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the use of electrophoresis of the water-soluble (sarcoplasmic) proteins of fish muscle to differentiate the closely related species of hake (merluccius sp.).1. electrophoretic separation patterns of water-soluble proteins of five species of hake, merluccius merluccius; m. australis; m. productus; m. bilinearis and m. angustimanus, have been examined. 2. the patterns were reproducible within each species and distinguishable from one another. 3. the frequency distribution of numbers of vertebrae and of gillrakers were obtained for the same fish. 4. only the electrophoretic patterns gave an unequivocal identification of the species.1978318280
[effect of various fish meals on disaccharidases and alkaline phosphatase of the small intestine in rats].female rats of the wistar strain, weighing 40-60 g were used to study the effect of fish meals (coryphaenoides rupestris, chimaera monstruosa and merluccius merluccius) on the disaccharidases and alkaline phosphatase in the small intestine in relation to the control group which consumed casein. fish meal diets diminished lactase and alkaline phosphatase activity, the latter being most remarkable in animals fed ch. monstruosa meal, while no statistical variations in maltase and sucrase activity w ...19826818626
influence of certain fish meals on (na+ + k+)-atpase and ca2+-atpase activity in rat small intestine.the effect of high-protein content fish meal on (na+ + k+)-atpase and ca2+-atpase activity in rat small intestine was studied. 5 groups of wistar rats, weighing between 40-60 g, were fed diets with 12% protein content of dry matter for 10 days. the protein source was casein for the control group and fish meal derived from coryphaenoides rupestris, chimaera monstruosa and merluccius merluccius for the test group. the results show a decrease in (na+ + k+)-atpase and a rise in ca2+-atpase activity ...19836312513
[anisakids and human anisakiasis. 2. investigation of the anisakids of commercial fish in the district of paris].1,173 fish belonging to 13 species were examined during 1986-1987, during a survey conducted among commercial marine fish in the paris area (france). anisakis simplex l3 were found in 10 different species, and most frequently in herring (clupea harengus) 82.55% of 682 fish, red fish (sebastes marinus) 86.11% of 36 fish, and hake (merluccius merluccius) 88.57% of 35 fish, and in high number (average 8.8, 18.4 and 31 l3 per fish). pseudoterranova decipiens l3 were recovered from 7 different specie ...19883190121
preliminary study of the effects of some physical parameters on the stability of methylmercury in biological samples.the effects of storage conditions (long-term storage of wet samples in a deep-freeze or thermal cycling), freeze-drying and gamma-irradiation at 1 and 5 mrad on the stability of methylmercury in some biological samples were investigated. methylmercury was determined by volatilization separation followed by gas chromatography and by ion-exchange separation of inorganic and organic species followed by measurement by cold vapour atomic absorption spectrometry (cvaas). total mercury was determined b ...19921580416
morphological analysis of the fish heart ventricle: myocardial and connective tissue architecture in teleost species.light and scanning electron microscopy were used to study the structure of the heart ventricle in three species of marine teleost fishes: the hake (merluccius merluccius), the angler fish (lophius piscatorius) and the sea bream (pagellus centrodontus). our findings show the ventricle to be shaped differently in each species: tubular in the hake, saccular in the angler fish and pyramidal in the sea bream. from a structural viewpoint, interest was centered on two aspects: organization of the myoca ...19957541184
[fat, protein and caloric content of different fish, seafood and mollusks, atlantic and mediterranean habitually consumed in the south of spain].to evaluate in a systematic manner, the fat, protein, caloric, and fatty acids contents of 35 species of fish, seafood and mollusks, usually consumed in andalucía (spain).19968962904
the distribution and abundance of lernaeocera lusci (copepoda) on hake (merluccius merluccius) and bib (trisopterus luscus) (teleostei).distribution patterns, mean intensity and prevalence of lernaeocera lusci (copepoda, pennellidae) in its hosts trisopterus luscus (teleostei, gadidae) and merluccius merluccius (teleostei merlucciidae) were examined, together with parasitic abundance and aggregation in relation to the size of the host. the mean parasite abundance and variance to mean abundance ratio increased with host size, suggesting that the accumulation of this parasite had no noticeable impact on the structure of either hos ...19969024889
genomic identification of anisakis simplex isolates.rapd technique was used to differentiate individuals of anisakis simplex obtained from merluccius merluccius, phycis blennoides, conger conger and lepidorhombus boscii, from the north atlantic ocean. the amplification patterns of the host dna controls were markedly different from those obtained for the parasitic material. no variation within the same host was detected. the amplification patterns for larvae obtained from fish of the same genus were somewhat different. the amplification patterns o ...19979166445
lipid, protein, and calorie content of different atlantic and mediterranean fish, shellfish, and molluscs commonly eaten in the south of spain.we undertook a systematic evaluation of the lipid, protein, calorie, and fatty acid composition in 35 species of fish, shellfish and molluscs commonly consumed throughout the four seasons of the year in andalusia, spain. using a portion of muscle tissue the following were measured in each study unit: total lipids (extraction using folch's method and gravimetry), protein concentration (kjehldal's method), total calories (direct calorimetry), and composition of fatty acids (gas chromatography). th ...19979258553
thermal gelation of chicken, pork and hake (merluccius merluccius, l) actomyosin.response surface methodology compared the effects of protein concentration (pc, 5-15 mg ml(-1)), ionic strength (nacl concentration, is, 0.1-0.7 m) and ph (5.2-7.5) on the gelation properties (penetration test) of natural actomyosin (nam) from chicken, pork and hake muscle. results indicated that, for ln work of penetration (ln wp), models had r(2) of 0.705 (p < 0.01) for nam from pork, 0.813 (< 0.001) for nam from chicken and 0.264 from hake (p > 0.05). the maximum work of penetration did not d ...199722062626
heavy metal accumulation in mullus barbatus, merluccius merluccius and boops boops fish from the aegean sea, turkey.the accumulation behaviour of copper, zinc, cadmium and lead concentrations in flesh, gills, liver and gonads of three commercial fish mullus barbatus, merluccius merliccius and boops boops from the aegean sea in turkey have been studied. copper, zinc and lead concentrations in flesh were found low, while in gonads cd was found high. liver showed higher concentration of cu than other fish organs. gonads and liver recorded high concentrations of cd. liver and gills revealed high levels of pb as c ...199910719583
ultrastructure of the myofibrillar component in cod (gadus morhua l.) and hake (merluccius merluccius l.) stored at -20 degrees c as a function of time.transmission electron microscopy and image analysis techniques were used to study the ultrastructure of the myofibrillar component in cod and hake muscle stored at -20 degrees c for varying periods of time. cod muscle showed a deformation of the hexagonal array of thick filaments with the storage time, reflected in an increase in the eccentricity value, a parameter defined to measure changes in the ratio of maximum to minimum hexagon diameter, and an increase in the cross-linkings between the fi ...199910552726
isolation of mycobacteria from frozen fish destined for human consumption.mycobacteria were isolated from defrost water and tissue of sole (solea solea), hake (merluccius merluccius), cod (gadus morhua), ling (genypterus blacodes), and monkfish (lophius piscatorius) on löwenstein-jensen medium after incubation at different temperatures. samples of frozen fish were obtained under sterile conditions inside a refrigeration chamber (-18 to -22 degrees c) in a wholesale market from which these products are distributed to shops for retail sale and human consumption.200010919833
organic and inorganic arsenic and lead in fish from the south adriatic sea, italy.total, organic and inorganic arsenic and lead were measured from the muscle of different kinds of fish: skate (raje spp.), hake (merluccius merluccius) and blue whiting (micromesistius poutassou), caught in the south adriatic sea (south italy) in the period june-august 1995. the highest levels of total arsenic were found in skate (raje spp.) whose values ranged from 14.4 to 61.5 mg/kg ww, followed by blue whiting (m. poutassou) and hake (m. merluccius) that showed lower levels ranging from 8.5 t ...200011091789
characterization and partial sequencing of species-specific sarcoplasmic polypeptides from commercial hake species by mass spectrometry following two-dimensional electrophoresis.the merluccidae family comprises marine species, some of them of high commercial value and others less appreciated, whose commercialization in europe under the generic name of "hake" is highly remarkable. the potential of proteomics was employed in this study with the aim of achieving the differential characterization of five different hake species: merluccius merluccius (european hake), m. australis (southern hake), m. hubbsi (argentinian hake), m. gayi (chilean hake), and m. capensis (cape hak ...200111386668
genetic markers in the study of anisakis typica (diesing, 1860): larval identification and genetic relationships with other species of anisakis dujardin, 1845 (nematoda: anisakidae).genetic variation at 21 gene-enzyme systems was studied in a sample of an adult population of anisakis typica (diesing, 1860) recovered in the dolphin sotalia fluviatilis from the atlantic coast of brazil. the characteristic alleles, detected in this population, made it possible to identify as a. typica, anisakis larvae with a type i morphology (sensu berland, 1961) from various fishes: thunnus thynnus and auxis thazard from brazil waters, trachurus picturatus and scomber japonicus from madeiran ...200211912342
mercury, arsenic, lead and cadmium in fish and shellfish from the adriatic sea.the aim was to measure concentrations of total mercury, total arsenic, lead and cadmium in common edible fresh fish and shellfish from various areas of the adriatic sea. estimates of intake of these elements were made through seafood consumption by the general population. samples were either wet digested for mercury and arsenic, or dry ashed for lead and cadmium analysis. mercury was measured by cold vapour atomic absorption spectrometry (cv aas) and arsenic, lead and cadmium by electrothermal a ...200312623648
study of the distribution of trace elements in six species of marine organisms of the ligurian sea (north-western mediterranean)--comparison with previous findings.this paper presents the results of the determination of several trace elements (total and organic mercury, selenium, cadmium, lead, copper, manganese and zinc) in six species of marine organisms (nephrops norvegicus, engraulis encrasicolus, mullus barbatus, mullus surmuletus, merluccius merluccius, and sarda sarda), sampled in the ligurian sea (north-west mediterranean) during a three-year period, from 1998 to 2000. the species analysed (388 samples) are the same studied in previous investigatio ...200415347201
genetic identification of nine hake species for detection of commercial fraud.this study describes the 5s rrna gene as a simple and reliable one-step pcr-based genetic marker that allows unambiguous identification of merluccius paradoxus, merluccius senegalensis, merluccius australis, merluccius gayi, merluccius bilinearis, merluccius hubbsi, and macruronus magellanicus. the marker is based on differences in length at the nontranscribed spacer within the 5s rrna genes and is robust enough for identification of processed products, such as fish fingers and other precooked c ...200415633688
alkylphenols and alkylphenol ethoxylates contamination of crustaceans and fishes from the adriatic sea (italy).this paper presents the results of an investigation on the occurrence of alkylphenols (aps) and their ethoxylates (apes) in 8 edible marine species from the adriatic sea and tries to estimate the corresponding intake for the italian population. two crustaceans, nephrops norvegicus (norway lobster) and squilla mantis (spottail mantis shrimp), plus six fish species, engraulis enchrascicolus (anchovy), scomber scombrus (atlantic mackerel), merluccius merluccius (european hake), mullus barbatus (red ...200515833488
evidence for a new species of anisakis dujardin, 1845: morphological description and genetic relationships between congeners (nematoda: anisakidae).in the present study, a new biological species of anisakis dujardin, 1845, was detected in kogia breviceps and k. sima from west atlantic waters (coast of florida) on the basis of 19 (nuclear) structural genes studied by multilocus allozyme electrophoresis. fixed allele differences at 11 enzyme loci were found between specimens of both adults and larvae of the new species and the other anisakis spp. tested. reproductive isolation from a. brevispiculata dollfus, 1968 was demonstrated by the lack ...200516025205
biogenic amine index for freshness evaluation in iced mediterranean hake (merluccius merluccius).biogenic amine accumulation was studied during the ice storage of mediterranean hake. sensory analysis and counts of shewanella, pseudomonas, enterobacteria, psychrotrophic, and mesophilic bacteria provided complementary information on hake spoilage. putrescine and cadaverine were the main amines accumulated, whereas histamine and tyramine were minor amines but had qualitative interest from the hygienic point of view. although all biogenic amines were less abundant than in pelagic fish, they may ...200516300084
monitoring the prestige oil spill impacts on some key species of the northern iberian shelf.selected key components of the continental shelf benthic and demersal communities were monitored for the two years following the prestige oil spill (pos) in order to identify the possible ecological effects of the oil. this work includes the first results regarding changes in abundance, distribution and food habits of hake (merluccius merluccius), four-spot megrim (lepidorhombus boscii), norway lobster (nephrops norvegicus) and pandalid shrimp (plesionika heterocarpus) populations of galician an ...200616343555
cell and tissue biomarkers in mussel, and histopathology in hake and anchovy from bay of biscay after the prestige oil spill (monitoring campaign 2003).in order to assess the biological effects of the prestige oil spill (pos), mussels (mytilus galloprovincialis), european hake (merluccius merluccius) and european anchovy (engraulis encrasicolus) were sampled between april and september 2003 in various geographical areas of bay of biscay: galicia, central cantabrian and east cantabrian. in mussels, several cell and tissue biomarkers were measured: peroxisome proliferation as induction of acyl-coa oxidase (aox) activity, lysosomal responses as ch ...200616271373
polycyclic aromatic hydrocarbons in marine organisms from the adriatic sea, italy.polycyclic aromatic hydrocarbons (pahs) were analyzed in bivalves (mitylus galloprovincialis), cephalopods (todarodes sagittatus), crustaceans (nephrops norvegicus) and fish (mullus barbatus, scomber scombrus, micromesistius poutassou, merluccius merluccius) in several pools coming from the central adriatic sea. these marine organisms were selected because of their multitude, wide distribution and common use in the italian diet, they were sampled and analyzed during the year 2004. acenaphthene, ...200716962639
polycyclic aromatic hydrocarbons in marine organisms from the gulf of naples, tyrrhenian sea.polycyclic aromatic hydrocarbons (pahs) were determined by an hplc method with fluorescence detection in bivalves (mitylus galloprovincialis), cephalopods (todarodes sagittatus), crustaceans (aristeus antennatus), and fish (mullus surmeletus, scomber scombrus, micromesistius poutassou, and merluccius merluccius) caught in the gulf of naples (tyrrhenian sea, italy). anthracene, fluoranthene, pyrene, benz(a)anthracene, chrysene, benzo(b)fluoranthene, and benzo(k)fluoranthene were detected, at diff ...200717288448
integrating genetic and parasitological approaches in the frame of multidisciplinary fish stock analysis.to assess fish stocks boundaries and state, the tools of population genetics have been widely used, contributing to the evaluation of relevant parameters such as the identification of stock boundaries, the assessment of gene flow and the estimation of effective population size. also, increasing evidences show that the monitoring of the genetic diversity level is a reliable method to check the status of fish stocks. however, genetics cannot answer all the questions. for example, in high gene flow ...200718410069
cholesterol oxidation is increased and pufa decreased by frozen storage and grilling of atlantic hake fillets (merluccius hubbsi).fresh fillets of atlantic hake were stored at -18 degrees c for 120 days and changes in lipid composition and the formation of cholesterol oxidation products (cop) during storage and subsequent grilling were evaluated. fresh hake showed low cop levels (8.0 microg/g, dry basis); however, a significant increase in cop (p < 0.02) and a concomitant decrease in the cholesterol and polyunsaturated fatty acids content during frozen storage and after grilling were observed. the main cholesterol oxides p ...200717492472
an optimized digestion method coupled to electrochemical sensor for the determination of cd, cu, pb and hg in fish by square wave anodic stripping voltammetry.an optimized digestion method coupled to electrochemical detection to monitor lead, copper, cadmium and mercury in fish tissues was developed. square wave anodic stripping voltammetry (swasv) coupled to disposable screen-printed electrodes (spes) was employed as fast and sensitive electroanalytical method for heavy metals detection. different approaches in digestion protocols were assessed. the study was focused on atlantic hake fillets because of their wide diffusion in the human nutrition. bes ...200919064103
effect of gutting on microbial loads, sensory properties, and volatile and biogenic amine contents of european hake (merluccius merluccius var. mediterraneus) stored in ice.the effect of gutting on sensory, microbiological, and chemical properties of european hake (merluccius merluccius var. mediterraneus) stored in ice was studied. gutting of hake noticeably affected the development of gram-negative bacteria: counts of enterobacteriaceae, shewanella putrefaciens, and pseudomonas throughout ice storage were higher in gutted than in ungutted samples. these differences in microbial loads were also reflected in the lower sensory scores of both raw and cooked hake, in ...200919722399
metals (hg, pb, cu, and zn) bioaccumulation in sediment, fish, and human scalp hair: a case study from the city of mersin along the southern coast of turkey.this study investigates mercury, lead, copper, and zinc concentrations in six most frequently consumed fish species (120 samples), sediments (20 samples) taken from karaduvar fishing area where fish species live, and mersin port as a contrary region, and human scalp hair for people regularly consuming these fish species (50 samples) and non-fish-eaters (15 samples) in mersin, turkey. on taking living environment into account, the fish groups include pelagic species of liza saliens, liza aurata, ...201019777169
the essential and toxic elements in tissues of six commercial demersal fish from eastern mediterranean sea.the essential elements and contaminants (pb and cd) were determined seasonally in the muscle and liver of six demersal fish species sparus aurata, chelidonichthys lucernus, upeneus molluccensis, solea solea, merluccius merluccius and saurida undosquamis from the iskenderun bay, eastern mediterranean sea. the concentrations in muscles of fish species were 474-1534 mg/kg for na, 574-3374 mg/kg for k, 72.7-496 m/kg ca, 94.1-210 mg/kg for mg. muscle accumulated the lowest levels of elements. trace e ...201020214948
genetic variability of anisakis simplex s.s. parasitizing european hake (merluccius merluccius) in the little sole bank area in the northeast atlantic.in this study, we researched the presence of anisakids in specimens of merluccius merluccius caught in the area of little sole bank, in the northeast atlantic, and found that 100% of the european hake examined were infected and showed high average values of abundance (976.88) and intensity (976.88). the larvae were identified in morphological terms as morphotype type i and in molecular terms as anisakis simplex s.s via polymerase chain reaction (pcr) restriction fragment length polymorphism of t ...201020694563
the biology and fisheries of european hake, merluccius merluccius, in the north-east atlantic.the aim of this chapter is to review the biology and fishery, including the management, of european hake in the north-east atlantic. the european hake is widely distributed throughout the north-east atlantic, from norway in the north to the guinea gulf in the south, and throughout the mediterranean and black sea, being more abundant from the british isles to the south of spain. in this area, ices (international council for the exploration of the sea) recognises the existence of two stocks: the n ...201020959157
application of enzymatic probe sonication extraction for the determination of selected veterinary antibiotics and their main metabolites in fish and mussel samples.a new method based on enzymatic probe sonication extraction prior to high-performance liquid chromatography (hplc) has been developed for the determination of 11 antibiotics (drugs) and the main metabolites of five of them in fish tissue and mussel samples. the analytes belong to four different classes of antibiotics (sulfonamides, tetracyclines, penicillins and amphenicols). the analysed compounds were sulfadiazine (sdi) and n(4)-acetylsulfadiazine (ndi) metabolite, sulfamethazine (smz) and n(4 ...201020800727
enzymatic-microwave assisted extraction and high-performance liquid chromatography-mass spectrometry for the determination of selected veterinary antibiotics in fish and mussel samples.a new method based on enzymatic-microwave assisted extraction prior to high performance liquid chromatography (hplc) has been developed for the determination of 11 antibiotics (drugs) and the main metabolites of five of them in fish tissue and mussel samples. the analysed compounds were sulfadiazine (sdi), n(4)-acetylsulfadiazine (ndi), sulfamethazine (smz), n(4)-acetylsulfamethazine (nmz), sulfamerazine (smr), n(4)-acetylsulfamerazine (nmr), sulfamethoxazole (smx), trimetroprim (tmp), amoxicill ...201121190806
prolonged feed deprivation does not permanently compromise digestive function in migrating european glass eels anguilla anguilla.the effects of prolonged feed deprivation (40 days at 18° c) and re-feeding (30 days) on body mass, growth and the activity of selected pancreatic and intestinal enzymes were evaluated in migrating european glass eels anguilla anguilla by comparison with a control group fed to satiation with hake merluccius merluccius roe for the duration of the experiment. feed deprivation resulted in mass loss and a reduction in digestive function, as revealed by a decrease in the total and specific activities ...201121284636
toxic metal (pb, cd, as and hg) and organochlorine residue levels in hake (merluccius merluccius) from the marmara sea, turkey.toxic metals (pb, cd, as and hg) and organochlorine residue levels were measured in hake (merluccius merluccius) from the marmara sea. biota samples were collected by a trawling cruise of the r/v arar in august and december 2009. the concentrations of toxic metals varied between pb, 3.23-14.4; cd, <0.01-2.14; hg, 0.01-0.18 and as, 0.01-0.21 [formula: see text]g g(?-?1) dry wt. pb levels in the marmara sea were found to be higher than the critical limits set by the both turkish ministry of enviro ...201121336486
molecular characterization of hysterothylacium aduncum (nematoda: raphidascaridae) from different fish caught off the tunisian coast based on nuclear ribosomal dna sequences.larval forms of the genus hysterothylacium have been previously reported in teleost fish from the north african coasts of central mediterranean sea by morphological analysis. in the present study, samples identified morphologically as hysterothylacium aduncum (n = 62), from merluccius merluccius, trachurus mediterraneus and pagellus erythrinus from different geographical locations of the tunisian coasts, were genetically characterised by sequences of the first (its-1), the 5.8s and second (its-2 ...201121519865
two new species of clestobothrium (cestoda: bothriocephalidea), parasites of merluccius australis and m. hubbsi (gadiformes: merlucciidae) from the patagonian shelf of argentina, with comments on clestobothrium crassiceps (rudolphi, 1819).abstract: two new species of bothriocephalidean cestodes, clestobothrium splendidum sp. n. from merluccius australis (hutton) and clestobothrium cristinae sp. n. from merluccius hubbsi marini from the patagonian shelf of argentina, are described. clestobothrium splendidum can be typified by the following characteristics: a medium-sized strobila composed of410-528 proglottides that are much wider than long; 49-90 testes per mature proglottis, partially surrounding the ovary posteriorly; a transve ...201121776892
a case study of trace metals in suspended particulate matter and biota before wastewater treatment plant from the izmir bay, turkey.the concentrations of trace metals (cu, zn, mn, ni, and fe) from suspended particulate matter (spm) and biota in izmir bay (eastern aegean sea) were studied in order to evaluate the environmental impact of the anthropogenic metals before building of wastewater treatment plant. spm samples were collected in wet and dry periods from izmir bay. metal concentrations in spm (cu, 0.36-2.19; mn, 0.07-11.3; ni, 0.43-7.81; zn, 7.33-269; fe, 1.00-266-á++gdm(ôçë-ôçë3)) were comparable to those reported for ...201121701893
characterization of the pelagic fish community of the north-western and northern spanish shelf waters.this study demonstrates the utility of trawl data, collected during acoustic surveys of pelagic fish stocks as a way of confirming fish identification, to characterize the pelagic community, as well as allowing description and prediction of fish distribution patterns, based on data from spanish atlantic ocean shelf surveys during spring 2005-2011. the composition of the pelagic community is described, as well as spatial and temporal patterns of variation in both the community composition and the ...201324090545
global assessment of molecularly identified anisakis dujardin, 1845 (nematoda: anisakidae) in their teleost intermediate hosts.here, we present the its ribosomal dna (rdna) sequence data on 330 larvae of nematodes of the genus anisakis dujardin, 1845 collected from 26 different bony fish species from 21 sampling locations and different climatic zones. new host records are provided for anisakis simplex (rudolphi, 1809) sensu stricto (s.s.) and a. pegreffli campana-rouget et biocca, 1955 from anoplopoma fimbria (pallas) (santa barbara, east pacific), a. typica (diesing, 1860) from caesio cuning (bloch), lepturacanthus sav ...201323724731
proteomic profiling and characterization of differential allergens in the nematodes anisakis simplex sensu stricto and a. pegreffii.the parasite species complex anisakis simplex sensu lato (anisakis simplex sensu stricto; (a. simplex s.s.), a. pegreffii, a. simplex c) is the main cause of severe anisakiasis (allergy) worldwide and is now an important health matter. in this study, the relationship of this anisakis species complex and their allergenic capacities is assessed by studying the differences between the two most frequent species (a. simplex s.s., a. pegreffii) and their hybrid haplotype by studying active l3 larvae p ...201424723494
an evolutionary preserved intergenic spacer in gadiform mitogenomes generates a long noncoding rna.vertebrate mitogenomes are economically organized and usually lack intergenic sequences other than the control region. intergenic spacers located between the trna(thr) and trna(pro) genes ("t-p spacers") have been observed in several taxa, including gadiform species, but information about their biological roles and putative functions is still lacking.201425145347
molecular cloning and measurement of telomerase reverse transcriptase (tert) transcription patterns in tissues of european hake (merluccius merluccius) and atlantic cod (gadus morhua) during aging.telomerase is a reverse transcriptase ribonucleoprotein that maintains the ends of linear chromosomes. this enzyme plays a major role in cell processes like proliferation, differentiation and tumorigenesis, being associated with aging and survival of species. in this study, the gene coding for tert (telomerase reverse transcriptase) of two commercial fish species, european hake (merluccius merluccius) and atlantic cod (gadus morhua), has been partially cloned. a fragment of 1581bp (hake) and 633 ...201424607378
molecular and epidemiological data on anisakis spp. (nematoda: anisakidae) in commercial fish caught off northern sardinia (western mediterranean sea).anisakiasis is a fish-borne zoonosis caused by third stage larvae of the nematode anisakis sp. present in fish or cephalopods. this is the first contribution to the molecular identification and epidemiology of anisakis spp. in commercial fish from the gulf of asinara (sardinia, western mediterranean sea). between april 2006 to november 2011, 777 specimens of 10 fish species (engraulis encrasicolus, merluccius merluccius, micromesistius poutassou, phycis blennoides, sardina pilchardus, sardinella ...201424630706
ecology and genetic structure of zoonotic anisakis spp. from adriatic commercial fish species.consumption of raw or thermally inadequately treated fishery products represents a public health risk, with the possibility of propagation of live anisakis larvae, the causative agent of the zoonotic disease anisakidosis, or anisakiasis. we investigated the population dynamics of anisakis spp. in commercially important fish-anchovies (anisakis), sardines (sardina pilchardus), european hake (merluccius merluccius), whiting (merlangius merlangus), chub mackerel (scomber japonicus), and atlantic bl ...201424317085
prevalence of anisakid nematode larvae infecting some marine fishes from the libyan coast.this study examined eight hundred ninety six marine fishes belonging to nine different fish species; synodus saurus; merluccius merluccius; trachurus mediterraneus; serranus cabrilla; mullus surmuletus; diplodus annularis; spicara maena; siganus rirulatus and liza ramada. the fishes were bought from fish markets at five different sites on libyan coast, from january to december 2013, for study the anisakids larvae among them. the results showed that 344/896 fishes (38.4%) were infected with anisa ...201526939239
removal of anisakis simplex allergens from infected fish during the washing step of surimi production.the washing operation of fish muscle is one of the key steps in the production of surimi. the aim of this study was to assess in parasitised minced fish the effect of the washing steps on the allergen removal of anisakis simplex and on protein yield during surimi processing. experimentally infected hake (merluccius merluccius) (50 anisakis simplex s.s l3 larvae per 100 g of muscle) underwent three successive washing steps with water, phosphate buffer (20 mmol l(-1) ), sodium bicarbonate (60 mmol ...201525378259
genetic identification and distribution of the parasitic larvae of anisakis pegreffii and anisakis simplex (s. s.) in european hake merluccius merluccius from the tyrrhenian sea and spanish atlantic coast: implications for food safety.the consumption of the hake merluccius merluccius is widespread in european countries, where this fish has a high commercial value. to date, different larval species of anisakis have been identified as parasites in m. merluccius from european waters, anisakis pegreffii and anisakis simplex (s. s.) being the two most common. the aim of the study is to present data on the occurrence of anisakis spp. larvae in the viscera and flesh of m. merluccius. consequently, the distribution and infection rate ...201525584776
strategies to increase the hygienic and economic value of fresh fish: biopreservation using lactic acid bacteria of marine origin.in this work we describe the development of a biopreservation strategy for fresh fish based on the use of bacteriocinogenic lab of marine origin. for this purpose, two multibacteriocinogenic lab strains, lactobacillus curvatus bcs35 and enterococcus faecium bnm58, previously isolated from fish and fish products were selected owing to their capability to inhibit the growth of several fish-spoilage and food-borne pathogenic bacteria. two commercially important fish species were chosen, young hake ...201626890359
first record of anisakis simplex third-stage larvae (nematoda, anisakidae) in european hake merluccius merluccius lessepsianus in egyptian water.the prevalence of infection and the identification of anisakid larvae in european hake merluccius merluccius lessepsianus from hurghada city, red sea governorate, egypt, were investigated. fish samples were collected during the period of february and november 2014. twenty-two (36.66%) out of sixty examined fish specimens were found to be naturally infected with anisakis type i larvae mostly found as encapsulated larvae in visceral organs. there was a positive relationship between host length/wei ...201626925257
occurrence of nematodes of the genus anisakis in mediterranean and atlantic fish marketed in sardinia.anisakiasis is a gastrointestinal fish-borne zoonosis caused by the ingestion of third stage larvae of the genus anisakis. between january and december 2013, 1112 specimens of four commercial fish species (engraulis encrasicolus, merluccius merluccius, scomber colias and trachurus mediterraneus) marketed in sardinia (italy) were examined for anisakis sp. the overall prevalence of anisakis spp larvae was 39.9%, all morphologically identified as type i. scomber colias showed the highest prevalence ...201728299289
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