Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| transcriptome analysis of the small brown planthopper, laodelphax striatellus carrying rice stripe virus. | rice stripe virus (rsv), the type member of the genus tenuivirus, transmits by the feeding behavior of small brown planthopper (sbph), laodelphax striatellus. to investigate the interactions between the virus and vector insect, total rna was extracted from rsv-viruliferous sbph (rvls) and non-viruliferous sbph (nvls) adults to construct expressed sequence tag databases for comparative transcriptome analysis. over 30 million bases were sequenced by 454 pyrosequencing to construct 1,538 and 953 of ... | 0 | 25288960 |
| the brain of newly hatched chicks as a host-virus system for biological studies on the rous sarcoma virus (rsv). | 1956 | 13286703 | |
| modification of tumor-response to rous sarcoma virus (rsv) by hydrocortisone. | 1956 | 13297724 | |
| importance of the infecting dose on growth patterns of rous sarcoma virus (rsv) in chick brain. | 1960 | 13739819 | |
| quantitative studies on rous sarcoma virus. v. an analysis of the mechanism of virulence of the bryan "high titer" strain of rsv. | 1960 | 14434915 | |
| comparative studies in rous sarcoma with virus, tumor cells and chick embryo cells transformed in vitro by virus : iii. malignancy in vivo of cells transformed in vitro by virus. | chick embryo fibroblasts infected with rous sarcoma virus in vitro are rendered malignant for such cells produce typical rous sarcomas when injected into susceptible chicks since the tumors produced predominantly retain the sex chromatin patterns of the donor cells when such cells are injected into a recipient of the opposite sex. however, examination of the sex chromatin of cells at the periphery of the tumor shows presence of recipient cells though the bulk of the tumor is clearly of donor cel ... | 1962 | 19867215 |
| a macrofocus assay for rous sarcoma virus (rsv) in chicken embryo cells. | 1963 | 14070181 | |
| analysis of the defectiveness of rous sarcoma virus, ii. specification of rsv antigenicity by helper virus. | 1964 | 14104603 | |
| age effects on the host and tissue response to rous sarcoma virus (rsv). | 1964 | 14184619 | |
| a review of recent studies on rous sarcoma virus (rsv) emphasizing virus-cell-host interactions. | 1964 | 14269510 | |
| clonal response of chick fibroblasts to infection with rous sarcoma virus (rsv). | 1965 | 14279065 | |
| analysis of the defectiveness of rous sarcoma virus. 3. determining influence of a new helper virus on the host range and susceptibility to interference of rsv. | 1965 | 14297212 | |
| further studies on specific transplantation antigens in rous sarcoma of mice. | mice allografted with different sarcomas, induced by the schmidt-ruppin variant of rous sarcoma virus (rsv-sr), showed a resistance against subsequent isografting of 9 different rous sarcomas. transplantation resistance could also be induced by rous mouse tumor cells x-irradiated with 8000 r or with cell-free tumor extracts, containing no demonstrable virus. no transplantation resistance could be demonstrated after allograft pretreatment with various polyoma tumors or non-viral tumors. allograft ... | 1965 | 14316951 |
| suppression of rous sarcoma virus growth in tissue cultures by mycoplasma orale. | somerson, norman l. (national institute of allergy and infectious diseases, bethesda, md.), and m. k. cook. suppression of rous sarcoma virus growth in tissue cultures by mycoplasma orale. j. bacteriol. 90:534-540. 1965.-an agent which produced cell destruction in human diploid and chick-embryo fibroblasts was isolated from wi-26 strain of human diploid fibroblasts and shown to be a mycoplasma. the multiplication of rous sarcoma virus (rsv) and rous associated virus (rav) was inhibited in wi-26, ... | 1965 | 14329470 |
| specific transplantation immunity in relation to rous sarcoma virus tumorigenesis in mice. | tests for transplantation immunity and for the occurrence of virus-neutralizing serum antibodies were performed on mice, inoculated when newborn with the schmidt-ruppin strain of rous sarcoma virus (rsv-sr). mice developing no palpable primary sarcomas showed a clear-cut resistance against the isografting of established specifically antigenic rous tumors. transplantation tests performed on primary tumor hosts after extirpation of the tumors revealed neither any clear-cut immunity nor tolerance t ... | 1966 | 4287060 |
| the mechanism of interference between an avian leukosis virus and rous sarcoma virus. ii. early steps of infection by rsv of cells under conditions of interference. | 1966 | 4287699 | |
| analysis of defectiveness of rous sarcoma virus. iv. kinetics of rsv production. | the growth kinetics of a defective strain of rsv was studied under two different conditions. in the first, helper viruses having different growth rates were added to non-virus-producing rous cells in which infection by rsv without helper virus had been established. in the second, rsv was added to chick embryo cells which had previously been infected with helper virus and which were, therefore, in full production of viral coats. in the first instance, the initial rate of rsv production was regula ... | 1966 | 18611468 |
| studies on rous sarcoma virus in mice. ii. clonal analysis of cell populations of the sr-rsv-induced mouse ascites sarcoma (sr-c3h/he ascites). | 1967 | 4294688 | |
| comparative studies with the zilber (carr) strain of rous sarcoma virus (z-rsv). 3. suppression of viral replication and focus formation by interferon in vitro. | 1967 | 4299770 | |
| growth curve and distribution of rous sarcoma virus (bryan) in japanese quail. | on primary infection with the bryan strain of rous sarcoma virus (rsv), the growth curve of the virus in the brain of japanese quail was similar to that observed in chicks and turkey poults. infectious virus disappeared from the brain after inoculation. after an eclipse period during which no virus was detectable, infectious virus began to appear at 2 days and reached maximal titers in the brain samples at 7 days after inoculation. when japanese quail were infected intracerebrally with rsv, rela ... | 1967 | 4316245 |
| comparative studies with the zilber (carr) strain of rous sarcoma virus (z-rsv). i. response of chicken embryo fibroblasts to infection with z-rsv. | 1967 | 4382475 | |
| comparative studies with the zilber (carr) strain of rous sarcoma virus (z-rsv). ii. antigenic relationships and cyst producing capacity in newborn rats. | 1967 | 4382988 | |
| genetic variation in the ability of a planthopper vector; laodelphax striatellus (fallén) to acquire the rice stripe virus. | 1967 | 6025062 | |
| the mechanism of genetic resistance of chick embryo cells to infection by rous sarcoma virus-bryan strain (bs-rsv). | the early steps of rous sarcoma virus-bryan strain (bs-rsv) infection of genetically resistant and susceptible chick embryo cells were investigated. the methods used were the uptake of virus by cells grown in monolayer, and the ability of infected cells to form foci of transformed cells after plating on resistant and susceptible assay plates. it was found that the initial attachment of virus to either genetic type of cells did not differ appreciably. attachment on susceptible cells was immediate ... | 1967 | 18614070 |
| ultrastructure of the surfaces of cells infected with avian leukosis-sarcoma viruses. | when stained with ruthenium red (rr), chick embryo cells infected with various strains of rous sarcoma virus (rsv) and with avian leukosis viruses rav-1 and rav-3 showed an increase in the layer of acid mucopolysaccharides (amps) at their surfaces as compared with uninfected cells. this increase was most prominent in cells infected with the fujinami strain of rsv. the layer was resistant to digestion with neuraminidase or trypsin but was readily removed by exposure to hyaluronidase. the thicknes ... | 1968 | 4177656 |
| quantitative assay system for the transmission of rsv genome from virogenic mammalian cells into the chicken cell with the help of the sendai virus. | 1968 | 5653136 | |
| an electron microscopic study of the chorioallantoic membrane following infection with rous sarcoma virus. | infection of the chick chorioallantoic membrane (cam) with rous sarcoma virus (rsv) has been thought by earlier workers (12, 20) to result in the transformation of the ectoderm and then the mesoderm of that organ. in the present study, cam were infected with 10(4) pfu (pock-forming units) of rsv (bryan high titre strain) and collected for electron microscopy at 2, 4, and 6 days postinfection. observations of the fine structural changes in the cam after rsv infection support a singular role of th ... | 1968 | 4295643 |
| use of the nucleic acid hybridization method for the demonstration of dna homologous to rous virus rna in rsv-transformed mammalian cells. | 1969 | 4307533 | |
| studies on rous sarcoma virus in mice. 3. three lines of sr-rsv-induced mouse ascites sarcoma. | 1969 | 4312682 | |
| hybridization of rna from rous sarcoma virus with cellular and viral dna's. | the rna of rous sarcoma virus (rsv) hybridizes with dna's isolated from all eukaryotes tested (animal and plant) with the possible exception of yeast. bacterial dna's show no homology to rsv-rna. dna's from the oncogenic viruses, adenovirus type 12 and sv40 show significant homology with rsv-rna, but those from nononcogenic adenoviruses types 2 and 4 are not homologous.rsv-rna hybridized to all of the dna's examined has the same, or a closely similar, base composition. moreover, the melting temp ... | 1969 | 4313332 |
| presence of viral genome in chinese hamster embryonic cell transformed in vitro with bryan strain of rous sarcoma virus (b-rsv). | 1969 | 4314438 | |
| virus-specific antigens in hamster cells transformed by rous sarcoma virus. | virus-specific antigens were studied in hamster cells transformed by rous sarcoma virus (rsv). antigens were localized in the cytoplasm, as demonstrated by fluorescent antibody staining of fixed cells as well as by complement fixation (cf) following subcellular fractionation. cytoplasmic extracts were analyzed by velocity and isopycnic centrifugation. cf antigens were found in a soluble form and in association with membranes and polyribosomes. isolated plasma membranes had no cf antigen. both so ... | 1970 | 4315157 |
| investigations on virus production in rsv mammalian tumours. | 1970 | 4315847 | |
| occurrence of phentypically mixed viruses in the bryan high-titer strain of rous sarcoma virus and rsv (rav-1) stocks. | 1970 | 4312714 | |
| pathological changes in virus infections of the lower respiratory tract in children. | the pathological changes are described in 22 children with proven or suspected virus infection of the lower respiratory tract. two main patterns of disease were found: acute bronchiolitis and interstitial pneumonia. particular viruses were not specifically associated with particular histological changes. the prime importance of the respiratory syncytial virus (rsv) as a cause of disease and death in young infants is again shown. structural lesions and clinical dysfunction correlate fairly well; ... | 1970 | 4909103 |
| [infectivity for mice of the ribonucleic acid extracted from chicken sarcoma induced by the carr (zilber) strain of rous sarcoma virus (rsv-carr-zilber-rna). i. morphological study]. | 1970 | 4327545 | |
| characteristics of variants of rous sarcoma virus (rsv) isolated from mouse rsv tumors. | 1970 | 4317738 | |
| a cell-associated factor essential for formation of an infectious form of rous sarcoma virus. | formation of a specific type of infectious rous sarcoma virus called rsv(0) has been found to occur only in a certain type of chick embryo cell in the absence of avian leukosis virus. although these chick cells lack any complete form of avian leukovirus, they appear to carry a genetic factor essential for formation of infectious rsv(0). a factor-deficient cell can be converted to a producer of infectious rsv(0) by infection with rous sarcoma virus or avian leukosis virus which has been grown in ... | 1970 | 4317912 |
| mechanism of oncogenic transformation by rous sarcoma virus. i. intracellular inactivation of cell-transforming ability of rous sarcoma virus by 5-bromodeoxyuridine and light. | chick embryo fibroblasts brought into stationary phase of growth by maintenance in serum-free eagle's mem medium were infected with the bryan strain of rous sarcoma virus (b-rsv) and incubated for 18 hr in the presence of 5-bromo-deoxyuridine (budr). the cells were then allowed to resume growth and deoxyribonucleic acid (dna) synthesis by addition of an enriched f12 medium containing serum and rsv antibody to prevent spread of viral infection. after 48 hr, the cultures were exposed for various p ... | 1970 | 4318089 |
| complementarity between rous sarcoma virus (rsv) rna and the in vitro-synthesized dna of the virus-associated dna polymerase. | 1970 | 4320669 | |
| recovery of a new virus from apparently normal chick cells by infection with avian tumor viruses. | cells derived from the majority of chick embryos, although free of presently known avian tumor virus particles, appear to contain genetic materials similar to those found in this virus group. after infection of these cells with avian leukosis or rous sarcoma virus, the genetic factor was recovered by incorporation into mature infectious virus. the newly isolated virus (rav-60) did not require the assistance of another virus for its replication. the virus had most of the attributes of an rna-cont ... | 1970 | 4321346 |
| further characterization of the cellular dna hybridizing with the rna of rous sarcoma virus. | both 70s and 4s rnas of rous sarcoma virus (rsv) contain nucleotide sequences homologous to those in cellular dna. preparations of these rnas were found to include some adventitious cellular srna. nevertheless, both 70s and 4s rsv-rnas were found by competition techniques to contain sequences distinct from those in cellular ribosomal rna and 4s rna. competition experiments also reveal substantial homology between 70s and 4srsv-rna. in hybridization experiments performed in formamide at low tempe ... | 1971 | 4323787 |
| comparison of rous sarcoma virus-specific deoxyribonucleic acid polymerases in virions of rous sarcoma virus and in rous sarcoma virus-infected chicken cells. | labeled virions of rous sarcoma virus (rsv) were disrupted with detergent and analyzed on equilibrium sucrose density gradients. a core fraction at a density of approximately 1.24 g/cc contained all of the (3)h-uridine label and about 30% of the (3)h-leucine label from the virions. endogenous viral deoxyribonucleic acid (dna) polymerase activity was only found in the same location. additional ribonucleic acid (rna)- and dna-dependent dna polymerase activities were found at the top of the gradien ... | 1971 | 4326752 |
| deoxyribonucleic acid polymerase(s) of rous sarcoma virus: effects of virion-associated endonuclease on the enzymatic product. | purified preparations of rous sarcoma virus (rsv) contain ribonuclease which is either a constituent of the virion surface or an adsorbed contaminant. treatment of the virus with nonionic detergent to activate ribonucleic acid (rna)-dependent deoxyribonucleic acid (dna) polymerase renders the viral genome susceptible to hydrolysis by the external ribonuclease. the extent of this susceptibility can be substantially reduced by the use of limited amounts of detergent. at a concentration of detergen ... | 1971 | 4328411 |
| malignant and transforming activity of rous sarcoma virus (rsv). 3. detection of tumor-specific transplantation and membrane antigens in mouse cell lines transformed in vitro by rsv. | 1971 | 4328951 | |
| dna in uninfected and virus-infected cells complementary to avian tumor virus rna. | the 70s rna component of several avian tumor viruses was hybridized with dna extracted from avian tumor virus-infected and uninfected chicken and japanese quail cells. tritium-labeled 70s rnas from rous sarcoma virus (rsv), rous associated virus-1 (rav-1), rav-60, and schmidt-ruppin-rsv (sr-rsv) hybridize from 3 to 10 times more with dna from uninfected chicken cells than with dna from escherichia coli, calfthymus, or baby hamster kidney cells. after infection of chicken cells with rsv(rav-1), s ... | 1971 | 4332808 |
| [infectivity for mice of ribonucleic acid extracted from chicken sarcoma induced with the carr (zilber) strain of rous sarcoma virus -rsv-(zilber) rna-carr-. ii. histoenzymological study]. | 1971 | 4335015 | |
| an avian leukosis virus related to rsv(o): properties and evidence for helper activity. | 1971 | 5101017 | |
| cell transformation induced by rous sarcoma virus: analysis of density dependence. | trypsinized chick embryo cells will divide at least once when seeded onto stationary, preexisting cultures of chick cells, but not when seeded onto stationary mouse cells. it is suggested that the mouse cells reconstitute a surface component for the trypsinized chick cells. these findings explain why crowded mouse cell layers, but not chick cell layers, suppress the transformation of chick cells infected with rous sarcoma virus (rsv), since the infected cells must proceed through one cell cycle ... | 1971 | 18619369 |
| hybridization of rous sarcoma virus deoxyribonucleic acid polymerase product and ribonucleic acids from chicken and rat cells infected with rous sarcoma virus. | rous sarcoma virus (rsv)-specific ribonucleic acid (rna) in virus-producing chicken cells and non-virus-producing rat cells infected with rsv was studied by hybridization with the endogenous deoxyribonucleic acid (dna) product of the rsv virion dna polymerase system. by hybridizing the total dna product with excess virion rna, the product dna was separated into hybridized ("minus") and nonhybridized ("plus") dna. the "minus" dna was complementary to at least 20% of the rna from rsv which remaine ... | 1972 | 4337163 |
| [electron microscope study of the fixation of lectins labelled with raifort peroxidase on human embryonic cells transformed in vitro by the rous sarcoma virus (rsv), bryan strain]. | 1972 | 4339174 | |
| role of subunits of 60 to 70s avian tumor virus ribonucleic acid in its template activity for the viral deoxyribonucleic acid polymerase. | heating the 60 to 70s ribonucleic acid (rna) of rous sarcoma virus (rsv) destroys both its subunit structure and its high template activity for rsv deoxyribonucleic acid (dna) polymerase. in comparative analyses, it was found that the template activity of the rna has a thermal transition of 70 c, whereas the 60 to 70s structure dissociates into 30 to 40s and several distinct small subunits with a t(m) of 55 c. analysis by velocity sedimentation and isopycnic centrifugation of the primary dna pro ... | 1972 | 4339194 |
| cell cycle-dependent activation of rous sarcoma virus-infected stationary chicken cells: avian leukosis virus group-specific antigens and ribonucleic acid. | stationary chicken embryo fibroblasts exposed to rous sarcoma virus (rsv) remained stably infected for at least 5 days, but they did not release infectious virus or become transformed until after cell division. these infected stationary cells did not contain avian leukosis virus group-specific antigens or ribonucleic acid (rna) hybridizable to deoxyribonucleic acid (dna) made by the rsv endogenous rna-directed dna polymerase activity. | 1972 | 4339198 |
| temperature-dependent transformation of cells infected with a mutant of bryan rous sarcoma virus. | chick embryo cells infected with a mutant (ta) of the bryan high-titer strain of rous sarcoma virus (rsv-bh) are morphologically transformed at 36 c but appear similar to uninfected cells at 41 c. when cells infected with rsv-bh-ta are switched from 41 to 36 c, morphological changes characteristic of transformation are observable within 10 min. the transformation is reversible; cells shifted from 36 to 41 c have been observed to lose their transformed morphology within 1 hr. the transformation a ... | 1972 | 4342244 |
| dna copies of viral rna in rat cells transformed by rous sarcoma virus (rsv). | 1972 | 4344277 | |
| [agglutination by concanavalin a of chick embryo fibroblasts transformed by the rous sarcoma virus (sr-rsv) and a thermosensitive mutant of this virus]. | 1972 | 4334590 | |
| reverse transcriptase-containing particles induced in rous sarcoma virus-transformed rat cells by arginine deprivation. | incubation of rat cells transformed by rous sarcoma virus (rsv) in an arginine-deficient medium resulted in accumulation of particles in the culture medium. such particles did not appear when the transformed rat cells were incubated in a complete medium nor in the medium of primary rat cells which were incubated either in arginine-deficient or complete media. the particles which were released from the arginine-deprived transformed rat cells resemble c-type particles in their properties. these pa ... | 1972 | 4116137 |
| an enzymatic function associated with transformation of fibroblasts by oncogenic viruses. ii. mammalian fibroblast cultures transformed by dna and rna tumor viruses. | chick, hamster, mouse, and rat embryo fibroblast cultures, transformed by either dna or rna viruses, show fibrinolytic activity under suitable conditions of growth and in appropriate media; normal counterpart cultures do not. the fibrinolysin is produced by the interaction of two protein factors: one of these, a cell factor, is released by transformed cells and accumulates in the medium when cultures are incubated in the absence of scrum. the second factor, the serum factor, is a specific protei ... | 1973 | 4347288 |
| an enzymatic function associated with transformation of fibroblasts by oncogenic viruses. i. chick embryo fibroblast cultures transformed by avian rna tumor viruses. | chick embryo fibroblast cultures develop fibrinolytic activity after transformation by rous sarcoma virus (rsv). this fibrinolytic activity is not present in normal cultures, and it does not appear after infection with either nontransforming strains of avian leukosis viruses or cytocidal rna and dna viruses. in cultures infected with a temperature sensitive mutant of rsv the onset of fibrinolysis appears after exposure to permissive temperatures and precedes by a short interval the appearance of ... | 1973 | 4347290 |
| chromatographic and electrophoretic analysis of viral proteins from hamster and chicken cells transformed by rous sarcoma virus. | several methods have been explored for the detection and characterization of viral proteins from soluble extracts of cells transformed by rous sarcoma virus (rsv). viral antigens have been analyzed after gel filtration in several solvents. in addition, immune complexes formed with virus-specific sera have been isolated by agarose gel filtration and by high- or low-speed centrifugation through sucrose solutions. radioactive proteins from these immune complexes have been analyzed by gel filtration ... | 1973 | 4347428 |
| role of mitochondria in the production of rna-containing tumor viruses. | the role of mitochondria in the reproduction of rna-containing tumor viruses was examined by using ethidium bromide (eb) to induce degenerative effects in mitochondria. the effects of eb in murine and avian cells were monitored by electron microscopy. chronically infected mouse (jls-v5) cells, in which extensive mitochondrial changes were induced, continued to produce murine leukemia virus. also, complete reproductive cycles of rous sarcoma virus (rsv) occurred in newly infected chicken embryo c ... | 1973 | 4347431 |
| relationship of reticuloendotheliosis virus to the avian tumor viruses: nucleic acid and polypeptide composition. | the rna content and polypeptide composition of reticuloendotheliosis virus (rev) was compared to that of c-type rna tumor viruses. two rna species with approximate sedimentation values of 64s and 4s were observed after sucrose gradient centrifugation of rna extracted from purified rev. the high-molecular-weight rna species of rev sedimented slightly faster than that of the bryan strain of rous sarcoma virus (rsv). although these characteristics were consistent with those of other c-type rna tumo ... | 1973 | 4350718 |
| sugar transport in normal and rous sarcoma virus-transformed chick-embryo fibroblasts. | 3-o-methylglucose (3-omeg) is a nonmetabolizable glucose analog and is, therefore, suitable for transport studies. 3-omeg and glucose compete for entry into normal and rous sarcoma virus (rsv)-transformed chick-embryo fibroblasts. therefore, 3-omeg can be used to study the transport of glucose in these cells. chickembryo fibroblasts infected and transformed by rsv take up 3-omeg at a faster rate than uninfected cells when both cell types are growing at the same rate. the rate of efflux of 3-omeg ... | 1973 | 4351798 |
| transformation of chick-embryo fibroblasts by wild-type and temperature-sensitive rous sarcoma virus alters adenylate cyclase activity. | the activity of the enzyme adenylate cyclase, a component of the plasma membrane, has been determined in chick-embryo fibroblasts and in cells transformed by either bryan high-titer strain of rous sarcoma virus (rsv-bh) or a temperature-sensitive mutant of this virus (rsv-bh-ta). adenylate cyclase activity is reduced in cells transformed by the wild-type virus and also by the temperature-sensitive mutant when the cells are grown at the permissive temperature (37 degrees ). transformation results ... | 1973 | 4352222 |
| safety and antigenicity of temperature sensitive (ts) mutant respiratory syncytial virus (rsv) in infants and children. | 1973 | 4353352 | |
| dna polymerase of murine sarcoma-leukemia virus: lack of detectable rnase h and low activity with viral rna and natural dna templates. | kirsten murine sarcoma-leukemia virus (ki-msv[mlv]) was found to contain less rnase h per unit of viral dna polymerase than avian rous sarcoma virus (rsv). upon purification by chromatography on sephadex g-200 and subsequent glycerol gradient sedimentation the avian dna polymerase was obtained in association with a constant amount of rnase h. by contrast, equally purified dna polymerase of ki-msv(mlv) and moloney [mo-msv(mlv)] lacked detectable rnase h if assayed with two homopolymer and phage f ... | 1973 | 4357518 |
| genetic control of susceptibility to an a subgroup sarcoma virus in commercial chickens. | sykes line a white leghorn and line b rhode island red embryos had differences in the average pock counts on the chorio-allantoic membrane (cam) after infection with the bryan strain of rous sarcoma virus (bs-rsv). by crossing the two lines 2610 embryos comprising 38 sire families were obtained. after challenge of their cams with bs-rsv analysis of the number of resulting pocks was made to determine whether or not the cam response, a numerical trait, was controlled by simple mendelian inheritanc ... | 1973 | 18777380 |
| nucleotide sequence relationships of avian rna tumor viruses: measurement of the deletion in a transformation-defective mutant of rous sarcoma virus. | stocks of cloned helper-independent rous sarcoma virus (rsv) spontaneously segregate transformation-defective (td) mutants that appear to have an rna genome composed of smaller subunits than those of the patent virus. differential hybridization and competitive hybridization techniques involving reactions between viral rna and proviral sequences in host cell dna (under conditions of initial dna excess) were used to measure the extent of the deletion in a td mutant of prague strain (pr) of rsv (pr ... | 1974 | 4362512 |
| virus productive transformation of marsupial cells by schmidt-ruppin strain of rsv. | 1974 | 4363168 | |
| requirement for cell division for initiation of transcription of rous sarcoma virus rna. | stationary chicken embryo fibroblasts exposed to rous sarcoma virus (rsv) did not synthesize virus rna until after division of the infected cells. initiation of rna transcription after cell division resulted in transcription of the entire viral genome and was followed shortly thereafter by appearance of progeny virus. addition of colchicine to rsv-infected stationary chicken embryo fibroblasts resulted in inhibition of cell division and a 24-h delay in the appearance of virus rna. rsv-infected c ... | 1974 | 4369144 |
| sarcoma and transformation-defective viruses produced with infectious dna(s) from rous sarcoma virus (rsv)-transformed chicken cells. | 1974 | 4371384 | |
| distribution of fibroblast surface antigen: association with fibrillar structures of normal cells and loss upon viral transformation. | the localization of a cell type-specific, soluble fibroblast surface antigen (sfa) was studied by immunofluorescence and by scanning electron microscopy of the same cells. the antigen had an uneven distribution forming streaks on chick embryo fibroblasts. it was localized to membrane processes and ridges, with a diameter of 50-200 nm. the processes extended from the periphery of the cells to the substratum or to other cells. trypsin treatment completely removed detectable amounts of sfa. the ant ... | 1974 | 4372293 |
| results of investigations concerning the oncogenicity for mice and hamsters of nuclei acid preparations extracted from chicken sarcoma induced by the carr (zilber) and bryan strains of rous sarcoma virus (rsv). | 1974 | 4377313 | |
| transcription of dna from the 70s rna of rous sarcoma virus. i. identification of a specific 4s rna which serves as primer. | the enzymatic transcription of dna from the 70s rna of rous sarcoma virus (rsv) is initiated on the 3' terminus of a molecule of 4s rna which is hydrogen bonded to the viral genome. we labeled this primer with radioactive deoxynucleotides, and demonstrated that its release from 70s rna by thermal denaturation was accompanied by a reduction in the template activity of the viral rna. two-dimensional electrophoresis in polyacrylamide gels separated the 4s rnas associated with the 70s rna of rsv int ... | 1974 | 4132919 |
| separation and concentration of respiratory syncytial virus (rsv) antigens. | 1974 | 4827015 | |
| clinically useful method for the isolation of respiratory syncytial virus. | a simple method for the isolation of respiratory syncytial virus (rsv) is reported; it is relatively rapid and results in a high frequency of recovery of virus. a nasal secretion specimen with high titers of virus is inoculated at the bedside onto susceptible cell lines to avoid loss of viral infectivity due to liability of the virus. during an outbreak of rsv, viral specimens were obtained by this method from all young children admitted to the hospital with lower respiratory tract disease. rsv ... | 1975 | 162921 |
| immunization of rous sarcoma virus-inoculated marmosets with bcg and transformed allogeneic cells. | the effects of specific immunotherapy with allogeneic cells transformed by schmidt-ruppin rous sarcoma virus (sr-rsv), of treatment with bcg, and of surgery on the growth of sr-rsv-induced sarcomas in white-lipped marmosets were studied. tumor incidence, tumor progression, and survival did not differ between control and treated animals. animals immunized with bcg developed lymphocyte reactivity to tuberculin, which remained until the animals died. bcg was isolated from the spleen of one tumor-be ... | 1975 | 163312 |
| molecular weight of rna subunits of rous sarcoma virus determined by electron microscopy. | secondary cultures of chicken embryo fibroblasts were infected with the schmidt ruppin strain of rous sarcoma virus (rsv). five days after infection, the medium was replaced at 2-h intervals with phosphate-free eagle medium containing 50 muci of [32p]orthophosphate per ml. virus was collected by centrifugation, and the rna was extracted and denatured with dimethyl sulfoxide, and the 33s subunit rna was isolated by sucrose gradient centrifugation. the molecular weight of the rsv subunit rna was d ... | 1975 | 163340 |
| an outbreak of severe pneumonia due to respiratory syncytial virus in isolated arctic populations. | a rapidly developing outbreak of pneumonia in young infants was documented in two isolated artic populations in may 1972. these were studied virologically, serologically and clinically. in addition to the two stricken communities, one apparently unaffected with serious clinical illness and a fourth, in which are located the major hospital and airport in the eastern arctic, were also studied. one hundred and twenty-four patients were studied serologically and 81 respiratory and other specimens we ... | 1975 | 163574 |
| chicken leukosis virus genome sequences in dna from normal chick cells and virus-induced bursal lymphomas. | genome sequences of two recent field isolates of avian leukosis viruses in the dna of normal and neoplastic chicken cells were studied by dna-rna hybridization under conditions of dna excess. comparisons were made between 60-70s rna from these viruses and that of a chicken endogenous type c virus (rav-0), and of a series of "laboratory" leukosis and sarcoma viruses, by competitive hybridization analysis. a minimum of 18% of the genome sequences of both alv isolates detected in dna from lymphomas ... | 1975 | 164291 |
| potential of attenuated respiratory syncytial virus vaccine for infants and children. | respiratory syncytial virus (rsv) disease is a major cause of death and hospitalization in infancy and a frequent cause of morbidity throughout childhood. serum antibody does not protect as is evident from the study of natural disease and use of killed vaccines. local antibody responses occur in natural illness. possibly serum antibody in the absence of local antibody plays a part in illness. we have studied local and serum antibody response to potential attenuated vaccines: a 26 degrees c adapt ... | 1975 | 165119 |
| infectious dna recovered from avian tumor-virus-producing cells. | a single treatment of chick embryo fibroblasts with dna recovered from chick embryo fibroblasts productively infected and transformed with four different strains of rsv, or productively infected with two different strains of rav, resulted in virus production and cell transformation (in the case of rsv) two or three passages after treatment (8-25 days). the virus recovered from cultures was phenotypically identical to that produced by the donor cells. no virus production nor cell transformation ... | 1975 | 165150 |
| rna of replication-defective strains of rous sarcoma virus. | the rna of a replication-defective (rd) mutant, isolated from stocks of nondefective (nd) schmidt-ruppin rous sarcoma virus of subgroup a (sr-a) and termed sr-n8, was compared to the rnas of sr-a, of a transformation-defective derivative of sr-a (td sr-a) and of rd bryan rous sarcoma virus, rsv (minus). the molecular mass of the 30-40s species of sr-n8 rna was estimated to be 21% (congruent to 7.5 to 8 times 10-5 daltons) smaller than that of sr-a by (i) electrophoresis in polyacrylamide gels an ... | 1975 | 165514 |
| effect of regression of rous sarcoma tumors upon egg production in an inbred line of white leghorns. | reserach was conducted to determine whether development and subsequent regression of a rous sarcoma virus (rsv) induced wing-web tumor influenced egg production. fifty-seven six-week old pullet chicks of inbred line 6 of the united states department of agriculture, regional poultry research laboratory, east lansing, michigan, were inoculated subcutaneously in the left wing-web with 0.1 ml. of a 10-minus 3 dilution of a pseudotype of bryan high titer rsv designated bh-rsv (rav-1). thirty chicks ... | 1975 | 166364 |
| evidence for a host cell surface antigen on the envelope of avian tumour viruses. | avian sarcoma viruses of the a, b, c, d and e subgroups are inactivated about 100-fold by the serum of rabbits immunized against chick embryo (ce) cells, in the presence, but not in the absence, of complement. the inactivation is not due to the action of the antiserum and complement on the ce cell cultures used for virus assay, nor to anti-forssman antibodies, but it is presumably due to antibodies to some antigen(s) common to the surface of ce cells and to the virus envelope. this host cell sur ... | 1975 | 167111 |
| further evidence for the existence of a viral envelope protein defect in the bryan high-titer strain of rous sarcoma virus. | electron microscopy observations of purified bryan high-titer rous sarcoma virus (bh rsv) using the freeze-drying technique showed that progeny made in the absence of a helper virus lacked visible surface projections or spikes. phenotypic mixing experiments employing bh rsv and a thermolabile mutant of vesicular stomatitis virus, tl 17, yielded no evidence of pseudotype formation. since tl 17 is known to be defective for an envelope glycoprotein, the lack of successful phenotypic mixing with bh ... | 1975 | 168409 |
| phenotypic mixing test to detect and assay avian leukosis viruses. | a phenotypic mixing (pm) test for detecting and assaying avian leukosis viruses (alv) of the a, b, c, and d subgroups is described. an alv and rous sarcoma virus rsv-0) are phenotypically mixed by co-cultivating on c/o (cells susceptible to all subgroups of alv) cells for a certain period. then the rsv with the new virus property is assayed on c/e cells (cells resistant to infection with subgroup e leukosis/sarcoma viruses). the test is relatively simple and rapid, and its results are unequivoca ... | 1975 | 168850 |
| infectious viral dna in rous sarcoma virus-transformed nonproducer and producer animal cells. | nonproducer and producer rsv-transformed cells and producer nontransforming virus-infected cells harbor viral dna specifying the respective avian tumor virus. in nonproducer rous sarcoma cells, the residing viral dna is linear, double-stranded and covalently linked to the chromosomal dna. both double-stranded and single-stranded forms of rsv dna transfect chicken cells. the progeny virus is indistinguishable from the dna parent with respect to the morphological, biological and antigenic properti ... | 1975 | 169003 |
| infectious dna from cells infected with rous sarcoma virus, reticuloendotheliosis virus or rous-associated virus-o. | we have described an efficient and quantitative assay for infectious dna of the avian ribodeoxyviruses and have applied this method to study the possible existence of infectious viral dnas in uninfected cells. infectious dna from cells infected with rsv or rev consisted of a single unit of dna with a specific infectivity of 10(-5)-10(-6). the minimum molecular weight of rsv dna required for infection of chicken cells was about 6 million, while the minimum molecular weight of infectious rev dna w ... | 1975 | 169004 |
| 35s a and b rna subunits of avian rna tumor virus strains cloned and passaged in chick and duck cells. | the rna of transforming and nontransforming avian rna tumor viruses was heat denatured and examined for its 35s subunit composition by polyacrylamide gel electrophoresis (gel slicing and/or autoradiography). the rna of virus strains capable of transforming chick embryo fibroblasts in vitro always contained a subunits: the rna of pr-rsv of subgroups a, b or c was composed only of a subunits, even after prolonged passage in chick cells, whereas the rna of sr-rsv-a, sr-rsv-b, sr-rsv-d(h) and b77 al ... | 1975 | 169040 |
| genetic recombination among temperature-sensitive mutnats of rous sarcoma virus. | genetic recombination of rsv has been studied, using ts mutations in both initiation and maintenance of transformation as markers. the progeny of a single cycle of mixed infection appears to contain no recombinants, but yields heterozygous particles or viral clumps. on subsequent cycles of infection some of these heterozygotes/clumps persist, but they also segregate recombinant viruses. some of the markers in these recombinants show evidence of linkage and thus probably recombine by intramolecul ... | 1975 | 169044 |
| plaque assay of avian sarcoma viruses using casein. | the caseinolytic activity of several strains of rous sarcoma virus (rsv), conditional and nonconditional mutants of rsv, and nontransforming avian leukosis viruses was investigated. only those viruses capable of transforming chick fibroblasts in vitro induced lysis of casein incorporated into an agar overlay. lysis produced distinct clear areas in the turbid casein-agar gel which allowed a quantitative "plaque" assay of cell transformation. casein plaque formation could not be separated from mor ... | 1975 | 169393 |
| rous sarcoma regression in chickens resistant or susceptible to marek's disease. | in 4 experiments, strains of chickens relatively susceptible (s-strain, p-line) or resistant (n-line, pdrc) to marek's disease (md) were challenged with rous sarcoma virus (rsv) at 6-8 weeks old. rsv tumors occurred in 94-100% of the birds in each strain, but the number with tumors that regressed during the 40-46-day experiment varied from 7% (s-strain) to 91% (pdrc). the n-line and p-line birds, derived from a random-bred flock with selection only for susceptibility or resistance to md, regress ... | 1975 | 169801 |
| tumour-associated transplantation antigen in sera of rats with large rsv-induced sarcomas. | a factor inhibiting tumour growth in syngeneic hosts was found in the sera of inbred lewis rats carrying rous sarcoma virus-induced tumour (rsl). the findings presented here suggest that the serum factor is a tumour-associated transplantation antigen (tata) shed from the neoplasm into the circulation. all the tumour bearers' sera tested with rsl cells were negative in indirect membrane immunofluorescence;however, on passive transfer into syngeneic rats, they protected the animals against the gro ... | 1975 | 170216 |
| appearance of c-type virus-like particles after co-cultivation of a human tumor-cell line with rat (xc) cells. | a serially progagated cell line (l104) was established by co-cultivation of alung adenocarcinoma (l-1) from a patient with concurrent chronic lymphocytic leukemia and xc, a non-producer rat line, known to carry the rous sarcoma virus (rsv) genome. karyotype of the l104 cultures revealed predominantly rat-like patterns; however, about 5% of the cells reacted with hla antibodies and demonstrated human isozyme patterns. electron microscopy of l104 cells revealed the presence of c-type particles bud ... | 1975 | 170217 |
| presence of chicken cell surface antigen on rous virus activated in heterokaryons of transformed non-permissive hamster cells and chicken cells. | incubation with antiserum to chick embryo (ce) cells, in the presence of complement, inactivates rous sarcoma virus (rsv) produced by heterokaryons formed by non-permissive rsv-transformed hamster cells and ce cells as well as rsv produced by heterokaryons is observed following incubation with antiserum to the transformed hamster cells, plus complement. hence, the envelope of rsv activated in heterokaryons, as that of rsv produced by ce cells, must contain a surface antigen of the ce cell, and t ... | 1975 | 170372 |
| mapping rnase t1-resistant oligonucleotides of avian tumor virus rnas: sarcoma-specific oligonucleotides are near the poly(a) end and oligonucleotides common to sarcoma and transformation-defective viruses are at the poly(a) end. | the large rnase t1-resistant oligonucleotides of the nondefective (nd) rous sarcoma virus (rsv): prague rsv of subgroup b (pr-b), pr-c and b77 of subgroup c; of their transformation-defective (td0 deletion mutants: td pr-b, td pr-c, and td b77; and of replication-defective (rd) rsv(-) were completely or partially mapped on the 30 to 40s viral rnas. the location of a given oligonucleotide relative to the poly(a) terminus of the viral rnas was directly deduced from the smallest size of the poly(a) ... | 1975 | 170411 |
| infectious dna coding for a temperature-sensitive dna polymerase of the coordinate la335 mutant of rous sarcoma virus (rsv). | 1975 | 170738 | |
| polypeptide composition of cell membranes from chick embryo fibroblasts transformed by rous sarcoma virus. | chick embryo fibroblasts were transformed by the bryan high-titer strain of rous sarcoma virus (rsv-bh), or a mutant (rsv-bh-ta) inducing temperature-dependent transformation. surface membranes from normal and transformed cells were isolated as membrane vesicles by differential centrifugation, and as cell ghosts after zncl2 treatment and separation in an aqueous two-phase system. these preparations were analyzed by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate or p ... | 1975 | 170966 |
| interactions between endogenous baboon type-c virus and oncogenic viruses. i. syncytium induction and development of infectivity assay. | cells releasing the endogenous baboon virus (bv) can interact with human kc cells containing the rous sarcoma virus (rsv) genome, resulting in cell fusion and syncytium formation. this interaction has been utilized in the development of a sensitive infectivity assay for bv. the titration pattern is of a one-hit type, demonstrating a linear relationship between virus concentration and number of syncytial plaques obtained in the kc co-cultivation assay. endpoint titration comparisons indicate that ... | 1975 | 171228 |