Publications

fine structure of trichomitus batrachorum (perty).		1972	4116367
fine-structural changes associated with pseudocyst formation in trichomitus batrachorum.		1973	4709678
trichomonas vaginalis, tritrichomonas foetus, and trichomitus batrachorum: comparative proteolytic activity.	at least four proteolytic activities were detected in the lysates of each of trichomonas vaginalis, tritrichomonas foetus, and trichomitus batrachorum. these were hpaase, a dithiothreitol-dependent activity on hide powder azure; azcase, a dithiothreitol-dependent activity on azocasein; and two distinct activities towards peptide nitroanilide derivatives--one was optimally active at ph 7 and stimulated by dithiothreitol; the other had no dithiothreitol requirement and was highly active at ph 5. h ...	1984	6389167
s-adenosylhomocysteine hydrolase activity in trichomonas vaginalis and other trichomonads.	s-adenosylhomocysteine hydrolase has been detected in crude homogenates of trichomonas vaginalis, tritrichomonas foetus and trichomitus batrachorum at activities of 14, 1.2 and 3.3 nmol min-1 mg-1 protein, respectively. the enzyme from t. vaginalis was found to be soluble with ph optimum of 8.0 and apparent km values for adenosine and homocysteine of 100 and 155 microm, respectively. ara a was shown to inhibit the t. vaginalis enzyme but only at relatively high concentration (i50 100 microm), wh ...	1985	3932851
polyamine biosynthesis in trichomonads.	trichomonas vaginalis, tritrichomonas foetus and trichomitus batrachorum grown in modified diamond's medium all had high concentrations of putrescine and lower concentrations of spermidine and spermine. ornithine decarboxylase (odc; ec 4.1.1.17) was detectable in all three species although at significantly different levels. trichomonas vaginalis had the highest activity (typically around 1.85 nmol min-1 (mg protein)-1), trichomitus batrachorum the lowest (0.11 nmol min-1 (mg protein)-1). the tri ...	1986	3090433
comparative study of ferredoxin-linked and oxygen-metabolizing enzymes of trichomonads.	1. the activities of pyruvate:methyl viologen oxidoreductase (ec 1.2.7.1), hydrogenase (ec 1.18.99.1), nadh:methyl viologen oxidoreductase (ec 1.6.99.3), nadph:methyl viologen oxidoreductase (ec 1.6.99.1), nadh oxidase (ec 1.6.99.3) and nadph oxidase (ec 1.6.99.1) were determined for trichomonas vaginalis, tritrichomonas foetus and trichomitus batrachorum. 2. the three trichomonad species were found to differ significantly, especially with respect to nadh oxidase and nadh:methyl viologen oxidore ...	1987	3497772
l-methionine catabolism in trichomonads.	trichomonas vaginalis growing in complex medium produced volatile thiols at a rate of 0.7 nmol min-1 (mg protein)-1 and the parasite suspended in pbs with l-methionine excreted volatile thiols, including methanethiol, and alpha-keto acid. cell-free extracts of the parasite also produced volatile thiols from l-methionine, at the rate of 5.4 nmol min-1 (mg protein)-1. thiol production was not detectable with living cells or cell-free extracts of tritrichomonas foetus, trichomitus batrachorum or pe ...	1987	3496535
phosphomonoesterases of leishmania mexicana mexicana and other flagellates.	amastigotes and log-phase promastigotes of leishmania mexicana mexicana contained distinct acid phosphatase, 3'-nucleotidase and 5'-nucleotidase activities, distinguishable by their response to ph and inhibitors. both tartrate-sensitive and tartrate-resistant acid phosphatase were present in the two forms, amastigotes possessed less tartrate-resistant acid phosphatase than promastigotes. a tartrate-sensitive acid phosphatase was secreted into the medium in large amounts during the growth in vitr ...	1987	3037369
the use of a highly sensitive electrophoretic method to compare the proteinases of trichomonads.	a highly sensitive electrophoretic method involving gelatin-containing polyacrylamide gels has been used to analyse trichomonad proteinases. multiple forms, optimally active at ph 5-6, were present in all four species examined, but the species could be distinguished from one another by both quantitative and qualitative differences. the intestinal parasites, trichomitus batrachorum and pentatrichomonas hominis, had lower specific activities than the urogenital parasites, trichomonas vaginalis and ...	1987	3302703
actin cytoskeleton demonstration in trichomonas vaginalis and in other trichomonads.	the flagellate form of trichomonas vaginalis (t v) transforms to amoeboid cells upon adherence to converslips. they grow and their nuclei divide without undergoing cytokinesis, yielding giant cells and a monolayer of t v f-actin was demonstrated in trichomonas vaginalis by fluorescence microscopy using phalloidin and an anti-actin mab which labelled the cytoplasm of both the flagellate and amoeboid forms. comparative electrophoresis and immunoblotting established that the actin band has the same ...	1996	9175265
alpha-tubulin from early-diverging eukaryotic lineages and the evolution of the tubulin family.	the tubulin gene family, which includes alpha-,beta-, and gamma-tubulin subfamilies, is composed of highly conserved proteins which are the principle structural and functional components of eukaryotic microtubules. we are interested in (1) establishing when in eukaryotic evolution the duplications leading to paralogous alpha, beta, and gamma subfamilies occurred and (2) the possible utility of tubulin sequences in reconstructing organismal phylogeny. to broaden the taxonomic representation of al ...	1996	8952074
phylogenetic relationships of the glycolytic enzyme, glyceraldehyde-3-phosphate dehydrogenase, from parabasalid flagellates.	over 90% of the open reading frame of gap genes for glycolytic glyceraldehyde-3-phosphate dehydrogenase (gapdh; ec 1.2.1.12) was obtained with pcr from five species of parabasala. with gap1 from trichomonas vaginalis obtained earlier, the data include two sequences each for three species. all sequences were colinear with t. vaginalis gap1 and shared with it as a synapomorphy a 10- to 11-residue insertion not found in any other gap and an s-loop with characteristic features of eubacterial gapdh. ...	1998	9694668
new insights into the phylogeny of trichomonads inferred from small subunit rrna sequences.	small subunit ribosomal dna sequences were obtained by polymerase chain reaction from four trichomonad species: a frog endosymbiont trichomitus batrachorum, an intestinal endosymbiont of a squamate reptile, hypotrichomonas acosta and two free-living isolates, monotrichomonas carabina and monotrichomonas sp. molecular trees inferred by distance, parsimony and likelihood techniques identify three well-resolved clusters within the trichomonads, however bootstrap values do not strongly support a par ...	1998	23194718
molecular phylogeny of parabasalids based on small subunit rrna sequences, with emphasis on the trichomonadinae subfamily.	we determined small subunit ribosomal dna sequences from three parabasalid species, trichomitus batrachorum strain r105, tetratrichomonas gallinarum, and pentatrichomonas hominis belonging to the trichomonadinae subfamily. unrooted molecular phylogenetic trees inferred by distance, parsimony, and likelihood methods reveal four discrete clades among the parabasalids. the trichomonadinae form a robust monophyletic group. within this subfamily t. gallinarum is closely related to trichomonas species ...	2000	10651299
centrin protein and genes in trichomonas vaginalis and close relatives.	anti-centrin monoclonal antibodies 20h5 and 11b2 produced against clamydomononas centrin decorated the group of basal bodies as well as very closely attached structures in all trichomonads studied and in the devescovinids foaina and devescovina. moreover, these antibodies decorated the undulating membrane in trichomonas vaginalis, trichomitus batrachorum, and tritrichomonas foetus, and the cresta in foaina. centrin was not demonstrated in the dividing spindle and paradesmosis. immunogold labelin ...	2000	10750840
phylogenetic relationships of class ii fumarase genes from trichomonad species.	class ii fumarase sequences were obtained by polymerase chain reaction from five trichomonad species. all residues known to be highly conserved in this enzyme were present. nuclear run-on assays showed that one of the two genes identified in tritrichomonas foetus was expressed, whereas no fumarase transcripts were detected in the related species trichomonas vaginalis. these findings corroborate previous biochemical data. fumarase genes were also expressed in monocercomonas sp. and tetratrichomon ...	2001	11470849
molecular phylogeny of trichomonadidae family inferred from its-1, 5.8s rrna and its-2 sequences.	the trichomonads have been the subject of several molecular studies that reported some discrepancies both at the lower and higher taxonomic levels. the purpose of this study was to make an extensive phylogenetic analysis of the trichomonadidae using its-1/5.8s/its-2 sequences, to better understand its phylogeny and the usefulness of this marker. its-1/5.8s/its-2 sequences of 36 strains from 14 species belonging to trichomonadidae and monocercomonadidae were analysed, in which 20 were newly deter ...	2004	15217735
design and validation of an oligonucleotide probe for the detection of protozoa from the order trichomonadida using chromogenic in situ hybridization.	infections with protozoal parasites of the order trichomonadida are often observed in veterinary medicine. based on the trichomonad species involved these infections are either asymptomatic or can lead to sometimes serious disease. to further study protozoal agents of the order trichomonadida the establishment of a method to detect trichomonads directly in the tissue, allowing parasite-lesion correlation, is necessary. here we describe the design and evaluation of an oligonucleotide probe for ch ...	2010	20395049
first evidence of previously undescribed trichomonad species in the intestine of pigs?	three different parasites of the phylum parabasala (tritrichomonas foetus, trichomitus rotunda and tetratrichomonas buttreyi) have been described in pigs. in a previous study (mostegl et al., 2011) approximately 47% of 91 paraffin wax-embedded intestinal samples of pigs which were trichomonas-positive by in situ hybridization using a probe with a broad reactivity spectrum contained other species than t. foetus. out of these, intestinal trichomonads from three pigs (pigs 1-3) were further analyze ...	2011	22093906
new hosts of simplicimonas similis and trichomitus batrachorum identified by 18s ribosomal rna gene sequences.	trichomonads are obligate anaerobes generally found in the digestive and genitourinary tract of domestic animals. in this study, four trichomonad isolates were obtained from carabao, dog, and pig hosts using rectal swab. genomic dna was extracted using chelex method and the 18s rrna gene was successfully amplified through novel sets of primers and undergone dna sequencing. aligned isolate sequences together with retrieved 18s rrna gene sequences of known trichomonads were utilized to generate ph ...	2013	23936631
molecular characterization of trichomonads isolated from animal hosts in the philippines.	trichomonads are amitochondrial anaerobic flagellated protists that are either parasites or commensals, generally living in the digestive or genitourinary tract of humans and animals. it has been reported that these protozoa can migrate to other sites in their target host, can adapt to new hosts, and are capable of zoonotic transmission. in this study, 59 trichomonad isolates from different animal hosts in the philippines were identified and characterized. primer sets were designed and were succ ...	2013	23622817