Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| three-segment rna genome of lumbo virus (bunyavirus). | 1973 | 4210363 | |
| bunyaviruses and bunyaviridae. | 1974 | 4850836 | |
| mosquito-borne arboviruses in arctic america. | mosquito-borne arboviruses are prevalent throughout subarctic regions of canada and alaska, principally in the boreal forest extending between latitudes 53 and 66 degrees n, but they have been identified in tundra regions as far north as 70 degrees n. all mosquito-borne agents have been bunyaviruses, comprising principally the snowshoe hare subtype of california encephalitis (ce) virus, but also northway virus. mosquito vectors comprise several aedes species and culiseta inornata, all of which h ... | 1975 | 1602 |
| the first isolations of eastern encephalitis, group c, and guama group arboviruses from the peruvian amazon region of western south america. | two strains of eastern encephalitis (ee) virus were isolated in the amazon region of peru near pucallpa, loreto department, using sentinel hamsters. ee virus antibodies were found in healthy horses at both pucallpa and iquitos in the same department. fourteen group c and four guama group arboviruses were recovered from sentenel hamsters and mosquitoes near iquitos. the group c agents were caraparu-ossa, marituba, and oriboca-itaqui viruses, and the guama group agents were bimiti virus. besides p ... | 1975 | 238693 |
| [rna polymerase activity associated with a bunya virus (lumbo)]. | rna dependent rna polymerase has been demonstrated in purified lumbo virus (bunyavirus) which contains a single stranded segmented rna. divalent cations (mn++ and mg++) are required for optimal in vitro activity. reaction products can be specifically annealed with the viral genome. | 1975 | 808281 |
| [ultrastructure, development and nucleic acid of the s virus from the decapod crustacea macropipus depurator l]. | the "s" crabe virus recently isolated from macropipus depurator (decapoda) has been purified and its nucleic acid has been recognized as a dna. the visualization of the structure and of several phases of the cycle of the virus permits to situate this virus near the bunyavirus and paramyxovirus groups. | 1975 | 808338 |
| [circular ribonucleoproteins in the virus lumbo (bunyavirus)]. | 1975 | 808354 | |
| the structure of togaviruses and bunyaviruses. | this article reviews the properties of arthropod-borne and nonarbo togaviruses and members of the bunyaviridae family. averaged information is given on the morphology and substructure of the virion, its physical properties, its chemical composition and the function of its constituents. special attention is given to distinctive traits which might be useful for virus classification. | 1975 | 1107688 |
| the structure of uukuniemi virus, a proposed member of the bunyaviruses. | uukuniemi virus, a proposed member of the new large bunyavirus group of arboviruses, has an interesting structure which differs in many respects from that of other enveloped rna viruses. the surface structure reveals a clustering of the two glycoproteins into distinct capsomere-like subunits, arranged in a t = 12 icosahedral lattice. the genome is found in at least three pieces that appear to represent unique virus-specific rnas. the ribonucleoproteins corresponding to the rna species are circul ... | 1975 | 1107689 |
| the basis of arbovirus classification. | the biologically defined set of arboviruses contains well over 300 separate viruses which have been subdivided into some 40 serological groups on the basis of antigenic cross-reactivity. more than three quarters of all arboviruses can now bw placed into one of the following five major taxonomic genera based upon the fundamental properties of the virion: alphavirus, flavivirus, orbivirus, rhadovirus, bunyavirus. there are 20 alphaviruses, representing serological group a, and 57 flaviviruses in s ... | 1975 | 1207193 |
| bunyaviruses and bunyaviridae. | a new family is described, the bunyaviridae, which contains a single genus, bunyavirus. the main characteristics of the family are as follows: single-stranded rna, total molecular weight about 7 x 10(6) daltons, probably in three segments. virions spherical, enveloped particles 90-100 nm in diameter. envelope contains at least one virus-specified glycopeptide. develop in the cytoplasm, mature by budding into smooth-surfaced vesicles in the golgi region or nearby. internal ribonucleoprotein compo ... | 1975 | 1235823 |
| [demonstration of circular ribonucleic acid in the lumbo virus (bunyavirus)]. | electron microscopy study of rna extracted from lumbovirus shows the existence of circular molecules, which are not susceptible to thermal or chemical denaturation. these facts suggest that the rna is present inside the virion in a covalently closed circular form. | 1976 | 820471 |
| california encephalitis virus proliferation in yukon mosquitoes incubated at low temperatures. | replication of a subarctic bunyavirus, california encephalitis (snowshoe hare subtype), was detected in salivary glands and thoraces of wild-caught aedes communis mosquitoes from the yokon territory, after intrathoracic inoculation with 0.1 to 100 mouse ld50 virus, and incubation for 7 to 21 days throughout their viable temperature range of 0 to 23 degrees c. immunoperoxidase staining confirmed that viral replication occurred in the cytoplasm of acinar cells of salivary glands, both by ligh micr ... | 1976 | 963625 |
| structural proteins of la crosse virus. | preparations of la crosse virus, a member of the california encephalitis group of bunyaviruses, were found to possess three major virion proteins. two of the proteins were glycosylated (g1 and g2) and were located on the surface of the virus particles. these two glycoproteins were present in equimolar amounts and possessed apparent molecular weights of 120 x 10(3) and 34 x 10(3). virion nucleocapsids, isolated by a nonionic detergent and salt treatment, contained another major protein, n (molecu ... | 1976 | 972436 |
| recombination and complementation between temperature-sensitive mutants of a bunyavirus, snowshoe hare virus. | complementation and recombination have been observed with temperature-sensitive mutants of bunyavirus snowshoe hare virus. | 1976 | 978796 |
| congenital abnormalities in newborn lambs after infection of pregnant sheep with akabane virus. | akabane virus (a bunyavirus) has been associated with epizootics of congenital deformities in cattle, sheep, and goats. experimental studies using mouse-adapted virus inoculated intravenously into pregnant sheep gave an inapparent infection. neutralizing antibodies were detected on day 5, and peaks in the titer were seen at days 10 and 48. ewes infected at day 30 to 36 of pregnancy produced five (31% incidence) deformed lambs. sera from four of these possessed neutralizing antibodies to akabane ... | 1977 | 832900 |
| the virus particle nucleic acids and proteins of four bunyaviruses. | the structural polypeptides of five bunyaviruses, snowshoe hare, lumbo and la crosse viruses (members of the california encephalitis subgroup of bunyaviruses), bunyamwera and main drain viruses (members of the bunyamwera subgroup of bunyaviruses), have been compared by polyacrylamide-sds gel electrophoresis. each virus was found to possess three major structural polypeptides, two glycoproteins (g1 and g2), and one nucleocapsid protein (n). although the sizes of the g1 polypeptides (mol. wt. appr ... | 1977 | 839199 |
| electron microscopy of the segmented rna genome ofla crosse virus: absence of circular molecules. | the three species of single-stranded rna present in la crosse virus were examined in the electron microscope. because large amounts of contaminating cellular dna are copurified with the virus despite extensive attempts to purify the virus, it was necessary to use procedures that eliminated the bulk of this dna before the viral rna was analyzed. when this was done, the modal lengths of la crosse virus rna were 0.4, 2.0, and 3.1 mum. these lengths correspond well to their known molecular weights o ... | 1977 | 853565 |
| immune electron microscopy of arboviruses. | representative members of four arbovirus taxons were studied by immune electron microscopy (iem) in an attempt to determine whether the technique can be applied to the specific identification of arboviruses. alphavirus, flavivirus, bunyavirus, and rhabdovirus antigens were prepared by propagation in tissue culture. the viruses were used to titrate homologous antibody and test heterologous antibody in the iem test. specific clumping together with visualization of antibody attachment was observed ... | 1977 | 889020 |
| three unique viral rna species of snowshoe hare and la crosse bunyaviruses. | two-dimensional gel electrophoreses of rnase t1-derived oligonucleotides of the three individual rna segments of the bunyavirus snowshow hare virus indicate that its three rna segments possess distinct nucleotide sequences. the fingerprints of the rna species of snowshoe hare virus differ from those of the antigenically closely related la crosse virus. three viral rna species have been identified in preparations of melao and trivittatus as well as snowshoe hare, lumbo, and la crosse bunyaviruses ... | 1977 | 559104 |
| formation of recombinants between snowshoe hare and la crosse bunyaviruses. | wild-type recombinants were obtained at high frequency from coinfections of bhk cells involving temperature-sensitive, conditional-lethal mutants of snowshoe hare (ssh) and la crosse (lac) bunyaviruses. analyses of two of the recombinants indicated that they have the genome compositions ssh/lac/ssh and ssh/lac/lac for their respective l, m, and s virion rna species. this evidence, together with that for the genetic stability of the recombinants, indicates that they were derived by segment reasso ... | 1977 | 592468 |
| bunyavirus isolations from mosquitoes in the western canadian arctic. | strains of california encephalitis virus (snowshoe hare subtype) were isolated from 8 of 475 pools comprising 23 747 unengorged female mosquitoes of five species collected at three of six locations throughout the mackenzie valley of the northwest territories, canada, from latitudes 60 to 69 degrees n between 10 and 24 july 1976. minimum field infection rates included 1:2734 for aedes communis, 1:256 to 1:3662 for a. hexodontus and 1:911 to 1:1611 for a. punctor. northway virus was also isolated ... | 1977 | 18541 |
| khasan virus, a new ungrouped bunyavirus isolated from haemaphysalis longicornis ticks in the primorie region. | strain leiv-776p, possessing no haemagglutinating activity was isolated in 1971 in the primorie region (u.s.s.r.) from haemaphysalis longicornis neumann 1091 tick. complement fixation (cf) tests revealed no antigenic relationship with 24 antigenic groups of arboviruses or 21 ungrouped viruses isolated from ticks. the virus isolate contains rna and is relatively sensitive to ether and sodium deoxycholate; it is pathogenic for suckling mice and two-week-old white mice by the intracerebral route, a ... | 1978 | 27977 |
| experimental infection of putorius eversmanni polecats and martes foina martens with tahyna virus. | three putorius eversmanni pole-cats and two martes foina martens aged about 9 months were subcutaneously infected with about 260 suckling mouse ld50 of the extraneurally passaged "236" strain of tahyna virus (california group, genus bunyavirus). viraemia with maximal titres of 1.32 (pole-cats) and 1.28 (martens) dex intraperitoneal (i.p.) mouse ld50/0.02 ml was demonstrated from 48 to 96 hr after inoculation (p.i.). by the plaque-reduction neutralization test, seroconversion was demonstrated 15 ... | 1978 | 35949 |
| razdan virus, a new ungrouped bunyavirus isolated from dermacentor marginatus ticks in armenia. | a virus, designated razdan, was isolated from dermacentor marginatus ticks in the armenian s.s.r. in 1973. the complement fixation tests revealed no antigenic relationships to 74 tick-borne arboviruses. the size of the virus is about 100 nm; it agglutinates goose erythrocytes at ph 5.5--7.0, is pathogenic for newborn, 14-day-old and adult white mice and multiplies in primary and continuous cell cultures. morphological properties of the virus permit its classification as a member of the family bu ... | 1978 | 35950 |
| discontinuous counter-immunoelectrophoresis in the study of viruses. | a method for the use of the discontinuous counter-immunoelectropnoresis (dcie) technique in the study of viruses of the major taxonomic groups is reported. the method was successfully employed in detection of antigen of viruses in the bunyavirus, bunyavirus-like, flavivirus, orbivirus, and rhabdovirus groups, and/or of antibody to these agents. although less sensitive than the complement fixation test, the dcie test was found to be rapid and in some cases more specific in differentiating related ... | 1978 | 97243 |
| induction of interferon by group c arboviruses. brief report. | 1978 | 104697 | |
| investigations on the presence of antibodies to alphaviruses, flaviviruses, bunyavirus and kemerovo virus in humans and some domestic animals. | the presence of antibodies to some alphaviruses, flaviviruses, bunyaviruses and orbiviruses was studied in the sera from humans and domestic animals of biotopes "c" and "t" in the south-east of romania. specific antibodies were found in humans to 3 alphaviruses (eastern equine encephalitis, western equine encephalitis and sindbis), in a very low proportion, and to 3 flaviviruses (tickborne encephalitis, west nile and ntaya), at a much higher percentage. very low percentages of antibodies to buny ... | 1978 | 209613 |
| antibody response to arboviruses. absence of increased response in amyotrophic lateral sclerosis and multiple sclerosis. | complement fixation and hemagglutination imhibition tests were conducted on the serums of patients with amyotrophic lateral sclerosis and multiple sclerosis using a variety of arboviral antigens. seventy-eight complement fixation and 15 hemagglutination-inhibition viral antigens were used representing togaviruses, orbiviruses, rhadoviruses, bunyaviruses, arenaviruses, and several ungrouped agents. the serological results did not indicate any relationship between these viruses and either amyotrop ... | 1978 | 666595 |
| transmission of northway and st. louis encephalitis viruses by arctic mosquitoes. | transmission of a canadian arctic isolate of northway virus has been demonstrated after incubation of arctic aedes communis mosquitoes at 13 degrees c for 27 days after intrathoracic injection of 300 plaque forming units of virus. replication has also been demonstrated after intrathoracic injection of domestic a. aegypti mosquitoes of this virus. virions of northway virus, 84--92 nm diameter were morphologically typical of a bunyavirus after propagation in salivary glands of a. communis or in ti ... | 1978 | 687117 |
| small viral rna segment of bunyaviruses codes for viral nucleocapsid protein. | tryptic peptide analyses have been undertaken on the nucleocapsid (n) protein of snowshoe hare (ssh) and la crosse (lac) bunyaviruses. similar analyses have been performed on the n proteins of two recombinant viruses which have the large/medium/small rna genome configurations: ssh/lac/lac and ssh/lac/ssh. the results provide conclusive evidence that the s rna of bunyaviruses codes for the the viral n protein. | 1978 | 702656 |
| serological comparison of aino and samford viruses in simbu group of bunyaviruses. | 1978 | 739909 | |
| genetic capabilities of bunyaviruses. | 1978 | 752701 | |
| seriologic and vector comparisons of rift valley virus with other bunyaviruses. | 1978 | 752702 | |
| biochemical studies on the phlebotomus fever group viruses (bunyaviridae family). | analyses of the virion polypeptides and genomes of several phlebotomus fever group viruses, karimabad, punta toro, chagres, and the sandfly fever sicilian serotype viruses, have established that they are biochemically similar to the accepted members of the bunyaviridae family. like snowshoe hare virus (a member of the california serogroup of the bunyavirus genus of the bunyaviridae family), karimabad, punta toro, chagres, and the sandfly fever sicilian serotype viruses all have three viral rna s ... | 1979 | 225523 |
| tonate and guama-group viruses isolated from mosquitoes in both a savannah and coastal area in surinam. | isolation of arboviruses was attempted from october 1972 till the end of 1973 from mosquitoes caught in both a savannah forest near powaka and a coastal area near "fernandesweg" in surinam. two types of arbovirus were isolated: eleven strains of tonate and ten strains of guama-group. in the savannah area ten strains of tonate and five of guama-group were isolated, whereas in the coastal area the numbers were one and five, respectively. all strains in the savannah area were isolated from pools of ... | 1979 | 41349 |
| bunyavirus development in arctic and aedes aegypti mosquitoes as revealed by glucose oxidase staining and immunofluorescence. | northway virus replication has been detected in salivary glands of wild-caught culiseta inornata and aedes communis mosquitoes from the western canadian arctic after incubation at 4 degrees c for 9 to 11 months, and after incubation at 13 degrees c for 3 to 4 months after they received virus by oral ingestion or intrathoracic injection. aedes hexodontus supported northway virus replication after incubation at 13 degrees c for one month after intrathoracic injection. aedes aegypti supported north ... | 1979 | 44464 |
| genetic potential of bunyaviruses. | 1979 | 387343 | |
| study of arboviruses in romania (1958--1978). results obtained in the "stefan s. nicolau" institute of virology. | the results obtained in the institute of virology by the study of arboviruses in romania are reviewed. isolation of tick-borne encephalitis virus and detection of specific serum antibodies in humans, animals and migratory birds demonstrate its presence on the territory of romania. serum antibodies could also be detected to other three flaviviruses (west nile, ntaya, dengue types 1, 2, 3), though none of them could be isolated in our country. however, isolation of kemerovo and tahyna viruses and ... | 1979 | 392912 |
| a comparison of la crosse virus isolated obtained from different ecological niches and an analysis of the structural components of california encephalitis serogroup viruses and other bunyaviruses. | analyses of the oligonucleotide fingerprints of the three genome ribonucleic acid (rna) species of 11 isolates of la crosse (lac) virus, obtained from various ecological niches in the northern united states and compared to those of prototype lac virus, showed that in each place from which these isolates were obtained lac variants and varieties were present with related, but distinguishable, nucleotide sequences for their large, medium, or small rna species. the rna genomes of prototypes trivitta ... | 1979 | 453439 |
| heterologous interference in aedes albopictus cells infected with alphaviruses. | maximum amounts of 42s and 26s single-stranded viral rna and viral structural proteins were synthesized in aedes albopictus cells at 24 h after sindbis virus infection. thereafter, viral rna and protein syntheses were inhibited. by 3 days postinfection, only small quantities of 42s rna and no detectable 26s rna or structural proteins were synthesized in infected cells. superinfection of a. albopictus cells 3 days after sindbis virus infection with sindbis, semliki forest, una, or chikungunya alp ... | 1979 | 480461 |
| m viral rna segment of bunyaviruses codes for two glycoproteins, g1 and g2. | tryptic peptide digests of the two viral glycoproteins (g1 and g2) of snowshow hare (ssh) virus, la crosse, la crosse (lac) virus, and an ssh/lac recombinant virus which has a large (l)/medium (m)/small (s) rna segment genome composition of ssh/lac/ssh were analyzed by ion-exchange column chromatography. the analyses prove that the m rna species of bunyaviruses codes for the two viral glycoproteins. | 1979 | 480466 |
| in vivo transcription and protein synthesis capabilities of bunyaviruses: wild-type snowshoe hare virus and its temperature-sensitive group i, group ii, and group i/ii mutants. | the in vivo primary and secondary transcription capabilities of wild-type snowshoe hare (ssh) virus and certain of its temperature-sensitive (ts) mutants have been analyzed. the results obtained agree with in vitro studies (bouloy et al., c.r. acad. sci. paris 280:213-215, 1975; m. bouloy and c. hannoun, virology 69:258-264, 1976; m. ranki and r. pettersson, j. virol. 16:1420-1425, 1975) which have shown that bunyaviruses are negative-stranded rna viruses with a virion rna-directed rna polymeras ... | 1979 | 480477 |
| genome complexities of the three mrna species of snowshoe hare bunyavirus and in vitro translation of s mrna to viral n polypeptide. | the genome complexities of the principal intracellular viral complementary rna species of the snowshoe hare bunyavirus have been analyzed by duplex analyses involving hybridization of complementary rna to individual 32p-labeled viral rna species (large, l; medium, m; and small, s), recovery of nuclease-resistant duplexes, and determination of the oligonucleotide fingerprints of the protected 32p-labeled viral sequences. the result for the m rna (which codes for the glycoproteins g1 and g2; j. r. ... | 1979 | 513192 |
| recombination between snowhoe hare and la crosse bunyaviruses. | we have previously reported heterologous genetic recombination resulting from crosses involving temperature-sensitive (ts) mutants of la crosse (lac) group ii and snowshoe hare (ssh) group i ts mutants (j. gentsch, l. r. wynne, j. p. clewley, r. e. shope, and d. h. l. bishop, j. virol. 24:893-902, 1977). from those crosses two reassortant viruses having the large/medium/small viral rna segment genotypes of ssh/lac/ssh and ssh/lac/lac were obtained. in this study it has been found that the recipr ... | 1979 | 513193 |
| arboviruses of coastal south-eastern australia. | during investigations of epidemic polyarthritis at nelson bay, new south wales, 12 strains of ross river virus, the causative agent, were recovered from pools of mosquitoes. in addition, the mosquito pools yielded 9 strains of the flavivirus edge hill, 4 strains of a bunyavirus, gan gan, 1 strain of an orbivirus tilligerry, and 1 strain of an ungrouped probable arbovirus, yacaaba. the latter 3 viruses were found to be antigenically distinct from previously recorded arboviruses. a case of epidemi ... | 1980 | 6108758 |
| bunyaviridae. | the family bunyaviridae comprises over 200 viruses (serotypes, subtypes, and varieties) that infect vertebrates and/or invertebrates. four genera of viruses have been defined (bunyavirus, nairovirus, phlebovirus, and uukuvirus). the main characteristics of the member viruses are: (i) the virus particles are for the most part uniformly spherical, 80-110 nm in diameter, and possess a unit membrane envelope from which protrude polypeptide spikes 5-10nm long; (ii) the viruses have three helical nucl ... | 1980 | 6165702 |
| carbon dioxide sensitivity of mosquitoes infected with california encephalitis virus. | four species of mosquitoes became sensitive to carbon dioxide approximately 3 to 4 days after they received intrathoracic injectins of california encephalitis virus. aedes melanimon and aedes dorsalis infected orally with california encephalitis virus also became carbon dioxide-sensitive, but mosquitoes infected transovarially did not. sensitivity to carbon dioxide was inhibited by antiserum to california encephalitis virus. to our knowledge this is the first report of carbon dioxide sensitivity ... | 1980 | 6773145 |
| tataguine virus infection in mice and hamsters. | suckling hamsters, weanling and suckling mice, inoculated intracerebrally with tataguine virus consistently developed fatal infections with average survival time of 3-8 days. deaths were observed in a few suckling hamsters infected by the intraperitoneal route. infectivity assays and fluorescent antibody staining of organs of infected hamsters showed that the brain was the only organ in which viral replication occurred. histopathological lesions found in the brain of such mice and hamsters infec ... | 1980 | 6776388 |
| characterization of the viral ribonucleic acids and structural polypeptides of anopheles a, bunyamwera, group c, california, capim, guama, patois, and simbu bunyaviruses. | analyses of the viral ribonucleic acids and structural polypeptides of 17-22 of the 119 accepted or proposed members of the bunyavirus genus of arboviruses (family bunyaviridae), have shown that from the standpoint of their structural components these viruses are highly comparable to each other. the average molecular weights for the three viral rna species (l, large, m, medium, s, small) of 17 bunyaviruses were 2.93 x 10(6) (l, range 2.7-3.1 x 10(6)), 2.0 x 10(6) (m, range 1.8-2.3 x 10(6)), and ... | 1980 | 6778231 |
| recombination and gene coding assignments of bunyaviruses and arenaviruses. | 1980 | 7013623 | |
| evidence from recombinant bunyavirus studies that the m rna gene products elicit neutralizing antibodies. | 1980 | 7368566 | |
| the effect of glycosylation inhibitors on the maturation and intracellular polypeptide synthesis induced by snowshoe hare bunyavirus. | 1980 | 7368577 | |
| analyses of the 3'-terminal sequences of snowshoe hare and la crosse bunyaviruses. | 1980 | 7423860 | |
| monoclonal anti-fc receptor igg blocks antibody enhancement of viral replication in macrophages. | flaviviruses, when complexed with antibody at subneutralizing concentrations, show enhanced replication in human and simian peripheral blood leukocytes (ref. 1, and j.s.m.p. and j.s.p., unpublished observations) and in p388 d1 and other macrophage cell lines. a comparable phenomenon has been demonstrated with alphaviruses and bunyaviruses in p388 d1 cells, (j.s.m.p. and j.s.p., unpublished observations) but cells lacking macrophage characteristics fail to show antibody-dependent enhancement (ade ... | 1981 | 7453820 |
| analyses of patois group bunyaviruses: evidence for naturally occurring recombinant bunyaviruses and existence of immune precipitable and nonprecipitable nonvirion proteins induced in burnyavirus-infected cells. | 1981 | 7222477 | |
| attenuation of virulence of a bunyavirus involving an l rna defect and isolation of lac/ssh/lac and lac/ssh/ssh reassortants. | 1981 | 7233829 | |
| formation of reassortant bunyaviruses in dually infected mosquitoes. | 1981 | 7245612 | |
| radioimmune assays and molecular studies that place anopheles b and turlock serogroup viruses in the bunyavirus genus (bunyaviridae). | molecular analyses indicate that turlock virus (tur, turlock serogroup) and boraceia virus (bor, anopheles b serogroup) have virion rna species and polypeptides comparable in size to those of members of the bunyavirus genus and unlike those of members of the newly defined phlebovirus, nairovirus, or uukuvirus genera (bunyaviridae). the 11 terminal 3' end nucleotides of the three virion rna species of both bor and tur viruses (houcaucacaug...) are identical in sequence to the 3' end sequences of ... | 1981 | 7258499 |
| structural polypeptides of hazara virus. | four structural polypeptides of hazara virus, an agent closely related to the crimean-congo haemorrhagic fever (c-chf) viruses, were resolved by sds-polyacrylamide gel electrophoresis. three glycoproteins were identified (mol. wt. 84,000, 45,000 and 30,000) and were found to be associated with the virion envelope. a fourth polypeptide (mol. wt. 52,000) was non-glycosylated and associated with the nucleocapsid. the structural proteins of hazara virus differ markedly from those reported for other ... | 1981 | 7276913 |
| role of the middle-sized bunyavirus rna segment in mouse virulence. | 1981 | 7281516 | |
| comparisons of belmont virus, a possible bunyavirus unique to australia, with bunyamwera virus. | belmont virus is an arbovirus isolated from mosquitoes and has a preference for marsupial hosts. the diameter of virions by negative staining (122 nm before fixation and 91 nm after fixation) was greater than that of bunyamwera virus (94 nm and 79 nm respectively). however, the particles of both viruses appeared morphologically identical and sedimented at the same rate in sucrose density gradients. belmont virus had a tripartite segmented rna genome (28s, 24s and 11s) similar to bunyamwera virus ... | 1981 | 7288402 |
| proteins and glycoproteins specified by bunyamwera virus and by belmont virus, a possible bunyavirus, in mammalian cells. | purified preparations of belmont virus were shown to be very similar morphologically and biochemically to those of bunyamwera virus. comparisons of the synthesis of virus-specified proteins in vero and bhk-21 cells confirm the close taxonomic relationship. total protein synthesis was inhibited 95% by 23 h post-infection with belmont virus; a similar reduction occurred earlier in bunyamwera virus-infected cells. this inhibition was multiplicity dependent, and synthesis of the host protein compone ... | 1981 | 7288403 |
| structural characteristics of nairoviruses (genus nairovirus, bunyaviridae). | viruses from six antigenic groups of arthropod-borne viruses [crimean--congo haemorrhagic fever (cchf), nairobi sheep disease (nsd), qalyub (qyb), sakhalin (sak), dera ghazi khan (dgk) and hughes (hug) serogroups], some previously categorized as bunyavirus-like viruses and others previously ungrouped, have recently been assembled by serological analyses into a new genus of viruses (nairovirus genus) in the bunyaviridae. molecular studies of the virion rna and viral polypeptides have been underta ... | 1981 | 7299367 |
| orbiviruses and bunyaviruses from a seabird colony in scotland. | viruses isolated from ticks (ixodes uriae) and a kittiwake (rissa tridactyla) from a seabird colony at st. abb's head, scotland, were shown by complement fixation tests (cft) to be antigenically related to the uukuniemi and kemerovo serogroups. electron microscopic examination of cell cultures infected with the kemerovo group viruses revealed particles characteristic of orbiviruses, 72 +/- 3 nm in diam., with an inner core 37 +/- 3 nm in diam., in association with intracytoplasmic, densely stain ... | 1981 | 7320703 |
| antigenic relationships among simbu serogroup (bunyaviridae) viruses. | antigenic relationships among 24 bunyaviruses of the simbu serogroup were determined by complement-fixation (cf), serum dilution-plaque reduction neutralization (n) and, where possible, hemagglutination-inhibition (hi) tests. by cf, three distinct complexes of closely related viruses were identified within the serogroup. nola and thimiri viruses, which showed little relationship with other members of the serogroup, may represent two additional complexes. n tests in vero cells showed that individ ... | 1981 | 7325288 |
| molecular basis of bunyavirus transmission by mosquitoes: role of the middle-sized rna segment. | in an examination of the molecular basis of oral transmission of bunyaviruses by mosquitoes., la crosse (lac), snowshoe hare (ssh), and lac-ssh reassortant viruses were compared in their ability to be transmitted to laboratory mice by the natural mosquito vector of lac virus, aedes triseriatus. both lac virus and the reassortment viruses containing the middle-sized (m) segment from the lac parent were efficiently transmitted. in contrast, ssh virus and reassortment viruses containing the m rna f ... | 1981 | 6781068 |
| [bunyaviruses]. | 1981 | 6798775 | |
| [transovarial transmission of arboviruses by mosquitoes (author's transl)]. | an important aspect of the epidemiology of arboviruses is the manner in which the viruses are maintained during winter, dry season, or other adverse environmental periods when their arthropod hosts are inactive. one possibility is that the viruses survive in arthropods. in the case of mosquito-borne viruses, it is probable that such viruses could be maintained in this manner only if they were transmitted from one insect generation to the next by transovarial transmission. such transmission was r ... | 1981 | 6116146 |
| guamá-group virus activity in surinam. | from october 1972 through december 1973 entomological surveys were carried out in both a savannah and coastal area in surinam. attempts were made to isolate arboviruses from the captured mosquitoes. the results have been published elsewhere (panday and digoutte, 1979). the present paper deals with the further typing of the obtained guamá-group viruses. preliminary typing was done by means of complement-fixation test. by performing the neutralization test in mice, the unknown guamá-group viruses ... | 1981 | 6116303 |
| use of hyperimmune mouse ascitic fluids for arbovirus differentiation by indirect immunofluorescence and conventional serology. | the cross-reactivity of 22 arbovirus species (alphaviruses; tick- and mosquito-borne flaviviruses; and selected bunyaviruses) was tested with monovalent immune mouse ascitic fluids by indirect immunofluorescence (iif) in comparison with classical serological reactions (virus neutralization -- vn; haemagglutination inhibition -- hi; and complement fixation -- cf -- reactions). known relationships within the virus groups studied were confirmed. as to the differentiation limits, the vn test was fol ... | 1981 | 6118048 |
| analyses of patois group bunyaviruses: evidence for naturally occurring recombinant bunyaviruses and existence of immune precipitable and nonprecipitable nonvirion proteins induced in bunyavirus-infected cells. | shark river (sr) and pahayokee (pah) bunyaviruses (patois serogroup, bunyavirus genus, family bunyaviridae) have almost identical l and s rna oligonucleotide fingerprints, but m rna fingerprints that are different, suggesting that the two viruses may represent naturally occurring reassortant viruses. these observations are in agreement with serological studies (b. n. fields, b. e. henderson, p. h. coleman, and t. h. work, 1969, amer. j. epidemiol., 89, 222-226) which have distinguished these two ... | 1981 | 18635048 |
| relationship between glycoproteins of the viral envelope of bunyaviruses and antibody-dependent plaque enhancement. | hamster antisera against three parental bunyaviruses, batai, bunyamwera and maguari viruses, and six recombinant viruses which carried the nucleocapsid protein of one parent and the glycoproteins of the other, have been tested for their interaction with each of the nine viruses under study by two assays, plaque reduction neutralization and antibody-dependent plaque enhancement. neutralization was clearly related to the specificity of the parental glycoproteins rather than the nucleoprotein, but ... | 1982 | 6890986 |
| molecular basis of bunyavirus per os infection of mosquitoes: role of the middle-sized rna segment. | the molecular basis of bunyavirus per os infection of mosquitoes was determined; la crosse (lac), snowshoe hare (ssh), and lac-ssh reassortment viruses were compared for their ability to infect aedes triseriatus, the natural vector of the lac virus. the viruses were comparable in their ability to infect midgut cells; 115 of 117 (98%) mosquitoes ingesting viruses containing the lac middle-sized rna segment and 92/100 (92%) of mosquitoes ingesting viruses containing the ssh middle-sized rna segmen ... | 1982 | 6951175 |
| sensitivity to carbon dioxide in mosquitoes infected with california serogroup arboviruses. | ten species of mosquitoes became sensitive to co2 following intrathoracic (i.t.) inoculation of california encephalitis (ce) virus. these included field-collected aedes melanimon, aedes nigromaculis and culiseta incidens and laboratory-colonized strains of aedes dorsalis, aedes triseriatus, anopheles freeborni, culex peus, culex pipiens pipiens, culex pipiens quinquefasciatus and culex tarsalis. another california serogroup virus, jerry slough (= jamestown canyon) (js), also induced co2 sensitiv ... | 1982 | 6122383 |
| the complete sequence and coding content of snowshoe hare bunyavirus small (s) viral rna species. | the complete sequence of the small (s) viral rna species of snowshoe hare (ssh) bunyavirus has been determined, principally from a dna copy of the rna cloned in the e.coli plasmid pbr322. the viral s rna (negative sense strand) is 982 nucleotides long (3.3 x 10(5) daltons) with complementary 5' and 3' end sequences. it has a base composition of 30.5%u, 25.8%a, 24.9%c and 18.7%g. in the viral complementary (plus sense) strand there are two overlapping open reading frames initiated by methionine c ... | 1982 | 7050911 |
| identification of virus-coded nonstructural polypeptides in bunyavirus-infected cells. | analyses of bunyavirus-infected cell extracts identified at least two virus-induced nonstructural polypeptides. with snowshoe hare (ssh), la crosse (lac), and six ssh-lac reassortant viruses, it was shown that one of these nonstructural polypeptides (nss, approximate molecular weight, 7.4 x 10(3)) is coded by the ssh small (s)-size viral rna species. this nonstructural polypeptide was not detected (at least in the same relative abundancies) in lac virus-infected cells or in cells infected with r ... | 1982 | 7077749 |
| nucleotide sequence analyses and predicted coding of bunyavirus genome rna species. | we performed 3' rna sequence analyses of [(32)p]pcp-end-labeled la crosse (lac) virus, alternate lac virus isolate l74, and snowshoe hare bunyavirus large (l), medium (m), and small (s) negative-stranded viral rna species to determine the coding capabilities of these species. these analyses were confirmed by dideoxy primer extension studies in which we used a synthetic oligodeoxynucleotide primer complementary to the conserved 3'-terminal decanucleotide of the three viral rna species (clerx-van ... | 1982 | 7086954 |
| electron microscopic study of bluegill virus. | bluegill virus (bgv) grown in bf-2 cells was studied by negative staining and ultrathin sections of infected cells. although bgv resembles bunyaviruses in gross physical appearance, it differs from this group in several important aspects. thus, bgv cannot be classified as a member of the bunyaviridae family and could be a representative of a novel family of enveloped rna viruses. | 1982 | 7093819 |
| mutant identifying a third recombination group in a bunyavirus. | only two recombination groups have been reported in genetic analyses of ts mutants of 10 different bunyaviruses from the bunyamwera and california encephalitis serogroups, although three groups are expected from the tripartite structure of the genome of all members of the family bunyaviridae. we describe now a ts mutant of maguari virus, magts23(iii), which recombined in both vertebrate (bhk-21) and invertebrate (aedes albopictus) cells with mutants representing recombination groups i and ii of ... | 1982 | 7097862 |
| characterization of monoclonal antibodies against the g1 and n proteins of lacrosse and tahyna, two california serogroup bunyaviruses. | 1982 | 7101727 | |
| the 3' terminal rna sequences of bunyaviruses and nairoviruses (bunyaviridae): evidence of end sequence generic differences within the virus family. | the 3' terminal nucleotide sequences of the three virus rna species of viruses representing eight serogroups of bunyaviruses (genus bunyavirus, bunyaviridae) and six serogroups of nairoviruses (genus nairovirus, bunyaviridae) have been characterized. members of the bunyavirus genus have conserved 3' end sequences (generally, 3' ucaucacauga...) that differ from the conserved 3' end sequences of members of the nairovirus genus (generally, 3' agaguuucu...). | 1982 | 7119754 |
| panhandles and hairpin structures at the termini of germiston virus rnas (bunyavirus). | 1982 | 7135833 | |
| characterization of the oligosaccharides of inkoo virus envelope glycoproteins. | inkoo virus (a bunyavirus) was grown in bhk-21 cells and labelled with [35s]methionine or [3h]mannose. [35s]methionine labelled the two envelope glycoproteins g1 (mr = 125000) and g2 (mr = 35000), as well as the nucleocapsid protein n (mr = 25000). only g1 and g2 were labelled with the sugar precursor. the [3h]mannose-labelled virus was solubilized with detergent and digested with pronase. the structure of the labelled glycopeptides originating from the mixture of g1 and g2 was studied by degrad ... | 1982 | 7153764 |
| seven infection-specific polypeptides in bhk cells infected with bunyamwera virus. | virus-specific polypeptide synthesis was examined in bhk cells and vero cells infected with bunyamwera virus. in bhk cells, in addition to the four previously reported virus-coded proteins (l, g1, g2, and n), three other infection-specific proteins were detected. these proteins, of nominal molecular weight 50,000 (p50), 16,000 (p16), and 13,000 (p13), were not labeled in mock-infected cells, were first synthesized between 4 and 8 h after infection, and were relatively prominent among the limited ... | 1982 | 6183441 |
| [mechanisms of in vivo suppressive effect of togaviridae and bunyaviridae on the activity of effectors of graft vs host reaction]. | experiments on mice demonstrated the ability of 3 flaviviruses and 1 bunyavirus to suppress the activity of the effectors of the graft-versus-host (gvh) reaction. the conditions of the suppression of the primary immunological recognition were shown to differ in infections caused by different viruses. in experimental flavivirus infections caused by langat, dengue 2 or yellow fever (strain 17d) viruses t-suppressor cells were activated, and their activity was realized only in respect to syngeneic ... | 1982 | 6283771 |
| orbi- and bunyaviruses from a puffin colony in the outer hebrides. | 1982 | 6299238 | |
| viruses recovered from mosquitoes and wildlife serum collected in the murray valley of south-eastern australia, february 1974, during an epidemic of encephalitis. | pools of mosquitoes collected in the murray valley in february, 1974, during an encephalitis epidemic yielded 239 isolates of 11 distinct viruses. these included 39 isolates of mve virus, an incriminated causative agent of encephalitis in man, and 111 isolates of kunjin virus, a probable causative agent. an additional isolate of mve virus was recovered from the serum of a white-faced heron, ardea novaehollandiae. the other 9 viruses comprised the alpha-viruses ross river and sindbis, the flavivi ... | 1982 | 6299258 |
| [characteristics of bunyavirus- and togavirus-induced nonspecific suppressors inhibiting delayed hypersensitivity]. | some properties and mechanisms of action of nonspecific suppressor cells, inhibiting delayed hypersensitivity to sheep red blood cells and activated in vivo in experimental tick-borne encephalitis and tahyna virus infections in mice, have been studied. these nonspecific suppressor cells have been identified as t-lymphocytes in experiments with the use of antisera to t- and b-lymphocytes. the function of the suppressor cells can be realized without their proliferation and is mediated by a soluble ... | 1982 | 6213121 |
| bunyavirus nucleoprotein, n, and a non-structural protein, nss, are coded by overlapping reading frames in the s rna. | it has been shown previously, by sequence analysis of the s rna segment of snowshoe hare (ssh) bunyavirus, that two overlapping open reading frames in the viral complementary sequence code for proteins with molecular weights of 26.8 x 10(3) and 10.5 x 10(3) respectively. in addition to the viral nucleocapsid (n) protein, which is coded by the s rna, analyses of parental and reassortant bunyavirus-infected cell extracts have shown that the viral s rna and m rna species each code for non-structura ... | 1983 | 6223987 |
| [human disease caused by nepuyo virus, a central american bunyavirus transmitted by mosquitoes]. | 1983 | 6226296 | |
| taxonomy, classification, and geographic distribution of california serogroup bunyaviruses. | 1983 | 6346334 | |
| the effect of proteolytic cleavage of la crosse virus g1 glycoprotein on antibody neutralization. | the envelope of the bunyavirus la crosse contains two glycoproteins, g1 (120 000 mol. wt.) and g2 (38 000 mol. wt.). when incubated with trypsin or plasmin, the g1 glycoprotein of virus grown in cell culture was cleaved, leaving two different sized polypeptides in the envelope (67 000 and 95 000 mol. wt.). chymotrypsin cleaved g1 leaving polypeptides of 70 000 and 100 000 mol. wt. g2, however, was not altered by these enzymes. when used in antibody neutralization studies, these proteolytically m ... | 1983 | 6352863 |
| [conditions for preserving the simbu group of bunyaviruses]. | 1983 | 6670261 | |
| identification of new guama and group c serogroup bunyaviruses and an ungrouped virus from southern brazil. | from 1975 to 1978, 36 viruses were recovered from humans, bats, birds, sentinel mice and hamsters, and from mosquitoes collected in coastal brazil in the state of são paulo. identifications of 22 of these 36 viruses have been reported. six of the remaining 14 isolates were shown to be guama serogroup bunyaviruses. two of these six were strains of a newly recognized virus for which the name cananeia virus is proposed; another is a second newly recognized guama serogroup virus for which the name i ... | 1983 | 6404190 |
| characterization of leanyer virus: resemblance to bunyavirus. | the properties of leanyer virus, isolated in northern australia in 1974, were compared with those of bunyamwera virus. leanyer virus replicated in bhk-21 and vero cells. in sucrose gradients it had a density of 1.17 g/cm3 and sedimented with the same s value as bunyamwera virus. the diameter of negatively stained virions was approximately 110 nm. three species of rna sedimenting at 30s (l), 26s (m) and 14s (s) and four virion proteins (l, g1, g2, n) were detected in preparations of purified viri ... | 1983 | 6411054 |
| the mapputta group of arboviruses: ultrastructural and molecular studies which place the group in the bunyavirus genus of the family bunyaviridae. | we have characterized members of the mapputta group of 'bunyavirus-like' viruses in terms of morphology, structure, ultrastructural development and virus-directed rna and protein synthesis. our primary study has been with maprik virus (mpk) as a representative of the group. the mpk virion is uniformly spherical (congruent to 90 nm diameter) and possesses a membrane envelope. virus maturation is by budding into small vesicles in the perinuclear region. during infection of bhk cells which is cytop ... | 1983 | 6411055 |
| analysis of hantaan virus rna: evidence for a new genus of bunyaviridae. | hantaan virus, the prototype virus of hemorrhagic fever with renal syndrome, was examined for nucleic acid characteristics which would support its previously proposed inclusion in the virus family bunyaviridae. nucleocapsid rna from hantaan virions and a control bunyavirus were examined for ribonuclease a (rnase a) sensitivity. both viruses exhibited a similar accessibility of rna within nucleocapsids to digestion by rnase a. complete digestion of the rna of both viruses was affected with high c ... | 1983 | 6419460 |
| [effect of ribavirin on bunyavirus reproduction in cell culture and in an experiment on white mice]. | 1983 | 6419466 | |
| interference between bunyaviruses in aedes triseriatus mosquitoes. | inhibition of the replication of alternate california serogroup bunyaviruses in aedes triseriatus mosquitoes has been observed for mosquitoes previously infected with la crosse (lac) virus. by contrast, prior infection of mosquitoes with lac virus did not interfere significantly with the subsequent infection and replication of guaroa bunyavirus (bunyamwera serogroup), or heterologous viruses such as west nile flavivirus, or vesicular stomatitis rhabdovirus. | 1983 | 6305019 |