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sindbis and middelburg old world alphaviruses associated with neurologic disease in horses, south africa.old world alphaviruses were identified in 52 of 623 horses with febrile or neurologic disease in south africa. five of 8 sindbis virus infections were mild; 2 of 3 fatal cases involved co-infections. of 44 middelburg virus infections, 28 caused neurologic disease; 12 were fatal. middelburg virus likely has zoonotic potential.026583836
larval feeding behavior of three co-occurring species of container mosquitoes.interspecific differences in foraging behavior may help to determine whether the outcome of interspecific competition is coexistence or exclusion. mosquitoes in the genus culex are commonly described as foraging primarily by filtering the water column. this behavior contrasts with that of other container-dwelling genera, such as aedes and ochlerotatus, that are thought to forage primarily by browsing on container and detritus surfaces. we compared the feeding behavior of cx. pipiens, ae. albopic ...015707290
identification of mosquito avian-derived blood meals by polymerase chain reaction-heteroduplex analysis.a polymerase chain reaction (pcr) heteroduplex assay (hda) was developed to identify avian derived mosquito blood meals to the species level. the assay used primers amplifying a fragment of the cytochrome b gene from vertebrate but not invertebrate species. in culex tarsalis fed on quail, pcr products derived from the quail cytochrome b gene were detected seven days post-engorgement. in an analysis of wild-caught mosquitoes, 85% of blood-fed mosquitoes produced detectable pcr products. heterodup ...012201598
risk of transmission of viral haemorrhagic fevers and the insecticide susceptibilitystatus of aedes aegypti (linnaeus) in some sites in accra, ghana.dengue is one of the emerging diseases that can mostly only be controlled by vector control since there is no vaccine for the disease. although, dengue has not been reported in ghana, movement of people from neighbouring countries where the disease has been reported can facilitate transmission of the disease.027752187
arbovirus infections.arbovirus (arthropod-borne virus) infections are increasingly important causes of neurologic disease in the united states through both endemic transmission and travel-associated infections. this article reviews the major arbovirus infections that can cause neurologic disease likely to be encountered in the united states.026633778
chikungunya vaccines in development.chikungunya virus has become a global health threat, spreading to the industrial world of europe and the americas; no treatment or prophylactic vaccine is available. since the late 1960s much effort has been put into the development of a vaccine, and several heterogeneous strategies have already been explored. only two candidates have recently qualified to enter clinical phase ii trials, a chikungunya virus-like particle-based vaccine and a recombinant live attenuated measles virus-vectored vacc ...026554522
eastern equine encephalitis treated with intravenous immunoglobulins.we report the case of a 68-year-old man from southeastern massachusetts presenting with encephalitis due to eastern equine encephalitis (eee) virus. despite the high morbidity and mortality rate of eee, the patient made a near complete recovery in the setting of receiving early intravenous immunoglobulins.026740855
inventory of mosquitoes (diptera: culicidae) in conservation units in brazilian tropical dry forests.in brazil, most studies of the culicidae family are concentrated in rainforest regions. as such, there is a lack of knowledge regarding the diversity of culicidae in regions with different climatic and vegetational characteristics. the aim of this study was to compile an inventory of culicidae in protected areas of the semi-arid region of the state of minas gerais, brazil, in order to better understand the diversity of the family within this region. the study was conducted across four protected ...026200963
climate, environmental and socio-economic change: weighing up the balance in vector-borne disease transmission.arguably one of the most important effects of climate change is the potential impact on human health. while this is likely to take many forms, the implications for future transmission of vector-borne diseases (vbds), given their ongoing contribution to global disease burden, are both extremely important and highly uncertain. in part, this is owing not only to data limitations and methodological challenges when integrating climate-driven vbd models and climate change projections, but also, perhap ...025688012
genome analysis of mayaro virus imported to germany from french guiana. 024960052
powassan meningoencephalitis, new york, new york, usa.disease caused by powassan virus (powv), a tick-borne flavivirus, ranges from asymptomatic to severe neurologic compromise and death. two cases of powv meningoencephalitis in new york, usa, highlight diagnostic techniques, neurologic outcomes, and the effect of powv on communities to which it is endemic.023969017
neuroepidemiology and the epidemiology of viral infections of the nervous system.the field of neurovirology will undoubtedly experience evolution and change in the years to come. the epidemiology of viral cns diseases continues to change, and as our understanding of the pathogenesis and pathophysiology associated with viral agents grows, so does our understanding of the behavior of these pathogens among populations. the appearance of viral pathogens in newsettings, new or unrecognized modes of transmission,and the emergence of previously unrecognized pathogens will continue ...025015481
approaches to the treatment of disease induced by chikungunya virus.chikungunya virus, a re-emerging mosquito-borne alphavirus, causes fever, rash and persistent arthralgia/arthritis in humans. severe outbreaks have occurred resulting in infections of millions of people in southeast asia and africa. currently there are no antiviral drugs or vaccines for prevention and treatment of chikungunya infections. herein we report the current status of research on antiviral drugs and vaccines for chikungunya virus infections.024434329
select agent and toxin regulations: beyond the eighth edition of the guide for the care and use of laboratory animals.in the interval between the publication of the seventh and eighth editions of the guide for the care and use of laboratory animals (guide), much has changed with regard to the regulation and funding of highly pathogenic biologic agents and toxins (select agents). funding of research involving highly pathogenic agents has increased dramatically during this time, thus increasing the demand for facilities capable of supporting this work. the eighth edition of the guide briefly mentions select agent ...022776191
serosurveillance of eastern equine encephalitis virus in amphibians and reptiles from alabama, usa.eastern equine encephalitis virus (eeev) is among the most medically important arboviruses in north america, and studies suggest a role for amphibians and reptiles in its transmission cycle. serum samples collected from 351 amphibians and reptiles (27 species) from alabama, usa, were tested for the presence of antibodies against eeev. frogs, turtles, and lizards showed little or no seropositivity, and snakes had high seropositivity rates. most seropositive species were preferred or abundant host ...022403333
biological transmission of arboviruses: reexamination of and new insights into components, mechanisms, and unique traits as well as their evolutionary trends.among animal viruses, arboviruses are unique in that they depend on arthropod vectors for transmission. field research and laboratory investigations related to the three components of this unique mode of transmission, virus, vector, and vertebrate host, have produced an enormous amount of valuable information that may be found in numerous publications. however, despite many reviews on specific viruses, diseases, or interests, a systematic approach to organizing the available information on all f ...016223950
the future of gene therapy.eventually, gene therapy will become a staple of 21st century medicine. but some experts say society will be better served if medical researchers proceed more slowly and prudently.023393464
habitat associations of eastern equine encephalitis transmission in walton county florida.eastern equine encephalitis virus (eeev; family togaviridae, genus alphavirus) a highly pathogenic mosquito-borne virus is endemic to eastern north america. the ecology of eeev in florida differs from that in other parts of the united states; eeev in the northeastern united states is historically associated with freshwater wetlands. no formal test of habitat associations of eeev in florida has been reported. geographical information sciences (gis) was used in conjunction with sentinel chicken ee ...022679885
eastern equine encephalitis in children, massachusetts and new hampshire,usa, 1970-2010.we describe the clinical, laboratory, and radiographic characteristics of 15 cases of eastern equine encephalitis in children during 1970-2010. the most common clinical and laboratory features were fever, headache, seizures, peripheral leukocytosis, and cerebrospinal fluid neutrophilic pleocytosis. radiographic lesions were found in the basal ganglia, thalami, and cerebral cortex. clinical outcomes included severe neurologic deficits in 5 (33%) patients, death of 4 (27%), full recovery of 4 (27% ...023343480
barriers to success: how baculoviruses establish efficient systemic infections.the mechanisms used by baculoviruses to exit the midgut and cause systemic infection of their insect hosts have been debated for decades. after being ingested, baculoviruses reach the midgut, where several host barriers need to be overcome in order to establish successful infection. one of these barriers is the basal lamina, a presumably virus-impermeable extracellular layer secreted by the epithelial cells lining the midgut and trachea. this review discusses new evidence that demonstrates how t ...021300392
development of a multiplexed polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) assay to identify common members of the subgenera culex (culex) and culex (phenacomyia) in guatemala.morphological differentiation of mosquitoes in the subgenera culex (culex) and culex (phenacomyia) in guatemala is difficult, with reliable identification ensured only through examination of larval skins from individually reared specimens and associated male genitalia. we developed a multiplexed polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) assay to identify common cx. (cux.) and cx. (phc.). culex (cux.) chidesteri, cx. (cux.) coronator, cx. (cux.) interrogator, c ...020682869
mapping eastern equine encephalitis virus risk for white-tailed deer in michigan.eastern equine encephalitis (eee) is a mosquito-borne viral disease that is often fatal to humans and horses. some species including white-tailed deer and passerine birds can survive infection with the eee virus (eeev) and develop antibodies that can be detected using laboratory techniques. in this way, collected serum samples from free ranging white-tailed deer can be used to monitor the presence of the virus in ecosystems. this study developed and tested a risk index model designed to predict ...026494931
a risk index model for predicting eastern equine encephalitis virus transmission to horses in florida.a gis-based risk index model was developed to quantify eeev transmission risk to horses in the state of florida. eeev is a highly pathogenic arbovirus that is endemic along the east coast of the united states, and it is generally fatal to both horses and humans. the model evaluates eeev transmission risk at individual raster cells in map on a continuous scale of 0 to 1. the risk index is derived based on local habitat features and the composition and configuration of surrounding land cover types ...024764607
recovery of eastern equine encephalomyelitis virus from brain tissue of human cases of encephalitis in massachusetts. 193817843373
studies on eastern equine encephalomyelitis : iii. intraocular infection with fixed virus in the guinea pig.the behavior of a fixed strain of eastern equine encephalomyelitis virus was studied in guinea pigs after intraocular inoculation. such inoculation concerns the central and not the peripheral nervous system. the susceptibility to intraocular injection lies midway between the highly virulent intracerebral and the quite avirulent peripheral routes. the virus must act for 10 to 13 hours in order to induce a fatal infection. removal of the inoculated eyeball before this interval almost always preven ...193919870871
influence of age on susceptibility and on immune response of mice to eastern equine encephalomyelitis virus.the experiments described in this paper were carried out with the rockefeller institute strain of albino mice and with the eastern strain of the virus of equine encephalomyelitis. 1. the observation was confirmed that with increasing age of mice there occurred a decrease in susceptibility to intraperitoneal injection of active virus; also, the length of incubation period of those which succumbed increased with age. 2. the mice of various age groups which survived an intraperitoneal injection of ...194119871121
neutralization tests with eastern equine encephalomyelitis virus in the chick embryo; a statistical treatment of the results by the probit method. 194818865908
statistical analysis of factors affecting the susceptibility of chick embryos to eastern equine encephalomyelitis virus. 194818912610
a quantitative method for the assay of serum neutralizing antibodies against eastern equine encephalomyelitis virus using the chick embryo. 194818933286
myositis in mice following intramuscular injection of viruses of the mouse encephalomyelitis group and of certain other neurotropic viruses.a study has been made of the local effects following intramuscular injection of various neurotropic viruses. early massive necrosis of muscle fibers accompanied by edema and acute inflammatory reaction is produced by jungeblut's sk virus even in low concentrations. similar but more slowly developing lesions follow the introduction of mouse encephalomyelitis gd-vii and fa strains. strain 4727 (to type) produces inflammatory changes with fibrosis in the intermuscular septa and necrosis of scattere ...194918099166
isolation of eastern equine encephalomyelitis virus from naturally infected monkey (macacus philippinensis). 194918127637
effect of sodium monofluoroacetate on multiplication of eastern equine encephalomyelitis virus. 195212983405
some effects of certain resorcinol derivatives on eastern equine encephalomyelitis virus; factors influencing in vitro screening technic. 195313047404
effects of storage on eastern equine encephalitis virus. 195413167174
transfer of maternal neutralizing antibodies against eastern equine encephalomyelitis virus in birds. 195413201787
studies on the north american arthropod-borne encephalitides. vii. estimation of amount of eastern equine encephalitis virus inoculated by infected aedes aegypti. 195413207100
isolations of eastern equine encephalomyelitis virus from mosquitoes (culiseta melanura) collected in new jersey, 1953. 195413237273
in vitro method for cultivating eastern equine encephalomyelitis virus in teleost embryos. 195413237295
the effect of various chemical agents on eastern equine encephalomyelitis virus. 195613319266
effect of eastern equine encephalomyelitis virus infection on phosphate transfer and modification by cortisone. 195613336076
[secretion of the eastern equine encephalitis virus and occasional incidence of certain contact infections in mice]. 195613361422
eastern equine encephalomyelitis virus isolated from three species of diptera from georgia. 195713408979
the isolation of eastern equine encephalomyelitis virus from brains of sparrows. 195713428624
multiplication of eastern equine encephalitis virus in mosquitoes following intrathoracic inoculation. 195713441704
erratum.in reference 5 of "eastern equine encephalomyelitis virus isolated from three species of diptera from georgia," by l. h. karstad et al. [science 125, 396 (1 mar. 1957)], the name of j. e. mccroan is incorrectly spelled as croan.195717793795
an evaluation of various tissues in culture for isolation of eastern equine encephalitis virus. 195813518206
elimination of the virus of eastern equine encephalomyelitis (eee) in the faeces of experimentally infected ticks ixodes ricinus l. and dermacentor marginatus sulz. 195813594715
[course of infection with the eastern equine encephalomyelitis virus and development of active immunity in grown mice]. 195813625975
[value of intracerebral inoculation of mice & of tissue culture tests for the detection of minimal amounts of eastern equine encephalomyelitis virus]. 195813625991
[immunity of the white mouse to the eastern equine encephalomyelitis virus. i. effect of immune serum in vivo]. 195913648564
[immunity of white mouse to eastern equine encephalomyelitis virus. ii. studies with tissue culture]. 195913648580
eastern equine encephalitis virus isolated from culex nigripalpus in trinidad. 195913817918
isolation of eastern equine encephalomyelitis virus from aedes vexans in connecticut. 196013842647
propagation of eastern equine encephalomyelitis (eee) virus in surviving tick tissues. 196013854482
hemagglutination-inhibition and serum neutralization response of horses to eastern equine encephalomyelitis virus. 196014401538
eastern equine encephalitis virus in the west indies and british guiana. 196113915651
interference with eastern equine encephalomyelitis (eee) virus in the brains of mice immune to lymphocytic choriomeningitis (lcm). 196113922306
mortality patterns and antibody response in chicks inoculated with eastern equine encephalomyelitis virus. 196113689593
cross-protection between venezuelan equine encephalomyelitis and eastern equine encephalomyelitis virus. 196113712597
cross-neutralization studies with group a arthropod-borne viruses.in an extension of recent work on the antigenic interrelationships of arthropod-borne viruses, the plaque-inhibition test has been applied to the study of 15 group a strains. middelburg and eastern equine encephalomyelitis viruses show no relationship to any other virus in the group. sindbis and western equine encephalomyelitis viruses show a one-way relationship only. the remaining viruses all share some antigenic components which react with hyperimmune rabbit sera prepared against semliki fore ...196113737288
biological studies of interferon. i. suppression of cellular infection with eastern equine encephalomyelitis virus. 196113782523
the lizard: a possible host for eastern equine encephalitis virus in panama. 196213881977
infection of interferontreated cells with eastern equine encephalomyelitis virus and its ribonucleic acid. 196214471721
antigenic variants of eastern equine encephalitis virus.a study by hemagglutination-inhibition test showed that 19 strains of eastern equine encephalitis virus grouped themselves in two main types, which have been designated north american and south american. the former consists of ten strains from the eastern half of the united states, from massachusetts to florida; jamaica, the dominican republic, and, subject to confirmation, thailand. the south american type comprises nine strains from panama, trinidad, british guiana, brazil, and argentina. the ...196414151098
potentiating effect of fractions of eastern equine encephalomyelitis virus on interferon production. 196414200098
in vivo antiviral activity of 1,3-bis(2-chloroethyl)-1-nitrosourea.a prolongation in the lives of swiss mice inoculated intracerebrally with lymphocytic choriomeningitis virus (lcm) was observed after treatment with 1,3-bis(2-chloroethyl)-1-nitrosourea (bcnu). a variety of treatment schedules, including therapy once or twice daily up to 17 days and single treatments at various times after virus inoculation, were employed. virus titers ranging to greater than 10(4) were detected in the blood and brains of surviving drug-treated animals. in three comparative stud ...196514339266
observations on the ability of haemogamasus liponyssoides ewing and ornithonyssus bacoti (hirst) (acarina, gamasina) to retain eastern equine encephalitis virus: preliminary report. 19665901621
[a comparative study of various genetic markers of eastern equine encephalomyelitis virus and its mutants induced by nitrous acid]. 19675611003
temperature-sensitive steps in the biosynthesis of venezuelan equine encephalitis virus.in contrast to eastern equine encephalitis virus, the replication of venezuelan equine encephalitis (vee) virus was strongly inhibited at 44 c in chick embryo cells. the inhibited steps were analyzed by shifting the incubating temperatures up or down, and by determining during the shifts the rate and extent of infectious ribonucleic acid (rna) synthesis, intact virus synthesis, and formation of complement-fixing antigen or of antigen detectable by a direct fluorescent-antibody technique. the inh ...19674990034
a simplified method for preparing eastern and western equine encephalomyelitis complement fixing antigens. 196715938267
western equine encephalitis and eastern equine encephalitis virus antigens derived from sucrose-acetone treated chicken embryos. 19685645040
fractionation of eastern equine encephalitis virus by density gradient centrifugation in cscl.when partially purified eastern equine encephalitis (eee) virus was centrifuged to equilibrium in cscl, three virus specific bands were observed. a hemagglutinin was detected at a buoyant density of 1.18 g/cm(3). infectious eee virus banded in two positions; most of the virus banded at 1.20 g/cm(3) and a lesser amount banded at 1.22 to 1.23 g/cm(3). analysis of radioactive profiles of cscl-fractionated eee virus labeled with either (32)po(4) or (3)h-uridine suggested that the hemagglutinin was s ...19685723703
[multiplication of the eastern equine encephalitis virus in primary hamster kidney cells]. 19704925648
altered viral ribonucleic acid synthesis by a temperature-sensitive mutant of eastern equine encephalitis virus. 19705466757
eastern equine encephalitis virus infection: electron microscopic studies of mouse central nervous system. 19705470808
eastern equine encephalomyelitis virus: an electron microscopic study of aedes triseriatus (say) salivary gland infection. 19715543265
identification of two south american strains of eastern equine encephalomyelitis virus from migrant birds captured on the mississippi delta. 19715568603
biological, physical, and chemical properties of eastern equine encephalitis virus. i. purification and physical properties.a new purification procedure was adopted for eastern equine encephalitis virus which does not subject the virus to pelleting at any stage. three- to 4-liter volumes were passed through a diethylaminoethyl cellulose column. the virus-containing fractions were banded on a sucrose cushion and finally concentrated in an isopycnic band in a linear sucrose gradient. this method reduced the volume 1,000-fold with a concomitant increase in viral titer, i.e., better than 90% recovery. numerous criteria h ...19714106780
isolations of venezuelan and eastern equine encephalomyelitis viruses from sentinel hamsters exposed in the pacific lowlands of colombia. 19715088396
effect of interferon on synthesis of eastern equine encephalitis virus rna. 19715104127
production of high titer eastern equine encephalomyelitis virus and viral antigens in chick embryo suspension cultures. 19724551960
polyadenylic acid sequences in the virion rna of poliovirus and eastern equine encephalitis virus.poliovirus virion rna contains a single covalently bound sequence of polyadenylic acid which is approximately 49 nucleotides long. a single, slightly longer polyadenylic acid sequence is contained in eastern equine encephalitis virus rna. since these viruses are otherwise dissimilar these sequences may play a common role in viral replication, possibly in translation of the viral rna.19724338097
[studies on the pathogenesis of eastern equine encephalomyelitis (eee) in the mouse after injections of virus]. 19725050029
[isolation and properties of interferon from chicken embryo fibroblasts and mouse brain infected with the eastern equine encephalomyelitis virus (eee)]. 19725050030
a comparison of clinical manifestations and pathology of the equine encephalidites: vee, wee, eee. 19734534742
genetic characteristics of mutants of eastern equine encephalomyelitis virus induced by alkylating compounds. 19744413560
[mutability of viruses of the equine encephalitis group under the influence of alkylating compounds. i. mutational change of equine encephalitis viruses due to the action of formaldehyde and of n-nitroso-methyl-urea]. 19744465900
[mutability of viruses of the equine encephalitis group under the influence of alkylating compounds. 2. genetic characteristics of mutants of eastern and venezuelan equine encephalitis viruses produced by formaldehyde and by n-nitroso-methyl-urea]. 19744465904
[brain gangliosides: a possible relation between the structure and the property of producing hemagglutination inhibition of the eastern equine encephalitis virus]. 19744467219
eastern equine encephalitis virus. quantitative study of the effects of interferon on virus replication. 19744472077
epidemiological studies on eastern equine encephalitis virus in são paulo, brazil. 19744477404
the mutagenic effect of some alkylating compounds on eastern equine encephalomyelitis virus. 19744846207
the pattern of virus-specific peptide synthesis in rabbit cells infected with eastern equine encephalitis virus and its response to interferon. 19744856417
infection and transmission of eastern equine encephalomyelitis virus with colonized culiseta melanura (coquillett). 19744150912
ecology of keystone virus, a transovarially maintained arbovirus.our studies in the pocomoke cypress swamp of maryland have shown that key strain of ce is endemic and is carried by the floodwater mosquito a. atlanticus. the virus is transmitted transstadially in nature, as evidenced by our recovery of virus from larvae and males of this species. serologic evidence, both here and elsewhere, indicates that vertebrates are infected with key, but their role in the transmission cycle remains unknown. we have found several animals, for example, the gray squirrel, t ...197520017
eastern equine encephalomyelitis in upstate new york 1972-1974. 1975919
attenuation and immunogenicity of ts mutants of eastern encephalitis virus for mice.preliminary experiments were intiated to test the attenuation for mice of chemically induced temperature-sensitive (ets) mutants of a virulents strain of eastern encephalitis (e) virus, and the potential of such mutants as live virus vaccines for mice. the reversion frequencies of eight mutants to temperature insensitivity were measured and the defects in their biosynthesis at the non-permissive temperature were studied. all eight mutants were less virulent for mice, but the extent of avirulence ...197549393
letter: increased potency of killed vee (tc-83) vaccine in mice previously infected with eee virus. 197549960
cellular resistance to arbovirus infection.when an arbovirus enters an arthropod in an infected blood meal, several mechanisms may interact to affect its life cycle and ultimate transmissibility. intrinsic absolute failure in the establishment of infection must be contrasted with infection that is successfully established but is variably modulated in its viral yield throughout the vector's life-span. degrees of vertebrate host resistance make this modulation a central factor in determining whether an arthropod is an important vector in n ...1975801110
isolation of chikungunya virus contaminating an aedes albopictus cell line.an aedes albopictus cell line was found contaminated with structures morphologically compatible with an alphavirus. rapid isolation of a cytopathic virus was effected by combining sonication, concentration with aquacide ii-r, rate zonal sedimentation and subsequent plating of fractions on vero cells under agar overlay. the virus caused neithedeath nor disease on inoculation into infant and adult mice. it produced a c.p.e. in vero and bhk 21 cells, and multiplied in singh's aedes aegypti cells. t ...1975806659
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