Publications

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the cerebral hemispheres of the kiwi and of the emu (apteryx and dromiceius). 193517104544
some physicochemical properties of the three albumin layers of the chicken, goose and emu eggs. 194720247649
adjustable aperture for electron microscope, rca type emu. 194818871501
the ultrastructure of mouse lung: the alveolar macrophage.free alveolar macrophages of normal mouse lung have been studied in the electron microscope. the tissue was obtained from several young adult white mice. one other animal was instilled intranasally with diluted india ink 1(1/2) hours prior to the removal of the lung. thin sections of the osmium-fixed, methacrylate-embedded tissue were examined either in an rca emu 2 electron microscope or in a siemens and halske elmiskop i b. a few thick sections obtained from the same embeddings were stained fo ...195813610931
electron microscope observations on the behavior of the bacterial cytoplasmic membrane during cellular division.bacterial cells were fixed in oso(4), washed, dehydrated, and embedded in a methacrylate mixture. ultrathin sections were cut on a porter-blum ultramicrotome and were examined in an rca electron microscope, type emu-2d. the sections revealed that the cytoplasmic membrane undergoes a centripetal growth to form a membrane septum. this septum is formed as a double structure. constriction of the daughter cells and deposition of cell wall material lead to the separation of the daughter cells. the bac ...195913809225
spermiogenesis in lumbricus herculeus; an electron microscope study.small pieces of the sperm sacs of lumbricus herculeus were fixed for 4 hours in chrome-osmium, embedded in methacrylate, sectioned with a porter-blum microtome, and studied with a r.c.a. emu-2c electron microscope. each spermatid of a group developing synchronously is attached by a cytoplasmic strand to a common nutrient protoplasmic mass. this mass contains mitochondria and yolk bodies but is anucleate. the proximal centriole, that is, the centriole nearer the nucleus, is at first associated wi ...195913673045
cytological studies of the nematocysts of hydra. i. desmonemes, isorhizas, cnidocils, and supporting structures.entire hydras or tentacles were fixed in oso(4) or in kmno(4) and thereafter washed, dehydrated, and embedded in a methacrylate mixture. ultrathin sections were cut on an experimental model, thermal expansion type ultramicrotome or on a porter-blume microtome. the sections were examined in an rca electron microscope. type emu-2 d. "squash preparations" for light microscopy, were made from the hydra mouth region and the attached tentacles. these were observed with an ao baker interference microsc ...195913630936
[structure and function of the lacuna layer in the eggshell of the emu and cassowary]. 196013748080
surgeon apprentice and naturalist: being incidents from the journal of william l. crowther during the voyage of the barque "emu" from van diemen's land to london dock, february 24 to june 23, 1839. 196013813060
fine structure of the larval anuran epidermis, with special reference to the figures of eberth.small pieces of skin from 8 cm long rana clamitans larvae were fixed in oso(4), washed, dehydrated, and embedded in a methacrylate mixture. ultrathin sections were cut on a porter-blum ultramicrotome and were examined in an rca electron microscope, type emu 2d. the sections showed that aggregates of fibrous material in the cells of the inner layer of epidermal cells are identical in disposition and size with the classical figures of eberth. it is conclusively shown that these figures do not aris ...196113692395
arteriosclerosis in the ostrich, rhea, and emu: naturally occurring lesions of the aortas and coronary arteries. 196514254909
arteriosclerosis in the ostrich, rhea, and emu. 196514318834
a plastic handling tool for the rca emu-3 electron microscope specimen holders. 19676035436
[comparative studies on arteriosclerosis in wild and domestic animals. i. spontaneous arteriosclerosis in the emu, dromiceius novaehollandiae]. 19705462661
magnetic properties of the lunar crystalline rock and fines.magnetic measurements have shown that nondiamagnetic minerals in a lunar crystalline rock of type b are (free fe(2)+ in paramagnetic pyroxenes) : (antiferromagnetic fesio(3)) : (antiferromagnetic fetio(3)) : (ferromagnetic iron) = 4.3 : 7 : 20 : 0.08 in weight percentage. the abundance of ferromagnetic fe in the lunar fines is about 7.5 times its abundance in the crystalline rock. the natural remanent magnetization of the crystalline rock of 7.5 x 10(-6) emu/ g in intensity may not be attributab ...197017781554
magnetic properties of lunar dust and rock samples.determinations on 20-to 80-milligram portions of the rock samples and the -150 mesh fraction of the lunar dust show pronounced curie points between 680 degrees and 780 degrees c. remanent intensities of five rock fragments vary from 8.4 x 10(-5) to 0.30 x j0(-5) emu/gram. upon demagnetization, two of the samples had only viscous magnetization and two other samples had stable magnetizations with remanent coercivities in excess of 50 oersteds. partial thermal demagnetization suggests that these ap ...197017781549
the magnetic field induced by the bodily tide in the core of the earth.the motion in the liquid core of the earth due to the bodily tide can induce a periodic magnetic field having the frequency sigma of the tide as well as multiple frequencies, including a steady term. the coupling coefficient for the steady term between the convectively inducing and induced fields is estimated to be of the order of sigmah(2)/lambda, where h denotes the height of the equilibrium tide, and lambda = 1/4pikappa, kappa denoting the electrical conductivity of the core. with sigma = 1.4 ...197116591922
the complete amino acid sequence of a feather keratin from emu (dromaius novae-hollandiae). 19734577821
isolation and properties of a low molecular weight protein (apovitellenin i) from the high-lipid lipoprotein of emu egg yolk. 19734696759
primary structure of cytochrome c from the emu, dromaeus novaehollandiae. 19734349739
nuclear dna content of the emu. 19744140056
amino acid sequence of a feather keratin from silver gull (larus novae-hollandiae) and comparison with one from emu (dromaius novae-hollandiae). 19744429491
the amino acid sequence of a protein (apovitellenin i) from the low-density lipoprotein of emu egg yolk. 19744363370
immunoabsorption of membrane-specific antibodies for determination of exposed and hidden proteins in human erythrocyte membranes.human erythrocyte membrane proteins solubilized with the non-ionic detergent berol emu-043 have been characterized by crossed immunoelectrophoresis with rabbit antibodies raised against the membrane material. three out of sixteen membrane-specific immunoprecipitates disappeared when the antisera were first absorbed with intact erythrocytes. this finding indicates that three antigens are exposed on the outside of the erythrocyte membrane. one of these antigens showed acetylcholinesterase activity ...197549198
quantitative immunoelectrophoresis of proteins in human erythrocyte membranes. analysis of protein bands obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.1. we have defined conditions that permit quantitative immunoelectrophoresis in agarose gels of dodecyl sulfate-solubilized erythrocyte membrane proteins. 2. using human serum albumin, transferrin, mn-glycoprotein (glycophorin) and crude spectrin as test proteins, we found that accurate analyses are possible if samples and gels are 1% in non-ionic detergent (berol emu-043) or triton x-100) and if no more than 100 nmol free dodecyl sulfate is applied per sample. 3. dodecyl sulfate treated membran ...197552375
immunochemical analyses of membrane-bound complement. detection of the terminal complement complex and its similarity to "intrinsic" erythrocyte membrane proteins.(1) membranes of sheep erythrocytes lysed with antibody and human or rabbit complement were solubilized in non-ionic detergents (triton x-100 or berol emu-043) and analysed immunochemically using antisera directed against individual complement components. the precipitation behaviour of membrane-bound c3, c5, c6 and c9 components of complement was examined by immuno-double diffusion, rocket- and crossed immunoelectrophoresis performed in agarose gels containing 1% non-ionic detergent. (2) membran ...1975809065
studies on the apoproteins of the major lipoprotein of the yolk of hen's eggs. i. isolation and properties of the low-molecular-weight apoproteins.in a continuing study of protein-lipid interactions in egg yolk, the total apoprotein mixture (i.e. the 'apovitellenins') from the high-lipid, low-density lipoprotein (density 0.97 g/ml) of the yolk from hen's eggs has been isolated in a soluble form. by gel-filtration chromatography in 6m urea the mixture has been separated into several fractions from which three new low-molecular-weight proteins (i, ia, and ii), making up about 30% of the total, have been isolated. the most plentiful of these ...19751167127
reactivity and ionization constants of the lysine residues in apovitellenin i of emu egg yolk low-density lipoprotein by competitive labelling.competitive labelling with[14c]acetic anhydride over a range of ph values has been used to explore the surface topography of the apovitellenin i moiety in emu egg yolk low-density lipoprotein. the reaction of the lysine xi-amino groups with acetic anhydride has been related to ph in a set of titration curves; from these, the reactivities relative to alanine and the ionization constants of all but the amino terminal lysines have been determined. all lysines have near normal pka values around 10, ...19755069
primary structure of apovitellenin i from hen egg yolk and its comparison with emu apovitellenin i.the amino acid sequence of apovitellenin i from hen egg yolk has been determined using both automatic and manual procedures; it comprises 82 residues. hen apovitellenin shows considerable homology with emu apovitellenin i which contains 84 residues. besides two deletions in the sequence, the hen protein differs in 28 positions from the emu protein; 26 of these positions may have arisen from single base changes. the changes are largely conservative ones, which suggest that the structure and funct ...1976988817
response to co2 of intrapulmonary chemoreceptors in the emu.we studied discharge frequencies of 12 intrapulmonary chemoreceptors in the paleopulmonic lung of an emu (dromiceius novaechollandiae) during unidirectional, artificial ventilation when step changes and static co2 concentrations were given. discharge frequency in afferent neurons from the receptors increased as intrapulmonary co2 decreased. the response of the receptors to various static intrapulmonary co2 concentrations was similar to that previously demonstrated for the duck and chicken. the m ...19761019429
chemoreceptors in the paleopulmonic lung of the emu: discharge patterns during cyclic ventilation. 19761013516
editorial: dr. edwards' emu. 1976937161
calf thymus alkaline phosphatase. ii. interaction with detergents.1. a number of detergents were used to dissolve calf thymus plasma membranes rich in alkaline phosphatase (orthophosporic-monoester phosphohydrolase (alkaline optimum), ec 3.1.3.1) activity. 2. the stokes' radius (r) of alkaline phosphatase in each detergent was measured by gel filtraton. the size of the solubilized enzyme varied from r = 6.2 nm in sodium cholate to r = 8.3 nm in berol emu-043. with n-alkylsulphates, the apparent size increased with alkyl chain length, with r = 6.4 nm (c9) and ...1977831832
lipid-protein globules of avian egg yolk. isolation and properties of globules stable in concentrated sodium chloride solution.a new type of globular particle, the 'insoluble yolk globule', was isolated from the egg yolk of three avian species (hen, duck, and emu) by centrifugation or gel-filtration chromatography. these globules are stable in nacl and urea solutions at concentrations that dissolve or disrupt other constituents of yolk, the isolated globules are about 1% of the dry yolk of hen's and duck's eggs but about 8% emu's-egg yolk. most of these globules are less than 2 micrometer in diameter. electron micrograp ...1977563717
transient magnetic effects in a scale model of the earth's core.scale model experiments are described in which the contribution to the dipole magnetic field of a conducting sphere (simulating the earth's core) by initially closed, internal flux loops is measured. a magnetic loop is maintained by constant current in a toroidal coil, in a mercury sphere. the circuit is then opened, which allows the magnetic loop to diffuse and dissipate. the time development of the magnetic effects outside the sphere is recorded, especially the contribution in the dipole mode ...197716592464
integrated circuit ac mutual inductance bridge for magnetic susceptibility measurements.a versatile ac mutual inductance bridge is described which uses integrated circuit operational amplifiers (op-amps) for the measurement of magnetic susceptibilities of ferro-, ferri-, and para-magnetic samples. the circuit employs op-amps both for balancing the bridge and for detecting the differential signal from the sample coil. the response of the circuit is linear over a wide range of sample susceptibilities, and is calibrated directly in absolute units (emu) using a multiturn digital potent ...197818699301
urinary hydroxyproline excretion in carcinoma of the prostate. a comparison of 4 different modes of assessment and its role as a marker.urinary hydroxyproline (hp) excretion has been estimated without prior dietary restriction in 33 patients with carcinoma of the prostate and expressed as either 24-h hp output or as the hydroxyproline/creatinine (hp/cr) ratio in 24-h urine sample, an early morning urine sample or a spot urine sample. the early morning urine hydroxyproline/creatinine ratio (emu hp/cr) appears to be the most accurate and avoids the disadvantages of formal dietary restriction and prolonged urine collection. the res ...1979534843
the epididymis and its development in ratite birds (ostrich, emu, rhea).the epididymis of ratitae is subdivided into a main part and a appendix epididymidis. the appendix epididymidis consists of the ductus aberrans and ductuli aberrantes. the ductus aberrans is the cranial continuation of the ductus epididymidis. the appendix epididymidis is cranially attached to the adrenal gland. in the main part of the epididymis the largest part of the rete testis is found. the rete testis is composed of an intratesticular rete (also named tubuli recti), and intracapsular rete ...19817270904
the horizontal magnetic dance of the honeybee is compatible with a single-domain ferromagnetic magnetoreceptor.although honeybees are able to sense the geomagnetic field, very little is known about the method in which they are able to detect it. the recent discovery of biochemically precipitated magnetite (fe3o4) in bees, however, suggests the possibility that they might use a simple compass organelle for magnetoreception. if so, their orientation accuracy ought to be related to the accuracy of the compass, e.g. it should be poor in a weak background fields and enhanced in strong fields. when dancing to ...19817295911
the bursa cloacae (fabricii) of struthioniforms in comparison with the bursa of other birds.the late embryonic and postembryonic genesis of the bursa cloacae (fabricii) of struthioniforms and other birds is described and discussed. the bursa of ostrich and emu is a wall organ of the caudal cloacal chamber. the bursa of rhea is, like the bursa of gallus, a cranial appendix of the proctodeum. lobuli bursales of struthioniforms are composed of a peripheral pars lymphoepithelialis (ple) and a central pars lymphoreticularis (plr). by contrast, lobuli bursales of gallus are composed of a per ...19827077679
pharmacokinetics and tissue concentrations of tylosin in selected avian species.tissue and plasma concentrations and the biological half-life of tylosin in avian species of a variety of body sizes and metabolic rates were studied. the species chosen were eastern bobwhite quail (colinus virginianus virginianus), pigeons (columba livia), greater sandhill cranes (grus canadensis tabida), and emus (dromaius novaehollandiae). in the 1st phase of this study, tylosin was administered im to quail, pigeons, and emus at a dosage rate of 25 mg/kg of body weight and to cranes at a dosa ...19827149381
the measurement of urinary steroid glucuronides as indices of the fertile period in women. world health organization, task force on methods for the determination of the fertile period, special programme of research, development and research training in human reproduction.the concentrations of various estrogen glucuronides and pregnanediol-3 alpha-glucuronide have been measured in daily samples of early morning urine (emu) and 24 h pooled collections throughout a total of 70 menstrual cycles (58 subjects). the immediate objective was to identify and assess the potential value of measuring hormone metabolites to locate the fertile period in women, with the ultimate aim of developing a non-invasive immunochemical test. the project was undertaken in five centres and ...19827176659
hypothalamic thermosensitivity in an emu, dromiceius novae-hollandiae. 19827099926
the effect of acetazolamide on the proteinuria of altitude.albumin was measured by dipstick tests and immunologically in 24-h and early morning urine (emu) samples collected from 20 subjects during a high-altitude trek. each was given acetazolamide (diamox sustets) or placebo as part of a double-blind trial on the prophylactic use of acetazolamide in acute mountain sickness (ams). at the highest altitudes, albuminuria was six times greater in those on placebo (p less than 0.001) and was related to the clinical features of ams (p less than 0.01) and arte ...19827034715
amino-acid sequence of feather keratin from fowl.the amino acid sequence of a single polypeptide chain, b-4, from fowl feather barbs has been determined. the b-4 chain was found to consist of 96 amino acid residues and to have a molecular weight of 10206 in the s-carboxymethylated form. the n terminus of this protein was an n-acetylserine residue. the b-4 protein contained seven s-carboxymethylcysteine residues, six of which are located in the n-terminal region (residues 1-26), and other one in c terminus. the central region of the peptide cha ...19836189711
the effect of two chemical mutagens enu and mms on mr-mediated reversion of an insertion-sequence mutation in drosophila melanogaster.the effects of two mutagens enu and mms characterized by different alkylation patterns have been studied on the reversion of an mr-induced singed mutation to wild-type. reversion of this unstable singed mutation under the influence of mr is assumed to represent the removal or transposition of an insertion element. since mr acts primarily in spermatogonia, the mutagens were fed to 1st instar larvae. recessive lethal tests were carried out simultaneously to calibrate for the mutagenic effectivenes ...19836308435
panting in the emu causes arterial hypoxemia.the purpose of this study was to determine the effect of heavy thermal panting on arterial oxygen (pao2) and carbon dioxide (paco2) tension in emus. the birds showed no significant change in body temperature during a 3-4 h heat stress caused by increasing ambient air temperature from 21 to 46 degrees c. however, the emus increased their respiratory frequency 10-fold (from 5.3 to 52.9 breaths x min-1). the high respiratory frequency resulted in a slight but significant decrease in paco2 (from 33. ...19836420859
cardiovascular changes in the exercising emu.cardiovascular variables were studied as a function of oxygen consumption in the emu, a large, flightless ratite bird well suited to treadmill exercise. at the highest level of exercise, the birds' rate of oxygen consumption (vo2) was approximately 11.4 times the resting level (4.2 ml kg-1 min-1). cardiac output was linearly related to vo2, increasing 9.5 ml for each 1 ml increase in oxygen consumption. the increase in cardiac output is similar to that in other birds, but appears to be larger th ...19836875470
bacterial endocarditis and thromboembolism of a pelvic limb in an emu. 19846511604
some histochemical properties of the fiber types in the pectoralis muscle of an emu (dromaius novaehollandiae).the muscle fibers of the cranial slip of m. pectoralis pars thoracica of an emu (dromaius novaehollandiae) were studied histochemically for intracellular lipid, succinic dehydrogenase, myofibrillar adenosine triphosphatase, and acetylcholinesterase. it was concluded that the muscle consisted of approximately 28% slow-tonic and 72% fast-twitch glycolytic fibers. the tonic fibers were considered to be characteristic of a postural muscle, and the fast-twitch glycolytic fibers to reflect the inabili ...19846235756
direct assay of urinary steroid glucuronides for monitoring the approach of ovulation.the concentrations of estrone-3-glucuronide (e(1)3g) and pregnanediol-3-glucuronide (p(2)3g) in daily samples of early morning urine (emu) were correlated with the levels of estradiol (e2), progesterone (p) and lh in respective plasma samples. forty-six menstrual cycles were studied, in order to determine the practical usefulness of the urine assays for detecting: a) an individualized estrogen concentration threshold value, announcing the approach of ovulation. b) an individualized signal provid ...19846152260
the measurement of urinary lh, by a solid-phase chemiluminescence immunoassay.ovulation in women may be predicted or detected by a simple, solid-phase, chemiluminescence immunoassay for the measurement of urinary lh. an igg fraction of a donkey antiserum to rabbit immunoglobulins is passively adsorbed to the walls of polystyrene tubes. human chorionic gonadotrophin-succinyl-butyl-ethyl-isoluminol and a primary antibody (rabbit anti-human lh) is incubated with samples of early morning urine. after the binding reaction, the solution is removed by aspiration. the antibody-bo ...19846385812
ratites as oldest offshoot of avian stem--evidence from alpha-crystallin a sequences.one of the most disputed issues in avian phylogeny is the origin of the ratites, the large flightless birds of the southern hemisphere (reviewed in refs 1-3). it is still not generally agreed whether the ostriches, rheas, emus and cassowaries, and probably kiwis, form a natural, monophyletic group, although much recent evidence supports this view. also, their phylogenetic relationship with the other avian orders remains unresolved, comparative protein sequence studies might shed new light on thi ...19846482951
multimeric analysis of von willebrand factor in megakaryocytes.von willebrand factor (vwf) is a glycoprotein that appears to play a major role in subserving the adhesion of platelets to subendothelium during hemostasis. endothelial cells have been shown capable of synthesizing and releasing this large, multimeric glycoprotein that normally circulates in the plasma in association with the factor viii coagulant molecule. megakaryocytes, the precursor cells of blood platelets, also appear to possess vwf biosynthetic capacity, since cultured guinea pig megakary ...19853875163
osmotic and ionic regulation during dehydration in a large bird, the emu (dromaius novaehollandiae): an important role for the cloaca-rectum.the emu, a large (40 kg) flightless bird, has low water requirements yet it has a limited ability to produce concentrated urine. to clarify the factors involved in excretion and water conservation by the emu the patterns of excretion during water restriction were examined. additionally, kidney function was studied, as also was the role of the cloaca and rectum in the final modification of excreta. following water restriction the osmolality of the excreta fluid increased to a maximum level at 6 d ...19854034920
use of superparamagnetic particles for isolation of cells.this report describes the preparation and characterization of synthetic ferritin-like particles produced by precipitation of magnetite from a mixture of ferrous and ferric ions in the presence of dextran. the 3-nm diameter particles, containing magnetite cores surrounded by chemisorbed dextran, had a magnetization of 46.7 emu/g of iron with mössbauer quadrupole splitting of 2 delta = 0.76 mm/s. the application of these particles as a laboratory reagent for isolation of legionella from other wate ...19862418094
magnetite and magnetotaxis in algae.magnetotactic algae of the genus anisonema (euglenophyceae) have been isolated from a coastal mangrove swamp in northeastern brazil. the magnetotactic response is based on a permanent magnetic dipole moment per cell approximately 7 10(-10) emu. each cell contains many magnetite (fe(3)o(4)) particles organized in chains.198619431684
prediction and detection of ovulation by the measurement of urinary pregnanetriol-3 alpha-glucuronide. a multicentre study.the urine excretion pattern of pregnanetriol 3 alpha-glucuronide (pt-3g) throughout the menstrual cycle in 26 normal ovulating women was evaluated in a multicentre study. the concentration of pt-3g was measured by radioimmunoassay in daily samples of early morning urine (emu) from 20 women for three consecutive cycles and from 6 women who conceived during the period of study. pt-3g was also measured in 24-h urine samples from 5 additional women. the peak of urine lh was used as a reference point ...19863702465
[mesonephric origin of steroid hormone producing interrenal nodes in the epididymis of the emu (dromaius novaehollandiae)]. 19873481511
[iatrogenic inguinal injury of the spermatic cord from a treatment and forensic viewpoint].iatrogenic obstruction of the spermatic duct or iatrogenic occulusive azospermia, in a wider context, is more frequently diagnosed today than it used to be in the past. this is attributable to general improvement of the system of andrological care. hints are given on avoidance of that lesion by surgical tactics as well as on provisional treatment and on compulsory notification of injuries that occurred to the spermatic duct. a rating of 20 to 30 per cent of disablement is proposed for irreversib ...19873591044
occurrence of magnetic material in teleosts.fishes representing the main groups of teleosts have been investigated for magnetic material by susceptibility measurements. all the investigated species contain magnetic material. bone samples from the skull and the vertebral column contain magnetic particles which yield a saturation magnetization in the range 10(-4) emu g-1 to 10(-3) emu g-1 in a sample. the localization of the magnetization is diffuse within the tissues connected to the bone. there are no significant differences between the a ...19872881648
the amino acid sequence of emu osteocalcin: gas phase sequencing of gla-containing proteins.osteocalcin (oc), the major gamma carboxyglutamic acid (gla)-containing protein of vertebrate bone, has been isolated from bones of the emu (dromaius novaehollandae) and the primary structure determined by a combination of gas phase n-terminal sequencing of the intact molecule and a proteolytic fragment, and carboxypeptidase y c-terminal sequencing. gla residues were located by counting tritium radioactivity in fractions from the n-terminal sequencing of the tritiated/thermally decarboxylated mo ...19873501719
lymph heart musculature in birds.development and innervation of the lymph heart musculature of chicken, emu, rhea, and duck was studied by electron microscopy at post-hatch ages from 3 days to adulthood. development of innervation was monitored by acetylcholinesterase staining. horseradish peroxidase was used to determine the extent of the transverse tubule network. chickens were unusual among these birds in that lymph heart myocytes had already undergone a definitive differentiation and degeneration by 3 days. in ducks and rat ...19873820312
specific guidance of motor axons to duplicated muscles in the developing amniote limb.the effect of alteration of limb pattern upon motor axon guidance has been investigated in chick embryos. following grafting of the zone of polarizing activity (zpa) into the anterior margin of the early limb bud, limbs develop with forearms duplicated about the anteroposterior axis. the position of motoneurones innervating the duplicated posterior forearm extensor emu was mapped by retrograde transport of horse radish peroxidase (hrp). the motor pool labelled from injection into the anteriorly ...19883197635
simplified quantification of urinary protein excretion in children.the protein/creatinine ratio (up/uc) measured in 71 early morning urine samples (emu) correlated closely with timed overnight urine (onu) protein excretion rates (r = 0.96). the relationship was linear throughout the entire range of normal and abnormal protein excretion, an onu rate of 1 mg/h/m2 body surface area being proportional to an emu up/uc of 5 mg/mmol. using the coomassie blue dye-binding method the upper limit of up/uc in 377 apparently healthy children and adolescents aged 3-19 years ...19883214967
hemopoietic lineage switch: v-raf oncogene converts emu-myc transgenic b cells into macrophages.hemopoietic lineage commitment can be breached by concomitant expression of the c-myc and v-raf oncogenes. switching to the myeloid lineage occurred frequently when b lineage cells, from either lymphomas or preleukemia bone marrow cells of emu-myc transgenic mice, were infected with a retrovirus bearing v-raf. cloned pre-b and b cell lines changed into either mature or immature macrophages as assessed by morphology, adherence, phagocytic activity, surface markers, and lysozyme production, but re ...19882454746
genetic studies on emu-myc transgenic mice. 19883265095
properties of the liquid crystals of some biopolymers.nematic liquid-crystalline solutions of polypeptides such as pblg and pelg orient in the direction of a very low static electric field as if the polypeptide molecules were a collection of rod-like molecular clusters having a huge, permanent electric dipole moment. this dipole moment originates from fluctuation of the distribution of polymer helices in both directions of the rod-like molecular cluster with roughly equal likelihood. the molecular cluster of pblg is estimated to be composed of some ...19883077236
the lung of the emu, dromaius novaehollandiae: a microscopic and morphometric study.qualitative and quantitative characteristics suggest that the lung of the emu is poorly adapted for gas exchange when compared with that of other birds. the granular epithelial cells extend over the air capillaries, and the squamous epithelial cells have microvilli indicating a poor differentiation of the epithelium of the exchange tissue. the surface area of the blood-gas tissue barrier per unit body mass was only 5.4 cm2/g, the volume of the pulmonary capillary blood per unit body mass was onl ...19892606782
oncogene cooperation and b-lymphoid tumorigenesis in emu-myc transgenic mice. 19892696688
acceleration of b-lymphoid tumorigenesis in e mu-myc transgenic mice by v-h-ras and v-raf but not v-abl.emu-myc transgenic mice constitutively express the c-myc oncogene from before birth, but their inexorable development of b-lymphoid tumors appears to require rare spontaneous alterations within b-lineage cells. to determine whether other oncogenes can cooperate with myc to induce b-lymphoid transformation, we infected neonatal emu-myc mice and normal littermates with helper-free retroviruses bearing the v-h-ras, v-raf, or v-abl oncogene. the v-h-ras and the v-raf oncogene dramatically accelerate ...19892549487
lymphomagenesis in emu-myc transgenic mice does not require transgene rearrangement or mutation of myc exon 1.in most human burkitt's lymphomas, translocation of the myc oncogene to an immunoglobulin locus is associated with loss of myc exon 1 or with mutations near its 3' border, a region where myc transcription is attenuated and translation of a larger myc polypeptide initiates. emu-myc transgenic mice, which bear the three myc exons coupled to an immunoglobulin enhancer, provide a model for the development of such lymphomas, because their lymphomagenesis appears to require events other than expressio ...19892699507
restricted utilization of vh and dh genes in leukemic rabbit b cells.polyclonal b cell leukemias have been generated in 17- to 20-day old emu-myc transgenic rabbits. to analyze the repertoire of vh genes utilized in early b cells, eight vdj genes were cloned from these leukemic cells. the nucleotide sequences of these genes indicated that seven of the eight vdj genes encoded prototype vha1, vha2 or vha3 allotypes. the two vdj genes encoding vha1 molecules had vh segments with identical nucleotide sequences; similarly, the vh segments of the four vdj genes encodin ...19902107085
partial amino acid sequence of osteocalcin from an extinct species of ratite bird.osteocalcin the major gamma carboxyglutamic acid containing protein of vertebrate bone has been purified from the bones of a specimen of pachyornis elephantopus, a species of the extinct class of new zealand ratite birds, the moas. the sequence of the n-terminal region of moa osteocalcin was determined using gas phase n-terminal sequencing. the n-terminal sequences of the ostrich and rhea osteocalcins were also determined. alignment of the n-terminal sequence of osteocalcin from the extinct moa ...19902222494
abelson murine leukemia virus transforms preneoplastic emu-myc transgene-carrying cells of the b-lymphocyte lineage into plasmablastic tumors.e mu-myc transgenic mice were back-crossed to balb/c mice up to back-cross generation 3. the offspring that included transgene-carrying and -negative mice in approximately equal proportions were randomly divided into 2 groups. thirty-four mice (group i) were treated with pristane, followed by a-mulv, and 40 (group ii) were injected with a-mulv alone. altogether, 16 lymphoid tumors developed in group i and 17 in group ii. nine of the tumors in group i and 4 in group ii appeared as ascitic tumors. ...19902228313
rearrangement of vha1-encoding ig gene segment to the a2 chromosome in an a1/a2 heterozygous rabbit. evidence for trans recombination.vdj genes were cloned from leukemic b cells of an a1/a2 heterozygous emu-cmyc transgenic rabbit. restriction mapping and nucleotide sequence analysis indicated that one clone, 5c3, had a vha1-encoding gene segment functionally rearranged to a jh gene segment from the a2 chromosome. this vdj gene may be the result of a trans recombination between a vh gene on the a1 chromosome and a jh gene segment on the a2 chromosome or, it may be the result of a cis recombination if the a2 chromosome contains ...19902307849
selection of the t-cell repertoire in transgenic mice expressing a transplantation antigen in distinct thymus subsets.transgenic mice that expressed a transplantation antigen, h-2kb, in an unusual tissue distribution have been developed. gene-regulatory elements from the immunoglobulin heavy-chain locus (emu enhancer and heavy chain promoter) were linked to the class i kb gene and the construct microinjected into fertilized mouse eggs of a different haplotype. it was expected that such gene-regulatory elements would direct expression of the foreign class i molecules only to b and t lymphocytes. however, express ...19901979441
igh enhancer deregulated expression of l-myc: abnormal t lymphocyte development and t cell lymphomagenesis.transgenic constructs containing the murine l-myc gene under the control of the immunoglobulin transcriptional enhancer element (emu) are expressed at unexpectedly high levels in thymocytes and proliferating t cells compared with cells from bone marrow and proliferating b cells. in contrast, double transgenic animals bearing constructs containing the l- and n-myc genes similarly linked to the emu element maintain preferential l-myc expression in t cells but express the n-myc transgene preferenti ...19902120050
urinary hormonal profile during the first cycle of low-dose oral contraceptive pills in women.ovarian function was studied in ten normal fertile women before and during the first cycle on a low-dose micropill containing 30 mcg ethinyl estradiol and 150 mcg l-norgestrel. in a control cycle and the first treatment cycle, steroid metabolites estrone-3-glucuronide (e1-3g) and pregnanediol-3 alpha-glucuronide (pd-3g) were measured in daily early morning urine (emu) samples. also, luteinizing hormone (lh) was estimated during the expected periovulatory period. during the first cycle of micropi ...19902387153
neuron type processor modeling using a timed petri net.the basic operation of a digital neuron is reviewed, and the theory of time petri nets used for modeling, representation, and analysis of the neuron-type processor (ntp) is reviewed. the timed petri net is utilized to produce a model for the digital ntp. the neuron-type processor performs input temporal and spatial summation, as well as thresholding. the timed petri net of the ntp operates asynchronously and sequentially takes on a series of distinct internal states, so that each of these states ...199018282848
a study of an alcohol advertisement for low alcohol beer.a study was undertaken to measure the blood alcohol levels (bals) of five men and five women after they had consumed seven middies of emu 2.2 (2.2% alcohol by volume) beer in 1 hour. this study was conducted in response to a newspaper advertisement which depicted a well-known football player drinking seven middies of emu 2.2 beer over 1 hour and registering a bal of 0.012%. as predicted, the bals of the ten adults varied considerably, but all greatly exceeded the very low result obtained by the ...199016840144
the epidermal melanin unit in the pathophysiology of malignant melanoma.the epidermal melanin unit (emu) denotes the symbiotic relationship between a melanocyte and a pool of associated keratinocytes. we propose to show that alterations in the biology of the emu are the main determinant of the different patterns of intraepidermal growth of melanocytes in lentigo maligna melanoma (lmm) and superficial spreading melanoma (ssm). they also appear to affect the biosynthesis of melanin and melanosomes during malignant transformation. findings in histochemical studies with ...19912029092
carcinogen-induced lymphomagenesis in pim-1 transgenic mice: dose dependence and involvement of myc and ras.transgenic mice overexpressing the pim-1 oncogene in their lymphoid compartments are predisposed to t-cell lymphomagenesis but only to the extent that approximately 10% of the transgenic mice develop lymphomas within 34 weeks after birth. recently, we have shown that lymphomagenesis in pim-1 transgenic mice can be accelerated by infecting pim-1 transgenic mice with murine leukemia viruses or by treating the mice with a relatively low dose of 60 mg of the carcinogen n-ethyl-n-nitrosourea (enu) pe ...19911988138
sequence conservation in avian cr1: an interspersed repetitive dna family evolving under functional constraints.cr1 is a short interspersed repetitive dna element originally identified in the domestic chicken (gallus gallus). however, unlike virtually all other such sequences described to date, cr1 is not confined to one or a few closely related species. it is probably a ubiquitous component of the avian genome, having been detected in representatives of nine orders encompassing a wide spectrum of the class aves. this identification was made possible by using the polymerase chain reaction (pcr), which rev ...19911829530
spermatozoa and relationships in palaeognath birds.in this paper the authors describe the ultrastructure of the mature spermatozoon and the spermatid in struthio camelus and dromaius novaehollandiae. the first species is characterized by a rod-like perforatorium within an endonuclear canal in the anterior third of the nucleus, while the second is characterized by an extremely reduced completely extranuclear perforatorium. other differences are in the sperm dimensions, the number of mitochondria and the length of the axonemal accessory fibers. co ...19911912945
evaluation of endocrine parameters in clinical trials with beta-hcg vaccine.this report presents data on various endocrine parameters with respect to pituitary-ovarian axis in-depth in control vs 18 subjects immunized with three beta-hcg based vaccine formulations. hormonal parameters such as tsh, prl, acth, progesterone, cortisol, t3 and t4 were measured by ria in sera in control pre-vaccine cycles as well as at 3, 6, and 12 months after primary vaccine immunization. in 6 women urinary e1g, pdg, lh and fsh were measured by elisa/ria tests on early morning urine (emu) s ...19911706244
activation of immunoglobulin control elements in transgenic mice.to assess the role interleukins and mitogens play in regulating immunoglobulin (ig) gene expression via the ig enhancer and promoter, transgenic mice carrying two different ig gene regulatory regions were generated. one, emukcat, contains the ig heavy chain enhancer (emu) and the kappa light chain promoter driving the chloramphenicol acetyltransferase (cat) gene. in the other, delta emukcat, cat is under the control of the kappa promoter alone. emu and kappa relative activity were assessed by ca ...19921729170
eastern equine encephalitis in a flock of emus (dromaius novaehollandiae).eastern equine encephalitis (eee) was diagnosed in a flock of emus in southeastern louisiana. the outbreak involved juvenile and adult breeders ranging in age from 20 to 36 months, with an attack rate of 76% and a case fatality rate of 87%. the diagnosis was confirmed by isolation and characterization of the viral agent, and by detection of eee antibody in two recovered emus. high mortality was preceded by marked depression, hemorrhagic diarrhea, and emesis of blood-stained ingesta. on postmorte ...19921417619
reference serum biochemical values for emus and ostriches.reference serum biochemical values were determined in blood samples from 15 male, 18 female, and 4 unsexed emus (dromaius novaehollandiae) 1 to 48 months old. serum biochemical values also were obtained for 19 male, 26 female, and 4 unsexed ostriches (struthio camelus) 1 to 60 months old. parametric (mean +/- 2 sd) and non-parametric (fifth to 95th percentile) reference ranges and linear trends as influenced by age were determined for enzyme activities and concentrations of glucose, inorganic ph ...19921456516
strain dependency of cell-type specificity and onset of lymphoma development in emu-myc transgenic mice.c-myc is a nuclear proto-oncogene that, when activated, induces malignancies in a variety of tissues. most murine plasmacytomas and human burkitt's lymphomas have been shown to carry a chromosomal translocation involving c-myc and immunoglobulin genes. to study genetic or epigenetic factors that affect myc-induced lymphoid cell tumors, we previously introduced the emu-myc delta gene lacking its own promoter and first exon into two inbred strains of mice, c57bl/6 and c3h/hej. we observed three ch ...19921582889
transient expression of the coat protein of sugarcane mosaic virus in sugarcane protoplasts and expression in escherichia coli.the coat protein (cp) of strain sc of sugarcane mosaic virus (scmv-sc) was expressed transiently in sugarcane protoplasts after electroporation with one of two plasmids encoding the cp gene. the cp gene was fused with either the cauliflower mosaic virus 35s promoter or the synthetic monocotyledon promoter "emu". the coat protein gene was also inducibly expressed in escherichia coli when fused to the trc promoter. the protein expressed in both systems had the same electrophoretic mobility and ant ...19921642548
down-regulation of c-myc antigen expression in lymphocytes of emu-c-myc transgenic mice treated with anti-c-myc dna methylphosphonates.in transgenic mice bearing a murine immunoglobulin enhancer/c-myc fusion transgene (emu-myc), it was found that antisense dna methylphosphonates targeted against c-myc mrna inhibited production of c-myc protein in peripheral lymphocytes. the decrease in protein was measured 3-4 h after i.v. administration of a 300-nmol dose. c-myc was detected by immunofluorescence of fixed cells stained with an anti-c-myc antiserum. in addition, dna methylphosphonates did not induce acute toxicity following i.v ...19921458461
inhalation doses for aboriginal people reoccupying former nuclear weapons testing ranges in south australia.the australian government plans to rehabilitate the former nuclear weapons testing ranges at maralinga and emu in south australia and to allow access to the areas for the pitjantjatjara (aboriginal) people who are the traditional owners of the land. the major radiological hazard posed by reoccupation of the ranges arises from inhalation of residual plutonium. the committed effective dose that these people might receive from inhalation on reoccupation of the lands is computed for a range of sites ...19921428883
assessment of potential doses at the maralinga and emu test sites.an assessment has been undertaken of potential doses to future aboriginal inhabitants of the maralinga and emu areas of south australia, where nuclear weapons tests in the 1950s and 1960s have resulted in residual radioactive contamination. radioactive material due to this program of tests and other experiments is still detectable several tens of kilometers from some of the test sites. continued occupancy by individuals following an aboriginal lifestyle could give rise to annual effective dose e ...19921428882
urinary protein/creatinine index in follow up of patients with wilms' tumour after nephrectomy.the protein/creatinine index (p/c) was determined in early morning urine (emu) samples from available patients with wilms' tumour who had had a nephrectomy and whose diagnosis had been made between january 1970 and december 1989. clinical details were obtained by case note review. results were obtained from 36 boys and 40 girls. the mean interval between nephrectomy and measurement of the emup/c was 9.0 years (2-23). eleven patients had a emup/c greater than 20 mg/mmol (normal range less than 20 ...19921336953
immunochemical tests of potential fertility.there are many methods of determining the physiological basis of cyclical periods of potential fertility: calculations, sensations, or physical or chemical biosensors. this article reviews the development of self-tests of potential fertility using immunoassay of hormone metabolites in urine. indices of potential fertility in urine are the concentration of principal metabolites of steroid hormones and luteinizing hormone (lf). the ovaries produce an estimated 50-800 mcg of estradiol/day, the most ...19921633952
urinary tuberculosis: experience of a teaching hospital in syria.this study was designed to show our experience of urinary tuberculosis in one of the large teaching hospitals in syria. it was a prospective study involving 48 patients (29 males and 19 females) with confirmed tuberculous lesions in the urinary tract. the study period was between 1982-1987. the presenting symptoms were protean, and there were often delays between onset of symptoms and eventual diagnosis. the highest age incidence was in the second and fourth decades. beside the suggestive clinic ...19921459824
effects of promoter, intron and enhancer elements on transient gene expression in sugar-cane and carrot protoplasts.various chimaeric promoter regions coupled to the uida beta-glucuronidase gene were evaluated for transient expression strength following electroporation into sugar-cane (monocot) and carrot (dicot) protoplasts. multiple enhancer elements increased expression in sugar-cane, by up to 400-fold for the artificial emu promoter relative to the camv 35s promoter. the relative expression strengths of promoters varied substantially between the species. sugar-cane also differed in some respects from prev ...19938219094
effects of tissue type and promoter strength on transient gus expression in sugarcane following particle bombardment.effects of tissue type and promoter strength on transient gus expression in the sugarcane (saccharum spp. hybrids) cultivar nco 310 were evaluated following microprojectile bombardment of leaf explants. gus expression was histochemically or fluorometrically measured 48 h after delivery of the uida gene. high levels of gus expression were obtained in leaf segments isolated from young, expanding sugarcane leaves cultured for 1, 3, or 6 d prior to bombardment. the promoter derived from the maize ub ...199324201961
age-related changes in plasma biochemical values of farmed emus (dromaius novaehollandiae).blood samples were collected from 40 emus (dromaius novaehollandiae) of 4 different age groups ranging from 1 week to 14 months. plasma values of glucose, cholesterol, uric acid, total protein, albumin, creatine kinase, aspartate amino transferase, alkaline phosphatase, calcium, phosphorus and magnesium were measured. fourteen-month-old birds had lower plasma glucose values and enzyme activities and higher plasma protein values than younger birds. one-week-old birds had higher cholesterol and ur ...19938240173
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