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on the isolation, cultivation and classification of the so-called intracellular "symbiont" or "rickettsia" of periplaneta americana.in periplaneta americana, the large american roach, bacteriocytes are found in both sexes. these bacteriocytes are scattered throughout the fat tissue and their cytoplasm is filled with microorganisms. evidence is presented to show that the intracellular forms are diphtheroidal bacilli. these diphtheroids are transmitted from one generation to another through the ova. a method is described whereby tissues, free from contaminants, may be obtained from periplaneta. a medium and a new method, the " ...193019869679
survival of ingested salmonella in the cockroach periplaneta americana. 195212996751
the occurrence and movement of periplaneta americana (l.) within an urban sewerage system. 195413138840
[a new cervical organ in blatta orientalis (l.) and periplaneta americana (l.) producing a secretion fluorescent under wood light. (dictyopt. blattidae)]. 195414351495
[first data for characterization of the secretion of the latero-cervical organ of blatta orientalis (l.) and periplaneta americana (l.) (dictyopt. blattidae)]. 195414351496
[histological studies of toxoplasma-infected insects (calliphora erythrocephala, periplaneta americana)]. 195513371892
the spectral sensitivities of the dorsal ocelli of cockroaches and honeybees; an electrophysiological study.the spectral sensitivities of the dorsal ocelli of cockroaches (periplaneta americana, blaberus craniifer) and worker honeybees (apis mellifera) have been measured by electrophysiological methods. the relative numbers of quanta necessary to produce a constant size electrical response in the ocellus were measured at various wave lengths between 302 and 623 mmicro. the wave form of the electrical response (erg) of the dark-adapted roach ocellus depends on the intensity but not the wave length of t ...195813563806
the conjugated plasma proteins of the american cockroach. i. the normal state.five protein fractions have been separated by paper electrophoresis from the plasma of the american cockroach. with the utilization of various staining procedures several of the plasma fractions were shown to be conjugated proteins. two of these (fractions ii and iv) are readily identifiable by their phospholipid, carbohydrate, and protein composition. a third conjugated protein, fraction iii, is characterized by its high neutral lipid and sterol content. this lipoprotein is also sex-specific. a ...196014446367
stimulation of uterine contraction by extracts of the cockroach, periplaneta.blood and extracts of gut of the american cockroach, periplaneta americana, powerfully stimulate contractions of the isolated rat uterus. the contractions are characterized by long latency and a prolonged relaxation phase having superimposed rhythmic contractions. evidence indicates that the stimulating factor is not acetylcholine, 5-hydroxytryptamine, histamine, or substance p. the active factor is heat labile and nondialyzable.196117731378
a comparative study of the modes of action of fluoroacetamide and fluoroacetate in the mouse and american cockroach. 196314074120
[periplaneta americana, new experimental host of hymenolepis nana, fraterna variety]. 196514341954
the american cockroach, periplanta americana, as an intermediate host of the pentastomid, raillietiella hemidactyli. 19654221429
[a new gregarine, fusiona geusi, n. sp., n. gen., n. fam., n. superfam., from the south american cockroach pycnoscelus surinamensis l. 1758]. 19654960418
properties of bursicon: an insect protein hormone that controls cuticular tanning.on gel filtration the retention volume of bursicon indicates a molecular weight of about 40,000. on disc electrophoresis bursicon migrates toward the anode and appears at an r(f) (relative to the marker dye) of 0.3 to 0.4. the properties of fractions with bursicon activity in blood, brain, and ganglion of a fly (sarcophaga bullata), and in blood, ganglion, and corpora cardiaca of a roach (periplaneta americana) are similar, but not identical. bursicon in the blood is more heat labile, and the ac ...19665908970
lysozyme-like action of enzymes from the cockroach periplaneta americana and from some other sources. 19676076408
glyceryl ethers in insects: identification of alkyl and alk-1-enyl glyceryl ether phospholipids.alkyl and alk-1-enyl glyceryl ethers have been identified in the phospholipids of three insect species, the american cockroach (periplaneta americana), the tobacco budworm (heliothis virescens), and the boll weevil (anthonomus grandis). glyceryl ethers were not detected in the neutral lipids. the ethers were found in the phospholipid fraction of whole insects and in isolated fat body tissue. the ether content varied among the three insect species, and fluctuated during various developmental stag ...196817805805
the effect of water loss on the haemolymph of arenivaga sp. and periplaneta americana. 19685657192
lysozyme retention by cockroach periplaneta americana l.blood from the cockroach periplaneta americana usually does not react with a suspension of micrococcus lysodeikticus. when lysozyme is added to the mixture it causes an immediate reduction in the optical density at 450 nanometers which is soon followed by a strong rise. blood from roaches injected with lysozyme causes a similar series of changes in the optical density of a suspension of m. lysodeikticus, an indication that lysozyme has been retained in the blood. it may persist for many days or ...19695762765
[experimental studies on the action mechanism of neurohormone d from periplaneta americana (l) on the "biological model" of bufo bufo (l) urinary bladder]. 19695765445
heterogeneity in the fibre composition in the flight muscles of periplaneta americana and belostoma sp. 19705478651
[further experimental studies on the mechanism of action of neurohormone d from periplaneta americana (l) on the "biologic model" of bufo bufo urinary bladder: influencing of na+-transport]. 19714328748
l-leucine: a neuroactive substance in insects.a compound was isolated from the blood of silkworm larvae, bombyx mori, which had been prostrated with ddt; this compound increased the spontaneous discharge in the isolated abdominal nerve cord of the american cockroach, periplaneta americana. the compound was identified as l-leucine.19724400064
incubation effect in the cockroach (periplaneta americana). 19734744477
[embryonal muscular development in periplaneta americana l. (blattaria) and antheraea pernyi guer. (lepidoptera)]. 19734792593
characterization of chitinases from haemolymph and cell cultures of cockroach (periplaneta americana). 19744810369
development of periplaneta americana (l.) cell cultures and their infection with enteroviruses. 19744368582
comparative toxicity of acetylcholine mustard (methyl-2-acetoxyethyl-2'-chloroethylamine) in the mouse and american cockroach. 19744417829
bioelectrical activity of isolated ventral nerve cords of the cockroach, periplaneta americana l., treated with toxaphene.effects of toxaphene were studied with the ventral nerve cord (vnc) of the cockroach, periplaneta americana (l). intense forms of activity were observed in isolated nerve preparations treated with toxaphene. latent periods between introduction of toxaphene and onset of intense activity decreased as the concentrations were increased.1974444750
gamma-aminobutric acid and glutamate decarboxylas (l-glutamate 1-carboxy-lyase e.c. 4.1.1.15) in the nervous system of the cockroach, periplaneta americana.i.regional distribution and properties of the enzyme.both the central and peripheral nervous system of the cockroach periplaneta americana contain gamma-aminobutyric acid (gaba) and glutamate decarboxylase (gad). in the central ganglia of the cockroach, an average of more that 60 mumoles of gaba are formed from glutamate (glu) per gram wet weight of tissue per hour. this activity level of the gad apoenzyme is considerably higher than that found in the central nervous system of crustaceans, amphibians, avians and mammals but is similar to that repo ...1975235345
morphology and function of malpighian tubules and associated structures in the cockroach, periplaneta americana.this paper describes the different regions of the malpighian tubules and the associated structures (ampulla, midgut, ileum) in the cockroach, periplaneta americana. there are about 150 tubules in each insect. each tubule consists of at least three parts. the short distal region is thinner than the other parts and is highly contractile. the middle region comprises most of the tubule length and is composed of primary and stellate cells. primary cells contain numerous refractile mineral concretions ...19751152069
effects of uv irradiation on nymphs of blattella germanica and periplaneta americana. 19751184821
blattella germanica, periplaneta americana, and monomorium pharaonis: control with insecticidal baits in an animal building and in insectaries. 19751181441
salmonella in the american cockroach: evaluation of vector potential through dosed feeding experiments.restrained american cockroaches, periplaneta americana l., were fed graded doses of salmonella typhimurium ranging from 1-6 x 10(3) to 2-0 x 10(6), and their faeces assayed daily for the pathogen. only 4 specimens out of 117 demonstrated multiplication of salmonellas, which was unrelated to size of input. when data of persistence regardless of actual numbers were expressed as percentage salmonella-positive faecal-days, and these transformed to probits, a graph of percentage-positive faecal-days ...1976789761
fine structure of the rectum in cockroaches (dictyoptera): general organization and intercellular junctions.the organization of the rectal pads is described in cockroaches belonging to the groups blattoidea (periplaneta americana, blatta orientalis) and blaberoidea (supella supellectillu, blaberus craniifer). in the blattoidea, each pad is composed of two layers (principal and basal cells) and is surrounded by very narrow junctional cells supporting the sclerotized cuticle of the pad frame; basally, the junctional cells abut on to the basal cells. in the blaberoidea, the basal cell layer is discontinu ...1976941139
biochemical and physiological effects of chlordimeform.chlordimeform is a relatively new acaricide/insecticide, whose mode of action we have investigated. it appears to interfere with amine-mediated control of nervous and endocrine systems in a variety of ways. specifically, chlordimeform causes a build-up of the amines 5-hydroxytryptamine and to a lesser extent norepinephrine in the rat brain in vivo, antagonizes the in vivo action of reserpine in the rat (reserpine depletes amine stores in the cns), inhibits monoamine oxidase from rat liver in vit ...1976976227
periplaneta americana (l.) as intermediate host of moniliformis moniliformis (bremser) in penang, malaysia. 19761025741
the cystacanth of moniliformis moniliformis (bremser, 1811) and its relationship with the haemocytes of the intermediate host (periplaneta americana).the nature of the capsule of the cystacanth of moniliformis moniliformis was studied. fat body, tracheal system, malpighian tubules and haemocytes of the host are intimately associated with the cystacanth. the capsule of the cystacanth is cellular. the cells encapsulating the middle and late acanthellae are granular haemocytes of the host, whereas those encapsulating the cystacanth are agranular. degranulation of haemocytes after encapsulation of the cystacanth recalls the common method for enca ...1977919698
effects of antibiotics on the survival of salmonella in the american cockroach.the faecal survival of salmonella typhimurium orally administered to restrained american cockroaches maintained on an antibiotic diet was investigated. significant reductions in median numbers of total aerobic faecal microorganisms were noted in insects fed antibiotics daily, and when salmonella was introduced to this modified micro-environment, it persisted for 44 days in all specimens. multiplication of the pathogen was also observed in these insects, with numbers often exceeding 10(3) times t ...1977336789
cockroaches as vectors of sarcocystis muris and of other coccidia in the laboratory.to assess the roles of the german cockroach (blatella germanica) and the american cockroach (periplaneta americana) in the transmission of sarcocystis muris and of 3 other coccidia of cats-toxoplasma gondii, isospora felis, and isospora rivolta, cockroaches exposed to feces containing these coccidia were periodically fed to mice, as was a portion of the fecal matter. sarcocystis muris sporocysts, which in feces remained infectious for at least 20 days, were also transmitted to mice by p. america ...1978417163
immunolatex localization by scanning electron microscopy of intestinal bacteria from cockroaches.an immunolatex reagent was developed from antiserum to fusobacterium varium, an obligate anaerobe isolated from the colon of the cockroach, periplaneta americana l. a new technique that enabled the preparation of a highly efficient immunolatex conjugate was used to localize the bacterium with scanning electron microscopy, in situ, in the mixed gut contents.1978623460
fate of a bacteriophage in aedes aegypti, anopheles quadrimaculatus (diptera: culicidae), and periplaneta americana (orthoptera: blattidae). 1978624847
intestinal microbial flora of the of the american cockroach, periplaneta americana l.a morphological study employing scanning and transmission electron microscopy was made by the alimentary tract of the american cockroach, periplaneta americana l. a. complex microbiota of diverse morphology, which could not be readily dislodged, was observed and found to be restricted to the hindgut, particularly the colon. numerous filamentous forms were noted, and some are described, including the morphologically distinct methanospirillum. flora was noted attached to the cuticular lining and c ...1979543705
phylogenetic distribution of [3h]kainic acid receptor binding sites in neuronal tissue.the phylogenetic distribution of specific binding sites for kainic acid was determined in 14 species including invertebrates and vertebrates. the highest level of binding was observed in brains of the frog (xenopus laevis), followed by the spiny dogfish (heterodontus francisci), the goldfish (carasius auratus) and the chick (gallus domesticus). although significant specific binding was noted in some of the lowest forms tested (e.g. hydra littoralis), this was not a consistent observation in the ...19806247025
insect erythrocyte agglutinins. in vitro opsonization experiments with clitumnus extradentatus and periplaneta americana haemocytes.the effect of naturally occurring haemagglutinins on the in vitro phagocytosis of sheep erythrocytes by the blood cells (haemocytes) of clitumnus extradentatus and periplaneta americana was studied. the results showed that the haemagglutinins in both species failed to act as opsonins. indeed, in some instances, incubation of erythrocytes in haemolymph resulted in less avid ingestion as compared with the saline-incubated controls. this reduced phagocytosis was probably caused by the clumping of e ...19807000682
immunocytochemical identification of neural elements in the central nervous systems of a snail, some insects, a fish, and a mammal with an antiserum to the molluscan cardio-excitatory tetrapeptide fmrf-amide.with an antiserum to the molluscan cardio-excitatory tetrapeptide fmrf-amide neurons and/or nerve fibers were immunocytochemically identified in the central nervous systems of a snail (lymnaea stagnalis), some insects (leptinotarsa decemlineata, periplaneta americana, locusta migratoria, pieris brassicae), a fish (poecilia latipinna) and a mammal (mouse). the fact that immunoreactive material was observed in neurohaemal organs (corpora cardiaca of the insects) as well as in axon terminals ending ...19807006829
morphogenesis of the proboscis hooks of an archiacanthocephalan, moniliformis moniliformis (bremser 1811) travassos 1915.proboscis hooks of moniliformis moniliformis were noted first as small protrusions on developing proboscides of 20- to 28-day-old acanthellae from hemocoels of experimentally infected cockroaches (periplaneta americana). on the basis of proximity, residual connecting strands of tissue, and ultrastructural similarity, origin of the hooks has been determined to be from primordial muscle tissue. during development, primordial proboscis hooks extended into hypodermal tissue, but remained separated b ...19807218121
alcoholic bouin fixation of insect nervous systems for bodian silver staining. ii. modified solutions.a previously devised synthetic equivalent of 'aged alcoholic bouin (duboscq-brasil) fixative was modified in various ways to discover which of the chemical changes brought about by aging were important in improving fixation and staining. effects were tested with ventral nerve cord ganglia of the cockroach periplaneta americana, locust schistocerca gregaria, and honey bee apis mellifera. formation of reaction products, chiefly ethyl acetate and diethoxymethane, seemed to play only a subsidiary ro ...19806161444
humoral mechanisms in the immune response of insects to larvae of hymenolepis diminuta (cestoda).previous work has shown that larvae of hymenolepis diminuta are not encapsulated by the haemocytes of the locust, schistocerca gregaria, but are encapsulated by haemocytes of the cockroach, periplaneta americana. the specificity of the cellular immune response in vivo, and of the agglutinating activity of the sera against vertebrate erythrocytes in vitro, is known to differ between the two species of insect. accordingly, the sera of each of these species was tested for its agglutinating activity ...19817301407
insect chromatophorotropic factors: the isolation of polypeptides from periplaneta americana and apis mellifera with melanophore-dispersing activity in the crustacean, uca pugilator. 19817215775
the capacity of insect flight muscle for the intracellular transport of phospholipids.the postmitochondrial supernatant of the homogenate of metathoracic musculature of periplaneta americana was found to mediate the transfer of labelled phosphatidylcholine and phosphatidylinositol from the rat liver microsomal fraction to unlabelled rat liver mitochondria at 25 degrees c; no transfer occurred at 0 degrees c. the transfer of phosphatidylethanolamine by the insect muscle extract could not be demonstrated conclusively. the transfer activity per mg protein of the supernatant fraction ...19826214807
localization of pancreatic polypeptide (pp)-like immunoreactivity in the central and visceral nervous systems of the cockroach periplaneta.the central and visceral nervous systems of the cockroach periplaneta americana were studied by means of the peroxidase-antiperoxidase immunocytochemical method, with the use of antibody to bovine pancreatic polypeptide (pp). pp-like immunoreactive neuron somata are most numerous in the brain; at least 6 pairs of cell groups occur in clearly defined regions. three pairs of cells each are also present in the suboesophageal ganglion and the thoracic ganglia, one pair of single cell each in the fir ...19826756638
effects of palytoxin on sodium and potassium permeabilities in unmyelinated axons.palytoxin (ptx) is a very potent marine toxin isolated from the zoanthid palythoa. its effects on the nerve membrane have been studied on two different preparations: the giant axon of the cockroach, periplaneta americana and the giant axon of the squids, loligo pealei and loligo forbesi. in both preparations, ptx at concentrations ranging from 0.03 to 10 microm depolarized the axonal membrane through a selective increase in the resting sodium conductance. this increase was never reversible and w ...19826123163
differential accessibility to two insect neurones does not account for differences in sensitivity to alpha-bungarotoxin.the nicotinic acetylcholine receptor probe alpha-bungarotoxin (1.0 x 10(-7) m) blocks the depolarising response to ionophoretic application of acetylcholine onto the cell body membrane of the fast coxal depressor motoneurone (df) of desheathed cockroach (periplaneta americana) metathoracic ganglia, but at the same concentration is completely ineffective in blocking the depolarising action af acetylcholine on dorsal unpaired median (dum) neurones in the same ganglion. the possibility that this is ...19827147226
evidence for similarities between cyclodiene type insecticides and picrotoxinin in their action mechanisms.evidence has been obtained to indicate that cyclodiene-type insecticides, e.g., heptachlor epoxide and gamma-bhc, mimic the action of picrotoxinin. these insecticides inhibit the gaba (gamma-aminobutyric acid)-stimulated chloride uptake in the coxal muscle of the american cockroach, and directly compete against [3h]a-dihydropicrotoxinin for binding in the rat brain synaptosomes. moreover, several cyclodiene-resistant insect strains are also resistant to picrotoxinin. this cross-resistance is spe ...19836187794
biochemical toxicology of lindane and its analogs.the inhibitory potencies of bhc isomers against na+-k+- atpase, yeast growth and nerve conduction of the american cockroach were determined. the insecticidal and neuroexcitatory activities of a number of analogs with the same configuration as lindane in which some chlorine atoms had been replaced by other substituents were also examined against american cockroaches. the structure-activity relationships and the mode of action of lindane and its analogs were discussed. their metabolic fate was stu ...19836187795
immunological recognition of cuticular transplants in insects.transplantation of allogeneic and xenogeneic cuticle onto the american cockroach, periplaneta americana, was carried out in order to compare the specificity of immune recognition of these 'skin grafts' with that of implanted tissues. in order to facilitate interpretation of results, the technique of transplanting cuticle from nymphal donors onto nymphal recipients was adopted - if donor subcuticular epidermis is not recognised as 'foreign', it will grow, fuse with the recipient's epidermal sheet ...19836341106
udpglucosyltransferase and its kinetic fluorimetric assay.a rapid, kinetic assay for udpglucosyltransferase has been developed using 1-naphthol as substrate. it is based on the continuous fluorimetric monitoring of 1-naphthyl glucoside formation during the reaction at physiological ph. the conjugate is easily distinguished from aglycone, since their fluorimetric properties differ. glucoside biosynthesis in vitro by microsomal preparations isolated from the gut and fat body of cockroaches periplaneta americana and leucophaea maderae, and from the green ...19836218988
laboratory studies on the susceptibility of blattella germanica and periplaneta americana to bacillus thuringiensis var. israelensis. 19846523178
isolation and characterization of two myoactive neuropeptides: further evidence of an invertebrate peptide family.the neuropeptide proctolin acts as a neuromuscular co-transmitter in insect skeletal muscle. as a prelude to determining whether other peptides may function in a similar way, we are attempting to isolate and characterize the chemical nature of new myoactive neuropeptides in insects. we examined the corpus cardiacum, a major insect neurosecretory structure of the american cockroach (periplaneta americana), using a skeletal muscle bioassay and high pressure liquid chromatography fractionation and ...19846699686
controlled release effect of insecticide microcapsules and their results in common household insect pest control.since 1978 we have prepared microencapsulated insecticides by complex coacervation and interfacial polymerization for household insect pest control, fenthion being the main core element. a series of tests have been conducted against mosquitoes (culex pipiens quinquefasciatus), cockroaches (periplaneta americana) and bedbugs (cimex lectularius), and the results indicate that microencapsulation can prolong persistent effects by 2-12 times, enhance safety and subdue offensive odour. furthermore, th ...19846336530
metabolism of proctolin, a pentapeptide neurotransmitter in insects.the in vitro metabolism of [tyrosyl-3, 5-3h]proctolin (h-arg-tyr-leu-pro-thr-oh) was studied in the following tissues from the american cockroach, periplaneta americana: proctodeum, midgut, hemolymph, brain, terminal ganglion, and coxal depressor muscles. in all tissues assayed, the tyr-leu bond is the primary cleavage site, but scission of the arg-tyr bond is also significant. greater than 90% of the degradative activity is found in the 100,000 x g supernatant from homogenates. in vivo studies ...19846141517
distribution of 5-hydroxytryptamine and indolealkylamine metabolites in the american cockroach, periplaneta americana l.a procedure is described for simultaneous estimation of tryptophan (tp), 5-hydroxytryptophan (5-ohtp), 5-hydroxytryptamine (5-ht), 5-hydroxyindoleacetic acid (5-hiaa), n-acetyl 5-hydroxytryptamine (na5-ht) and n-acetyldopamine (nada) using high performance liquid chromatography with coulometric electrochemical detection. the procedure has been used to determine the distribution of these compounds in the central nervous system of the american cockroach, periplaneta americana. the ratio of tp:5-ht ...19846083843
localization of crf immunoreactivity in the central nervous system of three vertebrate and one insect species.by use of a specific antiserum against synthetic ovine corticotropin-releasing factor (crf) in the peroxidase-antiperoxidase (pap) immunocytochemical procedure (vandesande and dierickx 1976), crf-like antigenic determinants were demonstrated in the central nervous system of a human fetus, the wistar rat, the frog rana ridibunda, and the american cockroach periplaneta americana. the immunoreactive crf-producing cells occur mainly in the nucleus paraventricularis of the rat, while in rana ridibund ...19846091891
naturally occurring agglutinins against trypanosomatid flagellates in the haemolymph of insects.in vitro studies of the behaviour of the trypanosomatid flagellates trypanosoma brucei and leishmania hertigi in the presence of cell-free haemolymph of locusts, schistocerca gregaria and cockroaches, periplaneta americana revealed the presence of parasite agglutinins. the range of normal values of agglutination titres was 2(-4) to 2(-13). physico-chemical treatment of haemolymph indicated that these agglutinins are protein or glycoprotein in nature and are only partially affected by heat treatm ...19846096791
a comparative study of the size-heterogeneous high mannose oligosaccharides of some insect vitellins.comparative studies of the carbohydrate component from vitellins of the cockroaches blattella germanica, blaberus discoidalis, periplaneta americana and simploce capitata and the locust locusta migratoria have been conducted. chemical, enzymatic and chromatographic analyses show that each vitellin contains variably processed high mannose type oligosaccharides. while all have a common size range they occur as two distinct classes based on the proportion of individual saccharides present. oligosac ...19846509925
the humoral immune response in the american cockroach, periplaneta americana: reactivity to a defined antigen from honeybee venom, phospholipase a2.our previous experiments demonstrated that honeybee venom could induce a specific, adaptive humoral immune response in the american cockroach. since honeybee venom is a complex substance made up of several proteins, a more defined antigen is needed for future characterization studies. one of the components of bee venom, phospholipase a2 (pa2) was found to be highly lethal and immunogenic in the roach. roaches injected with pa2 generated a specific primary response that developed over a period of ...19846519337
[the role of esterases in the toxicity of organothiophosphorus insectoacaricides containing a fragment of mercaptoacetic acid].interaction of insectoacaricide me (eto)p(s)sch2sch2coome (i), its activation metabolites (p = o (ii), s = o, and p = o, s = o (iii) analogues), and a detoxication product (-cooh analoque (iv) with rat liver carboxylesterase, acetylcholinesterase and butyrylcholinesterase of warm-blooded animals, as well as with cholinesterase and carboxylesterase of american cockroach has been studied. low toxicity of (i) towards warm-blooded animals and american cockroach is shown to result from its rapid hydr ...19854052120
insect adipokinetic hormones.peptides with adipokinetic (and usually carbohydrate-mobilizing) potency have been demonstrated in various insects, including locusta migratoria, schistocerca gregaria, manduca sexta, danaus plexippus and periplaneta americana. as far as characterized by now the adipokinetic factors are blocked peptides, consisting of eight to ten amino acid residues. in locusts the adipokinetic hormones are synthesized in the glandular lobe of the corpus cardiacum and released into the haemolymph in response to ...19853831971
dopamine-beta-hydroxylase-like immunoreactive neurons in two insect species, calliphora erythrocephala and periplaneta americana.the localization of dopamine-beta-hydroxylase in the cephalic central nervous system of the blowfly (calliphora erythrocephala) and the cockroach (periplaneta americana) was investigated. immunoreactive neurons were demonstrated in both species. the results were compared with the known distribution of catecholamines in the brain of both species. in certain cell groups and neuropilar regions of both species d beta h-immunoreactivity coincides with the presence of catecholamines. additionally d be ...19853930439
distinct localization of fmrfamide- and bovine pancreatic polypeptide-like material in the brain, retrocerebral complex and suboesophageal ganglion of the cockroach periplaneta americana l.one bovine pancreatic polypeptide (bpp) antiserum and two fmrfamide antisera were applied in the peroxidase-antiperoxidase (pap) immunohistochemical technique on a complete series of sections of brains, suboesophageal ganglia (sog), corpora cardiaca (cc) and corpora allata of periplaneta americana l. double immunohistochemical staining demonstrated that the same perikarya and processes were stained by both the bpp and fmrfamide antisera. this was caused by cross-reaction of the bpp and fmrfamide ...19852416381
binding of n-[propionyl-3h]propionylated alpha-bungarotoxin and l-[benzilic-4,4'-3h] quinuclidinyl benzilate to cns extracts of the cockroach periplaneta americana.the nerve cord of the cockroach (periplaneta americana) contains distinct saturable components of specific binding for the ligands n-[propionyl-3h]propionylated alpha-bungarotoxin and l-[benzilic-4,4'-3h]quinuclidinyl benzilate. n-[propionyl-3h]propionylated alpha-bungarotoxin bound reversibly to homogenates with a kd of 4.8 nm and bmax of 910 fmol mg-1. the association rate constant (1.9 x 10(5) m-1 s-1) and dissociation rate constant (1.2 x 10(-4) s-1) yielded a kd of 0.6 nm. nicotinic ligands ...19852858352
ontogeny of the invertebrate humoral immune response: studies on various developmental stages of the american cockroach (periplaneta americana).earlier studies revealed that a specific adaptive humoral immune response can be induced in the american cockroach (periplaneta americana) to the soluble protein complex, honeybee venom (hbt). we have undertaken a series of ontogenetic studies to determine if there are differences between the protective responses of roaches representing different developmental stages. our results indicated that the response to hbt in the immature cockroach (nymphs weighing 200-500 mg) was characterized by a sign ...19854043479
effect of gender on the inducible humoral immune response to honeybee venom in the american cockroach (periplaneta americana).it is well known that in higher animals, the female of the species usually possesses a superior immune response to that of the male. we investigated the possibility that this rule of nature might also be true amongst the invertebrates. adult female american cockroaches (periplaneta americana) were immunized with honeybee venom, and their responses were compared to that of the adult male. the female primary response was found not only to be enhanced, but prolonged as compared to the male. the res ...19853996707
distribution of calmodulin in insects as determined by radioimmunoassay.the distribution of calmodulin in 11 separate tissues of the cockroach leucophaea maderae was determined by radioimmunoassay. the highest levels of this protein were found in malpighian tubules, visceral muscle and the central nervous system. distribution of calmodulin was also determined for the following insect species: apis mellifera, canthon imitator, heliothis zea (larvae) and periplaneta americana. abdominal tissues contained the highest levels of the calcium binding protein, while lesser ...19852861002
morphology of identified neurosecretory and non-neurosecretory cells in the cockroach pars intercerebralis.electrophysiologically identified cells of the cockroach pars intercerebralis (periplaneta americana) were injected with the dye lucifer yellow for morphological examination and with horseradish peroxidase for ultrastructural marking. in addition to this, uninjected cells were also studied to elaborate the findings from the injected material. the two electrophysiologically distinct classes of cells (type i and type ii) correspond to two distinct morphological and ultrastructural classes. type i ...19852582075
substance p-like immunoreactivity in the central nervous system of the blattarian insect periplaneta americana l. revealed by a monoclonal antibody.brains, retrocerebral complexes and frontal and suboesophageal ganglia of adult american cockroaches, periplaneta americana, were immunohistochemically investigated with a specific monoclonal antibody (mcab) directed against a well characterized antigenic determinant, namely the cooh terminus of the endecapeptide substance p (sp). this resulted in the detection of several neurons and nerve fibres containing a substance antigenically closely related to this typically vertebrate neuropeptide. no d ...19852417998
hemolymph transfer as an assay for immunorecognition in the cockroach periplaneta americana and the locust schistocerca gregaria.a quantitative assay for measuring the number of hemocytic nodules formed in response to foreign particles and soluble molecules has been used, in the locust schistocerca and the cockroach periplaneta, to investigate the response to transferred hemolymph. xenogeneic test particles, rabbit neutrophil leukocytes, stimulate formation of nodules when injected into both insect species, compared with saline-injected controls. however, the number of nodules formed in the locust in response to cockroach ...19863952802
ultrastructural demonstration of nonsynaptic release sites in the central nervous system of the snail lymnaea stagnalis, the insect periplaneta americana, and the rat.release of neuronal secretory products by exocytosis was studied ultrastructurally in the central nervous systems of three different species (the snail lymnaea stagnalis, the cockroach periplaneta americana and the rat). tissues were fixed with: (1) a mixture of glutaraldehyde and osmium tetroxide, (2) the tannic acid-glutaraldehyde-osmium tetroxide (tago) method, and (3) the tannic acid-ringer incubation (tari) method. especially after tari-treatment, release of the contents of the secretory ve ...19863703256
[selectivity of the effect on cholinesterase of stereoisomers of thiophosphonates with asymmetrical phosphorus atoms].the inhibitory action of stereoisomers of organophosphorous compounds with asymmetric phosphorous atom, (ch3)2cho(ch3)p(o)sch2ch2sc2h5 and (ch3)2cho(ch3). .p(o)(ch2)3ch3, on acetylcholinesterase from nervous ganglia of cockroach periplaneta americana and bovine erythrocytes as well as on horse serum butyrylcholinesterase was studied at ph 7.5 and 25 degrees c. it was found that the interaction of the inhibitors with cholinesterases has a complex type and includes reversible and irreversible stag ...19863778536
[study on the time limitation of carrying yersinia enterocolitica by periplaneta americana and flies]. 19863815490
isolation of campylobacter subsp. jejuni from oriental and american cockroaches caught in kitchens and poultry houses in vom, nigeria.a total of 690 adult cockroaches (periplaneta americana (l.), the american cockroach, and blatta orientalis (l.), the oriental cockroach) were captured alive within domestic kitchens and near poultry houses in vom. using selective media, their external surfaces and internal (gut) contents, after adequate decontamination of the external surfaces, were culturally examined for the presence of campylobacters. 4 isolates of campylobacter subsp jejuni were made (0.5%); 3 from the gut contents and 1 fr ...19863557829
immunocytochemical staining of central neurones in periplaneta americana using monoclonal antibodies to choline acetyltransferase.immunocytochemical mapping of cholinergic neurones in the cns of the cockroach periplaneta americana has been attempted using monoclonal antibodies to choline acetyltransferase (chat, acetyl-coa: choline o-acetyltransferase, ec 2.3.1.6). monoclonal antibodies 11 255 and 1e6 raised against rat brain chat and 1c8 raised against drosophila melanogaster chat were ineffective in staining periplaneta neurones. however, the cytoplasm of certain neuronal cell bodies was stained by monoclonal antibody 4d ...198618620156
acetate synthesis from h(2) plus co(2) by termite gut microbes.gut microbiota from reticulitermes flavipes termites catalyzed an h(2)-dependent total synthesis of acetate from co(2). rates of h(2)-co(2) acetogenesis in vitro were 1.11 +/- 0.37 mumol of acetate g (fresh weight) h (equivalent to 4.44 +/- 1.47 nmol termite h) and could account for approximately 1/3 of all the acetate produced during the hindgut fermentation. formate was also produced from h(2) + co(2), as were small amounts of propionate, butyrate, and lactate-succinate. however, h(2)-co(2) fo ...198616347157
apparent differences in tryptophan hydroxylation by cockroach (periplaneta americana) nervous tissue and rat brain.tryptophan hydroxylation in cockroach (periplaneta americana) nervous tissue was measured and compared to the hydroxylation of tryptophan in rat brain. tryptophan hydroxylation in both tissues requires a pterine cofactor, and is inhibited by p-chlorophenylalanine. the molecular weight of the protein responsible for hydroxylation of tryptophan in cockroach nervous tissue obtained from gel filtration was estimated to be 54,000. the ph optima and enzyme kinetics differed greatly between the two hyd ...198720501170
[occurrence, distribution and control of cockroaches in the east german district of rostock].the german cockroach (blattella germanica) is the prevalent species in the district of rostock. there was also an occurrence of blatta orientalis and, in one case, of periplaneta australasiae. periplaneta americana is occasionally introduced from warmer regions of the globe via containers. extent and intensity of infestation of many buildings has increased making large-scale control measures necessary. most control measures have been taken in food industry enterprises. the quantity of cockroach ...19873605784
purification and some properties of a lectin from the hemolymph of periplaneta americana (american cockroach).a lectin showing specificity for human a-type red blood cells was purified to homogeneity from the hemolymph of the american cockroach periplaneta americana by affinity chromatography on bovine submaxillary gland mucin. this lectin was a huge molecule with molecular mass of about 1500 kda, with a single subunit of 30-kda protein, and required ca2+ for expression of lectin activity. electron microscopic examination showed that these molecules were rods with helical structure with an average lengt ...19873665920
defensive secretion of the tenebrionid beetle, blaps mucronata: physical and chemical determinants of effectiveness.the primary components of the defensive secretions of blaps mucronata (tenebrionidae) are two quinones (methyl-p-benzoquinone and ethyl-p-benzoquinone) and the hydrocarbon 1-n-tridecene. the hydrocarbon is shown, by comparison with longer- and shorter-chain n-alkanes and 1-n-alkenes, to be optimally suited as carrier of the quinones, and as a surfactant that promotes spread of secretion over the beetle's body following discharge from the gland openings at the abdominal tip. as shown from repelle ...19873668879
the effect of biotic and abiotic implants on the recognition of blatta orientalis cuticular transplants by the cockroach periplaneta americana.the immune system of the american cockroach, periplaneta americana, was activated to recognise as "non self" xenogeneic tissue normally treated as "self". activation was accomplished by injecting into the insect haemocoele material known to elicit an encapsulation response such as blaberus craniifer cuticular tissue and 6b-sepharose beads. in these insects, cuticular skingrafts from blatta orientalis, a closely related species of cockroach, were rejected by more than half of the recipients. ther ...19873297828
survival of shelled acanthors of moniliformis moniliformis (acanthocephala) under laboratory conditions.shelled acanthors (= eggs) of two isolates of moniliformis moniliformis (acanthocephala) were tested for their capacity to respond to a hatching stimulus in vitro and for their retention of infectivity to a natural intermediate host (periplaneta americana). the shelled acanthors were stored for more than 120 weeks (australian isolate) and for 104 weeks (texan isolate) together with rat faeces in an incubator maintained at 22.2 +/- 0.1 degrees c. in both cases, infectivity to p. americana was sho ...19873437111
a hypoglycemic factor from the corpus cardiacum of the american cockroach periplaneta americana.thin-layer chromatography of cockroach corpora cardiaca (cc) extracts revealed a fraction (f1 in solvent system 1) which had hyperlipemic activity in locusts and which differed in mobility from synthetic locust adipokinetic hormone i (akh i). fractions 4 and 5 exhibited hypolipemic activity when injected into locusts. pooled samples of these fractions caused a diminution of carbohydrate levels in the cockroach. bovine insulin mimicked both these effects. no change in hemolymph lipid was evident ...19873556312
pharmacology of putative glutamate receptors from insect skeletal muscles, insect central nervous system and rat brain.binding of [3h]glutamate to housefly brain and honeybee brain and thoracic muscle membranes as well as to the american cockroach nerve cord was measured in na+-free tris-citrate buffer, 2.5 mm cacl2, ph 7.4. the dissociation constants (kds) ranged from 0.16 to 1.36 microm, and thoracic muscles had 2-4-fold higher density of receptors than brain tissue. the potent inhibitors of housefly brain binding were in decreasing order of effectiveness: l-glutamate greater than l-aspartate = l-cysteate = ib ...19872885148
some aspects on l-dopa decarboxylase and p-tyrosine decarboxylase in the central nervous and peripheral tissues of the american cockroach periplaneta americana.1. aromatic amino acid decarboxylase activities toward l-dopa (l-3,4-dihydroxyphenylalanine), 5-htp (5-hydroxytryptophan) and p-tyrosine in different tissues of the sclerotized and newly ecdysed cockroach were analyzed. 2. the ratios of enzyme activity with regard to l-dopa and p-tyrosine varied considerably in the tissues and between the two different growth stages. 3. a dopa decarboxylase and a p-tyrosine decarboxylase were separated by gel filtration and ion exchange chromatography. 4. the op ...19872888574
distribution of glutamate decarboxylase-like immunoreactivity in the sixth abdominal ganglion of the cockroach periplaneta americana.an antiserum against glutamate decarboxylase (gad) of the rat brain was used to locate gad activity in sections of the nervous system of the cockroach, periplaneta americana. the sixth abdominal ganglion was chosen because electrophysiological evidence suggests the presence of gabaergic inhibitory synapses in the cercal-giant interneuron system. groups of somata and numerous fibres and tracts were positively labelled by the gad antiserum. a posterior group of labelled somata could be identified ...19883046749
organization of a complex movement: fixed and variable components of the cockroach escape behavior.the escape behavior of the cockroach periplaneta americana was studied by means of high speed filming (250 frames/s) and a computer-graphical analysis of the body and leg movements. the results are as follows: 1. the behavior begins with pure rotation of the body about the posteriorly located cerci, followed by rotation plus forward translation, and finally pure translation (figs. 1, 2). 2. a consistent inter-leg coordination is used for the entire duration of the turn (fig. 3a). at the start of ...19883184004
cockroach hypersensitivity: isolation and partial characterization of major allergens.two allergenic fractions in crude american cockroach extract have been isolated with sephadex g-150 sf gel filtration and diethylaminoethylcellulose ion exchange chromatography. the partially purified fractions of cr-pi and cr-pii elicit 73% and 93% of patients sensitive to the crude extracts, respectively. crude extract contains at least 29 antigenic components, as demonstrated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and crossed immunoelectrophoresis. eighteen allergenic ar ...19883192860
sequence analyses of two neuropeptides of the akh/rpch-family from the lubber grasshopper, romalea microptera.two neuropeptides with adipokinetic activity in locusta migratoria and hypertrehalosaemic activity in periplaneta americana were purified by high-performance liquid chromatography from the corpus cardiacum of the lubber grasshopper, romalea microptera. the sequences of both peptides, designated ro i and ro ii, were determined by gas-phase sequencing employing edman degradation after the n-terminal pyroglutamate residue was enzymatically deblocked, as well as by fast atom bombardment mass spectro ...19883226948
photoaffinity labeling of scorpion toxin receptors associated with insect synaptosomal na+ channels.photoreactive and radioiodinated derivatives of several scorpion toxins acting on insect na+ channels were prepared without loss of their pharmacological activities. photoaffinity experiments were carried out on a synaptosomal fraction from the nerve cord of the cockroach periplaneta americana: with all toxin derivatives, a single specifically labeled band was obtained with a molecular weight of 188,000 +/- 12,000 (n = 17). these results indicate for the first time the molecular weight of the sc ...19882450534
the potency of gamma-1,2,3,4,5,6-hexachlorocyclohexane (lindane).the potency of gamma-1,2,3,4,5,6-hexachlorocyclohexane (lindane) as a convulsant was examined in rats by infusion into a tail vein of the unrestrained animal using a lipid emulsion as vehicle and a dose rate of 200 micrograms/min. the concentration of drug in brain rose linearly up to a convulsant level, the rate of rise increasing with relative organ mass. the decline from a convulsant level was biphasic with half times of, respectively, 30 min and 3 days. convulsant concentrations (cc) in brai ...19882460971
antigenic/allergenic characterization of american and german cockroach extracts.cockroach allergens have been implicated as clinically significant sensitizing agents in the induction/exacerbation of "urban asthma." in the present study, approximately 50% of atopic, predominantly inner-city residents had immediate wheal-and-flare cutaneous reactivity to a commercial american cockroach whole body extract. crude whole body extracts were prepared in our laboratory from both american (periplaneta americana) and german (blatella germanica) cockroach species. crossed immunoelectro ...19883346487
the role of galactosyl-binding lectin in the cellular immune response of the cockroach periplaneta americana (dictyoptera).agglutinating activity of the major serum lectin of periplaneta americana against mammalian erythrocytes has been found previously to be inhibited most effectively by galactose and glycoproteins rich in non-reducing terminal galactose, such as porcine stomach mucin (psm) and desialylated fetuin and bovine submaxillary mucin. antibody raised against the purified lectin was found, by immunofluorescence, to bind to the surface of washed fixed haemocytes (the cells responsible for cellular immunity ...19883391649
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