Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| identification, characterization, and expression of a novel p450 gene encoding cyp6ae25 from the asian corn borer, ostrinia furnacalis. | an allele of the cytochrome p450 gene, cyp6ae14, named cyp6ae25 (genbank accession no. eu807990) was isolated from the asian com borer, ostrinia fumacalis (guenée) (lepidoptera: pyralidae) by rt-pcr. the cdna sequence of cyp6ae25 is 2315 bp in length and contains a 1569 nucleotides open reading frame encoding a putative protein with 523 amino acid residues and a predicted molecular weight of 59.95 kda and a theoretical pi of 8.31. the putative protein contains the classic heme-binding sequence m ... | 0 | 21529257 |
| hexokinase is a key regulator of energy metabolism and ros activity in insect lifespan extension. | developmental arrest (diapause) is a 'non-aging' state that is similar to the caenorhabditis elegans dauer stage and drosophila lifespan extension. diapause results in low metabolic activity and a profound extension of insect lifespan. here, we cloned the helicoverpa armigera hexokinase (hk) gene, a gene that is critical for the developmental arrest of this species. hk expression and activity levels were significantly increased in nondiapause-destined pupae compared with those of diapause-destin ... | 0 | 26852422 |
| development associated profiling of chitinase and microrna of helicoverpa armigera identified chitinase repressive microrna. | expression of chitinase is developmentally regulated in insects in consonance with their molting process. during the larval-larval metamorphosis in helicoverpa armigera, chitinase gene expression varies from high to negligible. in the five-day metamorphic course of fifth-instar larvae, chitinase transcript is least abundant on third day and maximal on fifth day. microrna library prepared from these highest and lowest chitinase-expressing larval stages resulted in isolation of several mirnas. in ... | 0 | 23887392 |
| bioassay of a nuclear-polyhedrosis virus and bacillus thuringiensis against the american bollworm, heliothis armigera, in botswana. | 1974 | 4597764 | |
| weight-related susceptibility of larvae of heliothis armigera to a crude nuclear-polyhedrosis virus preparation. | 1974 | 4597767 | |
| isolation of a cytoplasmic polyhedrosis virus by physical and immunological techniques. | procedures are described for the separation and purification of cytoplasmic polyhedrosis viruses obtained from multiply infected heliothis armigera larvae. separation was achieved by differential centrifugation and density gradient zone electrophoresis followed by complexing with nuclear polyhedrosis virus specific antibody. the yield of cytoplasmic polyhedrosis virus was increased by passage in larvae reared on synthetic media. | 1975 | 1098037 |
| the multicomponent genome of a different cytoplasmic polyhedrosis virus isolated from heliothis armigera. | the genome of a new cytoplasmic polyhedrosis virus (cpv) isolated from heliothis armigera has been shown to consist of 10 double-stranded rna segments, as in other cpvs, but the genome profile on page bears no resemblance to previously described cpvs isolated from h.armigera. | 1978 | 361635 |
| some physical characteristics of a cytoplasmic polyhedrosis virus of heliothis armigera. | a number of physical properties of a cytoplasmic polyhedrosis virus of heliothis armigera were determined. these included a buoyant density of 1.44 g/cm3, a diffusion coefficient of 0.73 x 10(-7) cm2/sec, a partial specific volume of 0.688 ml/g, and a sedimentation coefficient of 399s. three different methods were used to determine an average molecular weight of 47.4 x 10(6). | 1980 | 6987192 |
| radioimmunoassay analysis of baculovirus granulins and polyhedrins. | granulin and polyhedrin proteins were purified by preparative sodium dodecyl sulfate (sds)-polyacrylamide gel electrophoresis from the baculoviruses autographa californica, rachiplusia ou, heliothis zea, heliothis armigera, trichoplusia ni, and spodoptera frugiperda. antisera were raised against autographa californica (ac) polyhedrin and trichoplusia ni (tn) granulin and analyzed for homologous and heterologous immunoreactivity by immunodiffusion and radioimmunoassay (ria). ac polyhedrin and tn ... | 1980 | 18631650 |
| induction of a nonoccluded baculovirus persistently infecting heliothis zea cells by heliothis armigera and trichoplusia ni nuclear polyhedrosis viruses. | a nonoccluded singly enveloped baculovirus (baculovirus x) persistently infects heliothis zea (imc-hz-1) cells in culture. singly enveloped nuclear polyhedrosis viruses from h. zea and heliothis armigera, and multiply enveloped nuclear polyhedrosis viruses from trichoplusia ni, spodoptera frugiperda, and spodoptera littoralis were all found to induce baculovirus x. experiments are reported which use metabolic inhibitors and inactivated inducing virus to show that it is probable that a structural ... | 1981 | 18635066 |
| virus envelope acquisition of nuclear polyhedrosis virus in vitro. | there have been several reports concerned with the replication and morphogenesis of insect baculoviruses during the past decade [1--7]. while there is general agreement as to the assembly of the virus on the basis of electron microscopic studies, there are still questions regarding the details of the replicative mechanisms, such as the acquisition of the virus envelope. three possible ways have been proposed to describe envelope formation of nuclear polyhedrosis virus: (i) acquisition of budding ... | 1981 | 7018096 |
| midgut and viral associated proteases of heliothis armigera. | the role played by the gut juice of insects in the infective process of insect viruses was examined. analysis of larval gut extract of heliothis armigera by sds-page revealed protease activity associated with components of molecular weights 48,000 and 94,000. proteases were found to be associated with occlusion bodies and virions of both nuclear polyhedrosis virus (npv) and cytoplasmic polyhedrosis virus (cpv) infecting h. armigera. cpv occlusion bodies were dissolved by gut juice extract at ph ... | 1983 | 6337972 |
| utilization of fodder yeast and agro-industrial by-products in production of spores and biologically - active endotoxins from bacillus thuringiensis. | a number of newly-devised fermentation media were evaluated with respect to their ability to support sporulation and biosynthesis of endotoxins by strains of bacillus thuringiensis that are biologically active against spodoptera littoralis, heliothis armigera, and spodoptera exigua. fodder yeast from dried cells of saccharomyces cerevisiae could be used as a complete mono-component medium for production of highly active spore-delta-endotoxin complexes from b. thur., vars. entomocidus, kurstaki a ... | 1983 | 6666415 |
| comparison of sequence diversity in several cytoplasmic polyhedrosis viruses. | sequence homology among several different cytoplasmic polyhedrosis virus (cpv) types and the human reovirus (type 1) was examined by northern blot analysis and s, nuclease analysis, using random-primed cdna probes synthesized from total genomic rna. the results show no homology among the cpv type 1, bombyx mori cpv, type 5, orgyia pseudotsugata cpv, type 8, manduca sexta cpv and the human reovirus (type 1). however, there was significant homology among three type 5 cpvs, o. pseudotsugata cpv, eu ... | 1983 | 18639153 |
| dna homology relationships between spodoptera littoralis nuclear polyhedrosis virus and other baculoviruses. | the dna sequence homology relationships between spodoptera littoralis nuclear polyhedrosis virus (npv) and five other lepidopteran npvs were studied by hybridization of 32p-labeled npv dnas to southern blots of restriction-endonuclease-digested npv dna. the s. littoralis npv (slmnpv) shows extensive dna sequence homology through most of the genome with s. frugiperda npv and s. exigua npv. heliothis armigera npv, h. zea npv, and autographa californica npv (acmnpv) share fewer regions of homology ... | 1985 | 2995274 |
| [properties of the nuclear polyhedrosis virus]. | four passages of barathra brassicae l nuclear polyhedrosis virus (npv) in heliothis armigera hbn caterpillars were carried out. b. brassicae npv was found to be highly infective for caterpillars of both species. when h. armigera caterpillars were infected with b. brassicae npv, reproduction of the latter occurred alongside with the induction of h. armigera latent virus. in subsequent passages the latent virus replaced the foreign npv. it was noted in mixed infection that in the 2nd passage the a ... | 1985 | 3913136 |
| cricket paralysis virus, a potential control agent for the olive fruit fly, dacus oleae gmel. | representatives of several families of insect viruses were tested for growth and pathogenicity in the olive fruit fly, dacus oleae gmel. the viruses included nuclear polyhedrosis viruses, an iridovirus, two picornaviruses, and trichoplusia ni small rna virus (a member of the nudaurelia beta family), in addition to two naturally occurring viruses of the olive fruit fly. two viruses, one of the two picornaviruses (cricket paralysis virus [crpv] and the iridovirus (type 21 from heliothis armigera), ... | 1987 | 16347255 |
| cytoplasmic polyhedrosis virus classification by electropherotype; validation by serological analyses and agarose gel electrophoresis. | serological analyses of several different cytoplasmic polyhedrosis viruses (cpvs), including two type 1 cpvs from bombyx mori, type 1 cpv from dendrolimus spectabilis, type 12 cpv from autographa gamma, type 2 cpv from inachis io, type 5 cpv from orgyia pseudotsugata and type 5 cpv from heliothis armigera, demonstrated a close correlation between the antigenic properties of the polyhedrin or virus particle structural proteins and the genomic dsrna electropherotypes. the dsrnas of these viruses w ... | 1989 | 2499658 |
| role of insects as inhalant allergens in bronchial asthma with special reference to the clinical characteristics of patients. | the whole body extracts (wbes) of 13 common insects from delhi, namely musca domestica (house fly); sitophilus oryzae (rice weevil); callosobrochous maculatus (pulse beetle); anopheles stephensi, culex quinquefasciatus and aedes aegypti (mosquitoes); blattella germanica and periplaneta americana (cockroaches: male, female and nymph); spodoptera litura and heliothis armigera (moths), were prepared to evaluate their allergenic significance in patients with allergic bronchial asthma. intradermal (i ... | 1990 | 2253082 |
| bacillus thuringiensis potency bioassays against heliothis armigera, earias insulana, and spodoptera littoralis larvae based on standardized diets. | bacillus thuringiensis screening programs based on the official potency bioassay using third-instar larvae and on a neonate bioassay were developed for heliothis armigera, earias insulana, and spondoptera littoralis. in these bioassays, the diets were standardized to be suitable, with minor modifications, for feeding of the three lepidopterans. the bioassay protocol was based on determination of the lc50 of the microbial standard hd-1-s-80 in the insects susceptible to b. thuringiensis var. kurs ... | 1990 | 2351843 |
| location and nucleotide sequence of the gene encoding the viral enhancing factor of the trichoplusia ni granulosis virus. | the gene encoding the viral enhancing factor (vef) of trichoplusia ni granulosis virus has been cloned from a lambda gt11 expression library, and the complete nucleotide sequence determined. the vef gene encodes a protein with a predicted mr of 104k which does not share homology with any previously reported proteins. a possible promoter is located four nucleotides upstream of the initiation codon and represents a consensus baculovirus late promoter (ataag). this has been confirmed by the identif ... | 1991 | 1940861 |
| photoprotection of bacillus thuringiensis kurstaki from ultraviolet irradiation. | irradiation of bacillus thuringiensis var. kurstaki hd1 at 300-350 nm for up to 12 hr using a photochemical reactor results in a rapid loss of its toxicity to larvae of heliothis armigera. photoprotection of the toxic component was obtained by adsorption of cationic chromophores such as acriflavin (af), methyl green, and rhodamine b to b. thuringiensis. af gave the best photoprotection and a level of 0.42 mmol/g dye absorbed per gram of b. thuringiensis was highly toxic even after 12 hr of ultra ... | 1991 | 2066575 |
| the insecticidal crystal protein cryia(c) from bacillus thuringiensis is highly toxic for heliothis armigera. | 1992 | 1541846 | |
| a gene encoding a highly expressed spindle body protein of heliothis armigera entomopoxvirus. | the gene encoding the most abundant protein of purified preparations of heliothis armigera entomopoxvirus (haepv) has been cloned and sequenced. the gene sequence encodes a 40.1k polypeptide with a putative n-terminal 20 amino acid leader peptide, and a single potential n-glycosylation site. analysis of the protein, which has an apparent m(r) of 50k on polyacrylamide gels, confirmed post-translational loss of the leader peptide, but showed no evidence of glycosylation. the protein is related to ... | 1993 | 8376960 |
| toxicity, penetration, tissue distribution, and metabolism of methyl parathion in helicoverpa armigera and h. punctigera (lepidoptera: noctuidae). | toxicity of c14-ring-methyl parathion in penetration, tissue absorption, and metabolism by susceptible helicoverpa armigera (hübner) and helicoverpa punctigera (wallengren) larvae was compared. c14 methyl parathion penetrated the cuticle of h. punctigera more rapidly than of h. armigera, leading to initially higher internal insecticide concentrations in h. punctigera. the same methyl-parathion metabolites were recovered from h. armigera and h. punctigera. however, larger quantities of methyl par ... | 1994 | 7962948 |
| analysis of the c-polyhedrin genes from different geographical isolates of a type 5 cytoplasmic polyhedrosis virus. | the c-polyhedrin genes of two different geographic isolates of a type 5 cytoplasmic polyhedrosis virus (cpv) were cloned. a cpv infecting orgyia pseudotsugata (opcpv), isolated in the pacific northwest of the u.s.a., and a cpv infecting heliothis armigera, isolated in south africa, were studied. both genes were found to be 883 nucleotides in length and encoded a predicted protein of 246 residues (m(r) of 28,890). comparison of the nucleotide sequences of these two viruses with another type 5 geo ... | 1994 | 8046399 |
| expression of beta-galactosidase and luciferase in insect cell lines infected with a recombinant acmnpv. | an acmnpv recombinant (ac-gal-luc) carrying the beta-galactosidase and luciferase genes under the control of the p10 and polyhedrin promoters, respectively, was used to study expression in nine insect cell lines. all acmnpv-permissive cell lines expressed both reporter genes with the coleopteran cell line, anthonomus grandis (age), producing the highest concentrations of beta-galactosidase (5.0 x 10(6) pg/ml) and luciferase (2.67 x 10(3) pg/ml). both enzymes were detected as early as 12 h postin ... | 1994 | 8069450 |
| introduction of a lepidopteran-specific insecticidal crystal protein gene of bacillus thuringiensis subsp. kurstaki by conjugal transfer into a bacillus megaterium strain that persists in the cotton phyllosphere. | a lepidopteran toxin gene of the entomopathogen bacillus thuringiensis subsp. kurstaki hd-1 was introduced into a cotton leaf-colonizing bacillus megaterium strain, rs1, by conjugal transfer. rifampin- and nalidixic acid-resistant colonies obtained after cell mating were screened for crystal production by microscopy. a transcipient, b. megaterium rs1-43, was selected by this procedure. southern blot hybridization with both total dna and hindiii-digested dna of the transcipient showed positive si ... | 1994 | 16349152 |
| sequence of rna2 of the helicoverpa armigera stunt virus (tetraviridae) and bacterial expression of its genes. | the complete nucleotide sequence of rna2 of helicoverpa armigera stunt virus (hasv), a member of the tetraviridae, was determined by characterization of cloned cdna and pcr products and direct sequencing of genomic rna. the capped, positive sense, single-stranded rna is 2478 nucleotides in length and has two overlapping open reading frames (orfs) likely to be cistrons which are situated between terminal non-coding regions of 282 and 168 bases, 5' and 3', respectively. extensive secondary structu ... | 1995 | 9049325 |
| [characters and insecticidal polypeptide of a new strain of bacillus thuringiensis subsp. kenyae in china]. | a new strain of bacillus thurinigiensis bt with high toxicity against noctuidae larvae has been screened for many generations since isolated from larvae candle of aphomia gularis in yiyang county, jiangxi province, in china. by comparison and analysis of results of physiological and biochemical test, flagella antigen identification and esterase type, the strain is identified as h4a-4c bt serovar. kenyae. since its crystal protein type and plasmid type are different from those of bt serovar. keny ... | 1995 | 7483588 |
| functional expression of an alpha anti-insect scorpion neurotoxin in insect cells and lepidopterous larvae. | the leiurus quinquestriatus hebraeus alpha anti-insect toxin (lqh alpha it) cdna was engineered into the autographa californica nuclear polyhedrosis virus (acnpv) genome. insect cells infected with the recombinant virus secreted a functional lqh alpha it polypeptide. spodoptera littoralis and heliothis armigera larvae injected with recombinant budded virus, showed typical intoxication symptoms. this recombinant virus showed enhanced insecticidal potency against h. armigera larvae compared with w ... | 1995 | 7498537 |
| highly insect-resistant transgenic tobacco plants containing both b.t. and cpti genes. | the cowpea trypsin inhibitor (cpti) gene was synthesized according to its cdna sequence using dna synthesizer and confirmed by dna sequencing. the cpti gene and modified bacillus thuringiensis (b.t.) delta-endotoxin gene were cotransformed to tobacco explants mediated by agrobacterium tumefaciens. the integrations of b.t and cpti genes were confirmed by pcr and southern hybridization. three kinds of transgenic plants were obtained: (1) containing cpti gene, (2) containing b.t gene, (3) containin ... | 1995 | 7548766 |
| the larger genomic rna of helicoverpa armigera stunt tetravirus encodes the viral rna polymerase and has a novel 3'-terminal trna-like structure. | in this paper we report the complete nucleotide sequence of the larger segment (5312 nucleotides) of the bipartite rna genome of helicoverpa armigera stunt tetravirus (hasv). hasv therefore becomes the first member of the tetraviridae, a virus family with a host range restricted to lepidopteran insects, whose genome has been completely sequenced. hasv rna 1 encodes a 187k protein which includes three domains conserved in rna-dependent rna polymerases of rna viruses in the alpha-like superfamily. ... | 1995 | 11831734 |
| [distribution of bacillus thuringiensis in soils of north and south of china]. | 221 isolates of bacillus thuringiensis were isolated in 1491 soil samples from north and south of china. h-serotypes and larvicidal characters of all bt isolates have been identified. the rate of bt-harbouring soil sample and the rate of bt isolates in northeast and neimeng were in 12.6% and 17.2% respectively. predominant serotypes were h4, h10, h3, h13, h5 and h29. the most fertile bt-harbouring area was the heilongjiang province with rate of bt-harbouring sample of 21.4% and rate of bt isolat ... | 1996 | 9639831 |
| an entomopoxvirus homologue of the vaccinia virus d13l-encoded 'rifampicin resistance' protein. | the heliothis armigera entomopoxvirus (haepv) genome encodes a predicted 68 kda polypeptide related to the 'rifampicin resistance' protein of vaccinia virus (with 30 % identity), and an homologous swinepox virus protein (27% identity). we were unable to isolate an haepv genotypic variant encoding a predicted c-terminal truncated form of the protein, suggesting that the c terminus of the molecule may be essential to protein function, and, in turn, that this function may be essential to viral repl ... | 1996 | 8609479 |
| [construction of shuttle vector containing delta-endotoxin gene of bacillus thuringiensis]. | the bacillus thuringiensis toxin gene cryia(c) was inserted into the shuttle vector pbe-2 to construct pamy for expressing the b.t. gene in both gram-negative and -positive bacterial systems. pamy was introduced into wild type bacillus cereus, b.brevis and b.subtilis by electroporation. transformants containing delta-endotoxin gene produced proteins reacted with b.t. crystal protein antibody. upon biological toxicity tests, the transformants gave a mortality of 100% against ostrinia furnacilis, ... | 1996 | 8762474 |
| cloning and sequencing of the gene for an enhancing factor from pseudaletia separata entomopoxvirus. | an enhancing factor (ef) has been shown to increase the susceptibility of the armyworm, pseudaletia separata, to this nuclear polyhedrosis virus. using amino acid microsequencing data obtained from the purified ef protein, we cloned and sequenced the gene for ef. the deduced amino acid sequence exhibited a significant similarity to major spindle proteins, fusolins, whose function is unknown, from heliothis armigera, choristoneura biennis and melolontha melolontha entomopoxviruses. | 1996 | 8921880 |
| are odorant-binding proteins involved in odorant discrimination? | pheromone-sensitive sensilla trichodea of nine moth species belonging to six families and three superfamilies of lepidoptera were immunolabelled with an antiserum against the pheromone-binding protein of antheraea polyphemus. strong immunolabelling of the sensillum lymph was observed in all long sensilla trichodea of a. polyphemus, a. pernyi (saturniidae), bombyx mori (bombycidae) and manduca sexta (sphingidae). very weak labelling was found with all sensilla trichodea of dendrolimus kikuchii (l ... | 1996 | 8985600 |
| behavioral response of femalehelicoverpa (heliothis)armigera hb. (lepidoptera: noctuidae) moths to synthetic pigeonpea (cajanus cajan l.) kairomone. | femaleh. armigera moths are highly attracted by a steam distillate from pigeonpea plants, one of their main hosts. a mixture of six compounds, all sesquiterpenes (β-caryophyllene,α-humulene,α-guajene,α-muurolene,γ-muurolene, andα-bulnesene), mixed in the proportions as found in the steam distillate, elicited the same behavioral responses (oriented upwind flights and contacts with the odor source) as the steam distillate. onlyα-bulnesene was attractive by itself, but still less than the whole mix ... | 1996 | 24227588 |
| mobility of hat transposable elements in the old world bollworm, helicoverpa armigera. | the p, hobo, hermes, minos and mariner transposable elements have been successfully used as gene vectors to achieve genetic transformation of drosophila melanogaster. the hobo transposable element of d. melanogaster, a member of the hat family of transposable elements, can also transpose in other dipteran species including the house fly musca domestica and the queensland fruit fly, bactrocera tryoni. another hat element, hermes, can also transpose in a wide range of insect species. we report her ... | 1996 | 8933173 |
| effects of infected insects on secondary invasion of steinernematid entomopathogenic nematodes. | factors affecting 'invasion efficiency' of steinernematid entomopathogenic nematodes into hosts were elucidated. the phenomenon that only part (10-40%) of the nematode population invades the target host has been recorded in many studies. it has been mainly ascribed to differences in the ability of individual nematodes to infect. in the present study the effect of an infected host, the wax moth galleria mellonella, on subsequent infection of the entomopathogenic nematodes steinernema carpocapsae ... | 1997 | 9172429 |
| evidence for gene conversion between tandemly duplicated cytoplasmic actin genes of helicoverpa armigera (lepidoptera:noctuidae) | tandemly duplicated actin genes have been isolated from a helicoverpa armigera genomic library. sequence comparisons with actin genes from other species suggest they encode cytoplasmic actins, being most closely related to the bombyx mori a3 actin gene. the duplicated h. armigera actin genes, termed a3a and a3b, share 98.3% nucleotide sequence identity over their entire putative coding region. analysis of the distribution of nucleotide differences shows the first 763 bp are identical between the ... | 1997 | 9069177 |
| complete nucleotide sequence of the fusolin gene of an entomopoxvirus in the cupreous chafer, anomala cuprea hope (coleoptera: scarabaeidae). | the fusolin gene of anomala cuprea entomopoxvirus (acepv) was cloned and sequenced. the sequence was compared with that of fusolin genes of epvs from other insects such as melolontha melolontha (mmepv), choristoneura biennis (cbepv) and heliothis armigera (haepv) previously reported. the gene harbored a single open reading frame (orf) of 1119 nt capable of coding for a protein of 43.3 kda. the microsequencing of the protein showed the cleavage of a signal peptide of 16 amino acid (aa) residues. ... | 1997 | 9474783 |
| laboratory screening of different bacillus thuringiensis strains against certain lepidopteran pests and subsequent field evaluation on the pod boring pest complex of pigeonpea (cajanus cajan). | laboratory evaluation of different bacillus thuringiensis subspecies revealed that b. thuringiensis subsp. kurstaki (ncim 2514) at 10(8) spores/ml concentration caused more than 85% mortality to the neonate larvae of the lepidopteran insects spodoptera litura (f.) and phthorimaea operculella (z.). this strain at 10(10) spores/ml concentration was effective against the major lepidopteran pests comprising the pod boring complex of pigeonpea (cajanus cajan), viz. helicoverpa armigera (h.) and exela ... | 1997 | 9195006 |
| cytochrome p450 genes from helicoverpa armigera: expression in a pyrethroid-susceptible and -resistant strain. | the molecular basis of metabolic resistance to pyrethroids in helicoverpa armigera is currently under debate. substantial indirect evidence supports a role for both esterase- and cytochrome-p450-mediated metabolism. however, the relative roles played by these two mechanisms in field-based resistance is uncertain. our understanding of the importance of p450-mediated metabolism is hindered by the paucity of cloned genes from this species, and the corresponding absence of data on rates of insectici ... | 1997 | 9304792 |
| cytochrome p450 (cyp)9a1 in heliothis virescens: the first member of a new cyp family. | a novel cytochrome p450 cdna with its complete coding sequence and part or all of the 3' (77 nucleotides) and 5' (87 nucleotides) non-coding sequence was isolated from the tobacco budworm, heliothis virescens (f). the 1763 nucleotide sequence encodes a protein of 532 amino acids which includes a hydrophobic n-terminal region and the highly conserved heme binding regions typical of p450s. low sequence similarity to other p450 sequences and the presence of a thromboxane synthase-like insertion ups ... | 1997 | 9304798 |
| molecular analysis of an enhancin gene in the lymantria dispar nuclear polyhedrosis virus. | a lymantria dispar nuclear polyhedrosis virus (ldmnpv) gene has been identified that encodes a homolog to the granulovirus (gv) enhancin proteins that are capable of enhancing the infection of other baculoviruses. enhancin genes have been identified and sequenced for three species of gvs but have not been found in any other nuclear polyhedrosis virus to date. the ldmnpv enhancin gene is located between 67.6 and 70.1 kbp on the viral genome. northern and primer extension analyses of viral rnas in ... | 1997 | 9343163 |
| lepidopteran peptides of the allatostatin superfamily. | peptides of the allatostatin superfamily with the c-terminal amino acid sequence -yxfgl-nh2 have been isolated and identified from the lepidopterans, the codling moth, cydia pomonella (tortricidae) and the bollworm, helicoverpa armigera (noctuidae). the peptides, designated cydiastatins and helicostatins respectively, were monitored during purification with radioimmunoassays based on the callatostatins of the blowfly calliphora vomitoria. the eight peptides from each of the two species appear to ... | 1997 | 9392829 |
| analysis of the ecdysteroid udp-glucosyltransferase gene of heliothis armigera single-nucleocapsid baculovirus. | an ecdysteroid udp-glucosyltransferase (egt) gene was identified from the single (s) nucleocapsid nucleopolyhedrovirus of heliothis armigera (hearnpv). in baculovirus-infected insects the viral enzyme (egt) plays a pivotal role in abrogating the insect molting process. the open reading frame of the egt gene is 1545 nucleotides long, encoding a putative protein of 515 amino acids with a mr of 59.1. the 5'-noncoding region contains a putative early (cagt) and late (taag) motif for transcription in ... | 1997 | 9482587 |
| nucleotide sequence analysis of hasnpv protein kinase. | the protein kinase gene of helicoverpa armigera single nucleocapsid nuclear polyhedrosis virus (hasnpv) has been cloned and sequenced. it is located approximately 1.25 kb downstream of the polyhedrin gene. the predicted molecular mass of this 267 amino acid protein (havpk) is 31 kd. havpk shows a 43.0% amino acid homology to vpk from lymantria dispar multinucleocapsid nuclear polyhedrosis virus (ldmnpv) and a 39.0% homology to pk-1 from autographa californica multinucleocapsid nuclear polyhedros ... | 1997 | 12219213 |
| novel mutations in the para-homologous sodium channel gene associated with phenotypic expression of nerve insensitivity resistance to pyrethroids in heliothine lepidoptera. | coding sequences from the intracellular segment linking repeat domains iii and iv of the para-homologous sodium channel gene were amplified and cloned from two species of heliothine larvae using rt-pcr techniques. sequence comparisons between standard laboratory susceptible and nerve insensitive strains of the tobacco budworm (heliothis virescens) and the cotton bollworm (helicoverpa armigera) identified two mutations. these were an aspartic acid (gac) to valine (gtc) and a glutamic acid (gaa) t ... | 1998 | 9535164 |
| in vitro and in vivo host range of anticarsia gemmatalis multiple nuclear polyhedrosis virus. | a clone of the wild type (wt) anticarsia gemmatalis multiple nuclear polyhedrosis virus agmnpv, derived from a geographical isolate (hondrina, brazil) and designated agmnpv-cl4-3a1, was used to determine the host range of this virus in six established lepidopteran cell lines: anticarsia gemmatalis (bcirl-ag-am1), helicoverpa zea (bcirl-hz-am1), heliothis virescens (bcirl-hv-am1), helicoverpa armigera (bcirl-ha-am1), trichoplusia ni (tn-cl1), bombyx mori (bmn), and a coleopteran cell line anthono ... | 1998 | 9542640 |
| processing of delta-endotoxin of bacillus thuringiensis subsp. kurstaki hd-1 in heliothis armigera midgut juice and the effects of protease inhibitors. | bombyx mori was found to be more sensitive to the protoxins of hd-1 than heliothis armigera. sds-page analysis showed that a large amount of activated toxin was yielded from protoxin by b. mori gut juice while little was yielded by h. armigera. further degradation of activated toxin was observed in h. armigera midgut juice detected by sds-page. ph influenced the proteolytic activity of the midgut juice significantly, but there was no obvious effect of ph on the degradation of activated toxin. sp ... | 1998 | 9647704 |
| a gloverin-like antibacterial protein is synthesized in helicoverpa armigera following bacterial challenge. | a bacteria inducible antibacterial protein, p2, was isolated from the old world bollworm helicoverpa armigera. fifth-instar larvae were injected with live escherichia coli nctc 8196. p2 was isolated by hplc using reversed-phase and size-exclusion columns. in addition, p2 was isolated by an alternative method of sequential cation-exchange and reversed-phase hplc. the structure of p2 was determined by n-terminal edman degradation and mass spectrometry. p2 had similar mass (14.1 kda) structure and ... | 1998 | 9699484 |
| environmental distribution and diversity of bacillus thuringiensis in spain. | bacillus thuringiensis was isolated from 301 out of 1,005 samples collected in spain from agricultural and non-cultivated soils, dust from stored products, and dead insects. based on the production of parasporal crystals, 1,401 isolates were identified as b. thuringiensis after examining 11,982 b. thuringiensis-like colonies. we found a greater presence of b. thuringiensis in dust from grain storages than in other habitats. sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the spore-c ... | 1998 | 9741114 |
| efficacy of lepidopteran specific delta-endotoxins of bacillus thuringiensis against helicoverpa armigera | 1998 | 9784360 | |
| amylase inhibitors of pigeonpea (cajanus cajan) seeds. | pigeonpea (cajanus cajan l) seeds were analysed quantitatively for amylase inhibitor (ai) activity and qualitatively, by an in-gel-detection method on polyacrylamide gels. at least four ai isoforms were identified in pigeonpea seeds. the ais inhibit human salivary and bovine pancreatic amylase but fail to inhibit bacterial, fungal and endogenous amylase. pigeonpea ais were found to be active over a ph range of 4.5 to 9.5 and were heat labile. the isoelectric point of a major inhibitor is 6.2 ais ... | 1998 | 9431672 |
| learning in host selection in helicoverpa armigera (hubner) (lepidoptera: noctuidae) | the effect of experience on pre- and post-alighting host selection in adult female helicoverpa armigera was tested in an indoor flight cage, and in a large greenhouse. the moths had experienced either tobacco or tomato plants (both are hosts of h. armigera) for 3 days, or were given no experience. individuals were then released and their host selection assessed. all individuals caught in the greenhouse were identified and tested for post-alighting acceptance on each host. experience significantl ... | 1998 | 9480690 |
| serial passage of a helicoverpa armigera nucleopolyhedrovirus in helicoverpa zea cell cultures. | the serial passaging of baculoviruses in cell lines numerous times can result in a variety of mutations or defective viral populations becoming predominant in the cultures. the generation of these mutants during cell culture passage, also known as "the passage effect," can seriously hinder the use of in vitro methods for large-scale production of baculoviruses for use as biopesticides. in an effort to develop a large-scale in vitro method of producing helicoverpa armigera singly enveloped nucleo ... | 1999 | 10222185 |
| sequence of the genomic rna of nudaurelia beta virus (tetraviridae) defines a novel virus genome organization. | the monopartite genome of nudaurelia beta virus, the type species of the betatetravirus genus of the family tetraviridae, consists of a single-stranded positive-sense rna (ss+rna) of 6625 nucleotides containing two open reading frames (orfs). the 5' proximal orf of 5778 nucleotides encodes a protein of 215 kda containing three functional domains characteristic of rna-dependent rna polymerases of ss+rna viruses. the 3' proximal orf of 1836 nucleotides, which encodes the 66-kda capsid precursor pr ... | 1999 | 10329566 |
| the establishment of heliothine cell lines and their susceptibility to two baculoviruses. | a total of eight cell lines were established from helicoverpa armigera (3) and h. punctigera (5) embryos and ovaries. cell lines were established and grown in tc100 and/or tc199-mk containing 10% fetal bovine serum. the serum-free medlium excell 400 was also used, with and without 10% supplemental fetal bovine serum, but failed to generate cell lines from fat bodies, embryos, or ovarian tissues. cell lines consisted of heterogenous cell types ranging from oval to fibroblast-like. this is the fir ... | 1999 | 10475263 |
| amino acid substitution in alpha-helix 7 of cry1ac delta-endotoxin of bacillus thuringiensis leads to enhanced toxicity to helicoverpa armigera hubner. | insecticidal proteins or delta-endotoxins of bacillus thuringiensis are highly toxic to a wide range of agronomically important pests. the toxins are formed of three structural domains. the n-terminal domain is a bundle of eight alpha-helices and is implicated in pore formation in insect midgut epithelial membranes. all the delta-endotoxins share a common hydrophobic motif of eight amino acids in alpha-helix 7. a similar motif is also present in fragment b of diphtheria toxin (dt). site-directed ... | 1999 | 10481060 |
| the specificity of helicoverpa armigera stunt virus infectivity. | helicoverpa armigera stunt virus (hasv) is a member of the tetraviridae family of rna viruses whose replication and expression strategies are not well understood due to the absence of an in vitro cell culture system. we set out to find such a system for hasv by screening an array of 13 insect and 1 mammalian cell culture lines with both virus particle infection and genomic rna transfection. no cell line was found to be permissive for replication, although entry of genomic rna was verified. the a ... | 1999 | 10486228 |
| virions of heliothis armigera entomopoxvirus contain a homologue of the vaccinia vp8 major core protein. | an antigenic 30 k virion protein of heliothis armigera entomopoxvirus (haepv) has been identified as a homologue of the chordopoxvirus (chpv) vp8 major virion core protein. like its homologue in vaccinia virus, the mature haepv 30 k protein is derived by post-translational cleavage of a precursor at a conserved aga motif. the haepv 30 k protein is the first epv structural virion protein to be described, and elucidation of its characteristics provides evidence for the assumption that morphologica ... | 1999 | 10499447 |
| insecticidal activity of transgenic tobacco plants expressing both bt and cpti genes on cotton bollworm (helicoverpa armigera). | transgenic tobacco plants expressing both bacillus thuringiensis (bt) insecticidal protein and cowpea trypsin inhibitor (cpti) genes was used to evaluate the insecticidal activity on the cotton bollworm (helicoverpa armigera) compared with transgenic tobacco with bt insecticidal protein gene alone. mortality of first to third instar larvae fed on transgenic two genes tobacco for three days was significantly higher than that fed on transgenic bt tobacco. first to fourth instar larvae fed on trans ... | 1999 | 10668128 |
| drosophila melanogaster sex peptide stimulates juvenile hormone synthesis and depresses sex pheromone production in helicoverpa armigera. | previous studies demonstrate that virgin female adult helicoverpa armigera (lepidoptera: noctuidae) moths exhibit calling behaviour and produce sex pheromone in scotophase from the day after emergence, and that mating turns off both of these pre-mating activities. in the fruit fly drosophila melanogaster, a product of the male accessory glands, termed sex peptide (sp), has been identified as being responsible for suppressing female receptivity after transfer to the female genital tract during ma ... | 1999 | 12770380 |
| [the effects of reactive oxidants on bacillus thuringiensis parasporal crystals]. | the effects of hydrogen peroxides(h2o2) and hydroxyl radicals(.oh) on the purified parasporal crystals from bacillus thuringiensis var. kurstaki hd-1 were studied by sds-page and bioassay. the results showed that to some extent h2o2 and .oh produced a direct and deleterious effect. hydroxyl radicals from the system fe(ii)-edta-h2o2 indicated more serious damage than hydrogen peroxides. there was a 44.5% loss in activity against 12-h-old larvae of heliothis armigera when parasporal crystals were ... | 1999 | 12555530 |
| successive use of non-host plant proteinase inhibitors required for effective inhibition of helicoverpa armigera gut proteinases and larval growth | we report on the efficacy of proteinase inhibitors (pis) from three host plants (chickpea [cicer arietinum], pigeonpea [cajanus cajan], and cotton [gossypium arboreum]) and three non-host (groundnut [arachis hypogea], winged bean [psophocarpus tetragonolobus], and potato [solanum tuberosum]) in retarding the growth of helicoverpa armigera larvae, a devastating pest of important crop plants. enzyme assays and electrophoretic analysis of interaction of h. armigera gut proteinases (hgps) with pis r ... | 1999 | 10517841 |
| comparison of bombyx mori and helicoverpa armigera cytoplasmic actin genes provides clues to the evolution of actin genes in insects. | the cytoplasmic actin genes bma3 and bma4 of bombyx mori were found clustered in a single genomic clone in the same orientation. as a similar clustering of the two cytoplasmic actin genes ha3a and ha3b also occurs in another lepidopteran, helicoverpa armigera, we analyzed the sequence of the pair of genes from each species. due to the high conservation of cytoplasmic actins, the coding sequence of the four genes was easily aligned, allowing the detection of similarities in noncoding exon and int ... | 1999 | 10028284 |
| diversity in inhibitors of trypsin and helicoverpa armigera gut proteinases in chickpea (cicer arietinum) and its wild relatives. | developing seeds of eight chickpea (cicer arietinum l.) cultivars (12-60 days after flowering) showed a significant variation in the trypsin inhibitor (ti) and the helicoverpa armigera gut proteinase inhibitor (hgpi) content. for example, the highest ti (198 units/g) and hgpi (23 units/g) activities were exhibited by mature seeds of cv iccv-2, whereas the lowest inhibitor activities were observed in cv pg8505-7 (96.1 ti units/g) and cv vijay (5 hgpi units/g). electrophoretic patterns showed a va ... | 1999 | 22665210 |
| toxicity of cinnamomin--a new type ii ribosome-inactivating protein to bollworm and mosquito. | the toxicity of cinnamomin, a new type ii ribosome-inactivating protein purified from the seeds of camphor tree (cinnamomum camphora), to bollworm (helicoverpa armigera) and mosquito (culex pipines pallens) during larval stage was tested. the lc50 of cinnamomin to bollworm larvae fed on diet containing cinnamomin was 1839 ppm and the lc50 to larvae of mosquito was 168 ppm. the gut extract of bollworm larvae could apparently hydrolyze cinnamomin. the inhibition of protein synthesis by cinnamomin ... | 2000 | 10732994 |
| molecular cloning and genomic organization of an allatostatin preprohormone from drosophila melanogaster. | the insect allatostatins are neurohormones, acting on the corpora allata (where they block the release of juvenile hormone) and on the insect gut (where they block smooth muscle contraction). we screened the "drosophila genome project" database with electronic sequences corresponding to various insect allatostatins. this resulted in alignment with a dna sequence coding for some drosophila allatostatins (drostatins). using pcr with oligonucleotide primers directed against the presumed exons of th ... | 2000 | 10891383 |
| immunolocalization of general odorant-binding protein in antennal sensilla of moth caterpillars. | antennae of bombyx mori and helicoverpa armigera larvae were immunolabelled with antisera raised against the pheromone-binding protein or the general odorant-binding protein 2 of antheraea polyphemus to assign the expression of these proteins to individual sensilla and to compare the localization pattern with that in sensilla of adult moths. specific labelling of antennal sensilla was only obtained with the antiserum against general odorant-binding protein 2. among the few sensilla present on th ... | 2000 | 18088914 |
| identification of novel serine proteinase gene transcripts in the midguts of two tropical insect pests, scirpophaga incertulas (wk.) and helicoverpa armigera (hb.). | we have used rt pcr and 3'race to identify diverse serine proteinase genes expressed in the midguts of the rice yellow stem borer (scirpophaga incertulas) and asian corn borer (helicoverpa armigera). the rt-pcr primers encoded the conserved regions around the active site histidine57 and serine195 of drosophila melanogaster alpha trypsin, including aspartate189 of the specificity pocket. these primers amplified three transcripts (sip1-3) from midguts of s. incertulas, and two transcripts (hap1-2) ... | 2000 | 10646971 |
| [identification of cry-type genes of 31 bacillus thuringiensis isolates and analysis of their expression product]. | in this study, cry-type gene of 31 isolates of bt had been identified using pcr-rflp identification system, and sds-page analysis and bioassay had been performed. 25 strains containing cry1 type gene express 130-150 kd protein and 16 of them contain cry1i gene, which codes 80 kd protein and is larvicidal to both lepidopteran and coleopteran species. 15 strains contain both cry1 and cry2 type gene; 10 strains contain unknown cry-type gene; 6 strains don't contain seven identified cry-type gene. r ... | 2000 | 12548957 |
| [molecular cloning of enhancin gene from helicoverpa armigera granulosis virus and its expression in e. coli]. | in order to provide recombinant enhancin for studying its mechanism of increasing the mortality of larvae infected by hanpv and creating new insecticide, enhancin gene from helicoverpa armigera granulosis virus was amplified by pcr technique. 2.7 kb fragment of enhancin gene was cloned into ecori/xbai site of plasmid pbluescript ks. sequence analysis revealed that enhancin gene was similar with that reported in the literature except eight nucleotides and five amino acids. thereby enhancin gene w ... | 2000 | 12548958 |
| [expression and synergism of two cry insecticidal protein genes in pseudomonas fluorescens]. | several engineered pseudomonas fluorescens(pf) strains were constructed mainly based on a pseudomonas plasmid pjms6 alpha-lac and two insecticidal crystal protein genes of bacillus thuringiensis, cry1ac and cry2aa, and the host pf strain, p303, which was with highly antifungal activity to some plant disease fungi and colonizing ability on a wide range of plants. the dna introduction was confirmed by pcr-rflp and southern blot. the 132 kd insecticidal protein was detected in ipp101 and ipp202 by ... | 2000 | 12549049 |
| spindle bodies of heliothis armigera entomopoxvirus develop in structures associated with host cell endoplasmic reticulum. | immunoelectron microscopy has shown that morphogenesis of spindle bodies (sb) of heliothis armigera entomopoxvirus involves an iterative process of condensation, aggregation, and crystallization of the major constituent protein (fusolin) within the perinuclear space and endoplasmic reticulum (er) of infected cells and in vesicles derived from er constituents. the er-specific chaperone bip has been observed to be associated with developing sbs at all stages of this process, and it is postulated t ... | 2000 | 10753594 |
| helicoverpa armigera granulovirus interference with progression of h. zea nucleopolyhedrovirus disease in h. zea larvae. | capsular proteins from helicoverpa armigera granulovirus (hagv) have previously been shown to enhance h. armigera nucleopolyhedrovirus (hasnpv) infection in h. armigera larvae. yet, hagv and has-npv, as viable viruses, interfered with one another. in our study, we have examined the effects of co-infection of the slow-killing virus hagv with the fast-killing virus helicoverpa zea npv (hzsnpv) on h. zea larvae. the mortality parameter measured was survival time. virus stocks had 50% lethal concent ... | 2000 | 10772322 |
| the interactions between soybean trypsin inhibitor and delta-endotoxin of bacillus thuringiensis in helicoverpa armigera larva. | no significant difference in larval mortality was observed when a sublethal dose of bacillus thuringiensis (bt) var. kurstaki hd-1 crystal was supplemented with soybean trypsin inhibitor (sti) in the artificial diet fed to helicoverpa armigera in the laboratory, but supplementing a nonlethal dose of crystal with sti in the diet led to a pronounced reduction of larval growth. this concentration of crystal and two lower concentrations of sti alone had no significant effects on larval growth. the r ... | 2000 | 10843832 |
| reverse transcription of a naturally occurring nonretroviral rna produces a precise deletion in the majority of its cdna products. | a precise, reproducible deletion made during in vitro reverse transcription of rna2 from the icosahedral positive-stranded helicoverpa armigera stunt virus (tetraviridae) is described. the deletion, located between two hexamer repeats, is a 50-base sequence that includes one copy of the hexamer repeat. only the moloney murine leukemia virus reverse transcriptase and its derivative superscript i, carrying a deletion of the carboxy-terminal rnase h region, showed this response, indicating a templa ... | 2000 | 10868914 |
| enhancement in activity of homologous and heterologous baculoviruses infectious to beet armyworm (lepidoptera: noctuidae) by an optical brightener. | the nuclear polyhedrosis virus (npv) from the beet armyworm, spodoptera exigua (hübner) (semnpv), was the most active virus tested against the beet armyworm (lc50 = 4.1 pibs/mm2), followed by nuclear polyhedrosis viruses from the alfalfa looper, autographa californica (speyer) (acmnpv; lc50 = 92.6 pibs/mm2), and the celery looper, anagrapha falcifera (kirby) (afmnpv; lc50 = 195.7 pibs/mm2). in the case of the nuclear polyhedrosis virus from the bollworm, helicoverpa armigera (hübner), lc50s coul ... | 2000 | 10902301 |
| molecular and insecticidal characterization of a bacillus thuringiensis strain isolated during a natural epizootic. | a new bacillus thuringiensis strain belonging to the serovar aizawai was isolated from a dead larva of the lepidopteran mythimna loreyi collected in a corn crop in spain during a natural epizootic. this strain, which was named leapi01, was compared with the kurstaki and aizawai strains isolated from dipel(r) and xentari(r), by electron microscopy, sds-page, plasmid pattern, pcr and insecticidal activity. this strain showed similar morphological and biochemical characteristics to the standard str ... | 2000 | 10971764 |
| plant-toxin interactions in transgenic bt cotton and their effect on mortality of helicoverpa armigera (lepidoptera: noctuidae). | in australia, transgenic cotton plants expressing the cry1ac gene from bacillus thuringiensis berliner variety kurstaki are less toxic to first-instar helicoverpa armigera (hübner) after the plant is producing fruit. we developed two bioassay methods (leaf mush, leaf disk) to test if the physiological state of the plants explained changes in toxicity and a third method (diet incorporation) was developed to quantify the toxicity of bt leaves when mixed in chickpea diet. cry1ac protein was less to ... | 2000 | 10985045 |
| virus morphogenesis of helicoverpa armigera nucleopolyhedrovirus in helicoverpa zea serum-free suspension culture. | helicoverpa armigera single nucleopolyhedrovirus (hasnpv) replication in helicoverpa zea serum-free suspension culture was studied in detail and the sequence of virus morphogenesis was determined by transmission electron microscopy. by 16 h post-infection (p.i.), virus replication was observed in the virogenic stroma by the appearance of nucleocapsids. polyhedron formation was detected by 24 h p.i. and the polyhedron envelope (pe) was completely formed by 72 h p.i. pe morphogenesis of hasnpv is ... | 2000 | 10993944 |
| field evaluation of a slow release pheromone formulation to control the american bollworm, helicoverpa armigera (lepidoptera: noctuidae) in pakistan. | trials to control the american bollworm, helicoverpa armigera (hübner) by the technique of mating disruption were carried out in the pakistan province of punjab during the 1996 cotton season. a slow release pvc resin formulation, selibate(r) ha, containing a 97:3 mixture of the major (9z-hexadecenal) and minor (11z-hexadecenal) components of the female sex pheromone of h. armigera was applied at a rate of 40 g active ingredient ha-1 during august 1996. the formulation was applied around the term ... | 2000 | 10996859 |
| population structure of the pestiferous moth helicoverpa armigera in the eastern mediterranean using rapd analysis. | the genetic structure of the cotton bollworm, helicoverpa armigera (hübner) (lepidoptera: noctuidae), was studied in the eastern mediterranean. moths were sampled in six locations (five in israel, and one in turkey) and their genetic relationship was analysed using rapd-pcr. three 10-oligonucleotide primers revealed 84 presumptive polymorphic loci that were used to estimate population structure. results reveal low level of genetic distances among israeli and turkish populations. the estimated va ... | 2000 | 11012728 |
| biological and biochemical relationships between the nucleopolyhedroviruses of mamestra brassicae and heliothis armigera. | the multiply embedded nucleopolyhedroviruses (mnpv) originally isolated from mamestra brassicae (german and dutch isolates) and heliothis armigera have been studied comparatively to establish their relatedness, both in terms of biological activity and genomic homology. all three viral isolates replicated in m. brassicae, h. armigera, heliothis zea, and heliothis virescens, resulting in each case in progeny virus that was essentially similar to the inoculum. dose-mortality studies carried out on ... | 2000 | 10631051 |
| molecular cloning and expression of cyp6b8: a xanthotoxin-inducible cytochrome p450 cdna from helicoverpa zea. | xanthotoxin, a plant allelochemical, induces alpha-cypermethrin insecticide tolerance in helicoverpa zea (corn earworm); inhibition of tolerance by piperonyl butoxide implicates cytochrome p450 monooxygenases (p450s) in the detoxification of this insecticide. to characterize the xanthotoxin-inducible p450 that might mediate alpha-cypermethrin tolerance in this species, a cdna library prepared from xanthotoxin-induced h. zea fifth instar larvae was screened with cdnas encoding furanocoumarin-meta ... | 2000 | 10646973 |
| [the characteristics of bacillus thuringiensis strain ybt833 and its transformants that containing different icp genes]. | four different transformants were selected by transferring cry1c into bacillus thuringiensis strain ybt833. southern blot and plasmid profiles results all proved that cry1c was transferred into strain ybt833. however, it was found by pcr analysis that transformant ybt833-1 kept all indigenous icp(insecticidal crystal protein) genes of strain ybt833 while transformant ybt833-2 lost cry1ab, and transformant ybt833-3 lost all icp genes. sds-page showed that transformants of ybt833-1, ybt833-2 and y ... | 2000 | 11132501 |
| effect of two granulosis viruses on the activity of the gypsy moth (lepidoptera: lymantriidae) nuclear polyhedrosis virus. | two granulosis viruses (gv) were tested as enhancers for the gypsy moth nuclear polyhedrosis virus (ldmnpv). helicoverpa armigera (hübner) cv (hagv) had no detrimental effect upon larval growth and development, but in combination with ldmnpv it reduced both the lc50 and the lt50 for the npv. in addition, the combination also adversely affected the growth and development of gypsy moth larvae. the lc50 of ldmnpv was reduced by as much as 300-fold (hagv at 10(-2) dilution) and the lt50 was reduced ... | 2000 | 11142292 |
| [transfer of cry1c gene into bacillus thuringiensis by electroporation to construct strain with broader insecticidal activity]. | three transformants were selected by transferring cry1c into bacillus thuringiensis strain ybt1535. plasmid profiles, pcr and southern blot result all proved that cry1c had been transferred into strain ybt1535. bioassay results showed that the transformants of strain ybt1535 displayed significantly higher toxicity against spodoptera exigua than strain ybt1535, but the toxicities against heliothis armigera and plutella xylostella did not rise except transformant ybt1535-2. | 2000 | 11191763 |
| [expression of 2.1 kb enhancin gene fragment from helicoverpa armigera granulosis virus in escherichia coli]. | the 2.1 kb fragment of enhancin gene from helicoverpa armigera granulosis virus was inserted into vector pqe-30 and expressed successfully in e. coli m15(prep4). the synergy of expression product(p78) on acmnpv against the larvae of plutella xylostella was also studied. the results indicated that the percentage of correct mortality of the larvae increased 27.88%-32.92% in 10 post-infection days. | 2000 | 11191765 |
| growth inhibitory and antifeedant activity of extracts from melia dubia to spodoptera litura and helicoverpa armigera larvae. | the growth inhibitory activity and deterrency of melia dubia (meliaceae) extracts to spodoptera litura and helicoverpa armigera were investigated. artificial diet bioassays using neonate larvae of both s. litura and h. armigera indicated that dichloroethane (dce) and methanol (me) extracts of m. dubia inhibited growth in a dose dependent manner. dce and me-5ii fractions also resulted in 50% deterrency at concentrations of 22.5 and 16.8 micrograms/cm2 respectively against s. litura larvae in a le ... | 2000 | 11233088 |
| both lymantria dispar nucleopolyhedrovirus enhancin genes contribute to viral potency. | enhancins are a group of proteins first identified in granuloviruses (gv) that have the ability to enhance nuclear polyhedrosis virus potency. we had previously identified an enhancin gene (e1) in the lymantria dispar multinucleocapsid nucleopolyhedrovirus (ldmnpv) (d. s. bischoff and j. m. slavicek, j. virol. 71:8133-8140, 1997). inactivation of the e1 gene product within the viral genome lowered viral potency by an average of 2.9-fold. a second enhancin gene (e2) was identified when the entire ... | 2001 | 11507209 |
| [studies on transgenic tobacco plants expressing two kinds of insect resistant genes]. | a synthetic bt cry1ac gene fussed with a secretary signal coding sequences at 5' end and a modified gna gene were used to construct a plant expression vector pbsgs1m+ and this vector was transferred into tobacco (nicotiana tabacum l.) by agrobacterium-mediated transformation method. results of pcr, southern blot and slot blot analysis indicated that both the chimeric bt cry1ac and gna genes were integrated into the genomes of transformed plants. western blot analysis indicated that at least the ... | 2001 | 11517599 |
| replication-independent assembly of an insect virus (tetraviridae) in plant cells. | infectious virions of the insect rna virus helicoverpa armigera stunt virus (hasv; omegatetravirus, tetraviridae) were assembled in cultured plant protoplasts of nicotiana plumbaginifolia in the absence of detectable replication. assembly of the virus, which has not been grown in cell culture, required cotransfection of a dna plasmid expressing the hasv capsid gene in combination with either genomic rna or with dna plasmids carrying the complete cdnas to the two hasv genomic rnas. each cdna was ... | 2001 | 11543656 |
| immunolocalization of odorant-binding proteins in noctuid moths (insecta, lepidoptera). | odorant-binding proteins were studied in the noctuid moths agrotis segetum, autographa gamma, helicoverpa armigera, heliothis virescens and spodoptera littoralis using antisera raised against the pheromone-binding protein (pbp) and general odorant-binding protein 2 (gobp2) of antheraea polyphemus (saturniidae). proteins immunoreacting with these antisera were only found on the antennae and pbp and gobp2 could be identified on western blots of males and females of all five species. pbps were pred ... | 2001 | 11555483 |
| aminopeptidase-n from the helicoverpa armigera (hubner) brush border membrane vesicles as a receptor of bacillus thuringiensis crylac delta-endotoxin. | brush border membrane vesicles (bbmvs) were prepared from the 2nd instar larvae of helicoverpa armigera. binding of the activated cry1ac of bacillus thuringiensis (bt) toxin was shown by immunoblot. a 120-kda protein was identified as a receptor for the cry1ac type delta-endotoxin. the aminopeptidase-n activity of bbmvs was measured as the hydrolysis of l-leucine p-nitroanilide. the specific activity was 35 units/mg protein. the bbmv preparation also showed low level of alkaline phosphatase acti ... | 2001 | 11683359 |
| effects of the 20-kilodalton helper protein on cry1ac production and spore formation in bacillus thuringiensis. | bacillus thuringiensis produces large amounts of various pesticidal proteins during the stationary phase. in order to achieve a high yield and form crystals, some pesticidal proteins require the presence of other proteins. helper protein p20 is required for efficient production of both the cyt1a and cry11a crystal proteins in b. thuringiensis subsp. israelensis. although full-length cry1 protoxins are usually independent in terms of expression and crystallization in b. thuringiensis, in this stu ... | 2001 | 11722880 |