Publications

serological relationship of chrysanthemum virus b to carnation latent virus and potato viruses m and s.		1962	13913907
chrysanthemum virus b: antibodies to structural protein in mammals and mg2(+)-dependent reverse-transcriptase activity.	human plasma has been found to contain antibodies reacting with the structural protein of chrysanthemum virus b (cv-b) and about 10 times less intensively with the structural protein of another carlavirus, the potato virus m. it has been shown that the antibodies bind to cv-b through their f(ab)2 fragments. no reaction with proteins of other plant viruses or retroviruses was observed. antibodies reacting with cv-b protein are also present in the plasma of green monkey, goat, rabbit, and mouse, t ...	1989	2483603
nucleotide sequence and gene organization of the 3'-terminal region of chrysanthemum virus b genomic rna.	dna clones complementary to the 3'-terminal 3426 nucleotides of the genomic rna of the carlavirus chrysanthemum virus b (cvb) have been sequenced. the sequence contains six open reading frames (orfs) which encode putative proteins (in the 5'----3' direction) of mr 25,749 (orf2), mr 11,435 (orf3), mr 6984 (orf4), the triple gene block proteins, a protein mr 34,638 (orf5); the coat protein, and a protein of mr 12,609 (orf6). the latter protein is basic and contains a putative zinc finger motif. th ...	1991	1919520
characterization of the 3'-terminal nucleotide sequence of two korean isolates of daphne virus s support its placement as a distinct species of the genus carlavirus.	this study determined the 3'-terminal nucleotide sequences of two korean isolates of daphne virus s (dvs), a tentative member of the genus carlavirus, causing leaf distortion and chlorotic spot disease symptoms in daphne plants. the 3'-terminal 1,465 nucleotide sequences of the two isolates contained two open reading frames coding for proteins of 36 kda viral coat protein (cp) and 12 kda from the 5'-3' end, which is a typical genome structure of the 3'-terminal region of carlaviruses. both dvs i ...	2003	14551815
[interaction of cysteine-rich protein of carlavirus with plant defense system].	viruses of genus carlavirus encode a small cysteine-rich protein (crp) of unknown function. to investigate the role of crp of carlavirus chrysanthemum virus b (cvb), a recombinant potato virus x (pvx) genome was constructed, which carried the cvb crp gene. expression of cvb crp in the pvx genetic background drastically changed the pvx symptom phenotype in n. benthamiana. instead of symptomless infection and mild mosaic, which are characteristic of pvx in this plant host, the recombinant virus ex ...	2005	16240723
genome structure and complete sequence of genomic rna of daphne virus s.	the complete genomic nucleotide sequence and structure of daphne virus s (dvs), a daphne-infecting member of the genus carlavirus, were determined. the genome of dvs was 8,739 nucleotides long, excluding the poly (a) tails. the genome of dvs contained six open reading frames coding for proteins of mr 227 kda (viral replicase), 25 kda, 11 kda and 7 kda (triple gene block tgb) proteins 1, 2 and 3), 35 kda (coat protein; cp), and 12 kda from the 5' to 3' ends; respectively. this is the typical geno ...	2006	16096707
coat protein gene diversity among chrysanthemum virus b isolates from india.	the complete coat protein (cp) sequences from 29 indian isolates of chrysanthemum virus b (cvb) were determined and analysed in relation to other previously characterized carlaviruses. the cp genes of the indian cvb isolates were highly heterogeneous, sharing nucleotide sequence identities of 74-98%. based on phylogenetic analyses, the isolates formed three groups potentially representing either two or three major cvb strain groupings. recombination analysis revealed at least one definite recomb ...	2007	17006596
complete nucleotide sequence of a japanese isolate of chrysanthemum virus b (genus carlavirus).	the complete nucleotide sequence of a chrysanthemum virus b isolate from japan (cvb-s) has been determined. the genomic rna of cvb-s is 8,990 nucleotides long, excluding the poly(a) tail and, like that of other carlaviruses, contains six open reading frames (orfs). multiple alignment and phylogenetic analyses indicated that the phylogenetic relationship among members of the genus carlavirus is very diverse, with phlox virus s being the closest relative of cvb. in aphid transmission tests, cvb-s ...	2007	17726637
role of the zinc-finger and basic motifs of chrysanthemum virus b p12 protein in nucleic acid binding, protein localization and induction of a hypersensitive response upon expression from a viral vector.	the genomes of carlaviruses encode cysteine-rich proteins (crps) of unknown function. the 12 kda crp of chrysanthemum virus b (cvb), p12, has been shown previously to induce a hypersensitive response (hr) when expressed from potato virus x (pvx). this study demonstrated that a p12-induced hr was preceded by induction of a number of genes related to pathogenesis, stress and systemic acquired resistance. p12 localized predominantly to the nucleus. interestingly, it was found that p12 bound both rn ...	2009	19218219
genomic sequence analysis of four new chrysanthemum virus b isolates: evidence of rna recombination.	chrysanthemums worldwide suffer from a high incidence of infection with chrysanthemum virus b (cvb), a member of the genus carlavirus, family betaflexiviridae. three major lineages or strains of this virus have been found in india, but none have been characterized beyond the genetic variation they display in their coat protein genes. here, we describe the analysis of four near-complete genome sequences (from the three lineages) representing the genetic diversity of these strains. ranging in size ...	2011	22179900
intermolecular interactions of chrysanthemum virus b coat protein: implications for capsid assembly.	chrysanthemum virus b coat protein constitutes the viral capsid which, besides other functions, encapsulates and protects the viral nucleic acid. we have demonstrated homotypic interaction of the coat protein subunits, essentially important for dimer formation, which is the first step during capsid assembly in vivo and in vitro. interaction capacity of full length and truncated protein has been investigated and important regions have been identified through protein-protein interaction in yeast a ...	2011	23637512