Publications

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stereodivergent construction of cyclic ethers by a regioselective and enantiospecific rhodium-catalyzed allylic etherification: total synthesis of gaur acid. 015366088
role of petiole in protein metabolism of senescing betel (piper betle l.) leaves.effects of depetiolation on protein metabolism during senescence of detached betel (piper betle l.) leaves have been studied. in normal petiolated leaves, the level of chlorophyll and proteins and extent of protein synthesis declined, while the protease activity registered manifold increase with the advancement of senescence. all of these changes were delayed by depetiolation/de-midribbing treatments, though without affecting the general pattern of senescence. thus, the presence of petiole seems ...197716659976
variation in height in relation to height vertex in gaur brahmins and chaudhury jats of meerut, uttar pradesh. 1978748183
endemic malignant catarrhal fever at the san diego wild animal park.malignant catarrhal fever was diagnosed in an indian gaur (bos gaurus gaurus), a barasingha deer, (cervus duvauceli duvauceli), and four javan banteng (bos javanicus javanicus) at the san diego wild animal park between july, 1976 and january, 1979. three of the four banteng lived adjacent to an exhibit in which wildebeast were born at 29.68 and 82 days prior to the banteng's deaths. the disease was characterized by pyrexia, conjunctivitis, diarrhea, dyspnea and rhinitis. mortality was 100%. post ...19807411750
comparative pathology of the african form of malignant catarrhal fever in captive indian gaur and domestic cattle.the african strain of malignant catarrhal fever virus was isolated and identified in a herd of captive indian gaur (bos gaurus). gross lesions included oral ulcerations, corneal opacity, and multifocal myocarditis. microscopic lesions were characterized by lymphocytic vasculitis/perivasculitis in all tissues examined. blood was serially passed from a gaur to a domestic heifer (bos taurus), and then to a domestic calf. clinical signs and gross and microscopic lesions similar to those of the gaur ...19817327993
sarcosporidiosis in indian gaur. 19816799471
a study of head growth among gaur brahmin boys. 19817286776
electron microscopic study of the african strain of malignant catarrhal fever virus in bovine cell cultures.malignant catarrhal fever (african strain) is a viral disease of ruminants which is considered an exotic disease in the united states. viral isolates obtained from one clinically ill gaur (bos gaurus) and a greater kudu (tragelaphus strepsiceros) located in a zoologic park in oklahoma, and from one heifer (bos taurus) and a domestic white-tailed deer (odocoileus virginianus) experimentally inoculated with the isolated and identified african strain of malignant catarrhal fever virus (mcfv), were ...19827073076
malignant catarrhal fever in an indian gaur and greater kudu: experimental transmission, isolation, and identification of a herpesvirus.herpesviruses were isolated in bovine cell cultures from buffy coat cells obtained from an indian gaur (bos gaurus) and a greater kudu (tragelaphus strepsiceros) with clinical signs of the head and eye form of malignant catarrhal fever (mcf). both animals were from herds housed in a zoologic park in oklahoma. serial transmission of the head and eye form of mcf was accomplished by using whole blood from the gaur into a hereford-angus heifer, then whole blood from the heifer into a holstein calf, ...19827091816
exertional rhabdomyolysis in an indian gaur. 19827174483
intra- and interspecific embryo transfer.the procedures that are collectively referred to as embryo transfer (et) have many uses. they were first used as research tools to study fetal-maternal physiology. since the first successful mammalian embryo transfer in 1890, et has been utilized for enhancement of genetic selection; diagnosis and treatment of infertility; control of infectious disease transmission; screening for genetic defects; propagation of rare and endangered species; and the study of developmental biology. most of the embr ...19836363609
karyotyping, red blood cell and haemoglobin typing of the mithun (bos frontalis), its wild ancestor and its hybrids.in bhutan in remote parts of the himalayas the mithun (bos frontalis) has been used probably for centuries in an ingenious system for crossbreeding with domestic cattle which results in highly profitable hybrid females. the hybrid males are infertile and so far no stable crossbreed has been developed. genetic analyses reported in this paper support the view that the gaur is the wild ancestor of the mithun. both have only 58 chromosomes in contrast to 60 in cattle and also different, hitherto und ...19846463373
amino-acid sequence of gayal hemoglobin (bos gaurus frontalis, bovidae).the amino-acid sequences of the alpha- and beta-chains of gayal hemoglobin have been determined and compared with those of bovine and yak hemoglobins. the gayal alpha-chain differs from the alpha-chains of bovine by 3 amino-acid residues and from yak i alpha- and ii alpha-hemoglobins by 4 and 2 residues, respectively. the gayal beta-chain differs from bovine beta a- and beta b-chains by 3 and 4 residues, respectively and from yak beta-chains by 2 residues.19846479895
levels of conservation and variation of heterochromatin and nucleolus organizers in the bovidae.chromomycin a3 banding of the mitotic sets of 10 species of bovidae (cattle, wisent, yak, banteng, gaur, red buffalo, swamp buffalo, sheep, mufflon, and goat) serves to demarcate both centromeric constitutive heterochromatin and r-banding patterns capable of identifying all the chromosomes within a given complement. in all species significant amounts of chromomycin-bright heterochromatin are present at the centromeres of all autosomes, though there was a high degree of intra- and inter-individua ...19854092166
chromosomes of gaur cross domestic cattle hybrids.the chromosomes of five gaur (bos gaurus hubbacki) domestic cattle (b indicus cross b taurus) hybrids (three females, two males) were studied using the leucocyte culture method and centromeric (c) banding technique. all the hybrids had a diploid chromosome number of 2n = 58, made up of two submetacentric autosomes (different in size) and 54 acrocentric autosomes, most of which could be arranged in pairs in descending order of size. the sex (x) chromosomes in females were a pair of submetacentric ...19883387680
effect of interferons on the replication of alcelaphine herpesvirus-1 of malignant catarrhal fever.four isolates of alcelaphine herpesvirus-1 of malignant catarrhal fever (mcf) were tested for their inducibility of and sensitivity to various interferons. viral isolates from an indian gaur (bos gaurus), a greater kudu (tragelaphus strepsiceros) and two wildebeest (connochaetes gnou) calves did not induce measurable interferon (ifn) in bovine fetal kidney cells. however, these low passages of each virus were all highly cell-associated and viral replication was inhibited at these passages by ifn ...19882457716
histology of a heifer placentome after interspecies transfer of a gaur embryo.histological examination of a single placentome recovered from a holstein heifer (bos taurus ) after delivery of a dead 9.5-mo-old gaur (bos gaurus ) calf revealed failure of proper development of the chorioallantoic villi after interspecies embryo transfer. macroscopically, the placentome appeared normal in size, its surface was rough, and on crosssection, the penetration of the villi was irregular and very different from the homogeneous penetration in developed cow placentomes. microscopically ...198816726502
cloning and characterization of the regulatory bacillus subtilis competence genes coma and comb.coma and comb are bacillus subtilis competence genes that are identified by insertions of tn917lac. they are classified as early genes because of their expression throughout growth; the expression of late com genes increases sharply during the transition to the stationary phase. the coma and comb determinants were cloned, and the 5' and 3' termini of their transcripts were localized by low-resolution s1 nuclease protection experiments. coma and comb were found by southern blotting to be localize ...19892507522
idiopathic epilepsy in three gaur (bos gaurus).idiopathic epilepsy was diagnosed in 3 gaur in a zoologic park collection during a 3-year period. seizures were characterized by tonic rigidity of all limbs. results of serum biochemical analysis, cbc, blood lead analysis, csf analysis, and necropsy were within normal limits in all gaur. the role of heredity in development of seizures in these gaur is unclear because of limited founder stock of gaur in north america.19892921210
nucleotide sequences of the bacillus subtilis flad locus and a b. licheniformis homologue affecting the autolysin level and flagellation.a dna fragment containing the flad locus of bacillus subtilis, which had been cloned into plasmid pac3, was subcloned into an m13 phage and sequenced. the sequence contained five open reading frames (orfs), of which orf2 was the flad gene. unexpectedly, the sequence of the flad locus was identical to that of sin [sporulation inhibition gene; gaur, n. k., dubnau, e. & smith, i. (1986). journal of bacteriology 168, 860-869]. a b. licheniformis homologue (flal) of the b. subtilis flad locus was clo ...19902121898
experimental observations on the pathogenesis of necrobacillosis.earlier studies showed that the minimum infective dose (greater than 10(6) organisms) of a virulent strain of fusobacterium necrophorum could be greatly reduced by suspending the fusobacteria in sub-lethal doses of cultures of other bacteria such as escherichia coli before inoculating mice subcutaneously. in the present study the infective dose of the same strain of f. necrophorum was reduced by a factor of greater than 10(3) by suspending the fusobacteria in sub-lethal doses of 5% homogenate of ...19902307186
foot and mouth disease in gayals (bos gaurus frontalis) in calcutta zoo.a brief description is given of an outbreak of foot and mouth disease in gayals (bos gaurus frontalis) which occurred in calcutta zoo in october 1990. four of the five affected animals died within ten days of onset of the disease. the disease did not spread to other animals in the zoo.19921472725
somatic cell mapping of conglutinin (cgn1) to cattle syntenic group u29 and fluorescence in situ localization to chromosome 28.a 260-bp genomic psti fragment, which encodes a portion of the carbohydrate recognition domain, was used along with hybrid somatic cells to map the conglutinin gene (cgn1) to domestic cow (bos taurus) syntenic group u29. in turn, a cosmid containing the entire bovine cgn1 was used with fluorescence in situ hybridization to sublocalize this gene to cattle chromosome (chr) (bta) 28 band 18. since bta 28 and several of the other small acrocentric autosomes of cattle are difficult to discriminate, w ...19938111119
malignant catarrhal fever in a gaur (bos gaurus) cow. 19938407480
the role of thymic epithelium in the establishment of transplantation tolerance.from experimental observations on induction of transplantation tolerance, we discuss a model that accounts for tissue-specific tolerance to antigens not expressed inside the thymus. it is postulated that antigens presented to differentiating t cells by thymic epithelium (or at large within the thymic environment) positively select and activate self-reactive t cells. a developmental program and/or prevalent conditions in the thymic environment restrict the proliferative potential and the class of ...19938225369
correction: omitted author.through an inadvertent error on my part, i neglected to include the name of one author-giovina ruberti-of the report "requirement for cd8(+) cells in t cell receptor peptide-induced clonal unresponsiveness" (1 jan., p. 91). the correct order of the authors is as follows: amitabh gaur, giovina ruberti, richard haspel, john p. mayer, and myself.199317838233
extraperitoneal laparoscopic pelvic lymph node dissection using the gaur balloon technique. 19948044516
[nephrectomy through lumbar endoscopy. report of 3 cases].after a long experience with transperitoneal laparoscopic surgery of the kidney, we have incorporated the gaur balloon technique for dissection. we report our experience of 3 nephrectomy procedures performed via lumboscopy, with the patients lying in the lumbotomy position. a 12 mm skin incision is made in the axillary midline. blunt dissection of the muscle planes is carried out and the retroperitoneal space is explored digitally. a 16 f balloon-tipped catheter is introduced and the balloon is ...19948053727
laparoscopic condom dissection: new technique of retroperitoneoscopy.the laparoscopic condom dissection is an advancement on the recently described balloon dissection technique of retroperitoneoscopy. it allows a simultaneous balloon dissection and retroperitoneal laparoscopic dissection using a telescope placed inside a condom balloon. in spite of a mild masking effect by the balloon, the clarity of vision was satisfactory, and adequate dissection could be carried out in all five patients in whom the condom was placed deep to the fascia transversalis or gerota's ...19948061674
oocyte maturation, fertilization and embryo development in vitro and in vivo in the gaur (bos gaurus).a study was conducted to evaluate the potential of rescuing immature oocytes from the ovaries of an endangered wild bovid, the gaur (bos gaurus). recovered, immature gaur oocytes (n = 59) placed in culture were evaluated for: (1) nuclear maturation after 22 h of culture, (2) fertilization with either thawed homologous (gaur) or heterologous (bos taurus) spermatozoa 18 h after insemination and (3) embryo development. gaur oocytes (n = 6) evaluated by fixation and staining at 22 h had all matured ...19948182580
diagnosis of malignant catarrhal fever by polymerase chain reaction amplification of alcelaphine herpesvirus 1 sequence.we derived sequence information from cloned hindiii fragment "d" of alcelaphine herpesvirus 1 strain wc11, an agent of malignant catarrhal fever (mcf). based on this sequence, oligonucleotide primers were selected and synthesized for use in a polymerase chain reaction amplification assay. these primers were used to test samples of total nucleic acids isolated from multiple tissues taken from an indian gaur (bos guarus gaurus) at the san diego wild animal park in san diego, california (usa) which ...19947933281
synthesis and secretion of retinol-binding protein and transthyretin by cultured retinal pigment epithelium.recent studies indicate that the retinal pigment epithelium (rpe) may serve as an extrahepatic source of retinol-binding protein (rbp) and transthyretin (ttr) for the retina by virtue of the fact that this cell layer is the exclusive retinal location for mrna coding for these proteins [herbert, j., et al. (1991) invest. ophthalmol. vis. sci. 32, 302-309; cavallaro, t., et al. (1990) invest. ophthalmol. vis. sci. 31, 497-501], although the proteins themselves are present in a variety of retinal n ...19948110786
ureteral laparoscopic surgery.during the past years, laparoscopy has grown in popularity and become an alternative technique for the treatment of certain pathologies in urological practice. retroperitoneal laparoscopic procedures for ureteral surgery were first attempted by wickham in 1979: in this case a patient with a ureteral calculus underwent a successful ureterolithotomy; more recently, the introduction of the gaur balloon has permitted quick dilatation and easier access to the retroperitoneum. however, in comparison, ...19957646000
[retroperitoneoscopy. technique and experiences with the first 100 patients].retroperitoneoscopic procedures were already being performed in the late 1970s. the clinical breakthrough of retroperitoneoscopy, however, was initiated in 1992 by the balloon-dissecting technique of gaur, together with the experience gained with transperitoneal laparoscopic procedures in the upper retroperitoneum. we have modified the balloon-dissecting technique into a hydraulic video-optically controlled balloon dissection of the retroperitoneal space, and this procedure was recently further ...19968711823
[extraperitoneal laparoscopic adrenalectomy: the initial 9 cases].(purpose). to determine the efficacy of extraperitoneal laparoscopic adrenalectomy for patients with adrenal tumor, the clinical results of 9 patients treated with this procedure were analysed. (patient and method). between july 1994 and march 1995, we have performed extraperitoneal laparoscopic adrenalectomy using the modified gaur technique of balloon dilatation of the retroperitoneum in 4 men and 5 women with unilaterla small adrenal tumor who were 30 to 79 years old (mean age 56 years). preo ...19968691705
a genetic map of bovine chromosome 7 with an interspecific hybrid backcross panel.a genetic linkage map of bovine chromosome (chr) 7 was generated with a bos taurus x bos gaurus interspecific hybrid backcross panel. this study included six previously mapped microsatellites and five unmapped expressed genes that were identified by pcr-based restriction fragment length variants (rflvs). the gene order (from centromere to telomere) and the map distances (in centimorgans) are as follows: cen-bm2607-11.2-ldlr-3.6-amh,csf2-11. 2-bp41-19-bm6117-19-sparc-14.4-fgfa-15.5-bm18 53-11.2-r ...19979096106
construction of a bovine chromosome 19 linkage map with an interspecies hybrid backcross.interspecific hybrid backcross animals from a bos taurus x bos gaurus f1 female were used to construct a linkage map of bovine chromosome (chr) 19. this map includes eight previously unmapped type i anchor loci, chrnb1, cryb1, gh1, myl4, nf1, p4hb, thra1, tp53, and five microsatellite markers, hel10, bp20, map2c, eth3, bmc1013, from existing linkage maps. the linkage relationship was determined to be centromere-hel10-18.8cm-nf1-4.0cm-cryb1-11 .2cm-(bp20, chrnb1, tp53)-4.0cm-(map2c, gh1, myl4, th ...19979096107
development and initial characterization of a bos taurus x b. gaurus interspecific hybrid backcross panel.a panel of 40 bos taurus x b. gaurus (gaur) interspecific hybrid backcross off spring was constructed for use as a tool in refining and improving the resolution of the bovine gene map. the gaur (2n = 58) is characterized karyotypically by a 2; 28 robertsonian translocation with respect to the cattle karyotype (2n = 60). this translocation was used as a centromeric marker to directionally orient linkage maps of b. taurus (bta) chromosomes 2 and 28. the initial linkage analysis of this panel consi ...19979378912
[malignant catarrhal fever in zoo ruminants].an outbreak of malignant catarrhal fever (mcf) in indian gauer (bos gaurus gaurus) and javan banteng (bos javanicus javanicus) occurred in the year 1964 and killed almost all animals of the groups of these species kept at the munich zoo at that time. in the consecutive years at highly irregular intervals cases of the head-and-eye-form of mcf occurred sporadically at the zoo in european and american bison (bison bonasus, b. bison' bison, b. bison athabascae), elk (alces alces), red deer (cervus e ...19989810608
heterologous fertilization to characterize spermatozoa of the genus bos.advances in assisted reproductive techniques, specifically, development of protocols for production of in vitro matured, fertilized and cultured domestic bovine embryos, offer opportunities to apply these techniques to nondomestic bovidae in species preservation. domestic bovine oocytes were inseminated with nondomestic bovine spermatozoa. effects of heparin concentration, sperm concentration and their interaction on total and normal in vitro fertilization rates and on subsequent embryo developm ...199810734485
retroperitoneoscopy: experience with 200 cases.a retroperitoneal access is commonly used for open urological procedures. since the introduction of the balloon dissecting technique by gaur this anatomical route has also been used for laparoscopic surgery. we present our experience with retroperitoneoscopy in 200 cases.19989751332
fluorescence quenching and time-resolved fluorescence studies on momordica charantia (bitter gourd) seed lectin.chemical modification studies implicated tryptophan (trp) residues in the sugar binding activity of momordica charantia lectin (mcl) [mazumdar, t., gaur, n. & surolia, a. (1981) eur. j. biochem. 113, 463-470]. in the present study, the accessibility and environment of trp residues in mcl were investigated by intrinsic fluorescence quenching and time-resolved fluorescence. the emission lamda max of native mcl in the absence as well as in the presence of 0.1 m lactose was around 335 nm, which shif ...19989739456
temporal 67gallium uptake is increased in temporal arteritis.we evaluated temporal 67gallium (ga) uptake in temporal arteritis (ta) and the contribution of ga scans to the diagnosis of ta.199910501416
gaur power. 200011102818
ubiquitinated sperm mitochondria, selective proteolysis, and the regulation of mitochondrial inheritance in mammalian embryos.the strictly maternal inheritance of mitochondria and mitochondrial dna (mtdna) in mammals is a developmental paradox promoted by an unknown mechanism responsible for the destruction of the sperm mitochondria shortly after fertilization. we have recently reported that the sperm mitochondria are ubiquitinated inside the oocyte cytoplasm and later subjected to proteolysis during preimplantation development (p. sutovsky et al., nature 1999; 402:371-372). here, we provide further evidence for this p ...200010906068
targeted disruption of the nkx3.1 gene in mice results in morphogenetic defects of minor salivary glands: parallels to glandular duct morphogenesis in prostate.to investigate functions of the homeodomain-containing transcription factor nkx3.1 a null mutation was generated by targeted gene disruption introducing the bacterial lacz gene as reporter into the locus. in addition to defects in duct morphogenesis of the prostate and bulbourethral gland displaying progressive epithelial hyperplasia and reduced ductal branching (bhatia-gaur, r., donjacour, a.a., sciavolino, p.j., kim, m., desai, n., young, p., norton, c.r., gridley, t., cardiff, r.d., cunha, g. ...200010906459
cloning of an endangered species (bos gaurus) using interspecies nuclear transfer.approximately 100 species become extinct a day. despite increasing interest in using cloning to rescue endangered species, successful interspecies nuclear transfer has not been previously described, and only a few reports of in vitro embryo formation exist. here we show that interspecies nuclear transfer can be used to clone an endangered species with normal karyotypic and phenotypic development through implantation and the late stages of fetal growth. somatic cells from a gaur bull (bos gaurus) ...200016218862
noah's new ark. the imminent birth of a cloned gaur signals a new approach to preservation. 200111194417
endangered species. cloned gaur a short-lived success. 200111228123
compromised development of calves (bos gaurus) derived from in vitro-generated embryos and transferred interspecifically into domestic cattle (bos taurus).advanced reproductive technologies, incuding ivf and interspecies embryo transfer, are becoming increasingly important for the preservation of endangered species. previous attempts at interspecies transfers between bos gaurus and bos taurus have yielded compromised offspring. the goal of this investigation was to characterize the effects of interspecies transfer of ivf-derived embryos on subsequent neonatal outcome. to achieve this goal, fresh bos gaurus ivf-derived embryos were transferred into ...200111354705
satellite-tagged transcribing sequences in bubalus bubalis genome undergo programmed modulation in meiocytes: possible implications for transcriptional inactivation.we cloned and sequenced a 1378 bp bamhi satellite dna fraction from the water buffalo bubalus bubalis and have studied its expression in different tissues. the gc-rich sequences of the resultant contig pds5 crosshybridize only with bovid dna and are not conserved evolutionarily. typing of buffalo genomic dna using pds5 with several restriction enzymes revealed multilocus monomorphic bands. similar typing of cattle, buffalo, goat, sheep, and gaur genomic dna revealed variations in copy number and ...200111747610
phylogeny of bovine species based on aflp fingerprinting.the bovini species comprise both domestic and wild cattle species. published phylogenies of this tribe based on mitochondrial dna contain anomalies, while nuclear sequences show only low variation. we have used amplified fragment length polymorphism (aflp) fingerprinting in order to detect variation in loci distributed over the nuclear genome. computer-assisted scoring of electrophoretic fingerprinting patterns yielded 361 markers, which provided sufficient redundancy to suppress stochastic effe ...200211813106
[dna fingerprinting of individual species and intergeneric and interspecific hybrids of genera bos and bison, subfamily bovinae].genome fingerprinting with a hypervariable minisatellite sequence of phage m13 dna was used to study the genetic variation in individual species of the genera bos and bison (subfamily bovinae) and in their interspecific and intergeneric hybrids. dna fingerprints were obtained for domestic cow bos taurus primigenius, vatussy bos taurus macroceros, banteng bos javanicus, gaur bos gaurus, wisent bison bonasus, bison bison bison, and for the interspecific and intergeneric hybrids. compared with the ...200212018169
consequences of manipulating gametes and embryos of ruminant species.during the past 12 years, ruminants have provided a focus for some significant advances in mammalian reproductive biotechnologies. lambs were the first offspring generated after nuclear transfer of fetal or adult cells to enucleated oocytes, and many calves of pre-determined gender are today the result of commercialized semen sexing. in 1990, the birth of one calf provided living proof that even 'dead' spermatozoa can be paternal, whereas, more recently, a short-lived gaur calf and viable mouflo ...200314635934
inbreeding, mortality, and sex ratio in gaur (bos gaurus) under captivity.the average inbreeding coefficient for gaur (bos gaurus h. smith) born in captivity has ranged from 0.139 to 0.234 per year since 1969. juvenile mortality is significantly higher among inbred young, and a significantly higher proportion of males occur in inbred than in noninbred offspring.20037108189
eye care project in gaur, nepal.the aim of the eye care project is to clear the backlog of cataract blindness for the people of gaur and its surrounding districts in southeast nepal. the purpose of this study is to analyze the progress of this eye care project.200415460911
maternal and paternal lineages in cross-breeding bovine species. has wisent a hybrid origin?the tribe bovini comprises cattle and cattle-like species. reconstructions of their phylogeny have so far been incomplete and have yielded conflicting conclusions about the relationship of american bison and wisent (european bison). we have compared the sequences of three mitochondrial and two y-chromosomal dna segments. mitochondrial dna indicates that four distinct maternal lineages diverged after an early split-off of the buffalo species, leading to (1) taurine cattle and zebu, (2) wisent, (3 ...200414739241
epigenetic and genomic imprinting analysis in nuclear transfer derived bos gaurus/bos taurus hybrid fetuses.somatic cell nuclear transfer (nt) in cattle is an inefficient process, whereby the production of calves is hindered by low pregnancy rates as well as fetal and placental abnormalities. interspecies models have been previously used to facilitate the identification of single nucleotide polymorphisms (snps) within coding regions of genes to discriminate between parental alleles in the offspring. here we report the use of a bovine interspecies model (bos gaurus x bos taurus) for the assessment and ...200415044262
absolute configuration of a hydroxyfuranoid acid from the pelage of the genus bos, 18-(6s,9r,10r)-bovidic acid.pelage extracts of the banteng (bos javanicus), the domestic cattle (b. taurus), the gaur (b. frontalis), and the yak (b. grunniens) were investigated by fabms and nmr. an 18-carbon hydroxyfuranoid acid, 1 (10-hydroxy-6,9-oxidooctadecanoic acid), first reported from the wool of the domestic sheep (ovis aries) was confirmed in b. frontalis; we suspend judgment on the occurrence of this compound in the other species we examined. the stereochemistry of 1, determined by mosher nmr and cd tweezer met ...200415332870
identification of the homologue of the bovine rob(1;29) in a captive gaur (bos gaurus).robertsonian translocations have been well documented in domestic cattle, with the most commonly occurring fusion involving chromosomes 1 and 29. the widespread nature of this translocation is indicative of its ancient origin. the gaur (bos gaurus) is one of many wild cattle species currently listed as vulnerable or endangered. due to the small founder stock and 50 years of restricted breeding, the captive herd is showing signs of inbreeding and reduced fertility. recent cytogenetic analysis of ...200415505407
myostatin rapid sequence evolution in ruminants predates domestication.myostatin (gdf-8) is a negative regulator of skeletal muscle development. this gene has previously been implicated in the double muscling phenotype in mice and cattle. a systematic analysis of myostatin sequence evolution in ruminants was performed in a phylogenetic context. the myostatin coding sequence was determined from duiker (sylvicapra grimmia caffra), eland (taurotragus derbianus), gaur (bos gaurus), ibex (capra ibex), impala (aepyceros melampus rednilis), pronghorn (antilocapra american ...200415522803
molecular phylogeny of the tribe bovini (bovidae, bovinae) and the taxonomic status of the kouprey, bos sauveli urbain 1937.the kouprey is a very rare bovid species of the indochinese peninsula, and no living specimen has been described for a long time, suggesting that it is possibly extinct. its systematic position within the tribe bovini remains confused since the analyses of morphological characters have led to several conflicting hypotheses. some authors have also suggested that it could be a hybrid species produced by the crossing of the banteng with gaur, zebu, or water buffalo. here we performed a molecular ph ...200415522811
conservation of genomic imprinting at the xist, igf2, and gtl2 loci in the bovine.genomic imprinting is theorized to exist in all placental mammals and some marsupials; however, extensive comparative analysis of animals aside from humans and mice remains incomplete. here we report conservation of genomic imprinting in the bovine at the x chromosome inactivation-specific transcript (xist), insulin-like growth factor 2 (igf2), and gene trap locus 2 (gtl2) loci. coding single nucleotide polymorphisms (snps) between bos gaurus and bos taurus were detected at the xist, igf2, and g ...200415599555
close genetic relationship of early neolithic cattle from ziegelberg (freising, germany) with modern breeds.in 2003 a variety of crafts and bone specimens were found during excavations of a neolithic settlement near freising, the southernmost site of the linear pottery culture in bavaria. six cattle bones were used to extract ancient dna (adna). applying nested and touchdown pcr, two fragments of the mitochondrial (mt) dna control region could be amplified from specimen 533/iii which yielded a total of 230 base pairs (bp). the sequence was compared with the homologous part of 40 extant breeds of bos t ...200516130455
clostridial abomasitis in calves: case report and review of the literature.infections by clostridium perfringens type a are perhaps the most common causes of clostridial hemorrhagic enteritis in neonatal ruminants. affected calves exhibit tympany, hemorrhagic abomasitis, and abomasal ulceration. gram-positive bacilli are often found on affected mucosa and in submucosa. aspects of etiology beyond the infecting organism are little understood, but probably include dietary issues, perhaps relating to overfeeding, feeding of barely thawed or contaminated colostrum, or condi ...200516701586
ethnomedicinal uses of momordicacharantia (cucurbitaceae) in togo and relation to its phytochemistry and biological activity.investigation of the traditional uses of momordica charantia (cucurbitaceae) in togo (west africa) showed that it is one of the most important local medicinal plants both for ritual and ethnomedical practices. there was a high degree of consensus (>50%) for use in the treatment of gastrointestinal and viral disease among 47 groups of village informants in the general population, while 19 traditional healers reported a larger and broader set of uses. the use by informants in gaur and kwa language ...200515588650
guggul for hyperlipidemia: a review by the natural standard research collaboration.to evaluate the scientific evidence on guggul for hyperlipidemia including expert opinion, folkloric precedent, history, pharmacology, kinetics/dynamics, interactions, adverse effects, toxicology, and dosing.200516338199
has the kouprey (bos sauveli urbain, 1937) been domesticated in cambodia?the kouprey (bos sauveli urbain, 1937) is a very rare bovid species of cambodia, which may be extinct in the wild, as no living specimen has been observed for a long time. here, we describe a complete taxidermy mount, which presents astonishing morphological similarities with the kouprey. the animal was mounted in 1871 at the national museum of natural history in paris, where it was referenced as no. 1871-576. it was deposited at the natural history museum of bourges, france, in 1931, where it i ...200616439342
threonine-rich repeats increase fibronectin binding in the candida albicans adhesin als5p.commensal and pathogenic states of candida albicans depend on cell surface-expressed adhesins, including those of the als family. mature als proteins consist of a 300-residue n-terminal region predicted to have an immunoglobulin (ig)-like fold, a 104-residue conserved thr-rich region (t), a central domain of a variable number of tandem repeats (tr) of a 36-residue thr-rich sequence, and a heavily glycosylated c-terminal ser/thr-rich stalk region, also of variable length (n. k. gaur and s. a. klo ...200616936142
the influence of nuclear content on developmental competence of gaur x cattle hybrid in vitro fertilized and somatic cell nuclear transfer embryos.in nondomestic and endangered species, the use of domestic animal oocytes as recipients for exotic donor nuclei causes the normal pattern of cytoplasmic inheritance to be disrupted, resulting in the production of nuclear-cytoplasmic hybrids. evidence suggests that conflict between nuclear and cytoplasmic control elements leads to a disruption of normal cellular processes, including metabolic function and cell division. this study investigated the effects of nuclear-cytoplasmic interactions on th ...200717151347
phylogenetic relationships and status quo of colonies for gayal based on analysis of cytochrome b gene partial sequences.thirty-three mutations and four different haplotypes were found when cytochrome b(cytb) gene partial sequences of 12 gayals were analyzed. together with sequences of bos indicus, bos taurus, bos grunniens and bos gaurus with bubalus bubalis as the out group, the partial sequences of cytb gene of gayals were aligned and base composition and nucleotide variation of cytb gene were analyzed. the phylogenetic trees were constructed by the nj method and the mp method respectively, both supporting almo ...200717560527
resolving a zoological mystery: the kouprey is a real species.the kouprey is a rare and enigmatic forest ox discovered by scientists in cambodia only in 1937. numerous morphological hypotheses have been proposed for the origin of the kouprey: that it is a species closely related to banteng and gaur, two other wild oxen of southeast asia; a morphologically divergent species placed in a separate genus, named novibos; a wild species linked to aurochs and domestic cattle; a vicariant population of banteng; a feral cattle; or a hybrid of banteng with either zeb ...200717848372
genomic conservation of cattle microsatellite loci in wild gaur (bos gaurus) and current genetic status of this species in vietnam.the wild gaur (bos gaurus) is an endangered wild cattle species. in vietnam, the total number of wild gaurs is estimated at a maximum of 500 individuals. inbreeding and genetic drift are current relevant threats to this small population size. therefore, information about the genetic status of the vietnamese wild gaur population is essential to develop strategies for conservation and effective long-term management for this species. in the present study, we performed cross-species amplification of ...200717986322
new robertsonian translocation chromosomes in captive thai gaur (bos gaurus readei).robertsonian translocation have been well documented in domestic cattle, with the most commonly occurring fusion involving chromosomes 1 and 29. the widespread nature of this translocation is indicative of its ancient origin. fifty giemsa's stained metaphase spreads derived from lymphocyte cultures of the thai gaur were analyzed for each animal. the thai gaur had diploid chromosome number of 2n = 57 in male and 2n = 56 in female instead of the normal 2n = 58. the 2n = 57 in male chromosomes pres ...200719070179
species composition and community structure of dung beetles attracted to dung of gaur and elephant in the moist forests of south western ghats.the community structure of dung beetles attracted to dung of gaur, bos gaurus (h. smith) (artiodactyla: bovidae) and asian elephant, elephas maximus linnaeus (proboscidea: elephantidae), is reported from the moist forests of western ghats, in south india. the dominance of dwellers over rollers, presence of many endemic species, predominance of regional species and higher incidence of the old world roller, ochicanthon laetum, make the dung beetle community in the moist forests of the region unusu ...200720337551
statistical evaluation of influence of xanthan gum and guar gum blends on dipyridamole release from floating matrix tablets.the present investigation explored the use of xanthan gum and guar gum for development of floating drug delivery system of dipyridamole using factorial design approach. the content of polymer blends (x(1)) and ratio of xanthan gum to guar gum (x(2)) were selected as independent variables. the diffusion exponent (n), release rate constant (k), percentage drug release at 1 hr (q(1)) and 6 hr (q(6)) were selected as dependent variables. tablets of all batches had desired buoyancy characteristics. m ...200717454065
influence of zoo visitor presence on the behavior of captive indian gaur (bos gaurus gaurus) in a zoological park.visitors to zoos can be a source of potential disturbance and stress to some captive, nonhuman animals in the wild. to determine the influence of visitor presence on captive bison (bos gaurus gaurus), the study analyzed the behavior of 4 individuals at the arignar anna zoological park, india. the study often observed the behavior of the animals on visitor-present days and on days when visitors were absent. in the presence of zoo visitors, the bison showed a higher level of intragroup aggression ...200818821404
candida albicans als adhesins have conserved amyloid-forming sequences.the cell wall-bound als adhesins of candida albicans mediate both yeast-to-host tissue adherence and yeast aggregation. this aggregation is amyloid-like, with self-propagating secondary-structure changes, amyloid-characteristic dye binding, and induced birefringence (j. m. rauceo, n. k. gaur, k. g. lee, j. e. edwards, s. a. klotz, and p. n. lipke, infect. immun. 72:4948-4955, 2004). therefore, we determined whether als proteins could form amyloid fibers with properties like those in cellular agg ...200818083824
[molecular phylogeny of the gayal inferred from the analysis of cytochrome b gene entire sequences].the gayal (bos frontalis) is a very rare, semi-wild and semi-domestic bovine species. there still exist remarkable divergences on the gayal's origin and phylogenetic status. the cytochrome b (cyt b) gene entire sequences (1,140 bp) of 11 gayals were sequenced and analyzed. combined with other bovine cyt b entire sequences cited in genbank, the phylogenetic trees of genus bos were reconstructed by neighbor-joining (nj) and maximum parsimony (mp) methods with bubalus bubalis as outgroup. sequence ...200818244904
short communication: new alleles of the bovine kappa-casein gene revealed by resequencing and haplotype inference analysis.we tested the hypothesis that extensive undiscovered genetic diversity exists in important functional genes from domestic and wild cattle species (bos spp.). we resequenced 483 bp of a key exon (exon iv) from the kappa (kappa)-casein gene (csn3) for a panel of samples of domestic cattle from 8 countries and a close relative species, the gayal (bos frontalis). six single nucleotide polymorphisms were identified. haplotype inference revealed 12 haplotypes, of which 8 were newly discovered. among t ...200818765626
impact of human immunodeficiency virus type 1 resistance to protease inhibitors on evolution of resistance to the maturation inhibitor bevirimat (pa-457).the maturation inhibitor bevirimat [3-o-(3',3'dimethysuccinyl)betulinic acid; bvm; also known as pa-457 or dsb] potently inhibits human immunodeficiency virus type 1 (hiv-1) replication by blocking protease (pr)-mediated cleavage at the junction between capsid (ca) and spacer peptide 1 (sp1) in gag. we previously isolated a panel of single-amino-acid substitutions that confer resistance to bvm in vitro (c. s. adamson, s. d. ablan, i. boeras, r. goila-gaur, f. soheilian, k. nagashima, f. li, k. s ...200919279107
species identification, molecular sexing and genotyping using non-invasive approaches in two wild bovids species: bos gaurus and bos javanicus.since the second indochina war, habitat destruction and overhunting has resulted in fragmentation of the remaining populations of bos javanicus and b. gaurus. nowadays, both species are in serious danger, especially the gaur. in vietnam, where these species have become almost impossible to capture in the wild, non-invasive investigations are the only feasible approach to obtain data on populations. however, non-invasive derived dna, especially in tropical areas, is usually characterized by low c ...200919367625
phylogenetic reconstruction and the identification of ancient polymorphism in the bovini tribe (bovidae, bovinae).the bovinae subfamily incorporates an array of antelope, buffalo and cattle species. all of the members of this subfamily have diverged recently. not surprisingly, a number of phylogenetic studies from molecular and morphological data have resulted in ambiguous trees and relationships amongst species, especially for yak and bison species. a partial phylogenetic reconstruction of 13 extant members of the bovini tribe (bovidae, bovinae) from 15 complete or partially sequenced autosomal genes is pr ...200919393045
karyotype of malayan gaur (bos gaurus hubbacki), sahiwal-friesian cattle and gaur x cattle hybrid backcrosses.interspecific hybridization has been reported for a wide variety of vertebrate species either spontaneous or by organized crossing of bovine species. the hybrids were often carrying intermediate characters genetically and phenotypically of the parents. thus, status information of both aspects is valuable in animal production for selection and breeding management. the gaur-cattle hybrids was reported to be superior in production value compared to their parent cattle but fertility status was still ...200919777782
structure and function of glycosylated tandem repeats from candida albicans als adhesins.tandem repeat (tr) regions are common in yeast adhesins, but their structures are unknown, and their activities are poorly understood. tr regions in candida albicans als proteins are conserved glycosylated 36-residue sequences with cell-cell aggregation activity (j. m. rauceo, r. de armond, h. otoo, p. c. kahn, s. a. klotz, n. k. gaur, and p. n. lipke, eukaryot. cell 5:1664-1673, 2006). ab initio modeling with either rosetta or linus generated consistent structures of three-stranded antiparallel ...201019820118
diversity and phylogeny of mitochondrial dna isolated from mithun bos frontalis located in bhutan.we sequenced the 16s rrna gene in mitochondrial dna to characterize mithun located in bhutan and to increase our understanding of its origin. we compared mithun with yak, european cattle, bhutanese zebu and indian zebu. sequencing revealed low nucleotide diversity within the mithun population and their phylogenetic proximity to gaur. a close relationship between bhutanese mithun and gaur was confirmed by an additional comparison with wild gaur specimens from three locations in bhutan. direct dom ...201020331596
polymorphisms in the bovine hemoglobin-beta gene provide evidence for gene-flow between wild species of bos (bibos) and domestic cattle in southeast asia.the electrophoretic variation in bovine hemoglobin-beta (hbb) is one of the most investigated genetic markers. the presence of a unique hbb variant, hbb(x), in southeast asian cattle has been reputed as a sign of gene-flow from wild bovine species. in this study, we analyzed the dna sequences of hbb genes in domestic and wild bovine species to verify this belief. isoelectric focusing of hbb chain revealed that the hbb(x) in domestic cattle had dimorphism and was separated into hbb(x1) and hbb(x2 ...201021269357
molecular phylogeny and diversity of myanmar and bhutan mithun based on mtdna sequences.the mithun (bos frontalis), synonymous with mithan and gayal, is considered to be a domesticated form of gaur (b. gaurus). however, there has been a controversy concerning its origin. in an effort to address this issue, the mitochondrial cytochrome b (cytb) genes of 20 mithun from myanmar and 13 from bhutan were sequenced to trace its maternal origin. seven cytb haplotypes were found in the 33 mithun, and the phylogenetic tree for these haplotypes clearly showed three embranchments involving fiv ...201021269359
the prevalence of blindness and cataract surgery in rautahat district, nepal.the gaur eye hospital, which provides eye care services to the people in rautahat and adjacent districts, completed 9 years of operation in 2006. over 14,000 cataract surgeries were performed during this period. this study aimed to ascertain the impact of the hospital services by estimating the prevalence of blindness, visual impairment and cataract surgical coverage among the older adult population of the rautahat district.201020302430
evaluation of the role of preoperative double-j ureteral stenting in retroperitoneal laparoscopic pyelolithotomy.since the first retroperitoneal laparoscopic pyelolithotomy (rppl) was reported by gaur and associates in 1994, its technique has improved considerably. the applicability and indications of the procedure are expanding with advances in technology, expertise, and experience. to date, there has been no prospective study in the literature about the role of preoperative double-j (d-j) ureteral stenting in patients who undergo rppl. this study is an endeavor to evaluate the role of preoperative d-j st ...201020044764
studies on amendment of different biopolymers in sandy loam and their effect on germination, seedling growth of gossypium herbaceum l.different biopolymers, agar, cellulose, alginate, psyllium gaur gum, and bacterial exopolysaccharide (eps) powders were amended to check their efficacy in enhancing maximum water holding capacity (mwhc), permanent wilting point (pwp), and germination and seedling growth of the gossypium herbaceum in a laboratory scale. the efficacy of all biopolymers for enhancement of mwhc, pwp, and growth was also analyzed by measuring organic carbon, organic matter, total nitrogen, respiration rate, and micro ...201120859704
optimization of pcr conditions to amplify cyt b, coi and 12s rrna gene fragments of malayan gaur (bos gaurus hubbacki) mtdna.pcr has been extensively used for amplification of dna sequences. we conducted a study to obtain the best amplification conditions for cytochrome b (cyt b), cytochrome c oxidase i (coi) and 12s rrna (12s) gene fragments of malayan gaur mtdna. dna from seven malayan gaur samples were extracted for pcr amplification. various trials and combinations were tested to determine the best conditions of pcr mixture and profile to obtain the best pcr products for sequencing purposes. four selected target f ...201122033937
diet composition and quality in indian bison (bos gaurus) based on fecal analysis.diet composition and quality of the indian bison (bos gaurus) was estimated by fecal analysis. the results, together with studies in other parts of india, indicate that gaurs are primarily intermediate or adaptable mixed feeders. fecal composition varied seasonally, with high proportion of grasses, forbs, and woody plant leaves, particularly cynodon dactylon, cyperus rotundus in monsoon and post monsoon, and strobilanthes callosus, strobilanthes ixiocephalus, grewia tiliaefolia and syzygium cumi ...201121466343
phylogenetic relationships of malayan gaur with other species of the genus bos based on cytochrome b gene dna sequences.the malayan gaur (bos gaurus hubbacki) is one of the three subspecies of gaurs that can be found in malaysia. we examined the phylogenetic relationships of this subspecies with other species of the genus bos (b. javanicus, b. indicus, b. taurus, and b. grunniens). the sequence of a key gene, cytochrome b, was compared among 20 bos species and the bongo antelope, used as an outgroup. phylogenetic reconstruction was employed using neighbor joining and maximum parsimony in paup and bayesian inferen ...201121476194
Ethnobotany of the Monpa ethnic group at Arunachal Pradesh, India.The present paper documents the uses of plants in traditional herbal medicine for human and veterinary ailments, and those used for dietary supplements, religious purpose, local beverage, and plants used to poison fish and wild animals. Traditional botanical medicine is the primary mode of healthcare for most of the rural population in Arunachal Pradesh.201121995750
bladder neck preservation during classic laparoscopic radical prostatectomy - point of technique and preliminary results.stress urinary incontinence after radical prostatectomy for prostate cancer organ-confined patients can significantly affect quality of life. the article presents a technique of bladder neck preservation, because it is believed that this point is one of many crucial points responsible for fast recovery of continence after laparoscopic radical prostatectomy (lrp).201123256008
comparison of carfentanil-xylazine and thiafentanil-medetomidine in electroejaculation of captive gaur (bos gaurus).carfentanil citrate and thiafentanil oxalate have been used successfully to immobilize captive and free-ranging ungulates. the objective of this study was to compare the efficacy and certain physiologic parameters of protocols by using the 2 opioids in gaur (bos gaurus). eight adult gaur bulls were immobilized for electroejaculation at omaha's henry doorly zoo (omaha, nebraska, usa). all the animals were immobilized twice, by using each of the following protocols one time: 10 mg carfentanil comb ...201122950315
karyotype analysis of mithun (bos frontalis) and mithun bull x brahman cow hybrids.we examined the cytogenetics of mithun (bos frontalis), a domesticated version of the asian gaur, and hybrids (f(1) generation) produced by artificial insemination of brahman cows (bos indicus) with mithun semen. reproductive potential was also examined in the f(1) generation and a backcrossed heifer for utilization of heterosis. metaphase chromosome spreads were examined by conventional staining and fluorescence in situ hybridization hybridized with the entire chromosome 1 of mithun as a specif ...201222290473
cellulose-polymer-ag nanocomposite fibers for antibacterial fabrics/skin scaffolds.natural carbohydrates (polysaccharides): gum acacia (ga) and gaur gum (gg) were employed in dilute solutions: 0.3%, 0.5% and 0.7% (w/v), as effective reductants for the green synthesis of silver nanoparticles (agnps) from agno3. the formed agnps were impregnated into cellulose fibers after confirming their formation by utilizing ultraviolet-visible (uv-vis) spectral studies, fourier transforms infrared (ftir) and transmission electron microscopy (tem). the surface morphology of the developed cel ...201223499096
collection, analysis and cryopreservation of semen from malayan gaur (bos gaurus hubbacki): a preliminary study.the malayan gaur (bos gaurus hubbacki) or seladang is classified as vulnerable by the international union for conservation of nature and natural resources (iucn). the malayan gaur is mainly distributed in the tropical woodlands of peninsular malaysia and southern thailand. the aim of this study was to collect, analyze and cryopreserve the semen of wild malayan gaur. transrectal massage (tm) and electroejaculation (eej) technique was applied in semen collection of the malayan gaur. the semen was ...201226623302
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