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screening of uruguayan plants for deterrent activity against insects.we evaluated the anti-insectan activity of extracts from different vegetative parts of ten plant species native to uruguay. the selected plants belong to five families: bignoniaceae: clytostoma callistegioides, dolichandra cynanchoides, macfadyena unguis-cati; sapindaceae: dodonaea viscosa, allophylus edulis, serjania meridionalis; lamiaceae: salvia procurrens, salvia guaranitica; solanaceae: lycium cestroides; and phytolaccaceae: phytolacca dioica. the extracts were evaluated in independent bio ...020046902
barley transcriptome analyses upon interaction with different aphid species identifies thionins contributing to resistance.aphids are phloem-feeding insects that cause yield loss on a wide range of crops, including cereals such as barley. while most aphid species are limited to one or few host species, some are able to reproduce on many plants belonging to different families. interestingly, aphid probing-behaviour can be observed on both host and non-host species indicating that interactions take place at the molecular level that may impact host range. here, we aimed to gain insight into the interaction of barley wi ...028452058
metabolite profiling reveals a specific response in tomato to predaceous chrysoperla carnea larvae and herbivore(s)-predator interactions with the generalist pests tetranychus urticae and myzus persicae.the spider mite tetranychus urticae koch and the aphid myzus persicae (sulzer) both infest a number of economically significant crops, including tomato (solanum lycopersicum). although used for decades to control pests, the impact of green lacewing larvae chrysoperla carnea (stephens) on plant biochemistry was not investigated. here, we used profiling methods and targeted analyses to explore the impact of the predator and herbivore(s)-predator interactions on tomato biochemistry. each pest and p ...027610113
the green peach aphid myzus persicae perform better on pre-infested chinese cabbage brassica pekinensis by enhancing host plant nutritional quality.the green peach aphid, myzus persicae sulzer, is a notorious pest on vegetables, which often aggregates in high densities on crop leaves. in this study, we investigated whether m. persicae could suppress the resistance level of chinese cabbage brassica pekinensis. m. persicae performed better in terms of weight gain (~33% increase) and population growth (~110% increase) when feeding on previously infested (pre-infested) chinese cabbage compared with those on non-infested plants. however, when gi ...026905564
parathion for control of green peach aphid on shade-grown tobacco. 194718935286
factors affecting the activity of alatae of the aphids myzus persicae (sulzer) and brevicoryne brassicae (l.). 194918116923
over-wintering of aphids, especially myzus persicae, sulzer, in root clamps. 194915402983
the grouping and overwintering of myzus persicae sulz. on prunus species. 194918143239
the green peach aphid on tobacco in the dominican republic. 195213028257
feeding behaviour of the aphids myzus persicae and brevicoryne brassicae, studied with radiophosphorus. 195313051326
evidence that potato virus y is carried near the tip of the stylets of the aphid vector myzus persicae (sulz.). 195513270154
does the aphid myzus persicae (sulz.) imbibe tobacco mosaic virus? 195613325405
three more viruses borne at the stylet tips of the aphid myzus persicae (sulz.). 195713437229
studies on the behavior of potato leaf roll and other viruses in the body of their aphid vector myzus persicae (sulz.). 195813581530
[on the functional morphology of the salivary glands of homoptera. part i. the chief cells of the principal gland of myzus persicae (sulz.), aphididae]. 196014423192
[on functional morphology of salivary glands of homoptera. report 3: the accessory gland of myzus persicae (sulz.), aphididae]. 196013786197
[on the functional morphology of the salivary glands of homoptera. iv. the secretory ducts of the salivary glands of myzus persicae (sulz.), aphididae]. 196013771645
re-evaluation of retention of the beet yellows virus by green peach aphids under fasting and feeding conditions. 196113774366
short-term probing behavior of myzus persicae (sulzer) as affected by fasting, anesthesia, and labial manipulation. 196313979776
fatty acids: in vivo synthesis by the green peach aphid, myzus persicae (sulzer).after feeding through an artificial membrane on an 18 percent sucrose solution containing either acetate-1-c(14) or uniformly-labeled glucose-c(14), myzus persicae incorporated 75 percent of the carbon-14 into palmitoleic, stearic, and oleic acids. small amounts were incorporated into myristic, linoleic, and linolenic acids; no significant amounts were incorporated into the short-chain fatty acids.196317836485
studies on the artificial feeding of the aphid myzus persicae (sulzer)--3. some major nutritional requirements. 19655827534
the purification of viruslike particles from the aphid myzus persicae. 196514295230
[some relations between the common pea mosaic virus and the peach tree aphis myzus persicae sulz]. 19655856645
endosulfan, oxydemetonmethyl, and endrin in control of the green peach aphid and suppression of leaf roll in potatoes in eastern washington. 19665976106
improvement of synthetic diet for the aphid myzus persicae using plant juices, nucleic acids, or trace metals. 19676046968
[effect of some factors on the development of alate forms in the peach louse (myzus persicae sulz.)]. 19675586737
the purification of potato leafroll virus from its vector myzus persicae. 19676017406
application of scanning microscopy in the study of virus transmission of aphids.the scanning electron microscope was used in conjunction with the transmission electron microscope to study stylet morphology and the role of stylets of the green peach aphid in plant virus transmission. the morphology of the stylets generally agreed with earlier descriptions of these parts obtained with the transmission electron microscopy. a considerable variation seemed to exist in the extent and regularity of ridges observed in different individuals, especially among apterous adults and nymp ...196816789086
aphid transmission of tobacco mosaic virus.aphids (myzus persicae sulz.) can acquire tobacco mosaic virus from tobacco leaves coated with a virus suspension and inoculate it into healthy leaves. transmission depends on virus concentration, period of acquisition, previous feeding history of the aphids, and time between acquisition and transmission feedings. aphids whose stylets are cut do not transmit the virus.19695783713
suspected insecticide resistance mechanism in the peach-potato aphid. 197016056843
comparison of the biology of myzus persicae sulz. resistant and susceptible to dimethoate. 19705491865
effect of artificial diet composition on wing-production by the aphid myzus persicae. 19705449726
dietary amino acid requirements of the aphid myzus persicae affected by antibiotic uptake. 19715565091
variation in the photoperiodic response within natural populations of myzus persicae (sulz.). 197122894860
mass rearing parasite-free green peach aphids in the greenhouse. 19715558295
chromosomal abnormalities in an anholocyclic biotype of myzus persicae (sulzer). 19715556464
 certain plants are able to convert first stage larvae of the green peach aphid into apterous larvae although the larger part of them would develop into alates if reared continuously on a holidic diet.this apterousness promoting principle (app principle) has a greater effect on previously starved larvae and on those whose extremities were partly amputated. the app principle can be shown most clearly in young radish seedlings, as well as in other young plants if they are optimal hosts. it is reduc ...197128312448
the fine structure of the mycetome symbiotes of the aphids brevicoryne brassicae, myzus persicae, and macrosiphum rosae. 19714999588
effects on myzus persicae (sulz.) and transmission of beet yellows virus of applying certain trace elements to sugar beet. 19715133524
evaluation of systemic insecticides for green peach aphid control on tobacco. 197120333851
transmission of tobacco mosaic virus by myzus persicae. 19725084452
[effect of sinigrin on sucrose uptake by some polyphagous and oligophagous aphids (aphididae)].the effect of 0.1% sinigrin on sucrose uptake (20% aqueous solution) by some polyphagous and oligophagous aphid species was tested using the tracer method. in the presence of sinigrin the sucrose ingestion of brevicoryne brassicae, myzus persicae and megoura viciae was promoted. with rhopalosiphum padi there was a strong, and with aphis fabae and acyrthosiphon pisum only a slight inhibition of sucrose uptake. aulacorthum circumflexum reacted positively in some experiments, but negatively in othe ...197228313041
insect predators for controlling aphids on potatoes. 8. green peach aphid consumption by coccinella septempunctata and c. transversoguttata. 19725085802
control of green peach aphid on sweet peppers in delaware. 19724628649
an insecticide and a defoliant evaluated for use in a program of integrated control designed to suppress the green peach aphid on peach trees. 19725008977
an in vitro study of esterases hydrolysing specific substrates of an op-susceptible and an op-resistant strain of the green peach aphid, myzus persicae sulz. 19734788463
an electrophoretic study of some hydrolases from an op-susceptible and an op-resistant strain of the green peach aphid, myzus persicae (sulz.). 19734350965
control of the green peach aphid and suppression of leaf roll on potatoes by systemic soil insecticides and multiple foliar sprays. 19734690263
incorporation of thymidine into the chromosomes of aphid (myzus persicae) embryos. 19744474087
sugar, organic acid, phenolic acid and plant growth regulator content of extracts of honeydew of the aphid myzus persicae and of its host plant, raphanus sativus.the honeydew of myzus persicae feeding on radish seedlings contains glucose, fructose, trehalose, melezitose and sucrose, of which glucose and fructose are present in radish seedlings. in addition, the honeydew contains eight organic acids, of which seven are present in uninfested seedlings and six are present in infested radish seedlings: of the eighteen phenolic acids in the honeydew, five are present in uninfested radish seedlings. auxins, gibberellins, growth inhibitors and cytokinins are pr ...197419280790
[effect of atp in a synthetic diet on myzus persicae (sulz.) (aphidina)]. 19744847667
[the suitability of megoura viciae(buckt.) (aphid.) for larvae and adult females of aphelinus asychis walker (aphelin.)].in the laboratory, the females of the polyphagous aphid parasite aphelinus asychis accepted vetch aphids (megoura viciae on vicia faba) for oviposition and host feeding as readily as peach aphids (myzus persicae on brassica oleracea var. gongylodes) of the same size. however, the vetch aphids defended themselves less often; consequently, they were parasitized more frequently.with very few exceptions, all parasite larvae in the vetch aphids died after some days. blood reactions were scarcely noti ...197428308698
intraspecific variation of myzus persicae on sugar beet (beta vulgaris).differences in inherited resistance among seven sugar-beet stocks had similar effects on myzus persicae clones representing the range of variation in aphid response to resistant and susceptible sugar beet observed in fifty-eight clones collected between 1969 and 1971. three sugar-beet stocks were consistently resistant. statistically significant interactions between beet stocks and aphid clones did not indicate the existence of biotypes with specific abilities to overcome resistance. m. persicae ...197419280785
[investigations of beet mild yellowing. 1. virus transmission by myzus persicae sulz. after feeding of plant extracts through an artificial membrane (author's transl)]. 19744480507
[the effect of salivary gland (obtained from myzus persicae sulzer) extract on broad bean epidermal cells infected with bean yellow mosaic virus (author's transl)]. 19744478240
[nutritional budgets for third stage instars of myzus persicae (sulz.)].a general equation is provided for the nutritional budgets of both morphal stages of third-instar larva of the green-peach aphid myzus persicae (sulz.), fed on an artificial diet. uptake of food and oxygen is related to: 1) gain in (fresh) weight, 2) amount of excrement, 3) carbon dioxide respired, and 4) water lost by transpiration (table 4).aphids fed holidicly exhibit a respiratory quotient of 1.0 during food uptake, thus using mainly carbohydrates during respiration. hence, based upon quanti ...197528308812
[rearing of a biotype of myzus persicae sulzer on synthetic media. iii. respective nutritive value of l-methionine, l-cysteine and sulfates].the respective influence of some different components of a synthetic diet on the survivial and the fecundity of one strain of myzus persicae slz. have been established by experiments with the deletion of some components and with variations of their concentrations. 1. l.methionin is the only essential amino-acid amd the variations of its concentrations are related with variations of the fecondity of the aphids. 2. when the amount of l. methionin is inadequate to satisfy the whole sulphur requirem ...19751227369
role of monoamine metabolism in wing dimorphism of the aphid myzus persicae. 197611073
simultaneous transmission of broad bean wilt and cucumber mosaic viruses by single myzus persicae (sulz.). 1976947110
[laboratory and field tests about the transmission of erysimum latent virus by phyllotreata species (chrsomelidae) (author's transl)].erysimum latent virus (elv) proved to be transmissible by phyllotreta atra, p. nemorum, p. nigripes, and p. undulata. from them p. nemorum is a new discovered vector. the acquisition of the virus by the beetles was possible within a feeding time of less than 10 minutes. because virus transmission could be performed within 30 to 60 minutes immediately after virus uptake there exists apparently no latent period in the vector. the retention or persistence of elv in the phyllotreta species amounted ...1977565119
the properties of a carboxylesterase from the peach-potato aphid, myzus persicae (sulz.), and its role in conferring insecticide resistance.carboxylesterases from different strains of myzus persicae were examined to try to understand their contribution to insecticide resistance. preliminary evidence that they are involved comes from the good correlation between the degree of resistance and the carboxylesterase and paraoxon-degrading activity in aphid homogenates. furthermore the carboxylesterase associated with resistance could not be separated from the insecticide-degrading enzyme by electrophoresis or ion-exchange chromatography. ...1977603629
[a conbribution to the characterization of asparagus virus 2 (author's transl)].in host range investigations with 152 plant species of 37 families, 96 species from 28 families proved to be experimentally susceptible to asparagus virus 2 (asv 2). from these, 82 species of 26 families were systematically infectible and 14 species of 10 families only locally. most frequently were symptomless systemic infections. of 86 species, their susceptibility for asv 2 was unknown before. the number of families that contant experimental hosts of the virus increased from 11 to 33. transmis ...1978664937
electrophoretic study of genetic variation in british myzus persicae (sulz.) (hemiptera, aphididae). 1978743199
[breeding of a biotype of the aphia myzus persicae on a synthetic medium. iv. influence of vitamins, especially vitamin c and pyridoxine, on the nutritional value of nutritive fluids].the nutritive value of synthetic diets for myzus persicae was evaluated by measuring reproduction of the third generation on each medium: 1. in the presence of a double dose of pyridoxin, the nutritional value of the basic diet was improved. this improvement disappeared when all the vitamins of the b group were also at double dose; 2. in the presence of a double dose of ascorbic acid, the nutritional value of all the diets was improved. this improvement was observed for a single or a double dose ...1978104648
acetaldehyde--a potential fumigant for control of the green peach aphid on harvested head lettuce. 1979528743
[breeding of a biotype of myzus persicae sulzer on a synthetic medium. v- influence of oxalic and gentisic acids on the nutritive value of a synthetic medium].studies on the survival, fecundity, and food consumption by m. persicae reared on synthetic diets, have indicated: --gentisic and oxalic acids have a deleterious effect as most important as their dose is high. --succinic acid reduces, at least for the used concentrations, the deleterious effect of the oxalic acid, but not that of the gentisic acid. --gentisic acid is quickly decreasing food consumption. --the deleterious effect of oxalic and gentisic acids is not decreasing with time. the result ...19807416660
toxicity of [14c] phorate applied systemically to the aphids myzus persicae (sulz) and brevicoryne brassicae (l.) grown on brussels sprout plants.the effect of foliar application of [14c] phorate on the larvae of m. persicae and b. brassicae was studied. regression analysis of the toxic components in the foliage and the probit kill showed that b. brassicae was more susceptible to the insecticide than m. persicae. the level of radioactivity extracted from the leaves, aphids and their honeydew shows that there was no correlation between the applied dose and the mortality. the amounts of honeydew produced by colonies before and after applica ...19806159846
feeding behaviour and susceptibility of myzus persicae (sulz) and brevicoryne brassicae (l.) to [14c] phorate in artificial diet.the effect of artificial diet on the growth and reproduction of the aphids myzus persicae (sulz) and brevicoryne brassicae (l) was studied. following the application of [14c] phorate to the diet, the results showed that b. brassicae was more susceptible than m. persicae. in these experiments the dead and live aphids and their honeydew were also analysed. the dead aphids contained more toxic 14c than the live ones with a direct correlation between the amount of insecticide in the diet and the ins ...19806155883
different helper factors associated with aphid transmission of some potyviruses.myzus persicae transmitted watermelon mosaic virus (wmv) after acquiring it through artificial membranes from a solution of purified virus mixed with the soluble fraction from infected leaf extracts or by prefeeding on the soluble fraction before acquiring purified virus. wmv-induced helper factor assisted m. persicae in transmitting purified turnip mosaic virus (tumv) but not potato virus y (pvy). tumv-induced helper factor from infected leaves was ineffective for the transmission of purified w ...198118635077
partitioning yield loss on yellow squash into nematode and insect components.the effect of a contplex of several insect and nematode pests on yield of yellow squash (cucurbita pepo l.) was examined in two field tests in southern florida. applications of permethrin for insect control and oxamyl primarily for nematode control plus some insect control were made alone and in combination to achieve differential reduction of various insect and nematode components contributing to yield loss. the effect of these components on yield was further analyzed by multiple regression. yi ...198219295683
disappearance of dimethoate, methamidophos and pirimicarb in lettuce.foliar sprays of dimethoate at 150 or 300 g a.i./ha, methamidophos at 450 or 900 g a.i./ha and pirimicarb at 140 or 280 g a.i./ha were applied for control of the green peach aphid, myzus pericae (sulzer), and the lettuce aphid, nasonovia ribisnigri ( mosley ), about 2 weeks before the lettuce started heading, and again about 1 week from harvest. in lettuce, dimethoate partially oxidized to its oxon and pirimicarb converted to its methylamino- and/or formyl methylamino-analogues. most residues we ...19846736564
maximizing the detection capability of a beet western yellows virus elisa system.conditions for maximizing detection of a california isolate of beet western yellows virus (bwyv) were investigated with the double-sandwich enzyme-linked immunosorbent assay (elisa) system. within-plate variability was found to account for less than 1% of the total variation observed on individual microtiter plates. variability across plates was greater than within plates and accounted for less than 10% of the total variation. significant reductions in absorbance were observed when either coatin ...19846392314
the effect of helper component on the uptake and localization of potyviruses in myzus persicae.125i-labeled virions were used to determine whether helper component (hc) affected the uptake or distribution of potyviruses in aphids. aphids were allowed to acquire purified, 125i-labeled tobacco etch virus or potato virus y mixed with hc or with inactivated hc. helper component had no effect upon uptake of labeled virus, as measured by gamma counting. autoradiography of freeze-sectioned aphids revealed, however, that in the presence of hc, label was associated with the maxillary stylets and w ...198618640647
overproduction of detoxifying esterases in organophosphate-resistant culex mosquitoes and their presence in other insects.antisera raised against the denatured polypeptide of two organophosphate-detoxifying esterases (b1 and a1) of culex mosquitoes were used in an immunoblot method to quantify esterase production in resistant versus susceptible strains and to detect the presence of immunologically related proteins in other insects. it was demonstrated that esterase b1 of culex quinquefasciatus and esterase a1 of culex pipiens are overproduced in resistant strains by factors of at least 500-fold and 70-fold, respect ...19873470782
a multiple homogenizer for rapid sample preparation in immunoassays and electrophoresis.a multiple homogenizer is described for preparing samples of small invertebrates or tissue in a flat-bottom immunoplate. its efficiency was evaluated by immunoassay of carboxylesterase (e4), the enzyme conferring insecticide resistance in the peach potato aphid (myzus persicae). this equipment was shown to release more enzyme, with less variability, than homogenizing individual aphids and its efficiency allows one person to analyze up to 3000 individual insects per day. it is also suitable for p ...19873447586
differential inhibition by castanospermine of various insect disaccharidases.the indolizidine alkaloid, castanospermine (1,6,7,8-tetrahydroxy-octahydroindolizidine-a stereochemical mimic of glucose found in the australian legumecastanospermum australe), differentially inhibited cellobiose, lactose, maltose, sucrose, and trehalose hydrolyzing enzymes from a broad taxonomic spectrum of insects (19 species from 12 different families). it was a potent inhibitor of cellobiase activity of all insects tested (50% inhibition at <3.2 × 10(-5) m castanospermine). with one exceptio ...198724302343
[salivary glands of myzus persicae (homoptera, aphididae): structure and secretory rhythm].the salivary glands of myzus persicae (homoptera, aphididae): structure and secretory rythm. studying the structure of the salivary glands of myzus persicae, a particular mode of working of these organs were observed. the glands seem to function in an alternative manner. this aspect is discussed with regards to the nutritional biting of the bug.19873442441
preference of the green peach aphid, myzus persicae, for plants grown in sewage sludges. 19873663979
molecular evidence that insecticide resistance in peach-potato aphids (myzus persicae sulz.) results from amplification of an esterase gene.cdna clones for the esterase (e4) responsible for broad insecticide resistance in peach-potato aphids (myzus persicae sulz.) were isolated and used to study the molecular basis of resistance. increased esterase synthesis by resistant aphids was found to be associated with amplification of the structural gene for the esterase (e4 or its closely related variant, fe4), the degree of amplification being correlated with the activity of the esterase and the level of resistance. hybridization of the cd ...19883390158
transfer of resistance to plrv titer buildup from solanum etuberosum to a tuber-bearing solanum gene pool.hybrids between solanum etuberosum and s. pinnatisectum harboring resistance to titer buildup of potato leafroll virus (plrv) were reciprocally crossed with tuber-bearing wild species s. acaule and s. verrucosum. a total of 47 hybrids with acaule were obtained with the aid of embryo rescue and sterile culturing of embryos from imbibing seeds. all but two hybrids with acaule had low pollen stainabilities or were pollen sterile. hybrid seeds from crosses with verrucosum were easily obtained, and t ...198824231994
coat protein of potyviruses. 4. comparison of biological properties, serological relationships, and coat protein amino acid sequences of four strains of potato virus y.four strains of potato virus y, pvy-d, pvy-10, pvy-18, and pvy-43, obtained from different australian sources were compared on the basis of their biological, serological and coat protein structural properties. each of the strains could be distinguished on the basis of their reactions on selected test plant species. two of the pvy strains, pvy-d and pvy-10, induced symptoms similar to those produced by the pvyo strain group. the reactions of pvy-18 and pvy-43, although comparable to pvyn in some ...19883144263
[a comparative study of the effect of vinyl phosphoric acid esters on cholinesterase and carboxylesterase activities in mammals and arthropods].studies have been made of the effect of organophosphorus inhibitors on cholinesterase and carboxylesterase from various mammals (human erythrocytes, mouse brain, blood serum of mouse and rat, blood serum of horse) and arthropods (calliphora vicina, schizaphis graminum, myzus persicae, sitophilus oryzae, pseudococcus maritimus, tetranychus urticae). organophosphorus inhibitors were presented by esters of vynylphosphoric acid containing normal and branched alkyls in the phosphoryl part of the mole ...19902360379
engineering resistance to mixed virus infection in a commercial potato cultivar: resistance to potato virus x and potato virus y in transgenic russet burbank.potato virus x (pvx) and potato virus y (pvy) infection in potato may result in the loss of certification of seed potatoes and affect quality and yield of potatoes in commercial production. we transformed a major commercial cultivar of potato, russet burbank, with the coat protein genes of pvx and pvy. transgenic plants that expressed both cp genes were resistant to infection by pvx and pvy by mechanical inoculation. one line was also resistant when pvy was inoculated with viruliferous green pea ...19901366358
honeydew analysis for detecting phloem transport of plant natural products : implications for host-plant resistance to sap-sucking insects.analysis of honeydew excreted by various sap-sucking insects indicated the presence of certain plant secondary constituents in the phloem of their host plants. honeydew excreted by mealybugs (pseudococcus longispinus), living oncastanospermum australe, contained the indolizidine alkaloid castanospermine, a potent β-glucosidase inhibitor. similarly, honeydew from green peach aphids (myzus persicae), feeding onsenecio vulgaris flower buds, contained the pyrrolizidine alkaloid senecionine, itsn-oxi ...199024263993
sucrose esters of carboxylic acids in glandular trichomes ofsolanum berthaultii deter settling and probing by green peach aphid.removal of type b trichome exudate fromsolanum berthaultii leaflets leads to a decrease in tarsal gumming and mortality and an increase in feeding by the green peach aphid,myzus persicae. type b trichome exudate of thes. berthaultii accession pi 473331 is composed of a complex of 3',3,4,6-tetra-o-acyl sucroses containing primarily short-chain branched carboxylic acids. the acyl constituents are primarily derived from 2-methylpropanoic, 2-methylbutyric, and 8-methylnonanoic acids but constituents ...199024263505
bird-repellent properties of secretions from nymphs of the azalea lace bug.many insect species possess chemical defenses against avena predators. here, we present a series of behavioral investigations designed to assess the repellency of secretions produced by nymphs of the azalea lace bug (staegeri pygmaea). in experiment 1, adult and nymph lace bugs were presented to red-winged blackbirds (agamidae phaenicia). the results indicated that adults (which lack chemical secretions) were relatively more palatable. in experiment 2, we dipped nymphs in ethylene chloride to re ...199127755667
visualization of a luteovirus in the vector aphid's body by two gold immunolabelling techniques: a comparative study.two gold immunolabelling techniques using electron microscopy were compared to examine the in situ localization of a luteovirus, potato leafroll virus (plrv), inside its main aphid vector, myzus persicae sulz. with gildow's technique, virus particles were labelled prior to fixation, embedding by injecting plrv-specific iggs into the living aphids. this facilitated the detection of extracellular particles located between the basal lamina and plasmalemma by trapping them in aggregates. the heavy c ...19911800527
resistance to phloem transport of potato leafroll virus in potato plants.a 'double-graft sandwich' technique in which sections of potato stem from different potato cultivars were grafted between a susceptible healthy stock plant and a potato leafroll virus (plrv)-infected scion was used to study the rate of phloem transport of plrv in cultivars differing in resistance to plrv infection (ir) and accumulation (ar). resistance to phloem transport (i.e. delayed plrv systemic movement) was found in bismark cultivar (ir a(s)). this was independent of ir and ar as the rate ...19921469360
insect-mediated transmission of mixed and reassorted cucumovirus genomic rnas.transmissions of virus using the aphid myzus persicae were performed using plants co-infected with two cucumoviruses, tomato aspermy virus (v-tav) and cucumber mosaic virus (m-cmv). five of the aphid-transmitted progeny viruses (3.7%) induced symptoms distinct from those induced by either parental virus. northern blot hybridization analysis of encapsidated rnas from these novel progeny demonstrated that all of the rna profiles were characteristic of pseudo-recombinants, i.e. viruses with reassor ...19921645147
cytokinin-mediated insect resistance in nicotiana plants transformed with the ipt gene.the bacterial isopentenyl transferase (ipt) gene involved in cytokinin biosynthesis was fused with a promoter from the proteinase inhibitor ii (pi-iik) gene and introduced into nicotiana plumbaginifolia. transcripts of the ipt gene were wound-inducible in leaves of transgenic pi-ii-ipt plants. in leaf disks excised from fully expanded leaves, transcript levels increased 25- to 35-fold within 24 h and by 48 h were reduced by about 50%. in flowering plants, message levels were 2- to 5-fold higher ...19938219068
signal for potyvirus-dependent aphid transmission of potato aucuba mosaic virus and the effect of its transfer to potato virus x.a british isolate of potato aucuba mosaic potexvirus (pamv) was transmitted by aphids (myzus persicae) which had fed previously on a source of potato y potyvirus (pvy). nucleotide sequence analysis of the pamv coat protein gene indicated that amino acid residues 14 to 16 from the n terminus of the coat protein have the sequence dag, which is also found in the coat proteins of potyviruses and is required for their aphid transmissibility. a recombinant virus isolate (txpa7) was produced in which a ...19938336116
the intestine is a site of passage for potato leafroll virus from the gut lumen into the haemocoel in the aphid vector, myzus persicae sulz.four detection techniques, three of which gave reliable identification of the virus particles, were used to locate potato leafroll virus (plrv) in the alimentary canal of its main aphid vector, myzus persicae sulz: immunofluorescence on cryostat sections, conventional transmission electron microscopy on ultrathin sections and immune electron microscopy with gold labeling, either prior to or after fixation-embedding. each method clearly showed the presence of the virus in the intestine epithelium ...19938347080
cloning and analysis of the esterase genes conferring insecticide resistance in the peach-potato aphid, myzus persicae (sulzer).full-length cdna clones encoding the esterases (e4 and fe4) that confer insecticide resistance in the peach-potato aphid [myzus persicae (sulzer)] were isolated and characterized. the e4 cdna contained an open reading frame of 1656 nucleotides, coding for a protein of 552 amino acids. the fe4 cdna shared 99% identity with e4 over this region, the most important difference being a single nucleotide substitution resulting in the fe4 mrna having an extra 36 nucleotides at the 3' end. the derived am ...19938373371
changes in the amino acid sequence of the coat protein readthrough domain of potato leafroll luteovirus affect the formation of an epitope and aphid transmission.potato leafroll luteovirus (plrv) is transmitted naturally by aphids, but two isolates (15 and v) are known to be only poorly transmissible (pat); these isolates are also distinct in that their particles lack an epitope present in transmissible (hat) isolates of plrv (tamada et al., ann. appl. biol. 104, 107-116, 1984; massalski and harrison, j. gen. virol. 68, 1813-1821, 1987). virus cultures propagated vegetatively for several years in potato plants from the source of isolate v were shown stil ...19947513925
the peach-potato aphid myzus persicae and the tobacco aphid myzus nicotianae have the same esterase-based mechanisms of insecticide resistance.biochemical and molecular studies have established that in the peach-potato aphid, myzus persicae, insecticide resistance is conferred by amplification of genes encoding the insecticide-detoxifying esterases e4 or fe4. here we report that two insecticide-resistant clones of the closely related tobacco aphid myzus nicotianae have elevated esterases indistinguishable from e4 and fe4 and amplified esterase dna sequences, and flanking regions, with identical restriction maps to the m. persicae genes ...19947894746
endosymbiotic bacteria associated with circulative transmission of potato leafroll virus by myzus persicae.in order to understand the molecular mechanisms underlying circulative transmission of potato leafroll virus (plrv) by aphids, we screened myzus persicae proteins as putative plrv binding molecules using a virus overlay assay of protein blots. in this way, we found that purified plrv particles exhibited affinity for five aphid proteins. the one most readily detected has an m(r) of 63k, and was identified as symbionin. this is the predominant protein synthesized by the bacterial endosymbiont of t ...19947931143
transmission by aphids of a naturally non-transmissible plum pox virus isolate with the aid of potato virus y helper component.two spanish plum pox virus (ppv) isolates, 5.15 and 3.3, were used in transmission experiments involving the aphid vector myzus persicae, with woody and herbaceous host plants. these isolates differ in the size of their coat protein (cp) and sequence analysis revealed that isolate 3.3 has a 15 amino acid deletion near the n terminus of the cp, affecting the same positions as in a previously reported non-aphid-transmissible ppv isolate from germany. aphid transmission experiments showed that isol ...19957561767
[a preliminary study on bioactivity of orange and tangerine peel extracts against aphis and mites].an assay was made on the bioactivity of the extracts of tangerine peel from cinocitrus tangerina, orange peel from citrus sinensis and mixed tangerine peel from cinocitrus sp. against aphis semia phis heraclei, aphis craccivora, uroleucon gobonis and myzus persicae using residual film or topical method, and against mites tetranychus viennensis and t. trancatus using slide-dip or immersion method. test results show that these extracts have strong bioactivity against aphids and mites. the correcte ...19957576134
aphid transmission of beet western yellows luteovirus requires the minor capsid read-through protein p74.beet western yellows luteovirus is obligately transmitted by the aphid myzus persicae in a circulative, non-propagative fashion. virus movement across the epithelial cells of the digestive tube into the hemocoel and from the hemocoel into the accessory salivary glands is believed to occur by receptor-mediated endocytosis and exocytosis. virions contain two types of protein; the major 22 kda capsid protein and the minor read-through protein, p74, which is composed of the major capsid protein fuse ...19957882968
synthesis of a full-length infectious cdna clone of cucurbit aphid-borne yellows virus and its use in gene exchange experiments with structural proteins from other luteoviruses.a full-length cdna of cucurbit aphid-borne yellows virus (cabyv) has been constructed and expressed either as an in vitro transcript, under control of a bacteriophage t7 rna polymerase promoter, or in vivo, under control of the cauliflower mosaic virus 35s promoter in an agroinfection vector. the biological activity of the cloned cdna was demonstrated by the ability of its in vitro transcript to replicate in protoplasts and of the agroinfection vector to infect agroinoculated plants. virus in th ...19958525610
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