Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| studies on attenuation of poliovirus. ii. attenuation of poliovirus (type 3, saukett strain) by modified passage in developing chick embryo and chick cell culture. | 1962 | 13980305 | |
| [experiences with a new attenuated poliovirus type 3 usol-d bac developed in czechoslovakia]. | 1964 | 14241623 | |
| differentiation of poliovirus type 3 strains by chromatography on aluminiumhydroxide. | 1964 | 14247357 | |
| properties of a new type 3 attenuated poliovirus. 3. a small scale trial with usol-d bac virus in a closed child community. | 1965 | 14269257 | |
| [experiments on viral excretion and serum differentiation during a poliomyelitis epidemic following oral vaccination with poliovirus type-3]. | 1965 | 14304400 | |
| [autohistioradiographic research on "enterohepatic circulation" of attenuated poliovirus type 3 of sabin labelled with i-131. experimental findings]. | 1965 | 14341456 | |
| [neurovirulence of a new attenuated poliovirus type 3 (usol-d) in a monkey experiment]. | 1965 | 4956397 | |
| [autohistoradiographic investigations of the lymphatic and splenic localization of poliovirus, type 3, attenuated according to sabin's method and labeled with i-131. (experimental research)]. | 1966 | 4286574 | |
| [studies on the neurovirulence of the attenuated poliovirus type 3 (usol-d) in monkey experiments]. | 1966 | 4287917 | |
| [on the neurovirulence test of poliomyelitis-live vaccine. iv. pathomorphology of monkey poliomyelitis through neuropathogenic poliovirus type 3 (saukett strain) after intralumbar injection in comparison to the attenuated poliovirus strains]. | 1966 | 4290952 | |
| studies on characteristics of poliovirus. 3. investigation of d, rct, h and n markers of poliovirus isolated after vaccination with the type 1, chat strain. | 1966 | 4293025 | |
| [a new viral exanthema subitum in 2 cases of infection by poliovirus 3]. | 1966 | 5903755 | |
| [poliomyelitis due to poliovirus 3, with fatal outcome in an infant vaccinated with sabin vaccine]. | 1967 | 4303743 | |
| [a poliomyelitis epidemic caused by poliovirus type 3 in the region of poznan in 1968. preliminary communication]. | 1968 | 4318103 | |
| [autohistioradiographic research on the localization in the central nervous system of poliovirus, type 3, attenuated with the sabin method and labeled with i-131]. | 1969 | 4304483 | |
| antiviral phenoxathiins and their analogs. study of the structure-activity relationships for antiviral activity and the replacement ability in poliovirus type 3 dependent variants. | 1970 | 4318071 | |
| [predominance of poliovirus type 3 in hospitalized children in the isolation center of the hospital jesus of guanabara]. | 1971 | 4328918 | |
| preservation of enteroviruses by freeze-drying. | a method was developed for freeze-dry stabilization of poliovirus type 3. an ultrafiltration procedure was used to remove salts from infected tissue culture fluid, and the virus was freeze-dried after suspension in an alkaline organic buffer. this method was further tested with other picornaviruses including poliovirus types 1 and 2, coxsackieviruses a9, a20, b2, and b5, echovirus 11, and the encephalomyocarditis virus. freeze-dried preparations of the poliovirus could be shipped to distant labo ... | 1971 | 4332042 |
| neutralizing capacity of poliovirus type 3 antibodies tested against 5 different intratypic variants of poliovirus type 3. a comparative study in children immunized with live, inactivated, or both types of poliovirus vaccines. | 1971 | 4332939 | |
| early functions of poliovirus. 3. the effect of guanidine on early functions. | 1971 | 4399254 | |
| use of polyelectrolytes and electron microscopy for detection of viruses from stool. | insoluble polyelectrolytes (pe60) were used for the concentration of viruses from stool specimens, confirming the results of wallis et al. (1969). ten percent suspensions inoculated with poliovirus type 3 were used in these experiments. a small number of stool specimens from patients naturally infected with enteroviruses were also tested. preferential adsorption of viruses to pe60 was maximum at a ph range of 4.5 to 6.0. the elution of the adsorbed viruses was optimal at ph 8.5. other parameters ... | 1972 | 4341968 |
| who collaborative studies on poliovirus type 3 strains isolated during the 1968 poliomyelitis epidemic in poland. | in 1968 in poland an extensive outbreak of poliomyelitis, caused by type 3 poliovirus, began about four months after small vaccine trials with the leon 12a(1)b (sabin) and usol-d bac vaccine strains had been carried out. because of the temporal association, and because the first cases appeared in the province in which the usol-d vaccine trial was carried out, a detailed investigation of the strains isolated from cases in the epidemic was made in four laboratories in an attempt to determine wheth ... | 1972 | 4346582 |
| [possibility of diseases affecting the central nervous system caused by vaccine strains of poliovirus, type 3]. | 1972 | 5037754 | |
| poliovirus type 3 isolation from infants with respiratory diseases. | 1973 | 4359718 | |
| morphogenesis of poliovirus 3. formation of provirion in cell-free extracts. | formation of an apparent virion precursor, the provirion, can be demonstrated in cytoplasmic extracts of poliovirus-infected hela cells. | 1973 | 4358165 |
| defective interfering particles of poliovirus. 3. interference and enrichment. | 1973 | 4354478 | |
| studies on the characteristics of poliovirus type 3. ii. characteristics of "hot" clones. | markers d, ist, ea1(oh)3, rct (at sub- and supraoptimal temperatures) and neurovirulence were determined for clones isolated from two lots (s2 and s3) of vaccines containing poliovirus strain leon 12a1b. changes of markers rct, d and neurovirulence were observed in several clones isolated from s2 vaccine. no changes were observed in ist and ea1(oh)3 markers. | 1976 | 63237 |
| booster effect of sabin poliomyelitis vaccine. | studies were carried out on 86 children aged 1-14 years who had titres of antibodies against poliovirus type 3 lower than 1: 32 and against type 2 higher than 1: 4. after a booster dose of poliomyelitis vaccine type 2+3, seroconversion took place in 44 (90%) of 49 children whose original titre was lower than 1: 4 and the geometrical mean titre increased to 1: 244. of 37 children with low original titres of antibodies against type 3, i.e., 1: 4, 1: 8, or 1: 16, the titre increased fourfold or mor ... | 1976 | 1088514 |
| studies on the characteristics of poliovirus type 3. iii. strain characteristics after passage in man. | the markers d, ist, ea1(oh)3 and rct at sub- and supraoptimal temperatures as well as neurovirulence (pmic) for monkeys was determined for strains isolated from children vaccinated with leon 12a1b vaccine, their contacts and from paralytic cases. the strains were isolated at early and late phases of excretion. the changes concerned mainly rct determined at supraoptimal temperatures, d and pmic markers, especially in strains isolated at the late phase of excretion. the passage through the human a ... | 1976 | 11664 |
| characteristics of poliovirus type 3 strains after passage through monkey central nervous system. | cercopithecus aethiops monkeys were inoculated intracerebrally with sabin's type 3 polivoirus strain, and with strains isolated from subjects vaccinated with leon 12a1b and from their contacts. sample of brain, spinal cord, kidneys, lymph nodes, blood and spinal fluid were taken. from these materials, (except of kidneys) 23 poliovirus strains of type 3 were isolated. genetic markers rct 40.3, rct 41, d and ist were determined for these strains. the changes observed in genetic markers of strains ... | 1977 | 15438 |
| isolation of apparently wild strains of poliovirus type 1 from sewage in the ottawa area. | in the first 4 months of 1974, 140 gauze pad samples of sewage collected in the ottawa area were analysed by the bs-c-1 cell system for the presence of viruses pathogenic for humans. viruses were isolated from 111 (79%) of the samples. of the 72 (65%) isolates identified by serology and electron microscopic examination, 56 (78%) were reoviruses and 16 (22%), enteroviruses. the enterovirus isolates included one coxsackievirus b4, one vaccine strain of poliovirus type 3, nine vaccine strains of po ... | 1977 | 188533 |
| inactivation of polioviruses and coxsackieviruses in surface water. | inactivation rates of polioviruses 1 and 3 and coxsackieviruses a-13 and b-1 were determined in situ in the rio grande in southern new mexico, using membrane dialysis chambers. inactivation of the viruses was exponential, and the rates of inactivation were apparently affected principally by the water temperature. stability of the viruses in river water differed, with poliovirus 1 and coxsackie b-1 being most stable. typically 1-log reductions of infectivity at water temperatures ranging between ... | 1977 | 192146 |
| the role of some viruses in acute pneumonias in adults. | virological and bacteriological investigations were performed in 85 patients with acute pneumonias and virus isolation or serological evidence of virus infection were obtained in 37.6% of the cases. influenza a2 and b viruses were incriminated in 14.1% of the patients; parainfluenza viruses in 7% and adenoviruses in 17.2% of the cases. coxsackie virus was isolated from one patient's blood, and poliovirus 3 was recovered in 3 cases. in 5 cases associated virus infections were detected. | 1977 | 194393 |
| epidermodysplasia verruciformis versus disseminated verrucae planae: is epidermodysplasia verruciformis a generalized infection with wart virus? | recently it has been shown that epidermodysplasia verruciformis is induced by human papilloma/virus different from the papilloma/virus of warts, and that 2 distinct viruses-designated hpv-3 and hp-4--are responsible for it. ten cases of epidermodysplasia verruciformis were found to have been caused by hpv-3. clinically and histologically, as well as in the often depressed cell-mediated immunity they closely resembled long-standing verrucae planae, also caused by hpv-3. contrariwise, in epidermod ... | 1979 | 217936 |
| twenty-one years of follow-up studies of familial epidermodysplasia verruciformis. | 21 years of follow-up study of a family with epidermodysplasia verruciformis (e.v.) have shown that members of one family can be infected with different human papillomaviruses (hpvs), either hpv 3 or hpv 4, and sometimes with both. the clinical picture resembled disseminated flat warts in cases induced by hpv 3, whereas in those caused by hpv 4 there were flat red or red-brownish plaques and depigmented pityriasis versicolor-like lesions. malignancies developed only in family members infected wi ... | 1979 | 220107 |
| genetical analysis on the stability of poliovirus type 3 leon 12a1b. | 1979 | 227906 | |
| pattern of plaquing efficiency at different temperatures (ppet) as a differentiation tool among poliovirus type 3 leon 12a1b progenies and its possible use as a genetic marker. | 1979 | 227907 | |
| characterization of human papillomavirus 3 in warts of a renal allograft patient. | multiple flat wart-like lesions of a renal allograft recipient were shown to contain hpv 3 or a serologically crossreacting virus by indirect immunofluorescence with monospecific animal antisera against hpv [1--5]. the patient's serum revealed virus specific antibodies (immunofluorescence titer 1/80). papillomaviruses were isolated and after in vitro iodination 3 major proteins (mw 70.000, 56.000 and 43.000) were detected by sds polyacryalmide gel electrophoresis. dna was extracted from the wart ... | 1979 | 227966 |
| sequence studies of poliovirus rna. iv. nucleotide sequence complexities of poliovirus type 1, type 2 and two type 1 defective interfering particles rnas, and fingerprint of the poliovirus type 3 genome. | the 32p-labelled genomes of poliovirus type 1, 2 and 3 have been digested with rnase t1 and the products separated by two-dimensional gel electrophoresis. all three fingerprints differ in the separation pattern of the large oligonucleotides. the molar yields of the large rnase t1-resistant oligonucleotides of type 1 and type 2 rna of poliovirus rna are close to one. by comparing the yields of these oligonucleotides to the amount of rna from which they originated, the chain length of type 1 polio ... | 1979 | 230285 |
| uv-induced dna repair synthesis in patients with epidermodysplasia verruciformis. | autoradiographic technique was utilized to measure uv-induced dna repair synthesis in 8 patients with epidermodysplasia verruciformis: 2 infected with human papillomavirus 3 (hpv 3), 4 with hpv 4, and 2 simultaneously with hpv 3 and hpv 4. two cell populations were compared, i.e., epidermal cells derived from light-exposed and covered skin, and peripheral lymphocytes. a control group consisted of 10 healthy subjects. dna repair was found to be normal both in the peripheral lymphocytes and in the ... | 1980 | 6244993 |
| comparative biochemical studies of type 3 poliovirus. | a study of the biochemistry of type 3 poliovirus strains which involves the examination of the virus-coded polypeptides in infected cells and the preparation of oligonucleotide maps is reported. the polypeptide patterns were shown to be a relatively stable property of virus strains and distinguished sabin vaccine strains from wild strains of poliovirus type 3. this approach may be of value in deciding the origin (vaccine or nonvaccine) of field isolates of poliovirus. oligonucleotide maps were f ... | 1980 | 6246264 |
| amyotrophic lateral sclerosis with antecedent poliomyelitis. | histopathological and virological studies were performed on autopsy tissue from a 47-year-old man who had a history of acute poliomyelitis at age 15 years and died after a three-year course of amyotrophic lateral sclerosis (als). the poliovirus serologic tests suggested prior infection with poliovirus type 3 but no ongoing poliovirus infection. the cns showed typical features of als with no inclusion bodies or inflammatory cells. attempts to isolate poliovirus in the cns were unsuccessful and re ... | 1980 | 6247998 |
| poliovirus type 3 keratoconjunctivitis. | 1980 | 6251141 | |
| the preparation of specific immune sera against type 3 poliovirus d-antigen and c-antigen and the demonstration of two c-antigenic components in vaccine strain populations. | animals were immunized with purified d-antigen or c-antigen of type 3 poliovirus to produce specific antisera which were used to analyse the antigenic characteristics of the progeny virus in harvests from poliovirus type 3-infected cells. an examination of the virus progeny present at 24 h p.i. of cells with sabin type 3 vaccine strain virus revealed a large population of particles sedimenting at a slightly lower rate (130s) than infectious virus (155s) in addition to slowly sedimenting (80s) em ... | 1980 | 6161996 |
| immunoassay of poliovirus antigens by single-radial-diffusion: development and characteristics of a sensitive autoradiographic zone size enhancement (ze) technique. | the reactions of polioviruses in single-radial-immunodiffusion (srd) tests were investigated with a view to developing accurate and sensitive antigen assay systems. in direct srd tests, employing high concentrations of immune poliovirus serum in agarose gels, poliovirus d-antigens produced clear reaction zones demonstrated by protein staining. the reactions were type-specific for polioviruses of types 1, 2 and 3 but the tests were of low sensitivity, being applicable only to the assay of virus c ... | 1980 | 6161997 |
| infectious disease patterns in the waorani, an isolated amerindian population. | the waorani indians of eastern ecuador provide a unique opportunity for studying exposure of an isolated human population to various infectious disease agents. using serologic tests to determine antibody prevalence, skin test data, and stool examination for parasites, we have been able to construct a profile of infectious diseases which are endemic, and others which have been introduced into the waorani population. these findings are compared with similar data reported from elsewhere in the amaz ... | 1980 | 7369449 |
| identification of papillomaviruses in butchers' warts. | we have studied the papillomaviruses found in the hand warts of 60 butchers, most of them from 2 distant slaughterhouses. warts differing in morphology and location were studied separately. the viruses were identified by molecular hybridization, restriction enzyme analysis and immunofluorescence. four known human papillomaviruses (hpv-1, hpv-2, hpv-3, hpv-4) were detected and one hitherto unknown papillomavirus was identified in 9 butchers. the dna of the latter virus did not anneal with any of ... | 1981 | 6257792 |
| t cell defect in patients with epidermodysplasia verruciformis due to human papillomavirus type 3 and 5. | most of the patients with epidermodysplasia verruciformis (ev) were anergic to sensitization to dinitrochlorobenzene (dncb) and were shown to have a decreased number of t lymphocytes and reduced lymphocyte pha responsiveness. preserved cell-mediated immunity (cmi) was found only in the abortive cases of ev infected with human papillomavirus type 3 (hpv-3). cmi was impaired to the same extent in patients with ev induced by hpv-3 and hpv-5, and in ev cases with combined infection with both viruses ... | 1981 | 6265297 |
| a hybridoma cell line secreting antibody to poliovirus type 3 d-antigen: detection in virus harvest of two d-antigen populations. | a mouse hybridoma cell line (mo56) secreting igg antibody to poliovirus type 3 d-antigen was obtained by fusion of a mouse myeloma cell line with spleen cells from mice immunized with saukett virus. the monoclonal antibody was specific for saukett virus strains in virus-neutralization and single-radial-diffusion tests. in immunoprecipitation tests the monoclonal antibody reacted with intact infectious virus particles (155s) and with a previously undescribed 70s poliovirus particle with d-antigen ... | 1981 | 6270261 |
| correlation between human papillomavirus (hpv) type and histology of warts. | forty warts from different patients and of different clinical type were examined histologically and virologically. eight lesions were found to be associated with human papillomavirus type 1 (hpv 1), 15 tumors were induced by hpv 2, hpv 3 was detected 4 times, hpv 4 twice, and hpv 6 eleven times. hpv 3, hpv 4, and hpv 6 induced warts revealed a correlation between histology and virus type. they are characterized by the so called "edematous type clear cells". in hpv 3 associated flat warts pycnoti ... | 1982 | 6276473 |
| [human papillomavirus and oncogenesis (author's transl)]. | various types of human papillomavirus (hpv) are responsible for different warts. characteristic of hpv infections is a lowered cell-mediated immunity (cmi), especially defective in cases of epidermodysplasia verruciformis induced by hpv5. the malignant transformation seems to be related with the type of hpv, and not with the grade of cmi defect and duration of the infection. a life-long infection with hpv3 in cases of ev does not induce a malignant transformation in spite of heavily depressed cm ... | 1982 | 6283752 |
| viral pollution of seawater at barcelona. | a virological study of sea water was carried out at barcelona (spain) in june and september 1979. three beaches were sampled in june; the september samples were taken in front of an outlet at 2, 5, 20 and 1,000 m offshore. all of the samples from the 3 beaches contained viruses, at rates varying from 0.12 to 1.72 mpncu/l. vaccinal poliovirus type 3 and coxsackie b5 predominated. for the offshore samples, 55% were positive with rates ranging from 0.15 to 0.36 mpncu/l. vaccinal poliovirus type 3 w ... | 1982 | 6297187 |
| genetic and antigenic variation in type 3 polioviruses: characterization of strains by monoclonal antibodies and t1 oligonucleotide mapping. | considerable genetic and antigenic heterogeneity was detected among a collection of 17 poliovirus type 3 strains isolated between 1939 and 1958 in studies using monoclonal antibodies and by t1 oligonucleotide mapping. heterogeneity was detected even amongst a collection of nine viruses designated saukett and assumed to originate from the same prototype virus. the monoclonal antibodies were found to differ in their strain specificities for poliovirus type 3 strains in virus neutralization or sing ... | 1982 | 6181190 |
| immunochemical studies of polioviruses: identification of immunoreactive virus capsid polypeptides. | investigation of the immunological reactions with individual poliovirus capsid polypeptides of antisera and monoclonal antibodies raised against poliovirus type 3 antigens are described. virus polypeptides were separated by electrophoresis, transferred electrophoretically to nitrocellulose sheets and treated with antibody preparations. antibody binding specifically to the virus polypeptides was then detected by application of 125i-labelled anti-immunoglobulin followed by autoradiography. the tec ... | 1982 | 6185628 |
| the relation between acute persisting spinal paralysis and poliomyelitis vaccine--results of a ten-year enquiry. who consultative group. | most of the 13 countries that participated in this ten-year study of the incidence of acute persisting spinal paralysis (apsp) used trivalent live poliomyelitis vaccine (sabin strains), but monovalent vaccines were used for all or part of the time in 3 countries and inactivated vaccines were used wholly by 2 countries and in part by 2 other countries. altogether 698 cases of apsp were recorded in a total population of about 509 million over the 10-year study period - an incidence of 0.14 per mil ... | 1982 | 6980734 |
| problems in the disinfection of class 1 microbiology safety cabinets. | microbiology safety cabinet disinfection procedures using formaldehyde have been tested. tubercle bacilli were killed by concentrations of formaldehyde obtained by heating commercial formalin irrespective of whether the bacilli were in the cabinet free space or above the prefilters. however, bacillus stearothermophilus spore papers for for the testing of low temperature steam/formaldehyde sterilisers were almost never sterilised and a strain of staphylococcus epidermidis (nctc 7944) showed a res ... | 1982 | 7047573 |
| monoclonal antibodies specific for the sabin vaccine strain of poliovirus 3. | monoclonal antibodies to poliovirus type 3 secreted by nine mouse hybridoma cell lines were tested for virus-strain specificity with a collection of wild and vaccine-like type 3 viruses, the genetic characteristics of which had been determined by t1-oligonucleotide mapping. four of the monoclonal antibodies had both virus-neutralising and antigen-blocking activity, and five had antigen-blocking activity only. of the four virus-neutralising antibodies, two (nibp138, nibp165) reacted only with sab ... | 1982 | 6123838 |
| location and primary structure of a major antigenic site for poliovirus neutralization. | we have determined a major antigenic site for virus neutralization on the capsid protein vp1 of poliovirus type 3. antigenic mutant viruses selected for resistance to individual monoclonal antibodies had point mutations concentrated in a region 277-294 bases downstream from the start of the region of viral rna coding for vp1. these findings provide the basis for an improved understanding of the molecular basis of virus neutralization. | 1983 | 6186919 |
| critical role of an eight-amino acid sequence of vp1 in neutralization of poliovirus type 3. | the three serotypes of poliovirus are members of the picornaviradae, a group of viruses which cause a variety of diseases of considerable importance in man and animals. we have previously used antigenic mutants resistant to neutralizing monoclonal antibodies to identify a single antigenic site for the neutralization of poliovirus type 3 (ref. 1). evidence based on oligonucleotide mapping suggested that this site corresponded largely to one physicochemical region of the capsid protein viral polyp ... | 1983 | 6192344 |
| introduction of cloned human papillomavirus genomes into mouse cells and expression at the rna level. | the entire dna genomes of five different human papillomaviruses (hpvs) were cloned into the bamhi site of pbr322 (hpv-1a, hpv-3, hpv-4, and hpv-9) or the ecori site of pbr325 (hpv-2), using as starting materials virus preparations isolated from papillomas of individual patients. under stringent hybridization conditions (tm-28 degrees), the five cloned hpvs exhibited less than 10% homology with one another. to establish model cell systems that may be useful for the identification of hpv genes and ... | 1983 | 6193636 |
| poliovirus type 3: molecular cloning of the genome and nucleotide sequence of the region encoding the protease and polymerase proteins. | overlapping cdna clones representing the entire genome of poliovirus type 3 have been prepared in e. coli by two separate methods. cloning of rna . cdna hybrids produced a more comprehensive set of clones with generally larger cdna inserts than cloning of double - stranded cdna. a restriction map of the entire genome and the nucleotide sequence of 2003 bases from the 3' terminus, comprising the region encoding the protease and polymerase proteins, are presented. | 1983 | 6298739 |
| characterization of two hpv-3 related papillomaviruses from common warts that are distinct clinically from flat warts or epidermodysplasia verruciformis. | we have recently identified two unusual human papillomavirus (hpv) isolates while engaged in an ongoing study of wart disease in meat handlers and veterinarians. the papillomas from which these two viruses were isolated clinically resembled verruca vulgaris rather than either flat warts or epidermodysplasia verruciformis (ev). these two previously uncharacterized hpvs were molecularly cloned and characterized with respect to known hpvs. the genomes of the two viruses exhibited dramatically diffe ... | 1983 | 6302170 |
| the nucleotide sequence of poliovirus type 3 leon 12 a1b: comparison with poliovirus type 1. | the complete nucleotide sequence of the genome of the sabin vaccine strain of poliovirus type 3 (p3/leon 12 a1 b) has been determined from cdna cloned in e. coli. the genome comprises a 5' non-coding region of 742 nucleotides, a large open reading frame of 6618 nucleotides (89% of the sequence) and a 3' non-coding region of 72 nucleotides. there is 77.4% base-sequence homology and 89.6% predicted amino-acid homology between types 1 and 3. conservation of all glutamine-glycine and tyrosine-glycin ... | 1983 | 6310508 |
| nucleic acid sequence of the region of the genome encoding capsid protein vp1 of neurovirulent and attenuated type 3 polioviruses. | three closely related strains of poliovirus type 3 have been used to study the molecular basis of attenuation in the currently used sabin vaccine of this serotype. plaque-purified derivatives of these strains possess closely similar serological and biochemical properties yet differ markedly in neurovirulence for monkeys. molecular cloning via an rna . cdna method has facilitated comparative nucleotide sequencing. initial efforts have concentrated on the region of the genome encoding vp1. only mi ... | 1983 | 6311539 |
| identification of human papillomavirus dna in cervical and vaginal intraepithelial neoplasia with molecularly cloned virus-specific dna probes. | the presence of human papillomavirus (hpv) dna was identified in the tissues of cervical and vaginal intraepithelial neoplasia by southern blot dna hybridization under conditions of low stringency. the specific types of hpv present in the tissues were identified by using molecularly cloned types 1 through 6 (hpv-1 through hpv-6) hpv dna probes under high-stringency conditions. all tissues of cervical and vaginal intraepithelial neoplasia analyzed contained hpv genomes. fifteen of 19 samples (79% ... | 1983 | 6313534 |
| susceptibility of itinerants ("travelling people") in scotland to poliomyelitis. | a total of 109 travelling people (people with a nomadic lifestyle), living on 8 separate sites in central and south scotland, were examined for neutralizing antibodies to poliovirus types 1, 2, and 3. of those studied, 10% had no detectable antibody to poliovirus type 3 while 7% had no antibody to type 1, the types most commonly associated with outbreaks of paralytic poliomyelitis. only one subject, a child aged 9 years, had no detectable poliomyelitis antibody; he had no history of poliomyeliti ... | 1983 | 6317214 |
| molecular cloning and characterization of a unique type of human papillomavirus from an immune deficient patient. | several papillomas from a single patient who exhibited an unusual immune deficiency syndrome were analyzed for the presence of specific human papillomavirus (hpv) types. preliminary analysis indicated that the hpv dna species present in each of these tissues was quite unlike any of the previously characterized hpv types. in order to more rigorously analyze the hpv from this patient we have isolated the hpv dna by molecularly cloning it into a bacteriophage lambda vector and have constructed a de ... | 1984 | 6323588 |
| construction and properties of intertypic poliovirus recombinants: first approximation mapping of the major determinants of neurovirulence. | an attempt was made to map, in a general way, the region of the poliovirus genome that is responsible for the neurovirulent and attenuated phenotypes of different virus strains. a set of four recombinants was investigated, one described previously (e. a. tolskaya, l. i. romanova, m. s. kolesnikova, and v. i. agol, 1983, virology 124, 121-132) and three obtained in the present work with the following genetic structure: a 5' end-adjacent segment of the genome derived from either a virulent strain ... | 1984 | 6330995 |
| molecular cloning of the genomes of poliovirus type 3 strains by the cdna: rna hybrid method. | the genomes of two neurovirulent strains of poliovirus type 3, wild type p3/leon/37 and a vaccine revertant p3/119/70, have been cloned in e. coli. the cdna: rna hybrid method used was efficient and may have wide applicability for cdna cloning. overlapping clones spanning the entire genome were obtained for each strain. these have been used to produce full-length dna copies of the two genomes each within a single plasmid. | 1984 | 6331350 |
| new poliovirus vaccines: a molecular approach. | this article summarizes recent work on the determinants of antigenicity in poliovirus type 3 and reports on experiments in progress aimed at understanding the molecular basis of attenuation in sabin's type 3 vaccines. ways in which this new information might be used to produce alternative, safe, inexpensive, multivalent vaccines against polio and other enteroviruses are discussed. | 1984 | 6085198 |
| reversion to neurovirulence of the live-attenuated sabin type 3 oral poliovirus vaccine. | the complete nucleotide sequence has been determined of a strain of poliovirus type 3, p3/119, isolated from the central nervous system of a victim of fatal vaccine-associated poliomyelitis. comparison of this sequence with those obtained previously for the sabin type 3 vaccine, p3/leon 12a1b and its neurovirulent progenitor, p3/leon/37, reveals that these three strains are on a direct geneaological lineage and therefore that p3/119 is a bona fide revertant of the vaccine. p3/119 differs in sequ ... | 1984 | 6093054 |
| antigenic analysis of poliovirus type 3 using monoclonal antibodies. | hybridoma cell lines secreting monoclonal antibodies to type 3 poliovirus have been prepared and their reactivity with infectious virus ('d' antigen), empty particles ('c' antigen) and isolated virus capsid proteins examined. eight antibodies reacted with epitopes common to 'd' and 'c' antigen and all of these possessed high titres of neutralizing activity. however only 12 out of 19 antibodies which reacted exclusively with 'd' antigen neutralized virus infectivity and some of these reacted only ... | 1984 | 6098495 |
| neutralization epitopes on poliovirus type 3 particles: an analysis using monoclonal antibodies. | monoclonal antibodies to poliovirus type 3 secreted by 51 hybridoma cell clones have been characterized in terms of (i) virus-neutralizing properties, (ii) reactivity in antigen-blocking tests with infectious, 155s ('d' antigen) and empty 80s ('c' antigen) poliovirus particles and (iii) reactivity in immunoblot tests with the isolated protein components of the poliovirus capsid. the antibodies could be separated into three groups on the basis of their reactivities with 'd' and 'c' antigens. all ... | 1984 | 6198447 |
| antigenic characterization of poliovirus type 3 using monoclonal antibodies. | hybridoma cell lines secreting monoclonal antibodies to type 3 poliovirus were prepared, and their reactivity with infectious virus (d antigen), empty particles (c antigen), and isolated virion capsid proteins ( vps ) were examined. eight antibodies reacted with epitopes common to d and c antigens, and all of these possessed high titers of neutralizing activity. however, only 12 of 19 antibodies that reacted exclusively with d antigen neutralized virus infectivity, and some of these reacted only ... | 1984 | 6204368 |
| principal and subsidiary antigenic sites of vp1 involved in the neutralization of poliovirus type 3. | the characterization of over 300 mutants, derived from two strains of poliovirus type 3 and selected for resistance to neutralization by monoclonal antibodies, has led to the further definition of the major antigenic site involved in neutralization. the site encompasses amino acids 89 to 100 of vp1. a subsidiary antigenic site near the c-terminus of vp1 has been identified for the sabin vaccine strain of poliovirus type 3. of 59 monoclonal antibodies to poliovirus type 3 examined, 27 had virus-n ... | 1985 | 2582084 |
| induction by synthetic peptides of broadly reactive, type-specific neutralizing antibody to poliovirus type 3. | a region of virus capsid protein vp1 located 89-100 amino acids from the n-terminus has been proposed to comprise a major antigenic site involved in the neutralization of poliovirus type 3. synthetic peptides 10-18 amino acids in length, containing all or part of this sequence, were tested for their ability to induce antiviral antibodies. rabbits, but not guinea pigs or mice, immunized with the most active peptide, developed hightitered, type-specific, neutralizing antibodies for a wide range of ... | 1985 | 2414909 |
| increased neurovirulence associated with a single nucleotide change in a noncoding region of the sabin type 3 poliovaccine genome. | most of the small number of cases of poliomyelitis which occur in countries where sabin's attenuated poliovirus vaccines are used are temporally associated with administration of vaccine and involve polioviruses of types 2 and 3 (ref. 1). recent studies have provided convincing evidence that the sabin type 2 and 3 viruses themselves may revert to a neurovirulent phenotype on passage in man. we report here that a point mutation in the 5' noncoding region of the genome of the poliovirus type 3 vac ... | 1985 | 2986004 |
| a comparison of the effects of cytotoxic cerebrospinal fluid on cell cultures with other cytopathogenic agents. | protein synthesis, antigen synthesis, and cell membrane permeability were analyzed after inoculating human diploid fibroblasts with control or cytotoxic csf, herpes simplex virus type 1 (hsv 1), poliovirus 3, or clostridium difficile toxin. whereas protein synthesis and membrane permeability were affected by the viruses, and virus antigens detectable by pooled human serum were synthesized, the bacterial toxin and cytotoxic csf did not induce any new proteins or antigens, although the cytotoxic c ... | 1985 | 2987025 |
| x-linked inheritance of epidermodysplasia verruciformis. genetic and virologic studies of a kindred. | we describe a family with typical epidermodysplasia verruciformis (ev) in which only male members are affected. whereas none of the index patient's ten children have ev, four of eight grandsons born to his daughters have inherited the disorder. all are infected with human papillomavirus (hpv) 3 and hpv 8. the inheritance in this kindred most likely results from an x-linked recessive genetic defect. since other kindreds have been described with autosomal inheritance, this novel inheritance patter ... | 1985 | 2990354 |
| recombinants between attenuated and virulent strains of poliovirus type 1: derivation and characterization of recombinants with centrally located crossover points. | recombinants with a centrally located crossover point were selected from crosses between poliovirus type 1 strains and intertypic (type 3/type 1) recombinants. two such recombinants were characterized in some detail. in one of them (v1/a1-6), the 5' half of the genome was derived from a virulent type 1 strain, while the 3' half came from an attenuated type 1 strain. the genome of the other recombinant (a1/v1-7) had the reverse organization, with the 5' and 3' halves being derived from the type 1 ... | 1985 | 2998022 |
| [characteristics of the genome of poliovirus type 3 isolated from patients with poliomyelitis in moldavia]. | twelve poliovirus isolates of serotype 3 from patients with paralytic poliomyelitis have been analyzed by oligonucleotide mapping of the viral genomes. all the studied strains were isolated from patients in different regions of the moldavian ssr in 1982. the maps of all isolates are similar but they do not practically possess any large oligonucleotides characteristic of the vaccine strain of type 3 poliovirus. it is concluded that a wild neurovirulent strain of type 3 poliovirus, that circulated ... | 1985 | 3025721 |
| human papillomavirus infections in a group of renal transplant recipients. | one hundred and twenty renal transplant recipients were investigated. fifty-eight (48%) were found to have warts, 13 (11%) keratoses and six (5%) to have, or recently to have had cancers. the longer the time of immunosuppression, the greater the prevalence of warts; of those patients who had had their transplant for at least 5 years, 87% had warts. those with a graft survival time of 10 years or more are at special risk of warts, keratoses and malignancy. five (10%) of 50 women had genital warts ... | 1986 | 3026431 |
| elimination of human enteric viruses during conventional waste water treatment by activated sludge. | the present study was undertaken to determine if viruses were selectively eliminated during waste water treatment. human enteric viruses were detected at all steps of treatment in a conventional activated sludge waste water treatment plant. liquid overlays and large volume sampling with multiple passages on bgm cells permitted the detection of poliovirus (serotypes 1, 2, and 3), coxsackievirus b (serotypes 1, 2, 3, 4, and 5), and echovirus (serotypes 3, 14, and 22), as well as reoviruses. the me ... | 1986 | 3028589 |
| outbreak of poliomyelitis in finland in 1984. description of nine cases with persisting paralysis. | finland has been free of poliomyelitis since 1964 following a high coverage regular immunization programme using the salk-type trivalent inactivated poliovirus vaccine. in late 1984 an outbreak of poliomyelitis with widespread circulation of poliovirus type 3 throughout the country was found. a thorough surveillance revealed 9 sporadic cases with acute persisting paralysis, all shown to be caused by poliovirus type 3. the preceding 20-year period free from poliomyelitis contributed to some diagn ... | 1986 | 3008307 |
| neurologic complications in oral polio vaccine recipients. | between april 1982 and june 1983 four children 3 to 24 months of age were referred for evaluation of neurologic abnormalities found to be compatible with vaccine-related poliovirus infection, which had not been suspected by referring physicians. patients were epidemiologically unrelated residents of indiana, and none had prior symptoms suggestive of immunodeficiency. all had received poliovirus vaccine orally (first dose in three, fourth dose in one) and a diphtheria-tetanus-pertussis injection ... | 1986 | 3012055 |
| papillomavirus dna in warts of immunosuppressed renal allograft recipients. | renal allograft recipients were shown to have an increased incidence of warts and skin cancers. we examined 148 patients for evidence of wart virus infections and tested for papillomavirus types, which are known to be associated with human malignancies. of the 148, 36 (24.3%) patients were afflicted with warts at the end of our study period, in contrast to 5 of 148 (3.3%) before transplantation. dna from 16 different biopsies was extracted by phenol treatment for further virological studies. dna ... | 1986 | 3015050 |
| modulation of humoral response to a 12-amino-acid site on the poliovirus virion. | most monoclonal antibodies to poliovirus 3 but not poliovirus 1 require a single 12-amino-acid sequence in virion protein vp1 for neutralization (site 1). none of the available monoclonal antibodies requiring this site bound virions after tryptic cleavage of site 1. this result allowed the amount of site 1-specific antibodies to be determined in an antiserum by comparing its reactivity with virus and trypsin-cleaved virus. antisera to poliovirus 3 sabin strain (ps3) but not poliovirus 1 sabin sh ... | 1986 | 3018295 |
| the nucleotide sequence of a type 3 poliovirus isolated during a recent outbreak of poliomyelitis in finland. | we have cloned and sequenced the complete genome of a strain of poliovirus type 3 (23127) isolated during an outbreak of poliomyelitis in finland. the genome is 7435 nucleotides long excluding the 3' poly(a) stretch and is 95.5% homologous at the amino acid level to the previously sequenced type 3 strain, p3/leon/37. the most striking feature of the presented sequence is the extent of amino acid substitutions relative to p3/leon/37 and other type 3 strains in areas of known antigenic importance. ... | 1986 | 3020156 |
| prevalence of antibody to poliovirus in england and wales 1984-6. | serum from 995 subjects aged 6 months to 99 years was screened at a dilution of 1/8 for neutralising antibodies to poliovirus. of these samples, 975 (98%) contained antibody to at least one serotype, and 763 (77%) contained antibody to all three, an improvement since previous studies. children aged 8 to 15, however, had a low prevalence of antibody to poliovirus type 3, with only four (40%) of those aged 12 protected. this finding is possibly due to the waning of antibodies induced by the type 3 ... | 1986 | 3021273 |
| detection of poliovirus antigen by enzyme immunoassay. | a solid-phase enzyme immunoassay (eia) was developed for the detection of poliovirus antigen. rabbit and guinea pig antisera for the assay were raised against purified poliovirus type 3/fin (strain 3/fin/k) isolated from a fecal specimen from a meningitis patient during an outbreak of poliomyelitis in finland in 1984. the eia was highly specific for poliovirus type 3, and it was about 30 times more sensitive for strain 3/fin/k than for strain 3/saukett used in the inactivated poliovirus vaccine. ... | 1986 | 3023440 |
| antigenic and molecular evolution of the vaccine strain of type 3 poliovirus during the period of excretion by a primary vaccinee. | a 4 month old child was immunized with a vaccine containing the sabin live attenuated vaccine strains of all three serotypes of poliovirus. the antigenic and molecular evolution of the sabin strain of poliovirus type 3 was then followed throughout the entire period of virus excretion. novel strains appeared at 8, 42 and 52 days post-vaccination and were the products of both intertypic recombination between type 2 and type 3 poliovirus in regions of the genome coding for non-structural proteins a ... | 1986 | 2420925 |
| reactivity of anti-peptide and anti-poliovirus type 3 monoclonal antibodies with synthetic peptides. | monoclonal antibodies were prepared from mice immunized with an 18-residue synthetic peptide with an amino acid sequence from a major antigenic sequence involved in the neutralization of type 3 poliovirus. approximately 250 hybridomas secreted antibodies that reacted with the peptide but not the virus, two antibodies reacted with the virus but not the peptide and no antibody reacted with both. conversely 26 monoclonal antibodies prepared from mice immunized with type 3 poliovirus and known to be ... | 1986 | 2431104 |
| demonstration of calicivirus in human faeces by immunosorbent and immunogold-labelling electron microscopy methods. | immunosorbent electron microscopy (isem) and immuno-gold staining (igs) electron microscopy methods have been applied to human faeces, shown by direct electron microscopy (em) to contain calicivirus. caliciviruses were successfully trapped on grids coated with positive rabbit or human antisera against calicivirus, but not with negative sera. caliciviruses were specifically labelled with gold particles, when treated with positive rabbit or human antisera against calicivirus followed by protein a- ... | 1986 | 3539960 |
| outbreak of paralytic poliomyelitis in finland: widespread circulation of antigenically altered poliovirus type 3 in a vaccinated population. | an outbreak of 9 cases of paralytic poliomyelitis and 1 non-paralytic case occurred in finland between august, 1984, and january, 1985, after two decades of freedom from the disease attributable to a successful immunisation programme. during the outbreak poliovirus type 3 was isolated from the patients, from about 15% of healthy persons tested, and from sewage water. at least 100 000 persons were estimated to have been infected. with 1.5 million extra doses of inactivated poliovirus vaccine to c ... | 1986 | 2872526 |
| poliovirus antibody in northern greece. | in order to study the serological status of the northern greek population to poliovirus, 881 sera from healthy people were examined for neutralizing antibody by the micrometabolic inhibition test. the people under examination were aged from 1 day to 70 years old. overall, of the 881 sera examined, 704 (80%) had antibodies (titre greater than or equal to 4) to poliovirus 1, 742 (84%) had antibodies to poliovirus 2 and 715 (81%) had antibodies to poliovirus 3. fifty-five per cent of the sera had a ... | 1986 | 3016076 |
| proportions of immunoglobulin isotypes in paralytic poliomyelitis and after vaccination. | immunoglobulin isotype composition of poliovirus antibodies was studied by isotype-specific solid-phase radio-immunoassay (ria) in four patients with paralytic poliomyelitis, five adults receiving live poliovirus vaccine as a booster immunization, and seven children receiving first doses of inactivated poliovirus vaccine. in paralytic poliomyelitis serum and cerebrospinal fluid (csf) poliovirus antibodies were mainly of igg1, igg3, and iga isotypes. igm antibodies were found in sera but not in c ... | 1987 | 3611298 |
| evolution of poliovirus during an outbreak: sequential type 3 poliovirus isolates from several persons show shifts of neutralization determinants. | an outbreak of poliomyelitis in finland resulted in the widespread circulation of wild-type 3 poliovirus strains that had antigenic properties distinct from the strains used to produce the attenuated and inactivated vaccines. considerable variation was observed in the ability of broadly reacting monoclonal antibodies directed against type 3 poliovirus to neutralize the 54 strains examined. sequential isolates from several persons showed an antigenic drift with these monoclonal antibodies and sel ... | 1987 | 2437244 |
| nucleic acid sequence relationships between enterovirus serotypes. | forty-eight different enterovirus serotypes were analysed by a nucleic-acid hybridization test using probes derived from the 3' end of coxsackievirus a21 (ca21) and b3 (cb3), poliovirus 3 (p3) and enterovirus 70 (e70). more than 90% of the serotypes could be detected with this collection of reagents. the cb3 probe reacted with all the coxsackie b viruses, with all three poliovirus serotypes, and with almost all of the 30 echo virus types tested. in addition some of the coxsackie a viruses and th ... | 1987 | 2456458 |
| immune status of the population to poliovirus infection in singapore. | a seroepidemiological survey conducted after two decades of mass immunization against poliomyelitis in singapore showed a high level of herd immunity in the population to all three types of human poliovirus. neutralizing antibodies to poliovirus 1, 2 and 3 were detected in 93.9-96.7% of the population between 6 months and over 40 years old. adults above 30 years of age had a relatively high level of susceptibility to poliovirus infection, with 10.2-12% in the over-30-year age group possessing no ... | 1987 | 3034442 |
| comparison between indirect immunofluorescence and microneutralization for detection of antibodies to polioviruses. | indirect immunofluorescence and microneutralization methods for the detection of antibodies to poliovirus serotypes 1, 2, and 3 were compared. of the 41 sera tested for poliovirus type 1 antibody, 40% were in complete agreement, 55% differed by one dilution, and 5% differed by two dilutions. for poliovirus type 2, 37 sera were tested; 56% completely agreed, and 44% differed by only one dilution. for poliovirus type 3, complete agreement occurred in 59% of 33 sera, while 41% differed by one dilut ... | 1987 | 3038952 |