunit structure of dna-dependent rna polymerase ii from cauliflower inflorescence.dna-dependent rna polymerase ii (nucleosidetriphosphate:rna nucleotidyltransferase, ec 2.7.7.6) from cauliflower inflorescence (brassica oleracae, var. botrytis) was highly purified by polyethyleneimine treatment on a large scale. the solubilized enzyme was partially purified by polyethyleneimine fractionation and subjected to chromatography on deae-sephadex and phosphocellulose, and subsequently to sedimentation in a glycerol gradient. the specific activity (231 nmol/mg per 10 min) of this enzy ...1978623757
[new analytic criteria for the characterization of wines].isotopic analysis, determination of radioactivity are factors allowing a better characterization of wines. the analysis of the composition in stable isotopes of the constituants of wine can provide new analytic criteria for characterizing wines. thus, the concentration of 13c in ethanol can allow the disclosure of an addition of cane sugar or maize sugar to the musts. a study of the composition in stable isotopes of the water of wines of a certain region made it possible to show a correlation be ...1978573085
serological cross-reactivity among sporothrix schenckii, ceratocystis, europhium, and graphium species.ethanol-precipitable culture filtrate antigens of 100 strains of 75 species of the sporothrix-ceratocystis-europhium-graphium complex and 1 species of botrytis were examined for neutral sugar components and for serological cross-reactivity with s. schenckii rabbit antiserum and human sporotrichosis sera by capillary precipitin and double immunodiffusion assay. results revealed that cross-reactive species (60 of 77, ca. 80%) produced exoconidial forms and rhamnose- and mannose-containing polysacc ...197899369
detection of mutagens produced by fungi with the salmonella typhimurium assay.forty-one fungal isolates (one isolate per species) representing common plant pathogens and food crop contaminants were grown on sterile, polished rice and assayed for mutagenic activity in the salmonella typhimurium-microsome system. initially, single doses of aqueous and chloroform extracts of the moldy rice were assayed against the ta100 tester strain by incorporating extracts into the growth medium and by applying small quantities on disks placed on the agar surface. suspected activity was e ...1978354528
[purfication and molecular weight of an rna-dependant rna polymerase from brassicae oleracea var. botrytis].an rna-dependent rna polymerase has been completely purified from cauliflower inflorescences. analysis of the purified enzyme on sds-polyacrylamide gels showed one polypeptide chain with a molecular weight of 140,000 dalton. the enzyme is monomeric in its native state. the in vitro activity was completely dependent on added rna, the most efficient templates being poly (u), poly (u, c), poly (i) and viral rna.1978105817
the degradation of onion cell walls by extracellular enzymes of botrytis allii and sclerotium cepivorum [proceedings]. 1978565727
action spectrum for photosporogenesis in botrytis cinerea pers. ex fr.an action spectrum for photoinduced conidium formation in the fungus botrytis cinerea pers. ex fr. was determined by exposing colonies to monochromatic radiation obtained from a diffraction grating monochromator. wavelengths longer than 355 nm were ineffective even after exposures of 8 hours at intensities of 4,068 to 8,276 erg per cm(2) per second. colonies were exposed at 22.5 c from 226 to 355 nm at about 5 nm intervals. three prominent peaks of effectiveness occurred at about 231, 268, and 2 ...197816660370
incidence of fungal spores at the homes of allergic patients in an agricultural community. iii. associations with local crops.a predominantly agricultural community in california was surveyed for prevalent fungal spores during a 12-month period. alternaria, macrosporium and stemphylium were recovered during asparagus and strawberry harvesting times year-round. fusarium and botrytis were less frequently associated with the strawberry harvest and were recovered only during the first quarter of the year. epicoccum was recovered in the north end of the salinas valley in low numbers throughout the year and was strongly asso ...1979574745
[opportunistic fungi in the atmosphere].our knowledge in aerobiology has considerably increased in the last 20 years due to new trapping methods. the use of volumetric spore traps permits to accurately analyze the particles suspended in the air and their evolution, while the deposition method gives an incomplete picture of the real air content. the air microflora changes considerably during 24 h: some groups of spores occur in the morning, others in the afternoon and yeast-like organisms during the night. the most common fungi in the ...1979114434
herbicidins f and g, two new nucleoside antibiotics.a mutant of streptomyces saganonensis no. 4075, obtained with n-methyl-n'-nitro-n-nitrosoguanidine treatment, produced herbicidins f and g without herbicidins a and b. isolation of the antibiotics was performed by adsorption on resinous adsorbent followed by elution with aqueous meoh. herbicidin f was obtained as colorless needles after extraction of the eluate using methylene dichloride. purification of herbicidin g was completed with silica-gel chromatography to give a crystalline powder. phys ...1979511776
stimulation of de novo synthesis of l-phenylalanine ammonia-lyase in relation to phytoalexin accumulation in colletotrichum lindemuthianum elicitor-treated cell suspension cultures of french bean (phaseolus vulgaris).(1) the regulation of the accumulation of the isoflavonoid-derived phytoalexin phaseollin in cell suspension cultures of dwarf french bean (phaseolus vulgaris/ has been investigated. (2) an elicitor preparation from cell walls of colletotrichum lindemuthianum, the causal agent of anthracnose disease of french bean, caused a marked accumulation of phaseollin in the cultures. the elicitor induced phaseollin accumulation to a level of 60% that obtained with the artificial elicitor autoclaved ribonu ...1979476149
microbial transformations of natural antitumor agents. 9. o-demethylation of 9-methoxyellipticine.microbial transformation studies were conducted with 9-methoxyellipticine (1). several microorganisms including botrytis allii (nrrl 2502), cunninghamella echinulata (nrrl 1386), c. echinulata (nrrl 3655), and penicillium brevicompactum (atcc 10418) achieved o-demethylation of (1) in good yield. p. brevicompactum was used to prepare 9-hydroxyellipticine (4) from 1 for isolation and complete identification. high-performance liquid chromatography was used to verify the identity of the major metabo ...1979575547
the distribution of the nadph regenerating mannitol cycle among fungal species.the mannitol cycle is an important nadph regenerating system in alternaria alternata. the cycle is built up to the following enzymes: mannitol 1-phosphate dehydrogenase, mannitol 1-phosphatase, mannitol dehydrogenase and hexokinase. the net reaction of one cycle turn is: nadh + nadp+ + atp leads to nad+ + nadph + adp + pi. the enzymes needed for an operating cycle were found in aspergillus, botrytis, penicillium, pyricularia, trichothecium, cladosporium and thermomyces all genera belonging to fu ...19806782999
volatile and gaseous metabolites released by germinating seeds of lentil and maize cultivars with different susceptibilities to fusariosis and smut.the effect of volatile and gaseous metabolites released by germinating seeds of lentil cultivars more and less susceptible to fusariosis on the germination of spores of mucor racemosus, trichoderma viride, verticillium dahliae and botrytis cinerea was found to depend rather on the fungal genus than on the lentil cultivar. however, spores of fusarium oxysporum reacted more sensitively during germination to the presence of exudates of both cultivars, when the more susceptible lentil displayed a st ...19807189738
air-borne fungi in the air of barcelona (spain). iv. various isolated genera.during a two-year survey on the air-borne fungi in the atmosphere of barcelona (spain), the following genera were isolated in decreasing order: aureobasidium, rhizopus, mucor, arthrinium, phoma, fusarium, trichoderma, and botrytis.19807190223
[occurrence of an extracellular beta-(1 to 3)-glucanase from botrytis cinerea].like other fungi, botrytis cinera produces an exocellular beta-(1 leads to 3)-glucanase. this constitutive enzyme is produced only when botrytis is cultivated in a glucose depleted medium. the enzyme is precipitated by ammonium sulfate from 20 to 80% saturation. maximum activity is at ph between 5 and 6 and temperature between 40 and 50 degrees c; stability is good up to 30 degrees c. this enzyme cannot be applied to the treatment of wines containing botrytis glucan.19806771030
characterization of two dna polymerases from cauliflower inflorescence.two distinct dna polymerases, a and b, isolated from a rapidly growing apical tissue of cauliflower (brassica oleracea var. botrytis) inflorescence have been characterized, and compared with dna polymerases, alpha and beta, from mouse myeloma. polymerase-a bears a strong resemblance to polymerase-alpha from mammalian cells in all properties examined. the character of polymerase-b is also quite similar to polymerase-beta of mammalian cells in chromatographic elution properties, template-primer ut ...19807390985
[antifungal activity of 3,3'-diindolyl-2,2'-tetrasulfide].the compound 3,3'-diindolyl-2,2'-tetrasulfide was studied for in vitro and in vivo fungistatic activity against various fungi causing human and plant diseases. the compound showed marked activity in vitro especially against dermatophytes and in the agrarian field against botrytis cinerea. when the low phytotoxicity of the compound is taken into account, it is concluded that the compound has considerable potential as an anticryptogam.19817308457
mycoparasitism of sclerotial fungi by teratosperma oligocladum.sclerotia of sclerotinia minor were parasitized by teratosperma oligocladum, a recently described dematiaceous hyphomycete. the mycoparasite was cultured on living sclerotia placed on water agar and on sclerotia in moist sand. it grew poorly on several common laboratory media but growth in vitro was enhanced by supplements of soil extract and, especially, by aqueous extracts of sclerotia. sclerotia of s. minor, s. sclerotiorum, s. trifoliorum, sclerotium cepivorum, and botrytis cinerea were para ...19817198002
[molds in foods of the czechoslovak socialistic republik (author's transl)].the mycoflora of 13.527 g of food powders which were normal upon sensory evaluation as well of 22.017 pieces of surface sterilized grains and nuts was examined in the czechoslovak socialist republic. 147.027 colonies of 44 genera of molds were isolated and identified from samples. colonies of penicllium sp., aspergillus sp., and cladosporium sp. occured most often, less often or quite infrequently rhizopus sp., mucor sp., absidia sp., alternaria sp., circinella sp., trichoderma sp., botrytis sp. ...19817269803
hormone effects on cosmarium botrytis cell division.the synchronous generation time for cosmarium botrytis menegh. was 48 h at ph 8.0, and 72 h at ph 5.3. cells divided 2 to 3 prior to the end of the dark phase in a 15 h light/9 h dark cycle at 21 degrees c. kinetin at concentrations of 0.01 to 1.0 mg/ml, added to cells at the beginning of the light cycle, eliminated the initial lag phase at both ph 5.3 and ph 8.0, and all cells divided until 24 h. indole-acetic acid in concentrations of 0.03 to 0.06 mg/ml decreased the lag phase to less than 48 ...19817273854
activation kinetics of nad-dependent malic enzyme of cauliflower bud mitochondria.nad-dependent malic enzyme (ec 1.1.1.39) was obtained from isolated mitochondria of cauliflower buds (brassica oleracea l., var. botrytis). the nad-linked activity is accompanied by a minor nadp-linked activity. some contaminant nadp-malic enzyme from the supernatant and the plasma membrane is usually present in crude mitochondrial preparations. nad-dependent malic enzyme has been purified 38-fold by ammonium sulfate fractionation and gel permeation chromatography, to a specific activity up to 2 ...198116662073
persistence of carbaryl residues in and on unprocessed and processed cauliflower curds.carbaryl was applied three times at 15 days interval to cauliflower (brassica oleracea var. botrytis l.), and its persistence was determined spectrophotometrically at 0, 1, 3, 6, 10, and 15 days after the first and last treatments. in unprocessed cauliflower the prescribed tolerance level for carbaryl residues of 5 ppm was only reached on the sixth day, in processed cauliflower on the third day after application. pretreatment with washing + boiling/cooking on the other hand led to a considerable ...19826807280
cell wall degradation in the autolysis of filamentous fungi.a systematic study on autolysis of the cell walls of fungi has been made on neurospora crassa, botrytis cinerea, polystictus versicolor, aspergillus nidulans, schizophyllum commune, aspergillus niger, and mucor mucedo. during autolysis each fungus produces the necessary lytic enzymes for its autodegradation. from autolyzed cultures of each fungus enzymatic precipitates were obtained. the degree of lysis of the cell walls, obtained from non-autolyzed mycelia, was studied by incubating these cell ...19826219290
[antifungal and antimicrobial activity of beta-ionone and vitamin a derivatives].the antifungal and antimicrobic activity of some derivatives of beta-ionon and vitamin a was studied. these compounds (citral, pseudo-ionon. beta-ionon aldehyde c14, ketone c18 and its derivatives--4,18-diketone, alcohol c18, semicarbazide ketone c18), as well as vitamin a and its derivatives--retinal, acetate, retinoic acid--differ in composition, structure and substituents of c-atoms in beta-ionon ring and in polyenoic chain. fusarium solani, botrytis cenerea and verticillum dahliae ii, race 4 ...19836364128
a volumetric-aerobiologic study of seasonal fungus prevalence inside and outside dwellings of asthmatic patients living in northeast netherlands.air sampling with an andersen sampler was carried out over a period of 21 mo, in 28 houses of asthmatic patients with an allergy for moulds. inside, the livingroom, bedroom, and kitchen were studied, and an additional outside sample was always taken. indoors, penicillium showed the highest concentration, especially in the autumn and winter months, while aspergillus and yeasts were also found frequently. the outside air was dominated by cladosporium and alternaria during summer months and botryti ...19846507952
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