Publications

isolation and characterization of a beta-tubulin-encoding gene from colletotrichum gloeosporioides f. sp. aeschynomene.	colletotrichum gloeosporioides f. sp. aeschynomene (c.g.a.) is a fungal pathogen of legumes and is used as a commercial mycoherbicide for rice and soybeans. as an initial study to potentially improve the utility of this fungus and develop a gene transfer system, a beta-tubulin (beta tub)-encoding gene (tub1) was isolated, cloned and sequenced. the coding sequence and deduced amino acid sequence of the c.g.a. tub1 gene was highly homologous to the tub1 gene of colletotrichum graminicola. southern ...	1993	8440473
isolation, characterization, and expression of a second beta-tubulin-encoding gene from colletotrichum gloeosporioides f. sp. aeschynomene.	colletotrichum gloeosporioides f. sp. aeschynomene is a fungal plant pathogen of aeschynomene virginica. a beta-tubulin-encoding gene (tub2) from this pathogen was cloned and sequenced. the deduced amino acid sequence of tub2 had a high degree of homology to other fungal beta-tubulins. a portion of tub2 from a benomyl-resistant c. gloeosporioides f. sp. aeschynomene mutant was also cloned and sequenced. a point mutation resulting in a glutamic acid-to-lysine substitution at amino acid 198 likely ...	1994	7993097
gene expression analysis during conidial germ tube and appressorium development in colletotrichum trifolii.	preinfection development in colletotrichum spp. exhibits three morphologies (conidia, germ tubes, and appressoria) and is directed by a complex interplay of environmental signals. germ tube morphogenesis for colletotrichum trifolii and the related fungus colletotrichum gloeosporioides f. sp. aeschynomene was shown to be partially dependent on a balance between self-germination inhibitors and environmental nutrients or cutin. the degree of responsiveness to these environmental signals was strikin ...	1997	16535630
indole-3-acetic acid biosynthesis in colletotrichum gloeosporioides f. sp. aeschynomene	we characterized the biosynthesis of indole-3-acetic acid by the mycoherbicide colletotrichum gloeosporioides f. sp. aeschynomene. auxin production was tryptophan dependent. compounds from the indole-3-acetamide and indole-3-pyruvic acid pathways were detected in culture filtrates. feeding experiments and in vitro assay confirmed the presence of both pathways. indole-3-acetamide was the major pathway utilized by the fungus to produce indole-3-acetic acid in culture.	1998	9835603
transformation of the bioherbicide colletotrichum gloeosporioides f. sp. aeschynomene by electroporation of germinated conidia.	a highly efficient and reproducible procedure for the transformation of the bioherbicide colletotrichum gloeosporioides f. sp. aeschynomene by electroporation of germinated conidia is reported. development of the procedure involved a detailed study of the germination process followed by extensive calibration of the electrical-pulse and selection conditions. optimization of the transformation protocol was facilitated by the use of the green fluorescent protein that helped in the identification of ...	1999	10447601
camp regulation of "pathogenic" and "saprophytic" fungal spore germination.	we report on the elucidation of two separate pathways of spore germination in a plant pathogenic fungus colletotrichum gloeosporioides f. sp. aeschynomene. conidia of the fungus can germinate either from one side or from both sides, depending on external conditions. in shake culture that includes an extract made up from fresh peas, the unicellular conidium divides and one of the two cells develops a germ tube. on a solid surface this germ tube differentiates an appressorium. in rich medium witho ...	2004	14761792
in planta production of indole-3-acetic acid by colletotrichum gloeosporioides f. sp. aeschynomene.	the plant pathogenic fungus colletotrichum gloeosporioides f. sp. aeschynomene utilizes external tryptophan to produce indole-3-acetic acid (iaa) through the intermediate indole-3-acetamide (iam). we studied the effects of tryptophan, iaa, and iam on iaa biosynthesis in fungal axenic cultures and on in planta iaa production by the fungus. iaa biosynthesis was strictly dependent on external tryptophan and was enhanced by tryptophan and iam. the fungus produced iam and iaa in planta during the bio ...	2004	15006816
[cloning of -tubulin gene and their correlation with conferred carbendazim resistance of colletotrichum gloeosporioides penz in mango].	the total dna isolated from mbc-resistant and mbc-sensitive isolates of colletotrichum gloeosporioides penz (c.g.m) of mango were used as templates in pcr amplification using consensus oligo nucleotide primers designed according to the known sequence data of beta-tubulin-encoding gene (tub 1 and tub2) of colletotrichum gloeosporioides f. sp. aeschynomene (c. g. a). only the primers designed according to c. g. a tub2 amplified specific fragments. these amplified fragments were cloned and sequence ...	2004	16110970
cgopt1, a putative oligopeptide transporter from colletotrichum gloeosporioides that is involved in responses to auxin and pathogenicity.	the fungus colletotrichum gloeosporioides f. sp. aeschynomene produces high levels of indole-3-acetic acid (iaa) in axenic cultures and during plant infection. we generated a suppression subtractive hybridization library enriched for iaa-induced genes and identified a clone, which was highly expressed in iaa-containing medium.	2009	19698103
electroporation and agrobacterium-mediated spore transformation.	genetic transformation is a key technology in modern fungal research. most commonly, protoplasts are transformed using the polyethylene glycol-mediated transformation protocols. because protoplasts are generated by treatment of mycelia with a crude enzyme preparation, the results tend to be inconsistent. furthermore, some species cannot be transformed by this method. electroporation (ep) and agrobacterium tumefaciens-mediated transformation (amt) are two alternative methods. these methods allow ...	2010	20238258