Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| cytoplasmic inclusions in leaf cells infected with lettuce mosaic virus. | 1969 | 5781866 | |
| an electron microscopic study on the size of lettuce mosaic virus. | 1979 | 549393 | |
| [toxocara canis (larva migrans visceralis) from an ophthalmological point of view (author's transl)]. | visceral larva migrans (toxocara canis) is usually a relatively benign disease which is caused by infective second-stage larvae of the common cosmopolitan ascarid of dogs, characterized chiefly by sustained eosinophilia, pulmonary symptoms and hepatomegaly. its severity varies with the number of larvae in the tissue and the immune or allergic state of the infected individual. the most important aspect of the neurotropic larvae perhaps is its potential as a facilitating agent, for instance, for v ... | 1980 | 7253511 |
| towards a system for the identification and classification of potyviruses. ii. virus particle length, symptomatology, and cytopathology of six distinct viruses. | the mean particle lengths of lettuce mosaic virus (lmv), bean common mosaic virus (bcmv), passionfruit woodiness virus (pwv), potato virus y (pvy), sugarcane mosaic virus (scmv), and one strain of bean yellow mosaic virus (bymv) were all shown to be close to 750 nm. however, the length of two other serologically closely related strains of bymv, variants of pea mosaic virus referred to as pmv and spmv, had significantly longer particles, about 792 and 842 nm. each of the distinct viruses had read ... | 1981 | 18635068 |
| production of monoclonal antibodies to viruses in the potyvirus group: use in radioimmunoassay. | hybridomas were developed that secreted antibodies to soybean mosaic (smv), lettuce mosaic (lmv) viruses, and to maize dwarf mosaic (mdmv) virus, strains ap and b. all hybridomas produced antibodies specific to the homologous virus except for one to lmv, which produced antibodies that reacted at a low level with smv and mdmv. monoclonal antibodies against smv were used in a double-antibody sandwich radioimmunoassay. the assay using only one type of monoclonal antibody lacked sensitivity because, ... | 1984 | 6699619 |
| nucleotide sequence of the 3' terminal region of lettuce mosaic potyvirus rna shows a gln/val dipeptide at the cleavage site between the polymerase and the coat protein. | dna complementary to the 3' terminal 1651 nucleotides of the genome of the common strain of lettuce mosaic virus (lmv-o) has been cloned and sequenced. microsequencing of the n-terminus enabled localization of the coat protein gene in this sequence. it showed also that the lmv coat protein coding region is at the 3' end of the genome, and that the coat protein is processed from a larger protein by cleavage at an unusual q/v dipeptide between the polymerase and the coat protein. this is the first ... | 1991 | 2001176 |
| [testing for the safety, reactogenicity and antigenic properties of a live thermostable mumps vaccine made from the leningrad-3 strain]. | a live mumps vaccine (lmv) from strain leningrad-3 with a new stabilizer ls-18 was tested for reactogenicity and antigenic potency. examinations of vaccinated children for vaccination reactions showed its complete areactogenicity and safety. lmv induced synthesis of virus-neutralizing antibodies in 78-82% of the vaccinees. determinations of the dynamics, intensity and duration of circulation of specific antihemagglutinating, antineuraminidase and virus-neutralizing antibodies demonstrated marked ... | 1991 | 1796587 |
| wild measles virus strain: isolation and identification. | four isolates of measles virus (gag, il, buk and shed) were obtained from suspensions of mononuclear cells from patients at the active stage of the disease. vero cells were used for the virus isolation. all the isolates caused in the infected cell culture the appearance of symplasts of differently sized, star- or spindle-shaped multinuclear cells. the specificity of cytopathic effect was proved by the adsorption of monkey erythrocytes on the surface of cells infected by virus. the isolates were ... | 1992 | 1363990 |
| capsid protein and helper component-proteinase function as potyvirus cell-to-cell movement proteins. | the role of bean common mosaic necrosis potyvirus (bcmnv) and lettuce mosaic potyvirus (lmv) proteins was investigated in terms of their capacity to function as viral movement proteins (mps). using escherichia coli-expressed proteins and microinjection techniques, direct evidence was obtained that both the potyviral capsid protein (cp) and helper component- proteinase (hc-pro) function in this capacity, in that both proteins (a) trafficked from cell to cell, (b) induced an increase in plasmodesm ... | 1997 | 9356340 |
| comparison of the complete nucleotide sequences of two isolates of lettuce mosaic virus differing in their biological properties. | the complete nucleotide sequences of the genomic rnas of the 0 and e isolates of lettuce mosaic potyvirus (lmv) have been determined. these two isolates differ by their behavior towards two lettuce resistance genes and by their seed transmission properties. lmv-0 is unable to induce disease in lettuce carrying either one of the mol1 and mol2 recessive resistance genes, whereas lmv-e is able to induce disease in the same plants. the genomes of these two isolates are 10080 nucleotides (nt) in leng ... | 1997 | 9085548 |
| biological and molecular variability of lettuce mosaic virus isolates. | abstract lettuce mosaic potyvirus (lmv) causes severe disease of commercial lettuce crops. lmv isolates show wide biological variability, particularly in their ability to overcome the resistance genes described in lactuca sativa. for a better understanding of the molecular interaction between lettuce and lmv, biological and molecular characterization of a collection of 10 lmv isolates known to differ in virulence or aggressiveness was performed. the ability of these isolates to overcome the resi ... | 1997 | 18945118 |
| construction of full-length cdna clones of lettuce mosaic virus (lmv) and the effects of intron-insertion on their viability in escherichia coli and on their infectivity to plants. | a full length cdna copy of the genomic rna of lettuce mosaic virus (lmv) was constructed under the control of an enhanced camv 35s promoter and of the nos terminator. this construct was found infectious when inoculated to lettuce plants. the intron ii of the bean nitrite reductase gene was engineered into the lmv fl cdna in order to relieve possible deleterious effects of viral sequences to escherichia coli cells and to evaluate the effects of the presence of the intron on the fl cdna infectivit ... | 1998 | 9930200 |
| properties of a virus causing mosaic and leaf curl disease of celosia argentea l. in nigeria. | a sap transmissible virus, causing mosaic and leaf curl disease of celosia argentea, was isolated at vegetable farms in amuwo odofin, tejuoso, and abule ado, lagos, nigeria. the virus had a restricted host range confined to a few species of the amaranthaceae, chenopodiaceae and solanaceae families. it failed to infect several other species of the aizoaceae, brassicaceae, cucurbitaceae, fabaceae, lamiaceae, malvaceae, poaceae and tiliaceae families. the virus was transmitted in a non-persistent m ... | 1998 | 9842442 |
| nucleotide sequence of the 3' terminal region of the genome of four lettuce mosaic virus isolates from greece and yemen. | lettuce mosaic virus (lmv) is an economically important potyvirus causing a severe disease of commercial lettuce crops. based on molecular data, three phylogenetic groups of isolates have previously been discriminated, reflecting their geographical origin (western europe-california, greece, or yemen). sequence information for the entire coat protein domain was only available for one of the western europe-california phylogenetic group. we have now sequenced the 3' terminal region of the genome lm ... | 1999 | 10486115 |
| potyvirus helper component-proteinase self-interaction in the yeast two-hybrid system and delineation of the interaction domain involved. | using the yeast two-hybrid system, a screen was performed for possible interactions between the proteins encoded by the 5' region of potyviral genomes [p1, helper component-proteinase (hc-pro), and p3]. a positive self-interaction involving hc-pro was detected with lettuce mosaic virus (lmv) and potato virus y (pvy). the possibility of heterologous interaction between the hc-pro of lmv and of pvy was also demonstrated. no interaction involving either the p1 or the p3 proteins was detected. a ser ... | 1999 | 10329571 |
| a century of plant virus management in the salinas valley of california, 'east of eden'. | the mild climate of the salinas valley, ca lends itself well to a diverse agricultural industry. however, the diversity of weeds, crops and insect and fungal vectors also provide favorable conditions for plant virus disease development. this paper considers the incidence and management of several plant viruses that have caused serious epidemics and been significant in the agricultural development of the salinas valley during the 20th century. beet curly top virus (bctv) almost destroyed the newl ... | 2000 | 11137170 |
| synthesis of (-)-strand rna from the 3' untranslated region of plant viral genomes expressed in transgenic plants upon infection with related viruses. | when expressed in transgenic tobacco plants, transgene mrna that includes the 3' untranslated region (3' utr) of lettuce mosaic virus served as template for synthesis of complementary (-)-strand rna following an infection by tobacco etch virus, tobacco vein mottle virus or pepper mottle virus, but not when infected with cucumber mosaic virus. deletion of the 3' utr from the transgene abolished the synthesis of (-)-strand transcripts. similar results were obtained in transgenic tobacco plants exp ... | 2000 | 10725441 |
| effects of green fluorescent protein or beta-glucuronidase tagging on the accumulation and pathogenicity of a resistance-breaking lettuce mosaic virus isolate in susceptible and resistant lettuce cultivars. | the rna genome of a resistance-breaking isolate of lettuce mosaic virus (lmv-e) was engineered to express the jellyfish green fluorescent protein (gfp) or beta-glucuronidase (gus) fused to the helper-component proteinase (hc-pro) to study lmv invasion and spread in susceptible and resistant lettuce cultivars. virus accumulation and movement were monitored by either histochemical gus assays or detection of gfp fluorescence under uv light. the gfp- and gus-tagged viruses spread systemically in the ... | 2000 | 10707357 |
| lettuce mosaic virus pathogenicity determinants in susceptible and tolerant lettuce cultivars map to different regions of the viral genome. | full-length infectious cdna clones were constructed for two isolates (lmv-0 and lmv-e) of lettuce mosaic virus (lmv), a member of the genus potyvirus. these two isolates differ in pathogenicity in susceptible and tolerant-resistant lettuce cultivars. in susceptible plants, lmv-0 induces mild mosaic symptoms, whereas lmv-e induces severe stunting, leaf deformation, and a necrotic mosaic. in plants carrying either of the two probably allelic recessive resistance genes mol1 or mol2, lmv-0 is restri ... | 2001 | 11386376 |
| a universal pcr primer to detect members of the potyviridae and its use to examine the taxonomic status of several members of the family. | a universal primer (sprimer: 5'-ggx aay aay agy ggx caz cc-3', x = a, g, c or t; y = t or c; z = a or g), designed from the consensus sequences that code for the conserved sequence gnnsgqp in the nib region of members of the family potyviridae, was used to amplify by rt-pcr the 3'-terminal genome regions from infected plant samples representing 21 different viruses in the family. sequencing of some of the fragments (c. 1.7 kb) showed that the type strain (attc pv-107) of oat necrotic mottle viru ... | 2001 | 11402861 |
| the arabidopsis eukaryotic initiation factor (iso)4e is dispensable for plant growth but required for susceptibility to potyviruses. | an arabidopsis thaliana line bearing a transposon insertion in the gene coding for the isozyme form of the plant-specific cap-binding protein, eukaryotic initiation factor (iso) 4e (eif (iso) 4e), has been isolated. this mutant line completely lacks both eif(iso)4e mrna and protein, but was found to have a phenotype and fertility indistinguishable from wild-type plants under standard laboratory conditions. in contrast, the amount of the related eif4e protein was found to increase in seedling ext ... | 2002 | 12492835 |
| the virological and clinical significance of mutations in the overlapping envelope and polymerase genes of hepatitis b virus. | the potential for hepatitis b virus (hbv) to alter its genome is considerable. this occurs because the virus utilizes a reverse transcription step in replicating the viral genome. like human immunodeficiency virus, the reverse transcriptase of hbv is error prone and as a consequence of specific selection pressures within a host a population of viral quasispecies emerges. hbv mutants with survival advantages over the wild type virus appear within the selective in vivo environment. some of these v ... | 2002 | 12367644 |
| restoration of replication phenotype of lamivudine-resistant hepatitis b virus mutants by compensatory changes in the "fingers" subdomain of the viral polymerase selected as a consequence of mutations in the overlapping s gene. | the introduction of lamivudine (lmv) for the treatment of chronic hepatitis b infection has been an important advance in the management of this disease. however, the long-term efficacy of lmv may become limited by the emergence of antiviral-resistant hepatitis b virus (hbv) mutants. the two most common lmv-resistant mutants produce changes in the viral polymerase protein (rt) of rtm204i and rtl180m/m204v (previously rtm550i and rtl526m/m550v). a number of studies have demonstrated that these hbv ... | 2002 | 12167344 |
| interaction between potyvirus helper component-proteinase and capsid protein in infected plants. | monoclonal antibodies were raised against helper component-proteinase (hcpro) purified from plants infected with the potyvirus lettuce mosaic virus (lmv). these antibodies were used in a two-site triple antibody sandwich elisa assay together with polyclonal antibodies directed against purified virions. an interaction between hcpro and the viral coat protein (cp) was demonstrated in extracts of lmv-infected leaves, as well as for two other potyviruses, plum pox virus and potato virus y. the cp-hc ... | 2002 | 12075097 |
| molecular and biological characterization of lettuce mosaic virus (lmv) isolates reveals a distinct and widespread type of resistance-breaking isolate: lmv-most. | abstract lettuce mosaic virus (lmv) causes an economically important seedborne and aphid-transmitted disease of lettuce and ornamental crops worldwide. the genetic diversity among 73 lmv isolates was examined based on a 216-nucleotide sequence at the variable region encoding the nib-coat protein junction. three clusters of lmv isolates were distinguished: lmv-yar, lmv-greek, and lmv-row. in the latter cluster, two subgroups of isolates, lmv-common and lmv-most, accounted for a large proportion o ... | 2002 | 18943032 |
| the use of green fluorescent protein-tagged recombinant viruses to test lettuce mosaic virus resistance in lettuce. | abstract seed certification and the use of cultivars containing one of two, probably allelic, recessive genes, mo1(1) and mo1(2), are the principal control methods for lettuce mosaic virus (lmv) in lettuce. although for a few lmv isolates, mo1(2) confers resistance with most isolates, the genes mo1(1) or mo1(2) confer a tolerance, and virus accumulation is readily detected in mo1-carrying plants. this phenotype complicates evaluation of the resistance status, in particular for mo1(1), for which ... | 2002 | 18943090 |
| introduction of a nia proteinase cleavage site between the reporter gene and hc-pro only partially restores the biological properties of gus- or gfp-tagged lmv. | lettuce mosaic virus (lmv) isolates lmv-e and lmv-0 differ in their virulence on lettuce varieties carrying the mo1(2) resistance gene, which reduces viral accumulation and blocks the expression of symptoms after infection with avirulent isolates such as lmv-0. previous work had indicated that reporter genes such as gus or gfp affect the biological properties of recombinant lmv isolates in both susceptible and resistant lettuce varieties when fused to the n-terminus of the viral protein hc-pro. ... | 2003 | 14659562 |
| the eukaryotic translation initiation factor 4e controls lettuce susceptibility to the potyvirus lettuce mosaic virus. | the eif4e and eif(iso)4e cdnas from several genotypes of lettuce (lactuca sativa) that are susceptible, tolerant, or resistant to infection by lettuce mosaic virus (lmv; genus potyvirus) were cloned and sequenced. although ls-eif(iso)4e was monomorphic in sequence, three types of ls-eif4e differed by point sequence variations, and a short in-frame deletion in one of them. the amino acid variations specific to ls-eif4e(1) and ls-eif4e(2) were predicted to be located near the cap recognition pocke ... | 2003 | 12857809 |
| the long-term clinical outcome of 1-year treatment of chronic hepatitis b with lamivudine-5 years observation. | the biochemical and virological outcomes of 19 patients with chronic hepatitis b who had been treated with 100 mg per day of lamivudine (lmv) for 1 year from 1995 to 1996 were evaluated. fourteen patients were followed for 4.5-5 years since the end of the treatment without any further active antiviral treatment. during the treatment, dna levels of hepatitis b virus (hbv) were under the detection limit of a hybridization assay in all the 19 patients. however, ymdd mutants appeared in 5 (26%) pati ... | 2003 | 12957201 |
| multiple resistance phenotypes to lettuce mosaic virus among arabidopsis thaliana accessions. | with the aim to characterize plant and viral factors involved in the molecular interactions between plants and potyviruses, a lettuce mosaic virus (lmv)-arabidopsis thaliana pathosystem was developed. screening of arabidopsis accessions with lmv isolates indicated the existence of a large variability in the outcome of the interaction, allowing the classification of arabidopsis accessions into seven susceptibility groups. using a reverse genetic approach, the genome-linked protein of lmv, a multi ... | 2003 | 12848426 |
| lamivudine and famciclovir resistant hepatitis b virus associated with fatal hepatic failure. | lamivudine (lmv) is the only nucleoside analogue approved for the treatment of chronic hepatitis b (chb). lmv, as with other nucleoside analogues including famciclovir (fcv), suppresses the replication of hepatitis b virus (hbv) by targeting the viral polymerase. however, prolonged antiviral therapy results in the emergence of drug resistance hbv which can contribute to virological breakthroughs and recurrent hepatitis flares. | 2003 | 12727536 |
| biochemical identification of proteasome-associated endonuclease activity in sunflower. | proteasomes have been purified from sunflower hypocotyles. they elute with a molecular mass of 600 kda from gel filtration columns and two-dimensional gel electrophoresis indicates that the complex contains at least 20 different protein subunits. peptide microsequencing revealed the presence of four subunits homologous to subunits beta2, beta6, alpha5 and alpha6 of plant proteasomes. these proteasomes have chymotrypsin-like activity and the highly purified fraction of this complex is associated ... | 2003 | 12535608 |
| activity of aphids associated with lettuce and broccoli in spain and their efficiency as vectors of lettuce mosaic virus. | this research sought to identify the aphid virus vector species associated with lettuce and broccoli crops in spain, and to determine their population dynamics and ability to transmit lettuce mosaic virus (lmv). green tile traps and moericke yellow water-pan traps were used to monitor aphid flights during the spring and autumn growing seasons of 2001. aphid species feeding on lettuce were counted weekly. the transmission efficiencies of lmv were determined for the aphid species caught most frequ ... | 2004 | 15036838 |
| basal core promoter and precore mutations in the hepatitis b virus genome enhance replication efficacy of lamivudine-resistant mutants. | during chronic hepatitis b virus (hbv) infection, mutations in the precore (pc) or basal core promoter (bcp) region affecting hbv e antigen (hbeag) expression occur commonly and represent the predominant virus species in patients with hbeag-negative chronic hepatitis b. the pc mutation (g1896a+c1858t) creates a translational stop codon resulting in absent hbeag expression, whereas bcp mutations (a1762t/g1764a) reduce hbeag expression by transcriptional mechanisms. treatment of chronic hbv infect ... | 2004 | 15280461 |
| a simple and efficient method for testing lettuce mosaic virus resistance in in vitro cultivated lettuce. | the potential of a new in vitro inoculation and propagation method developed for lettuce mosaic virus (lmv) on lettuce (lactuca sativa l.) was evaluated by studying lmv infection on in vitro cultivated seedlings or on newly regenerated plantlets. lettuce cultivars differing by their lmv-resistance status were inoculated with various natural lmv isolates as well as with green fluorescent protein (gfp)-tagged recombinant virus isolates. a good correlation was observed between the known resistance ... | 2004 | 14738978 |
| a naturally occurring recombinant isolate of lettuce mosaic virus. | lmv-common and lmv-most are two seed-borne types of lettuce mosaic virus (lmv), genus potyvirus. lmv-most, but not lmv-common, overcomes the resistance afforded to lettuce by two recessive genes, mo11 and mo12. an rt-pcr-based assay thought to be specific for lmv-most also amplified lmv-tn2, previously typified as lmv-common. the sequence of selected regions along the genome indicated that lmv-tn2 is a natural recombinant between lmv-most and lmv-common isolates, with a putative recombination si ... | 2004 | 14689284 |
| highly reproducible transient transfections for the study of hepatitis b virus replication based on an internal gfp reporter system. | high throughput studies of hepatitis b virus (hbv) replication are limited by the absence of convenient and reliable in vitro systems that can be utilised to examine mutations emerging as a consequence of selective pressures such as drug therapy, immune response and genotypic evolution. we have developed an efficient and reproducible method of transfecting a human hepatoma cell line (huh7) with a replication competent clone of hbv, which utilises an internal reporter of transfection efficiency. ... | 2004 | 15350734 |
| specific detection of lettuce mosaic virus isolates belonging to the "most" type. | lettuce mosaic virus (lmv)-most isolates can infect and are seed-borne in cultivars containing the mo1 gene. a reverse transcription and polymerase chain reaction (rt-pcr)-based test was developed for the specific detection of lmv-most isolates. based on the complete genome sequences of three lmv isolates belonging respectively to the most type, the common type and neither of these two types, three different assays were compared: (i) presence of a diagnostic restriction site in the region of the ... | 2004 | 15350742 |
| occurrence and distribution of lettuce mosaic disease in tehran province from iran. | lettuce mosaic virus (lmv) cucumber mosaic virus (cmv) and tomato spotted wilt virus (tswv) were identified in fields of tehran province. in this study 452 leaf samples were collected from the fields throughout the tehran province during 2002 and 2003. distribution of lettuce mosaic virus (lmv), cucumber mosaic virus (cmv), tomato spotted wilt virus (tswv)and arabis mosaic virus (armv) was determined with das-elisa. percentage of single infection to lmv. cmv or tswv was 20.58, 15.93 and 9.96% re ... | 2004 | 15756832 |
| biological and molecular characterization of lettuce mosaic virus from tehran province in iran. | in this study, lettuce samples having lmv infection symptoms were collected from tehran fields during 2003. samples tested for lmv infection by immuno printing. three positive samples in immuno printing collected and their characteristics were determined. in mechanical inoculation, these isolates produced symptoms on chenopodium quinoa, c. amaranticolor, gomphrena globosa, nicotiana benthamiana, lactuca sativa cv. mantilia and cv. terocadero (which contains the mol1 resistance gene and susceptib ... | 2004 | 15756833 |
| failure of the lamivudine-resistant rtm204i hepatitis b virus mutants to efficiently support hepatitis delta virus secretion. | hepatitis delta virus (hdv) is encapsidated by the envelope proteins of hepatitis b virus (hbv). the major hbv lamivudine (lmv)-resistant mutations in the polymerase gene within the reverse transcriptase (rt) region at rtm204v or rtm204i are associated with changes in the overlapping envelope gene products, in particular, the gene encoding small envelope protein (s) at si195m or sw196l/s/stop. we have demonstrated that the lmv resistance mutations corresponding to sw196l/s inhibited secretion of ... | 2005 | 15858045 |
| hcpro, a multifunctional protein encoded by a plant rna virus, targets the 20s proteasome and affects its enzymic activities. | the proteasome is a multicatalytic complex involved in many cellular processes in eukaryotes, such as protein and rna turnover, cell division, signal transduction, transcription and translation. intracellular pathogens are targets of its enzymic activities, and a number of animal viruses are known to interfere with these activities. the first evidence that a plant virus protein, the helper component-proteinase (hcpro) of lettuce mosaic virus (lmv; genus potyvirus), interferes with the 20s protea ... | 2005 | 16099919 |
| high replicative full-length lamivudine-resistant hepatitis b virus isolated during acute exacerbations. | during chronic hbv infections, exacerbations of disease usually occur without clearly understood mechanisms. in this study, full-length hbv genomes were analyzed from four chronic hepatitis b patients who developed resistance to lamivudine [-2'-deoxy-3'-thiacytidine, lmv] accompanied by acute exacerbation of disease. paired full-length hbv isolates were cloned from the sera of patients prior to lmv treatment and after drug resistant breakthrough isolates emerged with exacerbation. compared to th ... | 2005 | 16121368 |
| molecular mapping of the viral determinants of systemic wilting induced by a lettuce mosaic virus (lmv) isolate in some lettuce cultivars. | the isolate af199 of lettuce mosaic virus (lmv, genus potyvirus) causes local lesions followed by systemic wilting and plant death in the lettuce cultivars ithaca and vanguard 75. analysis of the phenotype of virus chimeras revealed that a region within the p1 protein coding region (nucleotides 112-386 in the viral genome) and/or another one within the ci protein coding region (nucleotides 5496-5855) are sufficient together to cause the lethal wilting in ithaca, but not in vanguard 75. this indi ... | 2005 | 15763148 |
| hepatitis b in children: complexities in management. | chronic hepatitis b virus (hbv) infection by definition is persistence of hepatitis b surface antigen (hbsag) in the serum for > or =6 months. the risk of developing chronic hbv infection ranges from 90% in neonates to <5% in immunocompetent adults. hbv acquired by perinatal infection has a prolonged immune-tolerant phase, characterized by the presence of hepatitis be antigen (hbeag), high hbv-dna and normal alanine aminotransferase (alt) levels. efficient and multi-specific helper and cytotoxic ... | 2005 | 16176431 |
| mutations in surface and polymerase gene of chronic hepatitis b patients with coexisting hbsag and anti-hbs. | to investigate the clinical significance and presence of mutations in the surface (s) and overlapping polymerase gene of hepatitis b patients with coexisting hbsag and anti-hbs. | 2006 | 16830379 |
| mass needle stick injury in children from the western cape. | illegal dumping of contaminated medical waste occurs commonly in south africa. there is little information on the management and outcome of the children exposed to and injured by medical waste. on 15 september 1999, 54 children where involved in a mass exposure incident. 44 presented the same evening and 10 following day. used needles and syringes were discarded on their soccer field. children gave one another injections and played darts with the discarded needles. parents were counselled and bl ... | 2006 | 16291833 |
| resistance to adefovir dipivoxil in lamivudine resistant chronic hepatitis b patients treated with adefovir dipivoxil. | adefovir dipivoxil (adv) is a potent nucleotide analogue against both the wild-type and lamivudine (lmv) resistant hepatitis b virus (hbv). the cumulative incidence of adv resistant mutations in the nucleoside/-tide treatment naive chronic hepatitis b patient (chb) at weeks 48, 96, and 144 was 0, 0.8-3%, and approximately 5.9%, respectively. | 2006 | 16461777 |
| the potyviral virus genome-linked protein vpg forms a ternary complex with the eukaryotic initiation factors eif4e and eif4g and reduces eif4e affinity for a mrna cap analogue. | the virus protein linked to the genome (vpg) of plant potyviruses is a 25-kda protein covalently attached to the genomic rna 5' end. it was previously reported that vpg binds specifically to eif4e, the mrnacap-binding protein of the eukaryotic translation initiation complex. we performed a spectroscopic study of the interactions between lettuce eif4e and vpg from lettuce mosaic virus (lmv). the cap analogue m7gdp and vpg bind to eif4e at two distinct sites with similar affinity (k(d) = 0.3 micro ... | 2006 | 16519694 |
| konjak mosaic virus: the complete nucleotide sequence of the genomic rna and its comparison with other potyviruses. | konjak mosaic virus (komv) belongs to the genus potyvirus, family potyviridae. the complete nucleotide sequence of komv f isolate (komv f) was determined. the genome is 9,544 nucleotides long excluding the 3' terminal poly a tail and encodes a typical potyviral 350-kda polyprotein of 3,087 amino acids. phylogenetic analysis using known potyvirus polyproteins shows that komv constitutes a branch with yam mosaic virus, close to another branch including japanese yam mosaic virus, turnip mosaic viru ... | 2006 | 16538420 |
| hepatitis b e antigen-negative chronic hepatitis b: natural history and treatment. | hepatitis b e antigen (hbeag)-negative chronic hepatitis b evolves in the natural history of chronic hepatitis b virus (hbv) infection linked with selection of nonproducing hbeag but replication-competent hbv mutants, and may have a potentially severe and progressive course. effective suppression of hbv replication is the main therapeutic target. sustained off-therapy responses are rare with treatment of finite duration, except perhaps for interferon-based therapies, which induce such responses ... | 2006 | 16673291 |
| multiple resistance traits control plum pox virus infection in arabidopsis thaliana. | twelve arabidopsis accessions were challenged with plum pox potyvirus (ppv) isolates representative of the four ppv strains. each accession supported local and systemic infection by at least some of the ppv isolates, but high variability was observed in the behavior of the five ppv isolates or the 12 arabidopsis accessions. resistance to local infection or long-distance movement occurred in about 40% of all the accession-isolate combinations analyzed. except for nd-1, all accessions showed resis ... | 2006 | 16673941 |
| [a clinical study of adefovir dipivoxil, made in china, for treatment of hepatitis b e antigen-positive patients with chronic hepatitis b]. | to evaluate the efficacy and safety of a china made adefovir dipivoxil (adv) treatment for hepatitis b e antigen-positive patients with chronic hepatitis b. | 2006 | 17196132 |
| comparison of biological and molecular characterization of iranian lettuce mosaic virus isolates. | lettuce mosaic virus (lmv) is one of the most damaging viruses in lettuce and endive cultivating regions. in order to review the characteristics of different lmv isolates of iran during 2004-2005 samples were collected from lettuce fields in esfahan, ghom, khorasan, khuzestan and tehran provinces. all of the isolates were detected by lmv polyclonal antiserum (as-0155, dsmz germany) in elisa and tipa tests. biological purification was done for the lmv isolates and then they were maintained and pr ... | 2006 | 17390892 |
| combination therapy in liver transplant recipients with hepatitis b virus without hepatitis b immune globulin. | conventional therapy to prevent hbv recurrence in liver transplant (ltx) recipients consists of hepatitis b immune globulin (hbig). the aim of this review is to investigate the safety and efficacy of converting hbig and lam therapy to adv and lam therapy. | 2007 | 17404847 |
| the l80i substitution in the reverse transcriptase domain of the hepatitis b virus polymerase is associated with lamivudine resistance and enhanced viral replication in vitro. | long-term lamivudine (lmv) treatment of chronic hepatitis b almost inevitably engenders viral resistance. mutations that result in the replacement of the methionine at position 204 of the deoxynucleoside triphosphate-binding site of the hepatitis b virus (hbv) reverse transcriptase (rt) by isoleucine, valine, or (rarely) serine (rtm204i/v/s) confer high-level resistance to lmv but reduce replication efficiency. the subsequent selection or coselection of secondary mutations that partially restore ... | 2007 | 17438047 |
| analysis of the serological variability of lettuce mosaic virus using monoclonal antibodies and surface plasmon resonance technology. | a panel of 19 monoclonal antibodies (mabs) was used to study the immunological variability of lettuce mosaic virus (lmv), a member of the genus potyvirus, and to perform a first epitope characterization of this virus. based on their specificity of recognition against a panel of 15 lmv isolates, the mabs could be clustered in seven reactivity groups. surface plasmon resonance analysis indicated the presence, on the lmv particles, of at least five independent recognition/binding regions, correlati ... | 2007 | 17698673 |
| central domain of a potyvirus vpg is involved in the interaction with the host translation initiation factor eif4e and the viral protein hcpro. | using recombinant proteins produced in bacteria or in infected plants, interactions between the vpg and hcpro of lettuce mosaic potyvirus (lmv) and between lmv vpg and the lettuce translation initiation factor 4e, the cap-binding protein (eif4e), were demonstrated in vitro. interaction with eif4e and hcpro both involved the same vpg central domain. the structure of this domain in the vpg context was predicted to include an amphiphilic alpha-helix, with the amino acids related to biological funct ... | 2007 | 17325377 |
| [a clinical study of adefovir dipivoxil treatment for chronic hepatitis patients with cirrhosis in their decompensation period]. | to evaluate the efficacy and safety of 48 weeks adefovir dipivoxil (adv) treatment for chronic hepatitis patients with cirrhosis in their decompensation period. | 2007 | 18073064 |
| [one-step cytometric bead array for rapid detection of potato virus a]. | based on immunoassay, we developed a method to detect potato virus a (pva) using one-step cytometric bead array (cba). | 2008 | 18479067 |
| [a study on mutations of the overlapping hepatitis b virus surface and polymerase gene in patients with hbv reinfection after liver transplantations]. | to investigate the influence of combined hepatitis b immune globulin (hbig) and lamivudine (lmv) treatment on hepatitis b virus (hbv) surface antigen and polymerase overlapping gene mutations in hbv reinfected liver transplant recipients. | 2008 | 18423147 |
| defective hepatitis b virus dna is not associated with disease status but is reduced by polymerase mutations associated with drug resistance. | defective hepatitis b virus dna (ddna) is reverse-transcribed from spliced hepatitis b virus (hbv) pregenomic messenger rna (pgrna) and has been identified in patients with chronic hbv (ch-b). the major 2.2-kb spliced pgrna encodes a novel hbv gene product, the hepatitis b splice protein (hbsp) via a deletion and frame shift within the polymerase. although spliced rna and hbsp expression have been associated with increased hbv dna levels and liver fibrosis, the role of ddna in hbv-associated dis ... | 2008 | 18571815 |
| tenofovir plus lamivudine as rescue therapy for adefovir-resistant chronic hepatitis b in hepatitis b e antigen-positive patients with liver cirrhosis. | there is no consensus on the management of patients with adefovir (adv)-resistant hepatitis b virus (hbv) infection. the aim of this study was to investigate whether tenofovir disoproxil fumarate (tdf) combined with lamivudine (lmv) is effective and safe in patients with resistance to or non-response to adv. | 2008 | 18312291 |
| mutational analysis of plant cap-binding protein eif4e reveals key amino acids involved in biochemical functions and potyvirus infection. | the eukaryotic translation initiation factor 4e (eif4e) (the cap-binding protein) is involved in natural resistance against several potyviruses in plants. in lettuce, the recessive resistance genes mo1(1) and mo1(2) against lettuce mosaic virus (lmv) are alleles coding for forms of eif4e unable, or less effective, to support virus accumulation. a recombinant lmv expressing the eif4e of a susceptible lettuce variety from its genome was able to produce symptoms in mo1(1) or mo1(2) varieties. in or ... | 2008 | 18480444 |
| nitazoxanide, tizoxanide and other thiazolides are potent inhibitors of hepatitis b virus and hepatitis c virus replication. | nitazoxanide (ntz), a thiazolide anti-infective, is active against anaerobic bacteria, protozoa, and a range of viruses in cell culture models, and is currently in phase ii clinical development for treating chronic hepatitis c. in this report, we characterize the activities of ntz and its active metabolite, tizoxanide (tiz), along with other thiazolides against hepatitis b virus (hbv) and hepatitis c virus (hcv) replication in standard antiviral assays. ntz and tiz exhibited potent inhibition of ... | 2008 | 17888524 |
| evidence of long-term suppression of hepatitis b virus dna by tenofovir as rescue treatment in patients coinfected by hiv. | the efficacy of long-term hepatitis b virus (hbv) treatment with tenofovir (tdf) in relation to lamivudine (lmv) resistance in hiv patients failing on lmv deserves further investigations. | 2008 | 18572746 |
| lettuce mosaic virus: from pathogen diversity to host interactors. | taxonomy: lettuce mosaic virus (lmv) belongs to the genus potyvirus (type species potato virus y) in the family potyviridae. physical properties: the virion is filamentous, flexuous with a length of 750 nm and a width of 15 nm. the particles are made of a genomic rna of 10 080 nucleotides, covalently linked to a viral-encoded protein (the vpg) at the 5' end and with a 3' poly a tail, and encapsidated in a single type of capsid protein. the molecular weight of the capsid protein subunit has been ... | 2008 | 18705846 |
| [the efficacy of adefovir dipivoxil monotherapy and the incidence of genotypic resistance to adefovir dipivoxil in patients with lamivudine-resistant chronic hepatitis b infection]. | adefovir dipivoxil (adv) is a nucleotide analogue that inhibits wild-type hepatitis b virus (hbv) and lamivudine (lmv)-resistant hbv mutants. the aim of this study was to elucidate the efficacy of adv monotherapy and the incidence of genotypic resistance to adv in patients with lmv-resistant chronic hbv infection. | 2008 | 19119245 |
| intrinsic disorder in viral proteins genome-linked: experimental and predictive analyses. | vpgs are viral proteins linked to the 5' end of some viral genomes. interactions between several vpgs and eukaryotic translation initiation factors eif4es are critical for plant infection. however, vpgs are not restricted to phytoviruses, being also involved in genome replication and protein translation of several animal viruses. to date, structural data are still limited to small picornaviral vpgs. recently three phytoviral vpgs were shown to be natively unfolded proteins. | 2009 | 19220875 |
| n-terminal of papaya ringspot virus type-w (prsv-w) helper component proteinase (hc-pro) is essential for prsv systemic infection in zucchini. | the papaya ringspot virus (prsv) is one of the limiting factors affecting papaya and cucurbits production worldwide. prsv belongs to the potyvirus genus which consists of 30% of known plant viruses. two serological closely related strains, namely type-p and -w, have been reported. prsv type-p infects both papaya and cucurbits, while type-w infects only cucurbits. the genome of prsv thailand isolate consists of a (+) rna molecule of 10323 nucleotides, which is first translated into a single polyp ... | 2009 | 19322647 |
| the determinant of potyvirus ability to overcome the rtm resistance of arabidopsis thaliana maps to the n-terminal region of the coat protein. | in arabidopsis thaliana columbia (col-0) plants, the restriction of tobacco etch virus (tev) long-distance movement involves at least three dominant rtm (restricted tev movement) genes named rtm1, rtm2, and rtm3. previous work has established that, while the rtm-mediated resistance is also effective against other potyviruses, such as plum pox virus (ppv) and lettuce mosaic virus (lmv), some isolates of these viruses are able to overcome the rtm mechanism. in order to identify the viral determina ... | 2009 | 19737103 |
| [a clinical study of adefovir dipivoxil treatment for chronic hepatitis patients with cirrhosis in their decompensation period]. | to evaluate the efficacy and safety of 96 weeks adefovir dipivoxil (adv) treatment for chronic hepatitis patients with cirrhosis in their decompensation period. | 2009 | 19912686 |
| viral dynamics of hepatitis b virus dna in human immunodeficiency virus-1-hepatitis b virus coinfected individuals: similar effectiveness of lamivudine, tenofovir, or combination therapy. | following treatment of hepatitis b virus (hbv) infection with nucleos(t)ide reverse transcriptase inhibitors (nrtis), there is a biphasic clearance of hbv, similar to that seen following treatment of human immunodeficiency virus-1 (hiv-1) and hepatitis c virus. little is known about the impact of combination nrtis and hiv-1 coinfection on hbv viral kinetic parameters following the initiation of hbv-active highly active antiretroviral therapy (haart). hiv-1-hbv coinfected patients (n = 21) were e ... | 2009 | 19115219 |
| involvement of the cylindrical inclusion (ci) protein in the overcoming of an eif4e-mediated resistance against lettuce mosaic potyvirus. | the capacity of lettuce mosaic virus to overcome the lettuce resistance conferred by the mo1(1) and mo1(2) alleles of the gene for eukaryotic translation initiation factor 4e (eif4e) was analysed using reverse genetics. mutations in the virus genome-linked protein (vpg) allowed mo1(1) only to be overcome, but mutations in the c-terminal portion of the cylindrical inclusion (ci) protein allowed both alleles to be overcome. site-directed mutagenesis pinpointed a key role of the amino acid at posit ... | 2009 | 19161357 |
| successful cessation of lamivudine using interferon in a patient with chronic hepatitis b who received prophylactic lamivudine treatment during chemotherapy. | lamivudine (lmv) prophylaxis is effective in preventing hepatitis b virus (hbv) reactivation in patients with chronic hepatitis b undergoing chemotherapy. however, the optimal duration of lmv prophylaxis remains unclear. we report herein the case of a woman with localized follicular b-cell lymphoma who received chemotherapy with lmv prophylaxis. she achieved complete response to lymphoma, and lmv treatment was continued for 8 months after completion of chemotherapy. hbv status was still inactive ... | 2009 | 26192298 |
| simvastatin potentiates the anti-hepatitis b virus activity of fda-approved nucleoside analogue inhibitors in vitro. | statins are 3-hydroxyl-3-methylglutaryl coenzyme a (hmg coa) reductase inhibitors used for the treatment of hypercholesterolemia. we report that a particular statin, simvastatin (sim), exhibits strong in vitro anti-hbv activity. moreover, a combination of sim with each of the individual nucleos(t)ide analogues lamivudine (lmv), adefovir (adv), tenofovir (ten) and entecavir (etv), showed synergistic antiviral activity. combination drug treatments were performed in the hepg2.2.15 cell line. compou ... | 2010 | 20211652 |
| importance of serum concentration of adefovir for lamivudine-adefovir combination therapy in patients with lamivudine-resistant chronic hepatitis b. | lamivudine (lmv)-adefovir pivoxil (adv) combination therapy suppresses the replication of lmv-resistant hepatitis b virus (hbv), although its efficacy in suppressing hbv varies among patients. this study analyzed the clinical, virological, and pharmaceutical factors that influence the effect of the combination therapy. patients negative for hepatitis b virus e antigen (hbeag) and with low hbv dna titers immediately prior to the combination therapy effectively cleared serum hbv dna (p=0.0348 and ... | 2010 | 20498322 |
| development of mathematical models for the analysis of hepatitis delta virus viral dynamics. | mathematical models have shown to be extremely helpful in understanding the dynamics of different virus diseases, including hepatitis b. hepatitis d virus (hdv) is a satellite virus of the hepatitis b virus (hbv). in the liver, production of new hdv virions depends on the presence of hbv. there are two ways in which hdv can occur in an individual: co-infection and super-infection. co-infection occurs when an individual is simultaneously infected by hbv and hdv, while super-infection occurs in pe ... | 2010 | 20862328 |
| the 20s proteasome α5 subunit of arabidopsis thaliana carries an rnase activity and interacts in planta with the lettuce mosaic potyvirus hcpro protein. | in plants, the ubiquitin/26s proteasome system (ups) plays a central role in protein degradation and is involved in many steps of defence mechanisms, regardless of the types of pathogen targeted. in addition to its proteolytic activities, the ups ribonuclease (rnase) activity, previously detected in 20s proteasome preparations from cauliflower and sunflower (helianthus annuus), has been shown to specifically target plant viral rnas in vitro. in this study, we show that recombinant arabidopsis th ... | 2010 | 21199564 |
| hepatitis b virus-induced liver fibrosis and cirrhosis: the value of liver and spleen volumetry with multi-detector spiral computed tomography. | to study the correlation of liver and spleen volume with the degree of liver fibrosis and cirrhosis induced by hepatitis b virus infection. | 2010 | 20649734 |
| prevalence and clinical characterization of patients with acute hepatitis b induced by lamivudine-resistant strains. | acute hepatitis caused by lamivudine (lmv)-resistant strains has not been reported, and the clinical impact of lmv-resistant strains on acute hepatitis is not known. the aim of this study was to investigate the molecular and clinical characteristics of patients with acute hepatitis b caused by lmv-resistant strains. | 2010 | 20074155 |
| long-term add-on therapy with adefovir in lamivudine-resistant kidney graft recipients with chronic hepatitis b. | to assess the long-term effectiveness and safety of adefovir (adv) plus lamivudine (lmv) in lmv-resistant (r) kidney transplants with chronic hepatitis b, 11 such patients were treated with add-on adv. | 2011 | 21486869 |
| large-scale survey of naturally occurring hbv polymerase mutations associated with anti-hbv drug resistance in untreated patients with chronic hepatitis b. | drug resistance is a major limitation for the long-term efficacy of antiviral therapy with nucleos(t)ide analogues (nas) in chronic hepatitis b (chb). antiviral resistance mutations may pre-exist in the overall viral population of untreated patients. we aimed to assess the prevalence of such hepatitis b virus (hbv) variants in a large cohort of nas-na+»ve patients with chb and to explore possible association with viral and host variables. serum samples from 286 nas-na+»ve consecutive patients wi ... | 2011 | 21692935 |
| aminotransferase serum levels decrease after initiating antiretroviral treatment in hiv infected patients. | to evaluate the effect of antiretroviral treatment on aminotransferase serum levels in treatment-naïve patients infected with human immunodeficiency virus (hiv). | 2011 | 21214507 |
| simultaneous presentation of hemophagocytic syndrome and mesenteric vasculitis in a patient with systemic lupus erythematosus. | we report an 18-year old female patient with systemic lupus erythematosus (sle), who developed fever, pancytopenia, abdominal pain, and watery diarrhea. computed tomography (ct) and bone marrow aspirate revealed lupus mesenteric vasculitis (lmv) and hemophagocytic syndrome (hps). serologic tests for epstein-barr virus (ebv) indicated its reactivation. this case demonstrates that hps and concomitant lmv associated with viral reactivation can occur as clinical manifestations of sle flare. | 2011 | 21229376 |
| Efficacy of Entecavir and Adefovir Combination Therapy for Patients with Lamivudine- and Entecavir-Resistant Chronic Hepatitis B. | BACKGROUND: The optimal treatment of patients with chronic hepatitis B (CHB) who develop resistance to both lamivudine (LMV) and entecavir (ETV) after sequential monotherapy of LMV and ETV remains little known. METHODS: We evaluated the efficacy of entecavir (ETV) plus adefovir dipivoxil (ADV) combination therapy for patients with resistance to LMV and ETV. We reviewed the medical records of 12 patients, and trea ... | 2011 | 22134785 |
| hepatitis b surface antigen seroconversion is associated with favourable long-term clinical outcomes during lamivudine treatment in hbeag-negative chronic hepatitis b patients. | the aims of this study were to assess hepatitis b surface antigen (hbsag) seroconversion and to determine its impact on the natural course of the disease in patients with hbeag-negative chronic hepatitis b (chb) during lamivudine (lmv) treatment. a total of 183 consecutive patients with hbeag-negative chb who were treated with lmv were included in the study. data were retrospectively collected from outpatient visit charts. the primary endpoint was hbsag seroconversion to anti-hbs. the secondary ... | 2011 | 22329377 |
| virologic response is not durable after adefovir discontinuation in lamivudine-resistant chronic hepatitis b patients. | we investigated the durability of the biochemical and virologic responses after adefovir (adv) discontinuation in lamivudine-resistant (lmv-r) chronic hepatitis b (chb) patients, and the outcomes of adv discontinuation compared to that of adv maintenance. | 2011 | 22310790 |
| long-term efficacy of entecavir in adefovir-refractory chronic hepatitis b patients with prior lamivudine resistance. | this study aimed to evaluate the long-term efficacy of entecavir (etv) in adefovir (adv)-refractory chronic hepatitis b (chb) patients with prior lamivudine (lmv) resistance. a total of 55 adv-refractory chb patients with prior lmv resistance, who received rescue therapy with etv 1 mg daily for at least 12 months, were consecutively enrolled and analysed. forty-four patients were men, and their median age was 47 (25-69). ten patients had liver cirrhosis and 46 patients were positive for hepatiti ... | 2011 | 21914066 |
| [evolution of hepatitis b virus quasispecies during antiviral therapy in one chronic hepatitis b patient]. | to investigate the evolution of hepatitis b virus (hbv) quasispecies in one patient during lamivudine (lam) monotherapy and switching to entecavir (etv) rescue treatment. | 2011 | 22152243 |
| dynamics of nonpersistent aphid-borne viruses in lettuce crops covered with uv-absorbing nets. | aphid-transmitted viruses frequently cause severe epidemics in lettuce grown under mediterranean climates. spatio-temporal dynamics of aphid-transmitted viruses and its vector were studied on lettuce (lactuca sativa l.) grown under tunnels covered by two types of nets: a commercial uv-absorbing net (bionet) and a standard net. a group of plants infected by cucumber mosaic virus (cmv, family bromoviridae, genus cucumovirus) and lettuce mosaic virus (lmv, family potyviridae, genus potyvirus) was t ... | 2011 | 22226944 |
| virus diseases in lettuce in the mediterranean basin. | lettuce is frequently attacked by several viruses causing disease epidemics and considerable yield losses along the mediterranean basin. aphids are key pests and the major vectors of plant viruses in lettuce fields. lettuce mosaic virus (lmv) is probably the most important because it is seed-transmitted in addition to be transmissible by many aphid species that alight on the crop. tomato spotted wilt virus (tswv) is another virus that causes severe damage since the introduction of its major vect ... | 2012 | 22682170 |
| genotype-specific mutations in the polymerase gene of hepatitis b virus potentially associated with resistance to oral antiviral therapy. | the evolution of hepatitis b virus (hbv) and the role of different variants during antiviral therapy may be influenced by hbv genotype. we have therefore analysed substitutions potentially related to nucleos(t)ide analogues (nas) resistance at 42 positions within rt-region in a cohort of patients with chronic hepatitis b in relation to hbv-genotype. rt mutations analysis was performed by direct sequencing in 200 nas-naïve patients and in 64 lam or lam+adv experienced patients with nas resistance ... | 2012 | 23026293 |
| Synthesis, stability and pharmacological evaluation of a novel codrug consisting of lamivudine and ursolic acid. | A novel codrug (LMX) was obtained from lamivudine (LMV) and ursolic acid (UA) coupled with ethyl chloroacetate through an amide and ester linkage. The structure of LMX was confirmed by (1)H NMR, (13)C NMR, IR and HRMS. Herein, the in vitro non-enzymatic and enzymatic hydrolysis and in vivo pharmacological activities of LMX were studied. The kinetics of hydrolysis of LMX was studied in aqueous solution of pH 1-10, 80% buffered human plasma and in the presence of lipase from Porcine pancreas (EC 3 ... | 2012 | 22085635 |
| the c terminus of lettuce mosaic potyvirus cylindrical inclusion helicase interacts with the viral vpg and with lettuce translation eukaryotic initiation factor 4e. | recessive resistance to lettuce mosaic virus (lmv) is conferred in lettuce by the mo1 gene, encoding the eukaryotic translation initiation factor 4e (eif4e). the c terminus of the viral cylindrical inclusion helicase (ci-cter), together with the vpg, is involved directly in overcoming mo1 resistance. in this study, recombinant lmv vpg and ci-cter proteins from wild-type or resistance-breaking isolates were expressed and purified from escherichia coli. the allelic forms of eif4e from susceptible ... | 2012 | 21918009 |
| clonal evolution of hepatitis b virus polymerase gene mutations during lamivudine-adefovir combination treatment. | to identify hepatitis b virus polymerase gene mutations during antiviral therapy using lamivudine-adefovir sequential monotherapy followed by lamivudine-adefovir combination therapy. | 2012 | 23197889 |
| the rtm resistance to potyviruses in arabidopsis thaliana: natural variation of the rtm genes and evidence for the implication of additional genes. | the non conventional rtm (restricted tobacco etch virus movement) resistance which restricts long distance movement of some plant viruses in arabidopsis thaliana is still poorly understood. though at least three rtm genes have been identified, their precise role(s) in the process as well as whether other genes are involved needs to be elucidated. | 2012 | 22723957 |
| a case of hepatitis b reactivation due to the hepatitis b virus escape mutant in a patient undergoing chemotherapy. | a 62-year-old man had chronic hepatitis b virus (hbv) infection and was diagnosed with liver cirrhosis. at the time of diagnosis the patient's virologic markers were positive for hepatitis b surface antigen (hbsag), antibody to hepatitis b e antigen (anti-hbe) and antibody to hepatitis b core antigen (anti-hbc), while antibody to hepatitis b surface antigen (anti-hbs) and hbv dna were negative. later the patient received chemotherapy for malignancy. however, this was interrupted due to elevated ... | 2012 | 23180290 |
| dynamics of hepatitis b virus resistance substitutions correlates with virological response in lamivudine-refractory patients with entecavir rescue monotherapy. | entecavir (etv) demonstrates potent antiviral effects against lamivudine (lmv)-resistant hepatitis b virus (hbv). this study was designed to investigate the impact of lmv resistance mutations on the outcome of etv rescue therapy in lmv-refractory patients. twenty-six chronic hepatitis b patients who received etv monotherapy for lmv resistance were enrolled. dynamics of hbv dna levels were monitored before and during etv rescue therapy. mutations in the hbv reverse transcriptase were examined by ... | 2013 | 23968804 |
| a novel method for the analysis of drug-resistant phenotypes of hepatitis b virus. | chronic hepatitis b virus (chb) infection is a major cause of cirrhosis and hepatocellular carcinoma. nucleoside analogs (nas) are popularly used to treat chronic hepatitis b virus (hbv) infections; however, the anti-hbv effect is attenuated by drug-resistant viral mutations selected during long-term antiviral therapy. the timely analysis of drug-resistance mutations is essential in order to adjust treatment regimes. in this study, a t1699c substitution was introduced into the x gene of phbv1.3 ... | 2013 | 23403838 |