Publications

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induction of leukosis tumors with the beetle alphitobius diaperinus. 19666007732
infection and quantitative recovery of salmonella typhimurium and escherichia coli from within the lesser mealworm, alphitobius diaperinus (panzer). 19684887084
[the role of grain-mould-beetle alphitobius diaperinus in spreading of infectious bronchitis in chickens]. 19705475910
[effect of varous insecticides on the corn mold beetle (alphitobius diaperinus)]. 19724563869
effect of temperature and relative humidity on the development and mortality of alphitobius diaperinus (panz), a stored product pest. 19734733341
evaluations of sampling methods for darkling beetles (alphitobius diaperinus) in the litter of turkey and broiler houses.materials placed on the litter in turkey and broiler houses were evaluated as sampling devices for the larvae and adults of alphitobius diaperinus (lesser mealworm or darkling beetle). insects harbored in, on, and between pieces of the materials were counted after 1-week exposure. pan traps consisting of two stacked pieces of 1.3-cm thick foil-covered polyisocyanurate insulation (celotex) placed under a protective metal pan staked to the litter surface was a more effective sampling device than p ...19846531324
efficacies of mixtures of disinfectants and insecticides.efficacies of mixtures of diluted commercial formulations of selected insecticides and disinfectants were evaluated. insecticides tested included representative pyrethroids (fenvalerate [ectrin wdl and wd] and permethrin [ectiban ec]), organophosphates (dichlorvos [vapona ec], tetrachlorvinphos [rabon wp] and dichlorvos/tetrachlorvinphos [ravap ec], and a carbamate (carbaryl [sevin s]). disinfectants tested included representatives of cresylic acid (biolene), cresylic acid/phenol (bioguard x-185 ...19873112756
field trials of steinernema feltiae (nematoda: steinernematidae) for control of alphitobius diaperinus (coleoptera: tenebrionidae)in commercial broiler and turkey houses. 19873571698
occupational sensitivity to alphitobius diaperinus (panzer) (lesser mealworm).alphitobius diaperinus is an important beetle in the grain and poultry industries. we evaluated three individuals with work-related symptoms of asthma, rhinitis, conjunctivitis, urticaria, and angioedema on exposure to the insect. prick skin tests with extracts prepared from the larval, pupal, and adult life stages were positive in all three patients. specific ige antibodies to these extracts were demonstrated by rast or radioimmunoassay. rast and radioimmunoassay inhibition confirmed the specif ...19882462582
occupational sensitivity to tenebrio molitor linnaeus (yellow mealworm).tenebrio molitor is an abundant stored-grain pest in the northern united states. we evaluated an individual with work-related symptoms of rhinoconjunctivitis on exposure to this insect. prick skin tests with extracts prepared from the larval, pupal, and adult-life stages were positive for the patient and for another individual with allergy to a closely related species of beetle, alphitobius diaperinus. specific ige antibodies to the extracts were demonstrated by rast. rast inhibition demonstrate ...19902384648
use of ivermectin to control the lesser mealworm (coleoptera: tenebrionidae) in a simulated poultry broiler house.broiler chickens were fed ivermectin at a level of 2 ppm for 5 wk to determine its efficacy against the lesser mealworm, alphitobius diaperinus. this treatment essentially eliminated lesser mealworm larvae; no ivermectin residues were found in the livers of the treated chickens, even without a withdrawal period. however, the feeding of ivermectin may reduce body weights body weight gain and feed efficiency at older ages.19902235843
plasmid profiles and phage types of salmonella typhimurium isolated from successive flocks of chickens on three parent stock farms.three-hundred-and-eighty-seven strains of salmonella typhimurium obtained from successive generations of parent stock originating from three different rearing farms were characterized by phage typing and plasmid profiling. seventy-six strains representing dominant types were selected for restriction enzyme analysis and colony hybridization. the main phage type on each of the three farms was 110. plasmid profiling, however, allowed further subtyping. all but three isolates carried the serotype-sp ...199218670975
feeding behavior and growth of turkey poults fed larvae of the darkling beetle, alphitobius diaperinus.experiments were conducted to determine the effects of feeding larvae of the darkling beetle (lesser mealworm), alphitobius diaperinus (panzer) [coleoptera:tenebrionidae], to turkey poults on poult growth and of beak trimming on poult feeding on the larvae. young turkey poults readily fed on the larvae and exhibited reduced growth in the absence of other feed. poults 3 to 5 d old restricted to a diet of only larvae consumed 259 +/- 99 (+/- sd) larvae per poult per day and their body weights were ...19947816727
prevalence of protozoan infections in darkling beetles from poultry houses in north carolina.a study was conducted from november 1990 to february 1992 on the prevalence of protozoan infections in the darkling beetle, alphitobius diaperinus (coleoptera, tenebrionidae), from turkey and broiler houses in the southeastern, northeastern, and central piedmont regions of north carolina. darkling beetles were commonly infected with the eugregarine gregarina alphitobii, an undescribed species of gregarina (eugregarinorida, gregarinidae) and the neogregarine farinocystis tribolii (neogregarinorid ...19948021524
reservoir competence of the lesser mealworm (coleoptera: tenebrionidae) for salmonella typhimurium (eubacteriales: enterobacteriaceae).the reservoir competence of the lesser mealworm, alphitobius diaperinus (panzer) is reported for salmonella typhimurium (loeffler) relative to broiler chicken production. salmonella typhimurium was isolated from feces of the adult lesser mealworm at least 28 d after feeding for 24 h on 1 g of chicken feed inoculated with 3 x 10(8) bacteria/ml. all larvae fed s. typhimurium ceased voiding the bacteria in their feces before pupal molt, except one. one beetle continued to void s. typhimurium after ...19948057309
analysis of divergence of alphitobius diaperinus satellite dna--roles of recombination, replication slippage and gene conversion.satellite dna is highly abundant in alphitobius diaperinus (tenebrionidae, coleoptera), comprising 25% of the total genomic dna. sequence analysis reveals an average gc content of 50.8% and the presence of three different groups of satellite monomer variants, th1, th2 and th3 with corresponding lengths of 123, 128 and 126 bp. their mutual homologies range between 65 and 81%. sequence comparison shows that the monomer variant th2 has been formed by a recombination process between th1 and th3, whi ...19948107677
uniform distribution of satellite dna variants on the chromosomes of tenebrionid species alphitobius diaperinus and tenebrio molitor.the chromosomes of tenebrionid species alphitobius diaperinus contain large blocks of pericentromerically located constitutive heterochromatin, as revealed by c-banding procedure. as previously reported, satellite dna of this species is composed of two related monomeric units organized in three satellite subfamilies. in order to analyze the chromosomal location of the satellite dna and the distribution of monomeric variants within it, and compare it with the distribution of monomer variants in t ...19958598348
contribution of the lesser mealworm beetle (alphitobius diaperinus) to carriage of salmonella enteritidis in poultry. 19958545940
isolation of infectious bursal disease virus from the lesser mealworm, alphitobius diaperinus (panzer).infectious bursal disease virus (ibdv) was isolated from adult lesser mealworms, alphitobius diaperinus (panzer), up to 14 d after exposure, but isolation of the virus was erratic over this period of time. the virus was undetected after 24 h in beetle larvae. virus was isolated from the adult beetle's mouth parts, foregut, midgut, hindgut, and blood 24 h after they fed on feed inoculated with ibdv. ten days after exposure, virus was isolated from the foregut but not the blood, mouth parts, or re ...19957899212
feeding behavior and growth of broiler chicks fed larvae of the darkling beetle, alphitobius diaperinus.experiments were conducted to determine the effects of feeding larvae of the darkling beetle (lesser mealworm), alphitobius diaperinus (panzer) (coleoptera: tenebrionidae) to broiler chicks on chick growth. chicks readily fed on the larvae and exhibited reduced growth in the absence of other feed. chicks 3 to 8 d old restricted to a diet of only larvae consumed 1,552 +/- 172 (mean +/- sd) larvae per chick per day and their body weights were significantly less (mean = 84 g) at the end of the 6 d ...19957724456
reservoir competence of alphitobius diaperinus (coleoptera:tenebrionidae) for escherichia coli (eubacteriales:enterobacteriaceae).larval and adult lesser mealworms, alphitobius diaperinus (panzer), were found to harbor a congo red-binding strain of escherichia coli (migula) castellani & chalmers both on the external surface of their body and internally for 12 d. thereafter, e. coli was not detected, even though the beetles were exposed continuously to a food source inoculated with the bacteria. lesser mealworm larvae and adults discharge e. coli bacteria in their feces for up to 6 and 10 d, respectively. however, bacteria ...19968961651
temperature-dependent development and survival of the lesser mealworm, alphitobius diaperinus.development, growth and survival of the lesser mealworm, alphitobius diaperinus (panzer), were determined at six constant temperatures. no egg hatch or larval development occurred at 17 degrees c. at temperatures of 20, 25, 30, 35 and 38 degrees c the median development times (days), respectively, were for eggs (13.4, 6.0, 4.4, 2.6 and 2.6), larvae (133.0, 46.0, 26.2, 22.4 and 23.9), pupae (17.0, 8.0, 5.5, 4.0 and 4.1), and from oviposition to adult emergence (164.4, 60.2, 37.9, 29.0 and 30.8). ...19968834746
typhaea stercorea (coleoptera: mycetophagidae), a carrier of salmonella enterica serovar infantis in a danish broiler house.in december 1994, salmonella enterica serovar infantis (s. infantis) was accidentally introduced into a danish broiler house by stocking an s. infantis-infected broiler flock of 39,900 day-old chicks. at the time of the study, the infection had persisted through 6 broiler cycles. typhaea stercorea (l.), the hairy fungus beetle, was found in large quantities inside and around the broiler house. various attempts to control the beetle had failed; t. stercorea had been uncontrollable on the farm sin ...19989650515
differentiation of beauveria bassiana isolates from the darkling beetle, alphitobius diaperinus, using isozyme and rapd analysestwo natural genetic markers, isozymes and rapd, were utilized to differentiate 24 strains of beauveria bassiana (deuteromycotina: hyphomycetes) collected from the darkling beetle, alphitobius diaperinus (coleoptera: tenebrionidae), from poultry houses in north carolina and west virginia. nine enzyme systems were screened, of which alkaline phosphatase, alpha- and beta-esterase, and glucose phosphate isomerase gave well-resolved, scorable bands. a total of 26 isozyme bands was generated by these ...19989784340
genetic variation in beauveria bassiana populations associated with the darkling beetle, alphitobius diaperinus.a study was conducted to assess genetic variation within and among populations of beauveria bassiana (deuteromycotina: hyphomycetes) associated with the darkling beetle, alphitobius diaperinus (coleoptera: tenebrionidae), using rapd markers. a hierarchical collection of samples (strains from the same insect specimen, from insects from the same location, and from insects from different locations) was obtained from infected beetles from north carolina (nc) and west virginia (wv), usa. ten primers ...199910222180
entomopathogenic potential of verticillium and acremonium species (deuteromycotina: hyphomycetes).hyphomycetes with conidia formed in slimy heads from hyaline mycelium were isolated from a range of insect hosts. isolation on artificial medium and microscopic examination revealed that these fungi in many cases were not verticillium lecanii despite a superficial resemblance to this common entomopathogen. the fungi were identified as verticillium fusisporum, verticillium psalliotae, verticillium lamellicola, and species of acremonium. isolates of these fungi were bioassayed against the sweet-po ...199910222186
flotation method for extracting insects from poultry manure samples.we examined the efficacy of a sucrose-based flotation method for extracting 3 prevalent insect species from poultry house manure: 3rd-instar musca domestica l. (diptera: muscidae), carcinops pumilio (erichson) (coleoptera: histeridae) adults, and alphitobius diaperinus (panzer) (coleoptera: tenebrionidae) adults. a 0.6 m sucrose solution in distilled water altered the specific gravity to 1.2 and caused the 3 species to float to the water surface for easy separation. an analysis of variance (anov ...199910071504
effects of temperature and humidity on transpiration in adults of the lesser mealworm, alphitobius diaperinus (coleoptera: tenebrionidae).measurements of water loss were made on adults of the lesser mealworm, alphitobius diaperinus, using a recording micro-electrobalance and a programmable heat circulator bath. this species originates in tropical regions and infests poultry houses in temperate countries. two routes of water loss were examined: the general cuticle and via the spiracles. temperature and relative humidity of the ambient air substantially affect the cuticular transpiration in adults (fresh body weights from 12 mg to 2 ...199912770283
a sexually dimorphic response in supercooling temperature, enhanced by starvation, in the lesser mealworm alphitobius diaperinus (coleoptera: tenebrionidae).the effect of starvation on supercooling temperature (scp) distribution was investigated in adults and larvae of alphitobius diaperinus (coleoptera: tenebrionidae).the mean values for scps of adults fed at 20 degrees c were -14.5+/-2.4 degrees c (31 males) and -10.3+/-1.3 degrees c (29 females). the distribution of the scps of these control adults was unimodal. no significant differences were observed in either mean wet weight or mean dry weight between males and females.the mean values for scps ...200010880863
isolation of salmonella spp. from the housefly, musca domestica l., and the dump fly, hydrotaea aenescens (wiedemann) (diptera: muscidae), at caged-layer houses.flies, especially houseflies, are widely recognized as potential reservoirs and vectors of foodborne salmonella pathogens. in this study, flies were collected at caged-layer facilities that had produced eggs that were implicated as the food vehicle in two recent outbreaks of salmonella enteritidis infections. the flies were separated by species into pools for microbiological testing. a total of 15 species pools of houseflies, musca domestica l., and 7 species pools of bronze dump flies, hydrotae ...200010914668
limited transmission of turkey coronavirus in young turkeys by adult alphitobius diaperinus (coleoptera: tenebrionidae).we examined the role of lesser mealworm, alphitobius diaperinus (panzer), in the transmission of an enteric disease of turkeys caused by a coronavirus. turkey coronavirus (tcv) from two sources was studied, one isolate (nc95) was embryo propagated, the second was tcv infected material from turkeys diagnosed with poult enteritis mortality syndrome (pems). beetles were fed virus-infected feces mixed with chicken feed. transmission of virus was effectively halted by surface sterilization of the bee ...200015535597
mechanical barrier for preventing climbing by lesser mealworm (coleoptera: tenebrionidae) and hide beetle (coleoptera: dermestidae) larvae in poultry houses.mechanical barriers consisting of bands of polyethylene terepthalate resin attached to wooden posts by latex caulk adhesive and staples were 100% effective in preventing passage of dispersing lesser mealworm, alphitobius diaperinus (panzer), larvae in the laboratory. barriers continued to be 100% effective after beinig held in a caged layer poultry house for 3 mo. polyethylene terepthalate barriers installed on support posts in a pullet house in brooker, fl, were >92% effective against natural p ...200111777072
characterization of the larval stages of alphitobius diaperinus (panzer) (coleoptera: tenebrionidae) using head capsule width.the mean width (n = 5) of the cephalic capsule instar of alphitobius diaperinus was determined. the larvae were reared at 27 degrees c (+/- 0.1 degrees c). the result showed that a. diaperinus has eight larval instars. the head capsule of the 1st instar larvae measured x = 0.228 (sd = 0.0192) and the last instar larval measured x = 1.339 (sd = 0.0436). the developmental rate, determined by dyar-hutchinson's rule, was 1.29. these data may be useful for studies on phenology and age structure of a. ...200111340470
controlling the mealworm alphitobius diaperinus (coleoptera: tenebrionidae) in broiler and turkey houses: field trials with a combined insecticide treatment: insect growth regulator and pyrethroid.trials were conducted during one year under field conditions to control the lesser mealworm, alphitobius diaperinus (panzer), in broiler and turkey houses. the tested combined treatment included an adulticidal compound (pyrethroid: cyfluthrin) and a larvicidal compound (insect growth regulator [igr]: triflumuron). the combined insecticide treatment greatly reduced the adult and larval stocks throughout the different broiler growing periods, and control of a. diaperinus populations was achieved b ...200312650354
arthropod populations in high-rise, caged-layer houses after three manure cleanout treatments.two partial cleanout methods were compared with complete cleanouts in replicate caged-layer houses for effects on manure characteristics and subsequent dynamics in populations of manure-breeding house fly (musca domestica l.), lesser mealworm (alphitobius diaperinus [panzer]), and associated natural enemies. absolute densities of adult house flies increased by approximately two to four times within 3 wk after cleanouts of all kinds, and then remained stable over the next 3 mo. increases were lea ...200314503612
evaluation of three formulations of beauveria bassiana for control of lesser mealworm and hide beetle in georgia poultry houses.initial screening of 12 beauveria bassiana (balsamo) vuillemin isolates against larvae of the lesser mealworm (alphitobius diaperinus [panzer]) resulted in the selection of two isolates, gha and 707, for further testing under field conditions. three formulations of each strain were prepared: an ec, a ground corn granular formulation, and waste product of fungal propagation containing spent media, mycelia, and unharvested conidia ("residue" formulation). two field trials were conducted in commerc ...200314650537
the role of litter beetles as potential reservoir for salmonella enterica and thermophilic campylobacter spp. between broiler flocks.we evaluated the role of beetles infesting broiler chicken rearing facilities as potential reservoirs for salmonella enterica infections between successive broiler flocks. in addition, their role as potential reservoirs for thermophilic campylobacter spp. was also investigated. fourteen broiler houses located at 11 different farms were included in the study. the houses were nonrandomly selected on the basis of their salmonella status; nine were persistently contaminated with salmonella whereas f ...200415077793
relationship of campylobacter isolated from poultry and from darkling beetles in new zealand.campylobacter, a foodborne pathogen closely associated with poultry, is considered to be an important agent of human gastroenteritis in new zealand. the pathways involved in the contamination of poultry flocks remain unclear; however, many vectors, such as insects, rodents, and wild birds, have been implicated. infestation of poultry houses by insects, particularly darkling beetles (alphitobius diaperinus), is difficult to control. furthermore, darkling beetles are known vectors for a variety of ...200415077807
reservoir competence of lesser mealworm (coleoptera: tenebrionidae) for campylobacter jejuni (campylobacterales: campylobacteraceae).the lesser mealworm, alphitobius diaperinus (panzer), is a carrier of campylobacter spp. in poultry facilities; however, the beetle's importance in the epidemiology of campylobacteriosis is not known. a series of laboratory experiments were designed to test the vector and reservoir competence of the lesser mealworm for campylobacter jejuni. in the first experiment, c. jejuni was swabbed onto the outer surface of adult and larval beetles to determine how long bacteria can survive on the beetles' ...200515691007
lesser mealworm (coleoptera: tenebrionidae) emergence after mechanical incorporation of poultry litter into field soils.lesser mealworm, alphitobius diaperinus (panzer), emergence from north carolina field soils was evaluated in a controlled experiment simulating land application of turkey litter and again in field studies. adult lesser mealworms were buried in central north carolina cecil red clay at depths of 0, 8, 15, 23, and 30 cm and the beetles emerging from the soil counted 1, 3, 7, 10, 13, 17, 21, 24, and 28 d after burial. beetles emerged from all depths and differences among depths were not significant. ...200515765688
baseline responses of adult alphitobius diaperinus (coleoptera: tenebrionidae) to fenitrothion and susceptibility status of populations in queensland and new south wales, australia.traditionally in australia, regular applications of insecticide to the floors and lower walls of broiler houses after cleanout periods have been used in an attempt to control lesser mealworm, alphitobius diaperinus (panzer). the australian chicken meat industry has been concerned in recent years with the failure to control a. diaperinus in its broiler houses by using this method and with large beetle populations breaching farm biosecurity. resistance to fenitrothion was suspected to be responsib ...200516022325
longitudinal monitoring of two commercial layer flocks and their environments for salmonella enterica serovar enteritidis and other salmonellae.between august 20, 2001, and september 17, 2002, 1429 samples including drag swabs, egg belt or egg rollout swabs, fan-blade swabs, rodent organ and intestinal pools, beetle (alphitobius diaperinus) pools, housefly (musca domestica) pools, chicken organ and intestinal pools, and egg pools were obtained for salmonella culture from two flocks from two different commercial layer ranches. the two ranches were purposefully selected for the study based on their previous status of salmonella enteritidi ...200516094821
evaluation of a barrier to inhibit lesser mealworm (coleoptera: tenebrionidae) and dermestidae movement in high-rise, caged-layer poultry facilities.an evaluation of a mechanical barrier to prevent movement of adult and larval lesser mealworm, alphitobius diaperinus (panzer); larder beetle, dermestes lardarius l.; and hide beetle, dermestes maculatus de geer was conducted in caged-layer poultry facilities in new york and maine. the barrier, a plastic collar wrapped around building support posts, proved highly effective at preventing movement of adult lesser mealworms. significantly more lesser mealworm larvae were recovered from cardboard co ...200516334349
baseline responses of alphitobius diaperinus (coleoptera: tenebrionidae) to cyfluthrin and detection of strong resistance in field populations in eastern australia.resistance to cyfluthrin in broiler farm populations of lesser mealworm, alphitobius diaperinus (panzer) (coleoptera: tenebrionidae), in eastern australia was suspected to have contributed to recent control failures. in 2000-2001, beetles from 11 broiler farms were tested for resistance by comparing them to an insecticide-susceptible reference population by using topical application. resistance was detected in almost all beetle populations (up to 22 times the susceptible at the lc50), especially ...200616813329
resistance to cyfluthrin and tetrachlorvinphos in the lesser mealworm, alphitobius diaperinus, collected from the eastern united states.the lesser mealworm, alphitobius diaperinus (panzer), is an important pest in poultry facilities. the toxicity of cyfluthrin and tetrachlorvinphos to five strains of the lesser mealworm was compared with the toxicity to a susceptible laboratory strain. bioassays were carried out with both larvae and adults. for the susceptible strain, cyfluthrin and tetrachlorvinphos had similar toxicity to adults, but cyfluthrin was 5 times more toxic to larvae when compared with tetrachlorvinphos. high levels ...200616770758
changes in free amino acids in alphitobius diaperinus (coleoptera: tenebrionidae) during thermal and food stress.temporal changes in free amino acid pools are examined in starved and cold-exposed (10 degrees c) beetles (alphitobius diaperinus) (control beetles; 4, 7, 14, 28 and 56 days of cold-exposure). the range of individual responses and the effect of gender on survival and free amino acid levels are evaluated. females survived significantly longer (lt50 and lt90) than males under stressing conditions. pro, gln, ala, arg and thr are the major components of the free amino acid pool in the whole body of ...200616427345
survival of campylobacter spp. in darkling beetles (alphitobius diaperinus) and their larvae in australia.campylobacter infection is the most frequently reported notifiable food-borne disease in humans in australia. our studies investigated the persistence of campylobacter spp. in or on darkling beetles (alphitobius diaperinus) and their larvae. our results in analyses with chickens confirm that, unless very short turnaround times are used (<72 h), beetles colonized in one production cycle (i.e., one batch of chickens) are most unlikely to still be colonized during the next cycle of chickens.200617012593
external surface disinfection of the lesser mealworm (coleoptera: tenebrionidae).understanding the dynamics of movement of bacteria within the environment and between species is crucial to unraveling the epidemiology of bacterial diseases and to developing biosecurity measures to prevent dissemination. many arthropods, some beneficial and some detrimental, inhabit poultry houses. the lesser mealworm, alphitobius diaperinus (panzer) (coleoptera: tenebrionidae), is a pest commonly found in poultry litter that can harbor pathogens involved in both human and animal health issues ...200617017229
[selection of beauveria bassiana (bals.) vuill. and metarhizium anisopliae (metsch.) sorok. isolates against alphitobius diaperinus (panzer) (coleoptera: tenebrionidae)].this study was carried out to evaluate entomopathogenic fungi isolates as microbial control agents ofthe lesser mealworm, alphitobius diaperinus (panzer). larvae and adults were inoculated with conidial suspension of 99 isolates of beauveria bassiana (bals.) vuill. and metarhizium anisopliae (metsch.) sorok. (10(5) to 10(9) conidia/ml). vegetative growth on culture media and sporulation on culture media, cooked rice and lesser mealworm cadavers were also evaluated. isolates of b. bassiana were m ...200617348135
[effect of temperature and poultry litter in beauveria bassiana (bals.) vuill. and metarhizium anisopliae (metsch) virulence against the lesser mealworm alphitobius diaperinus (panzer) (coleoptera: tenebrionidae)].this study was carried out to evaluate the influence of temperature and poultry litter on germination vegetative growth virulence and conidial production of beauveria bassiana (bals.) vuill. and metarhizium anisopliae (metsch.) isolates on larvae and adults of the lesser mealworm (alphitobius diaperinus) (panzer). the vegetative growth and conidial production were evaluated on culture media. virulence was studied submerging larvae and adults in a conidial suspension (1 x 10(8) conidia/ml). all t ...200617352072
a preliminary study of natural and experimental infection of the lesser mealworm alphitobius diaperinus (coleoptera: tenebrionidae) with histomonas meleagridis (protozoa: sarcomastigophora).histomonas meleagridis is a protozoan parasite causing histomoniasis (histomonosis), a disease of gallinaceous fowl. in order to determine whether the lesser mealworm, alphitobius diaperinus, is capable of harbouring h. meleagridis, the presence of the parasite was tested by polymerase chain reaction in lesser mealworms collected in the field. parasite dna was detected in two larvae from two farms undergoing an outbreak of histomoniasis. insects were also artificially infected, killed after incu ...200717620173
distributions of lesser mealworm (coleoptera: tenebrionidae) in litter of a compacted earth floor broiler house in subtropical queensland, australia.distributions of lesser mealworm, alphitobius diaperinus (panzer) (coleoptera: tenebrionidae), in litter of a compacted earth floor broiler house in southeastern queensland, australia, were studied over two flocks. larvae were the predominant stage recorded. significantly low densities occurred in open locations and under drinker cups where chickens had complete access, whereas high densities were found under feed pans and along house edges where chicken access was restricted. for each flock, le ...200717849862
baseline responses of alphitobius diaperinus (coleoptera: tenebrionidae) to spinosad, and susceptibility of broiler populations in eastern and southern australia.spinosad was proposed as a potential chemical for control of lesser mealworm, alphitobius diaperinus (panzer) (coleoptera: tenebrionidae), in australian broiler houses after the detection of strong cyfluthrin resistance in many beetle populations. in 2004-2006, spinosad susceptibility of 13 beetle populations from eastern and southern australian broiler houses and a cyfluthrin/fenitrothion-resistant reference population was determined using topical application, and was compared with the suscepti ...200717849897
populational fluctuation and spatial distribution of alphitobius diaperinus (panzer) (coleoptera; tenebrionidae) in a poultry house, cascavel, parana state, brazil.knowledge of the population fluctuation and spatial distribution of pests is fundamental for establishing an appropriate control method. the population fluctuation and spatial distribution of the alphitobius diaperinus in a poultry house in cascavel, in the state of parana, brazil, was studied between october, 2001 and october 2002. larvae and adults of the lesser mealworm were sampled weekly using arends tube traps (n = 22) for six consecutive flock grow-outs. the temperature of the litter and ...200717876430
determining the adaptation potential of entomopathogenic nematode multiplication of heterorhabditis riobravus and steinernema carpocapsae (rhabditida: heterorhabditidae, steinernematidae) in larvae of alphitobius diaperinus (coleoptera: tenebrionidae) and galleria mellonella (lepidoptera: pyralidae).our objective was to determine the mean production of entomopathogenic nematodes (epns) infective juveniles (ijs) from alphitobius diaperinus and galleria mellonella larvae and the possible morphometric changes of emergent ijs. heterorhabditis riobravus and steinernema carpocapsae nematodes were placed on 20 larvae of each host individually located in petri dishes, which were maintained in an environmental control chamber. after death, each larva was individually transferred to white traps where ...200717846789
cold exposure and associated metabolic changes in adult tropical beetles exposed to fluctuating thermal regimes.environmental stress deleteriously affects every aspect of an ectotherm's biological function. frequent exposure of terrestrial insects to temperature variation has thus led to the evolution of protective biochemical and physiological mechanisms. however, the physiological mechanisms underlying the positive impact of fluctuating thermal regimes (ftrs) on the fitness and survival of cold-exposed insects have not been studied. we have thus investigated the metabolic changes in adults of the beetle ...200717331186
insect cold tolerance and repair of chill-injury at fluctuating thermal regimes: role of ion homeostasis.adults of the bug pyrrhocoris apterus and the beetle alphitobius diaperinus developed chill-injury slower and survived longer when they were exposed to fluctuating thermal regimes (ftrs, where periods of low temperature were alternated with periods of higher temperature on a daily basis) rather than to constant low temperatures. the extracellular (haemolymph) concentrations of potassium ions increased with significantly higher rates in the insects exposed to constant low temperatures than in tho ...200717275375
selection of beauveria bassiana isolates to control alphitobius diaperinus.this paper presents results on isolates of the entomopathogenic fungus beauveria bassiana for alphitobius diaperinus (coleoptera: tenebrionidae) control. of the 30 isolates tested, four (cg 71, cg 152, unioeste 4, and unioeste 40) resulted in mortality rates >or= 40% within 10 days. these mortality rates could be considered high because of the resistance of these species to b. bassiana. tests were conducted using these isolates to estimate lc(50), mortality rate over time, vegetative growth, and ...200817869265
factors affecting localized abundance and distribution of lesser mealworm in earth-floor broiler houses in subtropical australia.factors that influence the localized abundance and distribution of lesser mealworm, alphitobius diaperinus (panzer), in litter of two compacted earth-floor broiler houses in subtropical australia were studied using various experimental manipulations. numbers of lesser mealworms substantially increased inside caged areas and under uncaged empty feed pans placed in open areas of the houses. these populations were found to be localized and independent of chicken-feed, manure, and high beetle popula ...200818330117
susceptibility of alphitobius diaperinus (coleoptera: tenebrionidae) from broiler facilities in texas to four insecticides.lesser mealworm, alphitobius diaperinus (panzer) (coleoptera: tenebrionidae), adults were collected from six eastern texas broiler facilities and examined for susceptibility to four formulated insecticides. data indicate that a. diaperinus adults exposed to filter papers treated with the label rates of the insecticides exhibit some level of recovery. approximately 20% or less a. diaperinus adults treated with tempo sc ultra (8 ml/92.9 m2, 11.8% beta-cyfluthrin) or talstar wp insecticide/miticide ...200818459414
[factors affecting diatomaceous earth effectiveness in the control of alphitobius diaperinus (panzer) (coleoptera: tenebrionidae) adults].diatomaceous earth (de) is a potential alternative to control the lesser mealworm of poultry farms alphitobius diaperinus (panzer). our study aimed to understand the role of some of the environmental and insect behavioral factors play on de effectiveness, such as the substrate (chicken food and poultry house litter), temperature and de repellent activity on lesser mealworm adults. mortality was higher at the highest temperature (32 masculinec), and it increased with de concentration (53 and 84% ...200819169561
darkling beetles (alphitobius diaperinus) and their larvae as potential vectors for the transfer of campylobacter jejuni and salmonella enterica serovar paratyphi b variant java between successive broiler flocks.broiler flocks often become infected with campylobacter and salmonella, and the exact contamination routes are still not fully understood. insects like darkling beetles and their larvae may play a role in transfer of the pathogens between consecutive cycles. in this study, several groups of beetles and their larvae were artificially contaminated with a mixture of salmonella enterica serovar paratyphi b variant java and three c. jejuni strains and kept for different time intervals before they wer ...200818791034
the acquisition and internalization of salmonella by the lesser mealworm, alphitobius diaperinus (coleoptera: tenebrionidae).in poultry broiler production facilities, it is important to understand the sources and contribution of reservoir populations of pathogens. the lesser mealworm beetle, alphitobius diaperinus (panzer), is a common pest in poultry litter that is reported to carry pathogens affecting both human and animal health. this study investigates whether the carriage of a bacterial pathogen occurs by the harboring of bacteria internally by these insects. beetles were exposed to a marker bacterium, salmonella ...200818803502
comparison of two acetylcholinesterase gene cdnas of the lesser mealworm, alphitobius diaperinus, in insecticide susceptible and resistant strains.two cdnas encoding different acetylcholinesterase (ache) genes (adace1 and adace2) were sequenced and analyzed from the lesser mealworm, alphitobius diaperinus. both adace1 and adace2 were highly similar (95 and 93% amino acid identity, respectively) with the ace genes of tribolium castaneum. both adace1 and adace2 have the conserved residues characteristic of ache (catalytic triad, intra-disulfide bonds, and so on). partial cdna sequences of the alphitobius ace genes were compared between two t ...200818163527
susceptibility of lesser mealworm (coleoptera: tenebrionidae) adults and larvae exposed to two commercial insecticides on unpainted plywood panels.the susceptibilities of adult and larval lesser mealworms, alphitobius diaperinus (panzer), to two commercially formulated insecticides, cyfluthrin and tetrachlorvinphos, were examined through exposure on treated plywood panels. lesser mealworms were collected from four caged-layer poultry farms, three in new york and one in maine. an additional strain was obtained from an infestation occurring in a cricket colony.200817912691
transmission of salmonella to broilers by contaminated larval and adult lesser mealworms, alphitobius diaperinus (coleoptera: tenebrionidae).the ability of the lesser mealworm, alphitobius diaperinus (panzer), commonly known as the darkling beetle, to transmit marker salmonella typhimurium to day-of-hatch broiler chicks was evaluated, as well as the spread to nonchallenged pen mates. in trial 1, day-of-hatch chicks were orally gavaged with 4 larval or 4 adult beetles that had been exposed to marker salmonella-inoculated feed for 72 h. in addition, chicks were gavaged with the marker salmonella in saline solution. these chicks were th ...200919096055
conjugative transfer of plasmid-located antibiotic resistance genes within the gastrointestinal tract of lesser mealworm larvae, alphitobius diaperinus (coleoptera: tenebrionidae).the frequency of conjugative transfer of antimicrobial resistance plasmids between bacteria within the gastrointestinal tract of lesser mealworm larvae, a prevalent pest in poultry production facilities, was determined. lesser mealworm larvae were exposed to a negative bacterial control, a donor salmonella enterica serotype newport strain, a recipient escherichia coli, or both donor and recipient to examine horizontal gene transfer of plasmids. horizontal gene transfer was validated post externa ...200919425825
conjugative plasmid transfer between salmonella enterica newport and escherichia coli within the gastrointestinal tract of the lesser mealworm beetle, alphitobius diaperinus (coleoptera: tenebrionidae).the objective of this study was to determine if conjugative transfer of antimicrobial resistance plasmids could occur between donor and recipient bacteria within the gastrointestinal tract of lesser meal-worm beetles, a common pest in poultry production facilities. in 3 replicate studies (n = 40 overall), beetles were allowed to feed for 2 h on brain heart infusion agar inoculated with a multidrug-resistant salmonella enterica serotype newport strain (sn11 that carried plasmid replicons a/c and ...200919590068
male-produced aggregation pheromone of the lesser mealworm beetle, alphitobius diaperinus.the lesser mealworm beetle, alphitobius diaperinus (panzer), is a widespread serious pest in poultry production facilities and is difficult to control by conventional means. although pheromone-based tools have become useful in the management of other beetle pests, no pheromone was known for a. diaperinus, and this study sought to develop basic pheromone information. volatiles were collected in the laboratory from groups of male and female a. diaperinus maintained on poultry food (chick starter m ...200919337774
vectorial competence of larvae and adults of alphitobius diaperinus in the transmission of salmonella enteritidis in poultry.the ingestion of food products originating from poultry infected with salmonella spp. is one of the major causes of food poisoning in humans. the control of poultry salmonellosis is particularly difficult since birds are asymptomatic and numerous factors may expedite the maintenance of bacteria in poultry production facilities.201019929223
novel technique for quantifying adhesion of metarhizium anisopliae conidia to the tick cuticle.the present study describes an accurate quantitative method for quantifying the adherence of conidia to the arthropod cuticle and the dynamics of conidial germination on the host. the method was developed using conidia of metarhizium anisopliae var. anisopliae (metschn.) sorokin (hypocreales: clavicipitaceae) and engorged rhipicephalus annulatus (say) (arachnida: ixodidae) females and was also verified for m. anisopliae var. acridum driver et milner (hypocreales: clavicipitaceae) and alphitobius ...201020363785
responses of susceptible and cyfluthrin-resistant broiler house populations of lesser mealworm (coleoptera: tenebrionidae) to gamma-cyhalothrin.gamma-cyhalothrin was proposed as an agent for management of lesser mealworm, alphitobius diaperinus (panzer) (coleoptera: tenebrionidae), in australian broiler houses. from 2007 to 2009, baseline susceptibility of 20 australian broiler farm beetle populations plus an insecticide-susceptible laboratory population was determined for gamma-cyhalothrin by using topical application. in addition, repeat testing and regression analyses of specific beetle populations to gamma-cyhalothrin showed that to ...201021309239
[carcinops troglodytes (erichson) (coleoptera: histeridae) preying on alphitobius diaperinus (panzer) (coleoptera: tenebrionidae) in poultry houses].we first record carcinops troglodytes (paykull) as a predator of alphitobius diaperinus (panzer) larvae in poultry houses in north and northwest regions of the state of paraná. carcinops spp. are commonly recorded as predators of dipterans, and this record in poultry houses indicates the possibility of exploiting such predator for future studies aiming the development of management strategies for a. diaperinus.201021120397
metabolic rate and oxidative stress in insects exposed to low temperature thermal fluctuations.fluctuating temperatures are a predominant feature of the natural environment but their effects on ectotherm physiology are not well-understood. the warm periods of fluctuating thermal regimes (ftrs) provide opportunities for repair leading to increased survival, but there are also indications of negative effects of warm exposure. in this study, we examined respiration and oxidative stress in adult alphitobius diaperinus exposed to ftrs and to constant low temperatures. we hypothesized that cold ...201121074633
disruption of atp homeostasis during chronic cold stress and recovery in the chill susceptible beetle (alphitobius diaperinus).this study examined the impact of fluctuating thermal regimes (ftrs) on cold tolerance of the polyphagous beetle alphitobius diaperinus. daily pulses of elevated temperatures can provide breaks in chronic cold stress, potentially allowing for physiological recovery and improving survival. perturbations in central metabolism appear to be a common physiological response in insects exposed to low temperatures. it has been suggested that energy supplies, which may be depleted during cold exposure, c ...201121596153
metabolic mechanisms only partially explain resistance to pyrethroids in australian broiler house populations of lesser mealworm (coleoptera: tenebrionidae).the susceptibility of six australian broiler house populations and an insecticide susceptible population of lesser mealworm, alphitobius diaperinus (panzer) (coleoptera: tenebrionidae), to cyfluthrin, beta-cyfluthrin, gamma-cyhalothrin, and deltamethrin was investigated. one broiler house population had equivalent susceptibility to the susceptible to beta-cyfluthrin and beta-cyhalothrin, with higher susceptibility to cyfluthrin and deltamethrin. the remaining five populations demonstrated strong ...201121510215
evaluation of salmonella movement through the gut of the lesser mealworm, alphitobius diaperinus (coleoptera: tenebrionidae).abstract aims: the lesser mealworm, alphitobius diaperinus is an important poultry pest prevalent during production that is capable of vectoring pathogens. this study was undertaken to determine the gut transit time of salmonella for biosecurity risk analysis of pathogen dispersal into the environment. methods: adult and larval a. diaperinus were exposed to two concentrations of a fluorescently labeled salmonella enterica for 15, 30, and 60 min time periods then externally disinfected to evalu ...201122022817
attractiveness of an aggregation pheromone lure and chicken droppings to adults and larvae of alphitobius diaperinus (coleoptera: tenebrionidae).the chemical cues by which lesser mealworm, alphitobius diaperinus (panzer) (coleoptera: tenebrionidae) beetles find each other are still unknown. laboratory two-choice pitfall bioassays were conducted to evaluate the attractiveness of synthetic aggregation pheromone lure to lesser mealworm adults and larvae. all components of this synthetic aggregation pheromone lure, including: (r)-(+)-limonene, (e)-beta-ocimene, (s)-(+)-linalool, (r)-(+)-daucene, and 2-nonanone were also tested singly. chicke ...201223356087
introducing alphitobius diaperinus, (insecta: tenebrionidae) as a new intermediate host of hadjelia truncata (nematoda).hadjelia truncata is a nematode that causes lesions in the gizzard lining of pigeons, which may even lead to death. the aim of this study was to introduce alphitobius diaperinus as a new intermediate host for hadjelia truncata.201223109952
field assessments of control agents for lesser mealworm (coleoptera: tenebrionidae) using litter sampling.spinosad, diatomaceous earth, and cyfluthrin were assessed on two broiler farms at gleneagle and gatton in southeastern queensland, australia in 2004-2005 and 2007-2009, respectively to determine their effectiveness in controlling lesser mealworm, alphitobius diaperinus (panzer) (coleoptera: tenebrionidae). insecticide treatments were applied mostly to earth or 'hard' cement floors of broiler houses before the placement of new bedding. efficacy of each agent was assessed by regular sampling of l ...201222812139
treatment with a neem seed extract (mitestop®) of beetle larvae parasitizing the plumage of poultry.beetles of the species alphitobius diaperinus, dermestes bicolor, and dermestes lardarius may transmit severe agents of diseases on poultry and may in addition harm as larvae the skin and feathers thus leading to severe economic losses. the present study deals with a control measurement using a neem seed extract (mitestop®) being diluted with tap water. it was shown that spraying of a 1:33 dilution kills both larvae and adults of these part-time parasites as was previously shown for other parasi ...201221750872
transient gut retention and persistence of salmonella through metamorphosis in the lesser mealworm, alphitobius diaperinus (coleoptera: tenebrionidae).this study was undertaken to determine the retention of salmonella through alphitobius diaperinus metamorphosis and its contribution, through defecation, to external contamination.201222380581
extraction and characterisation of protein fractions from five insect species.tenebrio molitor, zophobas morio, alphitobius diaperinus, acheta domesticus and blaptica dubia were evaluated for their potential as a future protein source. crude protein content ranged from 19% to 22% (dumas analysis). essential amino acid levels in all insect species were comparable with soybean proteins, but lower than for casein. after aqueous extraction, next to a fat fraction, a supernatant, pellet, and residue were obtained, containing 17-23%, 33-39%, 31-47% of total protein, respectivel ...201323993491
alphitobius diaperinus (coleoptera: tenebrionidae) susceptibility to cunila angustifolia essential oil.the lesser mealworm, alphitobius diaperinus (panzer), is an insect that lives in poultry houses, and high infestations may cause economic losses to producers. the control of this insect is usually done with insecticides; however, many of these chemicals have no effect on lesser mealworm. therefore, control alternatives are needed. the aim of this study was to evaluate the influence of cunila angustifolia (benth) oil on larvae and adults of a. diaperinus. in vitro tests used larvae and adults of ...201324191374
cold resistance in the lesser mealworm alphitobius diaperinus (panzer) (coleoptera: tenebrionidae).we have investigated cold resistance, measured by the supercooling point (scp) temperature, in life stages of the lesser mealworm, alphitobius diaperinus (coleoptera: tenebrionidae), collected in brittany poultry houses. mean scp values drastically increased during the insect ontogeny: egg (-26.1 c), first instar larvae (-21.6 c), last instar larvae (-15.5 c), pupae (-11.6 c), teneral adults (-12.0 c) and mature adults (-13.1 c). nymphal metamorphosis and adult maturation did not promote substan ...201312819832
structure-related antifeedant activity of halolactones with a p-menthane system against the lesser mealworm, alphitobius diaperinus panzer.feeding deterrent activity of synthetic halogen lactones against larvae and adults of the lesser mealworm, alphitobius diaperinus panzer, in laboratory choice and no-choice tests was studied. the compounds were synthesised from racemic and enantiomerically enriched (ee = 91-98%) cis- and trans-piperitols, which were obtained from (±)-piperitone.201424009153
application of spinosad increases the susceptibility of insecticide-resistant alphitobius diaperinus (coleoptera: tenebrionidae) to pyrethroids.the effect of spinosad exposure on the susceptibility of pyrethroid- and organophosphate-resistant populations of lesser mealworm, alphitobius diaperinus (panzer) (coleoptera: tenebrionidae), to insecticides was investigated in broiler house farm and laboratory studies. a field pyrethroid- and organophosphate-resistant population showed a 3.6-fold increase in susceptibility to gamma-cyhalothrin following spinosad treatment. overall, cyfluthrin- and fenitrothion-resistant field populations were m ...201425195452
the genus alphitobius stephens (coleoptera, tenebrionidae, alphitobiini) in africa and adjacent islands.all species of the genus alphitobius stephens, 1829 (alphitobiini reitter, 1917, subfamily tenebrioninae latreille, 1802) from africa and adjacent islands are revised. new species: alphitobius capitaneus sp. n. from kenya. new synonyms: cryptops ulomoides solier, 1851, syn. n. of alphitobius diaperinus (panzer, 1796); alphitobius rufus ardoin, 1976, syn. n. of alphitobius hobohmi koch, 1953); peltoides (micropeltoides) crypticoides pic, 1916, syn. n. of peltoides (micropeltoides) opacus (gerstae ...201425009427
effect of fermentation media on the production, efficacy, and storage stability of metarhizium brunneum microsclerotia formulated as a prototype granule.new liquid fermentation techniques for the production of the bioinsecticidal fungus metarhizium brunneum strain f-52 have resulted in the formation of microsclerotia (ms), a compact, melonized-hyphal structure capable of surviving desiccation and formulation as dry granules. when rehydrated, these ms granules germinate to produce conidia that can infect susceptible insects. fermentation media containing cottonseed or soy flours as nitrogen sources and formulated at two carbon to nitrogen ratios ...201424772537
fumigant, contact, and repellent activities of essential oils against the darkling beetle, alphitobius diaperinus.the fumigant, contact, and repellent activities of four essential oils extracted from citrus limonum (sapindales: rutaceae), litsea cubeba (laurales: lauraceae), cinnamomum cassia, and allium sativum l. (asparagales: alliaceae) against 6th instars and adults of the darkling beetle, alphitobius diaperinus (panzer) (coleoptera: tenebrionidae), one of the main pests of materials and products of juncus effuses l. (poales: juncaceae) during the storage period, were assayed, and chemical ingredients w ...201425373222
a coleopteran cadherin fragment synergizes toxicity of bacillus thuringiensis toxins cry3aa, cry3bb, and cry8ca against lesser mealworm, alphitobius diaperinus (coleoptera: tenebrionidae).the lesser mealworm, alphitobius diaperinus, is a serious cosmopolitan pest of commercial poultry facilities because of its involvement in structural damage to poultry houses, reduction in feed conversion efficiency, and transfer of avian and human pathogens. cry3aa, cry3bb, and cry8ca insecticidal proteins of bacillus thuringiensis are used to control coleopteran larvae. cadherins localized in the midgut epithelium function as receptors for cry toxins in lepidopteran, coleopteran, and dipteran ...201425218400
cadherin adcad1 in alphitobius diaperinus larvae is a receptor of cry3bb toxin from bacillus thuringiensis.bacillus thuringiensis (bt) cry proteins are used as components of biopesticides or expressed in transgenic crops to control diverse insect pests worldwide. these cry toxins bind to receptors on the midgut brush border membrane and kill enterocytes culminating in larval mortality. cadherin proteins have been identified as cry toxin receptors in diverse lepidopteran, coleopteran, and dipteran species. in the present work we report a 185 kda cadherin (adcad1) from larvae of the lesser mealworm (al ...201424225445
growth performance and feed conversion efficiency of three edible mealworm species (coleoptera: tenebrionidae) on diets composed of organic by-products.insects receive increasing attention as an alternative protein-rich food source for humans. producing edible insects on diets composed of organic by-products could increase sustainability. in addition, insect growth rate and body composition, and hence nutritional quality, can be altered by diet. three edible mealworm species tenebrio molitor l., zophobas atratus fab. and alphitobius diaperinus panzer were grown on diets composed of organic by-products originating from beer brewing, bread/cookie ...201525576652
chemical composition of alphitobius diaperinus (coleoptera: tenebrionidae) abdominal glands and the influence of 1,4-benzoquinones on its behavior.this study aimed to identify and quantify the compounds present in the abdominal glands of alphitobius diaperinus panzer, 1797 (coleoptera: tenebrionidae) and to evaluate the influence of these compounds on its behavior. the extraction of volatiles present in the abdominal glands was made by dissection (10 individuals per sex) and by air entrainment (200 insects per sex), and they were analyzed by gas chromatography-flame ionization detector, gas chromatograph-mass spectrometer, and gas chromato ...201526470358
baseline susceptibility and cross-resistance in adult and larval alphitobius diaperinus (coleoptera: tenebrionidae) collected from poultry farms in arkansas.insecticide resistance in the lesser mealworm, alphitobius diaperinus (panzer) (coleoptera: tenebrionidae), can pose a serious threat to their successful management in broiler chicken farms in arkansas. resistance and cross-resistance were determined in lesser mealworm populations collected from two broiler farms in arkansas, with different insecticide application histories. farm m was treated with insecticides over the past 10 yr, whereas farm s had no insecticidal usage history. concentration- ...201526470345
a culture method for darkling beetles, blapstinus spp. (coleoptera:tenebrionidae).darkling beetles, blapstinus spp., have become a serious pest of cucurbitaceae crops, especially in california. a culture method was sought to provide large numbers (>500) of adult beetles of known age and sex that could be used for laboratory testing when needed. a method previously developed for alphitobius diaperinus (panzer) using a diet of ground chick feed, with apple slices as a moisture source, was modified for use with blapstinus spp. and then compared with the same method substituting ...201526470223
synthesis and antifeedant activity of racemic and optically active hydroxy lactones with the p-menthane system.two racemic and two enantiomeric pairs of new δ-hydroxy-γ-lactones based on the p-menthane system were prepared from racemic and optically active cis- and trans-piperitols. the johnson-claisen rearrangement of the piperitols, epoxidation of the γδ-unsaturated esters, and acidic lactonization of the epoxy esters were described. the structures of the compounds were confirmed spectroscopically. the antifeedant activities of the hydroxy lactones and racemic piperitone were evaluated against three in ...201526132506
the ability of alphitobius diaperinus homogenates immobilized on plant sorbent to block the development of mouse parkinsonism. 201525937223
combined physical and chemical methods to control lesser mealworm beetles under laboratory conditions.the lesser mealworm beetle, alphitobius diaperinus (panzer) (coleoptera: tenebrionidae), is an important insect pest. the insect acts as a disease vector and reservoir, negatively affecting the health of birds and humans, and harming poultry husbandry. controlling the lesser mealworm is generally based on using synthetic chemical insecticides, which are sometimes ineffective, and is limited due to market concerns regarding the toxicity of chemical residues in food products. in this context, the ...201525834245
revisiting the male-produced aggregation pheromone of the lesser mealworm, alphitobius diaperinus (coleoptera, tenebrionidae): identification of a six-component pheromone from a brazilian population.the lesser mealworm, alphitobius diaperinus panzer 1797 (coleoptera: tenebrionidae), is a cosmopolitan insect pest affecting poultry production. due to its cryptic behavior, insecticide control is usually not efficient. thus, sustainable and effective methods would have an enormous and positive impact in poultry production. the aim of this study was to confirm the identity of the male-produced aggregation pheromone for a brazilian population of a. diaperinus and to evaluate its biological activi ...201627494353
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