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reclassification of 'corynebacterium haemolyticum' (maclean, liebow & rosenberg) in the genus arcanobacterium gen.nov. as arcanobacterium haemolyticum nom.rev., comb.nov.'corynebacterium haemolyticum' (maclean, liebow & rosenberg) differs to such an extent from the type species of corynebacterium, c. diphtheriae (lehmann & neumann), that it should be removed from this genus. chemical and numerical phenetic data indicate that 'c. haemolyticum' is a distinct taxon worthy of generic status. a new genus, arcanobacterium, is described for the species a. haemolyticum (maclean, liebow & rosenberg) nov.rev., comb.nov. the genus is tentatively placed within the 'corynefo ...19827119737
[septicemia caused by arcanobacterium haemolyticum (corynebacterium haemolyticum) and streptococcus milleri]. 19863751438
comparative studies on actinomyces pyogenes and arcanobacterium haemolyticum.actinomyces pyogenes and arcanobacterium haemolyticum were further characterized. a. haemolyticum, in contrast to a. pyogenes, gave synergistic hemolytic reactions with streptococci of serological group b and inhibited staphylococcal beta-hemolysis. a. pyogenes and a. haemolyticum had a number of common, as well as distinctly different enzymatic properties, useful for their characterization. extracts from a. pyogenes reacted with antiserum against streptococci of serological group g in contrast ...19883129637
serological recognition of actinomyces pyogenes and arcanobacterium haemolyticum in serum soft agar. 19883381600
paravertebral abscess due to nondiphtheria coryneform bacteria as a complication of ingrown toenails.a patient developed a paravertebral abscess due to nondiphtheria coryneform bacteria following infected ingrown toenails. both the causative role of this uncommon pathogen and the unusual origin were established by cultures of tissue from the toes, blood and an aspirate of the paravertebral abscess. the microorganism was tentatively identified as an atypical variant of arcanobacterium haemolyticum.19892493428
[pharyngitis caused by arcanobacterium haemolyticum]. 19892729792
penicillin tolerance in arcanobacterium haemolyticum.it was previously found that arcanobacterium haemolyticum, which can cause tonsillitis with exanthema, is not eradicated from the pharynx by administration of phenoxymethylpenicillin, despite minimum inhibitory concentration values of 0.015-1.0 micrograms/ml. therefore, recent clinical isolates were studied for penicillin tolerance by using a disk diffusion screening test and a pour plate assay. macrobroth dilution minimum inhibitory and bactericidal concentrations and antibiotic kill kinetics w ...19902105360
posttraumatic ankle joint infection with arcanobacterium haemolyticum: a case report. 19902117630
selective medium for isolating arcanobacterium haemolyticum. 19902380412
arcanobacterium haemolyticum brain abscess in a child (a case report). 19911752643
arcanobacterium haemolyticum: biology of the organism and diseases in man. 19911766710
selective medium for isolation of arcanobacterium haemolyticum and streptococcus pyogenes.sheep blood agar using tryptic soy agar base and containing 3.5% sodium chloride was found to facilitate isolation of arcanobacterium haemolyticum and improve isolation of voges-proskauer negative group a (streptococcus pyogenes) and group c and g beta-haemolytic streptococci. commensal oropharyngeal flora such as viridans streptococci, neisseria species, non-haemolytic streptococci and beta-haemolytic streptococci not belonging to groups a, b, c or g were significantly inhibited.19911874249
cellular fatty acid composition as an adjunct to the identification of asporogenous, aerobic gram-positive rods.cellular fatty acid (cfa) compositions of 561 asporogenous, aerobic gram-positive rods were analyzed by gas-liquid chromatography as an adjunct to their identification when grown on blood agar at 35 degrees c. the organisms could be divided into two groups. in the first group (branched-chain type), which included coryneform cdc groups a-3, a-4, and a-5; some strains of b-1 and b-3; "corynebacterium aquaticum"; brevibacterium liquefaciens; rothia dentocariosa; and listeria spp., the rods had siza ...19911899679
radiological case of the month. cavitary pneumonia due to arcanobacterium hemolytic. 19911994689
grouping of some clinically relevant gram-positive rods by automated fatty acid analysis. diagnostic implications.one hundred and ninety-four strains of aerobically growing gram-positive rods of the genera corynebacterium, actinomyces, arcanobacterium, erysipelothrix, listeria, oerskovia, nocardia, rhodococcus, and of unnamed center for disease control (cdc) groups were checked for cellular fatty acid profiles with the microbial identification system (microbial id, newark, del., usa). in order to obtain unified data usable for the clinical laboratory, 24 or 48 h sheep blood agar cultures were used. it was t ...19912001281
arcanobacterium hemolyticum infection: confused with scarlet fever and diphtheria.arcanobacterium hemolyticum infections are a common cause of pharyngitis and rash in the 10- to 30-year-old age group. despite its prevalence, many emergency and primary care physicians may not be aware of the pathogenic potential of this organism. we present a case that illustrates the distinctive clinical spectrum of a. hemolyticum infections that may be confused with drug allergy, group a streptococcal scarlet fever, diphtheria, and even toxic shock syndrome. recognition of this syndrome will ...19912045646
arcanobacterium haemolyticum sepsis and epstein-barr virus infection. 19921321407
isolation of arcanobacterium haemolyticum from patients with pharyngitis in belgium.during a 40 months period, 49 arcanobacterium haemolyticum strains were isolated from 12,000 throat swabs of patients with pharyngitis originating from 5 belgian provinces. all strains were recovered on sheep blood columbia agar plates, supplemented by nalidixic acid and colistin. in two thirds of the cases, pharyngitis was associated with a rash. half of the cases occurred in the age group between 10 and 20 years and isolates were twice more frequent in females than in males. streptococcus pyog ...19921334318
arcanobacterium haemolyticum in children with presumed streptococcal pharyngotonsillitis or scarlet fever.arcanobacterium haemolyticum was cultured from pharyngeal specimens obtained from 12 of 129 children with pharyngotonsillitis, some of whom had a scarlatiniform rash. this organism should be considered to be a cause of infections that are clinically similar to those caused by beta-hemolytic streptococci.19921432423
evaluation of the rapid ana ii and api zym systems for identification of actinomyces species from clinical specimens.classification and identification of fermentative actinomycetes are labor-intensive and problematic. in this study, we evaluated the applicability and reliability of the rapid ana ii system (innovative diagnostic systems, inc., atlanta, ga.) and the discriminatory value of the api zym system (societes analytab products inc., la balme les grottes, france) in the identification of actinomyces-like bacteria by using conventional methods as a reference. eighty-five strains, including 71 isolates fro ...19921452693
acute peritonsillar abscess caused by arcanobacterium haemolyticum.a patient is reported with a peritonsillar abscess yielding arcanobacterium haemolyticum. this appears to be only the fifth such case described in the medical literature and the first from europe. the organism has been reported as an occasional cause of tonsillopharyngitis with rash, resembling infection with streptococcus pyogenes but often unresponsive to penicillin therapy. a. haemolyticum easily passes unrecognized in bacteriological cultures as a result of its slow growth, coryneform appear ...19921479262
evaluation of the api coryne test system for identification of actinomyces pyogenes.the present study was designed to evaluate the accuracy of the api coryne test system for identification of actinomyces pyogenes. the test system correctly identified 36 of 42 a. pyogenes and 4 of 5 comparatively studied arcanobacterium haemolyticum-cultures. the biochemical profiles of the remaining 6 a. pyogenes- and 1 a. haemolyticum-cultures were not included in the analytical profile index. none of the cultures were misidentified. according to the api database (atb plus v 1.5.4.) the uniden ...19921519407
arcanobacterium haemolyticum wound infection. 19921569313
differentiation of corynebacterium spp., listeria spp., and related organisms by using fluorogenic substrates.a total of 228 strains of arcanobacterium haemolyticum, corynebacterium spp., erysipelothix rhusiopathiae, and listeria spp. were investigated for their abilities to hydrolyze 60 different fluorogenic 4-methylumbelliferyl-linked and beta-naphthylamide-linked substrates within 6 and 24 h of incubation. the hydrolysis of a group of 16 fluorogenic substrates, and in particular, the glycosidase tests, in most cases showed high separation values at the genus level. when used in combination with other ...19921583102
[actinomyces pyogenes: conventional and api system bacteriologic study of 103 strains isolated from ruminants].one hundred and three strains of actinomyces pyogenes isolated from ruminants were examined for morphological, cultural and biochemical properties by standard tests and by the api 50 ch and api coryne methods. no biotype could be demonstrated, but a few atypical non- or practically non-proteolytic strains were detected which should be differentiated from arcanobacterium haemolyticum. criteria for laboratory identification of a pyogenes were established: gram stain; culture on blood agar; deep ag ...19921610079
human infections with actinomyces pyogenes (corynebacterium pyogenes).actinomyces pyogenes (corynebacterium pyogenes), a well-known pathogen in many animals, was isolated from 11 danish patients since 1968. bacteriologic characteristics and clinical pictures of the patients are described. ability to hydrolyze gelatine, to produce beta-glucuronidase, to reach with antisera against group-g streptococci, and to produce acid from xylose differentiates a. pyogenes from arcanobacterium haemolyticum, with which it has at times been confused. actinomyces pyogenes is an es ...19921611850
evaluation of the rapid coryne identification system for corynebacterium species and other coryneforms.the rapid coryne system for identification of aerobic, nonsporeforming, gram-positive rods was evaluated according to the manufacturer's instructions with 177 organisms. after inoculation with a heavy suspension of growth, strips containing 20 cupules were incubated for 24 h, reagents were added, and the results of 21 biochemical reactions were recorded as numerical profiles. the strains consisted of pathogenic species of the genus corynebacterium, primarily c. diphtheriae (n = 29), opportunisti ...19921629322
isolation of arcanobacterium haemolyticum from an infected foot wound. 19938102967
characteristics of cdc group 1 and group 1-like coryneform bacteria isolated from clinical specimens.fifteen strains of cdc group 1 coryneform and biochemically similar bacteria were isolated from clinical specimens. of the 15 strains isolated, 11 were derived from abscesses and purulent lesions, mostly from the upper part of the body, and 3 were grown from blood cultures. nine strains were associated with mixed anaerobic but no other aerobic flora. seven strains exhibited the classical biochemical profile of cdc coryneform group 1; however, eight strains were unable to reduce nitrate and were ...19938263175
effects of media, atmosphere, and incubation time on colonial morphology of arcanobacterium haemolyticum.arcanobacterium haemolyticum causes pharyngitis as well as skin and other wound infections. although it is a beta-hemolytic organism, the hemolysis is less well defined than that of beta-hemolytic streptococci and may be overlooked in cultures with heavy growth of commensal throat flora. to determine whether routine throat culture conditions are sufficient to produce recognizable colonies of a. haemolyticum, the morphology of six distinct strains was studied after various combinations of incubat ...19938308114
arcanobacterium haemolyticum phospholipase d is genetically and functionally similar to corynebacterium pseudotuberculosis phospholipase d.arcanobacterium haemolyticum, a pathogen of the human upper respiratory tract and other systems, has been reported to produce soluble toxins, including a phospholipase d (pld). we confirmed production of pld by this organism and cloned and sequenced pld. arcanobacterial pld (pld-a) was found to be a protein of approximately 31.5 kda with a pi of approximately 9.4. cosmid cloning, followed by subcloning into phagemid pbluescriptiisk+, yielded escherichia coli(pah140), a recombinant with a gene pr ...19938406819
[arcanobacterium haemolyticum angina]. 19938502651
antimicrobial susceptibility of arcanobacterium haemolyticum.the susceptibilities of 138 clinical isolates of arcanobacterium haemolyticum to 11 antimicrobial agents were tested. all strains were susceptible to phenoxymethylpenicillin, cephalosporins, erythromycin, azithromycin, clindamycin, vancomycin, doxycycline, and ciprofloxacin but were resistant to trimethoprim-sulfamethoxazole.19948141569
[bacterial epidemiology of pharyngitis in pediatric private practice].while viruses are usually the causal agents of common sore throat in children, bacterial infections cannot be distinguished solely on the basis of clinical presentation. thus most physicians in france prefer to prescribe antibiotics in order to prevent rheumatismal complications of group a streptococcal infections. we updated current epidemiological data on bacterial pharyngitis in paediatric out-patient clinics.19947831263
alpha-mannosidase in arcanobacterium haemolyticum. 19947852597
biotypes of arcanobacterium haemolyticum.colony morphology, beta hemolysis on horse blood agar, beta-glucuronidase activity, and ability to ferment sucrose and/or trehalose defined two biotypes of arcanobacterium haemolyticum. one, the smooth type, grew as smooth, beta-hemolytic colonies and was beta-glucuronidase negative but often fermented sucrose and/or trehalose, while the other, the rough type, grew as rough colonies and was nonhemolytic, beta-glucuronidase positive, and negative for sucrose and trehalose fermentation. about 75% ...19947929753
arcanobacterium haemolyticum and streptococcal pharyngitis.arcanobacterium haemolyticum was recovered from 0.5% of throat cultures of 3,922 patients seeking medical attention because of sore throat. most of the patients positive for a. haemolyticum were 15-25 years old, and had fever (80%), lymphadenopathy (67%), pharyngeal exudate (69%) or skin rash (23%). in this age group, 2% of the throat cultures proved positive for a. haemolyticum. all a. haemolyticum strains were susceptible to penicillin, erythromycin, cephalexin and clindamycin, but resistant t ...19947939427
evaluation of a commercial kit in the identification of arcanobacterium haemolyticum and actinomyces pyogenes.listeria-zym is a commercial kit designed to identify listeria spp. within 4 h. its ability to identify two clinically important aerobic catalase-negative, gram-positive rods, arcanobacterium haemolyticum and actinomyces pyogenes, was evaluated. xylose fermentation and alpha-mannosidase tests differentiated arcanobacterium haemolyticum (n = 49) from actinomyces pyogenes (n = 24) strains.19947957274
two cases of soft-tissue infection caused by arcanobacterium haemolyticum. 19948075288
alpha-mannosidase: a rapid test for identification of arcanobacterium haemolyticum.a 4-h alpha-mannosidase test for identification of arcanobacterium haemolyticum strains (n = 139) and differentiation of a. haemolyticum from actinomyces pyogenes strains (n = 30) and other gram-positive rods was evaluated. practically all a. haemolyticum strains (138 of 139) and the listeria monocytogenes type strain were alpha-mannosidase positive, while all a. pyogenes strains and corynebacterium (n = .25) strains as well as the rhodococcus equi and erysipelothrix rhusiopathiae type strains w ...19947710479
toxic phospholipases d of corynebacterium pseudotuberculosis, c. ulcerans and arcanobacterium haemolyticum: cloning and sequence homology.the genes encoding toxic phospholipases d (pld) from corynebacterium pseudotuberculosis (cp)biovar equi and c. ulcerans (cu) have been cloned and sequenced. the deduced proteins are 307 amino acids (aa) in length and include a putative signal sequences of 26-aa. a molecular mass of 31.2 and 31.0 kda and pi values of 8.84 and 6.73 are predicted for the secreted (mature) proteins from cp and cu, respectively. comparison of the deduced primary structure of the two proteins to those of the pld produ ...19957737503
arcanobacterium haemolyticum and streptococcal pharyngitis in army conscripts.arcanobacterium haemolyticum was found in 1.4% and beta-haemolytic streptococci in 23% of throat cultures from army conscripts with sore throat (n = 498). 38% of the beta-haemolytic streptococci were of group a. patients culture-positive for a. haemolyticum or beta-haemolytic streptococci had pharyngeal exudate, cervical lymphadenopathy and ear ache significantly more often--but cough less often--than culture-negative patients. the pharyngeal colonization rate of healthy conscripts (n = 232) by ...19957784807
additional tests to differentiate arcanobacterium haemolyticum and actinomyces pyogenes.a commercially available biochemical test panel, commercially available diagnostic tablets and gas-liquid chromatography (glc) of cellular fatty acids were used to find out whether arcanobacterium haemolyticum and actinomyces pyogenes could be further differentiated from each other. xylitol and alpha-methyl-d-glucoside fermentation, voges-proskauer reaction and tributyrate hydrolysis were found to be useful additional tests which differentiated arc. haemolyticum and a. pyogenes. glc analysis rev ...19957549154
incidence and pathogenicity of arcanobacterium haemolyticum during a 2-year study in ottawa.arcanobacterium haemolyticum has been described as a rare cause of systemic invasive disease and is occasionally isolated from throat swabs. we describe a 2-year study of the incidence and clinical features of a. haemolyticus infection in a pediatric and adolescent population. a total of 11,620 throat swabs were examined for a. haemolyticum with use of a locally developed selective medium. controls (2,241) were healthy students who were recruited from a separate study. a. haemolyticum was isolat ...19957578727
orbital cellulitis, subperiosteal abscess, sinusitis, and septicemia caused b arcanobacterium haemolyticum.purpose/methods: a 16-year-old boy had orbital cellulitis, subperiosteal abscess, sinusitis, and septicemia. arcanobacterium haemolyticum was identified as the causative organism. results/conclusions: this organism is a cause of orbital cellulitis and may require aggresive therapy in order to achieve a therapeutic response.19957639318
are penicillin treatment failures in arcanobacterium haemolyticum pharyngotonsillitis caused by intracellularly residing bacteria?arcanobacterium haemolyticum is an infrequent agent of pharyngotonsillitis in children and young adults. despite the fact that a. haemolyticum is fully sensitive to penicillin in vitro, penicillin treatment failures are frequent. the ability of a. haemolyticum to invade hep-2 cells and survive intracellulary was investigated. all 12 strains tested, of which 10 were isolated from patients with pharyngotonsillitis, and 2 were reference strains, were internalized by the hep-2 cells. four strains te ...19957660075
group a streptococcal tonsillopharyngitis: cost-effective diagnosis and treatment.most patients who seek medical attention for sore throat are concerned about streptococcal tonsillopharyngitis, but fewer than 10% of adults and 30% of children actually have a streptococcal infection. group a beta-hemolytic streptococci (gas) are most often responsible for bacterial tonsillopharyngitis, although neisseria gonorrhea, arcanobacterium haemolyticum (formerly corynebacterium haemolyticum), chlamydia pneumoniae (twar agent), and mycoplasma pneumoniae have also been suggested as possi ...19957864482
nosocomial bacterial and fungal meningitis in cancer patients.five cases of nosocomial meningitis are described that occurred within 5 years in a national cancer center in neutropenic cancer patients after cytotoxic chemotherapy: one caused by the yeast aureobasidium mansoni and four caused by bacteria (two by enterococcus faecalis and one by salmonella enteritis and arcanobacterium haemolyticus. despite the severe symptoms, all cases were cured with appropriate antimicrobial therapy.19958564349
vana genes in vancomycin-resistant clinical isolates of oerskovia turbata and arcanobacterium (corynebacterium) haemolyticum.we report the cloning and sequencing of vana genes present in the high-level vancomycin- and teicoplanin-resistant clinical isolates oerskovia turbata 892 and arcanobacterium (corynebacterium) haemolyticum 872. the presence of vana was detected by southern blotting and pcr and confirmed by dna sequencing. vana-like sequences were encoded on plasmids of 15 and 20 kb respectively. the a. haemolyticum 872 dna sequence was identical to the published vana sequence of vancomycin-resistant enterococcus ...19958591934
isolation of arcanobacterium haemolyticum from throat culture samples in the czech republic. 19958655218
endocarditis caused by arcanobacterium haemolyticum.a 33-year-old, active intravenous drug-abusing male infected with the human immunodeficiency virus developed endocarditis due to arcanobacterium haemolyticum. empirical treatment with ampicillin plus gentamicin failed to achieve a marked clinical improvement. when the results of antibiotic susceptibility were available (ampicillin mic < or = 0.06 mg/l; ampicillin mbc 2 mg/l; mbc:mic ratio > 32) therapy was changed to vancomycin plus gentamicin. during the following days progressive clinical and ...19958681985
report of arcanobacterium haemolyticum meningitis in a cancer patient. 19968708750
an identification scheme for rapidly and aerobically growing gram-positive rods.an identification scheme for aerobically growing gram-positive rods (genera actinomyces, arcanobacterium, aureobacterium, bacillus, brevibacterium, cellulomonas, corynebacterium, dermabacter, erysipelothrix, gardnerella, lactobacillus, listeria, microbacterium, oerskovia, propionibacterium, rhodococcus, rothia, turicella, as well as unnamed cdc groups, clostridium tertium, and mycobacterium fortuitum/chelonae) is presented. it is derived from the hollis-weaver scheme and uses catalase, oxidative ...19968837385
microbial colonizers in leprosy skin ulcers and intensity of inflammation.the microflora of 55 patients with leprosy skin ulcers was studied and related to a weighted inflammatory score (is). the control group consisted of 18 ulcers with different underlying pathology. leprosy ulcers were characterized by the exclusive presence of two types of branching gram-positive rods; a particular interesting proposal is that mycobacterium leprae share common antigens with these unusual "leprosy ulcer associated" organisms and group g beta-hemolytic streptococci. in the leprosy g ...19968862261
arcanobacterium haemolyticum pharyngitis and exanthem. three case reports and literature review.arcanobacterium haemolyticum is a bacterial pharyngeal pathogen that infects adolescents and young adults, frequently causing an exanthem that may mimic a viral exanthem, toxic erythema, or drug eruption. to our knowledge, the cutaneous manifestations of a haemolyticum infection have not previously been reported in the dermatologic literature.19968546485
[pharyngitis produced by arcanobacterium haemolyticum].arcanobacterium haemolyticum (a.h.) was recovered from 0.43% of throat cultures of 3715 patients with sore throat, scarlatina and various skin rash. in a 57.90% patients a.h. was the only bacterial pathogen isolated, while in the remainder beta-haemolytic streptococci, haemophilus and s. aureus were also detected. beta-haemolytic streptococci were much more frequent than a.h. in the throat cultures (26.83%). all a.h. strains were of the smooth type based on colony morphology and the ability to f ...19969116401
[the isolation of arcanobacterium haemolyticum from the pharyngeal exudate of children].a. haemolyticum is involved in acute pharingotonsillitis in present, reaching a maximal incidence between 10 and 30 years of age. the authors examined 7402 throats swabs obtained from children between march 1995 and february 1996. the swabs were plated on a selective culture medium: 5% sheep blood agar added with 3.5% nacl; the plates were incubated for 48 h in aerobic conditions. the colonies of a. haemolyticum have been suspected on the basis of a) the growing time interval (48 h); reduced siz ...19969116402
arcanobacterium haemolyticum invasive infections, including meningitis in cancer patients. 19979120339
antibody response to arcanobacterium haemolyticum infection in humans.arcanobacterium haemolyticum causes pharyngitis, exanthema, and other infections. the evidence of the pathogenicity of a. haemolyticum depends on clinical descriptions of culture-positive patients and a comparison of carrier rates of patients with pharyngitis and healthy, matched controls. in this investigation, the antibody response of the host was studied for the first time, using sds-page and western blot analyses. paired acute and convalescent sera showed development of antibodies to a. haem ...19979180197
rhodococcus equi and arcanobacterium haemolyticum: two "coryneform" bacteria increasingly recognized as agents of human infection.rhodococcus equi and arcanobacterium haemolyticum, formerly classified in the genus corynebacterium, are members of the loosely defined taxon "coryneform" bacteria. although they are the etiologic agents of distinct human infections, both organisms are frequently overlooked, which results in missed or delayed diagnoses. r. equi, long known as an important pathogen of immature horses, has become in the past three decades an opportunistic pathogen of severely immunosuppressed humans. most cases ar ...19979204295
characterization of some actinomyces-like isolates from human clinical specimens: reclassification of actinomyces suis (soltys and spratling) as actinobaculum suis comb. nov. and description of actinobaculum schaalii sp. nov.five strains of a hitherto unknown actinomyces-like bacterium were isolated from human clinical sources, including blood cultures. biochemical and chemotaxonomic characterization indicated that the strains were distinct from previously described actinomyces and arcanobacterium species. a comparative 16s rrna gene sequence analysis demonstrated that the undescribed strains constitute a new subline within the actinomyces-arcanobacterium species complex. the closest known relative of the isolates w ...19979226926
the arcanobacterium (actinomyces) pyogenes hemolysin, pyolysin, is a novel member of the thiol-activated cytolysin family.arcanobacterium (actinomyces) pyogenes, an animal pathogen, produces a hemolytic exotoxin, pyolysin (plo). the gene encoding plo was cloned, and sequence analysis revealed an open reading frame of 1,605 bp encoding a protein of 57.9 kda. plo has 30 to 40% identity with the thiol-activated cytolysins (tacys) of a number of gram-positive bacteria. the activity of plo was found to be very similar to those of other tacys, except that it was not thiol activated. the highly conserved tacy undecapeptid ...19979324258
electroporation-mediated transformation of arcanobacterium (actinomyces) pyogenes.plasmids derived from png2 or rsf1010 were introduced into strains of arcanobacterium (actinomyces) pyogenes by electroporation. electroporation conditions were varied systematically to give a maximum electroporation frequency of 3.7 x 10(5) cfu/microgram dna at 1.5 kv/cm and 246 omega, resulting in a time constant of approximately 10 ms. the a. pyogenes transformants expressed plasmid-encoded resistance to chloramphenicol, erythromycin, kanamycin, and streptomycin. the source of incoming dna af ...19979339471
isolation, in vitro antimicrobial susceptibility and penicillin tolerance of arcanobacterium haemolyticum in a turkish university hospital.arcanobacterium haemolyticum (ah) was isolated from 5 (0.3%) out of 1531 throat cultures of patients with presumed pharyngotonsillitis. the age of the patients who had a positive culture for ah varied between 6 and 22. the isolation rate of beta-haemolytic streptococci (bhs) was 7.4%, 72.6% of which belonged to group a, followed by groups g, c and b. none of the throat samples yielded simultaneous growth of ah and bhs. antimicrobial susceptibility of ah isolates to phenoxymethylpenicillin, cepha ...19979440197
clinical microbiology of coryneform bacteria.coryneform bacteria are aerobically growing, asporogenous, non-partially-acid-fast, gram-positive rods of irregular morphology. within the last few years, there has been a massive increase in the number of publications related to all aspects of their clinical microbiology. clinical microbiologists are often confronted with making identifications within this heterogeneous group as well as with considerations of the clinical significance of such isolates. this review provides comprehensive informa ...19978993861
phylogenetic analysis of the genus actinomyces based on 16s rrna gene sequences: description of arcanobacterium phocae sp. nov., arcanobacterium bernardiae comb. nov., and arcanobacterium pyogenes comb. nov.a systematic phylogenetic analysis of the genus actinomyces was performed. the 16s rrna gene sequences of 13 actinomyces species, an unnamed actinomyces strain (atcc 49338), and an actinomyces-like isolate from sea mammals were determined. comparative sequence analysis with closely related taxa revealed phylogenetic diversity and internal structure within the genus actinomyces. in addition, some members of other genera (viz., the genera arcanobacterium, mobiluncus, and rothia) were shown to be p ...19978995800
evaluation of the rapid cb plus system for identification of corynebacterium species and other gram-positive rods.due to the difficulty of identifying corynebacterium spp. with standard methods, we compared them with the rapid cb plus system (remel, lenexa, kans. [formerly innovative diagnostic systems, norcross, ga.]), which consists of 4 carbohydrate and 14 preformed enzyme tests, for the identification of 98 clinical isolates of corynebacterium sp., other coryneforms, listeria monocytogenes, and 17 atcc strains. forty (95%) of 42 strains of corynebacterium spp. were accurately identified to the species l ...19989466773
the arcanobacterium (actinomyces) pyogenes plasmid pap1 is a member of the pij101/pjv1 family of rolling circle replication plasmids.the 2.4-kb plasmid pap1 from arcanobacterium (actinomyces) pyogenes had sequence similarity within the putative replication protein and double-stranded origin with the pij101/pjv1 family of plasmids. pjgs84, a derivative of pap1 containing a kanamycin resistance gene, was able to replicate in escherichia coli and corynebacterium pseudotuberculosis, as well as in a. pyogenes. detection of single-stranded dna intermediates of pjgs84 replication suggested that this plasmid replicates by the rolling ...19989620977
septic arthritis due to arcanobacterium bernardiae in an immunocompromised patient. 19989675479
characterization of actinomyces turicensis and actinomyces radingae strains from human clinical samples.whole-organism protein electrophoresis was used to compare and group unidentified coryneform bacteria resembling gardnerella vaginalis and various actinomyces and arcanobacterium species. the obtained clusters of strains were further characterized by whole-cell fatty acid analysis and a variety of biochemical tests. species-specific oligonucleotide probes based on 16s rrna gene sequences were designed. the results demonstrate that the majority of the isolates belonged to actinomyces turicensis; ...19989731291
actinomyces pyogenes septic arthritis in a diabetic farmer.we report a case of septic arthritis and osteomyelitis of the left ankle due to actinomyces pyogenes in a diabetic farmer. few confirmed human cases of a. pyogenes infection have been reported, partly because of inadequate identification of this bacterium. bacteriological characteristics of the organism, which resembles arcanobacterium haemolyticum, are described with a review of previous case reports.19989733386
[bacteriological and clinical aspects of corynebacterium].the microbiologists use the term corynebacteria to describe aerobically growing, asporogenous, irregularly sharped gram-positive rods. they comprise strictly aerobic bacteria isolated from environment as well as preferentially anaerobic bacteria found in clinical specimens. a large part of these bacteria is considered as commensal of skin and mucous membranes. this group of organisms has recently been subjected to considerable taxonomic revisions, which have resulted in the proposal of several n ...19989754261
urinary tract infection due to arcanobacterium bernardiae in a patient with a urinary tract diversion. 19989758280
systemic and deep-seated infections caused by arcanobacterium haemolyticum.arcanobacterium haemolyticum has been implicated mainly in non-streptococcal pharyngitis and wound infections. rarely, it has been reported to cause systemic infection, often in combination with other pathogens. two cases of systemic and deep-seated infections caused by arcanobacterium haemolyticum are reported, and the literature is reviewed. sixteen cases of bacteremia and seven cases of non-bacteremic deep-seated have been published previously. eight of the bacteremic and two of the non-bacte ...19989796659
ulcerative tonsillopharyngitis due to arcanobacterium haemolyticum--a case report. 19989808913
phospholipase d-neutralization in serodiagnosis of arcanobacterium haemolyticum and corynebacterium pseudotuberculosis infections.phospholipase d (pld) neutralization was used to examine sera of humans (n = 40) with a spontaneous infection by arcanobacterium haemolyticum, sheep and goats (n = 76 and 79 respectively) with a spontaneous infection by corynebacterium pseudotuberculosis, mice (n = 26) experimentally immunized with pld from a. haemolyticum (pld-a) and mice (n = 28) experimentally immunized with pld from c. pseudotuberculosis (pld-c). pld-a and pld-c were also used as neutralizing antigens. a positive result of n ...19989987184
an arcanobacterium (actinomyces) pyogenes mutant deficient in production of the pore-forming cytolysin pyolysin has reduced virulence.pyolysin (plo), the hemolytic exotoxin expressed by arcanobacterium (actinomyces) pyogenes, is a member of the thiol-activated cytolysin family of bacterial toxins. insertional inactivation of the plo gene results in loss of expression of plo with a concomitant loss in hemolytic activity. the plo mutant, plo-1, has an approximately 1. 8-log10 reduction in the 50% infectious dose compared to that for wild-type a. pyogenes in a mouse intraperitoneal infection model. studies involving cochallenge o ...199910085010
arcanobacterium haemolyticum and mycoplasma pneumoniae co-infection.systemic infection caused by arcanobacterium haemolyticum is uncommon. we report a case of empyema and bacteraemia caused by this organism concomitant with mycoplasma pneumoniae infection.199910090506
bacteria associated with clinical mastitis in dairy heifers.a 1-yr field investigation of clinical mastitis in heifers was carried out in 24 veterinary districts in norway. quarter lacteal secretions from cases that occurred prepartum or within 14 d postpartum were examined bacteriologically. the study included 1040 heifers with clinical mastitis, and the total number of quarters that were clinically affected was 1361. the organisms that were most frequently isolated from samples from these quarters were staphylococcus aureus (44.3%), streptococcus dysga ...199910212457
expression of inducible nitric oxide synthase in spontaneous bovine bronchopneumonia.the expression of inducible nitric oxide synthase (inos), major histocompatibility class ii molecules (mhc-ii), cd68, and the calcium-binding proteins s100a8 and s100a9 (also called mrp8 and mrp14, respectively) was assessed in lung tissues from cattle that succumbed to pneumonia. expression patterns of these markers were related to the types of lung lesion. inos expression was only observed in lungs infected with arcanobacterium pyogenes or pasteurella haemolytica but not in lungs from cattle w ...199910490207
three cases of arcanobacterium haemolyticum associated with abscess formation and cellulitis.arcanobacterium haemolyticum has been described as an unusual pathogen causing pharyngotonsillitis and systemic disease in patients with predisposing conditions. a case of soft tissue abscess with no apparent portal of entry is reported in a healthy 31-year-old man who presented with a breast tumor. a second case of abscess formation in a 50-year-old patient with complicated wound healing is presented. in addition, a case of arcanobacterium haemolyticum cellulitis in a 25-year-old female is repo ...199910614955
antimicrobial susceptibilities and biotypes of arcanobacterium haemolyticum blood isolates.isolates obtained from the blood of ten patients with arcanobacterium haemolyticum septicaemia were biotyped as smooth or rough using morphological and biochemical criteria, and their susceptibilities to 18 antibacterial agents were determined. nine of the clinical cases included here have not been reported previously and are discussed in brief. one of the strains was highly resistant to macrolides and clindamycin. with one exception, the strains belonged to the smooth biotype. the data presente ...199910691208
relationships among seminal culture, seminal white blood cells, and the percentage of primary sperm abnormalities in bulls evaluated prior to the breeding season.semen samples from 100 beef breed bulls were evaluated for sperm morphology (phased contrast microscopy), seminal white blood cells, and the presence of potential reproductive pathogens. eligibility required visualization of the glans penis throughout semen collection. based on clinical spermiograms, bulls were grouped into normal, marginal, or unsatisfactory morphology classifications. the 3 experimental groups were similar in age and scrotal circumference and differed significantly in the perc ...199910729037
teat lesions and their relationship to intramammary infections on small-scale dairy farms in kiambu district in kenya.mammary gland quarters of 139 lactating dairy cows from small-scale dairy herds were examined visually and by palpation for teat lesions and by california mastitis test (cmt) and bacterial culture for subclinical mastitis. teat lesions were observed in 97 teats. these included teat chaps (39.2%), teat papillomas (23.7%), teat erosions (22.7%), teat fistulae (5.1%), inverted teats (5.1%) and blocked teats (4.2%). according to the cmt, the prevalence of subclinical mastitis was 33.4% in all the ma ...199910855839
arcanobacterium pyogenes as a cause of fatal pleuropneumonia after capture and transport of white-tailed deer (odocoileus virginianus). 199912968764
[do you recognize the pharyngitis and dermatitis caused by arcanobacterium haemolyticum?]. 200011989007
upper respiratory tract infections in adolescents.while upper respiratory tract infections (urtis) cause much infectious morbidity in infants and young children, adolescents are not immune to infections. adolescents experience two to four episodes of viral nasopharyngitis annually. in addition to group a streptococcus (gas), pharyngitis may occur with other streptococci, arcanobacterium haemolyticum, epstein-barr virus, neisseria gonorrhoeae, and other pathogens. uvulits, typically in association with gas, occurs occasionally. peritonsillar abs ...200010916122
actinomyces canis sp. nov., isolated from dogs.three strains of a previously undescribed catalase-positive actinomyces-like bacterium were isolated from dogs. biochemical testing and page analysis of whole-cell proteins indicated that the strains were phenotypically highly related to each other but different from previously described actinomyces and arcanobacterium species. sequencing of 16s rrna showed that the unknown bacterium represents a new subline within a cluster of species which includes actinomyces hyovaginalis, actinomyces georgia ...200010939662
characterization of actinomyces isolates from samples from the human urogenital tract: description of actinomyces urogenitalis sp. nov.three strains of a previously undescribed actinomyces-like bacterium were isolated from human clinical sources (urine, urethra and vaginal secretion). biochemical testing and page analysis of whole-cell proteins indicated that the strains were phenotypically homogeneous and distinct from previously described actinomyces and arcanobacterium species. comparative 16s rrna gene sequencing studies showed the bacterium to be a hitherto unknown subline within a group of actinomyces species which includ ...200010939672
characterization of actinomyces isolates from infected root canals of teeth: description of actinomyces radicidentis sp. nov.two strains of a previously undescribed actinomyces-like bacterium were recovered in pure culture from infected root canals of teeth. analysis by biochemical testing and polyacrylamide gel electrophoresis of whole-cell proteins indicated that the strains closely resembled each other phenotypically but were distinct from previously described actinomyces and arcanobacterium species. comparative 16s rrna gene-sequencing studies showed the bacterium to be a hitherto unknown subline within a group of ...200010970390
[a diagnostic medium for arcanobacterium haemolyticum and other bacterial species reacting with hemolytic synergism to the equi-factor of rhodococcus equi].colonies of arcanobacterium haemolyticum on common blood agar can be easily overlooked. therefore a diagnostic medium was developed, on which a. haemolyticum colonies produce a conspicuous zone of complete hemolysis. the medium under question is blood agar prepared from the columbia blood agar base and 5% washed sheep erythrocytes sensitised with equi factor (ef) of rhodococcus equi. optimally, 10 activity units (au) of ef per 1 ml were used. ef was titrated on a non-nutrient medium consisting o ...200011040494
cholesterol-binding cytolytic protein toxins.cholesterol-binding cytolysins (cbcs) are a large family of 50- to 60-kda single-chain proteins produced by 23 taxonomically different species of gram-positive bacteria from the genera streptococcus, bacillus, clostridium, listeria and arcanobacterium. apart pneumolysin, which is an intracytoplasmic toxin, all the other toxins are secreted in the extracellular medium. among the species producing cbcs, only l. monocytogenes and l. ivanovii are intracellular pathogens which grow and release their ...200011111910
relationship between intra-uterine bacterial contamination, endotoxin levels and the development of endometritis in postpartum cows with dystocia or retained placenta.a study was conducted to investigate the relationship between intra-uterine bacterial contamination, endotoxin levels and the development of endometritis in cows that experienced a dystocia or retained their placenta. fifteen healthy cows, 31 cows with retained placenta (rp) and 13 cows that had dystocia were clinically examined 1 or 2 days after parturition when a uterine swab for bacteriological examination was taken. in addition, plasma and uterine lochia samples were collected to determine l ...200011131320
comparison of the e test and agar dilution methods for susceptibility testing of arcanobacterium haemolyticum. 200011152320
antimicrobial susceptibility of arcanobacterium pyogenes isolated from cattle and pigs.the minimum inhibitory concentrations (mics) of 18 antimicrobial agents were determined for 49 arcanobacterium pyogenes isolates (42 bovine isolates and 7 porcine isolates). benzylpenicillin and ampicillin were the most active antibiotics, with mic ranges of < or = 0.0125-0.05 microgram/ml for both bovine and porcine isolates. all isolates were susceptible to penicillins and cephems. mics for 90% of the isolates of dihydrostreptomycin, gentamicin and oxytetracycline for bovine isolates were > 10 ...200010763384
induction of parturition with prostaglandin f2 alpha as a possible model to study impaired reproductive performance in the dairy cow.parturitions were induced in five cows, 2 weeks before term using prostaglandin (pg) f(2alpha). two i.m. injections were performed with an interval of 24 h. all cows calved within 5 days (average 2.7 days) after the first injection of pgf(2alpha). out of five cows, four had retained fetal membranes (rfm). each animal was sampled for bacteriological examination using uterine biopsies twice a week during 42 days postpartum (pp). jugular vein blood samples were withdrawn for pgf(2alpha)-metabolite ...200010837974
genetic and biochemical properties of a hemolysin (pyolysin) produced by a swine isolate of arcanobacterium (actinomyces) pyogenes.arcanobacterium (actinomyces) pyogenes, a causative agent of various pyogenic diseases in domestic animals, produces a hemolysin which is thought to be an important virulence factor. this hemolysin was purified from the culture supernatant of a. pyogenes swine isolate. the purified hemolysin showed a single band with a molecular mass of 56 kda on sds-polyacrylamide gel electrophoresis, and its isoelectric point was 9.2. the activity of this hemolysin was not enhanced by the addition of l-cystein ...200010711593
outcome of clinical mastitis in dairy heifers assessed by reexamination of cases one month after treatment.heifers that were treated for clinical mastitis prior to parturition or within 14 d postpartum were reexamined approximately 1 mo after treatment. clinical examination of the heifers and microbiological examination of quarter milk samples were carried out on both occasions. of the 1000 heifers included in the study, 10.9% were culled within 28 d after treatment. udder damage caused by mastitis was the only or main reason for culling in 96% of those heifers. in comparison, 4.5% of nonmastitic hei ...200010659966
actinomyces marimammalium sp. nov., from marine mammals.three strains of a previously undescribed actinomyces-like bacterium were isolated from samples taken from two dead seals and a porpoise. biochemical testing and page analysis of whole-cell proteins indicated the strains were phenotypically similar to each other but different from previously described actinomyces and arcanobacterium species. comparative 16s rrna gene sequencing studies showed the organisms from marine animals were genetically closely related and represent a hitherto unknown subl ...200111211252
arcanobacterium pluranimalium sp. nov., isolated from porpoise and deer.two strains of a previously undescribed arcanobacterium-like bacterium were isolated from a dead harbour porpoise and a dead sallow deer. biochemical testing and page analysis of whole-cell proteins indicated that the strains were phenotypically closely related to each other and distinct from previously described actinomyces and arcanobacterium species. comparative 16s rrna gene sequencing studies showed the bacterium to be a hitherto unknown subline within the genus arcanobacterium. based on ph ...200111211273
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