Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| sporulation of bacillus sphaericus grown in association with erwinia atroseptica. | 1961 | 16561899 | |
| microbial interactions: population and sporulation studies of bacillus sphaericus grown in association with erwinia atroseptica. | 1964 | 14160080 | |
| [the resistance against drying of pectobacterium carotovorum var. atrosepticum (van hall) dowson (causative agent of tuber rot and blackening of potatoes)]. | 1970 | 4926639 | |
| [salmonella-shigella-agar, a simple medium for isolation of pectobacterium carotovorum var. atrosepticum (van hall) dowson in comparison with other special substrates]. | 1972 | 4562782 | |
| properties of erwinia atroseptica and erwinia carotovora. | 1973 | 4595821 | |
| [the influence of choramphenicol and streptomycinsulphate on the growth of pectobacterium carotovorum var. atrosepticum (van hall) dowson (author's transl)]. | the influence of the antibiotics chloraphenicol (d-threo-form) and streptomycin sulphate against pectobacterium carotovorum var. atrosepticum (syn. erwinia carotovora) was investigated in vitro and on potato tubers. chloramphenicol showed a greater effect as streptomycinsulphate in the applied concentrations. the success of the control of soft rot is determined by the period between the inoculation and the treatment of the tubers. 6 several strains of p. carotovorum var. atrosepticum showed a di ... | 1976 | 1037182 |
| taxonomy of the genus serratia. | one hundred and fifty-six strains of serratia and related bacteria including representatives of enterobacter liquefaciens, enterobacter cloacae, enterobacter aerogenes, erwinia carotovora, erwinia chrysanthemi, erwinia herbicola and erwinia nimipressuralis were studied using 223 morphological, physiological, biochemical and carbon source utilization tests. the results were subjected to computer analysis. at the 80% similarity level all strains, except two, grouped into eight phenons representing ... | 1977 | 319202 |
| serological relationships among flagella of erwinia carotovor var. atroseptica and some e. carotovora var. carotovora serogorups. | the 2 erwinia carotovora var. atroseptica serogroups and 2 out of 16 e. carotovora var. carotovora serogroups previously established on the basis of diffusible somatic antigens were shown to be serologically related by agglutination procedures using whole cells. the common agglutinating antigen in serogroups i, iii, v, and xviii was heat labile and identified as the bacterial flagella by the fluorescent antibody staining procedure. a few strains in serogorups i and iii apparently lacked flagella ... | 1980 | 6156748 |
| comparison of pectic enzymes produced by erwinia chrysanthemi, erwinia carotovora subsp. carotovora, and erwinia carotovora subsp. atroseptica. | erwinia spp. that cause soft-rot diseases in plants produce a variety of extracellular pectic enzymes. to assess the correlation between patterns of pectic enzyme production and taxonomic classification, we compared the enzymes from representative strains. supernatants obtained from polygalacturonate-grown cultures of nine strains of erwinia chrysanthemi, three strains of e. carotovora subsp. carotovora, and three strains of e. carotovora subsp. atroseptica were concentrated and subjected to ult ... | 1986 | 3752996 |
| hemagglutinating activity in phytopathogenic bacteria surface compounds. | extracellular components of plant pathogenic bacteria were obtained from their culture medium as well as from the whole cells by using nacl 1 m, ph 6.0; 20% sucrose dissolved in 0.03 m tris buffer, ph 8.0; or 0.05 m na2edta. all the extracts from erwinia carotovora subsp. carotovora, xanthomonas campestris pv. campestris, pseudomonas syringae pv. phaseolicola, xanthomonas campestris pv. phaseoli, pseudomonas solanacearum, and erwinia carotovora subsp. atroseptica, were assayed for hemagglutinati ... | 1987 | 3625474 |
| isolation and characterisation of transposon-induced mutants of erwinia carotovora subsp. atroseptica exhibiting reduced virulence. | the blackleg pathogen erwinia carotovora subsp. atroseptica (eca) causes an economically important disease of potatoes. we selected a genetically amenable eca strain for the genetic analysis of virulence. tn5 mutagenesis was used to generate nine mutants which exhibited reduced virulence (rvi-) of strain scri1043. following physiological characterisation, mutants were divided into three classes: (1) auxotrophs; (2) extracellular enzyme mutants; and (3) a growth rate mutant. the isolation of thes ... | 1989 | 2549365 |
| proton-linked l-rhamnose transport, and its comparison with l-fucose transport in enterobacteriaceae. | 1. an alkaline ph change occurred when l-rhamnose, l-mannose or l-lyxose was added to l-rhamnose-grown energy-depleted suspensions of strains of escherichia coli. this is diagnostic of sugar-h+ symport activity. 2. l-rhamnose, l-mannose and l-lyxose were inducers of the sugar-h+ symport and of l-[14c]rhamnose transport activity. l-rhamnose also induced the biochemically and genetically distinct l-fucose-h+ symport activity in strains competent for l-rhamnose metabolism. 3. steady-state kinetic m ... | 1993 | 8384447 |
| a pleiotropic reduced virulence (rvi-) mutant of erwinia carotovora subspecies atroseptica is defective in flagella assembly proteins that are conserved in plant and animal bacterial pathogens. | erwinia carotovora subsp. atroseptica was mutagenized and assayed for virulence in planta. those mutants which exhibited reduced virulence (rvi-) were assayed for growth rate, auxotrophy and extracellular enzyme secretion and seven mutants were found to be wild type for all of these phenotypes. when screened for other phenotypes, two were found to be non-motile. one mutant was complemented for motility by a heterologous gene library. a 2.7kb xmaiii-clai complementing fragment was sequenced and t ... | 1993 | 8412685 |
| anaerobic nitrate respiration by erwinia carotovora subsp. atroseptica during potato tuber invasion. | the in planta induction of anaerobic nitrate respiration by erwinia carotovora subsp. atroseptica in relation to the in situ oxygen status in soft rotting potato tubers has been investigated. in vitro experiments have shown that nitrate was required for the induction of respiratory nitrate reductase activity in e. carotovora. in addition, oxygen was found to repress this activity. expression of respiratory nitrate reductase was found in e. carotovora cells extracted from soft rotting potato tube ... | 1993 | 16349082 |
| [expression of pel genes of erwinia chrysanthemi ena49 in erwinia carotovora var. atroseptica 36a cells]. | erwinia atroseptica 36a cells were transformed by the recombinant plasmid ppl5-1 (a derivative of the vector plasmid puc19) containing pelb and pelc genes which encode pectate lyases of erwinia chrysanthemi ena49. synthesis of pectate lyases plb and plc determined by the cloned pel genes is constitutive in erwinia atroseptica 36appl5-1 cells and not inducible by sodium polypectate. the major part of these enzymes was accumulated in the periplasmic fraction of erwinia atroseptica and cells were u ... | 1993 | 8371722 |
| [expression of pectate lyase genes of erwinia carotovora subsp. carotovora 17a and erwinia carotovora subsp. atroseptica 36a in erwinia carotovor substp. atroseptica 36a cells]. | e.atroseptica 36a cells were transformed by the recombinant plasmids p27-1 and pea364 (derivatives of the vector plasmid puc19) containing pectate lyase genes of e.carotovora 17a and e.atroseptica 36a, respectively. the synthesis of pectate lyases determined by the cloned genes of bacteria of both subspecies, as well as the synthesis of the native enzymes, were induced by sodium poly pectate. increase of the dose of pectate lyase genes did not result in alteration of pectate lyase secretion by e ... | 1994 | 7739592 |
| characterization of monoclonal antibodies specific for erwinia carotovora subsp. atroseptica and comparison of serological methods for its sensitive detection on potato tubers. | seven monoclonal antibodies (mabs) to erwinia carotovora subsp. atroseptica have been produced. one, called 4g4, reacted with high specificity for serogroup i of e. carotovora subsp. atroseptica, the most common serogroup on potato tubers in different serological assays. eighty-six strains belonging to different e. carotovora subsp. atroseptica serogroups were assayed. some strains of serogroup xxii also reacted positively. no cross-reactions were observed against other species of plant pathogen ... | 1994 | 16349293 |
| pcr and restriction fragment length polymorphism of a pel gene as a tool to identify erwinia carotovora in relation to potato diseases. | using a sequenced pectate lyase-encoding gene (pel gene), we developed a pcr test for erwinia carotovora. a set of primers allowed the amplification of a 434-bp fragment in e. carotovora strains. among the 89 e. carotovora strains tested, only the erwinia carotovora subsp. betavasculorum strains were not detected. a restriction fragment length polymorphism (rflp) study was undertaken on the amplified fragment with seven endonucleases. the sau3ai digestion pattern specifically identified the erwi ... | 1994 | 7912502 |
| isolation by genomic subtraction of dna probes specific for erwinia carotovora subsp. atroseptica. | erwinia carotovora subsp. atroseptica is a pathogen of potatoes in europe because of its ability to induce blackleg symptoms early in the growing season. however, e. carotovora subsp. carotovora is not able to produce such severe symptoms under the same conditions. on the basis of the technique described by straus and ausubel (proc. natl. acad. sci. usa 87:1889-1893, 1990), we isolated dna sequences of e. carotovora subsp. atroseptica 86.20 that were absent from the genomic dna of e. carotovora ... | 1994 | 8117082 |
| characterization of monoclonal antibodies against erwinia carotovora subsp. atroseptica serogroup i: specificity and epitope analysis. | the characteristics of two monoclonal antibodies (mabs), a23/1221.59.44.d.3 (1221) and a23/1239.36.64.e.2 (1239), against erwinia carotovora subsp. atroseptica serogroup i produced in this study were compared with those of two other independently obtained mabs, 4g4 in spain and 4f6 in canada, using different strains as immunogen and different screening procedures. the reaction pattern of mabs 1221 and 1239 determined by indirect elisa on over 200 bacterial strains including five e.c. atroseptica ... | 1995 | 7538107 |
| [mapping chromosomes of erwinia carotovora subsp. atroseptica 3-2]. | two hfr-like donor strains of bacteria erwinia carotovora subsp. atroseptica (eca) 3-2 were developed by integration into the chromosome of the conjugative plasmid r471a via homology with transposon tn9. using these and two donor strains created earlier, we constructed the genetic map of a fragment of the chromosome of strain eca 3-2. the location of 14 loci is shown in this map. | 1995 | 7590201 |
| [a circular genetic map of chromosomes from erwinia carotovora subsp. atroseptica 3-2]. | a circular genetic map of the bacterium erwinia carotovora subsp. atroseptica 3-2 was constructed on the basis of the r471a plasmid and tn5 and tn9 using hfr-like donors. forty-six genes, including phytopathogenicity genes, were located on the basis of interrupted mating experiment results and analysis of coinheritance of markers on a map of 183 min in length. the similarity and differences of chromosomal genetic maps of erwinia genus bacteria are discussed. | 1995 | 7590213 |
| characterization of pseudomonas aeruginosa flio, a gene involved in flagellar biosynthesis and adherence. | pseudomonas aeruginosa binds to eukaryotic cells via both pilus and nonpilus adhesins, while binding of p. aeruginosa to mucin is pilus independent. to characterize genes involved in non-pilus-mediated adherence, transposon mutants of the nonpiliated strain p. aeruginosa pak-np that are unable to bind to cells or mucins were isolated. two such mutants, p. aeruginosa b164 and p. aeruginosa rr18, were identified previously as deficient in binding to eukaryotic cells or mucins as well as nonmotile. ... | 1995 | 7622217 |
| synergism between erwinia pectate lyase isoenzymes that depolymerize both pectate and pectin. | phytopathogenic erwinia bacteria cause tissue maceration by secretion of pectinolytic enzymes such as pectate lyase (pl). sequencing of overlapping genomic fragments from erwinia carotovora subsp. atroseptica established the organization of a 7.5 kbp region encoding pl isoenzymes. two intergenic regions of 656 and 645 bp separate three enzyme coding regions of 1125 bp exhibiting approximately 80% positional identity. the promoters of each of the three genes contain a segment with high homology t ... | 1995 | 7773390 |
| expression of an erwinia pectate lyase in three species of aspergillus. | transgenic filamentous fungi of the species aspergillus niger, a. nidulans and a. awamori expressing and secreting erwinia carotovora subsp. atroseptica pectate lyase 3 (pl3) were generated. correct processing of the pre-enzyme was achieved using the a. niger pectin lyase a (pel a) signal peptide. with the prepro-peptide of a. niger polygalacturonase ii, secreted enzymes still possessed the 6- aa pro-sequence, indicating the importance of the conformation of the precursor protein for correct cle ... | 1996 | 8625428 |
| regulation of pelz, a gene of the pelb-pelc cluster encoding a new pectate lyase of erwinia chrysanthemi 3937. | the phytopathogenic enterobacterium erwinia chrysanthemi 3937 produces five major and several secondary endo-pectate lyases encoded by the pel genes. most of these genes are arranged in clusters on the bacterial chromosome. the genomic region surrounding the pelb-pelc cluster was supposed to be involved in the regulation of pelb and pelc synthesis. we demonstrated that the variation of pelb expression resulted from the titration of a regulatory protein by the gene adjacent to pelc. this gene was ... | 1996 | 8955401 |
| immunomagnetic separation of erwinia carotovora subsp. atroseptica from potato peel extracts to improve detection sensitivity on a crystal violet pectate medium or by pcr. | immunomagnetic separation (ims) procedures for the selective separation of erwinia carotovora subsp. atroseptica from potato peel extract were optimized for the recovery of target and removal of non-target bacteria. a streptomycin-resistant strain of erw. carotovora subsp. atroseptica was used in combination with a crystal violet pectate (cvp) medium supplemented with 100 micrograms ml-1 of streptomycin to determine the recovery level of the target bacterium. recovery obtained with a polyclonal ... | 1996 | 9072520 |
| bacillus subtilis bs 107 as an antagonist of potato blackleg and soft rot bacteria. | antimicrobial substances were produced by bacillus subtilis bs 107 in a defined medium and isolated from culture filtrate by precipitation at ph 2.5. active fractions were extracted in ethyl acetate, acetone, and 80% ethanol and purified by thin-layer chromatography (tlc) on silica gel plates developed with an ethanol-water mixture (2:1, v/v). in each case, a band with a rf of 0.75 formed an inhibitory zone when the tlc plates were placed in contact with agar seeded with test cultures of the erw ... | 1998 | 9830107 |
| mutation in a gene required for lipopolysaccharide and enterobacterial common antigen biosynthesis affects virulence in the plant pathogen erwinia carotovora subsp. atroseptica. | spontaneous bacteriophage-resistant mutants of the phytopathogen erwinia carotovora subsp. atroseptica (eca) scri1043 were isolated and, out of 40, two were found to exhibit reduced virulence in planta. one of these mutants, a5/22, showed multiple cell surface defects including alterations in synthesis of outer membrane proteins, lipopolysaccharide (lps), enterobacterial common antigen (eca), and flagella. mutant a5/22 also showed reduced synthesis of the exoenzymes pectate lyase (pel) and cellu ... | 1999 | 10356801 |
| erwinia carotovora dsba mutants: evidence for a periplasmic-stress signal transduction system affecting transcription of genes encoding secreted proteins. | the dsba genes, which encode major periplasmic disulfide-bond-forming proteins, were isolated from erwinia carotovora subsp. carotovora (ecc) and erwinia carotovora subsp. atroseptica (eca), and the dsbc gene, encoding another periplasmic disulfide oxidoreductase was isolated from ecc. all three genes were sequenced and mutants deficient in these genes were created by marker exchange mutagenesis. the ecc mutants were severely affected in activity and secretion of pectate lyase, probably due to t ... | 1999 | 10463161 |
| cdna-aflp analysis of differential gene expression in the prokaryotic plant pathogen erwinia carotovora. | for studies of differential gene expression in prokaryotes, methods for synthesizing representative cdna populations are required. here, a technique is described for the synthesis of cdna from the potato pathogens erwinia carotovora subsp. atroseptica (eca) and erwinia carotovora subsp. carotovora (ecc) using a combination of short oligonucleotide (11-mer) primers that were known to anneal to conserved sequences in the 3' regions of enterobacterial genes. specific pcr amplifications with primers ... | 2000 | 10658663 |
| a competitive pcr-based method for the detection and quantification of erwinia carotovora subsp. atroseptica on potato tubers. | a pcr-based method was developed for the simultaneous detection and quantification of the potato pathogen erwinia carotovora subsp. atroseptica (eca) on potato tubers. the method incorporates a competitor pcr template cloned into escherichia coli in vector pgem-t (e. coli 4r l/l). predetermined numbers of e. coli 4r were added to potato peel extract, either pre-inoculated with eca or from naturally contaminated tubers, and eca numbers estimated by comparing the ratio of products generated from e ... | 2000 | 10792657 |
| a potato gene encoding a wrky-like transcription factor is induced in interactions with erwinia carotovora subsp. atroseptica and phytophthora infestans and is coregulated with class i endochitinase expression. | a potato gene encoding a putative wrky protein was isolated from a cdna library enriched by suppression subtractive hybridization for sequences upregulated 1 h postinoculation with erwinia carotovora subsp. atroseptica. the cdna encodes a putative polypeptide of 172 amino acids, containing a single wrky domain with a zinc finger motif and preceded by a potential nuclear localization site. st-wrky1 was strongly upregulated in compatible, but only weakly in incompatible, interactions with phytopht ... | 2000 | 11043470 |
| hexa of erwinia carotovora ssp. carotovora strain ecc71 negatively regulates production of rpos and rsmb rna, a global regulator of extracellular proteins, plant virulence and the quorum-sensing signal, n-(3-oxohexanoyl)-l-homoserine lactone. | the soft-rotting bacterium, erwinia carotovora ssp. carotovora (e. c. carotovora), produces an array of extracellular enzymes (= exoenzymes), including pectate lyase (pel), polygalacturonase (peh), cellulase (cel) and protease (prt), as well as harpinecc, the elicitor of hypersensitive reaction (hr). the production of these exoenzymes and harpinecc responds to plant products and the quorum-sensing signal [n-(3-oxohexanoyl)-l-homoserine lactone; ohl] and is subject to both transcriptional and pos ... | 2000 | 11220306 |
| [characteristics of bacterial lipopolysaccharides depending on extraction method]. | carbohydrate-containing biopolymers have been isolated from erwinia carotovora subsp. atroseptica 549 by two methods--the aqueous-phenol and with using physiological solution--with addition and without addition of ethylenediaminetetraacetate (edta). the biopolymers yield from the cells of bacteria are shown to depend on the extraction method. lipopolysaccharide-protein complex have been isolated by the sparing method. the purest lipopolysaccharide have been isolated by the aqueousphenol method. ... | 2000 | 11392785 |
| rapid identification and differentiation of the soft rot erwinias by 16s-23s intergenic transcribed spacer-pcr and restriction fragment length polymorphism analyses. | current identification methods for the soft rot erwinias are both imprecise and time-consuming. we have used the 16s-23s rrna intergenic transcribed spacer (its) to aid in their identification. analysis by its-pcr and its-restriction fragment length polymorphism was found to be a simple, precise, and rapid method compared to current molecular and phenotypic techniques. the its was amplified from erwinia and other genera using universal pcr primers. after pcr, the banding patterns generated allow ... | 2001 | 11526007 |
| genotyping of bacteria belonging to the former erwinia genus by pcr-rflp analysis of a reca gene fragment. | genotypic characterization, based on the analysis of restriction fragment length polymorphism of the reca gene fragment pcr product (reca pcr-rflp), was performed on members of the former erwinia genus. pcr primers deduced from published reca gene sequences of erwinia carotovora allowed the amplification of an approximately 730 bp dna fragment from each of the 19 erwinia species tested. amplified reca fragments were compared using rflp analysis with four endonucleases (alui, hinfi, tasi and tru1 ... | 2002 | 11832521 |
| application of amplified fragment length polymorphism fingerprinting for taxonomy and identification of the soft rot bacteria erwinia carotovora and erwinia chrysanthemi. | the soft rot bacteria erwinia carotovora and erwinia chrysanthemi are important pathogens of potato and other crops. however, the taxonomy of these pathogens, particularly at subspecies level, is unclear. an investigation using amplified fragment length polymorphism (aflp) fingerprinting was undertaken to determine the taxonomic relationships within this group based on their genetic relatedness. following cluster analysis on the similarity matrices derived from the aflp gels, four clusters (clus ... | 2002 | 11916661 |
| [study of population dissociation of collection strains of erwinia carotovora]. | unusual dissociants of erwinia carotovora subsp. atroseptica (eat) population that emerge with high frequency (18-94%) during long-term storage of cultures were found. the dissociants (poe-phenotype) differ from parental forms by slow growth and long development of the colour of colonies on emb agar though they are not lac-mutants. more than 60% of poe-mutants of strain eat 39a are characterized by 8- and 150-fold decrease of stability to erythromycin and oleandomicin, correspondingly. they are ... | 2002 | 11944340 |
| response of endophytic bacterial communities in potato plants to infection with erwinia carotovora subsp. atroseptica. | the term endophyte refers to interior colonization of plants by microorganisms that do not have pathogenic effects on their hosts, and various endophytes have been found to play important roles in plant vitality. in this study, cultivation-independent terminal restriction fragment length polymorphism analysis of 16s ribosomal dna directly amplified from plant tissue dna was used in combination with molecular characterization of isolates to examine the influence of plant stress, achieved by infec ... | 2002 | 11976096 |
| sample sequencing of a selected region of the genome of erwinia carotovora subsp. atroseptica reveals candidate phytopathogenicity genes and allows comparison with escherichia coli. | genome sequencing is making a profound impact on microbiology. currently, however, only one plant pathogen genome sequence is publicly available and no genome-sequencing project has been initiated for any species of the genus erwinia, which includes several important plant pathogens. this paper describes a targeted sample sequencing approach to study the genome of erwinia carotovora subsp. atroseptica (eca), a major soft-rot pathogen of potato. a large insert dna (approx. 115 kb) library of eca ... | 2002 | 11988510 |
| inhibition of the plastidic atp/adp transporter protein primes potato tubers for augmented elicitation of defense responses and enhances their resistance against erwinia carotovora. | tubers of transgenic potato (solanum tuberosum) plants with decreased activity of the plastidic atp/adp transporter aatp1 display reduced levels of starch, modified tuber morphology, and altered concentrations of primary metabolites. here, we demonstrate that the spontaneous production of hydrogen peroxide, the endogenous content of salicylic acid, and the levels of mrnas of various defense-related genes are similar in tuber discs of wild-type and aatp1(st) antisense plants. however, upon challe ... | 2002 | 12177473 |
| elevation of three subspecies of pectobacterium carotovorum to species level: pectobacterium atrosepticum sp. nov., pectobacterium betavasculorum sp. nov. and pectobacterium wasabiae sp. nov. | a collection of 42 strains belonging to the five subspecies of pectobacterium carotovorum (subspecies atrosepticum, betavasculorum, carotovorum, odoriferum and wasabiae) and 11 reference and type strains of biovars of pectobacterium chrysanthemi, pectobacterium cacticidum and brenneria paradisiaca were studied by dna-dna hybridization, numerical taxonomy of 120 phenotypic characteristics, serology and new phylogenetic analysis of previously reported sequences from a database of aligned 16s rdna ... | 2003 | 12710602 |
| an oligonucleotide array for the identification and differentiation of bacteria pathogenic on potato. | abstract oligonucleotides, 16 to 24 bases long, were selected from the 3' end of the 16s gene and the 16s-23s intergenic spacer regions of bacteria pathogenic on potato, including clavibacter michiganensis subsp. sepedonicus, ralstonia solanacearum, and the pectolytic erwinias, including erwinia carotovora subsp. atroseptica and carotovora and e. chrysanthemi. oligonucleotides were designed and formatted into an array by pin spotting on nylon membranes. genomic dna from bacterial cultures was am ... | 2003 | 18944335 |
| thermodependence of growth and enzymatic activities implicated in pathogenicity of two erwinia carotovora subspecies (pectobacterium spp.). | erwinia carotovora subsp. atroseptica and erwinia carotovora subsp. carotovora can cause substantial damage to economically important plant crops and stored products. the occurrence of the disease and the scale of the damage are temperature dependent. disease development consists first of active multiplication of the bacteria in the infection area and then production of numerous extracellular enzymes. we investigated the effects of various temperatures on these two steps. we assayed the specific ... | 2004 | 15052318 |
| genome sequence of the enterobacterial phytopathogen erwinia carotovora subsp. atroseptica and characterization of virulence factors. | the bacterial family enterobacteriaceae is notable for its well studied human pathogens, including salmonella, yersinia, shigella, and escherichia spp. however, it also contains several plant pathogens. we report the genome sequence of a plant pathogenic enterobacterium, erwinia carotovora subsp. atroseptica (eca) strain scri1043, the causative agent of soft rot and blackleg potato diseases. approximately 33% of eca genes are not shared with sequenced enterobacterial human pathogens, including s ... | 2004 | 15263089 |
| use of a pooled transposon mutation grid to demonstrate roles in disease development for erwinia carotovora subsp. atroseptica putative type iii secreted effector (dspe/a) and helper (hrpn) proteins. | soft rot erwinia spp., like other closely related plant pathogens, possess a type iii secretion system (ttss) (encoded by the hrp gene cluster) implicated in disease development. we report the sequence of the entire hrp gene cluster and adjacent dsp genes in erwinia carotovora subsp. atroseptica scri1039. the cluster is similar in content and structural organization to that in e. amylovora. however, eight putative genes of unknown function located within the e. carotovora subsp. atroseptica clus ... | 2004 | 15384484 |
| identification of an antibacterial compound, benzylideneacetone, from xenorhabdus nematophila against major plant-pathogenic bacteria. | an entomopathogenic bacterium, xenorhabdus nematophila, is known to have potent antibiotic activities to maintain monoxenic condition in its insect host for effective pathogenesis and ultimately for optimal development of its nematode symbiont, steinernema carpocapsae. in this study we assess its antibacterial activity against plant-pathogenic bacteria and identify its unknown antibiotics. the bacterial culture broth had significant antibacterial activity that increased with development of the b ... | 2004 | 15476972 |
| ultrastructural alterations of erwinia carotovora subsp. atroseptica caused by treatment with aluminum chloride and sodium metabisulfite. | aluminum and bisulfite salts inhibit the growth of several fungi and bacteria, and their application effectively controls potato soft rot caused by erwinia carotovora. in an effort to understand their inhibitory action, ultrastructural changes in erwinia carotovora subsp. atroseptica after exposure (0 to 20 min) to different concentrations (0.05, 0.1, and 0.2 m) of these salts were examined by using transmission electron microscopy. plasma membrane integrity was evaluated by using the sytox gree ... | 2004 | 15528547 |
| involvement of n-acylhomoserine lactones throughout plant infection by erwinia carotovora subsp. atroseptica (pectobacterium atrosepticum). | erwinia carotovora subsp. atroseptica is responsible for potato blackleg disease in the field and tuber soft rot during crop storage. the process leading to the disease occurs in two phases: a primary invasion step followed by a maceration step. bacteria-to-bacteria communication is associated with a quorum-sensing (qs) process based on the production of n-acylhomoserine lactones (hsl). the role of hsl throughout plant infection was analyzed. to this purpose, hsl produced by a specific e. caroto ... | 2004 | 15553252 |
| [genetic regulation of pathogenicity and virulence factors in bacteria erwinia carotovora subsp. atroseptica: phenotypic characteristic of bacteria with the mutant kdud gene]. | in contrast to the closely related bacteria erwinia chrysanthemi, bacteria erwinia carotovora subsp. atroseptica produce lower levels of main pathogenicity and virulence factors (pectate lyases, cellulases, and proteases) in the presence of pectins. this effect was shown to be connected with the accumulation of the intermediate product of intracellular degradation of these substances, 2,5-diketo-3-deoxygluconate (dk2). the presence of dk2 in the culture broth of mutant bacteria, connected to its ... | 2004 | 15559146 |
| [genetic regulation of pathogenicity and virulence factors in bacteria erwinia carotovora subsp. atroseptica: identification of kdud gene]. | a mutant that cannot utilize pectin substances of plant cell walls was obtained via insertion of mini-mini-tn5xyle transposon into the chromosome of phytopathogenic bacteria erwinia carotovora subsp. atroseptica. the inability of mutant cells to utilize these substrates was caused by a failure to accomplish the catabolism of unsaturated digalacturonic acid (uda). study of enzymatic activities has established that mutant bacteria lost the ability to produce 2,5-diketo-3-deoxygluconate dehydrogena ... | 2004 | 15559145 |
| the 120 592 bp incf plasmid prsb107 isolated from a sewage-treatment plant encodes nine different antibiotic-resistance determinants, two iron-acquisition systems and other putative virulence-associated functions. | the antibiotic-multiresistance incf plasmid prsb107 was isolated by a transformation-based approach from activated-sludge bacteria of a wastewater-treatment plant. it confers resistance to ampicillin, penicillin g, chloramphenicol, erythromycin, kanamycin, neomycin, streptomycin, sulfonamides, tetracycline and trimethoprim and against mercuric ions. complete sequencing of this plasmid revealed that it is 120 592 bp in size and has a g+c content of 53.1 mol%. the plasmid backbone is composed of t ... | 2005 | 15817778 |
| identification of a new quorum-sensing-controlled virulence factor in erwinia carotovora subsp. atroseptica secreted via the type ii targeting pathway. | two-dimensional polyacrylamide gel electrophoresis of the secreted proteins of erwinia carotovora subsp. atroseptica revealed a low-abundance protein that was identified by mass spectrometry as a homologue of a xanthomonas campestris avirulence protein with unknown function. the predicted svx protein has an n-terminal signal sequence and zinc binding-region signature, and the mature protein is post-translationally modified. a 2d difference gel electrophoresis (dige) showed that the protein is se ... | 2005 | 15828685 |
| lethal effect of the gliding arc discharges on erwinia spp. | to compare the decontamination performances of glidarc on strains of erwinia of industrial interest. | 2005 | 15836472 |
| elevated temperature enhances virulence of erwinia carotovora subsp. carotovora strain ec153 to plants and stimulates production of the quorum sensing signal, n-acyl homoserine lactone, and extracellular proteins. | erwinia carotovora subsp. atroseptica, e. carotovora subsp. betavasculorum, and e. carotovora subsp. carotovora produce high levels of extracellular enzymes, such as pectate lyase (pel), polygalacturonase (peh), cellulase (cel), and protease (prt), and the quorum-sensing signal n-acyl-homoserine lactone (ahl) at 28 degrees c. however, the production of these enzymes and ahl by these bacteria is severely inhibited during growth at elevated temperatures (31.2 degrees c for e. carotovora subsp. atr ... | 2005 | 16085860 |
| potato homologs of arabidopsis thaliana genes functional in defense signaling--identification, genetic mapping, and molecular cloning. | defense against pests and pathogens is a fundamental process controlled by similar molecular mechanisms in all flowering plants. using arabidopsis thaliana as a model, steps of the signal transduction pathways that link pathogen recognition to defense activation have been identified and corresponding genes have been characterized. defense signaling (ds) genes are functional candidates for controlling natural quantitative variation of resistance to plant pathogens. nineteen arabidopsis genes oper ... | 2005 | 16255250 |
| comparative genomics reveals what makes an enterobacterial plant pathogen. | the bacterial family enterobacteriaceae contains some of the most devastating human and animal pathogens, including escherichia coli, salmonella enterica and species of yersinia and shigella. these are among the best-studied of any organisms, yet there is much to be learned about the nature and evolution of interactions with their hosts and with the wider environment. comparative and functional genomics have fundamentally improved our understanding of their modes of adaptation to different ecolo ... | 2006 | 16704357 |
| lipopolysaccharides of pectobacterium atrosepticum and pseudomonas corrugata induce different defence response patterns in tobacco, tomato, and potato. | lipopolysaccharides (lps), ubiquitous cell surface components of gram-negative bacteria, are directly implicated in plant/pathogen interactions. however, their perception by the plant, the subsequent signal transduction in both compatible and incompatible interactions, as well as the defence reactions induced in compatible interactions are as yet poorly understood. we focused on biochemical and physiological reactions induced in cell suspensions of three solanaceae species (tobacco, tomato, and ... | 2006 | 16755465 |
| the phytopathogen dickeya dadantii (erwinia chrysanthemi 3937) is a pathogen of the pea aphid. | dickeya dadantii (erwinia chrysanthemi) is a phytopathogenic bacterium causing soft rot diseases on many crops. the sequencing of its genome identified four genes encoding homologues of the cyt family of insecticidal toxins from bacillus thuringiensis, which are not present in the close relative pectobacterium carotovorum subsp. atrosepticum. the pathogenicity of d. dadantii was tested on the pea aphid acyrthosiphon pisum, and the bacterium was shown to be highly virulent for this insect, either ... | 2006 | 16517643 |
| thermoregulation of n-acyl homoserine lactone-based quorum sensing in the soft rot bacterium pectobacterium atrosepticum. | the psychrotolerant bacterium pectobacterium atrosepticum produces four n-acyl homoserine lactones under a wide range of temperatures. their thermoregulation differs from that of the exoenzyme production, described as being under quorum-sensing control. a mechanism involved in this thermoregulation consists of controlling n-acyl homoserine lactones synthase production at a transcriptional level. | 2007 | 17468275 |
| growth promotion of quorum-quenching bacteria in the rhizosphere of solanum tuberosum. | among 17 molecules structurally related to n-acylhomoserine lactone (nahl), gamma-caprolactone (gcl), 6-caprolactone (6cl) and 4-heptanolide (htn) were found to stimulate the degradation of nahl by bacterial communities recovered from bulk and rhizospheric soils. in the 6cl-, gcl- and htn-treated bacterial consortia, the nahl-degrading bacteria were more abundant than in control (mannitol-treated) consortia. moreover, the gcl- and htn-consortia showed a biocontrol activity against pectobacterium ... | 2007 | 17504488 |
| gliding arc discharge in the potato pathogen erwinia carotovora subsp. atroseptica: mechanism of lethal action and effect on membrane-associated molecules. | gliding arc (glidarc) discharge is a physicochemical technique for decontamination at atmospheric pressure and ambient temperature. it leads to the destruction of bacterial phytopathogens responsible for important losses in industrial agriculture, namely, erwinia spp., without the formation of resistant forms. we investigated the effect of a novel optimized prototype allowing bacterial killing without lag time. this prototype also decreases the required duration of treatment by 50%. the study of ... | 2007 | 17644644 |
| host-extract induced changes in the secretome of the plant pathogenic bacterium pectobacterium atrosepticum. | pectobacterium atrosepticum is a gram-negative plant pathogenic bacterium that causes rotting in potato stems and tubers. the secreted proteins of this pathogen were analyzed with proteomics from culture supernatant of cells grown in minimal medium supplemented with host extracts. more than 40 proteins were identified, among them known virulence determinants, such as pectic enzymes, metalloprotease, and virulence protein svx, along with flagella proteins, groel and cyclophilin-type chaperones an ... | 2007 | 17726675 |
| rela-dependent (p)ppgpp production controls exoenzyme synthesis in erwinia carotovora subsp. atroseptica. | in this report, we investigate the link between nutrient limitation, rela-mediated (p)ppgpp production, and virulence in the phytopathogen erwinia carotovora subsp. atroseptica. a rela null mutant (jwc7) was constructed by allelic exchange, and we confirmed that, unlike the wild-type progenitor, this mutant did not produce elevated levels of (p)ppgpp upon nutrient downshift. however, (p)ppgpp production could be restored in strain jwc7 during nutrient limitation by supplying rela in trans. durin ... | 2007 | 17766416 |
| evaluation of reference genes for real-time rt-pcr expression studies in the plant pathogen pectobacterium atrosepticum. | real-time rt-pcr has become a powerful technique to monitor low-abundance mrna expression and is a useful tool when examining bacterial gene expression inside infected host tissues. however, correct evaluation of data requires accurate and reliable normalisation against internal standards. thus, the identification of reference genes whose expression does not change during the course of the experiment is of paramount importance. here, we present a study where manipulation of cultural growth condi ... | 2007 | 17888160 |
| effective biotic elicitation of ruta graveolens l. shoot cultures by lysates from pectobacterium atrosepticum and bacillus sp. | growth of ruta graveolens shoots was induced when bacillus sp. cell lysates were added to the culture medium. elicitation of coumarin by this lysate was also very effective; the concentrations of isopimpinelin, xanthotoxin and bergapten increased to 610, 2120 and 1460 microg g(-1) dry wt, respectively. it also had a significant effect on the production of psoralen and rutamarin (680 and 380 microg g(-1) dry wt) and induced the biosynthesis of chalepin, which was not detected in the control sampl ... | 2008 | 17968510 |
| citrate uptake into pectobacterium atrosepticum is critical for bacterial virulence. | to analyze whether metabolite import into pectobacterium atrosepticum cells affects bacterial virulence, we investigated the function of a carrier which exhibits significant structural homology to characterized carboxylic-acid transport proteins. the corresponding gene, eca3984, previously annotated as coding for a na(+)/sulphate carrier, in fact encodes a highly specific citrate transporter (cit1) which is energized by the proton-motive force. expression of the cit1 gene is stimulated by the pr ... | 2008 | 18393614 |
| the genome of erwinia tasmaniensis strain et1/99, a non-pathogenic bacterium in the genus erwinia. | the complete genome of the bacterium erwinia tasmaniensis strain et1/99 consisting of a 3.9 mb circular chromosome and five plasmids was sequenced. strain et1/99 represents an epiphytic plant bacterium related to erwinia amylovora and e. pyrifoliae, which are responsible for the important plant diseases fire blight and asian pear shoot blight, respectively. strain et1/99 is a non-pathogenic bacterium and is thought to compete with these and other bacteria when occupying the same habitat during i ... | 2008 | 18462403 |
| quorum sensing coordinates brute force and stealth modes of infection in the plant pathogen pectobacterium atrosepticum. | quorum sensing (qs) in vitro controls production of plant cell wall degrading enzymes (pcwdes) and other virulence factors in the soft rotting enterobacterial plant pathogen pectobacterium atrosepticum (pba). here, we demonstrate the genome-wide regulatory role of qs in vivo during the pba-potato interaction, using a pba-specific microarray. we show that 26% of the pba genome exhibited differential transcription in a qs (expi-) mutant, compared to the wild-type, suggesting that qs may make a gre ... | 2008 | 18566662 |
| microarray profiling of host-extract-induced genes and characterization of the type vi secretion cluster in the potato pathogen pectobacterium atrosepticum. | pectobacterium atrosepticum is a gram-negative plant-pathogenic bacterium that rots potato stems and tubers. microarray analysis was used to identify genes that were differentially expressed when host extracts were added to the growth medium. potato extracts downregulated the expression of ribosomal genes and genes related to uptake and metabolism of nutrients, and upregulated genes needed for nitrate or phosphonate use. some of the observed changes in gene expression in host-extract-induced cul ... | 2008 | 18667571 |
| a culture filtrate of phytophthora infestans primes defense reaction in potato cell suspensions. | priming of defense reactions by an elicitor results in an enhanced ability of the plant to respond to subsequent pathogen challenges. we previously showed that application of lipopolysaccharides (lps) to potato cell suspensions causes apoplastic acidification, but does not stimulate lipoxygenase (lox) activity. here, we tested the ability of various elicitors to prime and elicit defense reactions in potato cell suspensions. adding 20 microg ml(1) lps, laminarin, harpin n, or a concentrated cultu ... | 2008 | 18944288 |
| distinguishing bacterial pathogens of potato using a genome-wide microarray approach. | a set of 9676 probes was designed for the most harmful bacterial pathogens of potato and tested in a microarray format. gene-specific probes could be designed for all genes of pectobacterium atrosepticum, c. 50% of the genes of streptomyces scabies and c. 30% of the genes of clavibacter michiganensis ssp. sepedonicus utilizing the whole-genome sequence information available. for streptomyces turgidiscabies, 226 probes were designed according to the sequences of a pathogenicity island containing ... | 2008 | 19018999 |
| niche-specificity and the variable fraction of the pectobacterium pan-genome. | we compare genome sequences of three closely related soft-rot pathogens that vary in host range and geographical distribution to identify genetic differences that could account for lifestyle differences. the isolates compared, pectobacterium atrosepticum scri1043, p. carotovorum wpp14, and p. brasiliensis 1692, represent diverse lineages of the genus. p. carotovorum and p. brasiliensis genome contigs, generated by 454 pyrosequencing ordered by reference to the previously published complete circu ... | 2008 | 18986251 |
| dsba plays a critical and multifaceted role in the production of secreted virulence factors by the phytopathogen erwinia carotovora subsp. atroseptica. | erwinia carotovora subsp. atroseptica is an enterobacterial phytopathogen causing economically significant soft rot disease. pathogenesis is mediated by multiple secreted virulence factors, many of which are secreted by the type ii (out) secretion system. dsba catalyzes the introduction of disulfide bonds into periplasmic and secreted proteins. in this study, the extracellular proteome (secretome) of wild type e. carotovora subsp. atroseptica scri1043, and dsba and out mutants, was analyzed by s ... | 2008 | 18562317 |
| the response regulator hrpy of dickeya dadantii 3937 regulates virulence genes not linked to the hrp cluster. | hrpx/y is a putative two-component system (tcs) encoded within the type iii secretion system (t3ss) gene cluster of dickeya dadantii. a linear regulatory cascade initiated by hrpx/y that leads to activation of the downstream t3ss genes via hrps and hrpl was described previously. therefore, in d. dadantii, hrpx/y plays an important role in regulation of genes involved in bacteria-plant interactions and bacterial aggregation via the t3ss. hrpx/y is the only tcs shared among the plant-pathogenic en ... | 2008 | 18257680 |
| reannotation of hypothetical orfs in plant pathogen erwinia carotovora subsp. atroseptica scri1043. | over-annotation of hypothetical orfs is a common phenomenon in bacterial genomes, which necessitates confirming the coding reliability of hypothetical orfs and then predicting their functions. the important plant pathogen erwinia carotovora subsp. atroseptica scri1043 (eca1043) is a typical case because more than a quarter of its annotated orfs are hypothetical. our analysis focuses on annotation of eca1043 hypothetical orfs, and comprises two efforts: (a) based on the z-curve method, 49 origina ... | 2008 | 18067578 |
| the r1162 mob proteins can promote conjugative transfer from cryptic origins in the bacterial chromosome. | the mobilization proteins of the broad-host-range plasmid r1162 can initiate conjugative transfer of a plasmid from a 19-bp locus that is partially degenerate in sequence. such loci are likely to appear by chance in the bacterial chromosome and could act as cryptic sites for transfer of chromosomal dna when r1162 is present. the r1162-dependent transfer of chromosomal dna, initiated from one such potential site in pectobacterium atrosepticum, is shown here. a second active site was identified in ... | 2009 | 19074386 |
| physicochemical basis for the inhibitory effects of organic and inorganic salts on the growth of pectobacterium carotovorum subsp. carotovorum and pectobacterium atrosepticum. | twenty-one salts were tested for their effects on the growth of pectobacterium carotovorum subsp. carotovorum and pectobacterium atrosepticum. in liquid medium, 11 salts (0.2 m) exhibited strong inhibition of bacterial growth. the inhibitory action of salts relates to the water-ionizing capacity and the lipophilicity of their constituent ions. | 2009 | 19114504 |
| lines of evidence for horizontal gene transfer of a phenazine producing operon into multiple bacterial species. | phenazines are secondary metabolites with broad-spectrum antibiotic activity against bacteria, fungi, and eukaryotes. in pseudomonad species, a conserved seven-gene phenazine operon (phzabcdefg) is required for the conversion of chorismic acid to the broad-spectrum antibiotic phenazine-1-carboxylate. previous analyses of genes involved in phenazine production from nonpseudomonad species uncovered a high degree of sequence similarity to pseudomonad homologues. the analyses undertaken in this stud ... | 2009 | 19189039 |
| small rna identification in enterobacteriaceae using synteny and genomic backbone retention ii. | small rnas are bacterial counterparts of noncoding rnas. increasing evidence being added in the literature indicates that these small rnas play major roles in prokaryotes both at the transcriptome and proteome levels. based on comparative genomic studies, we present manually curated small rna regions in 25 recently completed genomes from enterobacteriaceae. the study is a continuation of our earlier work that uses the presence of small rnas sandwiched between specific conserved flanking genes re ... | 2009 | 19445646 |
| mutagenesis and functional characterization of the rna and protein components of the toxin abortive infection and toxin-antitoxin locus of erwinia. | bacteria are constantly challenged by bacteriophage (phage) infection and have developed multiple adaptive resistance mechanisms. these mechanisms include the abortive infection systems, which promote "altruistic suicide" of an infected cell, protecting the clonal population. a cryptic plasmid of erwinia carotovora subsp. atroseptica, peca1039, has been shown to encode an abortive infection system. this highly effective system is active across multiple genera of gram-negative bacteria and agains ... | 2009 | 19633081 |
| microarray comparative genomic hybridisation analysis incorporating genomic organisation, and application to enterobacterial plant pathogens. | microarray comparative genomic hybridisation (acgh) provides an estimate of the relative abundance of genomic dna (gdna) taken from comparator and reference organisms by hybridisation to a microarray containing probes that represent sequences from the reference organism. the experimental method is used in a number of biological applications, including the detection of human chromosomal aberrations, and in comparative genomic analysis of bacterial strains, but optimisation of the analysis is desi ... | 2009 | 19696881 |
| specific maceration and induction of pr-3 gene in potato tuber tissue by pectobacterium carotovorum subsp. atrosepticum type iii secretion system mutants. | the exact function of type iii secretion system in some phytopathogenes including pectobacterium carotovorum subsp. atrosepticum (pca) is not understood and is a matter of debate. the aim of this study were to determine specific effect of type iii secretion system on potato tubers and to reveal the connection of this system with potato resistant genes such as pr-3. a pca hrpw fragment was subcloned into a low-copy-number cloning vector (pzh448). the resulting plasmid (pas19) was then conjugated ... | 2009 | 20334119 |
| comparison of specificity and sensitivity of immunochemical and molecular techniques for determination of clavibacter michiganensis subsp. michiganensis. | detection of clavibacter michiganensis subsp. michiganensis (cmm), causing bacterial canker of tomato, was verified using pta-elisa and ifas with pabs of neogen europe ltd. (uk), and with published and also laboratory-generated pcr primers from the cmm tomatinase gene. the specificity of this technique was determined with 15 plant-pathogenic and 4 common, saprophytic bacteria. with ifas, crossreactions were found for pantoea dispersa, p. agglomerans and rahnella aquatilis, and with pta-elisa for ... | 2010 | 20526836 |
| reverse engineering gene regulatory networks related to quorum sensing in the plant pathogen pectobacterium atrosepticum. | the objective of the project reported in the present chapter was the reverse engineering of gene regulatory networks related to quorum sensing in the plant pathogen pectobacterium atrosepticum from micorarray gene expression profiles, obtained from the wild-type and eight knockout strains. to this end, we have applied various recent methods from multivariate statistics and machine learning: graphical gaussian models, sparse bayesian regression, lasso (least absolute shrinkage and selection opera ... | 2010 | 20835805 |
| occurrence of pectobacterium carotovorum strains isolated from potato soft rot in morocco. | pectobacterium carotovorum subsp. carotovorum, pectobacterium astrosepticum and pectobacterium chrysanthemi are the soft rot tuber of potatoes pathogens (solanum tuberosum). the aim of this study was to determine the occurrence of these pathogens in moroccan regions producing potatoes. fifty three isolates of pectobacterium were isolated on medium crystal violet pectate. the comparison of their bacteriological characteristics with standard strains allowed us to conclude that all the isolates bel ... | 2010 | 20937219 |
| effect of water activity on the production of volatile organic compounds by muscodor albus and their effect on three pathogens in stored potato. | muscodor albus (xylariaceae, ascomycetes) isolate cz-620 produces antimicrobial volatile organic compounds (voc), which appear to have potential for the control of various postharvest diseases. the effect of water activity (aw) on the production of voc by m. albus culture, and their inhibitory effects on the growth of three pathogens of potato tuber (fusarium sambucinum, helminthosporium solani, and pectobacterium atrosepticum) and the development of diseases caused by the three pathogens (dry r ... | 2010 | 21354528 |
| phage-selected lipopolysaccharide mutants of pectobacterium atrosepticum exhibit different impacts on virulence. | to positively select pectobacterium atrosepticum (pa) mutants with cell surface defects and to assess the impact of these mutations on phytopathogenesis. | 2010 | 20132374 |
| two mobile pectobacterium atrosepticum prophages modulate virulence. | the pectobacterium atrosepticum strain scri1043 genome contains two complete prophage sequences. one, eca41, is mu-like and is able to integrate into, and excise from, various genomic locations. the other, eca29, is a p2 family prophage, and is also able to excise from the genome. excision of both prophages is rare and we were unable to induce lysis of cultures. deletion of the entire prophages, both separately and in combination, did not affect the growth rate or the secretion of plant cell wal ... | 2010 | 20146746 |
| quorum sensing-controlled evr regulates a conserved cryptic pigment biosynthetic cluster and a novel phenomycin-like locus in the plant pathogen, pectobacterium carotovorum. | pectobacterium carotovorum scri193 is a phytopathogenic gram-negative bacterium. in this study, we have identified a novel cryptic pigment biosynthetic locus in p. carotovorum scri193 which we have called the pectobacterium orange pigment (pop) cluster. the pop cluster is flanked by two trna genes and contains genes that encode non-ribosomal peptide synthases and polyketide synthase and produces a negatively charged polar orange pigment. orange pigment production is activated when an adjacent tr ... | 2010 | 20192973 |
| aepa of pectobacterium is not involved in the regulation of extracellular plant cell wall degrading enzymes production. | plant cell wall degrading enzymes (pcwde) are the major virulence determinants in phytopathogenic pectobacterium, and their production is controlled by many regulatory factors. in this study, we focus on the role of the aepa protein, which was previously described to be a global regulator of pcwde production in pectobacterium carotovorum (murata et al. in mol plant microbe interact 4:239-246, 1991). our results show that neither inactivation nor overexpression of aepa affects pcwde production in ... | 2010 | 20386924 |
| regulation of type vi secretion gene clusters by sigma54 and cognate enhancer binding proteins. | type vi secretion systems (t6ss) are bacteriophage-derived macromolecular machines responsible for the release of at least two proteins in the milieu, which are thought to form an extracellular appendage. although several t6ss have been shown to be involved in the virulence of animal and plant pathogens, clusters encoding these machines are found in the genomes of most species of gram-negative bacteria, including soil, marine, and environmental isolates. t6ss have been associated with several ph ... | 2011 | 21378190 |
| lpxc and yafs are the most suitable internal controls to normalize real time rt-qpcr expression in the phytopathogenic bacteria dickeya dadantii. | quantitative rt-pcr is the method of choice for studying, with both sensitivity and accuracy, the expression of genes. a reliable normalization of the data, using several reference genes, is critical for an accurate quantification of gene expression. here, we propose a set of reference genes, of the phytopathogenic bacteria dickeya dadantii and pectobacterium atrosepticum, which are stable in a wide range of growth conditions. | 2011 | 21637857 |
| quantitative resistance of potato to pectobacterium atrosepticum and phytophthora infestans: integrating pamp-triggered response and pathogen growth. | while the mechanisms underlying quantitative resistance of plants to pathogens are still not fully elucidated, the pathogen-associated molecular patterns (pamps)-triggered response model suggests that such resistance depends on a dynamic interplay between the plant and the pathogen. in this model, the pathogens themselves or elicitors they produce would induce general defense pathways, which in turn limit pathogen growth and host colonisation. it therefore suggests that quantitative resistance i ... | 2011 | 21853112 |
| control of potato soft rot caused by pectobacterium carotovorum and pectobacterium atrosepticum by moroccan actinobacteria isolates. | pectobacterium carotovorum and pectobacterium atrosepticum are dreadful causal agents of potato soft rot. actually, there are no efficient bactericides used to protect potato against pectobacterium spp. biological control using actinobacteria could be an interesting approach to manage this disease. thus, two hundred actinobacteria isolated from moroccan habitats were tested for their ability to inhibit in vitro 4 environmental pectobacterium strains and the two reference strains (p. carotovorum ... | 2011 | 22806806 |
| microbial fermentation of cabbage by a bacterial strain of pectobacterium atrosepticum for the production of bioactive material against candida species. | the objective of this study was to produce the bioactive fermented product by the microbial fermentation of cabbage (brassica oleracea) using a bacterial strain pectobacterium atrosepticum which was assessed for its antimycotic efficacy against pathogenic isolates of candida species. | 2011 | 23177810 |
| a multi-repeat adhesin of the phytopathogen, pectobacterium atrosepticum, is secreted by a type i pathway and is subject to complex regulation involving a non-canonical diguanylate cyclase. | cyclic diguanylate (c-di-gmp) is a second messenger controlling many important bacterial processes. the phytopathogen pectobacterium atrosepticum scri1043 (pba1043) possesses a type i secretion system (t1ss) essential for the secretion of a proteinaceous multi-repeat adhesin (mrp) required for binding to the host plant. the genes encoding the mrp and the t1ss are tightly linked to genes encoding several putative c-di-gmp regulatory components. we show that c-di-gmp regulates secreted mrp levels ... | 2011 | 21992096 |
| Chemical structure of the O-polysaccharide isolated from Pectobacterium atrosepticum SCRI 1039. | The lipopolysaccharide (LPS) of the bacterium Pectobacterium atrosepticum SCRI 1039 was hydrolyzed and the products were separated. A study of the obtained O-polysaccharide by means of chemical methods, GLC, GLC-MS, and NMR spectroscopy allowed us to identify a branched polymer with a pentasaccharide repeating unit of the structure shown below, in which the fucose residue was partially O-acetylated at C-2, C-3 or C-4. | 2011 | 22074675 |
| n-acetylglucosamine-dependent biofilm formation in pectobacterium atrosepticum is cryptic and activated by elevated c-di-gmp levels. | the phytopathogenic bacterium pectobacterium atrosepticum (pba) strain scri1043 does not exhibit appreciable biofilm formation under standard laboratory conditions. here we show that a biofilm-forming phenotype in this strain could be activated from a cryptic state by increasing intracellular levels of c-di-gmp, through overexpression of a constitutively active diguanylate cyclase (pled*) from caulobacter crescentus. randomly obtained pba transposon mutants defective in the pga operon, involved ... | 2011 | 21948048 |