Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| the effect of water acidification on prolactin cells and pars intermedia pas-positive cells in the teleost fish oreochromis (formerly sarotherodon) mossambicus and carassius auratus. | although exposure to acid water (ph 3.5) induces severe and prolonged reduction in plasma osmolarity and total plasma calcium concentration in tilapia (oreochromis mossambicus) and goldfish (carassius auratus), the responses of the hypophyseal cells are clearly different. in tilapia, the size of the rostral pars distalis of the pituitary gland is enlarged as a result of the increase in size and number of prolactin cells. the pars intermedia pas-positive (pipas) cells are not noticeably changed. ... | 1984 | 6525622 |
| some observations on the influence of stress on ecg patterns in oreochromis mossambicus and cyprinus carpio. | electrocardiograms of undisturbed, resting fish as well as disturbed (stressed) fish were recorded by biotelemetry. both fish species showed marked changes in heartbeat patterns in response to stress with tilapia exhibiting the most pronounced changes. a theory is advanced to account for the marked changes in electrocardiogram patterns of disturbed fishes. | 1985 | 2866875 |
| a thrombelastographic study of the effect of stress on the blood coagulation in cyprinus carpio (cyprinidae) and oreochromis mossambicus (cichlidae). | the normal blood coagulation in cyprinus carpio and oreochromis mossambicus was investigated by thrombelastography and standard thrombelastographs were obtained for each species. the effect of stress on blood coagulation was thereafter determined. the thrombelastograms indicated hypercoagulability under stress conditions. a prominent observation is the differences in the elasticity of the clot under various conditions. fibrinolysis occurred in all cases, except in the case of cyprinus carpio aft ... | 1985 | 2864206 |
| urotensin ii-immunoreactive neurons in the caudal neurosecretory system of freshwater and seawater fish. | antiserum generated against synthetic urotensin ii of the goby, gillichthys mirabilis, was used to localize urotensin ii in the caudal neurosecretory system in six species of freshwater teleosts: cyprinus carpio, carassius auratus, oreochromis mossambicus, oreochromis niloticus, salmo gairdneri and plecoglossus altivelis, and six species of seawater teleosts: acanthogobius flavimanus, pagrus major, parapristipoma trilineatum, trachurus japonicus, seriola dumerili and seriola quinqueradiata. in t ... | 1985 | 3884162 |
| effects of ovine prolactin on calcium uptake and distribution in oreochromis mossambicus. | ovine prolactin stimulated the net uptake rate of ca2+ from the water by 96%, produced frank hypercalcemia, and increased total bone calcium content in fed rapidly growing freshwater male tilapia, oreochromis mossambicus. it did not, however, alter the size of the readily exchangeable bone calcium pool. the increase in calcium accumulation resulted from an increase in whole-body ca2+ influx and a decrease in ca2+ efflux. it is concluded that prolactin exerts an important control over ca2+ exchan ... | 1986 | 3946634 |
| metabolism of isolated fish gill cells: contribution of epithelial chloride cells. | gill cell suspensions from freshwater (fw)- and seawater (sw)-adapted teleosts were obtained by density gradient centrifugation. the proportion of chloride cells (ccs) in the mixed cell suspensions was estimated using the fluorescent mitochondrial stain, daspmei, and ranged from less than 1% (fw-adapted tilapia) to approximately 13% (sw-adapted toadfish). the gill cells displayed relatively high viability based on trypan blue exclusion (greater than 75%), lactate dehydrogenase leakage (less than ... | 1989 | 2549165 |
| serotonergic neurons in the intestine of two teleosts, carassius auratus and oreochromis mossambicus, and the effect of serotonin on transepithelial ion-selectivity and muscle tension. | the application of an antiserum directed against rat serotonin demonstrated serotonin-immunoreactive cell bodies and varicose nerve fibres in the myenteric plexus of both goldfish and tilapia. in the circular muscle layer immunoreactive varicose nerve fibres and a few cell bodies could be detected. a fine network of varicose fibres was observed underlying the epithelial cells. serotonin immunoreactivity was not observed along incoming mesenterial blood vessels, suggesting that the serotonergic n ... | 1989 | 2594202 |
| chronic inflammatory cells with epithelial cell characteristics in teleost fishes. | certain cells that participate in the chronic inflammatory response of teleost fishes have many features typical of epithelioid cells of mammals. such features include high metabolic activity, frequent phagolysosomes, and cytoplasmic interdigitations between adjacent cells; however, the epithelioid granulomas formed in response to certain diseases in teleost fishes also have several features associated with epithelial cells. cases of ulcerative mycosis or acid-fast bacterial infection in atlanti ... | 1989 | 2686148 |
| possible involvement of polysialogangliosides in nerve sprouting and cell contact formation: an ultracytochemical in vitro study. | in cichlid fish (oreochromis mossambicus) primary cell cultures from whole brain and optic tectum, the differentiation-dependent distribution of polysialogangliosides on the outer cell surface has been followed on an ultrastructural level. for this, a two-step labeling technique with the monoclonal mouse antibody q211, recognizing a polysialoganglioside-associated epitope, followed by a secondary igm antibody, coupled to colloidal gold sols as an electron-dense marker, has been used. the gold gr ... | 1989 | 2723652 |
| basolateral inositol transport by intestines of carnivorous and herbivorous teleosts. | myoinositol transport by isolated basolateral membrane vesicles was characterized from intestines of the herbivorous tilapia (oreochromis mossambicus) and the carnivorous eel (anguilla anguilla). [3h]myoinositol transport occurred by nonelectrogenic, facilitated diffusion independent of cation gradients and was inhibited by phloretin (ki = 0.6 and 0.9 mm for tilapia and eel, respectively) but not by phloridzin. kinetic analysis of myoinositol influx disclosed no differences in concentration yiel ... | 1989 | 2923212 |
| brush-border inositol transport by intestines of carnivorous and herbivorous teleosts. | transport characteristics of myoinositol by isolated brush-border membrane vesicles of two fish, the herbivorous tilapia (oreochromis mossambicus) and the carnivorous eel (anguilla anguilla), were measured. [3h]myoinositol uptake by vesicles of both fish was stimulated by a transmembrane na gradient, was electrogenic, and was inhibited by phloridzin. kinetic analysis of myoinositol influx disclosed species differences (tilapia, k = 0.15 mm, jmax = 0.2 nmol.mg protein-1.min-1; eel, k = 2.6 mm, jm ... | 1989 | 2923211 |
| hepatic 5'-monodeiodinase activity in teleosts in vitro: a survey of thirty-three species. | the in vitro hepatic 5'-monodeiodination of thyroxine (t4) to triiodothyronine (t3) in oreochromis mossambicus, channa striata, clarias batrachus, cyprinus carpio and oxyeleotris marmorata was found to be time, ph and temperature dependent, and related to the amount of substrate (t4) and homogenate introduced into the reaction vessel, in a manner which was consistent with menton-michaelis kinetics, and thus indicative of an enzyme-regulated process. dithiothreitol introduced into the reaction ve ... | 1990 | 24221892 |
| prolactin receptors in liver, kidney, and gill of the tilapia (oreochromis mossambicus): characterization and effect of salinity on specific binding of iodinated ovine prolactin. | specific binding of 125i-ovine prolactin (oprl) to microsomal fractions from gill, kidney, and liver of adult tilapia was determined. specific binding varied among tissues, the highest values being displayed by kidney membranes. in the liver, the binding of oprl was not strongly displaced by tilapia prolactins (tprl177 and tprl188), although tprl177 was six times more potent than tprl188. on the other hand, in kidney and gill membranes, the two tprls were equipotent. tilapia prls showed low pote ... | 1990 | 2338217 |
| effect of hypophysectomy, replacement therapy with ovine prolactin, and cortisol and triiodothyronine treatment on prolactin receptors of the tilapia (oreochromis mossambicus). | the effects of hypophysectomy and subsequent replacement therapy with ovine prolactin (oprl) on specific binding of 125i-oprl to gill, kidney, and liver membranes of male tilapia were examined. the possible control of prolactin receptors by cortisol (f) and triiodothyronine (t3) was also studied using intact animals. in gill and kidney, hypophysectomy resulted in a significant decrease in specific binding that was partially restored (threefold increase) by three injections of oprl, suggesting a ... | 1990 | 2338218 |
| some unusual features of mycobacteriosis in the cichlid fish oreochromis mossambicus. | adult, aquarium-reared oreochromis mossambicus (tilapia mossambica), which were naturally infected with mycobacterium marinum, displayed non-healing skin ulcers and other clinical signs considered to be typical of piscine mycobacteriosis. however, in addition, they frequently had melanotic foci in the skin and spleen, due to the presence of pigment cells surrounding the cutaneous and splenic inflammation. such melanotic foci have never been reported in response to mycobacteriosis. all fish also ... | 1990 | 2365849 |
| metabolic effects of bovine growth hormone in the tilapia oreochromis mossambicus. | 1. bovine growth hormone (bgh) was injected into tilapia intramuscularly at a dose of 50 micrograms/100 g/day for a total of five injections. control fish received saline instead. 2. the serum concentrations of amino acid and glucose were significantly higher and hepatic glycogen concentration and glycogen synthetase activity significantly lower in the bgh-treated fish than those in the control fish. 3. the serum concentrations of protein, lipid and cholesterol, and the hepatic concentrations of ... | 1991 | 1679700 |
| residues of polychlorinated biphenyls (pcbs) in fish, water and sediment from shing mun river. | the level and pattern of contamination by polychlorinated biphenyls (pcbs) were investigated in tilapia, oreochromis mossambicus (peters), sediment, and water from the shing mun river. the range of total pcbs was 12.9 ng/g to 181.6 ng/g wet weight in tilapia, 12.7 ng/g to 46.0 ng/g freeze-dried weight in sediment, and 3.8 ng/l to 13.6 ng/l in water. the effect of biomagnification was also observed, pcb concentrations increased from water to sediment to tilapia. pcb congeners occurred in such a w ... | 1991 | 1781935 |
| laboratory evaluation of b-2 as a molluscicide in the control of the snail intermediate hosts of schistosomiasis in south africa. | there is a continuous need for the development and evaluation of new, inexpensive but highly effective molluscides for the control of freshwater snails acting as intermediate hosts of schistomiasis. for this reason b-2 (hokko chemical industry co. ltd, japan), also called phebrol (sodium 2,5-dichloro-4-bromophenol), was evaluated in our laboratory as a candidate molluscicide for the control of freshwater snails in south africa. bulinus africanus and biomphalaria pfeifferi, intermediate hosts of ... | 1991 | 1796888 |
| insulin-like growth factor i-like immunoreactivity in serum and tissues of the tilapia, oreochromis mossambicus. | tilapia serum was acidified with 0.5 m hcl and then chromatographed on an octadecasily-silica column. after washing with 4% acetic acid, the column was eluted with methanol. the eluate was evaporated to dryness. the sample cross-reacted in a human insulin-like growth factor i (igf-i) radioimmunoassay, suggesting immunochemical similarity to human igf-i. igf-i-like immunoreactivity was present at high levels in tilapia liver. other tissues containing igf-i-like immunoreactivity included the gonad ... | 1991 | 1930252 |
| amino acid sequence of growth hormone isolated from medium of incubated pituitary glands of tilapia (oreochromis mossambicus). | the amino acid sequence of tilapia (oreochromis mossambicus) growth hormone (gh) was determined directly by edman degradation of peptide fragments generated by lysyl endopeptidase and trypsin digestion. the n-terminal residue was deduced to be pyroglutamic acid through the use of pyroglutamyl aminopeptidase; its removal allowed amino acid sequence determination of the remainder of the n-terminal trypsin peptide by edman degradation. tilapia gh is composed of 187 amino acid residues and shows hig ... | 1991 | 2019405 |
| the molluscicidal properties of apodytes dimidiata subsp. dimidiata (icacinaceae). | the molluscicidal and piscicidal activity of extracts from the leaves, berries and bark of the tree apodytes dimidiata were evaluated experimentally. the leaves were highly toxic to both bulinus africanus and biomphalaria pfeifferi, the intermediate host snails of schistosoma spp. b. pfeifferi profusely exuded mucus which appeared to render some protection against the toxic substance(s) of the plant but did not prevent their eventual demise. the lc10 after one-hour exposures of fish (oreochromis ... | 1991 | 2051521 |
| effect of thyroid hormones on hatching in the tilapia, oreochromis mossambicus. | incubation of fertilized eggs of oreochromis mossambicus in media containing varying doses of t3, t4, and phenylthiocarbamide (ptc) indicated that the thyroid hormones delayed hatching, while the antithyroid drug accelerated it. denuded eggs were also incubated in the above media and the media were assayed at various time intervals for hatching enzyme. the thyroid hormones delayed hatching enzyme release, while ptc enhanced it. the possible role of thyroid hormones in the control of the hatching ... | 1991 | 2055445 |
| morphogenetic development of the area octavolateralis in the cichlid fish oreochromis mossambicus. | in the cichlid fish oreochromis mossambicus the area octavolateralis in the brain stem was studied using histological techniques and labelling of afferents from the inner ear and the lateral line system with horseradish peroxidase (hrp). adult fish and fish larvae aged 2, 4, 7, 10, and 15 days post hatching were investigated in order to analyze the ontogenetic development of these sensory systems during the critical phase of maturation from relatively immobile stages to actively swimming larvae. ... | 1991 | 1802932 |
| neurotensin, substance p, gastrin/cholecystokinin, and bombesin in the intestine of the tilapia (oreochromis mossambicus) and the goldfish (carassius auratus): immunochemical detection and effects on electrophysiological characteristics. | the distribution of neurotensin-, substance p-, gastrin/cholecystokinin/carerulein- and bombesin-like immunoreactivities has been studied in the gut of the tilapia (oreochromis mossambicus) and the goldfish (carassius auratus) using immunohistochemistry and radioimmunoassay; the electrophysiological effects of these peptides on the intestinal epithelium were also examined with the ussing-type chamber technique. neurotensin- and gastrin/cholecystokinin/caerulein-like immunoreactivities were prese ... | 1992 | 1283377 |
| fishes of district sundargarh, orissa, with special reference to their potential in mosquito control. | an extensive fish fauna survey was carried out in sundargarh, a malaria-endemic district in orissa, during 1988 to 1990 to identify and evaluate the indigenous larvivorous fishes for mosquito control. in all, 57 species belonging to 19 families under 6 orders were found in the local water bodies. on laboratory evaluation against anopheline and culicine larvae, six potential larvivorous fishes, viz. aplocheilus panchax, oryzias melastigma, oreochromis mossambicus, gambusia affinis, danio (b.) rer ... | 1992 | 1363317 |
| identification of insulin-like growth factor-binding proteins in the circulation of four teleost fish species. | insulin-like growth factor-binding proteins (igf-bps) were demonstrated in the circulation of four teleost fish species. in the coho salmon (oncorhynchus kisutch), serum binding of 125i-labelled human igf-i (125i-higf-i) was competitively inhibited by addition of excess recombinant bovine igf-i (rbigf-i) in a manner similar to that when rat serum was used. western-ligand blot procedure using the same labelled hormone identified at least three major forms of igf-bps in the plasma of all four tele ... | 1992 | 1380067 |
| growth hormone binding sites in tilapia (oreochromis mossambicus) liver. | 125i-labeled bovine and tilapia growth hormones were used to assess the presence of growth hormone receptors in membranes prepared from tissues of the tilapia oreochromis mossambicus. the highest level of specific binding was detected in liver membranes from animals of both sexes and the binding was protein-dependent. tilapia growth hormone, bovine growth hormone, and ovine prolactin, but not tilapia prolactin, potently inhibited the hepatic binding of 125i-labeled bovine growth hormone. scatcha ... | 1992 | 1505720 |
| patterns of thyroxine and triiodothyronine in serum and follicle-bound oocytes of the tilapia, oreochromis mossambicus, during oogenesis. | this study describes simultaneous measurements of thyroid hormones, thyroxine (t4) and triiodothyronine (t3), in the oocytes and serum of a female teleost fish over a complete reproductive cycle. we have identified patterns in circulating t4 and t3 levels as well as their accumulation into oocytes during the reproductive cycle of the tilapia (oreochromis mossambicus). this is the first description of the patterns with which thyroid hormones accumulate in teleost oocytes. the sampling strategy us ... | 1992 | 1577243 |
| cortisol content of eggs and larvae of teleosts. | the whole-animal content of the cortisol was measured in embryos and larvae of tilapia (oreochromis mossambicus), rainbow trout (oncorhynchus mykiss), ayu (plecoglossus altivelis), milkfish (chanos chanos), and yellowfin bream (acanthropagrus latus) by radioimmunoassay following the validation of an extraction method. the total cortisol content in tilapia was 50.3 +/- 19.1 pg immediately following fertilization, then decreased abruptly and maintained a lower level of 10-17 pg until larval hatchi ... | 1992 | 1601267 |
| effects of acclimation to hypertonic environment on plasma and pituitary levels of two prolactins and growth hormone in two species of tilapia, oreochromis mossambicus and oreochromis niloticus. | specific radioimmunoassays (rias) for the pair of tilapia prolactins (tprls) and growth hormone (tgh) were developed using antisera raised in rabbits. anti-tprl177 did not cross-react with tprl188 and tgh. anti-tprl188 did not cross-react with tprl177 and showed slight cross-reaction (3.1%) with tgh. anti-tgh showed negligible cross-reactions with tprl177 (0.4%) and tprl188 (1.6%). pituitary homogenates and plasma from oreochromis niloticus exhibited displacement curves parallel to the standards ... | 1993 | 8428646 |
| brain creatine kinase activity during ontogeny of the cichlid fish oreochromis mossambicus and the clawed toad xenopus laevis, influence of gravity? | the development of creatine kinase (ck) activity was studied in the brain of cichlid fish and clawed toads. the activity of ck in the whole brain of the fish decreases immediately after hatching (stage 6) from values of about 135 nmol substrate cleaved/mg protein/min to a value of about 105 at stage 8 (5 days post hatch at 20 degrees c). with the exception of a significant peak (125 nmol) between stages 9 and 10 (7 and 9 days respectively, post hatch at 20 degrees c) and a small intermediate pea ... | 1993 | 8457775 |
| immunocytochemical and immunogold localization of two prolactin isoforms in the same pituitary cells and in the same granules in the tilapia (oreochromis mossambicus). | the tilapia pituitary secretes two forms of prolactin (tprl) and a single growth hormone (tgh). the tprls share only 69% sequence identity and are designated tprl177 and tprl188 to indicate the number of amino acid residues in each isoform. our aim was to develop specific antisera for detection of these three related polypeptides. ten peptides corresponding to unique epitopes on the tprls and two peptides of tgh were synthesized using solid-phase methods, conjugated to carrier proteins, and used ... | 1993 | 7679085 |
| adrenocorticotropic hormone in relation to interrenal function during stress in tilapia (oreochromis mossambicus). | this study examines acth-like immunoreactivity in the pituitary pars distalis and pars intermedia of the freshwater teleost oreochromis mossambicus (tilapia). two products (tactha and tacthb) were present in both lobes. these two products also accounted for the majority of the acth i.r. when in vitro pars distalis incubation medium was analyzed by hplc. in a homologous bioassay the two tilapia acth-like molecules and human acth1-39 possessed similar corticotropic potency. the peptides were quant ... | 1994 | 7883141 |
| parasites of south african freshwater fish. vi. nematode parasites of some fish species in the kruger national park. | the nematode parasites of 30 spot-tailed robbers, brycinus imberi, five tiger-fish, hydrocynus vittatus, 77 large-scaled yellowfish. barbus marequensis, two mudsuckers, labeo molybdinus, 114 catfish, clarias gariepinus, 46 silver barbel, schilbe intermedius, 66 squeakers, synodontis zambezensis, three eels, anguilla spp., 83 mozambique bream, oreochromis mossambicus, 81 red-breasted bream, tilapia rendalli swierstrae and 32 large-mouthed bream, serranochromis meridianus, caught in the sabie, cro ... | 1994 | 7898896 |
| diel variations in antibody response to bovine serum albumin in oreochromis mossambicus (peters). | influence of time of antigen administration on antibody response to bovine serum albumin in the fish, o. mossambicus and variations in serum antibody titre during a 24 hr cycle are reported. significant variations were observed in magnitude of antibody response in groups of fishes immunized at different times of the day. magnitude of the response was maximal in fish immunized at 16 hr and minimal in those immunized at 4 hr. serum antibody titres in a 24 hr study also varied markedly with general ... | 1994 | 7959923 |
| parasites of south african freshwater fish. vii. nematodes of some scaled fishes from the hartbeespoort dam, transvaal. | the nematode parasites of 16 large-scaled yellowfish, barbus marequensis, six silverfish, barbus mattozi, six small-scaled yellowfish, barbus polylepis, 52 canary kurper, chetia flaviventris, 11 carp, cyprinus carpio, 45 mozambique bream, oreochromis mossambicus and a single-banded bream, tilapia sparrmani, caught in the hartbeespoort dam, transvaal, were collected, identified and counted. contracaecum spp. larvae were recovered from one o. mossambicus, 40 chetia flaviventris, three cyprinus car ... | 1994 | 7596571 |
| behavioural and biochemical investigations of the influence of altered gravity on the cns of aquatic vertebrates during ontogeny. | quantitative data are presented on the influences of hyper-gravity (3 +/- 1g) and of simulated weightlessness (approximately 0g) during early ontogeny of cichlid fish (oreochromis mossambicus) and clawed toad (xenopus laevis, daudin) demonstrating changes in the swimming behaviour and the brain energy and plasma membrane metabolism. after return to 1g conditions, hyper-g reared fish and toads express the well known "loop-swimming" behaviour. by means of a computer based video analyzing system ... | 1994 | 11537932 |
| effects of cadmium, naphthalene, and ddvp on gut carbohydrases activity in bream (abramis brama l.) and mozambique tilapia (oreochromis mossambicus peters). | 1994 | 8142701 | |
| bacterial lipopolysaccharide (lps) and interleukin 1 (il-1) exert multiple physiological effects in the tilapia oreochromis mossambicus (teleostei). | to gain insight in immuno-endocrine communication in teleosts the physiological effects of interleukin 1 and bacterial lipopolysaccharide in teleosts were investigated. tilapia (oreochromis mossambicus) were treated with murine interleukin 1 and e. coli lipopolysaccharide in vivo, and lipopolysaccharide was administered to pituitary lobes and head kidneys in vitro. the integument of the fish appeared to be a sensitive target for the preparations tested, since proliferation of chloride cells and ... | 1995 | 7622674 |
| endorphin and msh in concert form the corticotropic principle released by tilapia (oreochromis mossambicus; teleostei) melanotropes. | hplc characterization of tilapia pituitary endorphins using an antibody specific for n-terminally acetylated endorphins yielded three major peaks in the neurointermediate lobe, but none in the pars distalis. the melanotropes secreted two of the immunoreactive products in vitro, one of which coeluted with xenopus laevis n-ac-beta-end(1-8). this immunoreactive fraction also coeluted with diacetyl-alpha-msh. evidence is presented that the noteworthy corticotropic potency of this hplc fraction, prev ... | 1995 | 7651900 |
| an aquatic toxicological evaluation of fenthion in the context of finch control in south africa. | queletox, containing fenthion as active ingredient, is the avicide formulation used in south africa to control red-billed finches (quelea quelea). control measures involve night spraying of roosting areas with a light aircraft. since roosting areas often include reedbeds along riversides and on islands, proper control is difficult without exposing the aquatic environment to some risk of contamination. this study tested the acute effects of fenthion, in association with the queletox formulation, ... | 1995 | 8521783 |
| modulation of humoral immune response by tannery effluent in oreochromis mossambicus (peters). | fish were treated with various sublethal concentrations of tannery effluent. a concentration-dependent suppression of both primary and secondary antibody responses to bovine serum albumin (bsa) was observed after primary or secondary immunization. the fish were able to compensate the effluent-induced suppression of antibody response when the antigen (bsa) was administered along with an oil adjuvant. | 1995 | 8575812 |
| neobenedenia girellae (hargis, 1955) yamaguti, 1963 (monogenea: capsalidae) from cultured marine fishes of japan. | the monogenean neobenedenia girellae (hargis, 1955) yamaguti, 1963 is redescribed and reported for the first time in japan. the parasite was recovered from the body surface, fins, and occasionally from the eyes of 14 species, comprising 5 families of cultured marine fishes from several localities in southwestern japan. neobenedenia melleni (maccallum, 1927) sensu kaneko et al. (1988) from tilapia (oreochromis mossambicus) in hawaii is synonymized with this species. examination of original specim ... | 1995 | 7707197 |
| cloning and sequence analysis of a hypothalamic cdna encoding a d1c dopamine receptor in tilapia. | physiological and pharmacological studies have indicated that during acid stress a d1-like dopamine receptor becomes functional on intermediate pituitary melanocyte-stimulating hormone cells of tilapia (oreochromis mossambicus). as a first step towards physiological expression studies we isolated a d1-like dopamine receptor from a tilapia hypothalamus cdna library. construction of a phylogenetic tree of most of the d1-like receptors known in human, rat, xenopus, goldfish and drosophila revealed ... | 1996 | 8765745 |
| gonadotropin-releasing hormone gene expression in teleosts. | expression of multiple molecular forms of gonadotropin-releasing hormone (gnrh) mrnas and gnrh peptides were examined in the brains of tilapia (oreochromis mossambicus) and sockeye salmon (oncorhynchus nerka), using in situ hybridization histochemistry and immunohistochemical techniques. after otherwise identical conditions, lesser background and stronger gnrh hybridization signals were observed on cryostat vs. paraffin sections. in both fresh and bouin's-fixed paraffin-embedded tissues, there w ... | 1996 | 8883955 |
| in situ hybridization for two differentially expressed gnrh genes following estrogen and triiodothyronine treatment in the brains of juvenile tilapia (cichlid). | using in situ hybridization, messenger rnas for gonadotropin-releasing hormone (gnrh) were seen distributed differently in the brains of 40-day-old juvenile tilapia oreochromis mossambicus. while salmon-gnrh mrna was localized in the nucleus olfactoretinalis (terminal nerve ganglia), chicken ii-gnrh mrna mrna was observed in the midbrain nucleus. various concentrations (0.1-10 mg) of estradiol benzoate and triiodothyronine, given over a 24 h period, had no effects on mrna levels of salmon- and c ... | 1996 | 8945746 |
| the effects of cyanobacteria and the cyanobacterial toxin microcystin-lr on ca2+ transport and na+/k+-atpase in tilapia gills | the effects of cytotoxic substances from cyanobacteria on ionic transport processes in tilapia (oreochromis mossambicus) were examined. inhibitory effects on ionic transport including whole-body ca2+ fluxes and p-type atpases of the gill were found. the compounds tested were (1) purified microcystin-lr (mc-lr), a heptapeptide hepatotoxin produced by the cyanobacterium microcystis aeruginosa, (2) extracts from m. aeruginosa strain pcc 7820, a strain producing mc-lr and other microcystin variants, ... | 1996 | 9319198 |
| plasma and urine levels of c18, c19 and c21 steroids in an asynchronous fish, the tilapia oreochromis mossambicus (teleostei, cichlidae). | female and male tilapia, oreochromis mossambicus, were treated with luteinizing hormone-releasing hormone analogue (lh-rha) and pimozide (pim) or with human chorionic gonadotropin (hcg) to stimulate gonadal development and sexual maturation. plasma (both sexes) and urine (males) samples were collected periodically for steroid analysis by radioimmunoassay. plasma levels of estradiol-17 beta (3-6 ng/ml) and testosterone, higher in female (up to 25 ng/ml) than in male (6-13 ng/ml; p < 0.05), were i ... | 1996 | 9375364 |
| early development in aquatic vertebrates in near weightlessness during the d-2 mission statex project. | aboard the german-spacelab-mission d-2 the project "gravity perception and neuronal plasticity (statex ii)" was performed. statex is for statolith experiment. objects were growing tadpoles of the south african toad (xenopus laevis d.) and a juvenile cichlid fish (oreochromis mossambicus). the results give a broader base for the understanding of how environmental stimuli (e.g. linear accelerations) affect the development and function of the gravity perceiving systems in these two vertebrates. ... | 1996 | 11538629 |
| effects of fasting, medium glucose, and amino acid concentrations on prolactin and growth hormone release, in vitro, from the pituitary of the tilapia oreochromis mossambicus. | previous investigations have shown that the release of prl and gh from the tilapia pituitary is directly sensitive to osmotic pressure and a variety of endocrine and neuroendocrine factors. the present studies were aimed at determining whether the spontaneous release of prl and gh, in vitro, is: (1) sensitive to the nutritional status of the fish, and (2) responsive to variations in the d-glucose and total amino acid content of the incubation medium. in the first series of experiments, male fish ... | 1996 | 1398000 |
| a comparative analysis of somatolactin-related immunoreactivity in the pituitaries of four neopterygian fishes and one chondrostean fish: an immunohistochemical study. | an antiserum to cod somatolactin (sl) was used for immunohistochemical screening for the pars intermedia of two teleosts (oreochromis mossambicus and gymothorax meleagris), two holostean fishes (lepisosteus osseus and amia calva), and a chondrostean fish (acipenser fulvescens) for sl-immunopositive (sl-ir) cells. as expected, a subset of the epithelial cells in the pars intermedia of o. mossambicus (tilapia) was immunopositive for sl, and the remainder of the epithelial cells was immunopositive ... | 1996 | 8860312 |
| exocytotic release of vasoative intestinal polypeptide and serotonin from mucosal nerve fibres and endocrine cells of the intestine of the goldfish (carassius auratus) and the tilapia (oreochromis mossambicus): an ultrastructural study. | in earlier studies were determined the effect, presence and ultrastructure of vasoactive intestinal polypeptide (vip) and 5-hydroxytryptamine (5-ht)-containing nerve fibres in the tilapia and goldfish intestinal mucosa. 5-ht-labelled varicosities were found close to the epithelial cells; however, synaptic membrane specializations have never been observed. vip-like immunoreactive nerve fibres appear to be located less frequently close to the goldfish epithelium, as in the tilapia intestine, in wh ... | 1997 | 9088944 |
| partial cloning of the hormone-binding domain of the cortisol receptor in tilapia, oreochromis mossambicus, and changes in the mrna level during embryonic development. | cortisol is one of the central hormones in osmoregulation in fish, especially in seawater adaptation. a cdna of 453 bp was cloned from liver mrna of freshwater-reared tilapia (oreochromis mossambicus), by reverse transcription polymerase chain reaction (rt-pcr) with primers designed for the hormone-binding domain of glucocorticoid receptors (grs) in mammals and rainbow trout. the sequence of pcr product has 83% homology to the trout gr at the nucleotide level and 92% at the amino acid level. the ... | 1997 | 9378267 |
| evidence that gonadotropin-releasing hormone (gnrh) functions as a prolactin-releasing factor in a teleost fish (oreochromis mossambicus) and primary structures for three native gnrh molecules. | three forms of gonadotropin-releasing hormone (gnrh) are isolated and identified here by chemical sequence analysis for one species of tilapia, oreochromis niloticus, and by hplc elution position for a second species of tilapia, o. mossambicus. of the three gnrh forms in o. mossambicus, chicken gnrh-ii (cgnrh-ii) and sea bream gnrh (sbgnrh) are present in greater abundance in the brain and pituitary than salmon gnrh (sgnrh). these three native forms of gnrh are shown to stimulate the release of ... | 1997 | 9390014 |
| acute toxicity evaluation of the plant molluscicide, apodytes dimidiata (icacinaceae), to eisenia fetida (oligochaeta) and oreochromis mossambicus (cichlidae) in south africa. | the crude aqueous leaf extract of a south african tree, apodytes dimidiata, has been identified as a potential molluscicide for snail control in anti-schistosomiasis programmes in rural communities. before preliminary field trials could be implemented its acute toxic effects on two non-target organisms were examined in accordance with the oecd guidelines to identify any potential hazards that might arise from the plant's use. laboratory results showed that working molluscicidal concentrations of ... | 1997 | 9083581 |
| hypothalamic regulation of the pituitary-thyroid axis in the tilapia oreochromis mossambicus. | electrolytic lesioning of the preoptic area resulted in an increase in plasma thyroxine (t4) and reverse triiodothyronine (rt3) 10 days later; plasma triiodothyronine (t3) levels were not affected, so that there was also a significant decrease in the t3:t4, but not rt3:t4, ratios. no significant changes in t4, t3, or rt3 levels were observed in fish with lesions in either the anterior or posterior portions of the lateral tuberal nucleus. the pituitary contents of growth hormone and the two prola ... | 1997 | 9126467 |
| insulin-like growth factor i in the teleost oreochromis mossambicus, the tilapia: gene sequence, tissue expression, and cellular localization. | using reverse transcription-pcr and molecular cloning, the complementary dna sequence encoding preproinsulin-like growth factor i (igf-i) of a teleost, the tilapia (oreochromis mossambicus) was established from liver. at the amino acid level, tilapia igf-i shows all residues necessary for the maintenance of tertiary structure and shares about 80% identity with igf-i from other teleosts. the b and a domains of tilapia igf-i show more than 90% homology to those of other teleosts and 86-93% to thos ... | 1997 | 9275043 |
| fatty acids from the cyanobacterium microcystis aeruginosa with potent inhibitory effects on fish gill na+/k+-atpase activity. | fatty acids from two strains of the cyanobacterium microcystis aeruginosa, pcc 7820 (a strain that produces the hepatotoxin microcystin-lr, mc-lr) and cya 43 (a strain that produces only small quantities of mc-lr), were extracted, partially characterised and tested for their inhibitory effect on the k+-dependent p-nitrophenol phosphatase (pnppase) activity of tilapia (oreochromis mossambicus) gill basolateral membrane. thin-layer chromatography of the lipids from dichloromethane:methanol extract ... | 1998 | 9390939 |
| effect of edta on reduction of copper toxicity in oreochromis mossambicus (peters). | 1998 | 9528709 | |
| isolation and characterization of tilapia (oreochromis mossambicus) insulin-like growth factors gene and proximal promoter region. | to understand the molecular mechanism which controls the transcription of the insulin-like growth factors (igfs) gene, we have cloned and sequenced the cdna for the proximal promoter region of the tilapia igfs gene and have characterized its activity by chloramphenicol acetyltransferase (cat) transient transfected expression assays. tilapia (oreochromis mossambicus) igf-i cdna (549 bp) was amplified by pcr from single-stranded cdna of growth hormone (gh)-induced liver rna using a pair of oligonu ... | 1998 | 9570153 |
| 3,5,3'-triiodothyronine (t3) clearance and t3-glucuronide (t3g) appearance kinetics in plasma of freshwater-reared male tilapia, oreochromis mossambicus. | distribution and metabolism of the thyroid hormone 3,5, 3'-l-triiodothyronine (t3) were studied in several ways to gain insights into these processes in the warm water fish tilapia oreochromis mossambicus. trace doses of 125i-labeled t3 (t*3)1 were injected intraarterially, extraarterially, or intraperitoneally in freshwater-reared male tilapia to explore plasma clearance kinetic responses to these different input modalities. multicompartmental analysis of the plasma clearance data indicated a k ... | 1998 | 9679085 |
| isoform expression of na+-k+-atpase alpha-subunit in gills of the teleost oreochromis mossambicus. | three isoform-specific antibodies, 6f against the alpha1-isoform of the avian sodium pump, hered against the rat alpha2-isoform, and ax2 against the rat alpha3-isoform, were used to detect the expression of na+-k+-atpase alpha-subunits in gills of a teleost, the tilapia (oreochromis mossambicus). tilapia gill tissue showed positive reactions to antibodies specific for alpha1- and alpha3-isoforms. the results of immunoblots were converted to numerical values (relative intensities) by image analys ... | 1998 | 9728093 |
| pentastomid infections in cichlid fishes in the kruger national park and the description of the infective larva of subtriquetra rileyi n. sp. | during 1995, studies were conducted on the pentastome fauna of the cichlid fishes tilapia rendalli and oreochromis mossambicus in the kruger national park. the prevalence of infective pentastome larvae was 40.5% in t. rendalli and 9.2% in o. mossambicus. encapsulated nymphs of leiperia cincinnalis were taken from the mesentery, while sebekia wedli was either encapsulated or free-living in the swim bladder. the subtriquetrids moved about freely in the swim bladder. l. cincinnalis was present in 0 ... | 1998 | 9809320 |
| experimental studies on the life-cycle of sebekia wedli (pentastomida: sebekidae). | four young nile crocodiles (crocodylus niloticus) were infected with infective pentastome larvae obtained from naturally infected mozambique bream, oreochromis mossambicus, and red-breasted bream, tilapia rendalli swierstrai in the kruger national park. at day 95 post infection one of the crocodiles died and three female and four male s. wedli were recovered from its lungs. one pair was found in copula but the uteri of the females were not yet developed. males and females were of about equal siz ... | 1998 | 10192834 |
| modulation of humoral immune response by ascorbic acid in oreochromis mossambicus (peters). | fish were treated with sublethal doses of ascorbic acid before being immunised with different physical forms of bovine serum albumin (bsa). ascorbic acid elevates the antibody response to all the physical forms of bsa tested. generally, there was an inverse relationship between the dose of ascorbic acid and antibody response. in fish administered with multiple doses of ascorbic acid, no significant enhancement of antibody response was observed. | 1998 | 10356967 |
| distribution and feeding efficacy of larvivorous fishes of goa. | fifteen species of larvivorous fishes, viz. aplocheilus blockii, aplocheilus lineatus, rasbora daniconius, puntius vittatus, puntius melanampyx pradhani, puntius amphibius, macropodus cupanus, danio aequipinnatus. oreochromis mossambicus, heteropneustes sp, therapon jarbua, etroplus suratensis, mugil cephalus. osteochilichthys nashii and hypselobarbus dobsoni were collected from different parts of goa. their habitat wise distribution was studied and density was recorded. daily average consumptio ... | 1998 | 10497843 |
| sodium and calcium balance in mozambique tilapia, oreochromis mossambicus, raised at different salinities. | mozambique tilapia, oreochromis mossambicus, born and raised in five salinities, viz. (relatively soft) fresh water, 25, 50, 75% and full-strength sea-water, were analyzed for ionoregulatory performance (in particular sodium and calcium handling) and growth. this tilapia regulates its blood serum mineral composition rather effectively; however, in sea-water serum concentrations of sodium, chloride and calcium (in males only) were increased, as was the serum osmolarity. in sea-water, the total bo ... | 1998 | 11248987 |
| genomic organization of the oreochromis mossambicus glutamate receptor subunit 2 beta gene (fglur2 beta): presence of two different 5'-untranslated regions. | the ampa (alpha-amino-3-hydroxy-5-methyl-isoxazole-4-propionic acid)-preferring receptor is one of the pharmacologically defined ionotropic glutamate receptors, which mediate fast excitatory synaptic transmission in the central nervous system of vertebrates. here, we report the mapping of the transcriptional start points and identification of the intron-exon boundaries of the teleost ampa receptor subunit gene fglur2 beta. fglur2 beta and the mouse glur2 share a similar genomic organization, hav ... | 1999 | 10524256 |
| a line2 repetitive dna sequence from the cichlid fish, oreochromis niloticus: sequence analysis and chromosomal distribution. | we report the cloning and characterization of a long interspersed nucleotide element (line) from a cichlid fish, oreochromis niloticus, and show the distribution of this element, called ciline2 for cichlid line2, in the chromosomes of this species. the identification of an open reading frame in ciline2 with amino acid sequence similarity to reverse transcriptases encoded by line-like elements in caenorhabditis elegans, platemys spixii, schistosoma mansoni, gallus gallus (cri), drosophila melanog ... | 1999 | 10654084 |
| structure and functional analysis of a tilapia (oreochromis mossambicus) growth hormone gene: activation and repression by pituitary transcription factor pit-1. | a gene encoding the tilapia mossambica (oreochromis mossambicus) growth hormone (tigh) was isolated and sequenced. the gene spans 5.6 kb, including 3.7 kb of 5' and 0.2 kb of 3' flanking sequences and a 1.7-kb transcription unit comprised of six exons and five introns. the gene and the 5' flanking region contain several potential binding sites for pit-1, a key transcription activator of mammalian gh genes. one of these (-57/-42) is highly conserved in fish gh genes. it activates transcription in ... | 1999 | 10390158 |
| biochemical and histopathological changes in certain tissues of oreochromis mossambicus (trewaves) under ambient urea stress. | 1999 | 10423492 | |
| molecular cloning of clc chloride channels in oreochromis mossambicus and their functional complementation of yeast clc gene mutant. | we have cloned two members of the clc chloride channel family (omclc-3 and omclc-5) from gill cdna libraries of the euryhaline tilapia oreochromis mosammbicus. at the amino acid level, omclc-3 is 90.5% identical to rat clc-3 and omclc-5 is 79.2% identical to rat clc-5. ribonuclease protection assay revealed that omclc-5 was mainly expressed in the gill, kidney, and intestine in both freshwater- (fw) and seawater- (sw) adapted tilapia. although the mrna of omclc-3 was broadly expressed in tissues ... | 1999 | 10082675 |
| on the influence of altered gravity on the growth of fish inner ear otoliths. | inner ear stones (otoliths) of developing cichlid fish (oreochromis mossambicus) were marked with the calcium tracer alizarin-complexone (ac) at 1g-earth gravity before and after a longterm (20 days) stay of the animals at moderate hypergravity conditions (3g; centrifuge). ac deposition at the otoliths resulted in two fluorescence bands, which enclosed the area grown during exposure to altered gravity. this area was measured with regard to size and asymmetry (size difference between the left and ... | 1999 | 11542523 |
| direct electrospray-ionization mass spectrometric analysis of the major ganglioside from crucian carp liver after thin layer chromatography. | ganglioside patterns from crucian carp brain, muscle, and liver as well as liver gangliosides of roach, carp, the cichlid oreochromis mossambicus, pigeon, dwarf hamster, and calf were comparatively analyzed by high performance thin layer chromatography (hptlc). to achieve a rapid estimation on potentially interesting ganglioside compounds, electrospray-ionization mass spectrometry (ms) was directly applied to a chloroform/methanol extract of the major tlc band of crucian carp liver. the spectrum ... | 1999 | 10327597 |
| systemic zygomycosis in farmed tilapia fish. | decreased feed intake and persistent low-level mortality in a production tank of hybrid tilapia (oreochromis niloticus x oreochromis mossambicus x oreochromis aureus) prompted the submission of three affected fish for diagnosis. consistent macrosopical findings included multifocal dermal haemorrhage, excess abdominal fluid and an enlarged friable liver. on microscopical examination, broad non-septate fungal hyphae and chlamydospores were identified within numerous internal organs, often within a ... | 1999 | 10486168 |
| [rodlet cells of fish in calcium deficiency in the external environment]. | the ultrastructure of rodlet cells from the heat kidney of the carp cyprinus carpio l. and tilapia oreochromis mossambicus peters has been studied. all the investigated cells displayed a common structure. the number of rodlet cells increased after exposure of fishes in ca(2+)-free water or in the water supplemented with nh4+ or cd2+. the latter were found to suppress ca(2+)-absorption from the environment. | 2000 | 11204648 |
| cloning and expression analysis of an inducible hsp70 gene from tilapia fish. | we isolated and characterized the tilapia (oreochromis mossambicus) hsp70 gene, highly homologous to other hsp70 genes. a dramatic increase of tilapia hsp70 mrna levels was observed after heat shock of whole animals in all organs tested. reporter constructs were tested for transient expression in carp cells and in microinjected zebrafish embryos. the entire isolated regulatory region (-851/+157) was able to mediate heat shock inducible expression of the reporter gene, with no preference for a pa ... | 2000 | 10828441 |
| purification, characterization, and bioassay of prolactin and growth hormone from temperate basses, genus morone. | prolactin (prl) and two variants of growth hormone (gh), purified from pituitaries of striped bass (morone saxatilis) and its hybrid with white bass (m. saxatilis x m. chrysops) by gel filtration chromatography under alkaline conditions followed by reversed-phase high pressure liquid chromatography, appear similar between species. both the minor (gh i) and the major (gh ii) forms of purified gh appeared as single bands (m(r) approximately 23,000) after sodium dodecyl sulfate-polyacrylamide gel e ... | 2000 | 10620430 |
| immunostimulatory effect of azadirachtin in oreochromis mossambicus (peters). | the effect of azadirachtin, a triterpenoid derived from azadirachta indica on the immune response was studied in the freshwater teleost, o. mossambicus. bovine serum albumin (bsa) and sheep erythrocytes (srbc) were used as antigens to evoke immune response. the immune responses in fish were measured by quantifying antibodies produced and counting the peripheral blood leucocytes in control and experimental fish. in general, azadirachtin significantly enhanced the antibody response and leucocyte c ... | 2000 | 11395951 |
| oreochromis mossambicus (tilapia) corticotropin-releasing hormone: cdna sequence and bioactivity. | although hypothalamic corticotropin-releasing hormone (crh) is involved in the stress response in all vertebrate groups, only a limited number of studies on this neuroendocrine peptide deals with non-mammalian neuroendocrine systems. we determined the cdna sequence of the crh precursor of the teleost oreochromis mossambicus (tilapia) and studied the biological potency of the crh peptide in a homologous teleost bioassay. polymerase chain reaction (pcr) with degenerate and specific primers yielded ... | 2000 | 10718913 |
| leiperia cincinnalis sambon, 1922 (pentastomida) from nile crocodiles crocodylus niloticus in the kruger national park, south africa, with a description of the male. | a single male and several adult females of the pentastomid leiperia cincinnalis were recovered from the trachea of five of six nile crocodiles examined in 1995 and 1998. infective larvae, pre-adult males and females, as well as mature males, occurred in clusters in the pulmonary artery but infective larvae and pre-adult females were also occasionally taken from the lungs. irrespective of the developmental stage, the intensity of infection was 3, 6, 48, 72 and 79. sixty-four percent of eggs recov ... | 2000 | 10937664 |
| evidence for an osmoregulatory role of thyroid hormones in the freshwater mozambique tilapia oreochromis mossambicus. | the existing equivocal reports on the osmoregulatory role of triiodothyronine (t(3)) and thyroxine (t(4)) in teleosts prompted a reinvestigation of their osmoregulatory function in the euryhaline teleost oreochromis mossambicus. evidence is presented for thyroidal involvement in hydromineral balance in freshwater tilapia. dose- and tissue-related responses to various t(3) and t(4) concentrations were observed in the branchial and renal tissues. the branchial na(+),k(+)-atpase activity, known to ... | 2000 | 11078627 |
| the hexapeptide kp-102 (d-ala-d-beta-nal-ala-trp-d-phe-lys-nh(2)) stimulates growth hormone release in a cichlid fish (ooreochromis mossambicus). | abstract studies in mammals have shown that synthetic met-enkephalin derivatives, called growth hormone-releasing peptides (ghrps), stimulate growth hormone (gh) release. the present study was conducted to determine whether the ghrp, kp-102, specifically stimulates gh release in a teleost. tilapia (oreochromis mossambicus) were given a single intraperitoneal injection of kp-102 (d-ala-d-beta;-nal-ala-trp-d-phe-lys-nh(2)) or bovine ghrh(1-29)-amide or vehicle and blood was sampled at 1, 6 and 12 ... | 2000 | 11115782 |
| partial characterization of serum immunoglobulins of oreochromis mossambicus. | serum immunoglobulins of o. mossambicus were purified using chromatography methods--cm affinity gel blue chromatography followed by two step purification involving a combination of ion-exchange and gel filtration chromatography. studies revealed that o. mossambicus produces only one class of high molecular weight macroglobulin as determined by molecular sieving by sepharose cl 6-b. immunoelectrophoresis of purified o. mossambicus serum against rabbit anti o. mossambicus serum gave only a single ... | 2000 | 11116524 |
| differential effect of chromium compounds on the immune response of the african mouth breeder oreochromis mossambicus (peters). | the objective of this study was to investigate the effect of trivalent (chromic chloride) and hexavalent (potassium dichromate) forms of chromium in the african mouth breeder oreochromis mossambicus (peters), with reference to the humoral immune response and lymphoid cells/organs. the 96 h ld50 for hexavalent and trivalent chromium was found to be 75 and 1,000microg fish(-1), respectively. groups of fishes were injected intraperitoneally with 10, 1, 0.1 and 0.01% ld50 hexavalent and trivalent fo ... | 2000 | 11185752 |
| isolation and characterization of myostatin complementary deoxyribonucleic acid clones from two commercially important fish: oreochromis mossambicus and morone chrysops. | in mammals, skeletal muscle mass is negatively regulated by a muscle-derived growth/differentiating factor named myostatin (mstn) that belongs to the transforming growth factor-beta superfamily. although putative mstn homologs have been identified from several vertebrates, nonmammalian orthologs remained poorly defined. thus, we isolated and characterized mstn complementary dna clones from the skeletal muscle of the tilapia oreochromis mossambicus and the white bass morone chrysops. the nucleic ... | 2001 | 11250920 |
| effects of glucocorticoids on cartilage growth and response to igf-i in the tilapia (oreochromis mossambicus). | to study the effects of glucocorticoids and igf-i on the modulation of growth in the tilapia oreochromis mossambicus, we employed an epiceratobranchial cartilage radioisotope incorporation assay, wherein radiolabeled sulfate and thymidine uptakes are measured in vitro to indicate proteoglycan synthesis and cell proliferation, respectively. cartilage explants were cultured with cortisol or dexamethasone with or without recombinant bovine insulin-like growth factor-i. cortisol directly inhibited s ... | 2001 | 11254370 |
| effects of estrogens in vitro and in vivo on cartilage growth in the tilapia (oreochromis mossambicus). | to study the effects of estrogens on cartilage growth in the tilapia oreochromis mossambicus, an epiceratobranchial cartilage radioisotope incorporation assay was employed to measure proteoglycan synthesis and prechondrocyte proliferation by incorporation of radiolabeled sulfate and thymidine, respectively. cartilage explants were cultured with estrogens with or without recombinant bovine insulin-like growth factor-i (igf-i). in vitro experiments using the natural teleost estrogen, 17beta-estrad ... | 2001 | 11254371 |
| hepatocyte nuclear factors-1alpha, -1beta, and -3beta expressed in the gonad of tilapia (oreochromis mossambicus). | hepatocyte nuclear factors (hnfs) are upstream regulators of many liver-specific genes and are involved in many cellular functions in the body, but their existence, expression, and function in gonads are still poorly understood. here we report on the first cloning of partial cdnas of hnf-1alpha and -1beta and full hnf-3beta cdna from a tilapia (oreochromis mossambicus) liver cdna library. the deduced amino acid sequence of tilapia hnf-3beta has a 90 to 96% identity with those of other fishes (dw ... | 2001 | 11688983 |
| [ecophysiological adaptability of tropical water organisms to salinity changes]. | physiological response of tropical organisms to salinity changes was studied for some marine, estuarine and freshwater fishes (astyanax bimaculatus, petenia karussii, cyprinodon dearborni, and oreochromis mossambicus), marine and freshwater crustaceans (penaeus brasiliensis, penaeus schmitti and macrobrachium carcinus), and marine bivalves (perna perna, crassostrea rhizophorae, and arca zebra) collected from northeast venezuela. they were acclimated for four weeks at various salinities, and (1) ... | 2001 | 11795174 |
| tilapia glycoprotein hormone alpha subunit: cdna cloning and hypothalamic regulation. | the cdna encoding the glycoprotein alpha (gpalpha) subunit of tilapia (oreochromis mossambicus) was partially cloned using race-polymerase chain reaction (pcr) technique. the amplified cdna was found to be 583 bases long, and to consist of a portion of the signal peptide, the full sequence encoding the mature peptide (94 amino acids) and the 3' untranslated region. northern blot analysis revealed a single band of approximately 600 bp. alignment of the deduced amino acids of the mature protein sh ... | 2001 | 11500238 |
| heat shock stimulation of a tilapia heat shock protein 70 promoter is mediated by a distal element. | we reported previously that a tilapia (oreochromis mossambicus) heat shock protein 70 (hsp70) promoter is able to confer heat shock response on a reporter gene after transient expression both in cell culture and in microinjected zebrafish embryos. here we present the first functional analysis of a fish hsp70 promoter, the tihsp70 promoter. using transient expression experiments in carp epc (epithelioma papulosum cyprini) cells and in microinjected zebrafish embryos, we show that a distal heat sh ... | 2001 | 11368761 |
| isolation and characterization of a homologue of mammalian prolactin-releasing peptide from the tilapia brain and its effect on prolactin release from the tilapia pituitary. | in the tilapia (oreochromis mossambicus), as in many teleosts, prolactin (prl) plays a major role in osmoregulation in freshwater. recently, prl-releasing peptides (prrps) have been characterized in mammals. independently, a novel c-terminal rf (arginine-phenylalanine) amide peptide (carrasius rf amide; c-rfa), which is structurally related to mammalian prrps, has been isolated from the brain of the japanese crucian carp. the putative prrp was purified from an acid extract of tilapia brain by af ... | 2002 | 11884078 |
| rat ghrelin stimulates growth hormone and prolactin release in the tilapia, oreochromis mossambicus. | recently, ghrelin (ghr), a new peptide which specifically stimulates growth hormone (gh) release from the pituitary, was identified in the rat and human stomach. ghrelin has been shown to stimulate gh release by acting through a growth hormone secretagogue (ghs) receptor in the rat. the present study describes the in vitro effect of rat ghr on the release of gh and two forms of prolactin (prl(177) and prl(188)) in the tilapia, oreochromis mossambicus. rat ghr stimulated the release of gh in a do ... | 2002 | 12149581 |
| effects of insulin-like growth factors (igf-i and -ii) on growth hormone and prolactin release and gene expression in euryhaline tilapia, oreochromis mossambicus. | we investigated in vitro effects of insulin-like growth factors (igf-i and -ii) on growth hormone (gh) and prolactin (prl) release and gene expression in euryhaline tilapia, oreochromis mossambicus. pituitaries were removed from freshwater-acclimated adult males and incubated for 2-24h in the presence of human igf-i or -ii at doses ranging from 1-1000 ng/ml (0.13-130 nm). igf-i at concentrations higher than 10 ng/ml and igf-ii higher than 100 ng/ml significantly inhibited gh release after 8, 16, ... | 2002 | 12225763 |
| kidney-specific chloride channel, omclc-k, predominantly expressed in the diluting segment of freshwater-adapted tilapia kidney. | the kidney plays an important role in osmoregulation in freshwater teleosts, which are exposed to the danger of osmotic loss of na(+) and cl(-). however, ion-transport mechanisms in the kidney are poorly understood, and ion transporters of the fish nephron have not been identified thus far. from mozambique tilapia, oreochromis mossambicus, we have cloned a chloride channel, which is a homologue of the mammalian kidney-specific chloride channel, clc-k. the cdna of the channel, named omclc-k, enco ... | 2002 | 12427972 |
| influence of hypergravity on fish inner ear otoliths: ii. incorporation of calcium and kinetotic behaviour. | larval siblings of cichlid fish (oreochromis mossambicus) were subjected to hypergravity (hg; 3 g, 14 days) during development. following the transfer to 1 g (i.e., stopping the centrifuge) they were separated into normally and kinetotically swimming individuals (the latter performed spinning movements). during hg, the animals were maintained in aquarium water containing alizarin-complexone (ac), a fluorescent calcium tracer. densitometric measurements of ac uptake into inner ear otoliths (optic ... | 2002 | 12528672 |
| ambient salinity modulates the expression of sodium pumps in branchial mitochondria-rich cells of mozambique tilapia, oreochromis mossambicus. | na,k-atpase (sodium pumps) provide the primitive driving force for ion transport in branchial epithelial cells. immunoblots of epithelial homogenates of both seawater (sw)- and freshwater (fw)-adapted tilapia gills as well as rat brain homogenate, a positive control, revealed one major band with a molecular weight of about 100 kda. sw-adapted tilapia gills possessed larger (about 2-fold) amounts of sodium pumps compared with fw-adapted tilapia gills. (3)h-ouabain binding representing functional ... | 2003 | 12560598 |
| characterization of a piscirickettsiosis-like disease in hawaiian tilapia. | in 1994, tilapia (oreochromis mossambicus and sarotherodon melanotheron) in wild and farmed populations on oahu, hawaii, usa, began to die of an unknown disease that was similar but not identical to piscirickettsiosis in salmonids. only tilapia were affected. diseased tilapia often swam erratically and had trouble staying at depth. scattered cutaneous haemorrhage and exophthalmia were often noted. in many cases, fish were found dead with no clinical signs. gills exhibited epithelial hyperplasia ... | 2003 | 12691196 |
| nitrate removal in aquariums by immobilized pseudomonas. | biological denitrification of nitrate to nitrogen gas was examined in a freshwater and a marine aquarium. nitrate removal in the aquarium water was accomplished with denitrifiers immobilized in a freeze-dried, alginate-starch matrix. starch served as a bacterial carbon source and cellular matrix-strengthening filler. freeze-dried beads were placed in canisters through which nitrate-rich aquarium water was recirculated. the freshwater aquarium (100 l) contained goldfish (carassius auratus) at a t ... | 2003 | 12790671 |