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food, feeding rates and assimilation in woodland snails.analyses of the faeces of seven species of woodland, litterdwelling snails (marpessa laminata, clausilia bidentata, oxychilus cellarius, o. alliarius, discus rotundatus, arianta arbustorum and hygromia striolata), showed that all feed predominently on higher plant material, be it living or dead. h. striolata and a. arbustorum took more chlorophyll-containing plant material than the other species, d. rotundatus had a significant amount of fungus in its faeces, while the faeces of o. cellarius and ...197028309717
the breakdown and decomposition of sweet chestnut (castanea sativa mill.) and beech (fagus sylvatica l.) leaf litter in two deciduous woodland soils : ii. changes in the carbon, hydrogen, nitrogen and polyphenol content.changes in the carbon, hydrogen, nitrogen and polyphenol content of chestnut and beech leaves were measured during the first year after fall.chestnut leaves had an initial carbon, hydrogen and nitrogen content (by weight) of 48.71%, 5.56% and 0.77% respectively; beech had a similar carbon and hydrogen content (47.77% and 5.36%) but less nitrogen (0.56%).both leaf litter types showed percentage increases in nitrogen content during the study period but only the beech showed absolute increases in n ...197328308231
the breakdown and decomposition of sweet chestnut (castanea sativa mill.) and beech (fagus sylvatica l.) leaf litter in two deciduous woodland soils : i. breakdown, leaching and decomposition.weight losses from sweet chestnut and beech leaves, attributable to biotic and abiotic breakdown processes, leaching and microbial decomposition have been studied using tethered leaves and litter bags. the experimental sites were two adjacent areas of deciduous woodland. in one area (the castanea site) a mor-like moder humus form has developed under a stand dominated by coppiced chestnut, in the other a mull-like moder underlies a stand of coppiced beech (the fagus site).chestnut leaves in the c ...197328308230
[pharmacognostic study of the sweet chestnut (castanea sativa mill.) in yugoslavia. areas of spread in yugoslavia. 1]. 19744474110
multiple shoot-bud formation and plantlet regeneration on castanea sativa mill. seeds in culture.primordial initiation and development of shoot-buds has been accomplished by using shoots derived from chestnut (castanea sativa mill) seedlings cultured with added 6-benzylaminopurine (bap). germination of chestnut seeds in the presence of bap (4 - 40 μm) stimulated varying numbers of shoot-buds in those areas of the main axis that were favorably altered. when excised single shoots from these treated seeds were subcultured on a fresh medium containing bap (4 - 40 μm) continual shoot production ...198224259134
the pollen calendar of siena (central italy).the atmospheric pollen count at siena was carried out for 4 years (1978-1981) to determine the average quantity of airborn pollen and the families, genera or species which contribute to such pollen dispersal. the pollination period stretches from february to mid october. the highest pollination period is from may to july when the most important pollen producers are poaceae, urticaceae, pinus, quercus, oleaceae and castanea sativa. also in march there are high pollen production values due to cupr ...19836660174
[free amino acids of chestnuts from castanea sativa mill., castanea crenata sieb. and zucc., castanea molissima blume and the hybrids: castanea crenata x sativa]. 19846543488
amino acids content in germinating seeds and seedlings from castanea sativa l.during germination the chestnut (castanea sativa l.) var ecotype 33 accumulates a large amount of asparagine in the cotyledons. this compound also accumulates in the growing axis:shoots and roots. in the cotyledons, gamma-aminobutyrate (gaba) represents a major amino compound during germination and early seedling growth. in young seedlings, 35 days old, arginine predominates over the other soluble amino acids, particularly in roots. five enzymic activities involved in arginine and gaba have been ...198616664882
in vitro morphogenetic competence of basal sprouts and crown branches of mature chestnut.basal shoots of five clones of mature chestnut tree (castanea sativa mill. and c. sativa x c. crenata siebold & zucc.) had a greater capacity for in vitro establishment, multiplication and rooting than crown branches of the same trees. cultures from basal shoots were more responsive than crown-derived cultures in terms of in vitro reactivity (proportion of the explants with shoot development), the mean number of shoots formed per explant, the length of the tallest shoot in each culture, and the ...199114972897
basic endochitinases are major proteins in castanea sativa cotyledons.basic endochitinases are abundant proteins in castanea sativa mill. cotyledons. three basic chitinases were purified with molecular masses of 25, 26, and 32 kd (ch1, ch2, and ch3) and with isoelectric points between 8 and 9.5. antibodies raised against ch1 cross-reacted with ch2 and ch3. however, ch3 showed differences when compared with the other two enzymes, especially in its higher cysteine content. the size, amino acid composition, and n-terminal sequence of ch1 indicate that it is a class i ...199216653058
purification, characterization and n-terminal amino acid sequence of a new major allergen from european chestnut pollen--cas s 1.pollens from trees of the order fagales (e.g. birch, alder, hazel, and hornbeam) all contain one major allergen--the main cause for tree pollen allergy. so far the major allergens from birch (bet v 1), alder (aln g 1), hazel (cor a 1), and hornbeam (car b 1) have been characterized, showing high sequence similarity with each other (1-4). we present the molecular and immunologic characterization of cas s 1, the major allergen from the european chestnut (castanea sativa). from aqueous pollen extra ...19937504464
allergens from birch pollen and pollen of the european chestnut share common epitopes.type i allergy to pollen of the european chestnut (castanea sativa) represents a major cause of pollinosis in (sub) mediterranean areas. using sera from 14 patients with established allergy to pollen of the european chestnut, 13/14 sera (92%) showed ige-binding to a 22 kd protein, 2/14 (14%) displayed additional binding to a 14 kd protein and 1/14 (7%) bound only to the 14 kd protein of european chestnut pollen extract. two monoclonal mouse antibodies, bip 1 and bip 4, directed against different ...199310779306
the rbcl gene sequence from chestnut indicates a slow rate of evolution in the fagaceae.the nucleotide sequence was obtained for the chloroplast gene coding for the large subunit of the ribulose 1,5-bisphosphate carboxylase (rbcl) of chestnut (castanea sativa mill.), a member of the woody family fagaceae. amplification primers downstream and upstream the rbcl open reading frame are also described. by comparing with other angiosperm sequences, we show that the rate of evolution of rbcl in the family fagaceae is much slower than that observed for the families of annuals analyzed.19938405983
conductimetric analysis of the ion binding properties of three leaf extracts of chestnut (castanea sativa), eucalyptus (eucalyptus globulus) and oak (quercus robur).humic materials extracted from tree leaves of chestnut (castanea sativa), eucalyptus (eucalyptus globulus) and oak (quercus robur) were analyzed by performing conductimetric titrations. values between about 84 and 236 mus cm(-1) for the molar conductivity and between 0.42 and 0.74 for the charge distribution parameter were obtained when the concentrations of the extract are increased from 40 to 100 mg 1(-1). these variations were explained by using the counterion condensation theory, and the dis ...199518966432
interference at several temporal and spatial scales between two chestnut insects.detection of interspecific competition between insects is often sensitive to scaling. we give an example of scale-dependent interference between the weevil curculio elephas and the moth cydia splendana, which both have larvae that develop in the fruits of chestnut castanea sativa. measures at three scales were considered: chestnut, husk (with one to three fertile fruits) and tree. data come from observations in the field over 14 years, complemented by experiments done directly in trees. data on ...199628307745
oral allergy syndrome induced by chestnut (castanea sativa)oral allergy syndrome is a distinctive type of allergy to food resulting from direct contact between food and the oral mucosa. normally, it affects patients who are allergic to pollens. it can be challenged by testing for hypersensitivity to fresh fruit or vegetables in well-known associations. oral allergy syndrome rarely occurs in patients with other types of allergies, or to food not associated with pollens. only occasionally does chestnut cause hypersensitivity. there are only a few reported ...19968564626
bacterial expression of an active class ib chitinase from castanea sativa cotyledons.ch3, an endochitinase of 32 kda present in castanea sativa cotyledons, showed in vitro antifungal properties when assayed against trichoderma viride. the characterization of a cdna clone corresponding to this protein indicated that ch3 is a class ib endochitinase that is synthesized as a preprotein with a signal sequence preceding the mature polypeptide. bacterial expression of mature ch3 fused to the leader peptide of the periplasmic protein ompt resulted in active ch3 enzyme. a plate assay was ...19969002617
purification and in vitro chaperone activity of a class i small heat-shock protein abundant in recalcitrant chestnut seeds.a 20-kd protein has been purified from cotyledons of recalcitrant (desiccation-sensitive) chestnut (castanea sativa) seeds, where it accumulates at levels comparable to those of major seed storage proteins. this protein, termed cs smhsp 1, forms homododecameric complexes under nondenaturing conditions and appears to be homologous to cytosolic class i small heat-shock proteins (smhsps) from plant sources. in vitro evidence has been obtained that the isolated protein can function as a molecular ch ...19979306691
population structure and disease development of cryphonectria parasitica in european chestnut forests in the presence of natural hypovirulence.abstract the cryphonectria parasitica populations in two 6-year-old european chestnut (castanea sativa) coppices were investigated in southern switzerland over a period of 4 years. occurrence of white isolates indicating an infection with cryphonectria hypovirus, vegetative compatibility groups (vcgs), hypovirulence conversion capacity, and mating types were used to characterize the populations. sampling of randomly chosen cankers in the first year yielded 59% white isolates in one and 40% in th ...199718945153
oviposition pattern of phytophagous insects: on the importance of host population heterogeneity.frequency distributions of insect immatures per host are often fitted to contagious distributions, such as the negative binomial, to deduce oviposition pattern. however, different mechanisms can be involved for each theoretical distribution and additional biological information is needed to correctly interpret the fits. we chose the chestnut weevil curculio elephas, a pest of the european chestnut castanea sativa, as a model to illustrate the difficulties of inferring oviposition pattern from fi ...199828307782
a chestnut seed cystatin differentially effective against cysteine proteinases from closely related pests.cystatin csc, a cysteine proteinase inhibitor from chestnut (castanea sativa) seeds, has been purified and characterized. its full-length cdna clone was isolated from an immature chestnut cotyledon library. the inhibitor was expressed in escherichia coli and purified from bacterial extracts. identity of both seed and recombinant cystatin was confirmed by matrix-assisted laser desorption/ionization mass spectrometry analysis, two-dimensional electrophoresis and n-terminal sequencing. csc has a mo ...19989869428
pollen calendar of the city of salamanca (spain). aeropalynological analysis for 1981-1982 and 1991-1992.we report a study on the contents of airborne pollen in the city of salamanca (spain) aimed at establishing a pollen calendar for the city for the yearly periods of maximum concentrations, relating these with quantifiable atmospheric variables over two two-year periods with an interval of 10 years between them: 1981-82 and 1991-92. the pollen was captured with burkard spore-traps, based on hirst's volumetric method. determinations were made daily and were used to make preparations, previously st ...19989885728
heterologous expression of a plant small heat-shock protein enhances escherichia coli viability under heat and cold stress.a small heat-shock protein (shsp) that shows molecular chaperone activity in vitro was recently purified from mature chestnut (castanea sativa) cotyledons. this protein, renamed here as cshsp17. 5, belongs to cytosolic class i, as revealed by cdna sequencing and immunoelectron microscopy. recombinant cshsp17.5 was overexpressed in escherichia coli to study its possible function under stress conditions. upon transfer from 37 degrees c to 50 degrees c, a temperature known to cause cell autolysis, ...199910364403
polyphenols as potential markers to differentiate juvenile and mature chestnut shoot cultures.the phenolic contents of eight in-vitro-cultivated chestnut clones (castanea sativa mill. and c. sativa x c. crenata siebold & zucc. hybrids) were analyzed both qualitatively and quantitatively. the aim of the work was to identify potential phenolic markers of: (i) juvenile or mature state; (ii) topophysical origin; and (iii) rooting capacity. a condensed tannin was detected in mature material but not in juvenile material, indicating that it could be used as a qualitative marker. other qualitati ...199912651552
low molecular weight organic compounds of chestnut wood (castanea sativa l.) and corresponding aged brandies.oak and chestnut species have been largely used for the aging of brandies, but nowadays chestnut is rarely used. there have been no previous studies regarding the cooperage utilization of chestnut wood. this study provides, for the first time, specific information about the characterization of the northern portuguese castanea sativa wood and examines the influence of this wood and its heat treatment on the chemical composition of two-year-aged brandies, by the quantitative determination (hplc) o ...199910606567
modification of structure and digestibility of chestnut starch upon cooking: a solid state (13)c cp mas nmr and enzymatic degradation study.the modification of starch, which is the major component of the polysaccharide fraction of chestnuts (castanea sativa), has been studied from the point of view of structure and digestibility to understand the modifications induced by cooking and, specifically, by the maillard reaction. the study was carried out by enzymatic degradation kinetics, monitoring the glucose released upon time, and by solid state (13)c cp mas nmr, which has the potential of monitoring the solid state phase changes occu ...199910552765
antibacterial and allelopathic activity of extract from castanea sativa leaves.following the extraction of castanea sativa with an aqueous solution of sulfuric acid (ph 3.0), the ethyl acetate soluble fraction was tested for its antibacterial and allelopathic activity. the extract was shown to have pronounced antibacterial effects against seven of the eight strains of gram-positive and gram-negative bacteria used (mic in the range of 64-256 microg/ml and mbc in the range of 256-512 microg/ml). the active fraction was analyzed by tlc and hplc showing the presence of rutin, ...200010930721
biotic and abiotic stress can induce cystatin expression in chestnut.a cysteine proteinase inhibitor (cystatin) from chestnut (castanea sativa) seeds, designated csc, has been previously characterized. its antifungal, acaricide and inhibitory activities have allowed to involve csc in defence mechanisms. the csc transcription levels decreased during seed maturation and increased throughout germination, an opposite behavior to that shown by most phytocystatins. no inhibition of endogenous proteinase activity by purified csc was found during the seed maturation or g ...200010675539
reinvigoration of mature chestnut (castanea sativa) by repeated graftings and micropropagation.gradual reinvigoration of adult chestnut (castanea sativa m. cv. montemarano) shoots was obtained by serial grafting onto juvenile rootstocks. the phenomenon was evaluated on the basis of percentage of primary nodes regenerating axillary shoots and length and number of shoots (> 10 mm) per primary node. in vitro growth of explants from serially grafted shoots was significantly lower than that of explants from seedlings at the end of the establishment phase. only microshoots from seedlings and pl ...200012651487
chloroplast dna polymorphism reveals little geographical structure in castanea sativa mill. (fagaceae) throughout southern european countries.the distribution of haplotypic diversity of 38 european chestnut (castanea sativa mill.) populations was investigated by pcr/rflp analysis of regions of the chloroplast and mitochondrial genomes in order to shed light on the history of this heavily managed species. the rapid expansion of chestnut starting from 3000 years ago is strongly related to human activities such as agricultural practice. this demonstrates the importance of human impact, which lasted some thousands of years, on the present ...200011050545
free radical scavenging activities measured by electron spin resonance spectroscopy and b16 cell antiproliferative behaviors of seven plants.in an effort to discover new antioxidant natural compounds, seven plants that grow in france (most of them in the limousin countryside) were screened. among these plants, was the extensively studied vitis vinifera as reference. for each plant, sequential percolation was realized with five solvents of increasing polarities (hexane, chloroform, ethyl acetate, methanol, and water). free radical scavenging activities were examined in different systems using electron spin resonance (esr) spectroscopy ...200111453770
rumen fermentation and nitrogen balance of lambs fed diets containing plant extracts rich in tannins and saponins, and associated emissions of nitrogen and methane.tannins were added to experimental diets at levels of 1 and 2 g/kg dm (hydrolysable tannins; castanea sativa wood extract) and saponins at 2 and 30 mg/kg dm (sarsaponin; yucca schidigera extract). these levels were far below thresholds expected to be adverse in ruminants. effects were measured in lambs by comparison with unsupplemented control diets calculated to be either deficient (10%) or adequate in protein. the diets consisted of hay, concentrate (1:1) and extra wheat starch with increasing ...200212553689
a new pyrrole alkaloid from seeds of castanea sativa.a new pyrrole alkaloid, methyl-(5-formyl-1h-pyrrole-2-yl)-4-hydroxybutyrate (1), was isolated from sweet chestnut seeds and its structure elucidated on the basis of data from nmr spectroscopy and by comparison with synthetic analogues.200211864759
proliferation, maturation and germination of castanea sativa mill. somatic embryos originated from leaf explants.experiments were performed to determine the influence of proliferation medium on the maintenance of embryogenic competence and on repetitive embryogenesis in castanea sativa mill. somatic embryos derived from leaf explants. somatic embryo proliferation was carried out by both direct secondary embryogenesis and by the culture of nodular callus tissue originated from cotyledons of somatic embryos. both systems led to the production of cotyledonary somatic embryos on murashige and skoog proliferati ...200312763755
isolation and characterization of a cdna from quercus robur differentially expressed in juvenile-like and mature shoots.a full-length cdna clone named qrcpe (quercus robur crown preferentially expressed) that is differentially expressed during in vitro culture of mature and juvenile-like shoots of quercus robur l. was identified by differential display. the deduced amino acid sequence showed that the encoded protein is small, contains a predicted n-terminal hydrophobic signal peptide that targets the protein to the cell wall, and is rich in glycine and histidine residues. accumulation of qrcpe mrna was higher in ...200312750056
cotyledon storage proteins as markers of the genetic diversity in castanea sativa miller.this study has been to analyse the useful nut globulin proteins as a marker of the genetic diversity in castanea sativa. the evaluated populations were highly polymorphic for the globulins, being detected up to 35 polymorphic bands with a wide distribution among all the evaluated populations. taken together for populations from all the chestnut regions, about 39.3% of total allelic variation was distributed among the populations. the estimates of genetic similarity between populations were clear ...200312750777
spoilage fungi and their mycotoxins in commercially marketed chestnuts.a nationwide survey was carried out to assess mould spoilage of castanea sativa nuts sold in canadian grocery stores in 1998-99. morphological and cultural characters, along with secondary metabolite profiles derived from thin-layer chromatography, were used to sort and identify fungi cultured from nut tissue. three mycotoxigenic fungi dominated (penicillium crustosum, penicillium glabrum/spinulosum and penicillium discolor) and were isolated at frequencies of 67.1%, 18.6% and 17.7%, respectivel ...200314527787
purification of castamollin, a novel antifungal protein from chinese chestnuts.a novel antifungal protein, designated castamollin, was isolated from chinese chestnut (castanea mollisima) seeds with a procedure involving ion exchange chromatography on deae-cellulose, affinity chromatography on affi-gel blue gel, ion exchange chromatography on cm-sepharose and fplc-gel filtration on superdex 75. castamollin possessed a novel n-terminal sequence demonstrating little similarity to n-terminal sequences of castanea sativa chitinase. castamollin exhibited a molecular mass of 37kd ...200314680938
analysis of serpentinophytes from north-east of portugal for trace metal accumulation--relevance to the management of mine environment.in north-east of portugal, the serpentinized area is about 8000 ha with a characteristic geology and flora. the serpentine plant community and respective soils were analyzed to examine the trace metal budget in different tissues of the plants exhibiting resistance to trace metals. one hundred and thirty five plant species belonging to 39 families and respective soils have been analyzed for total co, cr, cu, fe, mn, ni, pb and zn. substantial amounts of ni, cr, co and mn were detected in plant ti ...200414675842
comparative mapping between quercus and castanea using simple-sequence repeats (ssrs).simple sequence repeat (ssr) markers from quercus and castanea were used for comparative mapping between quercus robur (l.) and castanea sativa (mill.). we tested the transferability of ssrs developed in quercus to castanea and vice-versa. in total, 47% (25) of the quercus ssrs and 63% (19) of the castanea ssrs showed a strong amplification product in the non-source species. from these 44 putative comparative anchor tags, 19 (15 from quercus and 4 from castanea) were integrated in two previously ...200414564395
interactive effects of elevated co(2) and mineral nutrition on growth and co(2) exchange of sweet chestnut seedlings (castanea sativa).the effects of elevated atmospheric co(2) (700 micro mol mol(-1)) and fertilization were investigated on 2-year-old sweet chestnut (castanea sativa mill.) seedlings grown outdoors in pots in constantly ventilated open-sided chambers. plants were divided into four groups: fertilized controls (+f/-co(2)), unfertilized controls (-f/-co(2)), fertilized + co(2)-treated plants (+f/+co(2)) and unfertilized + co(2)-treated plants (-f/+co(2)). dry matter accumulation and allocation were measured after on ...200414967640
determination of 21 organochlorine pesticides in tree leaves using solid-phase extraction clean-up cartridges.a method to determine 21 organochlorine pesticides (ocps) in tree leaves [chestnut (castanea sativa), hazel (corylus avellana), oak (quercus robur) and walnut tree (juglans regia)] based on microwave-assisted extraction (mae) followed by solid-phase extraction (spe) clean-up is described. after extraction with hexane:acetone (50:50), four different sorbents (florisil, tandem florisil + alumina, silica and envi-carb) were assayed for the clean-up step. pesticides were eluted with 5 ml of hexane:e ...200415641355
genetic variation in carbon isotope discrimination in six european populations of castanea sativa mill. originating from contrasting localities.the objective of this study was to evaluate the variability of physiological performances of castanea sativa mill. in relation to drought tolerance, among and within european populations coming from contrasting environmental conditions. forty-eight open-pollinated families from a stratified sample (temperature/precipitation) of six naturalized populations from spain, italy and greece were grown for one growth period under two temperature regimes (25 and 32 degrees c), in combination with two wat ...200415525413
protein cryoprotective activity of a cytosolic small heat shock protein that accumulates constitutively in chestnut stems and is up-regulated by low and high temperatures.heat shock, and other stresses that cause protein misfolding and aggregation, trigger the accumulation of heat shock proteins (hsps) in virtually all organisms. among the hsps of higher plants, those belonging to the small hsp (shsp) family remain the least characterized in functional terms. we analyzed the occurrence of shsps in vegetative organs of castanea sativa (sweet chestnut), a temperate woody species that exhibits remarkable freezing tolerance. a constitutive shsp subject to seasonal pe ...200415064380
castanea sativa mill. leaves as new sources of natural antioxidant: an electronic spin resonance study.the antioxidant potential of castanea sativa mill. leaf (sweet chestnut) was explored as a new source of active extracts. the capacity of the different fractions issued from aqueous, methanol, and ethyl acetate extracts to inhibit the stable free radical 2,2-diphenyl-1-pycryl-hydrazyl, superoxide anion, and hydroxyl radical was measured by electronic spin resonance. their scavenging potential was analyzed versus their amount of phenolic compounds. among the active fractions, the most effective o ...200515656662
endophytism of sclerotinia pseudotuberosa: pcr assay for specific detection in chestnut tissues.the endophytic behaviour of sclerotinia pseudotuberosa, cause of black rot of fruits of castanea sativa, was investigated in asymptomatic tissues of the host, including nuts, buds and bark. a nested polymerase chain reaction (pcr) assay was developed for the specific detection of s. pseudotuberosa. two specific primers, rac1 and rac2 were designed within the its1-5.8s-its2 region of rdna by comparison of sequences of 24 isolates of s. pseudotuberosa and related species. specificity of primers wa ...200515736867
[sensitization to castanea sativa pollen and pollinosis in northern extremadura (spain)].castanea sativa pollen allergy has generally been considered to be uncommon and clinically insignificant. in our geographical area (plasencia, cáceres, spain) castanea sativa pollen is a major pollen.200515946627
effect of carbon and nitrogen sources, ph and temperature on in vitro culture of several isolates of amanita caesarea (scop.:fr.) pers.several isolates were obtained from sporocarps of amanita caesarea (scop.: fr.) pers. associated with quercus suber and castanea sativa coming from the southwest of spain. culture conditions were optimized for these isolates. the largest radial growth was obtained at ph 6-7, and optimal growth temperature was 24-28 degrees c depending on the isolate. albumin bovine and nitrate produced the largest patch size diameters, but the greatest mycelium dry weight yields were obtained with ammonium. mann ...200616292570
effects of four tanniferous plant extracts on the in vitro exsheathment of third-stage larvae of parasitic nematodes.the anthelmintic properties of tanniferous plants and of their secondary metabolites represent one possible alternative to chemotherapy that is currently being explored as a means of achieving sustainable control of gastrointestinal nematodes in ruminants. previous in vivo and in vitro results suggest that tanniferous plants can have direct anti-parasitic effect against different stages of nematodes. however, the mode of action of the bioactive plant compounds remains obscure. the objectives of ...200616388690
cryopreservation of zygotic embryo axes and somatic embryos of european chestnut.this work describes experiments demonstrating the feasibility of long-term conservation of castanea sativa germplasm through cryopreservation of embryonic axes or somatic embryo clumps. between 93 % and 100 % of excised embryonic axes of recalcitrant chestnut seeds survived storage in liquid nitrogen (ln) following desiccation in a laminar flow cabinet to moisture contents of 20-24 % (on a fresh weight basis), and some 63 % subsequently developed as whole plants. desiccation to moisture contents ...200615031743
quantitative determination of callose in tree roots.the formation of callose in tree roots has been suggested as a physiological indicator of aluminum (al) toxicity. quantifying callose in the roots in forest soils, however, is hampered by the presence of autofluorescent materials in the roots that disturb the measurement of callose by fluorescence spectrophotometry. tannins in the roots cause these measurement problems. here we report on the measurement of callose in the root apices of european chestnut (castanea sativa) seedlings collected in a ...200617126734
study of the temperature effect in three chestnut (castanea sativa mill.) cultivars' behaviour.the aim of this work was to analyse the effect of temperature in three chestnut cultivars, aveleira, judia and longal. for this purpose, gas exchange, thylakoid membrane potential, photosynthetic pigment and lipid content data in july, september and october under different temperatures (31, 26 and 18 degrees c) were determined. with respect to gas exchanges, significant changes in photosynthesis rate of aveleira were observed between july and september (7mumol co2m(-2)s(-1)). in contrast, judia ...200616949958
selecting earlywood vessels to maximize their environmental signal.the anatomical features of earlywood vessels often reflect information about past climatic conditions. we examined the relationships between mean monthly temperature and mean vessel lumen area (mva) in various categories of earlywood vessels. subsets of earlywood vessels of chestnut (castanea sativa mill.) were selected from a previously reported dataset based on several progressive size-related procedures. to include all earlywood vessels, the minimum size considered was 10,000 microm2. changes ...200616815831
callose in root apices of european chestnut seedlings: a physiological indicator of aluminum stress.we determined if callose formation in 1-cm-long root apices of european chestnut (castanea sativa mill.) seedlings is affected by naturally occurring high concentrations of aluminum (al) under laboratory conditions and by low base saturation (bs) of soils under forest field conditions. under controlled conditions in the laboratory, seedlings were treated with simulated soil solutions in the presence or absence (control) of al. one day after exposing seedlings to a simulated soil solution contain ...200616414922
comparison of quantitative trait loci for adaptive traits between oak and chestnut based on an expressed sequence tag consensus map.a comparative genetic and qtl mapping was performed between quercus robur l. and castanea sativa mill., two major forest tree species belonging to the fagaceae family. oak est-derived markers (stss) were used to align the 12 linkage groups of the two species. fifty-one and 45 stss were mapped in oak and chestnut, respectively. these stss, added to ssr markers previously mapped in both species, provided a total number of 55 orthologous molecular markers for comparative mapping within the fagaceae ...200616204213
microsatellite-based characterization of the castanea sativa cultivar heritage of southern switzerland.southern switzerland has a long tradition of chestnut cultivation as a staple food. local inhabitants constantly selected varieties according to the ripening period, the type of use, and the adaptability to the territory. as a result, the panorama of chestnut varieties is very complex, as reflected by more than 120 different variety names in an area of 26,000 ha. since 1994, 47 varieties have been conserved in the chestnut germplasm of southern switzerland (css), including marroni, euro-japanese ...200718059537
antibacterial activity and phytochemical evidence for the plant origin of turkish propolis from different regions.honeybees collect propolis from practically any abundant plant source in the neighborhood of the hive, be it populus, eucalyptus, pine, sugarcane, cashew nut or orange trees. we have described that the origin plants of turkish propolis are populus sp., eucalyptus sp. and castanea sativa. in our previous study, propolis samples from middle anatolia displayed the typical pattern of "poplar" propolis: they contained pinobanksin, caffeic and ferulic acids and their esters. the propolis samples exami ...200727517836
comparison of hydrodistillation and ultrasonic solvent extraction for the isolation of volatile compounds from two unifloral honeys of robinia pseudoacacia l. and castanea sativa l.a comparative study of ultrasound-assisted extraction (use) with the mixture pentane:ether (1:2) and hydrodistillation (hd) with the same trapping mixture is presented for the isolation of volatile compounds from two unifloral honeys of robinia pseudoacacia l. and castanea sativa l. all hd isolates contained many thermally derived artefacts (especially phenylacetaldehyde with lower percentages of furfural, cis- and trans-linalool oxides and others). use method gave the most representative profil ...200717321190
earlywood vessels of castanea sativa record temperature before their formation.the aim of this study was to identify the climatic signal contained in the earlywood vessel size of the ring-porous chestnut (castanea sativa) and the physiological processes involved in the underlying mechanisms. in order to assign the encoded signal to a specific physiological process, bud phenology and vessel formation were monitored along an elevation transect and chronologies of the size of the first row of earlywood vessels were retrospectively correlated with 40 yr of early spring tempera ...200717244050
involvement of reactive oxygen species during early stages of ectomycorrhiza establishment between castanea sativa and pisolithus tinctorius.evidence for the participation of reactive oxygen species (ros) and antioxidant systems in ectomycorrhizal (ecm) establishment is lacking. in this paper, we evaluated ros production and the activities of superoxide dismutase (sod) and catalase (cat) during the early contact of the ecm fungus pisolithus tinctorius with the roots of castanea sativa (chestnut tree). roots were placed in contact with p. tinctorius mycelia, and ros production was evaluated by determining the levels of h(2)o(2) and o( ...200717216276
the use of tree-rings and foliage as an archive of volcanogenic cation deposition.tree cores (pinus nigra ssp. laricio) and leaves (castanea sativa) from the flanks of mount etna, sicily were analysed by icp-ms to investigate whether volcanogenic cations within plant material provide an archive of a volcano's temporal and spatial depositional influence. there is significant compositional variability both within and between trees, but no systematic dendrochemical correlation with periods of effusive, explosive or increased degassing activity. dendrochemistry does not provide a ...200717239510
structural characterization and cytotoxic properties of a 4-o-methylglucuronoxylan from castanea sativa.a glucuronoxylan was purified from a delignified holocellulose alkaline extract of castanea sativa (spanish chestnut) and its structure analyzed by means of ft-ir, gc of the per-trimethylsilylated methylglycoside derivatives, and 1h and 13c nmr spectroscopy. the results supported a structure based on a linear polymer of xylopyranose units linked with beta(1-->4) bonds in which, on average, one out of every six units is substituted at c-2 by a 4-o-methylglucuronic acid unit; this structure is typ ...200717253851
soil fungal communities in a castanea sativa (chestnut) forest producing large quantities of boletus edulis sensu lato (porcini): where is the mycelium of porcini?a study was conducted in a castanea sativa forest that produces large quantities of the edible mushroom porcini (boletus edulis sensu lato). the primary aim was to study porcini mycelia in the soil, and to determine if there were any possible ecological and functional interactions with other dominant soil fungi. three different approaches were used: collection and morphological identification of fruiting bodies, morphological and molecular identification of ectomycorrhizae by rdna-its sequence a ...200717359260
primary and secondary metabolite composition of kernels from three cultivars of portuguese chestnut (castanea sativa mill.) at different stages of industrial transformation.chestnut (castanea sativa) is an important basic food in rural diets and a major starch crop used in a similar way to potatoes. chestnuts are a fundamental economic resource in the "chestnut regions" not only for the fruit but also for the chestnut wood. chestnuts have become increasingly important with respect to human health, for example, as an alternative gluten-free flour source. chestnuts are also a rich source of other beneficial compounds, but there have been few studies on the compositio ...200717407304
vegetable and animal food sorts found in the gastric content of sardinian wild boar (sus scrofa meridionalis).authors report results emerging from gastric content analysis from n. 96 wild boars hunted in sardinia isle, during the hunting tide (2001-2005), from november to january. mean ph of the gastric content was 3.77 +/- 0.69. mean total capacity (tc) of each stomach was 1702 +/- 680 g. mean stuff ratio (cw/tc) between the content weight (cw) and stomachs tc was 0.45. food categories found in animal stomachs were: 19 categories of vegetal species (allium spp., arbutus unedo, arisarum vulgare, avena f ...200717516948
traditional knowledge of wild edible plants used in the northwest of the iberian peninsula (spain and portugal): a comparative study.we compare traditional knowledge and use of wild edible plants in six rural regions of the northwest of the iberian peninsula as follows: campoo, picos de europa, piloña, sanabria and caurel in spain and parque natural de montesinho in portugal.200717555572
two scarecrow-like genes are induced in response to exogenous auxin in rooting-competent cuttings of distantly related forest species.we characterized scarecrow-like genes induced by auxin in rooting-competent cuttings of two distantly related forest species (pinus radiata d. don and castanea sativa mill.) before the activation of cell division that results in adventitious root formation. the predicted protein sequences contain domains characteristic of the gras protein family and show a strong similarity to the scarecrow-like proteins, indicating conserved functions of these proteins. quantitative rt-pcr analysis showed that ...200717669736
effects of extracts from italian medicinal plants on planktonic growth, biofilm formation and adherence of methicillin-resistant staphylococcus aureus.one-third of botanical remedies from southern italy are used to treat skin and soft tissue infection (ssti). staphylococcus aureus, a common cause of ssti, has generated increasing concern due to drug resistance. many plants possess antimicrobial agents and provide effective remedies for ssti. our aim was to investigate plants from different ethnobotanical usage groups for inhibition of growth and biofilms in methicillin-resistant staphylococcus aureus (mrsa).200818556162
structural patterns of rhamnogalacturonans modulating hsp-27 expression in cultured human keratinocytes.polysaccharide extracts were obtained from chestnut bran (castanea sativa), grape marc (vitis vinifera) and apple marc (malus spp.) and fractionated by size exclusion chromatography after endopolygalacturonase degradation. compositional and linkage analyses by gc and gc-ms showed the characteristic rhamnogalacturonan structure with specific arabinan (apple marc) and type ii arabinogalactan (chestnut bran, grape marc) side chains. type ii arabinogalactan rhamnogalacturonan from chestnut bran sign ...200818560339
structural characterization and cytotoxic properties of a 4-o-methylglucuronoxylan from castanea sativa. 2. evidence of a structure-activity relationship.xylans were purified from delignified holocellulose alkaline extracts of castanea sativa (spanish chestnut) and argania spinosa (argan tree) and their structures analyzed by means of gc of their per-trimethylsilylated methylglycoside derivatives and (1)h nmr spectroscopy. the structures deduced were characteristic of a 4-o-methylglucuronoxylan (mgx) and a homoxylan (hx), respectively, with degrees of polymerization ranging from 182 to 360. in the case of mgx, the regular or random distribution o ...200818646856
fungal transcript pattern during the preinfection stage (12 h) of ectomycorrhiza formed between pisolithus tinctorius and castanea sativa roots, identified using cdna microarrays.transcriptional changes in pisolithus tinctorius leading to ectomycorrhizal formation in p. tinctorius- castanea sativa were investigated using a 12-h fungal interaction in vitro system. using a 3107-cdna clone microarray, 34 unique expressed sequence tags (ests) were found to be differentially expressed. these ests represent 14 known genes, 5 upregulated and 9 downregulated, and 20 orphan sequences. some transcripts of upregulated genes (with unknown function) were previously identified in othe ...200818836771
paenibacillus castaneae sp. nov., isolated from the phyllosphere of castanea sativa miller.a bacterial strain, designated ch-32(t), was isolated from the phyllosphere of castanea sativa in spain. phylogenetic analysis based on 16s rrna gene sequences placed the isolate in the genus paenibacillus within the same subgroup as paenibacillus xinjiangensis and paenibacillus glycanilyticus, with similarities of 96.3 and 96.8 %, respectively. dna-dna hybridization values for strain ch-32(t) with these two species were lower than 20 %. the novel isolate was a gram-variable, motile, sporulating ...200818984693
salicylic acid induces h2o2 production and endochitinase gene expression but not ethylene biosynthesis in castanea sativa in vitro model system.salicylic acid (sa), ethylene (et), and wounding are all known to influence plant defense response. experiments attempting to determine sa's relation to et biosynthesis and defense gene expression have shown conflicting results. to confront this, we developed an in vitro model system to investigate how sa affects et biosynthesis, hydrogen peroxide (h(2)o(2)) production and endochitinase gene expression in the european chestnut. et measurements of in vitro shoots indicated a critical time point f ...200817765360
overall alteration of circadian clock gene expression in the chestnut cold response.cold acclimation in woody plants may have special features compared to similar processes in herbaceous plants. recent studies have shown that circadian clock behavior in the chestnut tree (castanea sativa) is disrupted by cold temperatures and that the primary oscillator feedback loop is not functional at 4 degrees c or in winter. in these conditions, cstoc1 and cslhy genes are constantly expressed. here, we show that this alteration also affects csprr5, csprr7 and csprr9. these genes are homolo ...200818958171
in vivo skin irritation potential of a castanea sativa (chestnut) leaf extract, a putative natural antioxidant for topical application.topical application of natural antioxidants has proven to be effective in protecting the skin against ultraviolet-mediated oxidative damage and provides a straightforward way to strengthen the endogenous protection system. however, natural products can provoke skin adverse effects, such as allergic and irritant contact dermatitis. skin irritation potential of castanea sativa leaf ethanol:water (7:3) extract was investigated by performing an in vivo patch test in 20 volunteers. before performing ...200818793273
an oxidative burst of superoxide in embryonic axes of recalcitrant sweet chestnut seeds as induced by excision and desiccation.recalcitrant seeds are intolerant of desiccation and cannot be stored in conventional seed banks. cryopreservation allows storage of the germplasm of some recalcitrant seeded species, but application to a wide range of plant diversity is still limited. the present work aimed at understanding the stresses that accompany the first steps in cryopreservation protocols, wounding and desiccation, both of which are likely to lead to the formation of reactive oxygen species (ros). extracellular ros prod ...200818452494
protective effect of castanea sativa and quercus robur leaf extracts against oxygen and nitrogen reactive species.topical natural antioxidants are a useful strategy for the prevention of photoaging and oxidative stress mediated skin diseases. in view of this underlying principle, the screening of natural plant extracts with scavenging activity for pro-oxidant reactive species is a primary requirement for the development of new topical antioxidant formulations. in the present study, an ethanol:water (7:3) extract from castanea sativa leaves and a ethanol:water (2:3) extract from quercus robur leaves were eva ...200818337113
phylogeny of phaeomollisia piceae gen. sp. nov.: a dark, septate, conifer-needle endophyte and its relationships to phialocephala and acephala.dark, septate endophytes (dse) were isolated from roots and needles of dwarf picea abies and from roots of vaccinium spp. growing on a permafrost site in the jura mountains in switzerland. two of the isolates sporulated after incubation for more than one year at 4 degrees c. one of them was a hitherto undescribed helotialean ascomycete phaeomollisia piceae gen. sp. nov., the other was a new species of phialocephala, p. glacialis sp. nov. both species are closely related to dse of the phialocepha ...200919015028
nutritional, fatty acid and triacylglycerol profiles of castanea sativa mill. cultivars: a compositional and chemometric approach.four portuguese chestnut cultivars from the "castanha da terra fria" protected designation of origin were selected: aveleira, boa ventura, judia and longal. the nutritional parameters (moisture, fat, protein, carbohydrates, ash and energy) as well as fibers (neutral detergent fiber, acid detergent fiber, acid detergent lignin and cellulose) were characterized. moisture was the major component followed by carbohydrates, protein and fat, resulting in an energetic value lower than 195 kcal/100 g of ...200919334758
ectomycorrhizal community structure in a healthy and a phytophthora-infected chestnut (castanea sativa mill.) stand in central italy.ink disease caused by phytophthora cambivora is a major disease of sweet chestnut (castanea sativa). in two c. sativa stands in central italy, one (montesanti) that is infected with p. cambivora and the trees showing symptoms of ink disease and another healthy stand (puzzella), the ectomycorrhizal (ecm) community structure was investigated. on the roots of the surviving trees of the diseased stand, 29 different ecm species were determined compared to 23 in the healthy stand. eleven ecm species w ...200919517140
in vitro anticancer potential of tree extracts from the walloon region forest.forty-eight extracts from 16 common belgian trees from the walloon region forest were evaluated for in vitro growth inhibitory activity against the human lovo colon cancer, pc3 prostate cancer, and u373 glioblastoma cell lines. our study was performed with the aim of selecting plant candidates in order to later isolate new anticancer compounds from an easily affordable tree material. extracts from alnus glutinosa (stem bark), carpinus betulus (leaves and stem bark), castanea sativa (stem bark), ...200919579185
vitamin e profile as a reliable authenticity discrimination factor between chestnut (castanea sativa mill.) cultivars.in this study, the profile of tocopherols and tocotrienols in chestnut ( castanea sativa mill.) kernel oil was evaluated. four portuguese chestnut varieties were selected: aveleira, boaventura, judia, and longal. the vitamin e determination had already been applied to similar matrices, but, to the authors' knowledge, it is the first time that chestnut kernel oil has been evaluated. the prevalent vitamer was gamma-tocopherol, often present in trace amounts in other natural products. due to the hi ...200919489539
acetylated h4 histone and genomic dna methylation patterns during bud set and bud burst in castanea sativa.the relationships between genomic dna cytosine methylation, histone h4 acetylation and bud dormancy in castanea sativa are described. acetylated h4 histone and genomic dna methylation patterns showed opposite abundance patterns during bud set and bud burst. increased and decreased methylation levels in the apical buds coincided with bud set and bud burst, respectively. intermediate axillary buds were characterized by constant levels of dna methylation during burst of apical buds and reduced fluc ...200919376609
ptsrr1, a putative pisolithus tinctorius symbiosis related receptor gene is expressed during the first hours of mycorrhizal interaction with castanea sativa roots.ptsrr1 est was previously identified in the first hours of pisolithus tinctorius and castanea sativa interaction. qrt-pcr confirmed ptsrr1 early expression and in silico preliminary translated peptide analysis indicated a strong probability that ptsrr1 be a transmembrane protein. these data stimulate the ptsrr1 gene research during ectomycorrhiza formation.200924031360
a fast and cost-effective approach to develop and map est-ssr markers: oak as a case study.expressed sequence tags (ests) are a source of simple sequence repeats (ssrs) that can be used to develop molecular markers for genetic studies. the availability of ests for quercus robur and quercus petraea provided a unique opportunity to develop microsatellite markers to accelerate research aimed at studying adaptation of these long-lived species to their environment. as a first step toward the construction of a ssr-based linkage map of oak for quantitative trait locus (qtl) mapping, we descr ...201020950475
aerobiology of sweet chestnut (castanea sativa mill.) in north-west croatia.the aims of the study were to analyse characteristics of the castanea airborne pollen and to compare aeropalynological data obtained from two sampling stations in north-west croatia. the study was conducted in zagreb and samobor during the 2003-2006 periods, using the seven-day volumetric samplers of the hirst design. in both study areas, the seasons of chestnut pollination were similar and lasted from june to the end of july, which is comparable to other european cities. a general rule was noti ...201020698123
phenolic compounds in chestnut (castanea sativa mill.) heartwood. effect of toasting at cooperage.the phenolic and tannic composition of heartwood extracts from castanea sativa mill., before and after toasting in cooperage, were studied using hplc-dad and hplc-dad/esi-ms, and some low molecular weight phenolic compounds and hydrolyzable tannins were found. the low molecular weight phenolic compounds were lignin constituents as the acids gallic, protocatechuic, vanillic, syringic, ferulic, and ellagic, the aldehydes protocatechuic, vanillic, syringic, coniferylic, and sinapic, and the coumari ...201020687564
velocity and pattern of ice propagation and deep supercooling in woody stems of castanea sativa, morus nigra and quercus robur measured by idta.infrared differential thermal analysis (idta) was used to monitor the velocity and pattern of ice propagation and deep supercooling of xylem parenchyma cells (xpcs) during freezing of stems of castanea sativa l., morus nigra l. and quercus robur l. that exhibit a macro- and ring-porous xylem. measurements were conducted on the surface of cross- and longitudinal stem sections. during high-temperature freezing exotherms (htes; -2.8 to -9.4°c), initial freezing was mainly observed in the youngest y ...201020616300
dna methylation during sexual embryogenesis and implications on the induction of somatic embryogenesis in castanea sativa miller.from anthesis to mature seed formation, burrs from cross-pollinated adult castanea sativa miller trees were characterized and seven developmental stages defined based on macro and micromorphological traits. in order to get an insight into the involvement of epigenetic mechanisms in sexual embryogenesis and to define somatic embryogenesis induction capability, global dna methylation and the somatic embryogenic competence were quantified. on cross-pollinated trees once fertilization takes place, a ...201020552230
aqueous extraction of glucuronoxylans from chestnut wood: new strategy for lignin oxidation using phthalocyanine or porphyrin/h2o2 system.hardwood 4-o-methylglucuronoxylans (mgx) are classically isolated by a delignification step, using a sodium chlorite solution, followed by alkaline extraction, an efficient, although potentially polluting process. in this work, we propose a chlorine-free environmentally-friendly process for mgx extraction from castanea sativa mill. chestnut sawdust was first delignified using metalled phthalocyanine or porphyrin in presence of hydrogen peroxide. then, mgx were easily extracted by hot water. this ...201020385490
evaluation of functional stability and batch-to-batch reproducibility of a castanea sativa leaf extract with antioxidant activity.a growing body of evidence suggests that free radicals are generated by uv irradiation being responsible for skin injury. in this regard, the topical use of formulations composed of plant extracts with antioxidant activity could represent a useful strategy for the prevention of photoaging and oxidative-stress-mediated diseases. the aim of this study was to assess the reproducibility of the extraction method and the functional stability of a castanea sativa leaf extract in view of its application ...201020066522
relationship between occurrence of phytophthora cambivora on plants in hardy ornamental nursery stocks and detection of the pathogen from water ponds.phytophthora cambivora was isolated from rooted bases and roots of castanea sativa, chamaecyparis lawsoniana and cotoneaster spp. as well as from water ponds located in hns. in the laboratory trials the species colonised leaf blades and stem parts of 3 tested plant species. differentiated reaction of tested plant species on p. cambivora was observed.201021534475
extracellular superoxide production, viability and redox poise in response to desiccation in recalcitrant castanea sativa seeds.reactive oxygen species (ros) are implicated in seed death following dehydration in desiccation-intolerant 'recalcitrant' seeds. however, it is unknown if and how ros are produced in the apoplast and if they play a role in stress signalling during desiccation. we studied intracellular damage and extracellular superoxide (o(2)(.-)) production upon desiccation in castanea sativa seeds, mechanisms of o(2)(.-) production and the effect of exogenously supplied ros. a transient increase in extracellul ...201019843255
composition of european chestnut (castanea sativa mill.) and association with health effects: fresh and processed products.chestnut fruits are highly regarded and widely consumed throughout europe, america and asia. various commercial forms are available, e.g. fresh and industrially processed. there have been various reviews on the composition of chestnut fruits but there has not been a comprehensive review of the different health benefits that this fruit can provide. this review is focused on the composition and associated health effects of european fresh chestnut (castanea sativa mill.) fruits and their home-proce ...201020564434
stop fitan: antispasmodic effect of natural extract of chestnut wood in guinea pig ileum and proximal colon smooth muscle.abstract stop fitan® [manufactured by demar snc, cesena (fc), italy, on behalf of geosilva, cesena] is a dietary supplement proposed as a co-adjuvant in the therapy of diarrhea. it is based on the bioactive purified natural extract of chestnut (castanea sativa) wood and saccharomyces boulardii, a nonpathogenic yeast strain that has been used for treatment and prevention of diarrhea. the effects of stop fitan and the purified natural extract of chestnut wood were assessed in vitro using guinea pi ...201020626243
antioxidant potential of chestnut (castanea sativa l.) and almond (prunus dulcis l.) by-products.the antioxidant properties of almond green husks (cvs. duro italiano, ferraduel, ferranhês, ferrastar and orelha de mula), chestnut skins and chestnut leaves (cvs. aveleira, boa ventura, judia and longal) were evaluated through several chemical and biochemical assays in order to provide a novel strategy to stimulate the application of waste products as new suppliers of useful bioactive compounds, namely antioxidants. all the assayed by-products revealed good antioxidant properties, with very low ...201021339136
an ecological and syntaxonomical overview of castanea sativa and a new association in turkey.castanea sativa mill. is thought to be originated from turkey. it is an important deciduous angiosperm with edible fruits and valuable wood. it has a wide distribution from east black sea to marmara and aegean regions. because of its wide distribution, c. sativa grows on different ecological, geological, climatic and edaphic condition and these results in different associations with different companion species evaluated in different upper units. in this paper, the status of turkish c. sativa for ...201020648816
the past, present and future tasks of hungarian dendrological research.hungarian dendrological research (research of living woody plants) has more than 200 years old history; the first general work by j+ínos kereszt+®ly grossinger was published in 1797. further basic works in our time yet are: forest botany by lajos fekete and s+índor m+íg+¦csy-dietz (1896); and the chorological work, distribution of trees and shrubs of sylvicultural importance in the region of hungarian state by lajos fekete and tibor blattny (1913). a few dendrologists and many botanists have hel ...201021565760
sugars profiles of different chestnut (castanea sativa mill.) and almond (prunus dulcis) cultivars by hplc-ri.sugar profiles of different almond and chestnut cultivars were obtained by high-performance liquid chromatography (hplc), by means of a refractive index (ri) detector. a solid-liquid extraction procedure was used in defatted and dried samples. the chromatographic separation was achieved using a eurospher 100-5 nh(2) column using an isocratic elution with acetonitrile/water (70:30, v/v) at a flow rate of 1.0 ml/min. all the compounds were separated in 16 min. the method was optimized and proved t ...201020033298
antiprotease activity of selected slovak medicinal plants.fifty-six methanol extracts obtained from the barks, flowers, leaves and stems of 30 slovak trees, bushes and herbs used in the traditional medicine of the small carpathians, slovakia, have been screened for antiprotease (trypsin, thrombin and urokinase) activity using chromogenic bioassay. in this study, 14 extracts showed the strong inhibition activity to protease trypsin with ic50 values below 10 microg/ml. the highest inhibition activities were observed for methanol extracts of acer platanoi ...201020225660
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