Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| the isolation of adenovirus type 1 from a fatal case of viral pneumonitis. | 1958 | 13582270 | |
| isolation of enteric viruses in ontario during 1960-1962. | during the past three years at the central laboratory, ontario department of health, 681 isolations were made in tissue culture from 6822 specimens submitted for virus studies by physicians and hospitals from all over ontario. nearly 74% of the isolates were enteroviruses, approximately 5% adenoviruses and about 1% reoviruses. the remaining 20% are still to be identified.although the bulk of isolations was made during the same three-month period (august, september and october) of each year, the ... | 1963 | 14052975 |
| immunization of volunteers with soluble antigens of adenovirus type 1. | 1964 | 14219941 | |
| [influence of ascorbic acid on viral cytopathogenic activity in tissue culture. 1. study of the effect of ascorbic acid on the cytopathogenic activity of adenovirus type 1]. | 1964 | 5876641 | |
| [experimental infections of cattle with human adenovirus (type 1)]. | 1966 | 4293812 | |
| the presence of adenovirus-specific antigens in hamster cells rendered neoplastic by adenovirus 1-sv40 and adenovirus 2-sv40 hybrid viruses. | 1967 | 4292152 | |
| nutrition and the growth of human adenovirus type 1 in monkey cell cultures: the importance of arginine. | 1969 | 4387636 | |
| transformation of rat embryo cells by adenovirus type 1. | 1969 | 4887234 | |
| the response of bhk21 cells to infection with type 12 adenovirus. 1. cell killing and t antigen synthesis as correlated viral genome functions. | 1969 | 4902254 | |
| the mechanism of viral carcinogenesis by dna mammalian viruses. vi. a new class of virus-specific rna molecules in cells transformed by group c human adenoviruses. | a new class of virus-specific rna molecules was found in cells transformed by group c human adenovirus types 2, 5, and 6. rna isolated from virus-free rat embryo cells transformed by adenovirus 2, 5, and 6 hybridized with all group c adenovirus dna's (adenovirus 1, 2, 5, and 6) equally well, but not appreciably with group a and b adenovirus dna's. most likely no viral genes common to group a, b, and c adenoviruses are transcribed in adenovirus-transformed cells. group c adenoviruses are closely ... | 1969 | 5263008 |
| atypical adenovirus type 1 strains isolated from patients with laryngitis. | 1969 | 5351213 | |
| ultraviolet inactivation of the cytocidal and transforming activities of human adenovirus type 1. | the transforming activity of adenovirus type 1 was more resistant to ultraviolet irradiation than was the cytocidal activity. | 1969 | 5782044 |
| haemagglutinating adeno-associated virus (aav) in association with bovine adenovirus type 1. brief report. | 1970 | 5530958 | |
| rescue of heat-inactivated adenovirus type 1 and 6 by ultraviolet-irradiated adenovirus type 8. | 1970 | 4316777 | |
| isolation of bovine adenovirus type 1 from a calf with pneumo-enteritis. | 1971 | 4333343 | |
| infectivity of human adenovirus-1 dna. | 1972 | 5062855 | |
| [study on adenovirus type 1 infection. 1. virological and serological studies on adenovirus type 1 infection in infant asylum (author's transl)]. | 1973 | 4363430 | |
| [genetic markers of clonal variants of adenovirus type 1]. | 1973 | 4365040 | |
| [study on adenovirus type 1 infection: 1. virological and serological studies on adenovirus type 1 infection in infant asylum (author's transl)]. | 1973 | 4377476 | |
| distribution of antibody to bovine adenovirus type 1 in alabama cattle, as determined by micro-serum-neutralization test. | 1973 | 4707570 | |
| isolation of bovine adenovirus type 1 in the sudan. | 1973 | 4213204 | |
| differentiation of four adenovirus types by macrophage migration inhibition tests. | the macrophage migration inhibition (mmi) test was found to be a satisfactory procedure for distinguishing between adenovirus types 1, 4, 5, and 7. highly purified virus preparations were used for the sensitization of hartley strain guinea pigs, whereas the mmi test antigen consisted of crude virus preparations grown in kb cells. with all four virus types, a significantly greater mmi response was noted when peritoneal exudate cells were exposed to the homologous sensitizing antigen as compared t ... | 1974 | 4426697 |
| canine adenovirus type 2-induced immunity to two canine adenoviruses in pups with maternal antibody. | twenty-four beagle pups with high levels of maternal antibody to canine adenovirus type 1 (cav-1) and canine adenovirus type 2 (cav-2) were oronasally inoculated with cav-2 at 4 weeks of age. the cav-2 was isolated from pharyngeal swabs on postinoculation days 2 through 6. in spite of the infection, maternal antibody continued to decrease for 4 to 8 postinoculation weeks, and then homologous cav-2 neutralizing antibody and, to a lesser extent, cav-1 neutralizing antibody began to increase. when ... | 1975 | 168797 |
| hemagglutinating properties of celo, an oncogenic avian adenovirus. | chicken-embryo-lethal-orphan (celo) virus, phelps strain, agglutinated erythrocytes at 37 c. the hemagglutinating activity, which is a function of complete and incomplete virus particles, was sensitive to heat but not to ph. the soluble components of the virus were similar in sedimentation characteristics to those obtained from human adenovirus type 1. the effects of chemical and physical agents on celo hemagglutinin, celo infectivity, and red-cell receptors suggested that the last were protein ... | 1975 | 173279 |
| viral-antibody complexes in canine adenovirus type 1 (cav-1)ocular lesions: leukocyte chemotaxis and enzyme release. | canine adenovirus-type 1 (cav-1)-antibody complexes caused severe anterior uveitis with corneal edema ("blue eye") when injected into the anterior chamber of normal dogs. the response of the anterior uvea to such immune complexes (ic) was similar to the spontaneously occurring disease. in the presence of complement (c'), ic caused release of neutrophile chemotactic factors. following phagocytosis of ic-c' leukocytes released lysosomal enzymes, as indicated by the presence of acid phosphatase in ... | 1975 | 1095299 |
| corneal endothelium in viral induced anterior uveitis. ultrastructural changes following canine adenovirus type 1 infection. | dogs inoculated intravenously with attenuated canine adenovirus type 1 developed anterior segment inflammation and corneal edema. during the stage of mild anterior uveitis, virus was isolated from the aquious fluid, and by electron microscopy, viral replication was found to occur in corneal endothelial cells. later, at the stage of severe anterior uveitis with corneal edema, virus was not isolated from the aqueous fluid and cells containing intranuclear (replicating) virus were not found. at thi ... | 1975 | 1138688 |
| [the inhibiting action of superhigh-frequency millimeter waves on adenovirus (author's transl)]. | virological and biochemical analysis was employed for comparative studies of the content of protein in continuous cultures of human embryo kidney cells (rh line) infected with adenovirus type 1, intact and irradiated with electromagnetic waves of uhf in the range of 5 divided by 8 mm at the density of power current of the order of 4 mw. when the cells were inoculated with intact virus, a cytopatic effect, a decrease in the content of protein by 4 hours postinfection and further decrease up to 72 ... | 1975 | 1210320 |
| [testing the preventive properties of polyvalent inactivated vaccine (ibr, pi-3, adenovirus 1,3) at a cattle-breeding farm]. | tested was an inactivated polyvalent vaccine. it was shown to produce virus-neutralizing antibodies against the respiratory viruses in high titers. the vaccine proved highly immunogenic, reactogenic, and harmless in doses of 2,3,5,7, and 10 cm3 for 30-45-day-old calves and pregnant cows in their 7th or 8th month of pregnancy. the morbidity rate among the unvaccinated calves ranged from 51 to 66 per cent, and among the vaccinated ones it was from 0 to 5 per cent on the four farms studied. the abo ... | 1976 | 189483 |
| kinetic studies on the cleavage of adenovirus dna by restriction endonuclease eco ri. | the kinetics of cleavage of dna from adenovirus type 1 (ad1), type 5 (ad5) and type 6 (ad6) by restriction endonuclease ecori was investigated by quantitative evaluation of the fluorescence from ethidium stained dna fragments separated on agarose gels. the apparent rate constants of cleavage at different cleavage sites have been determined and large differences in the cleavage rates of the individual sites within one type of dna were found. from the kinetics of cleavage information on the sequen ... | 1976 | 794836 |
| the use of agarose gels for the assay of the deoxyribonuclease activity associated with the soluble penton of adenoviruses. | evidence is presented that the excess pool of penton complexes purified from adenovirus type 1-infected hep-2 cells also carry an endonucleolytic activity. the effect was measured with both e. coli and adenovirus type 1 double-stranded dna substrates. the use of agarose gels for assaying the endonuclease activity allowed the approximative calculation of the number of penton molecules which might produce one dna double-strand break under the experimental conditions selected. | 1976 | 797350 |
| adenovirus type 2 coded single-stranded dna binding protein: in vivo phosphorylation and modification. | the adenovirus type 2-coded single-stranded dna binding protein (dbp) was shown to be a phosphoprotein and to exist in at least two forms that differ in mobility by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. after a 30-min pulse with [35s]methionine or 32po4, 35s- or 32p-labeled dbp had a nominal molecular weight of 74,000 whereas after a 30-min label followed by a 20-h chase, 35s- and 32p-labeled dbp had a nominal molecular weight of 77,000. both large and small forms of 35s- an ... | 1977 | 864833 |
| studies on the different properties of adenovirus penton. | the physical, chemical, immunological and biological properties of the soluble penton antigen of adenovirus strains types 1, 5, 7, 8 and 9, belonging to three distinct subgroups of human adenoviruses, were analysed. mono-, di-, and polymers of the penton were studied by complete and incomplete hemagglutination and a method was developed to separate pentons of subgroup ii. immunological techniques were developed for the rapid detection of penton antigen. elution characteristics of the pentons of ... | 1977 | 867802 |
| comparative studies on the soluble proteins of adenovirus type 1. | the soluble proteins of adenovirus type 1 have been separated and purified. their antigenic characteristics were compared in different precipitation experiments performed in electric field. both two-dimension immune electrophoresis and rocket electrophoresis can successfully be applied for quick diagnostic purposes. quantitative determination of virus proteins is also feasible by rocket electrophoresis. the isoelectric point values of hexon, penton and fibre were pi 4.55, pi 4.69 and pi 7.07, re ... | 1977 | 602822 |
| [group and type antigenic specificity of bovine adenovirus type 1 hexons]. | hexones were obtained from the bovine serotypes 1 and 3. they were investigated through the complement-fixation test along with intact virions in the presence of a hemotype and a heterotype antiserum. it was shown that the free hexones were twice as reactogenic in the homotype system as compared to the heterotype one. the hexones were found to possess two antigenic determinants--a typespecific one and a group-specific one. the latter proved sensitive to chymotrypsin. intact vicions reacted chief ... | 1977 | 76368 |
| [density gradient and ultrastructure of bovine adenovirus type 1]. | isolated and purified were virions and hexones from the bovine adenovirus type 1. the infectious virions were of equilibrium density of 1.34 g/cm3, and the empty capsids--of 1.305 g/cm3. the virions had a facet length of 43.5 nm, their size being 82.7 nm (distance between opposite poles) to 74.0 nm (diameter of the virion). the fibers of the bovine adenovirus type 1 measured 26.0--28.0 nm. the virus was destroyed by 30 per cent formamide, yielding free hexones of 1.290 g/cm3 of equlibrium densit ... | 1978 | 741648 |
| [fragmentation of human adenovirus type 1 dna by the specific endonucleases r. sali, r. ecori and r. bamhi]. | the restricting endonuclease r. ecori hydrolyses human adenovirus type i (adl) dna with formation of three fragments with molecular weights of 17.5 x 10(6), 3.7 x 10(6), and 1.55 x 10(6) dalton. under the effect of r. sali restrictase dna adl disintegrates into 5 fragments with molecular weights: 8.0 x 10(6), 6.0 x 10(6), 4.6 x 10(6), 4.0 x 10(6), and 0.2 x 10(6) dalton. treatment of dna with r. bamhi produces 4 fragments with molecular weights 10.0 x 10(6), 6.8 x 10(6), 4.0 x 10(6), and 2.2 x 1 ... | 1978 | 749344 |
| isolation of bovine adenovirus type 1 without an adenovirus-associated virus. | this report describes the isolation and identification of a strain of bovine adenovirus type 1 from a cow in a herd that had undergone an episode of diarrhea and abortions. this strain of bovine adenovirus type 1 did not agglutinate either guinea pig or human 0 erythrocytes, and no 20-nm virus particles were evident by electron microscopy, further indicating that it was not contaminated by adenovirus-associated virus. | 1978 | 749583 |
| structural analysis of viral replicative intermediates isolated from adenovirus type 2-infected hela cell nuclei. | deoxyribonucleoprotein complexes released 17 h postinfection from adenovirus type 1 (ad2)-infected hela cell nuclei were shown by electron microscopy to contain filaments much thicker (about 200 a [20 nm]) than double-stranded dna (about 20 a [2 nm]). the complexes were partially purified through a linear sucrose gradient, concentrated, and further purified in a metrizamide gradient. the major protein present in the complexes was identified as the 72,000-dalton (72k), adenovirus-coded single-str ... | 1978 | 207893 |
| [enzymatic activity of the lymphocytes in gnotobionts in adenovirus infection]. | the cytochemical activity of succinate dehydrogenases, alpha-glycerophosphate (mitochondrial and hyaloplasmic), lactate, glycose-6-phosphate, glutamate, beta-oxibutyrate, nadp-h2- and nad-h2 diaphorases and acid phosphatase was studied in lymphocytes of gnotobiotic guinea-pigs infected with human adenovirus type 1. the analysis of transgression of distribution of the values in the experimental and control groups revealed a high informative diagnostic value of acid phosphatase and lactate dehydro ... | 1978 | 208307 |
| cross-protective immunity to canine adenovirus type 2 by canine adenovirus type 1 vaccination. | 1978 | 216291 | |
| [isolation of adenovirus type 1 from aborted cattle fetuses]. | five adenoviral strains, typed as adenovirus type 1, were isolated from aborted cattle foetuses, taken from farms with abortions in cows. the viruses were isolated from kidneys, lungs and livers of the foetuses. they were isolated from the kidney most frequenty (three strains). intranuclear basophilic inclusions in the alveolar epithelium of the lungs and in the tubular epithelium of the kidneys have been established in the histological investigations of the internal organs of the foetuses. the ... | 1978 | 223279 |
| adenovirus infection and cytopathic alterations of human cervical epithelial cells in vitro. | the effect of adenovirus infection was studied in monolayer cultures of human ecto- and endocervical epithelial cells. in both types of cultures adenovirus type 1 resulted in a slowly progressing lytic infection with persistent production of infectious virus, viral antigens and of cytopathic alterations in a fraction of cells. the changes were similar in ecto- and endocervical epithelial cells. although many of the alterations detected may represent unspecific lesions, nuclear changes consisting ... | 1978 | 282757 |
| replication of ovine adenoviruses. | the replication of ovine adenoviruses shows intertypic and intratypic variations, e.g. the extracellular and intracellular virus yield of ort/111, a strain related to bovine adenovirus type 2, reached its peak 40 to 46 hr postinfection, in contrast to gy/14, a strain classified as ovine adenovirus type 1, which required 52 to 58 hr to reach the highest yield. the replication cycle was not appreciably influenced either by rolling of the tube cultures or the age (between 0 and 7 days) of the suppo ... | 1978 | 382754 |
| studies on the antigenic relationship between an isolate (127) from the egg drop syndrome 1976 and a fowl adenovirus. | birds infected with fowl adenovirus type 1 (fav-1) developed precipitating antibody to fav-1 but not to haemagglutinating virus 127 isolated from flocks with a depressed egg production syndrome. once the precipitating antibody to fav-1 was no longer detectable, the birds were then infected with 127 virus. they developed precipitating and haemagglutination-inhibiting antibody to 127 virus. they also developed precipitating antibody to the adenovirus group antigen. birds infected only with 127 vir ... | 1978 | 18770415 |
| hexagonal crystalline arrays of adenovirus type 1 hexons. | separated, highly purified and concentrated adenovirus type 1 soluble hexon capsomers were crystallized by dialysis against 0.5 m acetate buffer. the crystallization process was followed electron microscopically. in the early phase of the crystallization, groups of a few hexons began to appear, then the two-dimensional crystal lattices grew gradually to a size of 1-2 micron. simultaneously three-dimensional crystals of tetrahedral and prismatic shapes developed. the hexons in the two-dimensional ... | 1979 | 539469 |
| [trial of a hyperimmune serum against para-influenza 3, bovine adenovirus 1, infectious rhinotracheitis and mucosal disease]. | a four-valent serum was obtained by parallel hyperimmunization of cattle with live viral suspensions of parainfluenza-3, bovine adenovirus i, the virus of infectious rhinotracheitis and the mucous disease virus. the serum's neutralizing titres are between 1:1280 and 1:2560. the lasting exploitation of serum producing cattle indicates that no competition exists between the four antigens used in the process of hyperimmunization. experimental testing of the serum on calves and its application in pr ... | 1979 | 231341 |
| detection of antibody titers to bovine viruses in rabbit sera. | sera obtained from 30 new zealand white rabbits from four different colonies were evaluated for the presence of immunoglobulins to 10 bovine viruses. serum neutralizing antibody titers to bovine adenovirus type 1 and to the enteroviruses types 3 and 4 were detected. | 1980 | 6253745 |
| electron microscopic studies on the crystallization of adenovirus hexon capsomers. | highly purified soluble adenovirus type 1 hexons were crystallized by dialyzing against 0.5 m acetate buffer, ph 4.5, for 3 days. crystals of tetrahedral shape were formed after storage at 4 degrees within 1--10 days and ranged in size from 0.01 to 0.1 mm. in early phase of crystallization, diffusely dispersed hexons were observed by electron microscopy. later, continuous monolayers of two-dimensional hexon paracrystalline arrays developed. direct analysis of the electron micrographs and their o ... | 1980 | 7358508 |
| hind iii restriction site map of the human adenovirus type 1 dna. | the physical map of human adenovirus type 1 dna was constructed with the hind iii restriction enzyme. direct comparison of the dna fragments with those of adenovirus type 2 revealed that the genome of adenovirus type 1 is 200 to 300 base pairs longer. the difference are located outside the region of the inverted terminal repetition within three distinct loci. fragment hind iii-f of type 1 dna which is presumed to carry all the genes responsible for in vitro transformation can be separated withou ... | 1981 | 6263226 |
| canine viruses and multiple sclerosis. | serum and cerebrospinal fluid from multiple sclerosis (ms) patients and control subjects were tested and compared for presence and titer of neutralizing antibody against the most common canine viruses. canine viruses included canine distemper virus (cdv), canine adenovirus 1 (cav-1), canine parainfluenza virus (cpiv), canine herpesvirus (chv), canine coronavirus (ccv), and canine parvovirus (cpv). neutralizing titers against measles virus (mv) and human adenovirus 8 (ha8) were also tested. signi ... | 1981 | 6267515 |
| [cytochemical study of granulocyte enzymes in germ-free animals with adenovirus infections]. | the activity of some dehydrogenases and hydrolases was studied by cytochemical methods in the peripheral blood neutrophils of germ-free guinea pigs infected with adenoviruses. the gnotobiotic animals were obtained by hysterotomy in an operation isolation room after which they were transferred into manipulation isolation room and infected with human adenovirus type 1. a depression of enzymes of alpha-glycerophosphate shunt and nadp-h2-diaphorase in neutrophils two days after infection and activat ... | 1981 | 6267824 |
| characterization of two strains of fowl adenovirus type 1. | an adenovirus isolated from the trachea of a chicken with respiratory disease was compared with an isolate obtained from the trachea of a clinically normal chicken. the isolates caused similar respiratory disease experimentally but differed in plaque morphology and fluorescent-antibody reactions in infected chick embryos. | 1981 | 6279068 |
| efficacy of amikacin and ticarcillin as empiric therapy in febrile neutropenic children with cancer. | amikacin (600 mg/m2/day iv) and ticarcillin (12 gm/m2/day iv) were administered to 38 children with cancer, neutropenia (less than 1,000 pmn/mm3), and fever (greater than or equal to 38 degrees c) during 46 febrile episodes. sixty-one percent of the children were severely neutropenic (less than 200 pmn/mm3). febrile episodes consisted of 15 documented (e coli 5, k pneumoniae and p aeruginosa 1, p. aeruginosa 1, s marcescens 1, s aureus 4, s epidermidis 1, s viridans 1, adenovirus 1), 13 clinical ... | 1981 | 7300805 |
| sequence homology between avian and human adenoviruses. | studies of hybridization between fowl adenovirus type 1 (chicken embryo lethal orphan virus) dna and human adenovirus type 2 dna revealed two short but distinct regions which cross-hybridized under stringent conditions. one of the homologous regions was located between map positions 18.1 and 19.3 and did not correspond to any gene recognized so far. the second region mapped in the hexon gene between position 57 and 58. | 1982 | 6283148 |
| isolation of an adenovirus antigenically distinct from equine adenovirus type 1 from diarrheic foal feces. | adenovirus was isolated in equine fetal kidney cell cultures from the feces of 2 foals with diarrhea that also had large numbers (greater than 10(6)/g) of rotavirus particles in their feces. unlike equine adenovirus type 1 (eadv1), the fecal eadv did not hemagglutinate human o, rhesus macaque, or equine rbc. by serum neutralization, the fecal viruses were identical with each other, but showed no relationship to eadv1. antiserum prepared against the fecal viruses did not contain hemagglutination- ... | 1982 | 6176153 |
| [radioimmunological study of the antigenic determinants of human adenovirus 1 hexon]. | 1982 | 6181383 | |
| in vitro blastogenesis of equine lymphocytes by inactivated equine adenovirus type 1 antigen. | an inactivated equine adenovirus type 1 (eadv1) vaccine was administered to 4 horses. the horses had virus-neutralizing (vn) antibody titers before they were vaccinated, but developed higher vn antibody titers in response to vaccination. nonvaccinated control horses did not show increases in vn antibody during the study, indicating that any increase in antibody titer in vaccinated horses was a result of vaccination and not due to an eadv1 epizootic during the study. specific eadv1 in vitro lymph ... | 1982 | 7181191 |
| [isolation of adenoviruses from calves with bronchopneumonia]. | the virologic investigation of clinical and pathological material from calves affected with a respiratory disease at the age of 30 days to 10 months on 16 infected stock-breeding farms led to the isolation of two virus strains (d-117 and ch-216) on primary cell cultures of fetal calf kidney from the nasal discharge of two 8-month-old calves. by their cytopathic changes, physico-chemical properties and the presence of a group-specific antigen they were said to belong to the adenovirus family. the ... | 1983 | 6659346 |
| polypeptides and immunoreactivity of empty adenovirus type 1 particles. | two distinct cscl-stable empty particle populations were characterized in human adenovirus type 1 infected cells. different sensitivity to proteases and polypeptide composition indicate that empty capsids a and b are degradation products of two different assembly intermediates. both empty particle populations react with antihexon antibodies similarly to complete virions. thus the arrangement of exposed type-specific epitopes is suggested to be unchanged during virus assembly. experimental eviden ... | 1983 | 6675409 |
| adenovirus serotypes isolated from ocular infections in london. | during the period 1973-8 700 adenoviruses were isolated from the eyes of patients presenting at moorfields eye hospital. of these, 678 were serotyped by a neutralisation test. twenty-one different serotypes were identified. serotype 3, 7, and 10 accounted for 68% of the isolates, 4, 8, 15 (15/29), and 19 for 25%, and the other 14 serotypes for 7%. community outbreaks of ocular infections by adenovirus 3, 4, 7, 10, and 15 (15/29) were observed. outbreaks with adenovirus 3, 7, and 10 appeared to c ... | 1983 | 6295436 |
| the 'blue eye' phenomenon. | diffuse clouding of the cornea ('corneal oedema', 'blue eye') of sudden onset and usually transient duration and with accompanying anterior uveitis, may be attributable to natural infection with canine adenovirus type 1 (ca-1) or to vaccination with live modified virus. it is now recognised that kerato-uveitis is a manifestation of type iii hypersensitivity in which immune complex formation resulting from the release of virus, especially from infected corneal endothelial cells, brings about corn ... | 1983 | 6304988 |
| unambiguous typing of canine adenovirus isolates by deoxyribonucleic acid restriction-endonuclease analysis. | viral deoxyribonucleic acid extracted from a limited number of cells infected with canine adenovirus type 1 or type 2 was cleaved with several restriction endonucleases. agarose gel electrophoresis of the limit digests showed stable differences between the canine adenovirus type 1 and type 2 cleavage patterns. rapid and accurate typing of large numbers of clinical isolates may thus be done by deoxyribonucleic acid restriction-endonuclease analysis. | 1983 | 6321002 |
| characterization of two serotypes of adenovirus isolated from sheep in the central united states. | two serotypes of adenovirus were isolated from lambs assembled at a ram testing station. the isolates were identified as adenoviruses on the basis of their morphologic and physicochemical characteristics. one isolate (rts-42) was neutralized by antiserum to ovine adenovirus serotype 5, whereas the other strain (rts-151) was not neutralized by the antisera to ovine adenovirus serotypes 1 through 5 or bovine adenovirus 1 through 8. | 1984 | 6324621 |
| interspecific differences between the dna restriction profiles of canine adenoviruses. | identification of canine adenovirus-1 ( cav -1) and canine adenovirus-2 ( cav -2) strains was done by electrophoresis of restriction endonuclease-fragmented viral dna. results obtained with this sensitive and reproducible technique clearly show that cav -2 is not a variant of cav -1 but a distinct virus. | 1984 | 6327360 |
| equine adenovirus 1 isolated from cauda equina neuritis. | equine adenovirus 1 was recovered after four to six passages from two out of three cases of cauda equina neuritis (cen) using kidney monolayers. similar treatment of lumbo-sacral spinal cord from six normal horses did not yield adenovirus. all three cases of cen had antibodies to the neuritogenic myelin protein p2 while immunofluorescence demonstrated that autologous igg bound to the myelin of affected nerves. adenovirus was not detected in neural tissue by immunofluorescence. | 1984 | 6390592 |
| characterization of adenovirus hexon epitopes by monoclonal antibodies. | three-thousand hybridoma clones were produced using crystallized hexon of human adenovirus type 1 for immunization. elisa revealed 69 homospecific antibody producing clones. the monoclonals were reactive with both crystallized and non-crystallized purified soluble hexon preparations, as well as with the hexons derived from purified virions. hexon preparations of 10 different human adenovirus species (members of 4 different subgenera) were used for the characterization of 33 monoclonal antibodies ... | 1984 | 6085607 |
| the structural proteins of chick embryo lethal orphan virus (fowl adenovirus type 1). | chick embryo lethal orphan (celo) virus (fowl adenovirus type 1) contains at least 14 structural proteins with polypeptide molecular weights ranging from 100k to about 6k. a nomenclature of the celo virion polypeptides is presented and the molar proportion of each polypeptide has been estimated. the celo virus pentons were specifically released from the virion by dialysis against borate-based calcium-magnesium saline. the penton base (polypeptide iii, mol. wt. 92k) and two fibres were separated, ... | 1984 | 6092524 |
| dna-binding proteins of chick embryo lethal orphan virus: lack of complementation between early proteins of avian and human adenoviruses. | chick cells infected by chick embryo lethal orphan (celo) virus (fowl adenovirus type 1) contained four prominent virus-specific, structurally related dna-binding proteins with mol. wt. of 74k, 64k, 56k, 52k, and two minor forms. the celo virus dna-binding proteins were phosphorylated, delayed-early nuclear proteins. celo virus early proteins were expressed in bhk cells, but did not complement human adenovirus type 5 mutants with lesions e1a, e2a or e2b. moreover, celo virus dna-binding proteins ... | 1984 | 6092525 |
| structural organization and polypeptide composition of the avian adenovirus core. | celo virus (fowl adenovirus 1) contained three core polypeptides of molecular weights 20,000, 12,000, and 9,500. the core was similar to that of human adenoviruses, with some evidence of compact subcore domains. micrococcal nuclease digestion of celo virus cores produced a smear of dna fragments of gradually decreasing size, with no nucleosome subunit or repeat pattern. moreover, when digested cores were analyzed without protease treatment, there was again no evidence of a nucleosome substructur ... | 1984 | 6092686 |
| infection rates of respiratory syncytial virus in pediatric patients attending phra mongkutklao hospital, bangkok. | respiratory syncytial virus (rsv) and other pathogens were isolated from nasopharyngeal secretions from 200 pediatric patients attending the out patient department of phra mongkutklao hospital with symptoms of upper respiratory tract infections. their sera were also taken for determination of class specific immunoglobulin antibody titers. the positive isolation rates were 36% for rsv, 5.5% for adenovirus 1.5% for herpes simplex virus (hsv), and 4% for staphylococcus aureus. one to 5.5% of these ... | 1984 | 6740381 |
| should the criteria for species distinction in adenoviruses be reconsidered? evidence from canine adenoviruses 1 and 2. | four strains of canine adenovirus type 1 (cav-1) and two strains of canine adenovirus type 2 (cav-2) were examined by restriction enzyme analysis. in all cases, cav-1 could be readily differentiated from cav-2. additionally, monoclonal antibodies were prepared against the mirandola and manhattan strains of cav-1 and cav-2, respectively. 18 of 36 monoclonal antibodies from the cav-1 fusion and 77 of 160 monoclonal antibodies from the cav-2 fusion were type-specific by an indirect fluorescent-anti ... | 1985 | 3884549 |
| lymphocyte function in recurrent aphthous ulceration. | lymphocyte blast transformation responses to mitogens and to streptococcal, adenovirus type 1, and oral mucosal antigens were studied in 32 patients with recurrent aphthous ulceration (rau) and matched control subjects. rau patients showed significantly depressed (p less than 0.05) responses to mitogens in comparison with matched controls, but there was no significant difference between patients and controls in response to any of the antigens tested. these results indicate that neither cell-medi ... | 1985 | 3930669 |
| isolation and characterization of six new genome types of human adenovirus types 1 and 2. | several of the 41 types of human adenovirus have been divided into genome types based on aberrant restriction endonuclease digestion patterns of viral dna. in the process of screening a large number of clinical adenovirus isolates by restriction endonuclease digestion of viral dna, we have identified nine isolates from eight patients which were identified by neutralization as adenovirus type 1 or 2 but had aberrant cleavage patterns. cleavage sites for the enzymes smai, ecori, hindiii, kpni, and ... | 1985 | 2981899 |
| a persistent adenovirus type 1 infection in synovial tissue from an immunodeficient patient with chronic, rheumatoid-like polyarthritis. | 1985 | 2985091 | |
| two distinctive transforming dna regions on the canine adenovirus type 1 genome. | by transfecting cloned dna fragments of canine adenovirus type 1, a novel transforming region of mammalian adenovirus was demonstrated inside the viral genome. besides the left-end smai-d fragment (left 17.2%), an internally located smai-e fragment (17.2-28.7%) of canine adenovirus type 1 was found to transform primary rat kidney cells. | 1985 | 2991059 |
| delineation of antigenic determinants of adenovirus hexons by means of monoclonal antibodies. | 60 hexon-specific mouse hybridoma clones were selected by using crystallized hexon capsomers of human adenovirus type 1 as immunizing and selecting antigen. the reactivities of the monoclonal antibodies were tested with purified hexon preparations from 10 human adenovirus types by enzyme-linked immunosorbent assay and passive hemagglutination techniques. the reactivity patterns delineated a total of 41 adenovirus-type-1-related determinants present on a number of heterologous hexons in different ... | 1985 | 2409054 |
| reactivity of mouse ascitic fluids containing monoclonal antibodies directed against adenovirus hexon. | thirty-two mouse hybridoma ascites were produced, containing hexon specific monoclonal antibodies, using crystallized hexons of human adenovirus type 1 as immunizing and selecting antigen. the reactivity of the ascitic fluids was tested with hexon preparations of 11 different human adenovirus species (serological types) belonging to 4 different subgenera by elisa and passive haemagglutination (ha) methods. by these methods the 32 hybridoma antibodies showed 4 and 12 distinct reactivity patterns ... | 1985 | 2412388 |
| differentiation of adenovirus hexon epitopes with monoclonal antibodies by gel diffusion assays. | nine monoclonal antibodies (mabs) directed against crystallized human adenovirus type 1 (ad h 1) hexon were tested with purified homologous and heterologous hexon preparations by gel diffusion. six mabs formed a single line with the homologous hexon in a 2-well pattern, and 3 mabs formed lines only in biclonal combinations with an appropriate mab. all of the 6 precipitating mabs formed a continuous line of complete identity when tested simultaneously against homologous and different heterologous ... | 1985 | 2413350 |
| grouping of monoclonal antibodies to adenovirus hexons by their cross-reactivity. | thirty two mouse ascitic fluids containing monoclonal antibodies (mabs) directed against the hexon of human adenovirus type 1 were examined. the type and degree of cross-reactivity (cr) of the mabs were determined by elisa and hemagglutination methods with 10 heterologous hexon types. the similarity and the dissimilarity of the mabs was also characterized by the correlation coefficient calculated from their reactivity values. on the basis of these, the 32 mabs could be divided into five groups a ... | 1986 | 2417575 |
| [stability of the structure and antigenic determinants of adenovirus type 1 native hexon to proteases]. | hexon capsomers of human adenovirus type 1 (h1) labeled by iodine 125 were digested in a native state (trimers) by trypsin, chymotrypsin or papain, and the resulting hydrolysates were analyzed by sds-page. in each case, a discrete and temporally stable pattern of relatively large fragments was revealed. the degree of hexon polypeptide hydrolysis was maximal for papain, intermediate for chymotrypsin and minimal for trypsin, the largest fragments in the digest being 32, 40 and 80 kd, respectively. ... | 1986 | 2429709 |
| multiple copies of identical epitopes on the adenovirus hexon. | a double monoclonal antibody (mab) sandwich enzyme-linked immunosorbent assay (double mab elisa), which uses the same mab as solid-phase immunosorbent (capture mab) and as detector mab (peroxidase-labeled), was developed to quantify the specific epitopes of adenovirus hexon. four mabs directed against crystallized adenovirus type 1 (ad h 1) hexon were tested by this assay with homologous and different heterologous hexons. the lowest reacting concn with the homologous and heterologous hexon types ... | 1986 | 2432411 |
| [features of the interaction of human adenovirus type 1 process with erythrocytes]. | 1986 | 2482434 | |
| evaluation of mixed cell types and 5-iodo-2'-deoxyuridine treatment upon plaque assay titers of human enteric viruses. | four continuous cell lines, bgm, l-132, hel-299, and rd, were compared both when cultured separately and as mixtures for use in plaque assay titrations of human adenovirus 1 and six human enterovirus serotypes. the effect of incubating these cell cultures in media containing 5-iodo-2'deoxyuridine (idu) prior to inoculation with virus was also studied. the use of mixed-cell cultures revealed cell line-dependent synergistic effects as well as inhibitory effects. these effects were strongly virus d ... | 1986 | 3015021 |
| changes of adenovirus hexon associated with different passage history of ad h 1. | two descendants of the prototype strain ad71-washington d. c. were obtained by independent passaging for at least 18 years in kiev, and in budapest (ad h 1 kappa, and ad h 1b, respectively). by restriction endonuclease mapping, the dna was identical corresponding to the patterns of human adenovirus type 1. in spite of this, sds-polyacrylamide gel electrophoresis revealed that the purified hexon of ad h 1 kappa was of lower mr than the subunit of ad h 1b. in contrast to this, the native capsomer ... | 1986 | 3105225 |
| comparative analysis of the canav-1 and canav-2 genomes. | the molecular weights of canine adenovirus type 1 (canav-1, strain utrecht) and type 2 (canav-2, strain toronto a26/61) dnas were determined by contour length measurements and restriction endonuclease analysis. in each case, an average molecular weight value of 20 (+/- 0.5) x 10(6) daltons was obtained with both methods. similar in size, the canav-1 and canav-2 dna molecules were, however, cleaved at very distinct positions by a variety of restriction endonucleases. the observed lack of dna sequ ... | 1986 | 3032842 |
| group c adenovirus dna sequences in human lymphoid cells. | human peripheral blood lymphocytes from healthy adults, cord blood lymphocytes, and lymphoblastoid cell lines were screened by hybridization for the presence of group c adenovirus dna sequences. in 13 of 17 peripheral blood lymphocyte samples from adults, 1 of 10 cord blood samples, and seven of seven lymphoblastoid cell lines tested, results were positive for group c adenovirus dna (adenovirus 1 [ad1], ad2, ad5, or ad6). about 1 to 2% of the lymphocytes carried 50 to 100 viral genome copies per ... | 1986 | 3486983 |
| [mutagenic effect of human adenovirus type i on the somatic and sex cells of male mice]. | human adenovirus 1 was studied for its effect on the chromosomal apparatus both in bone marrow cells and male sex cells of mice. chromosome aberrations were most early detected in spermatocytes of the 1st order mice infected with human adenovirus 1. in bone marrow cells of mice the highest level of chromosome aberrations was observed 30, 60, 90 days after the inoculation, which corresponds to a more frequent detection of the adenoviral antigen. the uv-irradiated-virus caused chromosome aberratio ... | 1986 | 3705168 |
| [antiviral action of lysolecithin analogs against human pathogenic viruses]. | the virucidal effect, the inhibition of virus adsorption and penetration and the influence on later phases of replication of human pathogenic viruses were studied. the compounds showed a significant virucidal effect to enveloped viruses (measles virus, herpes virus type 1 and 2), whereas the compounds were ineffective against nonenveloped viruses (coxsackie virus a9 and b1, attenuated poliovirus type 1, adenovirus type 1). interactions with the viral envelope are supposed. | 1986 | 3763682 |
| multiple reiteration of a 40-bp nucleotide sequence in the inverted terminal repeat of the genome of a canine adenovirus. | the dna of a vaccine strain of canine adenovirus type 1 [ichv vaccine; connaught laboratories, ltd.; cav-1(cll)] has been cloned in plasmid pat153 in the form of subgenomic bamhi digestion fragments. analysis of the nucleotide sequences of cloned terminal fragments has revealed an inverted terminal repeat (itr) with a minimum length of 198 nucleotides, including a tandem reiteration of the 40-bp nucleotide sequence from positions 14 to 53. the itrs had the 5'-catcatcaat ... sequence typical of a ... | 1987 | 3037784 |
| preferential clustering of viral dna sequences at or near the site of chromosomal rearrangement in fowl adenovirus type 1 dna-transformed cell lines. | all six transformants obtained by inoculating fowl adenovirus type 1 (celo virus) dna or its fragments into a rat cell line of normal karyotype had more than 50 copy-equivalents of viral dna sequences. each of the transformants had almost all if not all of these viral dna sequences clustered on a marker chromosome(s). although the marker chromosome(s) differed from one cell line to another, viral dna sequences preferentially clustered in or near the achromatic (or light-stained) region of the g- ... | 1987 | 3023693 |
| [immune response of calves following vaccination with paramyxovirus parainfluenzae-3 and adenovirus-1 under different colostral immunity stresses]. | studied was the immune response of calves, with colostrum antibodies and without colostrum antibodies, immunised with different dosages of live bivalent vaccine against p. parainfluenzae-3 and adenovirus-1. for the group of calves with colostrum immunity, after single hypodermic immunisation with doses from 10(4) till 10(5) cpe50/cm3 for adenovirus, was not proved immune response, as a result of blocking of the vaccinal antigen of colostrum antibodies. for calves which missed these antibodies, t ... | 1987 | 2833005 |
| distribution pattern of adenovirus hexon epitopes in infected cells determined with monoclonal antibodies by immunofluorescence analysis. | two monoclonal antibodies (mabs) specific for two distinct epitopes on the human adenovirus type 1 (av1) hexon were used to determine the subcellular localization of hexon epitopes in the infected hep-2 cells by indirect immunofluorescence. on the basis of cross-reactivity pattern of mabs, presumably one of the epitopes is genus specific and the other should be intertype specific. the epitopes, i.e. the adenovirus hexons could be detected throughout the cell and could display different accumulat ... | 1987 | 2452546 |
| antigenic structure of mammalian adenoviruses studied by monoclonal antibodies against bovine adenovirus type 2. | the cross-reactivity pattern of eight monoclonal antibodies (mabs) raised against bovine adenovirus type 2 (bav2) hexon was studied by indirect elisa using seven different adenoviruses. all of the eight mabs reacted with the two bav2 subtypes (a and b), while the other adenoviruses, human adenovirus type 1 (hav1), ovine adenovirus type 3 (oav3), bav1, bav4 and bav8 gave different combinations of positive reactions. based on the results of cross-reactivity there is a close antigenic relationship ... | 1988 | 2469284 |
| characterization of canine adenovirus type 1 isolated from american black bears. | viruses recovered from tissues taken at necropsy from american black bears were examined by use of immunofluorescence with polyclonal and monoclonal antibodies, virus neutralization with monoclonal antibodies, and restriction endonuclease analyses of the viral genomes. with these techniques, viruses were determined to be canine adenovirus type 1. seronegative dogs that were inoculated with the virus had clinical signs typical of infectious canine hepatitis, suggesting that the virus, which was v ... | 1988 | 2840839 |
| characterization of the genome of a vaccine strain of canine adenovirus type 1. | restriction endonuclease cleavage maps have been constructed for the genome of a canine adenovirus type 1 (cav-1) vaccine strain (cll; connaught laboratories, ltd., willowdale, ontario). restriction enzyme analyses were also carried out on cav-1 (cll) genomes isolated from viral stocks over 8 serial passages in a dog kidney cell line (dk 6722). the right hand 20% of the genome became more heterogeneous in size with increasing passage in dk 6722 cells due to deletions up to 3-4 kb, whereas the le ... | 1988 | 2852417 |
| identification of mouse adenovirus type 1 early region 1: dna sequence and a conserved transactivating function. | the left end of the genome of mouse adenovirus type 1 (also known as strain fl) was characterized by determination of the dna sequence, amino acid similarities with early region proteins of primate adenoviruses, and a functional assay. several specific dna sequence features were similar to those found in human adenoviruses, and open reading frames from this region could encode proteins similar to human adenovirus early region 1a and early region 1b proteins. dnas from this region were tested in ... | 1988 | 3172335 |
| serum antibody response to canine parvovirus, canine adenovirus-1, and canine distemper virus in dogs with known status of immunization: study of dogs in sweden. | serum antibody titers to canine parvovirus (cpv), canine adenovirus-1 (cav-1), and canine distemper virus (cdv) were measured in dogs with known immunization status. the dogs represented 3 groups: nonvaccinated dogs less than 12 months old; vaccinated dogs less than 12 months old; and adult dogs greater than 12 months old. for practical reasons, the population from which the specimens were obtained could be considered as free from natural infection with cav-1 and cdv. in nonvaccinated dogs less ... | 1988 | 3223653 |
| monoclonal antibodies to canine adenoviruses 1 and 2 that are type-specific by virus neutralization and immunofluorescence. | monoclonal antibodies were produced against the mirandola strain of canine adenovirus type 1 (cav-1) and the manhattan strain of canine adenovirus type 2 (cav-2). the monoclonal antibodies were used in vitro in virus neutralization (vn) assays and in indirect fluorescent antibody (ifa) tests to examine several strains of each virus type. out of 36 monoclonal antibodies produced against the mirandola strain, 18 were type-specific for cav-1 by ifa and 13 of those neutralized the virus in vitro. th ... | 1988 | 3281371 |
| the mouse adenovirus type 1 contains an unusual e3 region. | since the e3 region of human adenoviruses codes for a series of proteins that are probably involved in viral pathogenesis, the nucleotide sequence for a 3.6-kilobase dna fragment in the corresponding region (map units 77 through 89) of the mouse adenovirus type 1 genome has been determined. analysis of the sequence revealed that the genes for the fiber and for the precursor to the hexon-associated protein, pviii, that usually flank the e3 region, are well conserved. however, many of the open rea ... | 1989 | 2531236 |
| effects of vaccines on the canine immune system. | the effects of several commercially available polyvalent canine vaccines on the immune system of the dog were examined. the results demonstrated that the polyvalent vaccines used in this study significantly suppressed the absolute lymphocyte count and that most of the polyvalent vaccines significantly suppressed lymphocyte response to mitogen, but had no effect on natural effector cell activity, neutrophil chemiluminescence, nor antibody response to canine distemper virus. the individual vaccine ... | 1989 | 2540897 |