Publications

linkage relations of the sex-linked characters in drosophila obscura.		1922	17245985
a new sex-ratio abnormality in drosophila obscura.		1928	17246563
[studies on chromosomal structure of european species of drosophila obscura group].		1953	13137262
[biometric comparison of two populations of drosophila obscura].		1959	13660247
courtship behaviour in the drosophila obscura group. ii. comparative studies.		1965	5824950
genetic variation in natural populations of drosophila obscura.		1971	5136539
genetic distance and evolutionary relationships in the drosophila obscura group.		1972	28555739
a comment on evolution in the drosophila obscura species group.		1974	28563026
a reply to dr. farris' comment on evolution in the drosophila obscura species group.		1974	28563022
the relationships of drosophila obscura fallén and d. subobscura collin to naturally-occurring fruits.	the relation between british woodland drosophila and naturally-occurring fruit was examined in three ways: (1) field observations show d. subobscura emerging in large numbers, and d. obscura and d. ambigua in much smaller numbers, from rowan and woody nightshade, but not other fruits. d. subobscura used rowan inefficiently; its peak of emergence is in mid-november. (2) microbial studies suggest that adult flies do not feed on naturally-occurring fruit in the wild, and that laboratory-rotted frui ...	1975	28308991
temporal variations in the reproductive condition of drosophila obscura fallén and d. subobscura collin.	the reproductive condition, insemination status and fat content of drosophila obscura and d. subobscura were investigated in samples from natural populations, and in near-natural and artificial experimental situations. in northern england d. obscura populations pass through either three of four generations per year, one of which includes a period of adult diapause which involves both reproductive condition and fat content. appropriate day lengths lead to both the initiation and the breaking of t ...	1976	28309211
genetic distance and evolutionary relationships in the drosophila obscura group.		1983	28568133
dispersal rates of species of the drosophila obscura group: implications for population structure.		1984	28563787
puff activity after heat shock in two species of the drosophila obscura group.	when individuals of drosophila guanche are submitted to heat shock, five new puffs are induced. these puffs usually do not appear during normal development. comparing these results with those obtained in drosophila subobscura, also belonging to the obscura group, differences between the induced puffing pattern of both species have been found.	1987	3691744
mitochondrial dna evolution in the drosophila obscura group.	we report a restriction-site study of the mitochondrial dna (mtdna) of seven species of the drosophila obscura group. one species (d. azteca) belongs to the affinis subgroup; the other six species are classified in the obscura subgroup, three of them being from the old-world species (d. obscura, d. ambigua, and d. subobscura) and three from the new-world species (d. pseudoobscura, d. persimilis, and d. miranda). the mtdna patterns suggest that the phylogeny of the group needs to be revised. the ...	1988	3221793
"sex ratio" meiotic drive in drosophila testacea.	we document the occurrence of "sex ratio" meiotic drive in natural populations of drosophila testacea. "sex ratio" males sire greater than 95% female offspring. genetic analysis reveals that this effect is due to a meiotically driven x chromosome, as in other species of drosophila in which "sex ratio" has been found. in contrast to other drosophilids, the "sex ratio" and standard chromosomes of d. testacea do not differ in gene arrangement, implying that the effect may be due to a single genetic ...	1990	2249763
the phylogeny of nine species of the drosophila obscura group inferred by the banding homologies of chromosomal regions. ii. element e.	the phylogenetic relationships among nine species of drosophila belonging to the obscura group were investigated by establishing (according to their banding similarities) the homologous chromosome segments of element e (equivalent to chromosome o of d. subobscura). the phylogenetic relationships were based on the existence of segments in different triads of species, which could only be produced by overlapping inversions. this permitted the ordering of the species belonging to each triad. drosoph ...	1990	2079439
evolutionary implications of dna divergence in the drosophila obscura group.	using dna-dna hybridization, we have determined the degree of single-copy dna (scdna) divergence among eight species of the drosophila obscura group. these include old world and new world species as well as members of two subgroups. contrary to classical systematics, members of the affinis subgroup are more closely related to american members of the obscura subgroup than are old world species. the old world species are not a monophyletic group. the degree of scdna divergence among species is not ...	1990	28564311
mitochondrial dna evolution in the obscura species subgroup of drosophila.	mitochondrial dna (mtdna) restriction site maps for nine species of the drosophila obscura subgroup and for drosophila melanogaster were established. taking into account all restriction enzymes (12) and strains (45) analyzed, a total of 105 different sites were detected, which corresponds to a sample of 3.49% of the mtdna genome. based on nucleotide divergences, two phylogenetic trees were constructed assuming either constant or variable rates of evolution. both methods led to the same relations ...	1990	2120450
the phylogeny of nine species of the drosophila obscura group inferred by the banding homologies of chromosomal regions. i. element b.	the phylogenetic relationships among nine drosophila species belonging to the obscura group were investigated by establishing the segments displaying banding homologies in their element b (equivalent to the u element of d. subobscura). the phylogenetic ordering of the species was accomplished using overlapping inversions. two african species, d. kitumensis and d. microlabis, were investigated. these species are homosequential for their element b gene arrangement but differ from that of d. obscur ...	1991	1889738
comparative kinetics of short and long sperm in sperm dimorphic drosophila species.	all species of the drosophila obscura group exhibit within-ejaculate sperm length dimorphism. the present work is a contribution to the understanding of sperm competition through a comparative study of sperm kinetic parameters in four of these species. videomicrographic observations at 200 frames per second of sperm from males and females, out of the storage organ, prior or after storage were made. drosophila sperm display both major and minor waves. the former is analysed by measuring coiling d ...	1991	1934086
in situ localization of the antennapedia gene on the chromosomes of nine drosophila species of the obscura group.	the homeotic antennapedia gene, cloned from the genomic dna of d. subobscura, was localized on the polytene chromosomes of nine species of the drosophila obscura group. in all of them, the probe used hybridized on chromosomes equivalent to the e element of müller's terminology. these results are consistent with the idea that single copy genes do not move around the genome and that chromosomal elements have conserved their genetic identity during evolution.	1991	1917542
the phylogeny of nine species of the drosophila obscura group inferred by the banding homologies of chromosomal regions. iii. element d.	the phylogenetic relationships among nine species belonging to the obscura group of the genus drosophila were deduced, based on similarities of the banding pattern of their polytene chromosomal element d. these similarities were inferred by the comparison of chromosomal photomaps. the phylogenetic reconstruction was the most parsimonious based on seriation by overlapping inversions and on the principle of conservation/disassociation of nearby located segments. the gene sequences of element d for ...	1992	1473723
phylogenetic reconstruction of the drosophila obscura group, on the basis of mitochondrial dna.	we have constructed restriction-site maps of the mtdnas in 13 species and one subspecies of the drosophila obscura group. the traditional division of this group into two subgroups (affinis and obscura) does not correspond to the phylogeny of the group, which shows two well-defined clusters (the nearctic affinis and pseudoobscura subgroups) plus a very heterogeneous set of anciently diverged species (the palearctic obscura subgroup). the mtdna of drosophila exhibits a tendency to evolve toward hi ...	1992	1630303
a duplication including the y allele of lcp2 and the trim retrotransposon at the lcp locus on the degenerating neo-y chromosome of drosophila miranda: molecular structure and mechanisms by which it may have arisen.	evolutionary changes during the process of sex chromosome differentiation in drosophila miranda are associated with massive dna rearrangements. comparing the dna structure of the larval cuticle protein (lcp) region from the x2 and neo-y chromosome pair, we observed insertions, deletions and a large duplication at the neo-y chromosomal locus. the duplication encompasses a complete copy of the neo-y allele of lcp2, and the isy3 and the isy4 insertion sequences. the latter was identified as a retro ...	1993	8392013
relationships in the drosophila obscura species group, inferred from mitochondrial cytochrome oxidase ii sequences.	we compare the sequences for the mitochondrial cytochrome oxidase ii gene of 13 species of the drosophila obscura group. the survey includes six members of the d. affinis subgroup, four of the d. pseudoobscura subgroup, and three of the d. obscura subgroup. in all species, the gene is 688 nucleotides in length, encoding a protein of 229 amino acids plus the first position t of the stop codon. the sequences show the typical high-transition bias for closely related species, but that bias is essent ...	1993	8393127
gradual evolution of a specific satellite dna family in drosophila ambigua, d. tristis, and d. obscura.	the highly repetitive satellite dna family "atoc180" is specific for the three closely related species drosophila obscura, d. ambigua, and d. tristis but does not occur in their closest relatives d. subsilvestris and d. bifasciata. approximately 10,000 copies/haploid genome of approximately 180-bp repetition units are tandemly arranged in the centromeric heterochromatin of all chromosomes of all three species. molecular analysis of 29 cloned repeats shows much intra- and interspecific sequence h ...	1993	8336547
characterization of the length polymorphism in the a + t-rich region of the drosophila obscura group species.	in the twelve drosophila obscura group species studied, belonging to the affinis, obscura, and pseudoobscura subgroups, the mitochondrial dna length ranges from 15.8 to 17.2 kb. this length polymorphism is mainly due to insertions/deletions in the variable region of the a + t-rich region. in addition, one species (d. tristis) possess a tandem duplication of a 470-bp fragment that contains the replication origin. the same duplication has occurred at least twice in the drosophila evolutionary hist ...	1993	8483159
the phylogeny of nine species of the drosophila obscura group inferred by the banding homologies of the chromosomal regions. iv. element c.	homologies of the sections of the polytene chromosomes of element c, among several species of the obscura group of the genus drosophila, were established according to the similarity of their banding patterns. the information gathered was used to construct an unrooted phylogenetic tree based on qualitative criteria. this tree is compared to three other similar trees derived from independent information provided by the study of chromosomal elements b, d and e. the general congruence of the pattern ...	1993	8454475
functional nonequivalence of sperm in drosophila pseudoobscura.	we report on a form of sperm polymorphism, termed polymegaly, that occurs in species of the drosophila obscura group. individual males of species in this group characteristically produce more than one discrete length of nucleated, motile sperm. hypotheses suggested to explain the evolutionary significance of sperm polymorphism have been either nonadaptive or adaptive, with the latter focusing on sperm competition or nutrient provisioning. these hypotheses assume all sperm types fertilize eggs; h ...	1994	7972038
phylogeny of the drosophila obscura species group deduced from mitochondrial dna sequences.	approximately 2 kb corresponding to different regions of the mtdna of 14 different species of the obscura group of drosophila have been sequenced. in spite of the uncertainties arising in the phylogenetic reconstruction due to a restrictive selection toward a high mtdna a+t content, all the phylogenetic analysis carried out clearly indicate that the obscura group is formed by, at least, four well-defined lineages that would have appeared as the consequence of a rapid phyletic radiation. two of t ...	1994	7528808
a new p element subfamily from drosophila tristis, d. ambigua, and d. obscura.	a new p element subfamily, designated t-type, was found in the genomes of the three closely related species drosophila ambigua, drosophila obscura, and drosophila tristis. the subfamily comprises both full-sized and internally deleted p elements. the t-type element of d. ambigua is longer than the canonical p elements owing to a 300-bp insertion in the 3' noncoding region. tandemly arranged t-type elements were detected in d. ambigua and d. tristis. the overall structure of t-type elements resem ...	1996	8890523
characterization and molecular analysis of adh retrosequences in species of the drosophila obscura group.	retrosequences, genes, and pseudogenes originated by retrotranscription are frequent components of vertebrate genomes, but they have only occasionally been described in invertebrates. in drosophila, very few retrosequences have been reported, among them those of alcohol dehydrogenase (adh) and phosphoglyceromutase (pglym). although 52 adh gene sequences are available for comparison, adh retrosequences have been described only in the sibling species d. teissieri and d. yakuba (melanogaster subgro ...	1997	9402741
p element domestication: a stationary truncated p element may encode a 66-kda repressor-like protein in the drosophila montium species subgroup.	functional p transposable elements can be separated into two distinct classes: mobile elements, which present the canonical structure, with transposase and repressor functions, and immobile p sequences truncated in 5' and 3' by loss of the terminal inverted repeats and exon 3, which retain only the repressor function. this second class was first described in some species of the drosophila obscura group. here, we describe a new truncated immobile p sequence cloned from one species of the drosophi ...	1997	9364771
evolution of the drosophila obscura species group inferred from the gpdh and sod genes.	the drosophila obscura group consists of several dozen nearctic and palearctic species, with a phylogeny that remains largely unresolved in spite of numerous morphological, cytogenetic, and molecular investigations. we have partially sequenced two genes, gpdh (about 1000 bp) and sod (about 700 bp) in 12-13 species and 1 subspecies in order to settle the issues. difficulties in resolving the phylogeny emanate from the rapid sequence of successive radiations. nevertheless, the following conclusion ...	1997	9007023
is the production of multiple sperm types adaptive?	males of many species concurrently produce more than one sperm type, now called sperm heteromorphism. in the drosophila obscura group, all species examined to date produce multiple sperm types that differ in sperm length. short sperm types in at least three obscura group species do not participate in fertilization, leading to questions regarding the adaptive value of sperm heteromorphism. the common and pervasive inheritance of this trait in the obscura group, however, may indicate that sperm he ...	1997	28568578
mitochondrial dna phylogenies for the drosophila obscura group.	species belonging to the obscura group of the genus drosophila have long held a central position in evolutionary studies, especially in experimental population genetics. despite the considerable amount of accumulated knowledge, many of the phylogenetic relationships of the species in the group remain unclear. here we present dna sequence data for the mitochondrial gene cytochrome oxidase i (coi) for 13 species native to both the old and new worlds. we combine these data with seven other mitochon ...	1997	28565356
the risk of sperm competition and the evolution of sperm heteromorphism.	members of the drosophila obscura species group exhibit sperm heteromorphism in which males simultaneously produce two different morphologies of sperm, short and long. short sperm represent at least 50% of the ejaculate in several species but do not function in fertilization and thus, the evolutionary significance of this phenomenon is unknown. selective pressures associated with the risk of sperm competition have been suggested to explain the maintenance of sperm heteromorphism. here i test: (1 ...	1998	9933548
molecular evolution of p transposable elements in the genus drosophila. ii. the obscura species group.	a phylogenetic analysis of p transposable elements in the drosophila obscura species group is described. multiple p sequences from each of 10 species were obtained using pcr primers that flank a conserved region of exon 2 of the transposase gene. in general, the p element phylogeny is congruent with the species phylogeny, indicating that the dominant mode of transmission has been vertical, from generation to generation. one manifestation of this is the distinction of p elements from the old worl ...	1998	9732455
only long sperm are fertilization-competent in six sperm-heteromorphic drosophila species.	males of many species exhibit sperm heteromorphism, in which multiple morphologies of sperm are produced in a common testis (see references in [1]). polymegaly, a form of sperm heteromorphism, is found in the drosophila obscura group and is characterized by the production of two size classes of nucleated sperm that differ only in head and tail lengths [1,2]. both the length and the ratio of sperm types produced is species-specific [1,3-5], and each sperm type develops in its own bundle deriving ...	1998	9501071
reevaluation of phylogeny in the drosophila obscura species group based on combined analysis of nucleotide sequences.	the drosophila obscura species group has served as an important model system in many evolutionary and population genetic studies. despite the amount of study this group has received, some phylogenetic relationships remain unclear. while individual analysis of different nuclear, mitochondrial, allozyme, restriction fragment, and morphological data partitions are able to discern relationships among closely related species, they are unable to resolve relationships among the five obscura species sub ...	1999	10381316
ancient and recent horizontal invasions of drosophilids by p elements.	p elements of two different subfamilies designated as m- and o-type are thought to have invaded host species in the drosophila obscura group via horizontal transmission from external sources. sequence comparisons with p elements isolated from other species suggested that the horizontal invasion by the o-type must have been a rather recent event, whereas the m-type invasion should have occurred in the more distant past. to trace the phylogenetic history of o-type elements, additional taxa were sc ...	2000	11116331
evolutionary dynamics of the sgm transposon family in the drosophila obscura species group.	sgm (drosophila subobscura, drosophila guanche, and drosophila madeirensis) transposons are a family of transposable elements (tes) in drosophila with some functional and structural similarities to miniature inverted-repeat transposable elements (mites). these elements were recently active in d. subobscura and d. madeirensis (1-2 mya), but in d. guanche (3-4 mya), they gave rise to a species-specifically amplified satellite dna making up approximately 10% of its genome. sgm elements were already ...	2000	11070048
evolution of gypsy endogenous retrovirus in the drosophila obscura species group.	the ty3/gypsy family of retroelements is closely related to retroviruses, and some of their members have an open reading frame resembling the retroviral gene env. sequences homologous to the gypsy element from drosophila melanogaster are widely distributed among drosophila species. in this work, we report a phylogenetic study based mainly on the analysis of the 5' region of the env gene from several species of the obscura group, and also from sequences already reported of d. melanogaster, drosop ...	2000	10908638
molecular evolution of the ocnus and janus genes in the drosophila melanogaster species subgroup.	genes involved in male fertility are potential targets for sexual selection, and their evolution may play a role in reproductive isolation and speciation. here we describe a new drosophila melanogaster gene, ocnus (ocn), that encodes a protein abundant in testes nuclear extracts. rt-pcr indicates that ocn transcription is limited to males and is specific to testes. ocn shares homology with another testis-specific gene, janusb (janb), and is located just distal to janb on chromosome 3. the two ge ...	2001	11319264
the actin loci in the genus drosophila: establishment of chromosomal homologies among five palearctic species of the drosophila obscura group by in situ hybridization.	chromosomal homologies among the four palearctic drosophila obscura group species d. ambigua, d. tristis, d. obscura, and d. subsilvestris and the "trans-palearctic" species d. bifasciata were established by in situ hybridization using the 5c actin gene of d. melanogaster as a probe. in all species two labeling sites were detected in each of chromosomal elements c and e and one in each of chromosomal elements a and d. in addition one labeling site was detected on element b for the species d. sub ...	2001	11862451
distribution of the bilbo non-ltr retrotransposon in drosophilidae and its evolution in the drosophila obscura species group.	the bilbo element is a non-ltr retrotransposon isolated from drosophila subobscura. we conducted a distribution survey by southern blot for 52 species of the family drosophilidae, mainly from the obscura and melanogaster groups. most of the analyzed species bear sequences homologous to bilbo from d. subobscura. in the obscura group, species from the same species subgroup also share similar southern blot patterns. to investigate the phylogenetic relationship among these elements, we analyzed eigh ...	2001	11264411
the actin loci in the genus drosophila: establishment of chromosomal homologies among five nearctic species of the drosophila obscura group by in situ hybridization.	the actin genes of five nearctic species of the drosophila obscura group were mapped by in situ hybridization, using the 5c actin gene of d. melanogaster as a probe. in all species but d. azteca eight actin loci were observed variously dispersed over all five (a- e) chromosomal elements. in d. azteca ten actin hybridization sites were found; four of which most probably originated by duplications or by transposition events. although the five nearctic species differ from all other drosophila speci ...	2002	12424525
recurrent exon shuffling between distant p-element families.	two independent stationary p-related neogenes had been previously described in the drosophila obscura species group and in the drosophila montium species subgroup. in drosophila melanogaster, p-transposable elements can encode an 87 kda transposase and a 66 kda repressor, but the p-neogenes have only conserved the capacity to encode a 66 kda repressor-like protein specified by the first three exons. we have previously analyzed the genomic modifications associated with the transition of a p-eleme ...	2003	12598685
the drosophila obscura species-group (diptera, drosophilidae) from yunnan province, southern china.	three new and two known species of the drosophila (sophophora) obscura species-group are reported from yunnan province, southern china. the sinobscura species-subgroup is newly established by d. sinobscura, d. hubeiensis and d. luguensis sp. nov. geographic distribution of the obscura group in and around china is discussed, and a key to 10 chinese species of the obscura group is provided.	2003	12832830
adaptive significance of amylase polymorphism in drosophila xiii. old world obscura species subgroup divergence according to biochemical properties of alpha-amylase.	biochemical properties of enzyme alpha-amylase were surveyed in drosophila obscura old world group of species (d. subobscura, d. ambigua, d. obscura and d. tristis) sampled in the same habitat, with the aim to reveal some ecological and evolutionary aspects of amylase polymorphism, which has been studied extensively in d. subobscura, but not compared with other species in the group. the data obtained show that d. subobscura is distinct from the other three species regarding all biochemical amyla ...	2003	12655135
domesticated p elements in the drosophila montium species subgroup have a new function related to a dna binding property.	molecular domestication of a transposable element is defined as its functional recruitment by the host genome. to date, two independent events of molecular domestication of the p transposable element have been described: in the drosophila obscura species group and in the drosophila montium species subgroup. these p neogenes consist of stationary, nonrepeated sequences, potentially encoding 66-kda repressor-like (rl) proteins. here we investigate the function of the montium p neogenes. we provide ...	2005	16132470
sexual size dimorphism in a drosophila clade, the d. obscura group.	the drosophila obscura clade consists of about 41 species, of which 20 were used for analyses of wing and thorax length. our primary goal was to investigate the magnitude of sexual size dimorphism (ssd) of these traits within this clade and to test rensch's rule [when females are larger than males, ssd (e.g., female/male ratio) should decrease with body size]. our secondary goal was methodological and involved evaluating for these flies alternative measures of ssd (female/male ratio, female/male ...	2006	16978850
molecular phylogeny of the drosophila obscura species group, with emphasis on the old world species.	species of the drosophila obscura species group (e.g., d. pseudoobscura, d. subobscura) have served as favorable models in evolutionary studies since the 1930's. despite numbers of studies conducted with varied types of data, the basal phylogeny in this group is still controversial, presumably owing to not only the hypothetical 'rapid radiation' history of this group, but also limited taxon sampling from the old world (esp. the oriental and afrotropical regions). here we reconstruct the phylogen ...	2007	17555574
inversion polymorphism in drosophila obscura.	the inversion polymorphism of drosophila obscura fallen, a european species of the obscura group of the subgenus sophophora, is described. a total of 21 inversions have been recorded; they are located in the five large chromosomes of the species (a dot chromosome is also present) and form 25 gene arrangements present in the species' natural populations. strains from five different countries were studied. two of these inversions were found to be pericentric, and the remaining were paracentric. th ...	2007	2013686
rhabdoviruses in two species of drosophila: vertical transmission and a recent sweep.	insects are host to a diverse range of vertically transmitted micro-organisms, but while their bacterial symbionts are well-studied, little is known about their vertically transmitted viruses. we have found that two sigma viruses (rhabdoviridae) recently discovered in drosophila affinis and drosophila obscura are both vertically transmitted. as is the case for the sigma virus of drosophila melanogaster, we find that both males and females can transmit these viruses to their offspring. males tran ...	2011	21339477
permanent genetic resources added to molecular ecology resources database 1 april 2013-31 may 2013.	this article documents the addition of 234 microsatellite marker loci to the molecular ecology resources database. loci were developed for the following species: acipenser sinensis, aleochara bilineata, aleochara bipustulata, barbus meridionalis, colossoma macropomum, delia radicum, drosophila nigrosparsa, fontainea picrosperma, helianthemum cinereum, liomys pictus, megabalanus azoricus, pelteobagrus vachelli, pleuragramma antarcticum, podarcis hispanica type 1a, sardinella brasiliensis and scle ...	2013	23937578
enhanced arbovirus surveillance with deep sequencing: identification of novel rhabdoviruses and bunyaviruses in australian mosquitoes.	viral metagenomics characterizes known and identifies unknown viruses based on sequence similarities to any previously sequenced viral genomes. a metagenomics approach was used to identify virus sequences in australian mosquitoes causing cytopathic effects in inoculated mammalian cell cultures. sequence comparisons revealed strains of liao ning virus (reovirus, seadornavirus), previously detected only in china, livestock-infecting stretch lagoon virus (reovirus, orbivirus), two novel dimarhabdov ...	2013	24314645
the life cycle of drosophila orphan genes.	orphans are genes restricted to a single phylogenetic lineage and emerge at high rates. while this predicts an accumulation of genes, the gene number has remained remarkably constant through evolution. this paradox has not yet been resolved. because orphan genes have been mainly analyzed over long evolutionary time scales, orphan loss has remained unexplored. here we study the patterns of orphan turnover among close relatives in the drosophila obscura group. we show that orphans are not only eme ...	2014	24554240
twenty-five new viruses associated with the drosophilidae (diptera).	drosophila melanogaster is an important laboratory model for studies of antiviral immunity in invertebrates, and drosophila species provide a valuable system to study virus host range and host switching. here, we use metagenomic rna sequencing of about 1600 adult flies to discover 25 new rna viruses associated with six different drosophilid hosts in the wild. we also provide a comprehensive listing of viruses previously reported from the drosophilidae. the new viruses include iflaviruses, rhabdo ...	2016	27375356
non-destructive species identification of drosophila obscura and d. subobscura (diptera) using near-infrared spectroscopy.	the vinegar flies drosophila subobscura and d. obscura frequently serve as study organisms for evolutionary biology. their high morphological similarity renders traditional species determination difficult, especially when living specimens for setting up laboratory populations need to be identified. here we test the usefulness of cuticular chemical profiles collected via the non-invasive method near-infrared spectroscopy for discriminating live individuals of the two species. we find a classifica ...	2017	22885252