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deleterious effect of suboptimal diet on rest-activity cycle in anastrepha ludens manifests itself with age.activity patterns and sleep-wake cycles are among the physiological processes that change most prominently as animals age, and are often good indicators of healthspan. in this study, we used the video-based high-resolution behavioral monitoring system (bms) to monitor the daily activity cycle of tephritid fruit flies anastrepha ludens over their lifetime. surprisingly, there was no dramatic change in activity profile with respect to age if flies were consistently fed with a nutritionally balance ...023639915
sterility in the mexican fruit fly caused by chemicals.chlorambucil, 4-{p-[bis(2-chloroethyl) amino] phenyl} butyrate, administered in food to the mexican fruit fly anastrepha iudens (loew) inhibits the growth of testes and reduces egg hatch to almost nothing. oviposition is not affected. the compound 4-amino-1h-pyrazolo (3.4-d) pyrimidine sulfate inhibits the growth of ovaries and reduces oviposition and fertility.196217732194
laboratory techniques for evaluating hempa and other chemosterilants against the mexican fruit fly. 19665976114
behavioral responses of male and female mexican fruit flies,anastrepha ludens, to male-produced chemicals in laboratory experiments.the behavioral responses of male and female mexican fruit flies elicited by male abdominal extracts were measured in laboratory cages where pheromone was applied to the undersides of some leaves on a treated tree but to none of the leaves on a control tree. after arrival to the treated tree, females came directly to pheromone sources. females on the treated tree visited leaves and fought other females at higher rates than on the control tree. females stayed on treated leaves and trees longer tha ...198624306395
behavioral responses of female mexican fruit flies,anastrepha ludens, to components of male-produced sex pheromone.the behavioral responses of virgin female mexican fruit flies elicited by components and combinations of the components of male-produced pheromone were measured in a laboratory wind-tunnel bioassay where test chemicals were applied to the undersides of some leaves on a treated tree but to none of the leaves of a control tree. only treatments containing at least (z)-3-nonenol and/or (z,z)-3,6-nonadienol in combination with (s,s)-(-)-epianastrephin elicited strong behavioral responses. responses i ...198824276529
responses of laboratory-strain mexican fruit flies,anastrepha ludens, to combinations of fermenting fruit odor and male-produced pheromone in laboratory bioassays.the attractiveness toa. ludens adults of a combination of odor of fermented chapote fruit and pheromone was compared to attractiveness of both chapote and pheromone in laboratory bioassays. the chemical treatments were tested on 2-day-old and 10- to 12-day-old (sexually immature and mature, respectively), virgin and mated, nonstarved and starved, males and females during the early afternoon when flies were sexually inactive and the late afternoon when sexually mature flies were sexually active. ...199024264004
a novel attractant for mexican fruit fly,anastrepha ludens, from fermented host fruit.chemicals from fermented chapote fruit were identified and evaluated as attractants for hungry adult mexican fruit flies in laboratory and greenhouse bioassays. twenty-eight chemicals identified from an attractive gas-chromatography fraction were as attractive as a chapote volatiles extract (cv) when mixed in the same amounts found in cv. sixteen of the chemicals were slightly attractive to flies when tested individually. a mixture containing 15 of the chemicals by design and the 16th as an impu ...199024263255
a four-component attractant for the mexican fruit fly,anastrepha ludens (diptera: tephritidae), from host fruit.sixteen chemicals found in fermented chapote fruit odor were evaluated as attractants for hungry adult mexican fruit flies. ethyl octanoate, ethyl benzoate, terpinyl acetate, ethyl salicylate, and (-)-α-copaene proved slightly attractive. several of the chemicals also were tested for their ability to increase the attractiveness of the previously developed chapote-derived attractant (ceh) consisting of 1,8-cineole, ethyl hexanoate, and hexanol. combinations containing ceh with ethyl octanoate, et ...199224254162
attraction of both sexes of mexican fruit fly,anastrepha ludens, to a mixture of ammonia, methylamine, and putrescine.eight chemicals were evaluated in laboratory experiments as attractants for sugar-fed adult mexican fruit flies. ammonium bicarbonate, methylamine hcl, ethanolamine, pyrrolidine, putrescine, and monomethyl succinate were slightly attractive when tested singly. a mixture containing all eight chemicals was much more attractive than any of the individual chemicals. through a series of experiments, a mixture of three of the chemicals was found that was at least as attractive as the original eight-co ...199324248791
partial characterization and hplc isolation of bacteria-produced attractants for the mexican fruit fly,anastrepha ludens.methods were developed to collect and isolate volatile chemicals produced by astaphylococcus bacterium in tryptic soy culture that are attractive to protein-hungry adult mexican fruit flies. centrifugation of bacteria culture yielded a slightly attractive pellet containing most of the bacteria cells and a highly attractive supernatant. supernatant filtered to remove the remaining bacteria was as attractive as the unfiltered supernatant. filtrate at ph 7 and above was much more attractive than fi ...199324248955
attractants fromstaphylococcus aureus cultures for mexican fruit fly,anastrepha ludens.volatile chemicals from tryptic soy broth cultures ofstaphylococcus aureus that attract sugar-fed, protein-hungry adult mexican fruit flies were identified. chemicals identified from the headspace above the filtrate of the bacterial cultures were ammonia, trimethylamine, isoamylamine, 2-methylbutylamine, 2,5-dimethylpyrazine, and acetic acid. each chemical attracted flies. a mixture of the chemicals in the same concentrations as were found in the bacterial filtrate was 89% as effective in attrac ...199524233835
attractiveness to mexican fruit flies of combinations of acetic acid with ammonium/amino attractants with emphasis on effects of hunger.ammonium acetate was more attractive than other ammonium salts to mexican fruit flies (anastrepha ludens) in an orchard test. we hypothesized that acetic acid enhanced the attractiveness of ammonia in the orchard test and that acetic acid may similarly enhance attractiveness of ampu, an attractant consisting of a mixture of ammonium bicarbonate or ammonium carbonate, methylamine hcl, and putrescine. in laboratory experiments, acetic acid was attractive to flies deprived of either yeast hydrolysa ...199624227489
toxicity of an isolate of bacillus thuringiensis subspecies darmstadiensis to adults of the mexican fruit fly (diptera: (diptera:tephritidae) in the laboratory.centrifugation pellets obtained from an isolate of bacillus thuringiensis subspecies darmstadiensis (guat 1) cultured from a guatemalan soil sample were found to be toxic to anastrepha ludens (loew) adults in the laboratory. we developed a bioassay diet that consisted of a mixture of the bacterium, a protein source, and sugar. a ph of 4.1 of the mixture was needed to obtain maximum adult mortality. one meal of the diet, which lasted from 30 s to 4 min, was enough to cause > 70% mortality of both ...19979071889
toxicity of bacillus thuringiensis beta-exotoxin to three species of fruit flies (diptera: tephritidae).the current study describes toxic effects of the bacillus thuringiensis beta-exotoxin toward 3rd instars of 3 fruit fly species: anastrepha ludens (loew), a. obliqua (macquart), and a. serpentina (wiedemann). the beta-exotoxin was highly toxic to all 3 species tested, with lc50 values calculated as 0.641, 0.512, and 0.408 microgram/cm2 of filter paper used to expose the larvae, for a. ludens, a. obliqua, and a. serpentina, respectively. exposure to beta-exotoxin was associated with an increase i ...199910582044
lack of toxicity to adults of the mexican fruit fly (diptera: tephritidae) of beta-exotoxin in bacillus thuringiensis endotoxin preparations.beta-exotoxin (thuringiensin) was found in high titers in centrifugation supernatants and acetone/lactose powders produced from centrifugation pellets of strains guat 1 and hd 2 of bacillus thuringiensis (berliner). diets containing powders of either strain were toxic, diets containing guat 1 supernatant were not toxic, diets containing hd 2 supernatant were slightly toxic, and diets containing powders or supernatants from uninoculated culturing medium spiked with beta-exotoxin were not toxic. m ...200010985014
virulence of metarhizium anisopliae (deuteromycotina: hyphomycetes) on anastrepha ludens (diptera: tephritidae): laboratory and field trials.twenty isolates of the fungus metarhizium anisopliae (metsch). sorkin (ma) were evaluated to determine their virulence against last instar and adult emergence of mexican fruit fly, anastrepha ludens (loew). larvae were exposed by immersion in a conidial suspension at a concentration of 10(8) ufc/ml under laboratory conditions. larvae and pupae cumulative mortality rates ranged from 37.9 to 98.75%. thirteen isolates caused mortality rates > 83.7%, and their lt50 values ranged from 1.8 to 6.2 d. t ...200010985015
heating rate and induced thermotolerance in mexican fruit fly (diptera: tephritidae) larvae, a quarantine pest of citrus and mangoes.a bioassay and graduated temperature water baths were used to document the induction of thermotolerance in third-instar mexican fruit fly, anastrepha ludens (loew). the 99% lethal time dose for larvae exposed to 44 degrees c core temperatures in artificial fruit is 61.5 min when a slow heating rate (120 min ramp) is applied, but only 41.9 min when a fast heating rate (15 min ramp) is applied. in electrophoretic profiles a heat inducible protein of molecular weight 32 kda was detected in 76% of t ...200010985055
a new basal subfamily of mariner elements in ceratitis rosa and other tephritid flies.several copies of highly related transposable elements, crmar2, almar1, and asmar1, are described from the genomes of ceratitis rosa, anastrepha ludens, and a. suspensa, respectively. one copy from c. rosa, crmar2.5, contains a full-length, uninterrupted orf. all the other copies, from the three species contain a long deletion within the putative orf. the consensus crmar2 element has features typical of the mariner/tc1 superfamily of transposable elements. in particular, the crmar2 consensus enc ...200111677619
photoactive dye insecticide formulations: adjuvants increase toxicity to mexican fruit fly (diptera: tephritidae).the efficacy of photo active dyes as insecticides depends on the ingestion of the dye by the target insect and the activity of the dye at sensitive sites or on essential chemical functions. the site of this activity in insects is not understood, but we have found that certain chemical additives enhance the toxicity of phloxine b in the mexican fruit fly. a series oftests with commercial adjuvants was performed under laboratory conditions that demonstrated a multifold decrease in the ld50 of phlo ...200111233106
phylogenetic relationships among fruit flies, bactrocera (diptera, tephritidae), based on the mitochondrial rdna sequences.nucleotide sequences of a 1.6 kb long portion of the mitochondrial dna containing the majority of the 16s rrna gene, the trnaval gene, and the 5' half-region of the 12s rrna gene were determined for forty-eight individuals of nineteen bactrocera species and one other tephritid taxon, anastrepha ludens. phylogenetic analyses were performed using the consistently aligned 1.5 kb long sequences, excluding seventeen portions that could not be aligned unambiguously and were aligned inconsistently amon ...200111903624
effects of age, diet, female density, and the host resource on egg load in anastrepha ludens and anastrepha obliqua (diptera: tephritidae).oocyte counts, used as a measure of egg load, were compared among three different age groups (15, 30 and 45 days) of two polyphagous species of tephritid fruit flies, anastrepha ludens and anastrepha obliqua, which were exposed to varying conditions of diet (sucrose vs sucrose and protein), availability of oviposition substrate (present vs absent), adult female density (1, 2 and 4 females/cage), and semiochemical context (presence vs absence of male pheromones and fruit volatiles). in both speci ...200111472760
isolation and identification of bacteria associated with adult laboratory mexican fruit flies, anastrepha ludens (diptera: tephritidae).from the guts of new and old colonies (female and male) of mexican fruit flies, anastrepha ludens (diptera: tephritidae), we identified a total of 18 different bacterial species belonging to the family enterobacteriaceae, pseudomonadaceae, vibrionaceae, micrococcaceae, deinococcacea, bacillaceae, and the genus listeria. enterobacter, providencia, serratia, and staphylococcus spp. were the most frequently isolated genera, with citrobacter, streptococcus, aerococcus, and listeria found less freque ...200111178731
field evaluation of a phototoxic dye, phloxine b, against three species of fruit flies (diptera: tephritidae).the xanthene dye phloxine b (d&c red #28) bait was sprayed against fruit flies in mango orchards in 1996 and 1997. the flies used for testing were mexican fruit fly, anastrepha ludens (loew), west indian fruit fly, anastrepha obliqua (macquart), and mediterranean fruit fly, ceratitis capitata (wiedeman). results of the experiments indicate that the toxic efficacy of phloxine b against these fruit flies is as good as that of malathion-bait sprays. results also indicate that type of protein used w ...200111777044
beauveria bassiana as a pathogen of the mexican fruit fly (diptera: tephritidae) under laboratory conditions.bioassays were carried out under controlled conditions (27 +/- 2 degrees c, 80 +/- 5% rh, and a photoperiod of 12:12 [l:d] h) to evaluate the effect of eight strains of the entomopathogenic fungus beauveria bassiana upon larvae, pupae, and adult females of the mexican fruit fly, anastrepha ludens (loew). mortality of the immature stages was low, 2-8% in larvae and 0% in pupae. however, very high levels of mortality were obtained for adult flies, with values of 100, 98, and 98% for the strains bb ...200211942762
purine metabolizing capability of enterobacter agglomerans affects volatiles production and attractiveness to mexican fruit fly.we investigated two strains of enterobacter agglomerans that differ in their ability to metabolize uric acid for (1) attractiveness to sugar-fed mexican fruit flies, and (2) production of volatile chemicals that may be responsible for the attractiveness. the two strains were cultured on a medium that contained uric acid as the primary nitrogen source to simulate bird feces, a natural substrate for this bacterium. active cultures of both strains were more attractive than uninoculated uric acid me ...200212371809
age estimation of mexican fruit fly (diptera: tephritidae) based on accumulation of pterins.a common method of aging adult flies, fluorescence spectrometry, was used to monitor the increase of overall pterine titer in head extracts of anastrepha ludens (loew). accumulation of fluorescent compounds was measured as a function of chronological age of flies maintained at 17 and 27 degrees c. although relative fluorescence increased with age, field studies revealed that this phenomenon could not be used for accurate age estimation, as relative fluorescence did not increase predictably with ...200212539849
composition of the surface hydrocarbons from the vitelline membranes of dipteran embryos.hydrocarbons were the major lipid class extracted by hexane from the vitelline membrane surface of dechorionated eggs of the house fly, musca domestica, the new world screwworm, cochliomyia hominivorax, the secondary screwworm, cochliomyia macellaria, the green bottle fly, phaenicia sericata, the sheep blow fly, lucilia cuprina and the mexican fruit fly, anastrepha ludens. the length of time the embryos must be exposed to hexane with or without a small amount of alcohol in order to attain permea ...200314529755
adult diet and male-female contact effects on female reproductive potential in mexican fruit fly (anastrepha ludens loew) (diptera tephritidae).wild strains of fruit flies (diptera: tephritidae) placed into laboratory rearing conditions are subjected to selection pressures caused by the diet, cages, density of flies, and other factors. selection that changes mating behavior of the strain may result in less effective males released in sterile insect programs. tests were performed to examine the effects of protein in diet and adult interactions on egg production and mating during sexual maturation of the mexican fruit fly (anastrepha lude ...200314994799
nonhost status of citrus sinensis cultivar valencia and c. paradisi cultivar ruby red to mexican anastrepha fraterculus (diptera: tephritidae).anastrepha fraterculus (wiedemann) is recognized as a pest of citrus, apples, and blackberries in south america. in mexico, it is mainly found in fruit of the family myrtaceae and has never been reported infesting citrus. here, we sought to determine whether females stemming from mexican a. fraterculus populations (collected in the state of veracruz) would lay eggs in 'valencia' oranges and 'ruby red' grapefruit and, if so, whether larvae would hatch and develop. we worked under laboratory and s ...200314977105
nonhost status of commercial persea americana 'hass' to anastrepha ludens, anastrepha obliqua, anastrepha serpentina, and anastrepha striata (diptera: tephritidae) in mexico.the objective of this study was to determine the host status in mexico of commercially cultivated and marketed avocado, persea americana (mill.), 'hass' to anastrepha ludens (loew), anastrepha obliqua (macquart), anastrepha serpentina (wiedemann), and anastrepha striata (schiner) (diptera: tephritidae). experiments in michoacán, mexico, were carried out in six orchards located at three altitudes above sea level during two times (august-october 2001 and april-june 2002). they included choice ('ha ...200415154448
volatiles production and attractiveness to the mexican fruit fly of enterobacter agglomerans isolated from apple maggot and mexican fruit flies.we investigated two strains of uricase (+) enterobacter agglomerans, one isolated from the apple maggot fly (amf) and one from the mexican fruit fly (mff), for 1) attractiveness to mff, and 2) production of attractive chemicals. regarding chemicals demonstrated attractive to the mff, the mff bacterial strain produced more 2,5-dimethylpyrazine, 2-phenylethanol, and indole than the amf strain, whereas the amf, but not the mff strain, produced 3-hydroxybutanone. cell types that predominated in plat ...200415503523
efficacy of 1,4-diaminobutane (putrescine) in a food-based synthetic attractant for capture of mediterranean and mexican fruit flies (diptera: tephritidae).field trials were conducted in guatemala to evaluate the importance of 1,4 diaminobutane (putrescine) in traps baited with ammonium acetate, trimethylamine, and putrescine. for the mediterranean fruit fly, ceratitis capitata (wiedemann), there were no differences in percentage of females captured in coffee and citrus or in percentage of males captured in citrus in traps with ammonium acetate and trimethylamine lures (females in coffee, 26.4 +/- 6.27%; females in citrus, 35.7 +/- 5.35%; males in ...200415279300
irradiation of mangoes as a postharvest quarantine treatment for fruit flies (diptera: tephritidae).mangoes infested with third instar larvae were irradiated using co-60 gamma rays and a dose interval of 2-250 gy to assess the irradiation dose required to prevent adult emergence of the mexican fruit fly (anastrepha ludens), the west indies fruit fly (a. obliqua), the sapote fruit fly (a. serpentina), and the mediterranean fruit fly (ceratitis capitata). doses of 76.9, 87.3, 91.4 and 112.7 gy, were estimated to inhibit 99.9968% (probit 9) of adult emergence fora. obliqua, a. serpentina, a. lude ...200415154447
nontarget impact of spinosad gf-120 bait sprays for control of the mexican fruit fly (diptera: tephritidae) in texas citrus.bait sprays containing the toxicant spinosad (gf-120) were applied to citrus groves in the rio grande valley of texas where mexican fruit flies were detected in surveillance traps. the sprays were applied as a supplement to a continuous sterile insect release program. sterile fly captures were 47-63% lower in the treated groves compared with control groves. eight of 10 secondary pest populations declined in the test groves subsequent to spray applications, but they also declined in the control g ...200516539119
pupariation time as a source of variability in mating performance in mass-reared anastrepha ludens (diptera: tephritidae).the effect of time of pupariation on pupal weight and adult sexual competitiveness under field cage conditions was studied in mass-reared anastrepha ludens (loew) males. larvae that took 72 h to pupariate after separation from diet resulted in lighter pupae than those that took 24 and 48 h. wild pupae were heavier than the 48- and the 72-h pupae but not the 24-h pupae. interestingly, no differences in mating performance were found between males of the 24- and 48-h pupae despite differences in pu ...200516539116
reaction orders for thermal mortality of third instars of mexican fruit fly (diptera: tephritidae).mexican fruit fly, anastrepha ludens (loew), is a quarantine pest of several fruit, including citrus, avocados, and mangoes, from extreme southern texas to costa rica. to provide information for modeling heat phytosanitary treatments, third instars were heated with an aluminum heating block between 44 and 50 degrees c for time intervals up to those causing 100% mortality. at 44 and 50 degrees c, 100% mortality was achieved at 100 and 2 min, respectively. each 2 degrees c increase in temperature ...200516539112
efficacy of two synthetic food-odor lures for mexican fruit flies (diptera: tephritidae) is determined by trap type.sterile mass-reared mexican fruit flies, anastrepha ludens (loew), were trapped in a citrus orchard by using multilure traps and cylindrical sticky traps baited with advanced pheromone technologies anastrepha fruit fly (aff) lures or suterra biolure two-component (ammonium acetate and putrescine) mff lures (biolures). the cylinder trap/aff lure combination was the best trap over the first 6 wk, the multilure trap/biolure combination was best during weeks 6-12, and the multilure trap/aff lure com ...200516334319
mass rearing history negatively affects mating success of male anastrepha ludens (diptera: tephritidae) reared for sterile insect technique programs.mating competitiveness and sterility induction into cohorts of wild anastrepha ludens (loew) (diptera: tephritidae) was compared among wild and laboratory flies reared for use in the sterile insect technique mexican program. laboratory flies stemming from an 11-yr-old bisexual strain were either not irradiated, irradiated at 3 krad (low dose), or irradiated at 8 krad. in 30 by 30 by 30-cm plexiglas cages, where a cohort of laboratory flies (male and female) irradiated at different doses (0, 3, a ...200516334318
grapefruit oil enhances attraction of mexican fruit flies to a synthetic food-odor lure.we investigated the attractiveness of grapefruit oil to the mexican fruit fly. only high concentrations were attractive in laboratory wind-tunnel bioassays. attraction of flies to grapefruit oil was not enhanced if they had previous experience with grapefruit. in citrus orchard experiments, undiluted grapefruit oil attracted mexican fruit flies and enhanced attraction to traps baited with a synthetic food-odor lure emitting ammonia and other nitrogenous chemicals. this is the first demonstration ...200516124231
biodemography of a long-lived tephritid: reproduction and longevity in a large cohort of female mexican fruit flies, anastrepha ludens.age of sexual maturity, daily and lifetime reproductive rates, and life span were recorded in a laboratory cohort of mexican fruit flies consisting of over 1100 females maintained individually. the results revealed that, relative to the medfly, the mexfly is slower maturing (14 vs 17 days), more fecund (1400 vs 650-1100 eggs/female), and longer lived (50 vs 35 days). the results reinforced the generality of several earlier findings on the medfly including the deceleration of mortality at older a ...200516154309
a new blend of white sapote fruit volatiles as potential attractant to anastrepha ludens (diptera: tephritidae).the behavioral and electrophysiological responses of nonirradiated male and female anastrepha ludens (loew) (diptera: tephritidae), to white sapote, casimiroa edulis oerst. (rutaceae), volatiles were investigated. females flew upwind and landed more often on fruit than on artificial fruit in wind tunnel bioassays. males flew upwind (but not landed) more frequently on fruit than on artificial fruit. porapak q volatile extracts of white sapote also elicited upwind flight and landing on artificial ...200617195665
foraging behavior of anastrepha ludens, a. obliqua, and a. serpentina in response to feces extracts containing host marking pheromone.following oviposition, females of many tephritid flies deposit host marking pheromones (hmps) to indicate that the host fruit has been occupied. we describe the foraging behavior of these three economically important species (anastrepha ludens and a. obliqua from the fraterculus species group and a. serpentina from the serpentina species group) when they encounter an artificial fruit (green agar spheres wrapped in parafilm) marked with intra- and interspecific feces extracts that contain, among ...200616555132
long aculeus and behavior of anastrepha ludens render gibberellic acid ineffective as an agent to reduce 'ruby red' grapefruit susceptibility to the attack of this pestiferous fruit fly in commercial groves.treating mexican grapefruit with gibberellic acid (ga3) before color break, significantly delayed peel color change and increased peel puncture resistance, but it did not reduce grapefruit susceptibility to mexican fruit fly, anastrepha ludens (loew) attack under natural conditions. despite ga3 treatments, larval infestation levels increased with higher fruit fly populations, which also increased as the season progressed. late in the season, infestation levels were even higher in ga3-treated fru ...200616937671
comparison of sexual compatibility between laboratory and wild mexican fruit flies under laboratory and field conditions.the sexual compatibility between laboratory (lf) and wild (wf) strains of the mexican fruit fly, anastrepha ludens (loew), was analyzed using analogous methodologies and experimental arenas under both laboratory and field conditions. sexual compatibility was quantified with the following indices: the isolation index (isi), male relative performance index (mrp), female relative performance index (frpi), and the relative sterility index (rsi). isi detected a certain level of incompatibility betwee ...200617195663
horizontal transmission of beauveria bassiana in anastrepha ludens (diptera: tephritidae) under laboratory and field cage conditions.the virulence of two products of the fungus beauveria bassiana (lcpp and bassianil) on adult anastrepha ludens (loew) (diptera: tephritidae) and their effect on the mating performance of infected males was evaluated in laboratory and field cage tests. the horizontal transmission capacity of the fungus during copulation or attempted copulation also was quantified using inoculated males as well as the impact of infection on female fecundity and longevity. both fungal products were found to be high ...200717461049
development of bait stations for fruit fly population suppression.the application of insecticides is an essential component for eradication or management of fruit fly pests. impact on nontarget organisms and public rejection of areawide pesticide applications have been major concerns in managing these programs. bait stations have been proposed as alternative treatments in areas where broadcast insecticides are not acceptable. in this study, we defined bait stations as discrete containers of attractants and toxins, which are targeted at specific pests. tests we ...200717461069
comparison of two synthetic food-odor lures for captures of feral mexican fruit flies (diptera: tephritidae) in mexico and implications regarding use of irradiated flies to assess lure efficacy.feral mexican fruit flies, anastrepha ludens (loew) (diptera: tephritidae), were trapped in a citrus orchard in mexico by using two types of synthetic food-odor lures, the aff lure (anastrepha fruit fly lure, aptiv, inc., portland, or) and the biolure (two-component mff lure, suterra llc, inc., bend, or). in multilure traps (better world manufacturing, inc., miami, fl) containing water, biolures captured about the same numbers of flies as aff lures. in multilure traps containing antifreeze solut ...200717849863
irradiation of anastrepha ludens (diptera: tephritidae) revisited: optimizing sterility induction.irradiation doses currently applied to sterilize mexican fruit flies, anastrepha ludens (loew) (diptera: tephritidae), for release under the sterile insect technique eradication campaign in mexico, were reviewed in an effort to increase sterile male performance in the field. a dose maximizing sterility induction into wild populations was sought by balancing somatic fitness with genetic sterility. doses of 40, 60, and 80 gy induced 95% or more sterility in all males, which in turn induced similar ...200717849864
germ-line transformation of the mexican fruit fly.germ-line transformation of a major agricultural pest, the mexican fruit fly (anastrepha ludens loew, mexfly), was achieved using composite piggybac transposable elements marked with green, yellow and red fluorescent proteins (copgreen, phiyfp and j-red). we also investigated the possibility of generating transposon-free insertions, in order to address potential concerns relating to proposed field use of transgenic mexfly. we describe a highly efficient method for transforming mexfly, compare ef ...200717894556
colonization of a hybrid strain to restore male anastrepha ludens (diptera: tephritidae) mating competitiveness for sterile insect technique programs.to restore male mating competitiveness of mexican fruit flies, anastrepha ludens (loew) (diptera: tephritidae), reared for sterile insect releases by the mexican fruit fly eradication campaign, two strain replacement techniques were evaluated. field cage male competitiveness tests revealed that laboratory males of the metapa strain mated 3 times less often with wild females than field-collected wild males. a strain developed from the cross of wild males and laboratory females (hybrid strain) was ...200717598535
cryopreservation of mexican fruit flies by vitrification: stage selection and avoidance of thermal stress.this report presents details of a vitrification methodology for the cryopreservation of embryos of the mexican fruit fly, anastrepha ludens. the overall summary of the data indicates that selecting the correct developmental stage for cryopreservation is the most important criterion. the key aspect in selection of the correct stage is to balance depletion of the gut yolk content against development of the embryonic cuticle. embryogenesis was divided into four stages between 90 and 120 h after inc ...200717150205
longevity-fertility trade-offs in the tephritid fruit fly, anastrepha ludens, across dietary-restriction gradients.although it is widely known that dietary restriction (dr) not only extends the longevity of a wide range of species but also reduces their reproductive output, the interrelationship of dr, longevity extension and reproduction is not well understood in any organism. here we address the question: 'under what nutritional conditions do the longevity-enhancing effects resulting from food restriction either counteract, complement or reinforce the mortality costs of reproduction? to answer this questio ...200818346215
potential increase in fruit fly (diptera: tephritidae) interceptions using ionizing irradiation phytosanitary treatments.irradiation postharvest phytosanitary treatments are used increasingly and show further promise because of advantages compared with other treatments. its chief disadvantage is that, unlike all other commercially used treatments, it does not provide acute mortality, although it prevents insects from completing development or reproducing. the objective of this research was to determine to what extent irradiated egg and early instars of tephritids would develop to later instars that could be found ...200818613570
wolbachia in two populations of melittobia digitata dahms (hymenoptera: eulophidae).we investigated two populations of melittobia digitata dahms, a gregarious parasitoid (primarily upon a wide range of solitary bees, wasps, and flies), in search of wolbachia infection. the first population, from xalapa, mexico, was originally collected from and reared on mexican fruit fly pupae, anastrepha ludens loew (diptera: tephritidae); the other, from athens, georgia, was collected from and reared on prepupae of mud dauber wasps, trypoxylon politum say (hymenoptera: crabronidae). pcr stud ...200819169549
there is no magic fruit fly trap: multiple biological factors influence the response of adult anastrepha ludens and anastrepha obliqua (diptera: tephritidae) individuals to multilure traps baited with biolure or nulure.field-cage experiments were performed to determine the effectiveness of multilure traps (better world mfg inc., fresno, ca) baited with nulure (miller chemical and fertilizer corp., hanover, pa) or biolure (suterra llc, inc., bend, or) in capturing individually marked mexican fruit fly, anastrepha ludens (loew), and west indian fruit fly, anastrepha obliqua (macquart) (diptera: tephritidae), of both sexes. experimental treatments involved wild and laboratory-reared flies of varying ages (2-4 and ...200919253622
the prolongevity effect of resveratrol depends on dietary composition and calorie intake in a tephritid fruit fly.several studies have shown that resveratrol can extend lifespan in yeast, worm, fruit fly and short-lived fish, as well as mice under a high-fat diet, probably acting through molecular pathways similar to dietary restriction. however, the putative prolongevity effect of resveratrol has not been observed in other studies. to evaluate the robustness of the prolongevity effects of resveratrol, we designed a nutritional study to address the question, under what nutritional conditions does resveratro ...200919264118
the beta2-tubulin gene from three tephritid fruit fly species and use of its promoter for sperm marking.to isolate testis-specific regulatory dna that could be used in genetically transformed insect pest species to improve their biological control, beta2-tubulin genes and their proximal genomic dna were isolated from three economically important tephritid pest species, anastrepha suspensa, anastrepha ludens, and bactrocera dorsalis. gene isolation was first attempted by degenerate pcr on an a. suspensa adult male testes cdna library, which fortuitously isolated the 2.85 kb beta1-tubulin gene that ...200919520163
effects of bait age and prior protein feeding on cumulative time-dependent mortality of anastrepha ludens (diptera: tephritidae) exposed to gf-120 spinosad baits.a fruit fly bait to attract and kill adult fruit flies, gf-120, was tested in cages to determine effects of pretreatment diet and bait aging before use on cumulative mortality rates of mexican fruit fly, anastrepha ludens (loew) (diptera: tephritidae). protein-starved and protein-fed, 9-d-old flies both experienced varying overall cumulative mortality at 4, 8, 24, and 48 h. pretreatment diet had no significant effect on mortality. overall mortality rates were below 10% for 4 h, 39-43% at 8 h, bu ...200919610432
date of eclosion modulates longevity: insights across dietary-restriction gradients and female reproduction in the mexfly anastrepha ludens.we use unique experimental data on daily reproduction and survival of individual fruit flies from eight cohorts eclosed at different dates in 2004 and 2005 who were treated with varying proportions of sugar and yeast and subject to different caloric restrictions (cr). we investigate the relationship between eclosion date and longevity across diets and reproduction in anastrepha ludens. we show that eclosion date can be associated with uncontrolled external or internal factor(s) which can modulat ...200919716408
application of feces extracts and synthetic analogues of the host marking pheromone of anastrepha ludens significantly reduces fruit infestation by a. obliqua in tropical plum and mango backyard orchards.we determined the efficacy of three potential oviposition deterrents in reducing fruit infestation by anastrepha obliqua in tropical plum and mango orchards. these were: (1) extracts of feces of mexican fruit fly, anastrepha ludens, known to contain the a. ludens host marking pheromone (hmp) and (2) two fully synthetic simplified analogues of the naturally occurring compound, which we have named desmethyl a. ludens hmp (dm-hmp) and anastrephamide. two applications of feces extracts 2 or 3 wk bef ...200920069857
random mating among anastrepha ludens (diptera: tephritidae) adults of geographically distant and ecologically distinct populations in mexico.the mexican fruit fly anastrepha ludens (loew) (diptera: tephritidae) is a polyphagous pestiferous insect with a geographical range encompassing highly variable environmental conditions. considering that cryptic species have been recently found among south american representatives of the same taxonomic group as a. ludens, we tested whether or not some populations of a. ludens have evolved assortative mating as an isolating mechanism that maintains intrapopulation genetic differences and behavior ...200919063753
larval feeding substrate and species significantly influence the effect of a juvenile hormone analog on sexual development/performance in four tropical tephritid flies.the juvenile hormone (jh) analog methoprene reduces the amount of time it takes laboratory-reared anastrepha suspensa (caribbean fruit fly) males to reach sexual maturity by almost half. here, we examined if methoprene exerted a similar effect on four other tropical anastrepha species (anastrepha ludens, anastrepha obliqua, anastrepha serpentina and anastrepha striata) reared on natural hosts and exhibiting contrasting life histories. in the case of a. ludens, we worked with two populations that ...200919101560
olfactory response of the mexican fruit fly (diptera: tephritidae) to citrus aurantium volatiles.we investigated the behavioral and electrophysiological responses of male and female mexican fruit fly, anastrepha ludens (loew) (diptera: tephritidae), to volatiles of bitter orange fruit, citrus aurantium l. in field cage tests, the number of a. ludens caught in multilure traps baited with mature green bitter orange fruit was significantly higher than the number captured in traps baited with ripe yellow bitter orange fruit and control (unbaited traps). both sexes were more attracted to mature ...200919449638
male and female condition influence mating performance and sexual receptivity in two tropical fruit flies (diptera: tephritidae) with contrasting life histories.recent recognition of widespread polyandry in insects has generated considerable interest in understanding why females mate multiple times and in identifying factors that affect mating rate and inhibit female remating. however, little attention has been paid to understanding the question from both a female and male perspective, particularly with respect to factors that may simultaneously influence female remating rates. here, we report on a study aimed at ascertaining the possible interactive ef ...200919666025
leg impairments elicit graded and sex-specific demographic responses in the tephritid fruit fly anastrepha ludens.this study was concerned with the impact of different levels of artificial impairment (leg amputations) on male and female survival and female reproduction in the mexican fruit fly, anastrepha ludens. we monitored the demographic responses in a total of 100 flies of each sex that were maintained individually in 4x4x10 cm cages and subject to 1-of-11 different leg amputations (plus intact control) including cohorts in which either one front, one middle or one rear leg was severed (3 cohorts total ...200919457447
mitotic and polytene chromosome analysis in the mexican fruit fly, anastrepha ludens (loew) (diptera: tephritidae).the present study constitutes the first attempt to construct a polytene chromosome map of an anastrepha species, anastrepha ludens (loew), a major agricultural pest. the mitotic karyotype has a diploid complement of 12 acrocentric chromosomes, including five pairs of autosomes and an xx/xy sex chromosome pair. the analysis of salivary gland polytene chromosomes has shown a total number of five polytene elements that correspond to the five autosomes. the characteristic features and the most promi ...200919132068
enriching early adult environment affects the copulation behaviour of a tephritid fly.early adult experiences in enriched environments favours animal brain and behavioural development ultimately resulting in an increased fitness. however, measuring the effect of environmental enrichment in animal behaviour in nature is often a complicated task, considering the complexity of the natural environment. we expanded previous studies to evaluate how early experience in an enriched environment affects copulation behaviour when animals are confronted with a complex semi-natural environmen ...200919525439
evaluation of lufenuron as a chemosterilant against fruit flies of the genus anastrepha (diptera: tephritidae).chemosterilisation with lufenuron bait stations is a recently developed technique that is being implemented for ceratitis capitata wiedemann control. the aim of this work was to evaluate the chemosterilising effect of lufenuron against four economically important latin american fruit flies species: anastrepha ludens (loew.), a. obliqua macquart, a. serpentina wiedemann and a. striata schiner (diptera: tephritidae) in order to design a similar strategy for their control.201020157929
effect of cold storage on larval and adult anastrepha ludens (diptera: tephritidae) viability in commercially ripe, artificially infested persea americana 'hass'.commercially ripe 'hass' avocados, persea americana mill, artificially exposed to wild anastrepha ludens (loew) (diptera: tephritidae) females 24 h after harvest were placed in a cold storage facility to determine the effect of low temperature on larval survival and adult viability. fruit were left for 3, 6, 9, and 12 d in a cold room at 5 degrees c followed by a 20-25-d period at ambient temperature to allow for larval development and pupation. hass avocados and grapefruit, citrus paradisi macf ...201021309219
resistance of mexican fruit fly to quarantine treatments of high hydrostatic pressure combined with heat.high-pressure processing (hpp) has been proposed as an alternative quarantine method against the mexican fruit fly anastrepha ludens loew (diptera: tephritidae), which is one of the most important pests infesting mangoes, citrus, and other fruits in mexico and other latin-american countries. however, processing conditions used to destroy eggs and larvae also affect the shelf life of fruits. the objective of this study was to assess the biological viability of a. ludens eggs treated with hpp, est ...201020367391
efficacy of delayed atmospheric modification in a heat/modified atmosphere phytosanitary treatment.the combination of heat and low levels of oxygen increases mortality to insects infesting fruit compared with either heat or low oxygen alone. this combination treatment shows promise to disinfest commodities of quarantine pests. heated air/modified atmosphere treatments employ the modified atmosphere (e.g., low oxygen) during the entire treatment interval. there is a positive relationship between temperature and efficacy of heat/modified atmosphere treatments. efficacy of delaying atmospheric m ...201020214365
an improved quarantine method for mangoes against the mexican fruit fly based on high-pressure processing combined with heat.the mexican fruit fly anastrepha ludens loew (diptera: tephritidae) is one of the most important insects infesting mangoes, citrus, and other fruits in mexico and other latin-american countries. quarantine methods approved to destroy this insect decrease the shelf life of commodities. the objective of this study was to determine the effect of high-pressure processing using an initial temperature of 50 degrees c on the survivorship of eggs and larvae of the mexican fruit fly. eggs and larvae were ...201020001324
high hydrostatic pressure at low temperature as a quarantine treatment to improve the quality of fruits.application of high hydrostatic pressure has been proposed as an alternative quarantine process for the mexican fruit fly, anastrepha ludens loew (diptera: tephritidae), one of the most important insects infesting mangoes, citrus fruits, and other fruits in mexico and other latin american countries. the present study was performed to determine the effect of high pressure treatments at 0 degrees c on the survivorship of eggs and larvae of this pest. the effect of time and pressure level at near-f ...201019899960
host marking pheromone (hmp) in the mexican fruit fly anastrepha ludens.host marking pheromones (hmps) are used by insects to mark hosts (usually a fruit) where they have already laid eggs. the compounds serve as a deterrent to conspecifics avoiding over-infestation of hosts (i.e. repeated egg-laying into an already occupied/used host). if these hmps are sprayed onto commercially valuable fruit they act as deterrents preventing attack by females interested in laying eggs into the valuable commodity. having no insecticidal or toxic properties, and being natural produ ...201021137682
chilled packing systems for fruit flies (diptera: tephritidae) in the sterile insect technique.we evaluated three packing systems (parc boxes, "gt" screen towers and "mx" screen towers) for the emergence and sexual maturation of sterile fruit flies, at three adult fly densities (1, 1.2 and 1.3 fly/cm²) and three food types. at the lowest density, results showed no significant differences in the longevity and flight ability of adult anastrepha ludens (loew) and anastrepha obliqua macquart among the three packing systems. higher densities resulted in a decrease in these parameters. in the e ...201020877998
ionizing radiation as a phytosanitary treatment against fruit flies (diptera: tephritidae): efficacy in naturally versus artificially infested fruit.some phytosanitary irradiation treatment research against tephritid fruit flies (diptera: tephritidae) has used artificially infested fruit with the unstated and untested assumption that the method adequately simulated a natural situation. we compare grapefruit, citrus paradisi macfayden, naturally infested by mexican fruit fly, anastrepha ludens (loew), via oviposition until larvae reached the late third instar versus insertion of diet-reared third instars into holes made in grapefruits 24 h be ...201020857719
reproductive aging in tephritid fruit flies.the broad objective of this paper is to present an overview and synthesis of selected studies on reproduction and aging in two model tephritid fruit fly species including the mediterranean fruit fly, ceratitis capitata, and the mexican fruit fly, anastrepha ludens. we summarize the research findings from empirical studies and modeling investigations involving reproduction in the two tephritid species. at the end we identify and discuss four general principles regarding reproductive aging in teph ...201020738284
prolongevity effects of an oregano and cranberry extract are diet dependent in the mexican fruit fly (anastrepha ludens).botanicals have numerous health benefits. here, we used the mexican fruit fly to screen 14 compounds and botanicals for their prolongevity effects and found an oregano and cranberry mixture (oc) improved survival. we then evaluated prolongevity effects of oc within the context of diet composition. individual flies were fed 0%, 1%, or 2% oc in one of the three diets containing sugar and yeast extract (sy) at a ratio of 3:1, 9:1, or 24:1. we found that prolongevity effects of oc depended upon dose ...201019906819
host status of grapefruit and valencia oranges for anastrepha serpentina and anastrepha ludens (diptera: tephritidae).anastrepha serpentina (wiedemann) (diptera: tephritidae) is sporadically captured in the rio grande valley of texas. although its preferred hosts are in the sapotaceae family, several varieties of citrus, including grapefruit and oranges are listed as alternate hosts. although mexican fruit fly, anastrepha ludens (loew), is known to be a major pest of citrus, doubt exists as to the status of citrus as a breeding host for a. serpentina. to evaluate the host status of commercial citrus for a. serp ...201121510184
Anastrepha ludens and Anastrepha serpentina (Diptera: Tephritidae) do not infest Psidium guajava (Myrtaceae), but Anastrepha obliqua occasionally shares this resource with Anastrepha striata in nature.This study examined whether economically important fruit fly species Anastrepha ludens (Loew), Anastrepha serpentina (Wiedemann), and Anastrepha obliqua (Macquart) (Diptera: Tephritidae) may opportunistically exploit guavas, Psidium guajava L. (Myrtaceae), growing near preferred natural hosts. We collected 3,459 kg of guavas and 895 kg of other known host species [sour orange, Citrus aurantium L.; grapefruit, Citrus paradisi Macfadyen; mango, Mangifera indica L.; white sapote, Casimiroa edulis L ...201121882684
grapefruit as a host for the west indian fruit fly (diptera: tephritidae).the most common hosts for the west indian fruit fly, anastrepha obliqua (macquart) (diptera: tephritidae) are fruit in the family anacardiaceae (mango [mangifera l.] and mombin [spondias l.] species). however, similar to many of the tropical fruit flies of major economic importance, this species attacks several other families of crop fruit, including annonaceae (cherimoya, annona cherimola mill.), myrtaceae (guava, psidium l.), oxalidaceae (carambola, averrhoa carambola l.), passifloraceae (gran ...201121404839
evaluation of the efficacy of the methyl bromide fumigation schedule against mexican fruit fly (diptera: tephritidae) in citrus fruit.methyl bromide fumigation is widely used as a phytosanitary treatment. mexican fruit fly, anastrepha ludens (loew) (diptera: tephritidae), is a quarantine pest of several fruit, including citrus (citrus spp.), exported from texas, mexico, and central america. recently, live larvae have been found with supposedly correctly fumigated citrus fruit. this research investigates the efficacy of the previously approved u.s. department of agriculture-animal and plant health inspection service treatment s ...201121404840
development of transgenic strains for the biological control of the mexican fruit fly, anastrepha ludens.the mexican fruit fly, anastrepha ludens, is a highly significant agricultural pest species that has been genetically transformed with a piggybac-based transposon vector system using independent vector and transposase helper plasmids. minimum estimated germ-line transformation frequencies were approximately 13-21% per fertile g(0) individual, similar to previously reported frequencies using single vector-helper plasmids. two vector constructs were tested with potential importance to transgenic s ...201120737195
a novel attractant for anastrepha ludens (diptera: tephritidae) from a concord grape product.an attractant for mexican fruit fly, anastrepha ludens (loew) (diptera: tephritidae), was developed from a commercial product called sabor uva containing processed concord grape juice. the principal volatile components of sabor uva aroma were identified and an aqueous mixture of 15 components that was gas chromatographically similar to sabor uva was prepared. this mixture was equivalent to sabor uva in attractiveness by using wind-tunnel bioassays. after deleting chemicals that did not contribut ...201121882683
light conditions affect sexual performance in a lekking tephritid fruit fly.sensory systems are very susceptible to early environment experience. mating success depends on the transmission of information from the signaller to the receiver, which means that sensory biases caused by developmental environment are likely to affect sexual selection. we investigated the impact of the developmental visual environment (light spectrum) on male copulation behaviour and female preference in the lekking tephritid anastrepha ludens. we reared flies in four different light spectrum c ...201121753054
efficacy of ground spray application of bait sprays with malathion or spinosad on mexican fruit fly (diptera: tephritidae) in texas citrus.an important component in the mexican fruit fly, anastrepha ludens (loew) (diptera: tephritidae), eradication program is bait spray application to knock down localized a. ludens infestations enhancing the sterile to wild fly ratio and increasing the effectiveness of the sterile insect release program. efficacy tests were conducted using spray equipment that applies ultralow application rates of malathion nu-lure or gf-120 spinosad by ground into citrus. trapit dome traps located in fields treate ...201121510192
resistance of mexican fruit fly to quarantine treatments of high-pressure processing combined with cold.mexican fruit fly anastrepha ludens loew (diptera: tephritidae) is one of the most important insects infesting fruits. although high pressure has been proposed as an alternative quarantine process for this pest, conditions applied to destroy eggs and larvae can also damage the fruits. the objective of this study was to assess the biological viability of a. ludens eggs treated by high-pressure processing at 0°c, establishing whether nondestroyed eggs and larvae preserved their ability to develop ...201121492022
residual control and lethal concentrations of gf-120 (spinosad) for anastrepha spp. (diptera: tephritidae).the objective of this study was to determine the relationship between residual time of gf-120 (spinosad) treatment and mortality in three species of anastrepha schiner. concentrations of 96, 72, 48, and 24 ppm were aged on mango leaves under field conditions for 0, 3, 7, 10, 14, 17, and 21 d after application. we found that anastrepha ludens, a. obliqua, and a. serpentina were highly sensitive to spinosad. the effects of spinosad were not reduced over the 4 d after the initial application, even ...201122299349
molecular technologies to improve the effectiveness of the sterile insect technique.the application of the sterile insect technique (sit) in area-wide integrated pest management (aw-ipm) programmes continues to increase. however, programme efficiency can still be considerably enhanced when certain components of the technology are improved, such as the development of improved strains for mass rearing and release. these include strains that (1) produce only male insects for sterilization and release and (2) carry easily identifiable markers to identify released sterile insects in ...201121258957
copulatory behaviour and the process of intromission in anastrepha ludens (diptera: tephritidae).complex genitalia occur in many arthropods and in some species extreme female morphologies lead to serious mechanical difficulties for males. tephritid flies offer examples of such complex genitalia. because of their economic importance and the extensive use of sterile male releases for tephritid control in texas and mexico, studies have been done on various aspects of their basic reproductive biology, but the process of intromission has received little attention. the distiphallus of the male of ...201121513199
comparison of in vitro heat and cold tolerances of the new invasive species bactrocera invadens (diptera: tephritidae) with three known tephritids.bactrocera invadens drew, tsuruta & white (diptera: tephritidae) is spreading throughout central africa attacking a variety of fruit; quarantines are placed on fruit from this region that are considered hosts. the only phytosanitary treatment that is commercially available is an ionizing irradiation treatment for all tephritidae at 150 gy. the development of other treatments, such as heat, cold, or fumigation, usually requires testing tens of thousands of insects at a dose that provides efficacy ...201121404834
a transgenic embryonic sexing system for anastrepha suspensa (diptera: tephritidae).the sterile insect technique (sit) is a highly successful biologically-based strategy to control pest insect populations that relies on the large-scale release of sterilized males to render females in the field non-reproductive. for medfly, a mutant-based sexing system is available as well as a transgenic system where a tetracycline-suppressible (tet-off) toxic molecule is female-specifically produced. however, the former classical genetic system took many years to refine, and the latter system ...201222858603
superparasitism in the fruit fly parasitoid diachasmimorpha longicaudata (hymenoptera: braconidae) and the implications for mass rearing and augmentative release.superparasitism, a strategy in which a female lays eggs in/on a previously parasitized host, was attributed in the past to the inability of females to discriminate between parasitized and non-parasitized hosts. however, superparasitism is now accepted as an adaptive strategy under specific conditions. in fruit fly parasitoids, superparasitism has mainly been studied as concerns the new association between diachasmimorpha longicaudata (ashmead) (hymenoptera: braconidae) and the mexican fruit fly ...201226466718
survey of heritable endosymbionts in southern mexico populations of the fruit fly species anastrepha striata and a. ludens.heritable endosymbiotic bacteria associated with insects are ubiquitous and taxonomically diverse. many of these endosymbionts influence the fitness of their hosts and/or manipulate their host reproduction. exploiting the effects of endosymbionts on hosts for pest control is a growing research area, but requires knowledge of endosymbionts associated with the target pest population. in this study, we used molecular methods to screen southern mexico populations of two species of tephritid fruit fl ...201222961037
host status of meyer and eureka lemons for anastrepha ludens.host status for mexican fruit fly (anastrepha ludens (loew)) was examined under laboratory conditions in cage infested eureka and meyer lemons. our approach was to allow females to oviposit on the two cultivars in separate laboratory cages with aluminum foil covering to restrict the areas where females had access to fruit surface. fruit of each cultivar were placed in covered trays for incubations and at approximately weekly intervals, fruit were removed, dissected, and live and dead eggs and la ...201222606804
prolongevity effects of a botanical with oregano and cranberry extracts in mexican fruit flies: examining interactions of diet restriction and age.botanicals rich with phytochemicals have numerous health benefits. dietary restriction (dr) extends lifespan in diverse species. we previously demonstrated that an oregano-cranberry (oc) mixture can promote longevity in the mexican fruit fly (mexfly, anastrepha ludens loew). however, little is known about the interaction between botanicals and dr, and the age-dependent effect of botanicals on lifespan and reproduction. here we investigated these issues by feeding mexflies a full or dr diet suppl ...201221455602
understanding long-term fruit fly (diptera: tephritidae) population dynamics: implications for areawide management.fruit flies (diptera: tephritidae) are devastating agricultural pests worldwide but studies on their long-term population dynamics are sparse. our aim was to determine the mechanisms driving long-term population dynamics as a prerequisite for ecologically based areawide pest management. the population density of three pestiferous anastrepha species [anastrepha ludens (loew), anastrepha obliqua (macquart), and anastrepha serpentina (wiedemann)] was determined in grapefruit (citrus x paradisi macf ...201222812118
the interplay among dietary fat, sugar, protein and açai (euterpe oleracea mart.) pulp in modulating lifespan and reproduction in a tephritid fruit fly.macronutrient balance is a critical contributor in modulating lifespan and health. consumption of diets rich in fruits and vegetables provides numerous health benefits. the interactions among macronutrients and botanicals and how they influence aging and health remain elusive. here we employed a nutritional geometry approach to investigate the interplay among dietary fat, sugar, protein and antioxidant- and polyphenolic-rich freeze-dried açai pulp in modulating lifespan and reproductive output i ...201222580089
phytosanitary cold treatment for oranges infested with bactrocera zonata (diptera: tephritidae).the peach fruit fly, bactrocera zonata (saunders), attacks a wide range of tree fruits in countries from egypt to vietnam and is occasionally trapped in the united states. phytosanitary treatments may be required to export fruit hosts of this insect from countries where it is endemic to countries where it is absent but could become established. this research describes comparative studies to determine if b. zonata could be phytosanitarily controlled by cold treatment schedules existing for cerati ...201324498731
tapachula-7, a new genetic sexing strain of the mexican fruit fly (diptera: tephritidae): sexual compatibility and competitiveness.a new genetic sexing strain of the mexican fruit fly, anastrepha ludens (loew), was evaluated in tests of sexual behavior to determine its possible application using the sterile insect technique. tests in field cages measuring time to sexual maturity, compatibility with wild flies, and competitiveness were compared between the genetic sexing strain, tapachula-7, and the mass-reared standard bisexual strain. the results indicated that the onset of sexual maturity was similar for both laboratory s ...201323786061
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