Publications

experiments on the carbon dioxide transport by the blood of the edible snail (helix pomotial) the common crab (cancer pagurus l.) and the common lobster (homarus vulgaris m. e.).		1948	18874549
[biological assay of preparations of helix pomatia l..].		1954	13189202
the comparative ability of beta-glucuronidase preparations (liver, escherichia coli, helix pomatia, and patella vulgata) to hydrolyze certain steroid glucosiduronic acids.		1961	13782588
spheroplasts of the yeast candida utilis.	svihla, g. (argonne national laboratory, argonne, ill.), f. schlenk, and j. l. dainko. spheroplasts of the yeast candida utilis. j. bacteriol. 82:808-814. 1961.-the formation and properties of spheroplasts of the yeast candida utilis, produced by digestion of the cell walls with snail gut juice (helix pomatia) in isotonic medium, were studied by phase, interference, and ultraviolet microscopy, and tracer techniques. cells cultivated in the presence of l-methionine, s-methyl-l-methionine, and l-h ...	1961	13918716
a comparison of hydrolase and lytic activities of the digestive juices of the giant african snail, achatina fulica, and helix pomatia.		1963	13981006
some cytological and pathogenic properties of spheroplasts of candida albicans.	kobayashi, george s. (tulane university, new orleans, la.), lorraine friedman, and judith f. kofroth. some cytological and pathogenic properties of spheroplasts of candida albicans. j. bacteriol. 88:795-801. 1964.-spheroplasts of candida albicans were prepared by use of an enzymatic mixture from the digestive tract of the snail helix pomatia. untreated cells exhibited well-defined cell walls, whereas such structures were absent from spheroplasts. the intravenous inoculation of either spheroplast ...	1964	14208520
[studies on the acetylcholinesterase in the blood of helix pomatia. 3. a combined optical test for determining the enzyme activity].		1964	14236172
beta-galactosidase of helix pomatia.		1964	14236292
urinary excretion of c-20-reduction products of corticosterone.	1. a 200 mg. portion of corticosterone was ingested by a healthy man and the urine collected. part of the urine was treated with the gastric juice of helix pomatia and extracted with ethyl acetate, and the extract fractionated with girard t. paper-chromatographic separation of the non-ketonic fraction in the bush (1952) system b(5) revealed the presence of two unknown polar components. 2. the unknown compounds did not possess a reducing (blue tetrazolium) or a reducible (potassium borohydride) g ...	1965	14342242
[biochemical research on the inactivation of 5-hydroxytryptamine at the level of the heart in the mollusca helix pomatia, buccinum undatum, venus mercenaria and mya arenaria].		1965	4221676
[agglutination of escherichia coli with an agglutinin from helix pomatia].		1966	4862038
protoplast formation and yeast cell-wall structure. the action of the enzymes of the snail, helix pomatia.	1. the digestive juice of the snail helix pomatia was used in the study of the degradation of isolated cell-wall preparations from a strain of saccharomyces carlsbergensis. 2. the crude enzyme system was fractionated by gel filtration and the activities of the more specific fractions thus obtained were examined. 3. results are discussed with respect to (a) the nature of various factors that are essential for protoplast formation and cell-wall dissolution and (b) structures envisaged in yeast cel ...	1966	5964965
studies on glycoproteins. xv. substrate-dependence of o-seryl-n-acetylgalactosaminide glycosidase (helix pomatia) activity and mechanism of the enzymatic reaction.		1967	6077032
the hydrolysis of glycosyl fluorides by glycosidases.	1. alpha-d- and beta-d-glucopyranosyl, alpha-d- and beta-d-galactopyranosyl, alpha-d-mannopyranosyl and alpha-d-xylopyranosyl fluorides were hydrolysed specifically by the respective glycosidases from several sources. 2. use of specific inhibitors with a mixture of glycosidases from helix pomatia intestinal juice showed that each glycosyl fluoride was hydrolysed only by the respective glycosidase. alpha-d-glucopyranosidase and alpha-d-xylopyranosidase activities were shown to be due to different ...	1967	5584010
glucose-1-phosphate-uridyl transferase of helix pomatia.		1967	5587408
[comparative studies with protein gland extracts of helix pomatia and helix hortensis].		1967	5627945
[agglutination of streptococci of the group c by an agglutinin from helix pomatia].		1967	4232426
the structure of a glycopeptide isolated from the yeast cell wall.	1. glycopeptides containing mannose were extracted from isolated yeast cell walls by ethylenediamine and purified by treatment with pronase and fractionation on a sephadex column. 2. a glycopeptide that appeared homogeneous on electrophoresis and ultracentrifugation had a molecular weight of 76000, and contained a high-molecular-weight mannan and approx. 4% of amino acids. 3. the amino acid composition of the peptide was determined. it was rich in serine and threonine and also contained glucosam ...	1968	5685868
purification and characterization of a blood-group a reactive hemagglutinin from the snail helix pomatia and a study of its combining site.		1969	4241272
studies on specificity and binding properties of the blood group a reactive hemagglutinin from helix pomatia.		1971	4931750
modification of the composition and structure of the yeast cell wall by culture in the presence of sulfur amino acids.	growth of candida utilis and saccharomyces cerevisiae in a medium supplemented with sulfur amino acids led to synthesis and accumulation of s-adenosylmethionine, accompanied by a reduction in the cell yield, an increased sensitivity of the cell wall to snail gut enzymes (helix pomatia), as judged by spheroplast formation, and by a modification of the chemical composition of both the intact cells and their isolated walls. walls of supplemented cultures of c. utilis were three times as sensitive t ...	1971	4934069
purification of snail hemagglutinin from helix pomatia and its serological characteristics.		1971	5002618
the composition of the cell wall of fusicoccum amygdali.	1. the cell wall of fusicoccum amygdali consisted of polysaccharides (85%), protein (4-6%), lipid (5%) and phosphorus (0.1%). 2. the main carbohydrate constituent was d-glucose; smaller amounts of d-glucosamine, d-galactose, d-mannose, l-rhamnose, xylose and arabinose were also identified, and 16 common amino acids were detected. 3. chitin, which accounted for most of the cell-wall glucosamine, was isolated in an undegraded form by an enzymic method. chitosan was not detected, but traces of gluc ...	1971	5144751
bilirubin glucuronyltransferase. specific assay and kinetic studies.	1. bilirubin glucuronide was synthesized in vitro in a system containing a rat liver microsomal fraction, udp-glucuronic acid, mg(2+) and bilirubin. the enzymic synthesis was accomplished without the addition of a bilirubin carrier. 2. azobilirubin and azobilirubin glucuronide were separated by t.l.c. and paper chromatography and the measurement of the conjugate provided a specific assay for bilirubin udp-glucuronyltransferase (ec 2.4.1.17). 3. this diazo compound was labelled when [u-(14)c]udp- ...	1971	5158913
a new polyvalent proteinase-inhibitor occurring in the albumin gland of helix pomatia.		1971	5173510
[investigations about an alleged lysozym-like behavior of the protectine anti-ahp from the albumen gland of the edible snail (helix pomatia) (author's transl)].		1972	4283254
the reaction of products of sequential periodate oxidation of human erythrocyte glycoproteins with hemagglutinin from helix pomatia.		1972	4351954
precipitation of lipopolysaccharides from rough mutants of salmonella typhimurium by an a-hemagglutinin from helix pomatia.		1972	4557086
[occurrence and partial purification of an unspecific cholinesterase in the albumin gland extract of helix pomatia].		1972	4652484
[new polyvalent protease inhibitors for trypsin, chymotrypsin, plasmin and kallikreins from snails (helix pomatia)].		1972	4260409
polymorphism of esterases and tetrazolium oxidases in the roman snail, helix pomatia: a study of populations from sweden and germany.		1973	4726086
binding of helix pomatia a hemagglutinin to human erythrocytes and their cells. influence of multivalent interaction on affinity.		1973	4728059
the human immune response to alpha-haemocyanin of helix pomatia.		1973	4749168
immunological characterization of blood group a reactive protectins from helix pomatia.		1973	4760392
[various comparative elements of the desamidinase systems in porcine renal tissue and in the hepatopancreatic tissue of helix pomatia lin].		1973	4803138
a new surface marker on t lymphocytes of human peripheral blood.	neuraminidase treatment of human peripheral blood lymphocytes uncovers cell surface receptors that bind purified a hemagglutinin from the snail helix pomatia. no hemagglutinin was bound to untreated lymphocytes. binding studies with (125)i-labeled hemagglutinin suggested that the number of receptors on neuraminidase-treated lymphocytes was approximately 1.10(6)/cell. the apparent association constant for hemagglutinin binding to lymphocytes, as calculated from scatchard's plots, was 5-7.10(8) li ...	1973	4583071
[utilisation of the helix pomatia juice for fractionation of the cell wall of a wild strain and a mutant strain of saccharomyces cerevisiae hansen].		1973	4585301
routine identification of group-c streptococci by means of an agglutinin (protectin) from the albumen gland of the edible snail, helix pomatia.		1973	4632252
comparative studies of anti-a agglutinins from various snails of the genus helix (helix pomatia and helix aspersa).		1973	4196846
cross-reactive human blood group h-active polysaccharide from helix pomatia. i. detection with catfish anti-h and eel sera.		1973	4201825
[molecular weight determination on the protectine anti-hp from the albumin gland of helix pomatia (author's transl)].		1974	4283003
[reaction of anti-a-hemagglutinin from helix pomatia with algae (chlorella pyrenoidosa) and demonstration of heterophilic agglutinins against algae in human sera].		1974	4138180
properties of an antigenic polysaccharide from corynebacterium parvum.	corynebacterium parvum strain 10390 is an antitumor agent and stimulant of the reticuloendothelial system and produces a soluble antigen towards the end of its growth cycle. this material, which is a cell wall component and can also be released from the organism by acid or alkaline hydrolysis, has been purified. it is an acidic polysaccharide of molecular weight 100,000 to 150,000 and contains galactose, glucose, fucose, n-acetylgalactosamine, n-acetylglucosamine, uronic acids, sialic acids, and ...	1974	4138438
[stimulation of experimentally inducdd tissue granulation by agglutinin obtained from helix pomatia l. albumin glands (anti-aph)--part ii (author's transl)].		1974	4141666
studies on the hatching of miracidia of dicrocoelium dendriticum (author's transl).	the eggs of dicrocoelium dendriticum were induced to open by solutions of formic acid and caproic acid (table 1). the miracidia hatched in o2-free water after the eggs had been dried with n2 or in vacuum. the miracidia were able to live for 3 hours if water contained 20 mm nacl, 10 mm kcl, and 1 mm cacl2. ca++-ions are obviously necessary for the mobility of miracidia. the experimental use of intestinal juice of the roman snail helix pomatia gave hatching results which were dependent on the abse ...	1975	3901
universality of gonadotropic hormone effect. effect of mammalian fsh on the ovotestis of the snail helix pomatia.	gonadotropic hormone (fsh) stimulated markedly spermatogenesis in the ovotestis of snails. this is indicative of the presence of receptors sensitive to gonadotropic hormone in snail spermatogonia. fsh had no influence on oogenesis.	1975	168707
quantitative precipitin studies on the specificity of an extract from tridacna maxima (röding).	haemolymph from the clam tridacna maxima precipitated with purified h-blood-group substances, helix pomatia galactogen, and pneumococcus type xiv polysaccharide. although gel diffusion, gel electrophoresis, and inhibition experiments indicated that only a single precipitating lectin was present in the haemolymph, quantitative precipitin and haemagglutination results suggested that a second agglutinin with anti-h-like specificity was also present. evidence obtained from hapten inhibition experime ...	1975	236090
[gelelectrofocusing of protektin anti ahp from the albumin gland of helix pomatia (author's transl)].	the protektin anti ahp from the albumine gland of helix pomatia is a very interesting substance because of its ability to agglutinate erythrocytes of blood group a as well as group c streptococci. purification is possible by reversible absorption on sephadex g200. isoelectric focusing in a sucrose density gradient is difficult, because the protein precipitates at the isoelectric point. the agglutinin, which is homogeneous in disk electrophoresis experiments (in an alkaline gel system as well as ...	1975	242131
concanavalin a and other lectins in the study of tumor cell surface organization.	cell surface structures of two mouse ascites tumors were studied using lectins. the tumors are sublines of the spontaneous mammary adenocarcinoma ta3 in strain a, differing in two main characteristics. subline ta3-st grows only in syngeneic mice and has high expression of h-2 antigens. subline ta3-ha, in contrast, proliferates in all mouse strains, and has low amounts of exposed h-2 antigens. concanavalin a (con a), phytohemagglutinin (pha) and helix pomatia anti a hemagglutinin (hp) were used i ...	1975	1098413
[immunoelectronmicroscopic localization of cell wall antigens in streptococci. ii. localization of the group-specific polysaccharide of group c streptococci with ferritin- and peroxidase-labelled helix pomatia-agglutinin (author's transl)].	the carbohydrate of the cell wall of group c streptococci is one of the best known group-specific streptococcal antigens with respect to its chemical structure. in the present paper, the ultrastructural location of the carbohydrate was investigated by means of immunoelectronmicroscopic techniques. besides group-specific antibodies, the specific binding of an agglutinin (protectin anti-ahp) of the edible snail (helix pomatia) to structures with terminal n-acetylgalactosamine was used to demonstra ...	1975	1154918
[production of candida lipolytica protoplasts].	optimal conditions were found for the production and isolation of the protoplasts of candida lipolytica. the maximum amount of the protoplasts was produced after 90 minutes of the incubation with a crude preparation of the enzyme from helix pomatia (100 mg/g wet biomass). longer incubation results in lysis of the protoplasts and structural damages of the intracellular components. the yield of the protoplasts does not depend on the nature of stabilizing agent. a decrease in the stabilizer concent ...	1975	1160627
[spheroplasts of torulopsis candida yeasts and their oxidizing activity].	the paper describes the conditions in which the spheroplasts of the yeast torulopsis candida ibfm-y-127 with a high respiration rate can be isolated. the preliminary incubation of the cells with sh-reagents has to be carried out in a buffer without an osmotic stabilizer, and the incubation in a medium containing 0.6 m kcl, 0.1 m mgso4, 0.1 mkh2po4, ph 5.2. in these conditions the cells are incubated with the enzyme from helix pomatia during 15 to 20 minutes, and the yield of the spheroplasts is ...	1975	1172174
further characterization of some heterophile agglutinins reacting with alkali-labile carbohydrate chains of human erythrocyte glycoproteins.	the nature of the receptor sites for several agglutinins is characterized by hemagglutination inhibition assays. the inhibitory activity of human erythrocytes glycoproteins, from which sialic acid, sialic acid and galactose or alkali-labile oligosaccharides have been removed, is compared to the inhibitory effect of compounds with known structure. it is shown that the lectin from arachis hypogea and anti-t bind to alkali-labile galactosyl-residues. agglutinins from bauhinia purpurea and variegata ...	1975	1172890
the amino-acid sequence of isoinhibitor k form snails (helix pomatia). a sequence determination by automated edman degradation and mass-spectral identification of the phenylthiohydantoins.	performic-acid-oxidized isoinhibitor k of snails (helix pomatia) was subjected to arginine-directed tryptic proteolysis. six peptide fragments including one overlap peptide from limited cleavage of the arg-3-pro-4 bond were purified to homogeneity. four arginine peptides and the c-terminal peptide were sequenced by automatic edman degradation using a special peptide program. the phenylthiohydantoins were all identified by chemical ionization mass spectrometry, except four cysteic acid residues t ...	1975	1183446
[the enzymatic hydrolysis of cell wall applied to yeasts classification (author's transl)].	the rigid structure of yeast cell wall may be a taxonomic criterion. using the helix pomatia juice (with mercaptoethylamine hydrochloride) as a polyenzymatic system, we have always obtained protoplasts from ascomycetous yeasts and never from basidiomycetous yeasts tested. then, we have applied this enzymatic analysis to the fungi imperfecti yeasts and, according to this test, separated genus looking like basidiomycetous behaviour: trichosporon, pityrosporum, rhodotorula, cryptococcus and non fer ...	1975	1190637
the lack of mitogenic response of neuraminidase-treated and untreated human blood lymphocytes to divalent, hexavalent, or insoluble helix pomatia a hemagglutinin.		1975	1193182
[decomposition of steroids during incubation with beta-glucuronidase and during storage of urine].	androsterone, etiocholanolone, pregnanetriol, dehydroepiandrosterone, pregnanediol, tetrahydrocortisol, 5-pregnenolone and 11-beta-oh-androsterone were incubated with beta-glucuronidase preparations (helix pomatia, bovine liver and e. coli) for 96 hrs at 37 degrees c. after extraction and silylation they were gas-chromatographed. the first 3 steroids were left practically intact. the least decomposition of the last 5 steroids occurred with the liver enzyme. testosterone and 11-ketoandrosterone w ...	1975	765125
[use of an agglutinin from the albumin gland of the edible snail for immunoelectron microscopic localization of cell membrane antigens with terminal n-acetyl-d-galactosamine].		1975	829939
trypsin-kallikrein isoinhibitor k (type kunitz) from snails (helix pomatia). purification and characterization.	a basic proteinase inhibitor, isoinhibitor k, was purified by se-sephadex c-25 column chromatography from the mixture of acid-stable and heat-stable isoinhibitors of the snail (helix pomatia). isoinhibitor k is homogeneous in polyacrylamide gel, cellulose acetate and polyacrylamide-dodecylsulfate electrophoresis. from the electrophoretic mobility in dodecylsulfate-polyacrylamide gel and apparent molecular weight of 6500 +/- 200 was estimated. from the amino acid composition the inhibitor consist ...	1975	1081050
receptors for helix pomatia a haemagglutinin on leukaemic lymphocytes from patients with chronic lymphocytic leukaemia (cll).	blood lymphocytes from thirteen patients with cll were studied for surface-bound ig (sig), fc receptors (ea rosettes), receptors for sheep erythrocytes (e rosettes) and receptors for helix pomatia a haemagglutinin (hp), a carbohydrate-binding protein with specificity for n-acetyl-d-galactosamine and related sugars. fluorescein-labelled hp binds to subpopulations of human peripheral blood lymphocytes (pbl) treated with neuraminidase. in normal peripheral blood, hp binds to the t-lymphocytes while ...	1976	1086751
fractionation of human blood lymphocytes on helix pomatia a haemagglutinin coupled to sepharose beads.	treatment of human blood lymphocytes with neuraminidase has previously been shown to uncover receptors for the a haemagglutinin of the snail helix pomatia (hp). neuraminidase-treated lymphocytes were now fractionated on columns charged with large sepharose particles to which hp had been coupled covalently. hp-receptor negative (hp-) lymphocytes passed the columns while hp-receptor positive (hp+) lymphocytes were retained. the latter cells were eluted by addition of the competitive hapten n-acety ...	1976	1088709
alkali-labile oligosaccharides from glycoproteins of different erythrocyte and milk fat globule membranes.	phenol extraction of horse, sheep, cow, pig and human erythrocyte membranes and human milk fat globule membranes gave glycoprotein fractions, all of which were shown by gas chromatography to contain the reduced disaccharide beta-d-galactosyl (1-3)-n-acetyl-d-galactosaminital after treatment with alkaline borohydride. cow and pig erythrocyte membrane glycoproteins were found however to contain much lower amounts than the erythrocyte membrane glycoproteins of the other species tested. after gel fi ...	1976	963062
determination of arginine in the reactive site of proteinase inhibitors by selective and reversible derivatization of the arginine side chain.	reversible and selective modification of the guanidino group by 1,2-cyclohexanedione or 2,3-butanedione in borate buffer was applied to identify arginine residues in the reactive sites of proteinase inhibitors. the ability of the method to distinguish between arginine and lysine residues was examined. trypsin inhibitors with arginine at the reactive site were almost completely inactivated within 2 h, e.g. the low-molecular weight inhibitors from soybeans (kunitz), peanuts and porcine seminal pla ...	1976	964925
analysis by a plaque assay of igg- or igm- dependent cytolytic lymphocytes in human blood.	when monolayers of bovine erythrocytes (eb) were exposed to purified human blood lymphocytes and either igg or igm fractions of rabbit anti-eb serum, clear zones (plaques) appeared when eb had been lysed by antibody-dependent effector cells (k cells). igg-dependent plaque formation was complete by 20 h of incubation, while the igm-dependent reaction required 40 h. the estimated minimal numbers of plaque forming cells (pfc) were 5.6% (igg) and 2.0% (igm) of the added lymphocytes. inhibition exper ...	1976	993729
[direct action of angiotensin ii on the central neurons].	reactions of the nervous cells in the somatosensory and visual regions of the brain cortex and the frontal hypothalamus in rabbits, as well as of the isolated nervous peripharyngeal ring of the helix pomatia to the microionophoretic application of angiotensin ii (a-ii) was studied. reactions of the neurons in the rabbit brain to a-ii displayed an increase in the spike frequency depending on the quantity of the agent applied. reactions of the frontal hypothalamus neurons showed a lower threshold ...	1976	1026279
the interaction of nonmitogenic and mitogenic lectins with t lymphocytes: association of cellular receptor sites.	the relationship between the surface receptors on neuraminidase-treated human blood lymphocytes for the mitogenic lectins phaseolus vulgaris leukoagglutinin (la), concanavalin a (con a) and soy bean agglutinin (sba) and the non-mitogenic lectin helix pomatia a hemagglutinin (hp) was investigated. two different techniques, co-capping with different fluorochrome-labeled lectins and cell binding-inhibition experiments with 125i-labeled lectins, were used. the results demonstrated that the nonmitoge ...	1976	772792
an ultrastructural analysis of protoplast-spheroplast induction in cryptococcus neoformans.	protoplasts-spheroplasts of a human isolate of cryptococcus neoformans were prepared using the gut enzyme of helix pomatia. the induction process, as studied by transmission electron microscopy, occurred in two stages. early in the induction process, protoplasts-spheroplasts emerged from whole cells through a break in the cell wall-capsule envelope. later, a gradual dissolution of the entire cell wall occurred releasing the intact protoplast-spheroplast. a comparison of protoplasts-spheroplasts ...	1976	788868
occurrence of a blood group a-like substance in eggs of the prosobranch snail pomacea canaliculata.	in the eggs of the prosobranch snails pomacea canaliculata and pomacea insularum a blood group a-like substance has been detected by anti-a from the snails helix pomatia, helix aspersa and cepaea nemoralis.	1976	815102
the disulfide bridges of the trypsin-kallikrein inhibitor k from snails (helix pomatia). thermal inactivation and proteolysis by thermolysin (author's transl).	isoinhibitor k is the main component of the complex mixture of isoinhibitors of broad specificity secreted into the mucus by the roman snail (helix pomatia). the disulfide pairing was determined after the amino acid sequence had been elucidated. two cystine-containing peptides with the disulfide bridges cys32-cys53 and cys32-cys53 plus cys7-cys57 were obtained after thermolytic hydrolysis of the native inhibitor at 80 degrees c and chromatographic separation of the peptides using se-sephadex. th ...	1976	3462
[electron microscopic detection of blood group antigen a on human erythrocytes by means of ferritin- and gold-labelled protectin of helix pomatia].	by means of ferritin- and gold-labelled protectin from the albumen gland of the edible snail helix pomatia the blood group antigen a was located on human erythrocytes of groups a1, a2 and a1b. with erythrocytes of groups o and b the reaction is negative. the antigen is focally distributed on the outer surface of the cell membrane. cells from groups a1 and a1b have an antigen a concentration about 4 times greater than a2 cells. the numbers of particles after tagging with ferritin or gold are comp ...	1976	134531
[neuraminidase induced hemolytic anemia. experimental and clinical observations (author's transl)].	recently, increasing attention has been focussed on the in vivo action of neuraminidase as possible pathogenetical factor of hemolytic anemia and even hemolytic-uremic syndrome. neuraminidase action in red cell membranes results in the release of neuraminic acid, and thereby the uncovering of previously hidden receptors, socalled cryptantigens. with special reference to the phythemagglutinin anti-tah from the peanut (arachis hypogae) and the agglutinin anti-ahp from the albumin gland of the smal ...	1976	1246237
immunochemical detection of the thomsen-friedenreich antigen (t-antigen) on the pig lymphocyte plasma membrane.	a method for the isolation of lymphocytes from pig peripheral blood and preparation of plasma membranes was developed. the method resulted in a ten-fold increase in 5'-nucleotidase activity and neuraminic acid, relative to protein. a cholesterol/phospholipid ratio of 0.93 was obtained. alkaline borohydride treatment of isolated plasma membrane, after desialylation, released the disaccharide beta-d-galactosyl-(1 leads to 3)-n-acetyl-d-galactosaminitol (the immunodominant group of the t-antigen), ...	1976	1278165
tyrosine hydroxylase in snail (helix pomatia) nervous tissue.		1976	5096
improved hydrolysis of urinary 17-hydroxycorticosteroid glucuronides with beta-glucuronidase from helix pomatia, on adding sodium sulfate.	sodium sulfate increases the hydrolysis of urinary 17-hydroxycorticosteroid glucuronides with beta-glucuronidase preparations derived from helix pomatia because it removes the inhibitory activity of urinary high-molecular-weight substances. for maximum hydrolysis of urinary 17-hydroxycorticosteroid glucuronides, the hydrolysis 5 ml of urine, 0.5 ml of 2 mol/liter acetate buffer (ph 5.0) should be conducted in the presence of sodium sulfate (final concentration: 80 g/liter) with (a) 600 fishman u ...	1977	21763
[immunologic properties of various cell fractions of a wild strain and a mutant strain of saccharomyces cerevisiae hansen].	some fractions of the saccharomyces cerevisiae cell wall have been prepared by the action of helix pomatia juice on intact cells. immunosera were obtained by injecting rabbits with these fractions. immunofluorescence reactions, obtained with these sera, show that some fractions of mannopeptides when extracted from a "smooth-colony" mutant strain, have lost antigenic determinants.	1977	70263
hydrolysis of steroid glucuronides with beta-glucuronidase preparations from bovine liver, helix pomatia, and e. coli.	we determined the enzymic activity of beta-glucuronidase preparations from bovine liver, helix pomatia, and escherischia coli with steroid glucuronides and nonsteroid glucuronides as substrates. we also studied the effect of na2so4 on the enzymic hydrolysis of several substrates with the three preparations of beta-glucuronidase. na2so4 increases the rate of hydrolysis of all substrates with beta-glucuronidase from bovine liver. hydrolysis of a steroid glucoronide with beta-glucuronidase from hel ...	1977	189953
a new rat lymphocyte surface marker: characterization and separation of cells with receptors for helix pomatia hemagglutinin.	thirty-two percent of neuraminidase-treated da rat spleen lymphocytes and 48% of lymph node lymphocytes possess receptors for helix pomatia hemagglutinin (hp). moreover, these hp-receptor-bearing cells can be separated from b cells by affinity chromatography on hp-sepharose columns. the virtual absence of immunoglobulin (ig) receptors and the close correlation with reported t-cell content of these lymphoid tissues suggest that hp-receptor lymphocytes are probably t cells and that hp may provide ...	1977	322264
purification of some glycoside hydrolases by affinity chromatography.	two glycoproteins have been isolated from the cell walls of baker's yeast. one is a glucan-protein complex which has been partially characterised as having a branched carbohydrate structure composed of chains of (1 leads to 3)-linked beta-d-glucosyl residues, some of which are attached by (1 leads to 6)-linkages to the main chain. immobilization of this glycoprotein was achieved by covalent attachment to sepharose, and the product was used to isolate a number of (1 leads to 3)-beta-d-glucan hydr ...	1977	409497
studies on the cell-free biosynthesis of beta-lactam antibiotics.	cell walls of cephalosporium acremonium mycelia were lysed by enzyme preparations from either helix pomatia (snail) digestive juice or cytophaga. the yield of protoplasts depended on the lytic-enzyme preparation and the age of the culture, and it increased after the mycelia were pretreated with dithiothreitol. a cell-free preparation, obtained by osmotic lysis of protoplasts, synthesized labelled penicillin n from l-[14c]valine. approx. 0.03-0.06% of the amino acid was incorporated into penicill ...	1977	559491
carbohydrate binding specificity of four n-acetyl-d-galactosamine- "specific" lectins: helix pomatia a hemagglutinin, soy bean agglutinin, lima bean lectin, and dolichos biflorus lectin.		1977	560855
electron microscopic detection of the cryptantigen ahumhp (friedenreich antigen) on human erythrocytes by means of gold-labelled agglutinin from helix pomatia.	using the gold-labelled agglutinin anti-ahp from helix pomatia (edible snail) the ultrastructural site and distribution of the cryptantigen (friedenreich antigen) ahumhp was revealed on neuraminidase treated human o and b erythrocytes. on a1 and a2 cells the antigen was demonstrated simultaneously with the blood group antigen a by labelling the cells with anti-ahp-ferritin followed by removing the neuraminic acid and labelling with anti-ahp-gold. the cryptantigen is focally distributed on the ce ...	1977	602347
radioimmunological determination of urinary tetrahydroaldosterone.	a method is presented for radioimmunological determination of 3alpha, 5beta-tetrahydroaldosterone. it is based upon the reactivity of this steroid with an antiserum induced by the 3-carboxymethyloxime of 18, 21-aldosterone diacetate conjugated with bovine serum albumin. one hundred microliters of urine enzymatically hydrolyzed with an helix pomatia preparation, containing tritiated tetrahydroaldosterone for the yield calculation, were extracted with dichloromethane and chromatographed on a small ...	1977	910248
high-performance liquid chromatographic separation and photometric detection of phospholipids.	a rapid and efficient method for the separation of (phospho)lipids by high-performance liquid chromatography using n-hexane-2-propanol-water mixtures as the solvent system is described. the lipid separation occurs on silica gel columns and the individual components are monitored directly by uv absorption at 206 nm. of a total lipid extract from erythrocytes as well as suboesophageal ganglia of the snail helix pomatia, a complete separation is achieved of cholesterol, phosphatidic acid, phosphati ...	1977	914946
peroxidase and gold complexes of lectins for double labeling of surface-binding sites by electron microscopy.	double labeling experiments were performed for visualization of the binding sites of concanavalin a and anti-ahel (the lectin from helix pomatia). the anti-ahel was labeled with the colloidal gold whereas the membrane bound concanavalin a was demonstrated by an affinity technique using horseradish peroxidase. the two markers used could be clearly distinguished electron microscopically. the specificity of the cell surface double labeling was demonstrated in the control experiments. a topological ...	1977	915241
[effect of 6n2'o-dibutyryl adenosine-3',5'-cyclomonophosphate on the protein synthesis of the subpharyngeal ganglia complex in the mollusk, helix pomatia].	proteins of the helix pomatia abdominal complex were separated by electrophoresis. the electrophoretic mobility of most of the proteins was rf = 0.2--0.6. the abdominal ganglion was incubated in the medium with 1 = (4.5h3)-leucine and 2.10(-6) m 6n2'o-dibutyryladenosine-3',5'-cyclomonophosphate for 3, 6 and 24 hours. it was shown that radioactivity of specific low-mol wt neuronal proteins was increased by the former, but the latter inhibited this process.	1978	218655
studies on lectins. xl. o-glycosyl derivatives of spheron in affinity chromatography of lectins.	free monosaccharides can be used for direct glycosylation of spheron, a spherical macroporous hydroxyalkyl methacrylate-ethylene dimethacrylate copolymer, in a reaction that proceeds at room temperature in dioxane medium under catalysis of dry hcl or bf3. derivatives of l-fucose, d-galactose, d-glucose, d-mannose, n-acetyl-d-galactosamine and n-acetyl-d-glucosamine thus prepared from spheron beads have been shown to be efficient affinity carriers in isolation of lectins from seeds of canavalia e ...	1978	638203
immunochemical detection of the thomsen-friedenreich antigen (t-antigen) on platelet plasma membranes.	platelet plasma membranes were found to possess the disaccharide beta-d-galactosyl (1-3)-n-acetyl-d-galactosamine which was measured by gas chromatography after release by alkaline borohyride treatment and desialylation. immunological evidence using the specific lectins from arachis hypogoea and agaricus bisporus and an anti-t serum confirmed the presence of this disaccharide, the immunodominant group of the thomsen-friedenreich antigen (t-antigen). this receptor was only found after prior neura ...	1978	580492
opsonizing effect of serum and albumin gland extracts on the elimination of human erythrocytes from the circulation of helix pomatia.		1978	632614
substrates for the assay of alpha-l-iduronidase.	procedures are described for the preparation of two disaccharides, 4-o-alpha-l-iduronosyl-2,5-anhydro[3h]mannitol and 3-o-alpha-l-iduronosyl-2,5-anhydro[3h]-talitol, from heparin and dermatan sulfate, respectively. these disaccharides lend themselves to an easy assay of alpha-l-iduronidase which is based on the fractionation of the liberated neutral anhydro[3h]mannitol or anhydro[3h]talitol from the unreacted substrate by adsorption of the latter to dowex 1. investigation of the reaction conditi ...	1978	709885
a 1h nuclear-magnetic-resonance study of the solution conformation of the isoinhibitor k from helix pomatia.	the isoinhibitor k from helix pomatia was investigated by high-resolution 1h nuclear magnetic resonance at 360 mhz. detailed studies of the labile protons and the resonances of the aromatic residues indicated extensive homologies between the spatial structures of the snail inhibitor, the basic pancreatic trypsin inhibitor (kunitz) from bovine tissue and the cos colostrum trypsin inhibitor. comparison of these three homologous inhibitors indicated that the overall flexibility of the globular prot ...	1978	710398
a sulphate metabolite of trimethoprim in goats and pigs.	1. urine from goats and pigs after intravenous administration of [14c]trimethoprim contained an unidentified fraction of 'other water-soluble metabolites'. this fraction accounts for 56% of the administered dose in goat urine and 65% in pig urine. 2. a sulphate metabolite comprising the major part of the latter fraction in both goat and pig urine was isolated by gel filtration and paper chromatography and identified by spectroscopic methods as 2,4-diamino-5-(3,4-dimethoxy-5-sulphooxybenzyl)pyrim ...	1978	726512
interaction of peanut agglutinin with human lymphocytes. binding properties and topology of the receptor site.	the relationship between the mitogenic lectin pna and other mitogenic and non-mitogenic lectins was investigated. pna labelled with 125i was found to bind equally well to t and b lymphocytes, after neuraminidase treatment, with 3.88 times 10(6) and 3.73 times 10(6) binding sites respectively. only the t cell fraction was stimulated, however, and only after neuraminidase treatment. preincubation of neuraminidase-treated cells with non-mitogenic lectins and antiserum which appeared to bind to the ...	1978	726549
enrichment of helix pomatia (hp) lectin binding variant from the ta3st mouse ascites tumor by repeated column selection.		1978	738331
studies on lectins. xxxvi. properties of some lectins prepared by affinity chromatography on o-glycosyl polyacrylamide gels.	a number of lectins has been purified by affinity chromatography on o-glycosyl polyacrylamide gels. the lectins isolated (and the particular sugar ligands used in the affinity carriers) are as follows: anguilla anguilla, serum (alpha-l-fucosyl-), vicia cracca, seeds; phaseolus lunatus, seeds; glycine soja, seeds; dolichos biflorus, seeds; maclura pomifera, seeds; sarothamnus scoparius, seeds; helix pomatia, ablumin glands; clitocybe nebularis, fruiting bodies (all n-acetyl-alpha-d-galactosaminyl ...	1978	563738
receptors for helix pomatia a haemagglutinin (hp) on a subpopulation of human b cells.	receptors for helix pomatia a haemagglutinin (hp) have earlier been found on neuramidinase treated t-lymphocytes in human peripheral blood. in contrast, the majority of the b-lymphocytes, characterized by surface bound igm and/or igd(sig)lack these receptors. double marker experiments with fluorochrome labelled reagents have now shown that a minor fraction (3--24%) of the igm/d bearing lymphocytes in normal human blood also have hp-receptors. these hp+ b-cells constitute approximately 1% of the ...	1978	349677
enzyme immunoassay for total oestrogens in pregnancy plasma or serum.	we have developed an enzyme immunoassay for total oestrogens in pregnancy serum of plasma, using horseradish peroxidase (hrp) as the marker enzyme. a test combination consisting of an antiserum against oestriol-16/17-monosuccinyl-albumin and oestriol-16/17-monosuccinyl-hrp yielded a sensitive system, which reacted to approximately the same extent with oestrone, oestradiol, oestriol and their 16- and 17-conjugates. samples had to be diluted 1 to 10 to avoid interference of plasma factors with the ...	1978	361293
helix pomatia a hemagglutinin: selectivity of binding to lymphocyte surface glycoproteins on t cells and certain b cells.	human and mouse lymphocytes were surface-labeled by lactoperoxidase-catalyzed iodination, or by galactose oxidase oxidation followed by reduction with tritiated sodium borohydride. the labeled cells were lysed with nonidet p-40. proteins binding to helix pomatia a hemagglutinin (hp) were isolated by affinity chromatography on hp-sepharose and analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis and autoradiography. a major cell surface glycoprotein (apparent mol. wt. 150 000, us ...	1978	309819
a new surface marker on mouse natural killer cells: receptors for helix pomatia a hemagglutinin.	natural killer (nk) cells in normal mouse spleen have so far been found to lack conventional cell surface markers. recently, neuraminidase treatment has been shown to uncover receptors for the a hemagglutinin of the snail helix pomatia (hp) on populations (preferentially t cells) of human blood lymphocytes. we have used hp coupled to sepharose in columns to fractionate mouse spleen cells. fractions consisted of passed cells (fraction i) and cells eluted with the competitive hapten n-acetyl-d-gal ...	1978	309820
class-specific antibody titres (elisa) against the primary immunogen helix pomatia haemocyanin (hph) in man.	using the indirect elisa technique, igm, igg and iga antibody titres against the primary test immunogen helix pomatia haemocyanin (hph) could be measured. all twenty-seven normal volunteers (age range 26-74 years) developed maximal or high igm, igg and iga antibody titres 2 weeks after primary immunization with 1.0 mg hph subcutaneously. lower dose immunization resulted in submaximal responses. titre kinetics in the three ig classes correlated in height and time per person. there was no signific ...	1978	80296
adherent cells as targets in mitogenic t-lymphocyte stimulation: replacement of lymphocyte-bound mitogens by nonmitogenic substances.	the sequence of events in mitogenic t-lymphocyte activation was investigated by employing purified mouse peritoneal adherent cells and t-lymphocyte populations. after treatment of adherent cells with the oxidizing agents sodium periodate or neuroaminidase plus galactose oxidase and removal of the mitogens, the cells acquired the ability to stimulate purified t-lymphocytes added subsequently. for this process of t-lymphocyte activation, the additional presence of the membrane interacting substanc ...	1979	226472