Publications

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size and shape of alfalfa mosaic virus. 195813517288
a case of interference between alfalfa mosaic virus and cucumber mosaic virus. 196013729306
macromolecular particles associated with alfalfa mosaic virus. 196013796366
concentration and specific infectivity changes of alfalfa mosaic virus during systemic infection. 196114460323
association of infectivity with alfalfa mosaic virus bottom component only. 196113686525
chemical composition of partially purified alfalfa mosaic virus. 196113702123
preparation of protein subunits from alfalfa mosaic virus under mild conditions. 196214454982
x-ray studies on top component a and bottom component of alfalfa mosaic virus. 196213996037
biophysical and biochemical properties of top component a and bottom component of alfalfa mosaic virus. 196214031785
the base ratio of alfalfa mosaic virus nucleic acid. 196414166123
the base composition ribonucleic acids from alfalfa mosaic virus components. 196414187921
[inhibition of the multiplication of the myeloblastosis virus (amv) in vitro by guanidine]. 19654285130
properties of ribonucleic acid from alfalfa mosaic virus and related components. 19655850883
avian myeloblastosis virus (amv) biosynthesis in chick embryo cells: studies of the particles released by trypsin from the infected cells. 19664287875
effect of avian myeloblastosis virus in the japanese quail.moscovici, carlo (university of colorado medical center, denver), and e. h. macintyre. effect of avian myeloblastosis virus in the japanese quail. j. bacteriol. 92:1141-1149. 1966.-avian myeloblastosis virus (amv) induced a spectrum of neoplasms in japanese quail (coturnix coturnix japonica) which was similar to that observed in the chicken, with one exception: the total absence of acute myeloblastic leukemia in quail. studies in vivo as well as in vitro suggested that the cause for this differe ...19664288797
in vivo replacement of the helper virus in bryan's strain of rous sarcoma virus by amv and rpl12 viruses. 19664288867
[homology between the genome of avian myeloblastosis virus (amv) and the cellular genome]. 19664291018
enhancement of infectivity by combination of two ribonucleic acid components from alfalfa mosaic virus.rna was extracted from alfalfa mosaic virus 425. infectivity was determined in density gradient fractions and in all possible combinations of two fractions. certain combinations were remarkably infectious. this led to the purification through two cycles of sucrose gradient centrifugation of a 27 s and a 14 s rna, from bottom component and top component a, respectively. the purified bottom component rna had a low infectivity; when purified top component a rna was added, infectivity increased cons ...196718614088
the biological function and mutual dependence of bottom component and top component a of alfalfa mosaic virus. 19684967833
alfalfa mosaic virus: comparative investigation of top component a and bottom component by means of fingerprinting and immunological techniques. 19684972584
the precipitation of alfalfa mosaic virus by magnesium. 19685640827
isolation of replicative rna from alfalfa mosaic virus-infected plants by chromatography on hydroxyapatite columns. 19685653658
purification and fractionation of alfalfa mosaic virus with polyethylene glycol. 19685711434
degradation of alfalfa mosaic virus by pancreatic ribonuclease. 19695762209
[electron microscopical study of the replicative rna of alfalfa mosaic virus]. 19695772491
electron microscopy on in vivo aggregation forms of a strain of alfalfa mosaic virus. 19695776316
studies on alfalfa mosaic virus. i. the protein and nucleic acid. 19695777560
studies on alfalfa mosaic virus. ii. the structure of the virus components. 19695777561
infection of phaseolus vulgaris with alfalfa mosaic virus: inhibitory effect of pretreatment with turnip yellow mosaic virus. 19695777564
the reversible dissociation of alfalfa mosaic virus. 19695777565
separation of top and bottom components of alfalfa mosaic virus by combined differential and density gradient centrifugation. 19695782930
the production of new types of symptoms by mixtures of different components of two strains of alfalfa mosaic virus. 19695784045
amino acid analyses of two strains of alfalfa mosaic virus. 19695811915
alfalfa mosaic virus. 19695001896
distribution and appearance of alfalfa mosaic virus in infected plant cells. 19695358830
[heterotransmission of avian myeloblastoma virus (amv) to the hamster]. 19694306857
incorporation of precursors into ribonucleic acid, protein, glycoprotein, and lipoprotein of avian myeloblastosis virions.freshly explanted leukemic myeloblasts produce avian myeloblastosis virus (amv) at a constant rate without any obvious cytopathic effect; therefore, subviral components are continually synthesized at a steady rate. the incorporation of various radioactive precursors into virions was monitored by determination of radioactivity in purified virus after density equilibrium sedimentation in preformed sucrose gradients. the kinetics of incorporation of (3)h-uridine have shown that there is an average ...19694311791
an improved adenosine triphosphatase assay for physical particles of avian myeloblastosis virus (amv). 19694311838
[demonstration of virus particles of amv type in a transplantable hamster tumor: hp]. 19694318901
mechanism of carcinogenesis by rna tumor viruses. 3. formation of rna, dna complex and duplex dna molecules by the dna polymerase (s) of avian myeloblastosis virus.dna polymerase activity can be unmasked in avian myeloblastosis virus (amv) by treatment with the nonionic detergent nonidet p-40. two products are formed: (1) rna.dna hybrid molecules and (2) duplex dna molecules. the kinetics of dttp incorporation into dna are biphasic: an initial rapid reaction for 4 min at 37 degrees c with a minimal polymerization rate of 10-20 nucleotides per see, and a second reaction at about half the initial rate. viral rna.dna complexes are detected as early as 30 sec ...19704322024
dna complementary to viral rna in leukemic cells induced by avian myeloblastosis virus.nucleic acid hybridization studies were made between 71s-amv-rna and dna from leukemic myeloblasts and from normal chicken cells. there was homology between the viral rna and chicken cell dna and to a greater extent between viral rna and leukemic cell dna. leukemic cell dna hybridized approximately twice as much viral rna as did normal chicken dna. thermal melting studies showed that the viral rna bound to normal and leukemic cell dna consists of long polynucleotides (t(m) = 87 degrees and 92 de ...19704317913
studies on alfalfa mosaic virus. 3. reversible dissociation and reconstitution studies. 19705411192
aggregation of alfalfa mosaic virus under influence of externally applied agents. 19705424818
electron microscopy of the replicative rna from tobacco mosaic virus ana alfalfa mosaic virus: morphology and association of the double-stranded rna. 19705474462
electron microscopy on the behavior of two strains of alfalfa mosaic virus in mixed infections. 19705483266
[the morphology of alfalfa mosaic virus particles]. 19705517366
three nucleoprotein components of alfalfa mosaic virus necessary for infectivity. 19704985557
the in vivo behaviour of twenty-four strains of alfalfa mosaic virus. 19704098410
studies on alfalfa mosaic virus. iv. an unusual strain. 19704099064
a functional equivalence of top component a rna and coat protein in the initiation of infection by alfalfa mosaic virus. 19715124242
examination of alfalfa mosaic virus protein on polyacrylamide gels. 19715125569
self-assembly of protein subunits from alfalfa mosaic virus. 19715543287
molecular weight of the coat protein of alfalfa mosaic virus. 19715543973
alfalfa mosaic virus in lucerne plants and its transmission by aphids in the course of the vegetation period. 19714399140
efficiency of alfalfa mosaic virus transmission by aphids in relation to the level of virus in plants. 19714399142
alfalfa mosaic virus hybrids constructed by exchanging nucleoprotein components. 19724627343
distribution of deoxyribonucleic acid complementary to the ribonucleic acid of avian myeloblastosis virus in tissues of normal and tumor-bearing chickens.(3)h-labeled 70s ribonucleic acid (rna) from purified avian myeloblastosis virus (amv) was used as a probe in deoxyribonucleic acid (dna)-rna hybridization experiments to detect the presence of dna complementary to the amv genome in various tissues from noninfected normal chickens and from chickens infected with amv. there was a remarkable constancy in the average cellular concentration of virus-specific dna found in every tissue from the same uninfected chicken, and even in different chickens f ...19724344249
electron microscopy of replicative form and single-stranded rna of alfalfa mosaic virus. 19725049079
structural studies on the coat protein of alfalfa-mosaic virus. 19725050258
the replicative rna and the viral rna synthesis rate in tobacco infected with alfalfa mosaic virus. 19725053100
[symptomatologic, morphologic and ultrastructural characteristics of a strain of alfalfa mosaic virus found in spain]. 19725083209
surface-active agents for isolation of the core component of avian myeloblastosis virus.sixty-one surface-active agents were evaluated in a procedure designed to assess their ability to remove the envelope from the core component of avian myeloblastosis virus (amv). the procedure consisted of centrifugation of intact amv through a series of sucrose gradients each containing an upper layer of agent at one of eight concentrations between 0.01 and 10%. the effectiveness of an agent in producing amv cores was indicated by (i) the appearance of light-scattering bands in the region of co ...19724112071
the uncoating of alfalfa mosaic virus by its own rna. 19724113242
evidence for a ph-induced structural change of alfalfa mosaic virus. 19724117934
serological analysis of the deoxyribonucleic acid polymerase of avian oncornaviruses. i. preparation and characterization of monospecific antiserum with purified deoxyribonucleic acid polymerase.monospecific antiserum was prepared against purified deoxyribonucleic acid (dna) polymerase from avian myeloblastosis virus (amv). immunodiffusion assay with purified dna polymerase revealed that the anti-dna polymerase serum formed one precipitation band, whereas no reaction with any of the seven major structural proteins of amv was observed. the antiserum also demonstrated enzyme-neutralizing antibody activity that was associated with the immunoglobulin g fraction. there was no difference in t ...19724117965
serological analysis of the deoxyribonucleic acid polymerase of avian oncornaviruses. ii. comparison of avian deoxyribonucleic acid polymerases.monospecific antiserum prepared against the isolated deoxyribonucleic acid (dna) polymerase of avian myeloblastosis virus (amv) neutralized the endogenous ribonucleic acid-instructed dna polymerase activity of detergent-disrupted virus. the viral polymerase was serologically unrelated to the seven major structural polypeptides of amv. furthermore, the viral enzyme was distinguished from normal cellular dna polymerases by serological criteria; thus, antiserum against the viral enzyme neutralized ...19724117966
outer membrane of avian myeloblastosis virus.guinea pigs immunized intracerebrally with avian myeloblastosis virus (amv) produced antiserum which reacted with intact virus particles in complement fixation. the antigen in question appeared to be located on the surface of the virion and could be distinguished from the type-specific virus envelope and the group-specific internal antigens of chicken leukosis-sarcoma viruses (chilsv). the material could be isolated by sequential treatments of amv with bromelin, tween 20, and freeze-thawing, and ...19734128385
quantitative determination and location of newly synthesized virus-specific ribonucleic acid in chicken cells infected with rous sarcoma virus.a sensitive and quantitative nucleic acid hybridization assay for the detection of radioactively labeled avian tumor virus-specific rna in infected chicken cells has been developed. in our experiments we made use of the fact that dna synthesized by virions of avian myeloblastosis virus in the presence of actinomycin d (amv dna) is complementary to at least 35% of the sequences of 70s rna from the schmidt-ruppin strain (srv) of rous sarcoma virus. annealing of radioactive rna (either srv rna or r ...19734350719
[resistance of german chicken lines against avian myeloblastosis virus (amv)]. 19734354748
subunits of oncornavirus high-molecular-weight rna. i. stepwise conversion of 60 s amv (avian myeloblastosis virus) rna to subunits. 19734354929
chromatographic analyses of isoaccepting trnas from avian myeloblastosis virus.avian myeloblastosis virus (amv) 4s rna was tested for amino acid acceptor activity for 18 of the 20 amino acids. a nonrandom distribution of viral trnas was found compared with trna from normal liver or from amv-infected leukemic myeloblasts, confirming previous reports. methionine and proline trnas were considerably enriched, whereas glutamic acid, glutamine, serine, tyrosine, and valine trnas were markedly depleted in amv relative to homologous cellular trnas. the seven amv trnas with the gre ...19734355849
subunits of oncornavirus high-molecular-weight rna. ii. detection of double-stranded-regions in 60s amv (avian myeloblastosis virus) rna. 19734356817
rna-dna covalent bonds between the rna primers and the dna products formed by rna tumor virus dna polymerase.initiation of dna synthesis by endogenous rna primer molecules was studied with three different rna tumor viruses. the influence of the method of virus disruption on the observed rna-dna bonds was ascertained. ether disrupted virions of both murine leukemia virus (mulv) and the b77 strain of avian sarcoma virus (b77 virus) have rc-dc and ra-da covalent linkages between rna primers and newly synthesized dna. none of the 14 other possible bonds were formed. ether-disrupted virions of avian myelobl ...19734357517
integrated state of oncornavirus dna in normal chicken cells and in cells transformed by avian myeloblastosis virus.the covalent linkage of oncornavirus-specific dna to chicken dna was investigated in normal chicken embryo fibroblasts (cef) and in virus-producing leukemic cells transformed by avian myeloblastosis virus (amv). the virus-specific sequences present in cellular dna fractionated by different methods were detected by dna-rna hybridization by using 70s amv rna as a probe. in cef and in leukemic cells, the viral dna appeared to be present only in the nucleus. after cesium chloride-ethidium bromide de ...19734359950
function of top component a rna in the initiation of infection by alfalfa mosaic virus. 19734684580
electric permittivity of alfalfa mosaic virus in aqueous solutions. 19734687139
replication of alfalfa mosaic virus rnas. 19744821703
confirmation of the constitution of alfalfa mosaic virus hybrid genomes by backcross experiments. 19744826212
rna contents of abnormally long particles of certain strains of alfalfa mosaic virus. 19744833532
composition of alfalfa mosaic virus nucleoproteins. 19744844424
limited proteolysis of alfalfa mosaic virus: influence on the structural and biological function of the coat protein. 19744362543
presence in leukemic cells of avian myeloblastosis virus-specific dna sequences absent in normal chicken cells.(3)h-labeled 35s rna from purified avian myeloblastosis virus (amv) was exhaustively hybridized with an excess of normal chicken dna to remove all viral rna sequences which are complementary to dna from uninfected cells. the [(3)h]rna which failed to hybridize was isolated by hydroxylapatite column chromatography which separates dna-rna hybrids from single-stranded [(3)h]rna. the residual rna hybridized to leukemic chicken dna but did not rehybridize with normal chicken dna. this demonstrates co ...19744365719
transfection of chicken embryo cells with dna extracted from avian virus-producing neoplastic cells.dna isolated from avian virus-producing leukemic myeloblasts induced the production of viruses, but not morphological transformation, in cultivated chicken fibroblasts. the recovered virus had the same biological characteristics as the original avian myeloblastosis virus (amv) and produced myeloblastosis and nephroblastomas when injected into chickens. neutralization experiments with chicken anti-amv-bai strain a sera showed an antigenic community between the dna-transfected virus and the origin ...19744369392
on influence of aflatoxin b1 on the physical properties of the avian myeloblastosis virus bai strain a (amv) and on the biosynthesis of viral nucleic acids. 19744371293
effect of chemically modified 70s rna from avian myeloblastosis virus (amv) upon the activity of amv dna polymerase.adenine residues of 70s avian myeloblastosis virus (amv) rna are modified when reacted with chloroacetaldehyde. this modification introduces characteristic fluorescent epsilon-adenosine (epsilona) probes which were used to monitor the reaction. under suitable conditions, modified 70s(epsilona) rna was maintained intact and was inactive as a template for the amv dna polymerase. furthermore, it inhibited the reaction catalyzed by amv polymerase when 70s rna was used as template-primer and had no e ...19744372390
mechanistic independence of avian myeloblastosis virus dna polymerase and ribonuclease h.differential inhibition conditions were established for the dna polymerase and rnase h activities of avian myeloblastosis virus (amv) with ether-disrupted amv and a purified enzyme preparation. the rnase h activity of ether-disrupted amv with (ra)(n).(dt)(n) and (ra)(n).(dt)(11) as substrates was inhibited 80 to 100% by preincubation with naf at a final reaction concentration of 27 to 30 mm. under these conditions, the dna polymerase activity was inhibited only 0 to 20%. similar inhibitions were ...19744372408
detection of naturally occurring antibodies to rna-dependent dna polymerase of murine leukemia virus in kidney eluates of akr mice.specific antibodies to the rna-dependent dna polymerase (reverse transcriptase) of murine type c viruses have been isolated from the renal glomeruli of both leukemic and nonleukemic akr mice where they presumably had been deposited as immune complexes. the antibodies were shown to have sedimentation coefficients of 26s to 28s and 5s to 7s on sucrose rate zonal centrifugation. inactivation with monospecific antisera to various mouse immunoglobulins identified antibodies as being in both immunoglo ...19744129792
structural studies of avian myeloblastosis virus: comparison of polypeptides in virion and core component by dodecyl sulfate-polyacrylamide gel electrophoresis.two different systems of dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) in separate laboratories detected analogous patterns of dye bands in virions of avian myeloblastosis virus (amv). at least 11 of the dye bands co-migrated with the major polypeptides reported in rous sarcoma virus. particles with the morphology of the amv core component, obtained after exposure of amv to the nonionic surfactant sterox sl, contained major polypeptides p12, p27, p60, p64, p91, and p98. the polyp ...19744129794
dna polymerase in virions of a reptilian type c virus.a study was made of the dna polymerase of reptilian type c virus isolated from russell's viper spleen cells. simultaneous detection experiments demonstrated the presence of 70s rna and rna-dependent dna polymerase activity in reptilian type c virions. the endogenous activity was dependent on the addition of all four deoxynucleotide triphosphates and demonstrated an absolute requirement for a divalent cation. the reptilian viral dna polymerase elutes from phosphocellulose at 0.22 m salt. in this ...19744129837
the quaternary structure of alfalfa mosaic virus. 19744137292
the effect of inhibitors of protein biosynthesis on the infectivity of alfalfa mosaic virus: role of coat protein. 19744274500
protection of avian myeloblastosis virus (amv) dna polymerase by substrates against heat inactivation. 197410793699
acquisition of new dna sequences after infection of chicken cells with avian myeloblastosis virus.dna-rna hybridization studies between 70s rna from avian myeloblastosis virus (amv) and an excess of dna from (i) amv-induced leukemic chicken myeloblasts or (ii) a mixture of normal and of congenitally infected k-137 chicken embryos producing avian leukosis viruses revealed the presence of fast- and slow-hybridizing virus-specific dna sequences. however, the leukemic cells contained twice the level of amv-specific dna sequences observed in normal chicken embryonic cells. the fast-reacting seque ...197416789139
hydrodynamic diameters of rna tumor viruses. studies by laser beat frequency light scattering spectroscopy of avian myeloblastosis and rauscher murine leukemia viruses.the diffusion constants of avian myeloblastosis virus (amv) and murine leukemia virus (mulv) (rauscher) suspensions in buffer and in 30% sucrose were determined by laser beat frequency light scattering spectroscopy at a series of temperatures ranging rom 5 to 25 degrees. by the use of the stokes-einstein equation, the following hydrodynamic diameters are calculated at 20 degrees: mulv, 154 plus or minus 3 nm in sucrose and 145 plus or minus 7 nm in buffer; amv, 144 plus or minus 3 nm in sucrose ...1975162827
inhibition of rna-dependent dna polymerase reaction by 6-(p-hydroxyphenylazo)-uracil: a result of drug induced dithiothreitol oxidation.6-(p-hydroxyphenylazo)-uracil (hpura) reduced by dithiothreitol inhibited amv or rlv virion associated exogenous rna-dependent dna polymerase reactions. however, the inhibition was variable from experiment to experiment and was not consistent with the base specificity of hpura seen for inhibition of gram positive dna-dependent dna polymerases. increasing the concentration of dithiothreitol reversed the inhibition. furthermore, at non-toxic concentrations, hpura did not influence the plating effi ...1975166420
studies on characterization of the integration sites of avian rna tumor virus-specific dna.a sequential hybridization procedure is described which allows the integration sites of viral-specific dna to be characterized according to their reassociation kinetics. in addition, this approach enables us to estimate the size of the integrated viral dna. endogenous virus sequences in normal cells appear to be associated with cell sequences reiterated 1200 times, and each integration unit is approximately equal to one 35s rna subunit. in amv-infected cells, the additional amv-specific dna sequ ...1975169002
amv rna transcription in cell-free systems and properties of in vitro chromatin-directed rna synthesis.in this report we have presented evidence that viral sequences in the genome of amv-infected myeloblasts can be transcribed in vitro. the rna products synthesized in either nuclei isolated from these cells or by eukaryotic rna polymerase b from the isolated chromatin contained approximately 1% virus-specific sequences. this result, which is in agreement with the fraction of viral rna in infected cells (garapin et al. 1971), is higher than expected from a random transcription of the genome, and t ...1975169005
polypeptides isolated from ribosome-like structures occluded in avian myeloblastosis virus (amv).the protein composititon of ribosome-like particles isolated from amv was determined by acrylamide gel electrophoresis and by immunological methods. it was established that the protein spectrum of ribosome-like particles differed significantly form the total protein spectrum of amv. the most characteristic protein components of ribosome-like particles had a molecular weight in the range of 70 000--110 000. apart from these proteins, the viral ribosomal particles contained a small amount of prot ...1975169489
quantitative and qualitative differences in dna complementary to avian myeloblastosis virus between normal and leukemic chicken cells.hybridization of avian myeloblastosis virus (amv) rna with dna immobilized on filters or in liquid with a vast dna excess was used to measure the viral specific dna sequences in chicken cells. newly synthesized viral dna (v-dna) appears within an hour after infection of chicken embryo fibroblasts (cef) with avian oncornaviruses. a fraction of newly synthesized v-dna becomes integrated into the cellular genome and the remainder gradually disappears. a covalent linkage between v-dna and cellular d ...1975169823
acquisition of viral dna sequences in target organs of chickens infected with avian myeloblastosis virus.the distribution of oncornavirus dna sequences in various tissues of normal chickens and of chickens with leukemia or kidney tumors induced by avian myeloblastosis virus (amv) was analyzed by dna-rna hybridization using 35s amv rna as a probe. all the tissues from normal chickens which were tested contained the same average cellular concentration of endogenous oncornavirus dna. in contrast, different tissues from lekemic chickens and from chickens bearing kidney tumors contained different concen ...1975170415
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