Publications

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effect of various sorts of ion on the sensoria of the labyrinth of eel (anguilla japonica). 195114884175
anatomical studies on the labyrinth of anguilla japonica. 195114884173
studies in neurosecretion. i. preoptico-subcommissural neurosecretory system in the eel (anguilla japonica). 195414380182
studies in neurosecretion. iii. nuclear secretion in the cells of the preoptic nucleus in the eel (anguilla japonica). 195513241431
electrical activity of the caudal neurosecretory cells in the eel anguilla japonica with special reference to synaptic transmission. 196213956803
hemoglobins from erythrocytes of eel, anguilla japonica. 196414135455
malformed caudal neurosecretory system in the eel, anguilla japonica. 19655862488
development of the caudal and hypothalamic neurosecretory systems of the eel, anguilla japonica. 19655862487
renin and euryhalinity in the japanese eel, anguilla japonica. 19664306796
an electron microscopic study of the thyroid gland of the silver eel, anguilla japonica. (a part of phylogenic studies of the fine structure of the thyroid). 19666008494
[on the green chromoprotein of the blood of the japanese eel (anguilla japonica)]. 19674229302
effects of hypophysectomy and urophysectomy on water and sodium transport in isolated intestine and gills of japanese eel (anguilla japonica). 19676072677
on the fine structure of corpuscles of stannius of the eel, anguilla japonica. 19675591460
effects of acth and cortisol on water movement in isolated intestine of the eel, anguilla japonica. 19684301352
hemoglobins from erythrocytes of the eel, anguilla japonica. ii. further studies on the properties of two hemoglobins. 19685655084
effects of hypophysectomy and salinity change on plasma cortisol concentration in the japanese eel, anguilla japonica. 19694314473
development and degeneration of the chloride cell during seawater and freshwater adaptation of the japanese eel, anguilla japonica. 19705412831
plasma cortisol concentration and the rate of intestinal water absorption in the eel, anguilla japonica. 19714327140
monamine oxidase in the hypothalamo-hypophysial region of the teleosts, anguilla japonica and oryzias latipes. 19715541845
hormonal effect on na-k-atpase activity in the gill of japanese eel, anguilla japonica, with special reference to seawater adaptation. 19724265600
ultimobranchial calcitonin of the eel, anguilla japonica. 19724678354
fine structure of the lateral-line organ of the common eel, anguilla japonica. 19724334619
plasma renin activity in japanese eels (anguilla japonica) adapted to seawater or in dehydration. 19734724758
some factors regulating water intake by the eel, anguilla japonica.1. internal as well as external factors affecting water ingestion in the eel were analysed using oesophagus-cannulated eels. 2. acute withdrawal of the blood induced an immediate drinking response in the freshwater eel, whereas infusion of a large amount of hypertonic saline interrupted the copious drinking observed in the seawater eel. 3. the freshwater eel responded to slow infusion of hypertonic nacl solution by constant drinking. 4. inhibition of drinking was observed in the seawater eel by ...19744443754
the effects of organic phosphates on the oxygen equilibria of two distinct hemoglobins of the eel, anguilla japonica. 19744545251
the effects of bovine prolactin, sea water and environmental calcium on water influx in isolated gills of the euryhaline teleosts, anguilla japonica and salmo gairdnerii. 19744153722
[experimental studies on the second intermediate hosts of clonorchis sinensis: iii. observations on the relationship between clavate cells of epidermis and infectivity of metacercariae of clonorchis sinensis in fresh-water fish]in order to know relationship between the clavate cells of epidermis and infectivity of metacercariae of c. sinensis. histological investigation of epidermis was carried out on the 26 species of fresh-water fish collected from the main rivers in korea. results were summarized as it follows: 1. in general clavate cell had a single nucleus at its center. however it was observed in liobagrus mediadiposalis and pelteobagrus fuluidraco that clavate cell had two nuclei with and independent so-called l ...197412913471
the effects of prolactin, cortisol and calcium-free environment on water influx in isolated gills of japanese eel, anguilla japonica. 1975241555
the cardiac cycle and the effects of neurohumors on myocardial contractility in the asiatic eel anguilla japonica, timm. & schle. 1975201
intestinal water transport and chloride pump in relation to sea-water adaptation of the eel, anguilla japonica. 1975240556
morphochemical analysis of mucosubstances in some epithelial tissues of the eel (anguilla japonica).in attempts to gain insight into the nature of mucosubstances in the eel epithelial tissues, the epidermis and epithelia lining the gill and intestine of the fish have been reacted for a series of light microscopic stainings of mucosaccharide and protein histochemistry under normal and experimental conditions. the present morphochemical analyses indicate that (1) the mucosubstances of the epidermis are found within two well differentiated cell types, goblet and clavate cells which elaborate a ne ...1975124717
karyotype conservation and difference in dna amount in anguilloid fishes.the two pacific anguilloid sishes anguilla japonica and astroconger myriaster, belonging to the different families, appear to have identical chromosome numbers (2n = 38) and karyotypes, including one pair of conspicuous heteromorphic chromosomes in females. cytophotometric measurements, however, indicate a considerable difference in dna content between the two species.1976935856
the effects of acetylcholine, biogenic amines and other vasoactive agents on the cardiovascular functions of the eel, anguilla japonica.the blood pressure in the cardinal vein, cardiac chambers and ventral and dorsal aortae have been recorded from conscious eels in water. at low doses, acetylcholine reduced the heart rate but increased the transbranchial differential blood pressure. the effects were abolished by atropine but not by tubocurarine. at doses in excess of 0.1 mug/kg, a secondary rise in arterial blood pressure was observed which could be abolished by bretylium, phentolamine or phenoxybenzamine. catecholamines did not ...1976932655
effects of glucagon on amino acid metabolism in japanese eels, anguilla japonica. 1977200519
the effect of hypophysectomy and prolactin treatment on the osmotic water influx into the isolated gills of the japanese eel (anguilla japonica). 1977871995
increased calcitonin levels during ovarian development in the eel, anguilla japonica. 1978750287
morphological changes in the esophageal epithelium of the eel, anguilla japonica, during adaptation to seawater.the esophageal epithelium of the japanese eel, anguilla japonica, was studied by light and electron microscopy. in freshwater-adapted eels, longitudinal folds of the mucosal surface are simple in form and lined by a stratified epithelium composed of mucous cells, filament- and ribosome-rich cells. mucous cells are numerous. the filament-rich cells form the outermost and the basal layers of the stratified epithelium and are scattered in the middle zone among the mucous cells. they are firmly boun ...1978688374
effect of glucagon on the metabolism of the eel, anguilla japonica. 1978689355
effect of cortisol on the metabolism of the eel, anguilla japonica. 1978689354
the respiratory metabolism of the japanese eel, anguilla japonica: effects of ambient oxygen, temperature, season, body weight, and hypophysectomy. 1978669252
effect of hypophysectomy on the chemical composition and intermediary metabolism of the japanese eel, anguilla japonica. 1978208922
effect of eel calcitonin on serum electrolytes in the eel anguilla japonica. 1978631554
angiotensin and water intake in the japanese eel, anguilla japonica. 1979478280
histological and cytological studies on the liquor contacting peptidergic neurons in the preoptic nucleus of the japanese eel (anguilla japonica).liquor contacting peptidergic neurons (lcpns) in the preoptic nucleus of the japanese eel (anguilla japonica), are investigated submacroscopically, light microscopically, electron microscopically (transmission and scanning) and histochemically. lcpns appear in 8--13 per cent of all neurons constituting the preoptic nucleus and their cytoplasm contains many secretory granules stained by aldehyde-thionin or chrome hematoxylin. lcpns have an epithelial cell-like polarity and their cytoplasmic organ ...1979539883
karyological confirmation of conspicuous zw sex chromosomes in two species of pacific anguilloid fishes (anguilliformes: teleostomi).leukocyte and kidney cell cultures were used to examine the sex chromosomes of japanese freshwater eels (anguilla japonica) and conger eels (astroconger myriaster). the conspicuous presence of zw sex chromosomes, due to a drastic reduction in size of the w, was confirmed in both species, where the z was the largest metacentric and the w was the smallest metacentric in a diploid karyotype of 38 chromosomes in both species. these species are the first examples of the zw type of heteromorphism in e ...1979761483
relationship between essential fatty acid requirements of aquatic animals and the capacity for bioconversion of linolenic acid to highly unsaturated fatty acids.1. [1-14c]linolenic acid was injected into the rainbow trout, salmo gairdnerii, ayu, plecoglossus altivelis, eel, anguilla japonica, red sea bream, chrysophrys major, rockfish, sebastiscus marmoratus, globefish, fugu rubripes rubripes and prawn, penaeus japonicus (molting stage d"1-d2), and the bioconversion of linolenic acid (18:3 omega 3) to highly unsaturated fatty acids such as eicosapentaenoic (20:5 omega 3) and docosahexaenoic (22:6 omega 3) acids was investigated. 2. linolenic acid was co ...1979233802
[the wormicidal substances of fresh water fishes on clonorchis sinensis: ii. preliminary research on the wormicidal substances from mucous substances of various fresh water fishes]the present work which was investigated in july 1979, was to observe the wormicidal effects of the external mucous substances of 9 species of fresh water fishes (cyprinus carpio, parasilurus asotus, anguilla japonica, ophicephalus argus, carassius carassius; golden crusian carp, misgurnus anguillicaudatus, zacco platypus, pseudorasbora parva and carassius carassius; crusian carp) on cercaria, liberated metacercaria and adult of clonorchis sinensis. the mucous substances extracted by ether from t ...198012902746
the multiple hemoglobins of the japanese eel, anguilla japonica. molecular basis for hemoglobin multiplicity and the subunit interactions. 19807400154
reconstruction of the gill from single-cell suspensions of the eel, anguilla japonica.minute gill arches, about 2 mm in length and with 17-20 pairs of filaments, were reconstructed from single-cell suspensions of the adult eel gill. mixed populations of the dissociated cells were cultured with growth medium (ph 7.2-7.4) either in a rose chamber or in a roller tube at 20 degrees c. single cells began to aggregate within 1 h into small cell clusters, which increase in size with selective adhesion of free cells. melanin cells were frequently observed in the aggregates. cell aggregat ...19807369391
further studies on the absence of hypocalcemic effects of eel calcitonin in the eel, anguilla japonica. 19817215772
electron microscope study of vertebrate liver innervation.liver fine structure was studied in various groups of vertebrates to reveal intrahepatic nerves. nerve fibers were found in the connective tissue of the liver in all mammals, birds, and reptiles studied (japanese monkey, crab-eating monkey, rabbit, guinea pig, rat, golden hamster, pigeon, japanese quail, and turtle, pseudemys scripta). nerve fibers also made direct contact with hepatocytes in these animals except for the rat and the golden hamster. intrahepatic nerves were rare or absent in amph ...19817316686
vibrio vulnificus biogroup 2: new biogroup pathogenic for eels.clinical and nonclinical isolates of the lactose-positive vibrio vulnificus were compared with vibrio strains isolated from lesions on eels (anguilla japonica) cultured commercially in japan. strains were compared phenotypically and antigenically, for pathogenicity to mice and eels, and for genetic relatedness. the strains isolated from diseased eels differed phenotypically from the original species description of v. vulnificus in that they were negative for indole production, ornithine decarbox ...19827138004
hypocalcemic effect of homologous angiotensin-like substances produced by the renin-like enzyme in the corpuscles of stannius in the japanese eel anguilla japonica. 19827044887
drinking induced by angiotensin ii in fishes.among 20 species of freshwater fishes examined, pseudorasbora parva, rhodeus ocellatus, cobitis anguillicaudatus, carassius auratus, oryzias latipes, gambusia affinis, and gyrinocheilus anymonieri were found to drink water like seawater fishes, while 13 remaining species did not drink. for fish species found exclusively in fresh water, angiotensin ii (aii) treatment did not induce drinking. in contrast, those freshwater fishes which survive in estuarine brackish water (leuciscus hakonensis, c. c ...19836840524
immunocytochemical identification of thyrotropin (tsh)-producing cells in pituitary glands of several species of teleosts with antiserum to human tsh beta subunit.a rabbit antiserum raised against human thyrotropin (tsh) beta subunit (anti-human beta-tsh) was used to identify tsh cells in the pituitaries of five species of teleosts by means of the unlabeled antibody peroxidase-antiperoxidase (pap) technique. the anti-human beta-tsh reacted only with polygonal basophils located in the rostral pars distalis (rpd) in the pituitaries of sexually immature eel (anguilla japonica). in the pituitaries of medaka (oryzias latipes) and white-edged rockfish (sebastes ...19836342806
effects of prolactin and environmental calcium on osmotic water permeability of the gills in the eel, anguilla japonica.the effects of prolactin and environmental calcium on branchial osmotic water permeability were examined using gill arches isolated from freshwater-adapted eels (anguilla japonica). the gill arches were incubated for 1 hr in deionized water (dw) or in various concentrations of electrolyte solutions and the rate of osmotic water influx was calculated from the weight change. addition of 1.0 mm calcium to the incubation medium (dw) resulted in an almost instantaneous reduction in the rate of water ...19846698394
purification and some properties of two anionic trypsins from the eel (anguilla japonica).two anionic enzymes, designated as trypsins 1 and 2, were purified from the pancreas of the eel anguilla japonica by deae-cellulose column chromatography and sephadex g-75 gel filtration. the final preparation of trypsin 1 was homogeneous but that of trypsin 2 still contained impurities. both enzymes had similar ph optima of near 8.3 for the hydrolysis of n-tosyl-l-arginine methyl ester. trypsin 1 was stabilized by calcium ions but the stability of trypsin 2 was not affected by calcium ions. bot ...19853967490
characterization of an anionic trypsin from the eel (anguilla japonica).an enzyme, isolated from the pancreas of the eel anguilla japonica and designated as anionic trypsin 1, had a molecular weight of 26,000 and an isoelectric point of 5.5. the amino acid composition of the enzyme was similar to that of bovine cationic trypsin as well as anionic trypsins from other species of fish. the enzyme was stable at ph 6 to 9 in the presence of calcium ions. km and kcat values of the enzyme for n-tosyl-l-arginine methyl ester and n-tosyl-l-lysine methyl ester were quite simi ...19853967484
identification and synthesis of [1-asparagine, 5-valine, 9-glycine] angiotensin i produced from plasma of american eel anguilla rostrata.the major peptide produced by incubation of american eel (anguilla rostrata) plasma with the eel kidney extract was identified as [1-asparagine, 5-valine, 9-glycine] angiotensin i (i). two minor peptides were also identified as [1-aspartic acid, 5-valine, 9-glycine] angiotensin i (ii) and [5-valine, 9-glycine] angiotensin i-(3-10)-octapeptide (iii). these structures were further confirmed by comparison of these peptides on high-pressure liquid chromatography (hplc) and high-performance thin-laye ...19853979804
study on a special binding phenomenon of biliverdin in the blood of the eel, anguilla japonica.biliverdin is bound firmly with a plasma protein in the blood of the eel, anguilla japonica. the firm binding, although likely noncovalent, could be formed only in vivo under the experimental conditions. characterization of the carrier protein indicated that it is a serum albumin. a similar phenomenon is not detectable in ten other fish species examined in this study.19854028686
the histochemistry of urea-unmasked glycosaminoglycans in the skin of the eel, anguilla japonica.in the connective tissues of the dermis and subcutis of the eel skin, the histochemistry of urea-unmasked glycosaminoglycans has been studied by means of combined staining and enzyme digestion procedures. the staining procedures employed were alcian blue (ab) ph 1.0, ab ph 2.5, aldehyde fuchsin (af), periodic acid-schiff (pas), ab ph 2.5-pas, high iron diamine (hid) and low iron diamine (lid) methods, whereas the enzymes used were streptomyces and testicular hyaluronidases, chondroitinases abc a ...19852579932
sodium-retaining activity of chum salmon prolactin in some euryhaline teleosts.sodium-retaining activity of chum salmon prolactin (prl) was examined in several euryhaline teleosts. chum prl was 100 times more potent than ovine prl in maintaining plasma sodium levels in the hypophysectomized killifish, fundulus heteroclitus, transferred from 50% seawater to fresh water. the effects of prls were parabolic, high doses of the hormones being less effective than low doses. when injected into seawater-adapted fry of the ayu, plecoglossus altivelis, or into juvenile rainbow trout, ...19863781235
cardiovascular, respiratory, and blood adjustments to hypoxia in the japanese eel,anguilla japonica.the cardioventilatory performance of the japanese eel,anguilla japonica, was evaluated during acute exposure to hypoxia. the eel became an oxygen conformer as ambient po2 fell below the critical value of 110 mmhg. although arterial and venous po2 also fell progressively, the arterial o2 content remained constant down to an ambient po2 of about 60 mmhg. arterial blood o2 saturation was maintained at 85% even at 40 mmhg. the increase in the supply of o2 to the animal during hypoxia was due to a co ...198624233180
a comparative study on the binding characteristics of the tight serum biliverdin-protein complexes in two fishes: anguilla japonica and clinocottus analis.the binding characteristics of biliverdin to its carrier protein in the blue-green blood of clinocottus analis (woolly sculpin) and anguilla japonica (freshwater eel) were operationally defined by various chemical probing methods and spectra analysis. the biliverdin in c. analis is a rotatable coiled molecule enveloped in a hydrophobic pocket of its carrier protein. the biliverdin in a. japonica is an open form molecule with external hydrogen bond or weak ester bond interacting with the carrier ...19863743032
primary structure of eel (anguilla japonica) growth hormone.two molecular forms of growth hormone, ghs i and ii, were isolated from a culture medium of the eel pituitary (anguilla japonica). the complete amino acid sequence of gh i was determined in the present study. the hormone was reduced, carboxymethylated, and subsequently cleaved with cyanogen bromide and enzymes. intact eel gh i was also digested with enzyme. the resulting fragments were separated by reverse-phase high-performance liquid chromatography (hplc) and subjected to sequence analysis by ...19873609715
isolation of two forms of growth hormone secreted from eel pituitaries in vitro.two forms of growth hormone (gh) were purified by chromatofocusing of medium from cultured japanese eel (anguilla japonica) pituitaries. the pituitaries were organ-cultured in eagle's minimum essential medium with earle's salts. following polyacrylamide gel electrophoresis of the medium at ph 9.5, two prominent bands were seen with rf 0.36 and 0.29; they were designated as eghi and eghii, respectively. seven-hundred fifty milliliters of medium, in which 260 pituitaries were cultured for 6-10 wee ...19873549441
volume-sensitive taurine transport in fish erythrocytes.taurine plays an important role in cell volume regulation in both vertebrates and invertebrates. erythrocytes from two euryhaline fish species, the eel (anguilla japonica) and the starry flounder (platichthys stellatus) were found to contain high intracellular concentrations of this amino acid (approximately equal to 30 mmol per liter of cell water). kinetic studies established that the cells possessed a saturable high-affinity na+-dependent beta-amino-acid transport system which also required c ...19873585985
molecular cloning of eel growth hormone cdna and its expression in escherichia coli.cdna clones coding for growth hormone (egh) of japanese eel (anguilla japonica) have been isolated from a cdna library prepared from pituitary gland poly(a)+ rna. the nucleotide sequence of the egh cdna was determined. it codes for the prehormone of 209 amino acids (aa) including a putative signal peptide of 19 aa. the deduced amino acid sequence was identical with that determined for egh protein. the primary structure of egh was compared with those of other species growth hormones (chum salmon, ...19882468582
birefringent spores differentiate encephalitozoon and other microsporidia from coccidia.tissue sections containing protozoa with birefringent spores indicate an infection by microsporidia. hematoxylin and eosin (he) does not affect spore birefringence, but some special stains (goodpasture, brown and brenn, or gram) obscure it. encephalitozoon cuniculi from an infected puppy, glugea stephani from the winter flounder pseudopleuronectes americanus, and plistophora sp. from the japanese eel anguilla japonica all have birefringent spores. encephalitozoon was studied first and then the t ...19883134761
daily cycles in plasma melatonin levels under long or short photoperiod in the common carp, cyprinus carpio.a radioimmunoassay (ria) for plasma melatonin (mlt) was simplified for use with the common carp (cyprinus carpio). plasma was partially purified with sep-pak c18 cartridge before ria. the inhibition curves for the sep-pak c18 fraction from the plasma of carp, goldfish (carassius auratus), yellow tail (seriola quinqueradiata), japanese eel (anguilla japonica), bora (mugil cephalus cephalus), kisu (sillago japonica), ishigarei (kareius bicoloratus), and shimaisaki (rhyncopelates oxyrhynchus) were ...19883197949
occurrence of bilirubin-ix beta in the gallbladder bile of eel, anguilla japonica.bilirubin-ix beta isomer was detected in the bile of eel, anguilla japonica, as evidenced by high-performance liquid chromatography (hplc) analysis of free acid and its ethyl anthranilate diazo derivatives. bilirubin-ix beta accounted for 16.7 +/- 6.0 (mean +/- s.d.) percent of the total bilirubins in the bile of 40 eels analysed, and the percentage was much higher than that reported in the biles of mammals.19892804119
evidences for the presence of a somatomedin-like plasma factor(s) in the japanese eel, anguilla japonica.the indirect action of growth hormone (gh) through a somatomedin-like plasma factor(s) on the synthesis of mucopolysaccharides in the ceratobranchial cartilages of the japanese eel, anguilla japonica, was studied by monitoring the uptake of [35s]sulfate in vitro. addition of plasma obtained from intact eels significantly stimulated the sulfate uptake by the cartilages of hypophysectomized eel, whereas plasma from hypophysectomized eels had no effect. on the other hand, the plasma from hypophysec ...19902391031
effects of recombinant eel growth hormone on the uptake of [35s]sulfate by ceratobranchial cartilages of the japanese eel, anguilla japonica.effects of growth hormone (gh) on the synthesis of mucopolysaccharide by ceratobranchial cartilages of the japanese eel, anguilla japonica, were examined by monitoring the in vitro uptake of [35s]sulfate. the [35s]sulfate uptake decreased rapidly to one-third of the initial level during the first 3 days after hypophysectomy, and decreased gradually thereafter. when hypophysectomized eels were injected intramuscularly with recombinant eel gh (2 micrograms/g), the plasma gh concentrations increase ...19902391030
growth hormone secretion during longterm incubation of the pituitary of the japanese eel, anguilla japonica.growth hormone (gh) secretion from organ-cultured pituitaries of the eel (anguilla japonica) was studied during incubation in a defined medium for 2 weeks, using a homologous radioimmunoassay which does not distinguish between the two molecular forms of eel gh. the total amount of gh secreted increased gradually during the incubation period; so that the amount of gh released on day 14 was about 30 times greater than that on day 1. on day 14, the proportion of gh released relative to the total am ...199024221949
glucose transport in fish erythrocytes: variable cytochalasin-b-sensitive hexose transport activity in the common eel (anguilla japonica) and transport deficiency in the paddyfield eel (monopterus albus) and rainbow trout (salmo gairdneri).erythrocytes from individual common eels (anguilla japonica temminck and schlegel) exhibited widely variable initial rates of cytochalasin-b-sensitive 3-o-methyl-d-glucose (3-omg) zero-trans influx, in the range of 0-19.5 mmol l-cells-1 h-1 (5 mmol l-1 extracellular concentration at 20 degrees c, 50 animals tested). storage of cells at 4 degrees c in a glucose-containing medium for up to 72 h had no effect on 3-omg uptake, and there was no correlation between the sugar permeabilities of erythroc ...19902307927
ultrastructural and cytochemical study of elastic fibers in the ventral aorta of a teleost, anguilla japonica.previous studies have revealed that amorphous elastin and microfibrils are structural entities of mammalian elastic fibers. elastin shows a wide phylogenetic distribution, but the presence of elastin-associated microfibrils has not been demonstrated in teleost aorta. thus, we have ultrastructurally and cytochemically examined elastic fibers in the ventral aorta of eel, a teleost, by utilizing routine uranyl acetate and lead double staining, the tannic acid (ph 7.0)-uranyl acetate (ta-ua) method, ...19902297080
purification and characterization of bilirubin udp-glucuronyltransferase from the liver of eel, anguilla japonica.1. the enzyme bilirubin uridine diphosphate glucuronyltransferase (udpgt) was purified and characterized from the liver of eel, anguilla japonica. 2. the molecular weight of the enzyme was 88,000. 3. the optimal working ph of the enzyme was 7.5-8.0. the optimal working temperature of the enzyme was around 46 degrees c. 4. the km of bilirubin and uridine diphosphate glucuronic acid for this enzyme was 1.6 mm and 1.8 mm respectively. 5. the concentration of bilirubin did not show any significant e ...19902109666
hormonal induction of all stages of spermatogenesis in vitro in the male japanese eel (anguilla japonica).the importance of gonadotropins and androgens for spermatogenesis is generally accepted in vertebrates, but the role played by specific hormones has not been clarified. under cultivation conditions, male japanese eels (anguilla japonica) have immature testes containing only premitotic spermatogonia, type a and early-type b spermatogonia. in the present study, a recently developed organ-culture system for eel testes was used to determine in vitro effects of various steroid hormones on spermatogen ...19912062857
isolation and characterization of japanese eel prolactins.a highly purified prolactin (prl) was isolated from the pituitary of the japanese eel (anguilla japonica) by extraction with acid-acetone, gel filtration on sephadex g-75, and reversed-phase hplc on tsk-gel ods 120t and on tsk-gel tms 250. eel prl is comprised of two variants (eprl i and ii), which were separated by hplc on an ods column. the two prls were also secreted by organ-cultured pituitaries in a defined medium. after being dialyzed against distilled water and lyophilized, the medium was ...19912055439
presence of prolactin receptors in eel liver and carp kidney and growth hormone receptor in eel liver.1. 125i-labelled ovine prolactin and bovine growth hormone were used to test for the presence of prolactin and growth hormone receptors in membrane prepared from tissues of the white eel anguilla japonica, the carp ctenopharynogodon idellus and the ricefield eel monopterus albus. 2. high levels of specific 125i-labelled ovine prolactin binding were found in white eel liver membranes and carp kidney membranes. 3. high levels of specific 125i-labelled bovine growth hormone binding were detected in ...19911678335
c-reactive protein in eel: purification and agglutinating activity.c-reactive protein was highly purified from japanese eel (anguilla japonica) serum by precipitation with phosphatidyl-choline and ca2+. on sds-page, eel c-reactive protein (ecrp) migrated as a single band with a molecular weight of 24,000 under reducing and 23,500 under non-reducing conditions. the molecular weight of native ecrp was estimated to be 120,000 by sephacryl s-300 gel filtration. the ecrp was detected within the albumin region on immunoelectrophoresis. the ecrp showed an agglutinatin ...19911900197
development of a homologous radioimmunoassay for eel prolactin.a highly specific and homologous radioimmunoassay (ria) for the measurement of prolactin (prl) in the plasma and the pituitary of the eel was developed using a rabbit antiserum to eel prl. prl was purified from the pituitary of japanese eel (anguilla japonica). pituitary extracts and plasma from the japanese and european eels exhibited displacement curves parallel to the eel prl standard. plasma and pituitary extracts from chum salmon, rainbow trout, japanese charr, tilapia, goldfish, and carp, ...19912055440
human chorionic gonadotropin induces all stages of spermatogenesis in vitro in the male japanese eel (anguilla japonica).in the cultivated male japanese eel, spermatogonia are the only germ cells present in the testis. using a newly developed organ culture system, we obtained evidence that human chorionic gonadotropin (hcg) can induce the entire process of spermatogenesis, in vitro, from spermatogonia to spermatozoa within 24 days. the hcg-induced spermatogenesis in vitro was accompanied by a marked activation of sertoli cells and leydig cells, occurring prior to the beginning of spermatogonial proliferation. thes ...19912060708
nephroblastomas in the japanese eel, anguilla japonica temminck and schlegel.nephroblastomas were observed in 50 japanese eel reared at a farm for 5 to 9 months from 1989 after collection in the wild. the tumors, arising from the trunk kidney near the anus, were noted externally as abdominal swellings and varied in size from 30 to 75 mm in maximum diameter. most were elastic, solid, and well encapsulated. histologically, the nephroblastomas were composed of combinations of three main tissue elements. spindle- or oval-shaped cells resembling human blastema cells were obse ...19921314699
effects of insulin-like growth factor-i and insulin on the in-vitro uptake of sulphate by eel branchial cartilage: evidence for the presence of independent hepatic and pancreatic sulphation factors.the possible roles of insulin-like growth factor-i (igf-i) and insulin in regulating cartilage growth were studied in the teleost anguilla japonica. significant sulphation activity was found in the extracts of pancreas, liver and muscle, but not in those of kidney, intestine or spleen. the hepatic sulphation activity was significantly decreased by hypophysectomy or by fasting for 14 days, suggesting that this activity is regulated by pituitary function and nutritional status. northern blot analy ...19921613424
growth hormone (gh)-dependent hepatic gh receptors in the japanese eel, anguilla japonica: effects of hypophysectomy and gh injection.control mechanisms of growth hormone (gh) receptors in the eel liver were examined by following the time course of changes in total (mgcl2-treated membranes) and free (untreated membranes) gh-binding sites after hypophysectomy and replacement therapy with homologous gh. both total and free binding sites decreased significantly 1 week after hypophysectomy. scatchard analysis indicated that the reduction in gh receptors was primarily due to a decrease in the number of binding sites, rather than to ...19921577242
involvement of growth hormone and prolactin in the induction of vitellogenin synthesis in primary hepatocyte culture in the eel, anguilla japonica.multihormonal effects of bovine growth hormone (gh) and ovine prolactin (prl) on vitellogenin (vg) synthesis in response to estradiol-17 beta (e2) were electrophoretically examined in primary hepatocyte culture in the eel, anguilla japonica. newly synthesized protein after hormonal treatment was identified as vg by immunoblot and immunoelectrophoresis. a single injection of e2 into hypophysectomized eels failed in the induction of vg synthesis. similarly, e2 alone was insufficient to induce vg s ...19948138119
population structure of the japanese eel, anguilla japonica.mitochondrial dna (mtdna) sequences that include (a) a part of the cytochrome b gene, (b) two trna genes, and (c) a part of the noncoding d-loop region of 31 anguilla japonica (japanese eel) and 1 a. marmorata collected from taiwan, japan, and mainland china were determined to evaluate the population structure of japanese eel. among 30 genotypes identified from the 31 japanese eel mtdnas sequenced, there are 58 variable sites, predominantly clustered at the d-loop region. the phylogenetic tree c ...19948170366
the onset of spermatogenesis in fish.under cultivation conditions, male japanese eels (anguilla japonica) have immature testes containing only spermatogonia together with inactive testicular somatic cells, leydig cells and sertoli cells. using a recently developed organ culture system for eel testes, we have shown that hormonal induction of spermatogenesis in eel testes involves gonadotropin stimulation of leydig cells to produce 11-ketotestosterone, a potent androgen in fish. in turn, 11-ketotestosterone activates sertoli cells to ...19947835154
tocopherol levels in the plasma lipoproteins from japanese eel anguilla japonica.the alpha- and gamma-tocopherol levels in the plasma of japanese eel (anguilla japonica) were 53.9 micrograms and 1.3 micrograms/ml of plasma, respectively. the alpha-tocopherol in the plasma was mainly distributed as very-low-density lipoprotein and low-density lipoprotein (ldl), while ldl and high-density lipoprotein constituted most of the gamma-tocopherol. a highly positive coefficient of correlation was observed between the alpha-tocopherol and lipoprotein contents in japanese eel plasma.19958611757
spinal and parachordal neurons in the eel leptocephali, anguilla japonica revealed by labeling with a fluorescent dye.the spinal and parachordal neurons innervating the trunk muscles and skin in the japanese eel leptocephali, anguilla japonica, were retrogradely labeled with a fluorescent dye, dii (1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate). the spinal motoneurons innervating the trunk muscles were located in the medioventral part of the spinal cord and were divided into two populations on the basis of their soma sizes and position. the sensory neurons were labeled in the well-developed ...19958584211
[studies on the crude drugs used for the folk medicine described in "mimi-bukuro"]."mimi-bukuro" is a book written by moriyasu negishi in the edo period. m. negishi (1737-1815) was a magistrate in the town of edo. he was very much interested in listening and recording many kinds of stories, which were told by the various kinds of people, such as public officers, samurais, merchants, doctors, etc. among the stories of this book, some stories were found concerning folk medicine, medicinal substance and charms. in this report, i studied the medicines which were used in these stor ...199511613523
a highly repetitive sequence isolated from genomic dna of the medaka (oryzias latipes).a highly repetitive interspersed dna sequence (mre) was isolated from the genome of medaka, oryzias latipes, and characterized. three distinct sequences of mre were cloned and compared. the conserved sequences of mre were approximately 220 bp in length. on average, one copy of mre was present in every 153 kb, and the number of copies of mre in the genome was calculated to be approximately 9800. mre constituted approximately 0.14% of the genome. mre-related sequences were not detected in carp (cy ...19968817927
fish testicular 11beta-hydroxylase: cdna cloning and mrna expression during spermatogenesis.we isolated and characterized a cdna encoding testicular 11beta-hydroxylase, cytochrome p450(11beta) from the japanese eel (anguilla japonica) testis. the cdna contains an open reading frame that encodes a protein of 511 amino acids. the predicted amino acid sequence shares 38-48% homology with those of adrenal p450(11beta) from mammals and frog. transient expression in cos 1 cells confirmed that the protein encoded by this cdna had p450(11beta) activity. northern blotting revealed a single 1.8 ...19968955357
a unique lipoprotein profile found in the plasma of cultured japanese eelanguilla japonica: very low density lipoprotein, but not high density lipoprotein, is the main component of plasma.a unique lipoprotein profile was found in the plasma of cultured japanese eel,anguilla japonica which accumulated more lipid in its muscle than in its liver. the plasma lipoprotein level of japanese eels was in excess of 54 mg ml(1), a concentration considered to be hyperlipoproteinemic in relation to other teleosts. the plasma lipoproteins consisted of very low density lipoprotein (vldl, density (d)<1.006 g ml(1)) low density lipoprotein (ldl, 1.006<d<1.085 g ml(1)), high density lipoprotein 2 ...199624194356
changes in plasma atrial and ventricular natriuretic peptide concentrations after transfer of eels from freshwater to seawater or vice versa.changes in plasma atrial and ventricular natriuretic peptide (anp and vnp) concentrations were examined in conscious eels, anguilla japonica, after transfer from freshwater (fw) to seawater (sw) or vice versa. these changes were compared in ventral and dorsal aortic blood since clearance receptors are present in the gills. plasma na concentration and osmolality in the ventral aortic blood rose linearly after transfer of fw eels to sw. six hours after transfer, plasma anp levels increased from 28 ...19968954767
whole body autoradiography and microautoradiography in eels after intra-arterial administration of 125i-labeled eel anp.125i-labeled eel atrial natriuretic peptide (anp) was administered into the ventral or dorsal aorta of freshwater (fw) and seawater (sw) eels, anguilla japonica, and the major target organs were explored by whole body autoradiography. localization of the anp binding in the target organs was also examined at tissue and cell levels by microautoradiography using tissue sections. whole body autoradiography revealed that the specific label was accumulated predominantly in the gill, with lesser amount ...19968897983
osmotic and volaemic regulation of atrial and ventricular natriuretic peptide secretion in conscious eels.the effects of acute manipulation of plasma osmolality and blood volume on plasma atrial and ventricular natriuretic peptide (anp and vnp) levels were examined in conscious freshwater eels, anguilla japonica. a bolus injection of hypertonic nacl (0.85 m and 1.7 m, 2.5 ml/kg body weight) through a catheter into the ventral aorta produced increases in plasma na concentration and osmolality with parallel concentration-dependent, transient increases in plasma anp and vnp levels. plasma anp and vnp l ...19968691102
molecular cloning and characterization of japanese eel estrogen receptor cdna.a cdna encoding the japanese eel, anguilla japonica, estrogen receptor (er) was isolated and sequenced. this cdna contains a complete open reading frame encoding 573 amino acid residues, and the molecular weight of this protein is calculated to be 63417. the amino acid sequence of the eel er shows high homology of the dna binding (80%) and ligand binding (55%) domains with those of other species. however, the other domains show greatly reduced homology (10-20%). when the cdna was ligated to the ...19968793852
molecular cloning of the cdnas encoding pituitary glycoprotein hormone alpha- and gonadotropin ii beta-subunits of the japanese eel, anguilla japonica, and increase in their mrnas during ovarian development induced by injection of chum salmon pituitary homogenate.cdnas encoding the glycoprotein hormone alpha- and gonadotropin (gth) ii beta-subunits of japanese eel (anguilla japonica) pituitary were cloned using the polymerase chain reaction. the nucleotide sequence of the glycoprotein hormone alpha-subunit cdna was 364 base pairs (bp) long, encoding 117 amino acids, and that of the gth ii beta-subunit cdna was 433 bp long, encoding 140 amino acids. the deduced amino acid sequence of each mature subunit showed high homology with those of other teleosts, i ...19969156520
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