Publications

serological relationship between maize rough dwarf virus and rice black-streaked dwarf virus.		1973	4215213
rice black-streaked dwarf virus: its properties, morphology and intracellular localization.		1977	855190
conserved terminal nucleotide sequences in the genome of rice black streaked dwarf virus.	the terminal regions of the dsrna genome segments of rice black streaked dwarf virus (rbsdv) were sequenced. the individual dsrnas, which were 32p-labelled at their 3' termini by incubation with [32p]pcp and t4rna ligase, were separated by 5% page, and the 10 dsrna segments were sequenced by two-dimensional electrophoresis. the common 3'-terminal sequences ---guc 3' and ---aaaaacuu 3' were found in the plus and minus strands, respectively. the strictly conserved terminal sequences (5' aaguuuuu . ...	1992	1607875
close similarity between genome structures of rice black-streaked dwarf and maize rough dwarf viruses.	the complete nucleotide sequences of rice black-streaked dwarf virus (rbsdv) genome segments 8 (s8) and 7 (s7) were determined, and were found to have high sequence identities to the corresponding maize rough dwarf virus (mrdv) genome segments. rbsdv s8 and s7 consisted of 1927 and 2193 nucleotides, respectively. rbsdv s8 had a single long open reading frame (orf) encoding 591 amino acids. rbsdv s7 had two nonoverlapping orfs encoding 362 (orf1) and 309 (orf2) amino acids. the two orfs of rbsdv ...	1993	8336114
nucleotide sequence of nilaparvata lugens reovirus genome segment s8 coding for the major outer capsid protein.	the complete nucleotide sequence of genome segment 8 (s8) of nilaparvata lugens reovirus (nlrv) was determined. it consisted of 1802 nucleotides containing a long open reading frame (562 amino acids), which was expressed in escherichia coli as a fusion protein. the expressed s8 product, a 62k protein, was detected by western blotting using igg directed against intact nlrv particles. this result indicates that s8 encodes the major outer capsid protein of nlrv. the protein exhibited 18.6% amino ac ...	1994	7931170
complete nucleotide sequence of the nilaparvata lugens reovirus: a putative member of the genus fijivirus.	the nucleotide sequences of all genome segments of the nilaparvata lugens reovirus (nlrv), which is found in the brown planthopper nilaparvata lugens, have been determined and some genes have been assigned to structural and functional proteins. the genome of nlrv consists of 28 699 nucleotides and contains at least 11 large open reading frames (orfs). the genome of nlrv is the largest among viruses of the family reoviridae reported to date. the deduced amino acid sequence of genome segment s1 co ...	1996	8558122
taxonomic characteristics of fijiviruses based on nucleotide sequences of the oat sterile dwarf virus genome.	sequence determination of full-length cdna clones of genomic segments 7-10 (s7-s10) of oat sterile dwarf fijivirus (osdv) revealed that the 5' and 3' ends of the plus strands of these segments had the same conserved terminal sequences, 5' aacgaaaaa and uuuuuuuaguc 3'. these sequences are similar, but not identical, to the conserved terminal nucleotide sequences of the genomic segments of rice black streaked dwarf fijivirus (rbsdv) and maize rough dwarf fijivirus (mrdv). the coding strands of s7 ...	1998	9634091
identification of rice black-streaked dwarf fijivirus in maize with rough dwarf disease in china.	three virus isolates from maize with rough dwarf in different provinces in china were analyzed at the molecular level. when compared to an isolate from diseased rice plants in hubei province, all four isolates had identical genomic rna electrophoretic profiles, which were composed of ten double-stranded (ds) rnas. full-length cdnas of segment 10 (s10) from each of the four isolates were cloned by rt-pcr and the complete sequences were determined. analysis of the sequences revealed that each cons ...	2001	11266211
molecular characterisation of segments 1 to 6 of rice black-streaked dwarf virus from china provides the complete genome.	the nucleotide sequences of segments s1 to s6 of a chinese isolate of rice black-streaked dwarf virus (rbsdv) were determined. this provides the first complete sequence of a plant pathogenic member of the genus fijivirus. the complete ten-segment genome has 29,141 nucleotides, making it the largest reovirus genome so far reported. each of the segments s1-s6 is predicted to encode a single major protein. protein comparisons indicated that s1 encoded an rna dependent rna polymerase, with similarit ...	2001	11811683
cloning and sequencing of the genome segment s9 of rice black-streaked dwarf virus.	genome segment s9's of rice black-streaked dwarf virus (rbsdv) of three chinese isolates were amplified by rt-pcr and sequenced, and were found to be consisted of 1 900 nt (rbsdv-zj s9 embl accession number aj297430),1 898 nt (rbsdv-heb s9 embl accession number aj297429) and 1 900 nt (rbsdv-hub s9 embl accession number aj291706), respectively. genome segment s9's of three chinese isolates shared 98.5%--98.8% sequence homology and all contained two open reading frames (orf), which encoded two pol ...	2001	12040425
identification of rice black streaked dwarf virus in different cereal crops with dwarfing symptoms in china.	this report describes isolation of virus particles from plants of rice, maize, wheat and sorghum with symptoms of dwarfing collected from two provinces of china, purification of double-stranded rna (dsrna) from the virus particles, and synthesis of full-length cdnas of genome segments 9 (s9) and 10 (s10) by reverse transcription-polymerase chain reaction (rt-pcr). sequence analysis showed that the s9 sequences of the chinese isolates and a japanese rice black-streaked disease virus (rbsdv) isola ...	2001	12083334
nucleotide sequences of double-stranded rna segments from a hypovirulent strain of the white root rot fungus rosellinia necatrix: possibility of the first member of the reoviridae from fungus.	twelve double-stranded (ds) rna segments were detected from a hypovirulent strain w370 of the white root rot fungus rosellinia necatrix. the estimated molecular weights ranged from 0.41 x 10(6) to 2.95 x 10(6). full length cdna clones for eight segments were obtained. northern blot analysis suggested that each segment was genetically unique. the nucleotide sequences of eight full length dsrna segments were determined. one long open reading frame was found in each segment. conserved sequences at ...	2002	12206302
sequence analysis of genome segments s4 and s8 of mal de río cuarto virus (mrcv): evidence that the virus should be a separate fijivirus species.	this is the first sequence-based characterization of mal de río cuarto virus (mrcv), currently classified as a variant of maize rough dwarf virus (mrdv) and exclusively found in south america. we sequenced and analyzed genome segments s4 and s8. mrcv s4 coded for a putative 131.67 kda protein while mrcv s8 coded for a putative 68.26 kda protein containing an atp/gtp-binding motif. the 5' and 3' ends of mrcv segments, were 5'aaguuuuu3' and 5'cagcunnnguc3', respectively. prediction of secondary st ...	2002	12209310
selection of phage-display peptides that bind specifically to the outer coat protein of rice black streaked dwarf virus.	several peptides that could bind specifically to the outer coat protein encoded by the s10 gene of rice black streaked virus (rbsdv) were isolated from a phage-display random 12-mer peptide library. the sequence analysis showed that the amino acid motif (k)k*()p, the asterisk denoting any amino acid, might be the core sequence by which the peptides bind to the target protein. the peptide 1 that had a high affinity to rbsdv outer coat protein was synthesized by a chemical method and its fusion ...	2002	12387499
phylogenetic analysis reveals that a dwarfing disease on different cereal crops in china is due to rice black streaked dwarf virus (rbsdv).	a viral disease with dwarfing symptoms is associated with severe damage of different cereal crops including rice, maize, wheat and sorghum grown in china. it is believed that the pathogenic agent of the disease on rice and sorghum is rice black streaked dwarf virus (rbsdv), however, the cause of maize dwarf disease in china is still inconclusive. in this report, dsrna was isolated from virus particles obtained from the diseased plants of rice, maize, wheat and sorghum from two chinese provinces. ...	2002	12416683
[cdna cloning and sequence analysis of genome segment s7 of rice black-streaked dwarf virus].	genome segments 7 of zhejiang and hebei isolates of rice black-streaked dwarf virus (rbsdv) were amplified and sequenced. segment 7 of zhejiang isolate was consisted of 2193 nts (embl accession no. aj297427) in length and that of hebei isolate was 2190 nts (aj297428). both segments contained two non-overlapping open reading frames (orfs), which encoded two polypeptides with molecular weights of 41 kd and 36 kd. these two segments shared 99% nucleotide identity, 100% and 94.4% amino acid identiti ...	2002	12557397
sequence and phylogenetic analysis of genome segments s1, s2, s3 and s6 of mal de río cuarto virus, a newly accepted fijivirus species.	mal de río cuarto virus (mrcv) is a newly described species of the genus fijivirus, family reoviridae. the nucleotide sequence of four mrcv genome segments was determined. mrcv s1, s2, s3 and s6 were predicted to encode proteins of 168.4, 134.4, 141.7 and 90 kda, respectively. mrcv s1 encodes a basic protein that contains conserved rna-dependent rna polymerase motifs, and is homologous to rice black streaked dwarf virus (rbsdv), fiji disease virus (fdv) and nilaparvata lugens reovirus (nlrv) pol ...	2003	12606083
molecular analysis of fiji disease fijivirus genome segments 1 and 3.	fiji disease fijivirus (fdv) genomic segments 1 (s1) and 3 (s3) were completely sequenced. fdv s1 comprised 4,532 nt and was predicted to encode a 170.6 kda protein. fdv s3 comprised 3,623 nt and was predicted to encode a 135.5 kda protein. the terminal sequences of s1 and s3 were 5' aaguuuuu......cagcuagcguc 3' and 5' aaguuuuu......cagcagauguc 3', respectively, and located immediately adjacent to these sequences were 12 bp imperfect inverted repeats. the predicted translation product of fdv s1 ...	2003	12876456
sequence analysis of the complete genome of rice black-streaked dwarf virus isolated from maize with rough dwarf disease.	the complete nucleotide sequences of 10 genomic segments (s1-s10) from an isolate of rice black-streaked dwarf virus causing rough dwarf disease on maize (rbsdv-hbm) in china were determined, a total of 29,142 base pairs (bp). each segment possessed the genus-specific termini with conserved nucleotide sequences of (+) 5'-aaguuuuu......cagcunnnguc-3' and a perfect or imperfect inverted repeat of seven to eleven nucleotides immediately adjacent to the terminal conserved sequence. while the coding ...	2003	14501194
[cdna cloning and expression in escherichia coli of rice black-streaked dwarf virus segment 7].	using primers designed from the terminal sequences of maize rough dwarf virus s6, a 2.2 kb cdna fragment was amplified by rt-pcr from maize plants showing maize rough dwarf disease. sequence analysis shows that the full length of this cdna is 2193bp. it contains two open reading frames that encoded two polypeptides with molecular weight of 41.0kd and 36.3kd, respectively. results of multi-sequences alignment suggest that, this cdna sequence has significant similarity to rice black-streaked dwarf ...	2003	16276917
[cloning and expression of s9-1 gene of rice black-streaked dwarf virus in escherichia coli].	the cdna of rbsdv s9 was amplified by rt-pcr using the total rna of infected maize leaves as the template, and then the pcr product was cloned into pbluescriptii sk. after the dna sequence was determined, the s9-1 gene was subcloned into e. coli expression vector pet-21d. sds-page analysis revealed that a 40 kd protein was highly expressed. n-terminal sequence analysis confirmed the 40 kd protein was s9-1 protein. antiserum was prepared using the purified protein. western blotting indicated that ...	2003	16279198
molecular analysis of fiji disease virus genome segments 5, 6, 8 and 10.	the complete sequences of fiji disease virus (fdv) genome segments 5 (s5), s6, s8 and s10 were obtained and comprised 3150 nt, 2831 nt, 1959 nt and 1819 nt, respectively. each segment contained a single orf which encoded putative proteins of 115 kda, 97 kda, 69 kda and 63.0 kda, respectively. the putative amino acid sequences encoded by s5 and s6 contained putative leucine zipper motifs while fdv s5 and s8 each contained an atp-gtp-binding motif. at the amino acid level, fdv s5, s6, s8 and s10 s ...	2004	15045559
sequence analysis of genome segments s5 and s10 of mal de rio cuarto virus (fijivirus, reoviridae).	mal de rio cuarto virus (mrcv) was recently described as a new species of the genus fijivirus, family reoviridae. the nucleotide sequence of two mrcv genome segments was determined. mrcv s5 and s10 were predicted to encode proteins of 106.9 and 63.5 kda respectively. the protein coded by mrcv s5 had 62.8% and 35.7% identity to fijiviruses rbsdv s5 and fdv s5 coded proteins, and contained a rarely reported type-1 c-terminal peroxisomal targeting signal. the protein coded by mrcv s10 had identity ...	2005	15747051
development of an id-elisa for the detection of rice black-streaked dwarf virus in plants.	the rice black-streaked dwarf virus (rbsdv) virion is composed of two layers of capsid proteins and 10 segments of double-stranded genomic rna (s1-s10). due to the fragility of the rbsdv outer capsid, it is very difficult to obtain intact virus particles for preparation of antiserum needed in virus detection. in this work, the major outer capsid protein (p10) encoded by s10 of rbsdv was expressed in escherichia coli cells as a glutathione-s-transferase (gst) fusion protein. after purification of ...	2006	16406134
molecular analysis of fiji disease virus segments 2, 4 and 7 completes the genome sequence.	the complete nucleotide sequences of fiji disease virus (fdv) genome segments s2, s4 and s7 were determined. this now completes the sequencing of all ten dsrna genome segments of the fijivirus type member, fdv, which comprises a total of 29339 nt. fdv s2, s4 and s7 comprised 3820, 3568 and 2194 nt, respectively. s2 and s4 each contained a single open reading frame (orf), which encoded putative proteins of 137 and 133 kda, respectively, while s7 contained two orfs, which encoded putative proteins ...	2006	16525734
sequencing of the bicistronic genome segments s7 and s9 of mal de río cuarto virus (fijivirus, reoviridae) completes the genome of this virus.	the nucleotide sequences of genomic segments s7 and s9 of mal de río cuarto virus (mrcv, fijivirus group ii) have been determined, thus completing the entire genome sequence of the virus. these segments showed a non-overlapping bicistronic structure, as in other members of the genus. mrcv s7 orf-1 had a length of 1086 bp and encoded a 41.5 kda putative polypeptide, whereas mrcv s7 orf-2 had a length of 930 bp and encoded a 36.8 kda putative polypeptide. proteins of 39 and 20.5 kda were predicted ...	2007	17115303
rice black-streaked dwarf virus outer capsid protein p10 has self-interactions and forms oligomeric complexes in solution.	the p10 protein encoded by s10 orf of rice black-streaked dwarf virus (rbsdv) was thought to be the component of outer shell of viral particle. in the present study, p10 has an ability for self-interaction as shown by a gal4 transcription activator-based yeast two-hybrid assay system and further confirmed by in vitro far-western blot analysis. the domain responsible for p10-p10 self-interaction was mapped to the first 230 amino acids at the n-terminal region of the protein. the oligomerization p ...	2007	17442443
rice black-streaked dwarf virus minor core protein p8 is a nuclear dimeric protein and represses transcription in tobacco protoplasts.	virus-encoding nuclear transcriptional regulators play important roles in the viral life cycle. most of these proteins exhibit intrinsic transcriptional activation or repression activity, and are involved in the regulation of the expression of virus genome itself or important cellular genes to facilitate viral replication and inhibit antiviral responses. here, we report that the minor core protein p8 of rice black-streaked dwarf virus, a dsrna virus infecting host plants and insects, is targeted ...	2007	17499245
rice black streaked dwarf virus p9-1, an alpha-helical protein, self-interacts and forms viroplasms in vivo.	replication and assembly of viruses from the family reoviridae are thought to take place in discrete cytoplasmic inclusion bodies, commonly called viral factories or viroplasms. rice black streaked dwarf virus (rbsdv) p9-1, a non-structural protein, has been confirmed to accumulate in these intracellular viroplasms in infected plants and insects. however, little is known about its exact function. in this study, p9-1 of rbsdv-baoding was expressed in escherichia coli as a his-tagged fusion protei ...	2008	18559948
a black-streaked dwarf disease on rice in china is caused by a novel fijivirus.	an isolate of a plant reovirus causing severe stunting and dark leaf symptoms on rice from guangdong, china, was similar in virion morphology and serologically related to rice black-streaked dwarf virus (rbsdv). the electrophoretic profiles of genome segments of the two viruses in agarose or polyacrylamide gel were indistinguishable. the four genome segments of the new isolate corresponding to rbsdv s7-s10 were amplified by ligation rt-pcr and sequenced. the size and organization of each genome ...	2008	18820828
molecular detection of nine rice viruses by a reverse-transcription loop-mediated isothermal amplification assay.	a reverse-transcription loop-mediated isothermal amplification (rt-lamp) assay was established for the detection of nine viruses from infected rice plants, including rice black-streaked dwarf virus (rbsdv), rice dwarf virus (rdv), rice gall dwarf virus (rgdv), rice ragged stunt virus (rrsv), rice transitory yellowing virus (rtyv), rice stripe virus (rsv), rice grassy stunt virus (rgsv), rice tungro spherical virus (rtsv), and rice tungro bacilliform virus (rtbv). virus-specific primer sets were ...	2010	20837064
rice black-streaked dwarf virus p6 self-interacts to form punctate, viroplasm-like structures in the cytoplasm and recruits viroplasm-associated protein p9-1.	rice black-streaked dwarf virus (rbsdv), a member of the genus fijivirus within the family reoviridae, can infect several graminaceous plant species including rice, maize and wheat, and is transmitted by planthoppers. although several rbsdv proteins have been studied in detail, functions of the nonstructural protein p6 are still largely unknown.	2011	21241517
molecular characterization of segments s7 to s10 of a southern rice black-streaked dwarf virus isolate from maize in northern china.	southern rice black-streaked dwarf virus (srbsdv) is a novel fijivirus prevalent in rice in southern and central china, and northern vietnam. its genome has 10 segments of double-stranded rna named s1 to s10 according to their size. an isolate of srbsdv, jni4, was obtained from naturally infected maize plants from ji'ning, shandong province, in the 2008 maize season. segments s7 to s10 of jni4 share nucleotide identities of 72.6%-73.1%, 72.3%-73%, 73.9%-74.5% and 77.3%-79%, respectively, with co ...	2011	21331890
immunity to rice black streaked dwarf virus, a plant reovirus, can be achieved in rice plants by rna silencing against the gene for the viroplasm matrix protein.	the nonstructural protein p9-1 of rice black streaked dwarf virus has been confirmed to accumulate in viroplasms, the putative sites of viral replication, in infected plants and insects. we transformed rice plants by introducing an rna interference construct against the p9-1-encoding gene. the resultant transgenic plants accumulated short interfering rnas specific to the construct. all progenies produced by self-fertilization of these transgenic plants with induced rna interference against the g ...	2011	21621568
the p7-1 protein of southern rice black-streaked dwarf virus, a fijivirus, induces the formation of tubular structures in insect cells.	southern rice black-streaked dwarf virus (srbsdv), an insect- and plant-infecting reovirus of the genus fijivirus, induced the formation of virus-containing tubules in infected plant and insect vector cells. expression of the nonstructural protein p7-1 of srbsdv in insect cells by a recombinant baculovirus resulted in the formation of tubules with dimensions and appearance similar to those found in srbsdv-infected cells. these tubules protruded from the cell surface, supporting the hypothesis th ...	2011	21671041
proteome profile of maize (zea mays l.) leaf tissue at the flowering stage after long-term adjustment to rice black-streaked dwarf virus infection.	maize rough dwarf disease (mrdd) is a viral disease and causes great yield loss. to better understand the effects of mrdd on plant growth and metabolism, comparative proteomic analysis of leaves from virus-infected and normal plants was performed. in order to eliminate the interference of ribulose-1, 5-bisphosphate carboxylase with low-abundance proteins, total proteins were pre-fractionated by 15% peg and the proteins from supernatant and precipitated fractions were analyzed by 2-de, subsequent ...	2011	21708230
Alteration of gene expression profile in maize infected with a double-stranded RNA fijivirus associated with symptom development.	Maize rough dwarf disease caused by Rice black-streaked dwarf virus (RBSDV) is a major viral disease in China. It has been suggested that the viral infection of plants might cause distinct disease symptoms through the inhibition or activation of host gene transcription. We scanned the gene expression profile of RBSDV-infected maize through oligomer-based microarrays to reveal possible expression changes associated with symptom development. Our results demonstrate that various resistance-related ...	2011	21955602
reverse transcription loop-mediated isothermal amplification of rna for sensitive and rapid detection of southern rice black-streaked dwarf virus.	southern rice black-streaked dwarf virus (srbsdv) causes one of the most serious viral diseases of rice in southeast asia. a reverse transcription loop-mediated isothermal amplification (rt-lamp) assay was developed for detection of srbsdv using total rna extracted from rice tissues and the insect pest, white-backed planthopper. the assay was based on a set of four primers matching a total of six sequences in the s9 region of srbsdv genome. presence of the virus could be detected in rt-lamp reac ...	2011	22227615
expression of cytoplasmic incompatibility and host fitness effects in field populations of sogatella furcifera infected with cardinium.	'candidatus cardinium' is a maternally inherited intracellular bacterium that infects a wide range of arthropods and causes diverse reproductive alterations of its arthropod hosts, including cytoplasmic incompatibility (ci), parthenogenesis and feminization. ci is the most common effect and is expressed as a reduction in the number of offspring in crosses between infected males and uninfected females (or females infected with a different bacterial strain). the white-backed planthopper sogatella ...	2012	23356082
the secretory pathway and the actomyosin motility system are required for plasmodesmatal localization of the p7-1 of rice black-streaked dwarf virus.	rice black-streaked dwarf virus (rbsdv), a plant-infecting reovirus (genus fijivirus), generally induces virus-containing tubules in infected cells. the nonstructural protein p7-1, encoded by the first open reading frame of segment 7, is involved in forming the structural matrix of these tubules. in experiments to investigate the subcellular localization of p7-1 in nicotiana benthamiana epidermal cells, fluorescence of p7-1:egfp was observed in the nucleus, cytoplasm and cell periphery, and in p ...	2012	23271163
transcriptome and comparative gene expression analysis of sogatella furcifera (horváth) in response to southern rice black-streaked dwarf virus.	the white backed planthopper (wbph), sogatella furcifera (horváth), causes great damage to many crops by direct feeding or transmitting plant viruses. southern rice black-streaked dwarf virus (srbsdv), transmitted by wbph, has become a great threat to rice production in east asia.	2012	22558400
crystallographic analysis reveals octamerization of viroplasm matrix protein p9-1 of rice black streaked dwarf virus.	the p9-1 protein of rice black streaked dwarf virus accumulates in viroplasm inclusions, which are structures that appear to play an important role in viral morphogenesis and are commonly found in viruses in the family reoviridae. crystallographic analysis of p9-1 revealed structural features that allow the protein to form dimers via hydrophobic interactions. each dimer has carboxy-terminal regions, resembling arms, that extend to neighboring dimers, thereby uniting sets of four dimers via later ...	2012	22072761
methodology for antibody preparation and detection of southern rice black-streaked dwarf virus.	southern rice black-streaked dwarf virus (srbsdv) is a member of the genus fijivirus of the family reoviridae that causes a devastating disease in rice. seven polypeptide fragments of the putative uncharacterized protein (pup) and the p10 proteins of srbsdv were designed, synthesized, and used to immunize rabbits. titers of polyclonal antibodies against the seven peptides were examined using indirect enzyme-linked immunosorbent assay (elisa), and their specificities were investigated using weste ...	2012	22899314
restriction of viral dissemination from the midgut determines incompetence of small brown planthopper as a vector of southern rice black-streaked dwarf virus.	southern rice black-streaked dwarf virus (srbsdv), a fijivirus, is transmitted by the white-backed planthopper in a persistent-propagative manner. in this study, we found that another planthopper species, the small brown planthopper (sbph), could acquire srbsdv but not transmit it. to identify the transmission barrier for srbsdv in sbphs, sequential infection by srbsdv in the organs of sbphs was studied with immunofluorescence for viral antigens. srbsdv initially entered the epithelial cells of ...	2012	22683297
dot immunobinding assay method with chlorophyll removal for the detection of southern rice black-streaked dwarf virus.	southern rice black-streaked dwarf virus (srbsdv), a new virus from fiji, has seriously damaged rice crops in southern china and northern vietnam in recent years. this virus is difficult to diagnose in the early stages of infection, and is very destructive at the late stage. in the present study, a dot immunobinding assay (diba) that has a high sensitivity for diagnosing srbsdv was developed. two kinds of treatment for the diba were evaluated to determine the most effective one for removing chlo ...	2012	22669043
mapping quantitative trait loci conferring resistance to rice black-streaked virus in maize (zea mays l.).	maize rough dwarf disease (mrdd) is one of the most serious virus diseases of maize worldwide, and it causes great reduction of maize production. in china, the pathogen was shown to be rice black-streaked virus (rbsdv). currently, mrdd has spread broadly and leads to significant loss in china. however, there has been little research devoted to this disease. our aims were to identify the markers and loci underlying resistance to this virus disease. in this study, segregation populations were cons ...	2012	22562145
molecular variation and recombination in rna segment 10 of rice black-streaked dwarf virus isolated from china during 2007-2010.	rice black-streaked dwarf virus (rbsdv) is the causal agent of rice black-streaked dwarf and maize rough dwarf diseases in china. the only open reading frame encoding the viral outer capsid protein on s10 rna of 21 rbsdv isolates was sequenced for phylogenetic and recombination analysis. four natural recombinants were detected, and the recombinant breakpoints were identified. in addition, the distribution of synonymous and non-synonymous nucleotide changes revealed that the virus had been evolvi ...	2012	22447103
development of an insect vector cell culture and rna interference system to investigate the functional role of fijivirus replication protein.	an in vitro culture system of primary cells from white-backed planthopper, an insect vector of southern rice black-streaked dwarf virus (srbsdv), a fijivirus, was established to study replication of the virus. viroplasms, putative sites of viral replication, contained the nonstructural viral protein p9-1, viral rna, outer-capsid proteins, and viral particles in virus-infected cultured insect vector cells, as revealed by transmission electron and confocal microscopy. formation of viroplasm-like s ...	2012	22398296
the development and application of a dot-elisa assay for diagnosis of southern rice black-streaked dwarf disease in the field.	outbreaks of the southern rice black-streaked dwarf virus (srbsdv) have caused significant crop losses in southern china in recent years, especially in 2010. there are no effective, quick and practicable methods for the diagnosis of rice dwarf disease that can be used in the field. traditional reverse transcription-polymerase chain reaction (rt-pcr) methodology is accurate but requires expensive reagents and instruments, as well as complex procedures that limit its applicability for field tests. ...	2012	22355457
screening anti-southern rice black-streaked dwarf virus drugs based on s7-1 gene expression in rice suspension cells.	southern rice black-streaked dwarf virus (srbsdv) is a rice pathogen that had an outbreak in southern china in 2010 and caused significant crop losses. therefore, screening for effective antiviral drugs against srbsdv is very important. this study used rice suspension cells infected with srbsdv by polyethylene glycol-mediated uptake for screening antiviral drugs. srbsdv p7-1, which is coded by the s7-1 gene, has an intrinsic ability to self-interact to form tubules that play an important role in ...	2013	23915352
one-step multiplex reverse transcription-polymerase chain reaction for the simultaneous detection of three rice viruses.	rice stripe virus (rsv), rice black-streaked dwarf virus (rbsdv), and rice dwarf virus (rdv) are major rice-infecting viruses in korea that can cause serious crop losses. a one-step multiplex reverse transcription-polymerase chain reaction (mrt-pcr) was developed for the simultaneous detection of these rice viruses. three sets of specific primers targeted to the capsid protein coding genes of rsv, rbsdv, and rdv were used to amplify fragments that were 703 bp, 485 bp, and 252 bp, respectively. t ...	2013	23850700
genetic structure of rice black-streaked dwarf virus populations in china.	rice black-streaked dwarf virus (rbsdv) is a double-stranded rna (dsrna) virus belonging to the genus fijivirus in the family reoviridae. the genome of rbsdv consists of ten dsrna segments. although rbsdv has caused significant economic losses to rice and maize production in the past few years in china, its molecular diversity and evolution remain largely unknown. to elucidate the factor(s) underlying the evolution of rbsdv, we determined segment 8 (s8; carrying orf8 encoding the minor core caps ...	2013	23807744
characterization of rice black-streaked dwarf virus- and rice stripe virus-derived sirnas in singly and doubly infected insect vector laodelphax striatellus.	replication of rna viruses in insect cells triggers an antiviral defense that is mediated by rna interference (rnai) which generates viral-derived small interfering rnas (sirnas). however, it is not known whether an antiviral rnai response is also induced in insects by reoviruses, whose double-stranded rna genome replication is thought to occur within core particles. deep sequencing of small rnas showed that when the small brown planthopper (laodelphax striatellus) was infected by rice black-str ...	2013	23776591
effects of southern rice black-streaked dwarf virus on the development and fecundity of its vector, sogatella furcifera.	southern rice black-streaked dwarf virus (srbsdv) threatens rice production in china and vietnam. the virus is vectored by the migrating white-backed planthopper (wbph, sogatella furcifera) in a circulative, propagative, and persistent manner. a persistently-transmitted plant virus might affect its vector's development and fecundity directly by infecting the vector itself and/or indirectly altering the host plant. this study evaluated the direct and indirect effects of srbsdv on wbph performance ...	2013	23663428
non-structural protein p6 encoded by rice black-streaked dwarf virus is recruited to viral inclusion bodies by binding to the viroplasm matrix protein p9-1.	like other members of the family reoviridae, rice black-streaked dwarf virus (rbsdv, genus fijivirus) is thought to replicate and assemble within cytoplasmic viral inclusion bodies, commonly called viroplasms. rbsdv p9-1 is the key protein for the formation of viroplasms, but little is known about the other proteins of the viroplasm or the molecular interactions amongst its components. rbsdv non-structural proteins were screened for their association with p9-1 using a co-immunoprecipitation assa ...	2013	23636822
quantitative detection of relative expression levels of the whole genome of southern rice black-streaked dwarf virus and its replication in different hosts.	in recent years, a disease caused by southern rice black-streaked dwarf virus (srbsdv) has resulted in significant loss in rice production in southern china and has spread quickly throughout east and southeast asia. this virus is transmitted by an insect vector, white-backed planthopper (wbph) sogatella furcifera (hemiptera: delphacidae), in a persistent propagative manner. aside from rice, srbsdv can also infect numerous poaceae plants. however, the molecular mechanism of interaction between sr ...	2013	23631705
evidence of recombination and genetic diversity in southern rice black-streaked dwarf virus.	southern rice black-streaked dwarf virus (srbsdv) causes one of the most serious viral diseases of rice in china and vietnam. sequence identities of s10, encoding the major capsid protein, were 98.0 %-100 % and 98.3 %-100 % at the nucleotide and amino acid level, respectively. our results suggest that the codon at position 550 of s10 is under positive selection, while most of the other codons are under neutral evolution. putative recombination events were identified in genomic rna segments s1, 2 ...	2013	23605668
identification and characterization of the interaction between viroplasm-associated proteins from two different plant-infecting reoviruses and eef-1a of rice.	a rice protein homologous to eukaryotic translation elongation factor 1a (eef-1a) was found to interact with the pns6 of rice ragged stunt virus (rrsv), the type member of the genus oryzavirus, family reoviridae, in yeast two-hybrid screening. the interaction between the rice protein, designated oseef-1a, and rrsv pns6 was confirmed by bimolecular fluorescence complementation. besides pns6, oseef-1a also interacted with the viroplasm matrix protein, pns10, of rrsv. when expressed together, oseef ...	2013	23605590
new model for the genesis and maturation of viroplasms induced by fijiviruses in insect vector cells.	plant reoviruses are thought to replicate and assemble within cytoplasmic, nonmembranous structures called viroplasms. here, we established continuous cell cultures of the white-backed planthopper (sogatella furcifera horváth) to investigate the mechanisms for the genesis and maturation of the viroplasm induced by southern rice black-streaked dwarf virus (srbsdv), a fijivirus in the family reoviridae, during infection of its insect vector. electron and confocal microscopy revealed that the virop ...	2013	23576499
development and use of three monoclonal antibodies for the detection of rice black-streaked dwarf virus in field plants and planthopper vectors.	rice black-streaked dwarf virus (rbsdv) causes great losses in rice, maize and wheat production in asian countries. the use of serological methods for rbsdv detection depends on the availability of antibodies. in this study, three highly sensitive and specific murine monoclonal antibodies (mabs) against rbsdv antigens were produced using crude extracts from tumors of rbsdv-infected maize as the immunogen, and two serological assays, antigen-coated-plate enzyme-linked immunosorbent assay (acp-eli ...	2013	23575411
interactions between the p6 and p5-1 proteins of southern rice black-streaked dwarf fijivirus in yeast and plant cells.	southern rice black-streaked dwarf virus (srbsdv) is a recently described member of the genus fijivirus, family reoviridae. the roles of the proteins encoded by the srbsdv genome have rarely been studied. in a yeast two-hybrid (yth) assay in which srbsdv p6, a putatively multifunctional protein, was used as bait and an srbsdv cdna library was used as prey, there was a strong interaction between the p6 and p5-1 proteins. the interaction was confirmed by bimolecular fluorescence complement (bifc) ...	2013	23474918
dynamics of southern rice black-streaked dwarf virus in rice and implication for virus acquisition.	a novel viral disease of rice caused by southern rice black-streaked dwarf virus (srbsdv) has spread throughout east and southeast asia since the mid-2000s. outbreaks of this viral disease occur yearly in southern parts of japan concurrently with overseas migration of the planthopper vector sogatella furcifera from southern china during the rainy season (from late june to early july). we examined the dynamics (changes in titer and localization) of srbsdv on rice using reverse-transcription real- ...	2013	23301813
comparative proteomic analysis reveals the cross-talk between the responses induced by h2o2 and by long-term rice black-streaked dwarf virus infection in rice.	hydrogen peroxide (h2o2) could be produced during the plant-virus compatible interaction. however, the cell responses regulated by the enhanced h2o2 in virus infected plant are largely unknown. to make clear the influence of rice black-streaked dwarf virus (rbsdv) infection on h2o2 accumulation, we measured the content of h2o2 and found the h2o2 level was increased in rice seedlings inoculated with rbsdv. to reveal the responses initiated by the enhanced h2o2 during plant-virus interaction, the ...	2013	24312331
migration of rice planthoppers and their vectored re-emerging and novel rice viruses in east asia.	this review examines recent studies of the migration of three rice planthoppers, laodelphax striatellus, sogatella furcifera, and nilaparvata lugens, in east asia. laodelphax striatellus has recently broken out in jiangsu province, eastern china. the population density in the province started to increase in the early 2000s and peaked in 2004. in 2005, rice stripe virus (rsv) viruliferous rate of l. striatellus peaked at 31.3%. since then, rice stripe disease spread severely across the whole prov ...	2013	24312081
overexpression of rice black-streaked dwarf virus p7-1 in arabidopsis results in male sterility due to non-dehiscent anthers.	rice black-streaked dwarf virus (rbsdv), a member of the genus fijivirus in the family reoviridae, is propagatively transmitted by the small brown planthopper (laodelphax striatellus fallén). rbsdv causes rice black-streaked dwarf and maize rough dwarf diseases, which lead to severe yield losses in crops in china. although several rbsdv proteins have been studied in detail, the functions of the nonstructural protein p7-1 are still largely unknown. to investigate the role of the p7-1 protein in v ...	2013	24260239
rice black-streaked dwarf virus p10 induces membranous structures at the er and elicits the unfolded protein response in nicotiana benthamiana.	endoplasmic reticular (er) membrane modifications play an important role in viral rna replication and virion assembly but little is known about the involvement of er-membrane remodeling in the infection cycle of fijiviruses in plant cells. the subcellular localization of rice black-streaked dwarf virus outer capsid p10 was therefore examined using live-cell imaging. p10 fused to egfp formed vesicular structures associated with er membranes in nicotiana benthamiana epidermal cells and in rice pro ...	2013	24210107
nonstructural protein p7-2 encoded by rice black-streaked dwarf virus interacts with skp1, a core subunit of scf ubiquitin ligase.	rice black-streaked dwarf virus (rbsdv), a member of the genus fijivirus within the family reoviridae, causes severe damage to cereal crops in south east asia. the protein p7-2, encoded by the second open reading frame of segment s7, is conserved among most plant-infecting fijiviruses, but its function is still obscure.	2013	24176102
distribution and genetic diversity of southern rice black-streaked dwarf virus in china.	rice and maize dwarf diseases caused by the newly introduced southern rice black-streaked dwarf virus (srbsdv) have led to severe economic losses in south china in recent years. the distribution and diversity of srbsdv have not been investigated in the main rice and maize growing areas in china. in this study, the distribution of srbsdv in china was determined by using reverse transcription-polymerase chain reaction (rt-pcr).	2013	24131521
viroplasm protein p9-1 of rice black-streaked dwarf virus preferentially binds to single-stranded rna in its octamer form, and the central interior structure formed by this octamer constitutes the major rna binding site.	the p9-1 protein of rice black-streaked dwarf virus (rbsdv) is an essential part of the viroplasm. however, little is known about its nature or biological function in the viroplasm. in this study, the structure and function of p9-1 were analyzed for in vitro binding to nucleic acids. we found that the p9-1 protein preferentially bound to single-stranded versus double-stranded nucleic acids; however, the protein displayed no preference for rbsdv versus non-rbsdv single-stranded ssrna (ssrna). a g ...	2013	24067964
southern rice black-streaked dwarf virus: a white-backed planthopper-transmitted fijivirus threatening rice production in asia.	southern rice black-streaked dwarf virus (srbsdv), a non-enveloped icosahedral virus with a genome of 10 double-stranded rna segments, is a novel species in the genus fijivirus (family reoviridae) first recognized in 2008. rice plants infected with this virus exhibit symptoms similar to those caused by rice black-streaked dwarf virus. since 2009, the virus has rapidly spread and caused serious rice losses in east and southeast asia. significant progress has been made in recent years in understan ...	2013	24058362
selection of reference genes for gene expression studies in virus-infected monocots using quantitative real-time pcr.	both genome-wide transcriptomic surveys of the mrna expression profiles and virus-induced gene silencing-based molecular studies of target gene during virus-plant interaction involve the precise estimation of the transcript abundance. quantitative real-time pcr (qpcr) is the most widely adopted technique for mrna quantification. in order to obtain reliable quantification of transcripts, identification of the best reference genes forms the basis of the preliminary work. nevertheless, the stabilit ...	2013	23954326
simultaneous detection and differentiation of rice black streaked dwarf virus (rbsdv) and southern rice black streaked dwarf virus (srbsdv) by duplex real time rt-pcr.	the diseases caused by rice black streaked dwarf virus (rbsdv) and southern rice black streaked dwarf virus (srbsdv) have been occurring epidemically in china and southeastern asia in recent years. a sensitive, reliable and quantitative method is required to detect and distinguish for rbsdv and srbsdv in rice and vector insects.	2013	23331990
enhanced virus resistance in transgenic maize expressing a dsrna-specific endoribonuclease gene from e. coli.	maize rough dwarf disease (mrdd), caused by several fijiviruses in the family reoviridae, is a global disease that is responsible for substantial yield losses in maize. although some maize germplasm have low levels of polygenic resistance to mrdd, highly resistant cultivated varieties are not available for agronomic field production in china. in this work, we have generated transgenic maize lines that constitutively express rnc70, a mutant e. coli dsrna-specific endoribonuclease gene. transgenic ...	2013	23593318
identification and fine-mapping of a qtl, qmrdd1, that confers recessive resistance to maize rough dwarf disease.	maize rough dwarf disease (mrdd) is a devastating viral disease that results in considerable yield losses worldwide. three major strains of virus cause mrdd, including maize rough dwarf virus in europe, mal de río cuarto virus in south america, and rice black-streaked dwarf virus in east asia. these viral pathogens belong to the genus fijivirus in the family reoviridae. resistance against mrdd is a complex trait that involves a number of quantitative trait loci (qtl). the primary approach used t ...	2013	24079304
identification and profiling of conserved and novel micrornas in laodelphax striatellus in response to rice black-streaked dwarf virus (rbsdv) infection.	micrornas (mirnas) are small non-coding endogenous rna molecules that play important roles in various biological processes. this study examined microrna profiles of laodelphax striatellus using the small rna libraries derived from virus free (vf) and rice black-streaked dwarf virus (rbsdv) infected (rb) insects. a total of 59 mature mirnas (46 mirna families) were identified as conserved insect mirnas in both vf and rb libraries. among these conserved mirnas, 24 were derived from the two arms of ...	2014	26484150
southern rice black-streaked dwarf virus alters insect vectors' host orientation preferences to enhance spread and increase rice ragged stunt virus co-infection.	in recent years, southern rice black-streaked dwarf virus (srbsdv), a tentative species in the genus fijivirus (family reoviridae), has spread rapidly and caused serious rice losses in eastern and southeastern asia. with this virus spread, rice ragged stunt virus (rrsv, genus oryzavirus, family reoviridae) became more common in southern china, usually in co-infection with the former. srbsdv and rrsv are transmitted by two different species of planthoppers, white-backed planthopper (wbph, sogatel ...	2014	24047253
rice black-streaked dwarf virus genome segment s5 is a bicistronic mrna in infected plants.	rice black-streaked dwarf virus (rbsdv) is a recognized member of the genus fijivirus, family reoviridae. genome segment s5 has a putative second orf partially overlapping the major orf but in a different reading frame. this putative orf is present in a published sequence and in two chinese isolates now sequenced. antibodies were raised against purified p5-1 and p5-2 fusion proteins expressed in a prokaryotic system. in western blots, these antibodies reacted with proteins of about 106 and 27 kd ...	2014	24013236
influence of rice black streaked dwarf virus on the ecological fitness of non-vector planthopper nilaparvata lugens (hemiptera: delphacidae).	rice black streak dwarf virus (rbsdv) is transmitted by the small brown planthopper (sbph), laodelphax striatellus (fallen). non-vector rice brown planthopper (bph), nilaparvata lugens (stål), shares the same host rice plants with sbph in paddy fields. the changes in nutritional composition of rice plants infected by rbsdv and the ecological fitness of bph feeding on the infected plants were studied under both artificial climate chamber and field conditions. contents of 16 detected amino acids a ...	2014	23956237
infection of rice plants by rice black streaked dwarf virus improves an egg parasitoid, anagrus nilaparvatae (hymenoptera: mymaridae), of rice planthoppers.	the effects of rice plants infected by rice black streaked dwarf virus (rbsdv) on the host preference, duration of immature stages, sex ratio, and adult longevity and parasitic capacity of an egg parasitoid, anagrus nilaparvatae pang et wang, of rice brown planthopper, nilaparvata lugens stål, were evaluated. tests of response to plant volatiles using an olfactometer showed that a. nilaparvatae preferred rice plants harboring rice brown planthopper eggs over plants free of rice brown planthopper ...	2014	25199055
virus-mediated chemical changes in rice plants impact the relationship between non-vector planthopper nilaparvata lugens stål and its egg parasitoid anagrus nilaparvatae pang et wang.	in order to clarify the impacts of southern rice black-streaked dwarf virus (srbsdv) infection on rice plants, rice planthoppers and natural enemies, differences in nutrients and volatile secondary metabolites between infected and healthy rice plants were examined. furthermore, the impacts of virus-mediated changes in plants on the population growth of non-vector brown planthopper (bph), nilaparvata lugens, and the selectivity and parasitic capability of planthopper egg parasitoid anagrus nilapa ...	2014	25141278
a novel, in vivo, indoor method to preserve rice black-streaked dwarf virus in small brown planthopper using wheat seedling as a bridge host.	rice black-streaked dwarf virus (rbsdv) naturally infects gramineae plants through small brown planthopper (sbph) as a vector. however, rbsdv cannot be transmitted to the sbph offspring through transovarian transmission. wheat plant, an important intermediate host in winter, is essential for the completion of the annual cycle of rbsdv in farm ecosystem. we developed a novel, in vivo, indoor method to preserve rbsdv in sbph using wheat seedling as a bridge host. the temperature range of 23-27°c w ...	2014	25075933
micrornas responding to southern rice black-streaked dwarf virus infection and their target genes associated with symptom development in rice.	southern rice black-streaked dwarf virus (srbsdv) is a recently emerged rice virus that has spread across asia. this devastating virus causes rice plants to produce a variety of symptoms during different growth stages. micrornas (mirnas) comprise a large group of 21-24-nt rna molecules that are important regulators of plant development processes and stress responses. in this study, we used microarray profiling to investigate rice mirnas responding to srbsdv infection at 3, 9, 15, and 20 days pos ...	2014	25038403
tumours induced by a plant virus are derived from vascular tissue and have multiple intercellular gateways that facilitate virus movement.	structural studies showed that tumours induced by southern rice black-streaked dwarf virus (srbsdv; genus fijivirus, family reoviridae) were highly organized, modified phloem, composed of sclerenchyma, vessels, hyperplastic phloem parenchyma and sieve elements (ses). only parenchyma and ses were invaded by the virus. there was a special region that consisted exclusively of ses without the usual companion cells and a new flexible type of intercellular gateway was observed on all se-se interfaces ...	2014	24987015
virus-induced tubule: a vehicle for rapid spread of virions through basal lamina from midgut epithelium in the insect vector.	the plant reoviruses, plant rhabdoviruses, tospoviruses, and tenuiviruses are transmitted by insect vectors in a persistent propagative manner. these viruses induce the formation of viral inclusions to facilitate viral propagation in insect vectors. the intestines of insect vectors are formed by epithelial cells that lie on the noncellular basal lamina surrounded by visceral muscle tissue. here, we demonstrate that a recently identified plant reovirus, southern rice black-streaked dwarf virus (s ...	2014	24965461
synergism between southern rice black-streaked dwarf virus and rice ragged stunt virus enhances their insect vector acquisition.	southern rice black-streaked dwarf virus (srbsdv), a tentative species in the genus fijivirus, family reoviridae, is a novel rice virus transmitted by the white-backed planthopper (sogatella furcifera). since its discovery in 2001, srbsdv has spread rapidly throughout eastern and southeastern asia and caused large rice losses in china and vietnam. rice ragged stunt virus (rrsv) (genus oryzavirus, family reoviridae) is a common rice virus vectored by the brown planthopper (nilaparvata lugens). rr ...	2014	24915431
southern rice black-streaked dwarf virus infection improves host suitability for its insect vector, sogatella furcifera (hemiptera: delphacidae).	the impact of southern rice black-streaked dwarf virus (srbsdv) on the life cycle of the vector sogatella furcifera (horváth) (hemiptera: delphacidae) was investigated. the adult longevity was significantly shorter when the insect was reared on the srbsdv-infected rice plants than those reared on the noninfected rice treatment. the fecundity of s. furcifera reared on the srbsdv-infected rice plants was slightly higher than that of those reared on the noninfected rice plants both in macropterous ...	2014	24665689
southern rice black-streaked dwarf virus (srbsdv) directly affects the feeding and reproduction behavior of its vector, sogatella furcifera (horváth) (hemiptera: delphacidae).	southern rice black-streaked dwarf virus (srbsdv) is a recently discovered member of the genus fijivirus and it is transmitted by the rice whitebacked planthopper (wbph), sogatella furcifera (horváth). it was found that srbsdv infected vectors might contribute negatively to the wbph population, although the longer nymphal period might benefit viral acquisition, transmission and increase infection rate. the interaction between srbsdv and its vector need to be further explored to gain better under ...	2014	24661747
proteomic analysis of interaction between p7-1 of southern rice black-streaked dwarf virus and the insect vector reveals diverse insect proteins involved in successful transmission.	southern rice black-streaked dwarf virus (srbsdv), transmitted by the white-backed planthopper (sogatella furcifera) in a persistent-propagative manner, has caused serious yield losses in asia. here in a yeast two-hybrid system, protein interactions between srbsdv p7-1 as a bait protein and a cdna library of s. furcifera as prey protein were assessed. of 153 proteins identified as putative interactors, 24 were selected for further analysis. of the 24 proteins, 18 were further confirmed in a chem ...	2014	24650428
interactive effects of southern rice black-streaked dwarf virus infection of host plant and vector on performance of the vector, sogatella furcifera (homoptera: delphacidae).	performance of insect vectors can be influenced by the viruses they transmit, either directly by infection of the vectors or indirectly via infection of the host plants. southern rice black-streaked dwarf virus (srbsdv) is a propagative virus transmitted by the white-backed planthopper, sogatella furcifera (hovath). to elucidate the influence of srbsdv on the performance of white-backed planthopper, life parameters of viruliferous and nonviruliferous white-backed planthopper fed rice seedlings i ...	2014	26309259
development of proteomic technology of shotgun and label free combined with multiple reaction monitoring to simultaneously detect southern rice black-streaked dwarf virus and rice ragged stunt virus.	the co-infection of rice caused by southern rice black-streaked dwarf virus (srbsdv) and rice ragged stunt virus (rrsv) was widely found at many regions, such as yunnan province, china, and north and central vietnam. these rice viral diseases lead to seriously yield loss of rice. in this study, the proteomics technology of shotgun and label free combined with multiple reaction monitoring (mrm) was developed to detect rice sample of a single or/and co-infection. the shotgun assay indicated that s ...	2014	25674599
phylogenetic and recombination analysis of rice black-streaked dwarf virus segment 9 in china.	rice black-streaked dwarf virus (rbsdv) is an economically important virus that causes maize rough dwarf disease and rice black-streaked dwarf disease in east asia. to study rbsdv variation and recombination, we examined the segment 9 (s9) sequences of 49 rbsdv isolates from maize and rice in china. three s9 recombinants were detected in baoding, jinan, and jining, china. phylogenetic analysis showed that chinese rbsdv isolates could be classified into two groups based on their s9 sequences, reg ...	2015	25633210
genome-wide characterization of rice black streaked dwarf virus-responsive micrornas in rice leaves and roots by small rna and degradome sequencing.	micrornas (mirnas) are small, non-coding rnas which typically function by guiding cleavage of target mrnas. they play important roles in development, abiotic stress and responses to pathogens. four small rna libraries and four degradome libraries were constructed from the leaves and roots of healthy rice and plants infected with rice black streaked dwarf virus (rbsdv). analysis of the deep sequencing results showed that the expression patterns of 14 mirnas in leaves and 16 mirnas in roots change ...	2015	25535197
a simplified method for simultaneous detection of rice stripe virus and rice black-streaked dwarf virus in insect vector.	rice stripe virus (rsv) and rice black-streaked dwarf virus (rbsdv) are transmitted by their common vector small brown planthopper (sbph) that cause serious crop losses in china. a simple reverse transcription-pcr method was developed for the simultaneous detection of rsv and rbsdv in single sbph. three primers targeted to rsv-rna4 and rbsdv-s2 segments were designed to amplify respectively 1114-bp and 414-bp fragments in a reaction. the method is reliable, rapid and inexpensive for detecting th ...	2015	25455902
characterization of homologous and heterologous interactions between viroplasm proteins p6 and p9-1 of the fijivirus southern rice black-streaked dwarf virus.	p6 of southern rice black-streaked dwarf virus (srbsdv) is a multifunctional protein that is involved in the formation of viroplasms by interacting with p5-1. here, we used yeast two-hybrid and bimolecular fluorescence complementation assays to show that there were homologous and heterologous interactions between srbsdv p6 and p9-1 in yeast and plant cells. mutational analysis showed that the n-terminal region (residues 1-93) of p6 was necessary for the interaction between p6 and p9-1. self-inte ...	2015	25377635
assessment of reference gene stability in rice stripe virus and rice black streaked dwarf virus infection rice by quantitative real-time pcr.	stably expressed reference gene(s) normalization is important for the understanding of gene expression patterns by quantitative real-time pcr (rt-qpcr), particularly for rice stripe virus (rsv) and rice black streaked dwarf virus (rbsdv) that caused seriously damage on rice plants in china and southeast asia.	2015	26497487
relation between the viral load accumulation of southern rice black-streaked dwarf virus and the different developmental stages of sogatella furcifera (hemiptera: delphacidae).	the white-backed planthopper, sogatella furcifera (horvath), is currently the only confirmed vector of southern rice black-streaked dwarf virus (srbsdv), which causes severe rice production losses in china. in this study, an absolute quantification qpcr method was used to detect viral gene mrna expression levels at different developmental stages of white-backed planthoppers fed srbsdv-infected rice plants. a comparison of viral copy numbers of the srbsdv s10 gene at the same developmental stage ...	2015	26470211
nac transcription factor family genes are differentially expressed in rice during infections with rice dwarf virus, rice black-streaked dwarf virus, rice grassy stunt virus, rice ragged stunt virus, and rice transitory yellowing virus.	expression levels of the nac gene family were studied in rice infected with rice dwarf virus (rdv), rice black-streaked dwarf virus (rbsdv), rice grassy stunt virus (rgsv), rice ragged stunt virus (rrsv), and rice transitory yellowing virus (rtyv). microarray analysis showed that 75 (68%) osnac genes were differentially regulated during infection with rdv, rbsdv, rgsv, and rrsv compared with the control. the number of osnac genes up-regulated was highest during rgsv infection, while the lowest n ...	2015	26442000
identification and expression profiling analysis of calmodulin-binding transcription activator genes in maize (zea mays l.) under abiotic and biotic stresses.	the calmodulin-binding transcription activators (camta) play critical roles in plant growth and responses to environmental stimuli. however, how camtas function in responses to abiotic and biotic stresses in maize (zea mays l.) is largely unknown. in this study, we first identified all the camta homologous genes in the whole genome of maize. the results showed that nine zmcamta genes showed highly diversified gene structures and tissue-specific expression patterns. many zmcamta genes displayed h ...	2015	26284092
genetic analysis and molecular mapping of qtls for resistance to rice black-streaked dwarf disease in rice.	rice black-streaked dwarf disease, caused by rice black-streaked dwarf virus (rbsdv), is transmitted by small brown planthoppers (laodelphax striatellus fallén, sbph) and causes severe yield loss in epidemic years in china and other east asian countries. breeding for resistance to rbsdv is a promising strategy to control the disease. we identified tetep that showed resistance to rbsdv using a field test and artificial inoculation test. an evaluation of the resistance mechanism revealed that tete ...	2015	26198760
whole-genome expression analysis of rice black-streaked dwarf virus in different plant hosts and small brown planthopper.	rice black-streaked dwarf virus (rbsdv) can infect a number of gramineous plants and cause severe crop yield losses in southeast asian countries. the virus is transmitted by small brown planthopper (sbph) in a persistent circulative manner. the interactions between rbsdv and its different hosts remain unknown. besides, how the virus adjusts itself to infect different hosts is unclear. in the present study, the relative rna levels of the thirteen rbsdv genes in rice, maize, wheat, and sbph were m ...	2015	26149652