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effects of maltose and lysine treatment on coffee aroma by flash gas chromatography electronic nose and gas chromatography-mass spectrometry.arabica coffee is a subtropical agricultural product in china. coffee undergoes a series of thermal reactions to form abundant volatile profiles after roasting, so it loses a lot of reducing sugars and amino acids. adding carbonyl compounds with amino acids before roasting could ensure the nutrition and flavor of coffee. the technology is versatile for the development of coffee roasting process. this investigation evaluates the effects of combining maltose and lysine (lys) to modify coffee aroma ...028547803
n,n-dimethylpiperidinium (mepiquat) part 2. formation in roasted coffee and barley during thermal processing.previous work in model systems has demonstrated that mepiquat can be formed under typical roasting conditions from the amino acid lysine via the maillard reaction and trigonelline, the latter alkaloid serving as a methyl donor. this study shows for the first time that mepiquat is formed in low mg kg(-1) amounts during the coffee roasting process and consequently can be detected in roast and ground as well as soluble coffee up to levels of 1.4 mg kg(-1). darker roast coffees contain relatively hi ...024303872
the ovule and seed of coffea arabica l. 193617796844
identification of abscisic acid in flower buds of coffea arabica (l.).extracts of flower buds of coffea arabica (l.) collected before and after bud break contain abscisic acid. this was demonstrated using thin layer chromatography and gas chromatography combined with mass spectroscopy. abscisic acid accounts for about 75% of the inhibitory activity in the acidic extract. the possible role of abscisic acid in the dormancy of coffee flower buds is discussed.197024496937
[caffeine synthesis in fruits and tissue cultures of coffea arabica].during fruit development the relative caffeine content of the pericarp falls from 1.68% to 0.24% on a dry weight basis, but remains more or less constant in the seed (about 1.25%). on an absolute basis, the pericarp has twice as much and the seed twenty times as much caffeine at maturity as at the beginning of fruit development. tissue cultures of seed tissue (endosperm) produce caffeine and release it into the growth medium. both pericarp and endosperm fed with nah(14)co3 synthesize ring-labell ...197224473913
[studies on the transport of caffeine in the coffee plant (coffea arabica)].caffeine transport in small amounts from leaf to leaf was demonstrated by application of doubly-labelled caffeine. there was no translocation from the subtending leaves into the fruits, but heavy translocation from the pericaprp into the seed tissue could be shown. the results were confirmed by the analysis of grafts between caffeine-containing and caffeine-free species. the importance of the pericarp with respect to the caffeine content of the mature coffee bean is discussed.197224473741
2-chloroethanephosphonic acid reduces transpiration and stomatal opening in coffea arabica l.foliar sprays of 2-chloroethanephosphonic acid reduced transpiration rates per unit leaf area and stomatal apertures during the day in coffea arabica l. the effect was first detected six hours after treatment and persisted for up to five days.197424442781
[investigation on coffee and coffee-substitutes. xvii. behaviour of polysaccharide-complexes of robusta-coffee during roasting].two varieties of coffea robusta of light, medium and dark roasting degree were investigated. the results were similar to those which were published earlier concerning coffea arabica. one part of the mannane and galactane is destroyed. another part, which exists originally as a constituent of the holocellulose, is made water soluble. of this part, the mannane is found in the complex of water soluble polysaccharides, the galactane in the complex of galactoarabane.19751229720
a study of the lipid content of coffea arabica l. seeds. 19751161800
water movement in coffee seedlings (coffea arabica l.) : evaluation of various resistances.various resistances through the soil-plant-atmosphere continuum were measured in order to find which resistance is mainly responsible for the low leaf water potential reported in coffee plants, especially when water supply is plentiful. roots appeared to offer a major resistance in this pathway. at least under the experimental conditions mentioned here soil did not offer much resistance.it has been suggested that to complete the picture of various resistances further work should be carried out a ...197728308942
caffeine: its direct and indirect influence on reproduction.caffeine constitutes the active drug principle in a number of commonly consumed beverages. among north american adults, it holds the distinction as being probably the most widely utilized psychotropic drug. the compound is most often consumed in the form of coffee, which is derived from the bean of the tropical tree coffea arabica or coffea robusta. the unique pharmacologic and physiologic properties of caffeine have led to extensive research efforts, especially in the area of mutagenically and ...1977197235
[studies on coffee and coffee substitutes. xix. dependence of the quantity of a highly polymeric galactomannan on the degree of extraction coffee-extracts (author's transl)].in extracts of columbia arabica coffee the content of galactomannan precipitable with alcaline copper solution decreases slowly up to an extraction yield of 43.6%; it decreases somewhat more rapidly with higher extraction yields. an extract of angola robusta coffee showed opposite behaviour. in this case the content of galactomannan increased with the extraction yield, although not evenly. calculated on the basis of roasted coffee solids the extract of the arabica coffee had a rather limited ran ...1977878640
n2 fixation (c2h 2 reduction) by epiphylls on coffee,coffea arabica.nitrogen fixation (c2h2 reduction) by epiphylls on coffee,coffea arabica, grown in sites with different degrees of shade, was determined. coffee leaves with nitrogen-fixing epiphylls were found in all sites in approximately equal numbers. rates of c2h2 reduction were similar for all sites and throughout the year, averaging 3.21 nmoles c2h2 reduced leaf with epiphylls(-1) day(-1). apparently, neither rates of activity nor abundance of leaves with nitrogen-fixing epiphylls is related to the degree ...198024227230
antifungal properties of 1,3,7-trimethylxanthine, isolated from coffea arabica. 19807422010
coffea arabica. 19816941089
tobacco, cocoa, coffee, and ragweed: cross-reacting allergens that activate factor-xii-dependent pathways.a glycoprotein antigen has been isolated from cured tobacco leaves (tgp-l) nicotiana tabacum) and from cigarette smoke condensate (tgp-csc) to which approximately one-third of human volunteers, smokers and non-smokers, exhibit immediate cutaneous hypersensitivity. tgp-l and tgp-csc contain polyphenol haptens that activate the factor xii (hageman factor) dependent pathways of coagulation, fibrinolysis, and kinin generation in normal human plasma. the purpose of this communication is to describe t ...19817295998
[epidemiology of anthracnosis and eye spot disease, conclusions for its prognosis of occurrence and its control in the republic of cuba].the course of attacks by anthracnosis and by the eye spot disease on branches, leaves, and fruits of coffea arabica l. were investigated in cuba. the daily precipitation and the shading are most influential for both diseases. there are marked differences in the attacks between the rainy season (highest attacks) and dry season (decreased attacks). lack of shading increases the susceptibility of the plants to the eye spot disease. under the conditions in cuba, unshaded plantations exhibited an inc ...19827126133
purine alkaloid formation and co2 gas exchange in dependence of development and of environmental factors in leaves of coffea arabica l.in the leaves of coffea arabica l., purine alkaloid formation was estimated by analyzing the theobromine and caffeine content and by measuring the methylation rate of [2-(14)c]theobromine to [2-(14)c]caffeine in short-term experiments (6-24 h). at the same time, growth (in terms of dry weight and area), net photosynthesis (nps), and dark respiration were determined. during leaf development, which was considered to be terminated when nps was at a maximum (60-80 μmol g(-1) s(-1)) and dark respirat ...198224272573
caffeine hazards and their prevention in germinating seeds of coffee (coffea arabica l.).the inhibition of growth of seedlings of coffee (coffea arabica l.) exposed to 10 m m caffeine was found to occur in the rootlet: mitosis and cell plate formation were also inhibited. since concentrations of endogenous caffeine in the imbibed seed are 40-60 mm, 4-6 times as high as in the seedlings, we conclude that coffee embryos have specific means of avoiding caffeine autotoxicity. observations indicate that cell divisions in root tips start only after the latter are pushed away from the caff ...198324407803
high production of caffeine and related enzyme activities in callus cultures of coffea arabica l.callus tissue culture of coffea arabica l. cv hybrido de timor prepared from apical portions of orthotropic branches produced 49 to 92 times as much caffeine per unit weight of tissue as did the original explant. cell-free extracts made from 42 to 54-day-old callus cultures in which active biosynthesis was occurring exhibited n-methyl-n (9)-nucleoside hydrolase and n-methyltransferase enzyme activities. similar cell-free extracts exhibited selective biodegradative activity in forming urea from x ...198324257975
n-methyltransferase activities in suspension cultures of coffea arabica l.suspension cultures of coffea arabica l. are a useful source for methyltransferase preparations of high activity catalysing the transfer of methylgroups from s-adenosyl-l-methionine to 7-methylxanthine and to theobromine producing theobromine and caffeine respectively. surprisingly, these enzyme activities are not correlated with the availability of precursors during a culture cycle. they are highest in the growth phase when supply of precursors is reduced. mixed substrate experiments and time d ...198324257852
effect of aldicarb, ethoprop, and carbofuran on control of coffee root-knot nematode, meloidogyne exigua.egg hatch of meloidogyne exigua was significantly inhibited in 14 days pretreatment with aldicarb, ethoprop, or carbofnran at concentrations higher than 0.1 mug/ml; these eggs were found to delay hatch in 19 days posttreatment in ethoprop. aldicarb and carbofuran solutions at concentrations greater than 0.1 mug/ml significantly decreased the motility and the life span of the second-stage juveniles; aldicarb was more toxic than carbofuran to the nematode. in a field test, aldicarb (temik 10g), et ...198319295839
shade adaptation of photosynthesis in coffea arabica.the effect of irradiance on the rate of net photosynthesis was measured for mature leaves of coffee grown under five levels of radiation from 100% to 5% daylight. the rate of light-saturated photosynthesis per unit leaf area (pnmax) increased from 2 μmol co2 m(-2) s(-1) under 5% daylight to 4.4 μmol co2 m(-2) s(-1) under 100% daylight. the photon flux density (par, photosynthetically active radiation) needed for 50% saturation of photosynthesis, as well as the light compensation point, also incr ...198424458775
the influence of roasting procedure on the formation of mutagenic compounds in coffee.mutagenic products can be formed during the processing of food and especially as a result of heat treatment. direct acting mutagenic activity was found in extracts of instant coffee and roasted coffee beans using salmonella typhimurium ta100 in vitro. the mutagenic activities of the four pure coffee varieties examined (coffea arabica santos, coffea arabica columbia, coffee robusta indonesia, coffee robusta camerun) were within the same range. twenty milligrams per plate freeze-dried powder prepa ...19853891559
leaf water relations and maintenance of gas exchange in coffee cultivars grown in drying soil.plant water status, leaf tissue pressure-volume relationships, and photosynthetic gas exchange were monitored in five coffee (coffea arabica l.) cultivars growing in drying soil in the field. there were large differences among cultivars in the rates at which leaf water potential (psi(l)) and gas exchange activity declined when irrigation was discontinued. pressure-volume curve analysis indicated that increased leaf water deficits in droughted plants led to reductions in bulk leaf elasticity, osm ...199016667916
long term culture and caffeine production of immobilized coffee (coffea arabica) l. cells in polyurethane foam.coffee (coffea arabica l.) cells could be immobilized in polyurethane foam and subcultured repeatedly for a long time. four phases were observed for cell growth and caffeine production, i; immobilization, ii; growth, iii; caffeine production, iv; regrowth. their periods were influenced by the number of foam particles. especially in the phase iii, the immobilized cells produced a relatively large amount of caffeine in the subculture numbers 5-8 (34 cubes) when the fresh weight of the immobilized ...199024226594
carbon isotope discrimination in coffee genotypes grown under limited water supply.photosynthetic gas exchange, plant-water relations characteristics, and stable carbon isotope discrimination (delta) were evaluated for five coffea arabica l. genotypes growing under two soil moisture regimes in the field. the delta of leaf tissue was strongly correlated (r = -0.95) with inherent water use efficiency (ratio of assimilation to stomatal conductance; a/g). the variation in inherent water use efficiency (wue) among genotypes was 30% for plants irrigated weekly. the higher wue exhibi ...199016667234
plant regeneration from protoplasts of embryogenic cell suspensions of coffea arabica l. cv. caturra.coffee plants were regenerated from protoplasts isolated from embryogenic cell suspension cultures derived from somatic embryos of coffea arabica l. cv. caturra. yields of viable protoplasts ranged from 1×10(5) to 6×10(5) protoplast/g fresh weight. protoplast preparations usually contained no contaminating cells, and when present, the number of cells never exceeded 0.1% of the total. plating efficiencies of protoplast ranged from 1 to 10%. embryogenic protocolonies obtained after several subcult ...199124221671
calcium and phosphate effects on growth and alkaloid production in coffea arabica: experimental results and mathematical model.plant, mammalian, and microbial cells are commonly immobilized in calcium alginate gels for the production of valuable secondary metabolites. however, calcium ions are known to inhibit growth in various types of cells, and calcium is an integral part of such gels. therefore, an investigation was conducted to evaluate the effect of calcium on the growth and alkaloid production of a model cell-line, coffea arabica, in suspension culture before, attempting to immobilize such cells in alginate. a ki ...199118600686
biotransformation of theobromine to caffeine in suspension and polyurethane foam immobilized coffee (coffea arabica l.) cells.coffee (coffea arabica l.) cells are capable of biotransforming theobromine to caffeine. in suspension culture of b2k medium, which is the "production medium" for caffeine, biotransformation was also more efficient than in dk medium. more caffeine was finally produced than calculated based on theobromine added to the medium. on the other hand, the efficiency of the biotransformation using immobilized cells in reticulate polyurethane foam cubes as a matrix varied with the phases. the biotransform ...199124213687
effects of water deficit on flower opening in coffee (coffea arabica l.).the response of coffee (coffea arabica l.) floral buds to different water deficits followed by re-irrigation was investigated. flower opening was stimulated by irrigation after one period of water deficit if predawn leaf water potential declined below -0.8 mpa. similar stimulation of flowering was observed when less severe but more prolonged water deficits (ca. -0.3 to -0.5 mpa for two weeks) were imposed, even if water deficit was relieved by re-irrigation several times during this period. cons ...199214969864
a novel type of somatic embryogenesis in coffea arabica.a novel type of somatic embryogenesis characterized by an efficient and highly synchronized embryo formation was observed in embryogenic callus of coffea arabica initiated on murashige and skoog medium containing kinetin (4 mg/l) and 2,4-d (1 mg/l). it occurs in suspension and goes along with the suppression of "high frequency somatic embryo induction" (hfse). this is achieved by favoring during cultivation senescence-or necrosis-like processes which apparently do not impair the competence for e ...199224212872
description of pratylenchus gutierrezi n. sp. (nematoda: pratylenchidae) from coffee in costa rica.a lesion nematode, pratylenchus gutierrezi n. sp., collected from the roots of coffee in the central plateau of costa rica, is described and illustrated. its relationships to pratylenchus flakkensis, p. similis, and p. gibbicaudatus, the only other species of the genus having two head annules, males, or spermatheca with sperm, and an annulated tail terminus, is discussed. other distinctive characters are its posterior vulva (mean of 80%); its prominently rounded stylet knobs, low head, and subcy ...199219282999
survey of nematodes on coffee in hawaii.surveys of coffee fields in hawaii during 1989-1991 indicated the presence of 10 nematode species in 8 genera. after coffee was planted in fields previously in sugarcane, populations of criconemella sp. and pratylenchus zeae gradually decreased, while rotylenchulus reniformis and, in one field, meloidogyne incognita, increased in numbers. coffee is a poor host of r. reniformis, but weeds in coffee plantations may support this nematode. at present, nematodes pose no serious threat to hawaii's exp ...199219283060
separate de novo and salvage purine pools are involved in the biosynthesis of theobromine but not caffeine in leaves of coffea arabica l.in coffea arabica leaves, the purine ring of theobromine (3,7-dimethylxanthine) and caffeine (1,3,7-trimethylxanthine) is provided by de novo purine biosynthesis: (a) [14c]glycine, [14c]bicarbonate, and [14c]formate were incorporated into inosine 5[prime]- monophosphate (imp), sum of adenine nucleotides ([sigma]ade), theobromine, and caffeine; and (b) incorporation of [14c]formate into imp, [sigma]ade, theobromine, and caffeine was inhibited by azaserine, a known inhibitor of de novo purine bios ...199312232013
regulation of purine metabolism in intact leaves of coffea arabica.the capacity of coffea arabica leaves (5- x 5-mm pieces) to synthesize de novo and catabolize purine nucleotides to provide precursors for caffeine (1,3,7-trimethylxanthine) was investigated. consistent with de novo synthesis, glycine, bicarbonate, and formate were incorporated into the purine ring of inosine 5[prime]-monophosphate (imp) and adenine nucleotides ([sigma]ade); azaserine, a known inhibitor of purine de novo synthesis, inhibited incorporation. activity of the de novo pathway in c. a ...199312232012
immobilized coffea arabica cell culture using a bubble-column reactor with controlled light intensity.coffea arabica cells immobilized by calcium alginate gel were photocultured using a bubble-column reactor under controlled light intensity. this process was carried out after their alkaloid productivity was improved by increasing the cell density in the initial gel matrix and preculturing the immobilized cells in the dark prior to light irradiation. the cells were grown in the form of a biofilm on gel beads, producing 100 mg/l of purine alkaloids in a 24-day batch culture. alkaloid production wa ...199318613054
lipid content and composition of coffee brews prepared by different methods.the lipid content and composition of boiled, filtered, dripped, turkish and espresso coffees prepared from roasted beans of coffea arabica and coffea robusta, and of coffees prepared from different brands of instant coffee were examined. the lipid content varied with the method of preparation. while coffee brews filtered through filter paper contained less than 7 mg lipids, those prepared by boiling without filtering and espresso coffee reached 60-160 mg lipids/150-ml cup. coffee brew filtered t ...19938477916
canonical discriminant analysis applied to the headspace gc profiles of coffee cultivars.chemometric methods were applied for analyzing the relationship between the classification of coffee cultivars and their volatile components. six typical cultivars were selected from coffea arabica l., and their headspace profiles were analyzed by gc and gc/ms. a canonical discriminant analysis, with gc peaks as the variables, suggested the existence of a relationship between the sensory characteristics and canonical variables. the six coffee cultivars were divided into three groups, respectivel ...199327280982
detection of genetic diversity and selective gene introgression in coffee using rapd markers.rapd (randomly amplified polymorphic dna) markers generated by arbitary decamers have been successfully employed to detect genetic polymorphisms between coffee species and between coffea arabica genotypes. the rapd profiles were used to construct dendrograms and these were consistent with the known history and evolution of coffea arabica. material originating from ethiopia and the arabica sub-groups - c. arabica var. typica and c. arabica var. bourbon - were clearly distinguished. rapd analysis ...199424190527
the effect of environmentally induced stem temperature gradients on transpiration estimates from the heat balance method in two tropical woody species.commercially available sap flow gauges were used to evaluate the performance of the stem heat balance (shb) technique for measuring sap flow in coffee (coffea arabica l. cv. yellow catuai) and koa (acacia koa gray) plants under greenhouse and field conditions. transpiration rates measured gravimetrically and with the shb technique were similar in greenhouse tests, provided that insulation in addition to that supplied by the gauge manufacturer was applied to reduce radiant heating in the vicinity ...199414967712
comparative study of protein electrophoretic patterns during embryogenesis in coffea arabica cv catimor.embryogenic and non-embryogenic calli from leaf sections of coffea arabica cv. catimor, were analyzed under denaturing conditions in one- and two-dimensions by polyacrylamide gel electrophoresis. the protein patterns revealed qualitative and quantitative differences in size and charge. in non-embryogenic calli, two dimensional analysis reveals seven distinctive polypeptides in the range of 15 to 70 kda. four of the polypeptides are acidic, three of 70 kda and one of 15 kda. similarly, in embryog ...199424193651
analysis of free amino acids in green coffee beans. i. determination of amino acids after precolumn derivatization using 9-fluorenylmethylchloroformate.for the determination of free amino acids in green coffee beans, 9-fluorenylmethylchloroformate was applied successfully as the precolumn derivatization agent. the separation of the 27 free amino acids of green coffee as yet identified or quantitatively determined is almost complete. the paper describes in detail the conditions of extraction, derivatization and resolution and presents reproducible data of standard curves and of quantitative results. it has been demonstrated that by applying the ...19948067059
potential sources of sugar for the phlebotomine sandfly lutzomyia youngi (diptera: psychodidae) in a colombian coffee plantation.sugar-deprived lutzomyia youngi were exposed to each of the five most numerous plant species in a colombian coffee plantation for 24 h and then tested for the presence of fructose by the cold anthrone assay. many flies exposed to coffee (coffea arabica) (up to 55% in an individual test) and guamo (inga edulis) (up to 28.0%) gave positive results. although some flies also took sugar from red-flowered balsam (impatiens balsamina) and bamboo (bambusa angustifolia), none of the insects exposed to pl ...19947979645
diterpene composition of oils from arabica and robusta coffee beans and their effects on serum lipids in man.the cholesterol-raising effect of boiled coffee is caused by diterpenes from coffee oil. in order to identify the diterpene responsible, we analysed the diterpene composition of oils from arabica (coffea arabica) and robusta (coffea canephora robusta) beans and their effects on serum lipids and thyroid function. design, subjects, and intervention: during the first 3-week period of a randomized, cross-over trial, 11 healthy, normolipaemic volunteers received per day either 2 g of coffee oil (n = ...19957782725
investigation of the effect of coffee lipids on serum cholesterol in hamsters.the effect of coffee total lipids (ctl), coffee non-saponifiable matter (nsm) and coffee diterpene alcohols (dta) extracted from coffea arabica beans on serum cholesterol in adult male syrian hamsters was examined. the animals were fed either a commercial laboratory chow diet (study 1), containing 5% fat and low in saturated fat (1.46 g/100 g diet) and cholesterol (0.03 g/100 g diet) or a semi-synthetic diet (study 2) set in gelatine, containing 10% fat and high in saturated fat (4 g/100 g diet) ...19957896229
a placebo-controlled parallel study of the effect of two types of coffee oil on serum lipids and transaminases: identification of chemical substances involved in the cholesterol-raising effect of coffee.in a randomized double-blind parallel study in 36 subjects the effect on serum cholesterol of a daily dose of 2 g lipid extracted from green arabica and robusta coffee beans was studied. arabica oil elevated serum total cholesterol by 1.1 mmol/l (95% ci for the difference from placebo: 0.41, 1.73 mmol/l); the effect of robusta oil (+0.5 mmol/l) was not statistically significant (95% ci: -0.01, 0.92 mmol/l). arabica oil also raised plasma triglycerides by 0.8 mmol/l (95% ci: 0.26, 1.25 mmol/l). t ...19957762531
a cdna encoding a metallothionein i-like protein from coffee leaves (coffea arabica). 19957870830
biosynthesis of caffeine in leaves of coffee.the levels of endogenous caffeine and theobromine were much higher in buds and young leaves of coffea arabica l. cv kent than in fully developed leaves. biosynthesis of caffeine from 14c-labeled adenine, guanine, xanthosine, and theobromine was observed, whereas other studies (h. ashihara, a.m. monteiro, t. moritz, f.m. gillies, a. crozier [1996] planta 198: 334-339) have indicated that there is no detectable incorporation of label into caffeine when theophylline and xanthine are used as substra ...199612226327
analysis of free amino acids in green coffee beans. ii. changes of the amino acid content in arabica coffees in connection with post-harvest model treatment.to investigate amino acid changes in green coffee beans in the post-harvest period, amino acid concentrations were determined in green beans and after modelled drying, fermentation and storage. after the drying at alternating temperatures up to maximally 40 degrees c, considerable changes in the concentrations of individual amino acids were identified. at the beginning of the storage period, significant changes in concentration were found to a minor extent. under the condition of drying, it was ...19969123976
semiquantitative evaluation of dermal exposure to granulated insecticides in coffee (coffea arabica l.) crop and efficiency of individual protective equipment. 19968875843
[use of a coffea arabica tosta extract for the prevention and therapy of polyfactorial infectious diseases in newborn calves].two studies have been carried out to evaluate the prophylactic and therapeutical effect of a 30%-extract from the coffee-bean seeds coffeae arabicae on infectious diseases in newborn calves. 1. within a large cattle-herd, which endemically showed a high proportion of infections within the gastroenteric and/or respiratory systems in calves, a randomised placebo-controlled double-blind study has been done. 50 newborn calves were given a subcutaneous injection of 10 ml coffea-preparation 30% on fir ...19969035968
meloidogyne paranaensis n. sp. (nemata: meloidogynidae), a root-knot nematode parasitizing coffee in brazil.a root-knot nematode parasitizing coffee in paran state, brazil, is described as meloidogyne paranaensis n. sp. the suggested common name is paraná coffee root-knot nematode. the perineal pattern is similar to that of m. incognita; the labial disc and medial lips of the female are fused and asymmetric and rectangular; the lateral lips are small, triangular, and fused laterally with the head region. the female stylet is 15.0-17.5 mum long, with broad, distinctly set-off knobs; the distance from t ...199619277133
coffea arabica l., a new host plant for acetobacter diazotrophicus, and isolation of other nitrogen-fixing acetobacteria.acetobacter diazotrophicus was isolated from coffee plant tissues and from rhizosphere soils. isolation frequencies ranged from 15 to 40% and were dependent on soil ph. attempts to isolate this bacterial species from coffee fruit, from inside vesicular-arbuscular mycorrhizal fungi spores, or from mealybugs (planococcus citri) associated with coffee plants were not successful. other acid-producing diazotrophic bacteria were recovered with frequencies of 20% from the coffee rhizosphere. these n2-f ...19979293018
two different conductances contribute to the anion currents in coffea arabica protoplasts.the anion conductance of the plasma membrane of coffea arabica protoplasts was isolated and characterized using the whole-cell patch clamp technique. voltage pulse protocols revealed two components: a voltage-gated conductance (gs) and a voltage-independent one (gl). gs is activated upon depolarization (e-fold activation every +36 mv) with time constants of 1 sec and 5 sec at all potentials. gl and gs also differ by their kinetic and biophysical properties. in bi-ionic conditions the current ass ...19979309213
lipid analysis of coffea arabica linn. beans and their possible hypercholesterolemic effects.lipid composition of green coffea arabica beans is reported with special emphasis on fatty acid composition. triacylglycerols were found to be the major lipid constituents of the coffee oil along with sterol esters, sterols/triterpene alcohol, hydrocarbons and the hydrolyzed products of triacylglycerols as the minor components. fatty acid composition of total oil, neutral lipids, polar lipids and pure triacylglycerols showed the presence of fatty acids of c14, c16, c18, and c20 carbon chains. pa ...19979135777
chilling stress leads to increased cell membrane rigidity in roots of coffee (coffea arabica l.) seedlings.tropical and sub-tropical higher plant species show marked growth inhibition when exposed to chilling temperatures. in root tip segments of coffee seedlings which were subjected for 6 days to temperatures of 10, 15, 20 and 25 degrees c, in darkness, we have detected an increased amount of malondialdehyde formed in the 10 degrees c treatment, accompanied by higher electrolyte leakage. the electron paramagnetic resonance (epr) technique and the fatty acid spin probes 5-, 12- and 16-doxylstearic ac ...19979030214
intermittent light irradiation with a second-scale interval enhances caffeine production by coffea arabica cellswe developed novel equipment that intermittently illuminates coffea arabica cell suspensions at a second-scale interval and investigated how intermittent irradiation enhances caffeine biosynthesis by c. arabica cells. the light/dark cycles consisting of 2 s of illumination and 18 s of darkness enhanced caffeine production, reaching the same level as for continuous light. the intermittent illumination increased the production efficiency regarding light consumption by a factor of 10. caffeine prod ...19989758671
biochemistry and toxicology of the diterpenoid glycoside atractyloside.atractyloside (atr) is a diterpenoid glycoside that occurs naturally in plants (many of which are used in ethnomedicines) found in europe, africa, south america, asia and the far east. it is also present in animal grazing forage. atr (and its analogues) may be present at levels as high as 600 mg/kg dried plant material. consumption of the plants containing atr or carboxyatractyloside (carboxyatr) has caused fatal renal proximal tubule necrosis and/or centrilobular hepatic necrosis in man and far ...19989651051
isolation of a paracoccidioides brasiliensis strain from the soil of a coffee plantation in ibiá, state of minas gerais, brazil.paracoccidioides brasiliensis has rarely been isolated from its habitat in rural areas. in order to investigate the hypothesis that human infection with this fungus is linked to coffee plantations (coffea arabica), material was collected monthly over a period of 1 year from farms in the town of ibiá, state of minas gerais, brazil. a total of 760 samples of soil, coffee leaves and fruits was cultured and inoculated into mice. a fungus isolated from the liver of a mouse inoculated with soil showed ...19989776810
effects of pratylenchus brachyurus and p. coffeae on seedlings of coffea arabica.two experiments were carried out to evaluate the effects of pratylenchus brachyurus and p. coffeae on coffea arabica. the first experiment was conducted in a greenhouse to determine the effects of pratylenchus brachyurus and p. coffeae on seedlings of coffea arabica cv. mundo novo. both pratylenchus spp. reduced the growth of coffee seedlings. higher contents of soluble sugars were detected in the leaves of infected plants. the reproduction rate of p. brachyurus was very low on cv. mundo novo, i ...199819274229
survey of crop losses in response to phytoparasitic nematodes in the united states for 1994.previous reports of crop losses to plant-parasitic nematodes have relied on published results of survey data based on certain commodities, including tobacco, peanuts, cotton, and soybean. reports on crop-loss assessment by land-grant universities and many commodity groups generally are no longer available, with the exception of the university of georgia, the beltwide cotton conference, and selected groups concerned with soybean. the society of nematologists extension committee contacted extensio ...199919270925
biosynthesis and catabolism of caffeine in low-caffeine-containing species of coffea.leaves of coffea salvatrix, coffea eugenioides, and c. bengalensis contain approximately 3-7-fold lower levels of caffeine than those of coffea arabica. there was more extensive biosynthesis of caffeine from [8-(14)c]adenine in young leaves of c. arabica than in c. salvatrix, c. eugenioides, and c. bengalensis. degradation of [8-(14)c]caffeine, which is negligible in leaves of c. arabica, was also very slow in c.salvatrix and c. bengalensis. in contrast, [8-(14)c]caffeine was catabolized rapidly ...199910552667
xanthine degradation and related enzyme activities in leaves and fruits of two coffea species differing in caffeine catabolism.the degradation of xanthine was studied in young and aged leaves and in immature and mature fruits of coffea arabica and coffea dewevrei, which differ with respect to caffeine catabolism. radioisotope feeding experiments showed that leaves degraded xanthine more readily than fruits but that mature fruits and aged leaves were less efficient than younger tissues. in all cases, a significant part of the recovered radioactivity was in the ureides. xanthine dehydrogenase was characterized as the enzy ...199910552461
sensory study on the character impact odorants of roasted arabica coffee.the potent odorants were quantified in a sample of roasted arabica coffee. on the basis of the results, 27 odorants were dissolved in an oil/water mixture. the flavor profile of the model obtained was very close to that of the real sample. in duo and triangle tests, the model was compared with models missing one or more odorants. these experiments indicated that 2-furfurylthiol, 4-vinylguaiacol, several alkyl pyrazines, furanones, acetaldehyde, propanal, methylpropanal, and 2- and 3-methylbutana ...199910563955
molecular characterisation and origin of the coffea arabica l. genome.restriction fragment length polymorphism (rflp) markers were used in combination with genomic in situ hybridisation (gish) to investigate the origin of the allotetraploid species coffea arabica (2n = 44). by comparing the rflp patterns of potential diploid progenitor species with those of c. arabica, the sources of the two sets of chromosomes, or genomes, combined in c. arabica were identified. the genome organisation of c. arabica was confirmed by gish using simultaneously labelled total genomi ...199910102360
water utilization, plant hydraulic properties and xylem vulnerability in three contrasting coffee (coffea arabica) cultivars.water use, hydraulic properties and xylem vulnerability to cavitation were studied in the coffee (coffea arabica l.) cultivars san ramon, yellow caturra and typica growing in the field under similar environmental conditions. the cultivars differed in growth habit, crown morphology and total leaf surface area. sap flow, stomatal conductance (g(s)), crown conductance (g(c)), apparent hydraulic conductance of the soil-leaf pathway (g(t)), leaf water potential (psi(l)) and xylem vulnerability to los ...200012651468
the synthesis, testing and use of 5-fluoro-alpha-d-galactosyl fluoride to trap an intermediate on green coffee bean alpha-galactosidase and identify the catalytic nucleophile.5-fluoro-alpha-d-galactopyranosyl fluoride was synthesized and its interaction with the active site of an alpha-galactosidase from green coffee bean (coffea arabica), a retaining glycosidase, characterized kinetically and structurally. the compound behaves as an apparently tight binding (ki = 600 nm) competitive inhibitor, achieving this high affinity through reaction as a slow substrate that accumulates a high steady-state concentration of the glycosyl-enzyme intermediate, as evidenced by esims ...200011128583
characterization of polyphenol oxidase in coffee.polyphenol oxidase (ppo) was characterized in partially purified extracts of leaves (ppo-l) and fruit endosperm (ppo-e) of coffee (coffea arabica l.). ppo activity was higher in early developmental stages of both leaves and endosperm of fruits. wounding or exposure of coffee leaves to methyl jasmonate increased ppo activity 1.5-4-fold. ppo was not latent and was not activated by protease treatment. ppo activity was stimulated 10-15% with sodium dodecyl sulphate (sds) at 0.35-1.75 mm, but at high ...200011117875
characterization of microsatellite loci in coffea arabica and related coffee species. 200010964241
potent odorants of raw arabica coffee. their changes during roasting.aroma extract dilution analysis of raw arabica coffee revealed 3-isobutyl-2-methoxypyrazine (i), 2-methoxy-3,5-dimethylpyrazine (ii), ethyl 2-methylbutyrate (iii), ethyl 3-methylbutyrate (iv), and 3-isopropyl-2-methoxypyrazine (v) as potent odorants. the highest odor activity value was found for i followed by ii, iv, and v. it was concluded that i was responsible for the characteristic, peasy odor note of raw coffee. twelve odorants occurring in raw coffee and (e)-beta-damascenone were also quan ...200010725165
in vitro antioxidant and ex vivo protective activities of green and roasted coffee.the antioxidant properties of green and roasted coffee, in relation to species (coffea arabica and coffea robusta) and degree of roasting (light, medium, dark), were investigated. these properties were evaluated by determining the reducing substances (rs) of coffee and its antioxidant activity (aa) in vitro (model system beta-carotene-linoleic acid) and ex vivo as protective activity (pa) against rat liver cell microsome lipid peroxidation measured as tba-reacting substances. rs of c. robustasam ...200010820041
ochratoxin formation in aspergillus ochraceus with particular reference to spoilage of coffee.production of ochratoxin on media by eight isolates of aspergillus ochraceus from coffee or its processing environment in india, indonesia, kenya, and brazil, and seven brazilian isolates from other commodities, has been compared with yields in shaken fermentation on shredded wheat and coffee (coffea arabica). shredded wheat most consistently allowed expression of biosynthesis of ochratoxins a and b in yields up to 3.5% of the dry product. culture on artificial media was an unreliable predictor ...200010857930
[coffea arabica--production, botanical classification, new components and active agents and their pharmacological effects].the present paper surveys the latest knowledge about coffee grains (coffea arabica) as an important part of our everyday life. it lists the botanical classification of its principal species, such as coffea arabica, coffea robusta, and coffea liberica, the most important improved cultivars of production species in the world. the review paper also discusses the economic aspect, lists the most important regions of production, the optimal and farming technological conditions. it points out the occur ...200010953453
microbial diversity during maturation and natural processing of coffee cherries of coffea arabica in brazil.the magnitude and diversity of the microbial population associated with dry (natural) processing of coffee (coffea arabica) has been assessed during a 2-year period on 15 different farms in the sul de minas region of brazil. peptone water-washed samples were taken of maturing cherries on trees (cherries, raisins and dried cherries) and from ground fermentations. the microbial load varied from 3 x 10(4) to 2.2 x 10(9) cfu/cherry with a median value of 1.6 x 10(7) cfu/cherry. the microbial load in ...200011016614
parasitoids of medfly, ceratitis capitata, and related tephritids in kenyan coffee: a predominantly koinobiont assemblage.arabica coffee was sampled from two sites in the central highlands of kenya (rurima, ruiru) and one site on the western side of the rift valley (koru). three species of ceratitidine tephritidae, ceratitis capitata (wiedemann), c. rosa karsch and trirhithrum coffeae bezzi, were reared from sites in the central highlands, and an additional species, c. anonae graham, was recovered from the western-most site. ten species of parasitic hymenoptera were reared from these tephritids. the parasitoid asse ...200011107253
novel nitrogen-fixing acetic acid bacteria, gluconacetobacter johannae sp. nov. and gluconacetobacter azotocaptans sp. nov., associated with coffee plants.diazotrophic bacteria were isolated, in two different years, from the rhizosphere and rhizoplane of coffee (coffea arabica l.) plants cultivated in mexico; they were designated as type dor and type sad isolates. they showed characteristics of the family acetobacteraceae, having some features in common with gluconacetobacter (formerly acetobacter) diazotrophicus, the only known n2-fixing species of the acetic acid bacteria, but they differed from this species with regard to several characteristic ...200111491326
an evaluation of the genetic diversity of xylella fastidiosa isolated from diseased citrus and coffee in são paulo, brazil.abstract strains of xylella fastidiosa, isolated from sweet orange trees (citrus sinensis) and coffee trees (coffea arabica) with symptoms of citrus variegated chlorosis and requeima do café, respectively, were indistinguishable based on repetitive extragenic palindromic polymerase chain reaction (pcr) and enterobacterial repetitive intergenic consensus pcr assays. these strains were also indistinguishable with a previously described pcr assay that distinguished the citrus strains from all other ...200118943950
comparison of the antioxidant activity of commonly consumed polyphenolic beverages (coffee, cocoa, and tea) prepared per cup serving.in this study, the in vitro low-density lipoprotein oxidation model was used to assess the relative antioxidant activity of the polyphenolic beverages tea, coffee, and cocoa on a cup-serving basis. the beverages were prepared as 0.7-2.5% soluble coffee and 1.5-3.5% cocoa; teas (green, black, or herbal) were prepared as one tea bag infused over 5 min in 220 ml of hot water. under these standard cup serving conditions, the antioxidant activity as determined by the lag time was in the range of 292- ...200111453788
characterization of espresso coffee aroma by static headspace gc-ms and sensory flavor profile.the aromas of three espresso coffee (ec) samples from different botanical varieties and types of roast (arabica coffee, robusta natural blend, and robusta torrefacto blend (special roast by adding sugar)) were studied by static headspace gc-ms and sensory flavor profile analysis. seventy-seven compounds were identified in all of the ec samples. among them, 13 key odorants have been quantified and correlated with their flavor notes by applying multivariate statistical methods. some correlations h ...200111714340
multivariate methods for characterization and classification of espresso coffees from different botanical varieties and types of roast by foam, taste, and mouthfeel.three espresso coffee (ec) samples of different botanical varieties and types of roast were prepared in standard conditions using an experimental ec prototype: arabica coffee, robusta natural blend, and robusta torrefacto blend (a special roast by adding sugar). the ecs were characterized with regard to the physical parameters, amount of total solids, total solids on filtrate, lipids, caffeine, trigonelline, and chlorogenic acids by hplc, and sensory descriptive analysis related to foam appearan ...200111600016
potential for areawide integrated management of mediterranean fruit fly (diptera: tephritidae) with a braconid parasitoid and a novel bait spray.the braconid wasp, fopius arisanus (sonan), a biological control agent for mediterranean fruit fly, ceratitis capitata (wiedemann), was studied in coffee, coffea arabica l. fopius arisanus, comprised 79.3% of the total parasitoids (7,014) recovered from fruits collected at three small coffee farms. data from seasonal host/parasitoid studies at a large coffee plantation also suggested that the most effective natural enemy of c. capitata in coffee may now reside in hawaii. the original parasitoids ...200111561838
trueness-to-type and agronomic characteristics of coffea arabica trees micropropagated by the embryogenic cell suspension technique.trueness-to-type and agronomic characteristics of trees of four coffee (coffea arabica l.) f(1) hybrid clones derived from embryogenic cell suspensions were compared with those of trees produced from in vitro microcuttings. three types of variants were observed among the 644 trees derived from embryogenic suspensions. total frequency of the variants was 2.1% for trees originating from embryogenic cell suspensions, whereas no variant was found among the trees produced from microcuttings. the vari ...200111560816
molecular and biochemical characterization of endo-beta-mannanases from germinating coffee (coffea arabica) grains.the activity of endo-beta-mannanase ([1-->4]-beta-mannan endohydrolase ec 3.2.1.78) is likely to be central to the metabolism of cell wall mannans during the germination of grains of coffee (coffea spp.). in the present paper, we report the cloning and sequencing of two endo-beta-mannanase cdnas (mana and manb) by different strategies from coffea arabica l.. the mana cdna was obtained by the use of oligonucleotides homologous to published sequences of other endo-beta-mannanases and manb by the u ...200111469596
origin, diversity and evolution of nbs-type disease-resistance gene homologues in coffee trees (coffea l.).the majority of plant disease-resistance genes (r-genes) isolated so far encode a predicted nucleotide-binding site (nbs) domain. nbs domains related to r-genes show a highly conserved backbone of amino acid motifs, which makes it possible to isolate resistance gene analogues (rgas) by pcr with degenerate primers. multiple combinations of primers with low degeneracy, designed from two conserved motifs in the nbs regions of r-genes of various plants, were used on genomic dna from coffee trees, an ...200111459185
chlorogenic acids as a potential criterion in coffee genotype selections.a systematic study by hplc was conducted to determine the content of chlorogenic acids in green coffee beans of the variedad colombia (coffea arabica caturra var. x híbrido de timor) and in other genotypes of interest of c. arabica, coffea canephora, híbrido de timor, and the f1 offspring derived from the crossing caturra x híbrido de timor. comparisons were made of the total content of these acids, their quantitative differences, and the presence or absence of some of them, using parametric sta ...200111368619
optimizing headspace temperature and time sampling for identification of volatile compounds in ground roasted arabica coffee.equilibration time and temperature were the factors studied to choose the best conditions for analyzing volatiles in roasted ground arabica coffee by a static headspace sampling extraction method. three temperatures of equilibration were studied: 60, 80, and 90 degrees c. a larger quantity of volatile compounds was extracted at 90 degrees c than at 80 or 60 degrees c, although the same qualitative profile was found for each. the extraction of the volatile compounds was studied at seven different ...200111312865
chemical characterization of the high molecular weight material extracted with hot water from green and roasted arabica coffee.the polysaccharides present in coffee infusions are known to contribute to the organoleptic characteristics of the drink, such as the creamy sensation perceived in the mouth known as "body", the release of aroma substances, and the stability of espresso coffee foam. to increase the knowledge about the origin, composition, and structure of the polysaccharide fraction, the high molecular weight material (hmwm) was extracted with hot water from two green and roasted ground arabica coffees: costa ri ...200111308325
polysaccharides of green arabica and robusta coffee beans.two independent procedures for the quantitative determination of the polysaccharide content of arabica caturra (coffea arabica var. caturra) and robusta rom (coffea canephora var. rom) green coffee beans showed that they both contained identical amounts of polysaccharide. cell wall material (cwm) was prepared from the beans and partial solubilisation of component polysaccharides was effected by sequential extraction with water, 1 m koh, 0.3% naclo2, 4 m koh and 8 m koh. the monosaccharide compos ...200111217967
introgression into the allotetraploid coffee ( coffea arabica l.): segregation and recombination of the c. canephora genome in the tetraploid interspecific hybrid ( c. arabicax c. canephora).transfer of desired characters from the diploid relative species such as coffea canephora into the cultivated allotetraploid coffee species ( coffea arabica l.) is essential to the continued improvement of varieties. behaviour of the c. canephora genome and its interaction with the c. arabica genome were investigated in tetraploid interspecific hybrids ( c. arabicax c. canephora 4 x) resulting from a cross between an accession of c. arabica and a tetraploid plant of c. canephora obtained followi ...200212582671
the origin of cultivated coffea arabica l. varieties revealed by aflp and ssr markers.molecular markers were used to assess polymorphism between and within the genetic bases of coffee (i.e. typica and bourbon) spread from yemen since the early 18th century that have given rise to most arabica cultivars grown world-wide. eleven coffea arabica accessions derived from the disseminated bases were evaluated by amplified fragment length polymorphism (aflp) using 37 primer combinations and simple-sequence repeats (ssrs) produced by six microsatellites. four cultivars growing in yemen an ...200212582651
aflp analysis of genetic diversity within and among coffea arabica cultivars.genetic diversity of coffea arabica cultivars was estimated using amplified fragment length polymorphism (aflp) markers. sixty one coffea accessions composed of six arabica cultivars, including typica, bourbon, catimor, catuai, caturra and mokka hybrid, plus two diploid coffea species, were analyzed with six ecori- msei primer combinations. a total of 274 informative aflp markers were generated and scored as binary data. these data were analyzed using cluster methods in the software package ntsy ...200212582521
gene introgression into coffea arabica by way of triploid hybrids (c. arabica x c. canephora).interspecific triploid hybrid plants between the tetraploid species coffea arabica l. and the diploid species c. canephora p. were backcrossed to c. arabica. although characterised by a low production and an important fruit dropping, all attempted crosses (ie, 6) generated bc(1) progenies. flow cytometric analysis of the nuclear dna content revealed that most of the bc1 individuals were nearly tetraploid. among the male gametes produced by the interspecific triploid hybrids, those presenting a h ...200212466993
origin assessment of green coffee (coffea arabica) by multi-element stable isotope analysis of caffeine.the delta(13)c(vpdb), delta(2)h(vsmow) and delta(18)o(vsmow) values of caffeine isolated from arabica green coffee beans of different geographical origin have been determined by isotope ratio mass spectrometry (irms) using elemental analysis (ea) in the "combustion" (c) and "pyrolysis" (p) modes (ea-c/p-irms). in total, 45 coffee samples (20 from central and south america, 16 from africa, six from indonesia, and three from jamaica and hawaii) were analysed, as well as three reference samples of ...200212434246
[bacterial present in the xylem of coffee (rubiaceae: coffea arabica) with "crespera" disease]."crespera" is an infectious disease of coffee plants that affects both the coffee production and the economy of the coffee producer countries. this disease affects morphologically the plant: long and narrow leaves with wavy borders and marginal necrosis; strong chlorosis results in drying of the leave, and leads to bad conditions of the plant. the internodes are short, producing the appearance of multiple sprouts in the axial sprout, the flowers can turn greenish, and the plant can present branc ...200212298265
beneficial effect of post -thawing osmoconditioning on the recovery of cryopreserved coffee (coffea arabica l.) seeds.osmoconditioning-controlled rehydration of seeds in a solution with low osmotic potential -has been shown to reinvigorate aged seeds. the present work aimed at investigating the effect of osmoconditioning on the germination of cryopreserved seeds of coffea arabica, whose viability and vigour are drastically affected by cryopreservation. for cryopreservation, seeds were desiccated to 0.21 g h2o/g dw, cooled at 1 degree c/min to -50 degree c, then immersed rapidly in liquid nitrogen. after rapid r ...200212148064
on the role of (-)-2-methylisoborneol for the aroma of robusta coffee.the role of 2-methylisoborneol (mib) in coffee aroma is controversially discussed in the literature. mib is known as an off-flavor compound in drinking water and food, but it has also been suggested as a key flavor component of robusta coffee, discriminating robusta from arabica coffee. to check this hypothesis the role of mib in coffee brews was studied. two reference samples containing pure arabica and robusta coffee brews were compared with five samples of arabica coffee brews containing incr ...200212137492
comparison of somatic embryogenesis-derived coffee (coffea arabica l.) plantlets regenerated in vitro or ex vitro: morphological, mineral and water characteristics.coffea arabica l. plantlets obtained ex vitro after sowing somatic embryos produced in a bioreactor in horticultural substrate were compared with those obtained in vitro from the same embryo population under conventional culturing conditions on semi-solid media. the intensity and quality of aerial and root system development were compared. shoot emergence was more efficient in vitro but rooting frequencies were low. in contrast, all ex vitro-regenerated embryos rooted. the cotyledon area of matu ...200212125775
the value of bees to the coffee harvest.the self-pollinating african shrub coffea arabica, a pillar of tropical agriculture, was considered to gain nothing from insect pollinators. but i show here that naturalized, non-native honeybees can augment pollination and boost crop yields by over 50%. these findings, together with world coffee-harvest statistics and results from field studies of organically shade-grown coffee, indicate that coffee plants would benefit from being grown in habitats that are suitable for sustaining valuable poll ...200212066176
effect of roasting on the antioxidant activity of coffee brews.colombian arabica coffee beans were roasted to give light, medium, and dark samples. their aqueous extracts were analyzed by gel filtration chromatography, uv-visible spectrophotometry, capillary electrophoresis, and the abts(*)(+) assay. a progressive decrease in antioxidant activity (associated mainly with chlorogenic acids in the green beans) with degree of roasting was observed with the simultaneous generation of high (hmm) and low molecular mass (lmm) compounds possessing antioxidant activi ...200212059145
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