Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| an epidemic outbreak of canine cutaneous leishmaniasis in colombia caused by leishmania braziliensis and leishmania panamensis. | the largest recorded outbreak of cutaneous leishmaniasis in colombia's history occurred during 2005-2009 in soldiers of the colombian army, with ~40,000 cases. this outbreak was caused by the influx of military personnel into the jungle with the mission of combat illicit crops and the guerrilla. the soldiers remain for long periods within the rainforest and are exposed to the bite of infected sand flies. during the military activities, soldiers work with dogs specially trained to detect landmine ... | 0 | 22556078 |
| the sandfly fauna, anthropophily and the seasonal activities of pintomyia spinicrassa (diptera: psychodidae: phlebotominae) in a focus of cutaneous leishmaniasis in northeastern colombia. | this study was conducted to identify the sandfly fauna and the anthropophilic species in a coffee-growing area of villanueva, norte de santander, colombia, a focus of american cutaneous leishmaniasis, and to analyse the relationship between the most frequent species and rainfall, relative humidity and temperature, with the aim of contributing to epidemiological surveillance in the area. sandfly collections were performed fortnightly between february 2006-september 2007 using automatic light trap ... | 0 | 23778653 |
| detection of leishmania (viannia) in nyssomyia neivai and nyssomyia whitmani by multiplex polymerase chain reaction, in southern brazil. | sandflies transmit pathogens of leishmaniasis. the natural infection of sandflies by leishmania (viannia) was assessed in municipalities, in the state of paraná, in southern brazil. sandflies were collected with falcão and shannon traps. after dissection in search of flagellates in digestive tubes and identification of the species, female sandflies were submitted to the multiplex polymerase chain reaction (multiplex pcr) for detection of the fragment of the kdna of leishmania (viannia) and the f ... | 0 | 25229218 |
| leishmania (viannia) guyanensis infection, austria. | 0 | 22932031 | |
| first report of cutaneous leishmaniasis caused by leishmania (leishmania) infantum chagasi in an urban area of rio de janeiro, brazil. | american tegumentary leishmaniasis (atl) is an infectious disease caused by protozoa of the genus leishmania, and transmitted by sandflies. in the state of rio de janeiro, almost all of the cases of american tegumentary leishmaniasis (atl) are caused by leishmania (viannia) braziliensis, while cases of visceral leishmaniasis (vl) are caused by leishmania (leishmania) infantum chagasi. the resurgence of autochthonous vl cases in rio de janeiro is related to the geographic expansion of the vector ... | 0 | 26603237 |
| the flagellar protein flag1/smp1 is a candidate for leishmania-sand fly interaction. | leishmaniasis is a serious problem that affects mostly poor countries. various species of leishmania are the agents of the disease, which take different clinical manifestations. the parasite is transmitted by sandflies, predominantly from the phlebotomus genus in the old world and lutzomyia in the new world. during development in the gut, leishmania must survive various challenges, which include avoiding being expelled with blood remnants after digestion. it is believed that attachment to the gu ... | 0 | 25793476 |
| aspects of the ecology of phlebotomines (diptera: psychodidae: phlebotominae) in an area of cutaneous leishmaniasis occurrence, municipality of angra dos reis, coast of rio de janeiro state, brazil. | over a complete two-year period, phlebotomine specimens were caught in an area of cutaneous leishmaniasis occurrence in the municipality of angra dos reis. a manual suction tube was used to catch phlebotomines on house walls, and also light traps in domestic and peridomestic settings and in the forest. this yielded 14,170 specimens of 13 species: two in the genus brumptomyia and eleven in the genus lutzomyia. l. intermedia predominantly in domestic and peridomestic settings, with little presence ... | 0 | 24626417 |
| the phlebotomine fauna (diptera: psychodidae) of guaraí, state of tocantins, with an emphasis on the putative vectors of american cutaneous leishmaniasis in rural settlement and periurban areas. | phlebotomine sandflies were captured in rural settlement and periurban areas of the municipality of guaraí in the state of tocantins (to), an endemic area of american cutaneous leishmaniasis (acl). forty-three phlebotomine species were identified, nine of which have already been recognised as acl vectors. eleven species were recorded for the first time in to. nyssomyia whitmani was the most abundant species, followed by evandromyia bourrouli, nyssomyia antunesi and psychodopygus complexus. the s ... | 0 | 23903972 |
| sandflies (diptera: psychodidae) associated with opossum nests at urban sites in southeastern brazil: a risk factor for urban and periurban zoonotic leishmania transmission? | sandflies associated with opossum nests are reported for the first time in the yards of residences located in the urban area of the municipality of monte mor, situated in the metropolitan region of campinas, state of são paulo, brazil. eleven specimens of evandromyia cortelezzii and one of evandromyia lenti were captured in two didelphis albiventris nests. ev. cortelezzii is considered a secondary vector species for the transmission of leishmania (viannia) braziliensis and leishmania (leishmania ... | 0 | 24789554 |
| [preliminary note on the intradermal reaction produced by polysaccharide fraction isolated from culture forms of leishmania brasiliensis in american cutaneous leishmaniasis]. | 1951 | 14850031 | |
| [evidence of the pathogenic power for humans of the leishmania brasiliensis vianna 1911 culture]. | 1951 | 14854496 | |
| [experimental leishmaniasis. ii. behavior of leishmania brasiliensis vianna 1911 in hamsters (cricetus [mesocricetus] auratus waterhouse]. | 1951 | 14860776 | |
| [chemical composition of specific soluble substance of trypanosoma cruzi and leishmania brasiliensis extracted by the fuller method; new culture medium for the growth of these flagellates]. | 1952 | 14944717 | |
| [effects of actinomycin c (bayer sanamycin) on schizotrypanum cruzi and leishmania braziliensis in vitro and leishmania enriettii and trypanosoma venezuelense in vivo]. | 1955 | 13251386 | |
| in vitro studies of the effect of amphotericin b on leishmania brasiliensis. | 1960 | 13702694 | |
| [immunological aspects of leishmania that parasite man, with special reference to leishmania brasiliensis pifanoi, medina & romero, 1957]. | 1960 | 14486555 | |
| [experimental transmission of leishmania braziliensis. ii. transmission of a mexican strain by the bite of phlebotomus iongipalpis and phlebotomus renel]. | 1962 | 14021934 | |
| [experimental transmission of leishmania braziliensis. i. transmission by inoculation of experimentally infected triturates of phlebotomus longipalpis]. | 1962 | 13880144 | |
| [susceptibility of phlebotomus longipalpis and p. renei to infection by leishmania brasiliensis]. | 1962 | 13880145 | |
| differentiation of leishmania brasiliensis from l. mexicana and l. tropica. | 1963 | 14190221 | |
| experimental inoculation of panamanian mammals with leishmania braziliensis. | 1965 | 5857288 | |
| [multiplication of leishmania brasiliensis on hamster kidney cell culture]. | 1966 | 5909623 | |
| physiological studies on the culture form of four strains of leishmania braziliensis. i. nitrogen content, substrate utilization, and effect of metabolic inhibitors on respiration and its relation to infectivity. | 1967 | 6062079 | |
| finestructure of cultured leishmania brasiliensis. | 1969 | 5777447 | |
| comparative experimental infections with costa rican strains of leishmania braziliensis vianna, 1911. | 1969 | 4390862 | |
| metabolism of glucose-c14 in leishmania brasiliensis. | 1969 | 5777371 | |
| leishmania braziliensis isolated from sloths in panama. | two edentates, the twotoed sloth choloepus hoffmanni and the three-toed sloth bradypus infuscatus, infected with leishmania were found in panama. the rates of infection were 14.1 and 1.3 percent in choloepus and bradypus, respectively. leishmania braziliensis sensu lato was cultured from skin, blood, spleen, liver, or bone marrow of 13 sloths often from two or more tissues from the same animal. this strain is indistinguishable from leishmania strains isolated from hunmanis in panama. | 1969 | 5783717 |
| bikaverin, an antibiotic from ibberella fujikoi, effective against leishmania brasiliensis. | 1970 | 5497222 | |
| the effect of palitantin, a metabolite of penicillium frequentans on leishmania brasiliensis. | 1970 | 4315640 | |
| leishmania braziliensis: physical and chemical stress in hamsters. | 1971 | 5545022 | |
| detection of leishmania braziliensis by xenodiagnosis. | 1972 | 4647653 | |
| isolation of leishmania braziliensis by intraperitoneal inoculation of blood from a reservoir host into hamsters. | 1972 | 5048865 | |
| letter: isolation of leishmania braziliensis from a costa rican sandfly and its possible use as a human vaccine. | 1973 | 4778193 | |
| new organelle observed in leishmania brasiliensis. | 1973 | 4777391 | |
| leishmaniasis in brazil. ix. considerations on the leishmania braziliensis complex. importance of sandflies of the genus psychodopygus (mangabeira) in the transmission of l. braziliensis braziliensis in north brazil. | 1973 | 4784055 | |
| onset of espundia after many years of occult infection with leishmania braziliensis. | 1973 | 4200811 | |
| [mucocutaneous lesions produced by leishmania braziliensis]. | 1974 | 4535200 | |
| antileishmanial effect of allopurinol. | allopurinol (4-hydroxypyrazolo[3,4-d]pyrimidine) has been shown to inhibit the growth of leishmania braziliensis in vitro at concentrations which are attainable in human tissues and body fluids. this compound is believed to act by interdicting the de novo synthesis of pyrimidines, probably through the formation of allopurinol ribotide. its lack of toxicity makes it a potential candidate for animal experimentation and it may serve as a prototype for other agents with similar mechanisms of action. | 1974 | 4462464 |
| the surface membrane of leishmania. i. the effects of lectins on different stages of leishmania braziliensis. | the infective stages of leishmania braziliensis, amastigotes and promastigotes subcultured a limited number of times, were agglutinated by ricinus communis agglutinin and concanavalin a. these results suggest that terminal ligands similar or identical with alpha-d mannose, alpha-d glucose (specific receptors for con a), and alpha-d galactose (specific receptor for rca) are present in the surface membrane of l. braziliensis. noninfective promastigotes from the same stock, but subcultured approxim ... | 1975 | 1237556 |
| the isolation of leishmania braziliensis from sloths in costa rica. | eight out of 47 bradypus griseus and 14 out of 18 choloepus hoffmanni yielded positive cultures for flagellates from blood, skin, liver or spleen. in some cases, more than one species of flagellate was isolated from one animal or site. although amastigotes were obtained in tissue cultures from several isolates containing different types of promastigotes, only four of these strains (two from each species of host) were infective for hamsters and considered as leishmania braziliensis. infection wit ... | 1975 | 1155705 |
| [comparative electron microscope studies on the labelling leishmania donovani, leishmania tropica and leishmania braziliensis with ferritin (author's transl)]. | the labelling of negative charges on the cell surface of the developing promastigote forms in leishmania donovani, l. tropica and l. braziliensis was determined using cationized ferritin and electron microscopy. ferritin deposits were seen exclusively on the pellicle, since they cannot penetrate the cell membrane. the ferritin particles were distributed in regular rows covering the whole cell surface. the mean diameter of the particles was 8.76 nm and at a magnification of 104.000 an average of ... | 1975 | 1216327 |
| influence of leishmania brasiliensis on transplantable murine leukemia. | 1976 | 1071169 | |
| [miconazole per os in tinea and deep mycosis]. | for the last 6 years we have been using miconazole cream in the treatment of tinea and vaginal candidiasis. we think that it is at least as good as the best drugs for this purpose, but consider it difficult to decide about its superiority. therefore we refrain from describing this part of our experience in detail. we have employed miconazole "per os" at the daily doses of 24-28 mg./kg. in treating 3 cases of tinea corporis or cruris by "trichophyton rubrum". symptoms ceased in 3 days; mycologic ... | 1976 | 988459 |
| an electron microscopic and cytochemical detection of concanavalin a receptors on the cell membrane of leishmania braziliensis guyanensis. | promastigotes of leishmania braziliensis quyanensis cultivated in the nnn medium agglutinate with concanavalin a (con a). the protozoon was agglutinated at different concentrations of con a. maximal agglutination was obtained with 150 mug/ml. three types of agglutination were observed: flagellar-flagellar, flagellar-body and body-body. cell surface polysaccharides, glycoproteins or glycolipids were demonstrated using the periodic acid-thiosemicarbazide-silver proteinate technic. a con a-horserad ... | 1976 | 961000 |
| natural cutaneous leishmaniasis among dogs in panama. | a search for cutaneous leishmaniasis among dogs was conducted in several forest settlements of central panama from 1968 to 1973. a total of 11 (3.3%) of 333 animals examined was found infected and, in 9 of these, parasites were isolated in culture and characterized as leishmania braziliensis. infected dogs occurred in three settlements, one of which was free of human leishmaniasis. in the latter case, infections were acquired in the nearby forest during hunting excursions. ulcerated skin lesions ... | 1976 | 943961 |
| epidemiological patterns of cutaneous leishmaniasis in panama. i. epidemics among small groups of settlers. | epidemic of cutaneous leishmanniasis among a small group of people established in a panamanian forested area was studied. the first settlers arrived in june 1971, and by june 1973 there were 37 persons, 33 of whom had no previous history of the disease. epidemiological surveys were made in june 1973 and march 1974. the disease developed in 14 (42.4%) of the 33 susceptible persons by june 1973, with an exposure time ranging from one to 14 months. the last case occurred in april 1973, and no new c ... | 1976 | 1267510 |
| letter: metastatic spread of leishmania braziliensis braziliensis to the extremities of hamsters. | 1976 | 1265829 | |
| the action of oxamniquine on leishmania braziliensis braziliensis in hamsters. | 1976 | 1273408 | |
| epidemiological patterns of cutaneous leishmaniasis in panama. ii. incidental occurrence of cases in non-endemic settlements. | epidemiological studies on cutaneous leishmaniasis were carried out in a settlement in the bayano region, republic of panama, during 1971-72. this settlement was established about 12 years earlier for the purpose of cattle raising by small groups of settlers. the primary forest was cut and cleared and the area planted with grass. the presence of active cases of cutaneous leishmaniasis among humans and animals, both domestic and ferals, was investigated. in addition, phlebotomine sandflies were c ... | 1976 | 1267511 |
| epidemiological patterns of cutaneous leishmaniasis in panama. iii. endemic persistence of the disease. | endemic persistence of cutaneous leishmaniasis is described in el aguacate, a community established in the forest of central panama about 75 years ago. physiographic pecularities partially isolate el aguacate from other villages in the region. some of the original forest environment has been preserved and man-biting species of phlebotomine sandflies were abundant as was the two-toed sloth, choloepus hoffmanni, the principal reservoir host of leishmania braziliensis in panama. the inhabitants, as ... | 1976 | 816214 |
| observations on the in vitro cultivation of leishmania braziliensis. | 1977 | 592043 | |
| in vitro infection of murine macrophage by leishmania brasiliensis. mechanism of penetration. | the in vitro infection of peritoneal murine macrophages by a cultured pathogenic strain of leishmania brasiliensis was studied. by phase contrast and light microscopy it could be observed that l. brasiliensis is rapidly endocytosed by the macrophages and localized in the cytoplasm in a vacuole inside which it multiplies. by phase contrast and electron microscopy it was observed that l. brasiliensis enters the macrophage by the anterior end through which it first attaches to the cell membrane. th ... | 1977 | 591111 |
| phosphofructokinase of leishmania donovani and leishmania braziliensis and its role in glycolysis. | it was shown in an investigation of the phosphofructokinases of leishmania donovani and leishmania braziliensis that both enzymes are similar to that of crithidia fasciculata. although the enzymes are allosteric with respect to their substrates and require amp for activation, there is no influence by other heterotropic modifiers. the mg2+-atp chelate activates these enzymes in a first order process and they can be inhibited by free atp. the inhibition is reversed by the activator, amp, in a comp ... | 1977 | 18601 |
| the surface membrane of leishmania. ii. energy dependent agglutination of leishmania braziliensis by concanavalin a. | 1977 | 416634 | |
| muco-cutaneous leishmaniasis: intradermal test with a promastigote suspension and a crude extract from leishmania braziliensis. | 1977 | 613434 | |
| antileishmanial effect of allopurinol. ii. relationship of adenine metabolism in leishmania species to the action of allopurinol. | allopurinol was shown to be effective in vitro against leishmania mexicana and leishmania donovani as well as against leishmania braziliensis. the major metabolic derivative of allopurinol in humans, oxipurinol, also is antileishmanial for l. donovani. the antileishmanial effect of allopurinol and oxipurinol can be specifically reversed by adenine, and its metabolic precursors and derivatives, but by no other purines or their derivative. it is proposed that the adenylosuccinate synthetase or the ... | 1977 | 925380 |
| isolation of leishmania braziliensis from a wild rodent in costa rica. | leishmania braziliensis panamensis has been isolated in culture from one spiny-pocket mouse (heteromys dermarestianus) out of 43 examined, proving that rodents of this genus can be reservoirs of leishmania of the braziliensis complex as well as of mexicana in latin america. | 1977 | 907043 |
| cultivation of leishmania donovani and leishmania braziliensis in defined media: nutritional requirements. | nutritional requirements of promastigotes of leishmania donovani and leishmania braziliensis were studied in modifications of a simple defined culture medium. "continuous growth," considered as propagation through 10 successive passages, was supported by inorganic salts, 14 l-amino acids (arginine, cysteine, glutamine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, threonine, tryptophan, tyrosine, valine), glucose, adenosine, and a mixture of 11 vitamins and related ... | 1977 | 915847 |
| differences in biological characteristics of three leishmania isolates from patients with espundia. | two recent leishmania isolates from nasal lesions involving the mucosa, one from an autochthonous case from texas and one from a case from eastern peru, were compared with a reference strain of leishmania braziliensis from brazil for the biological characteristics which distinguish the l. braziliensis complex from l. mexicana. the characteristics of in vitro growth, pathogenesis in the hamster, and position of the promastigotes in the gut of vector sandflies were determined and compared with the ... | 1977 | 907046 |
| in vitro culture of leishmania brasiliensis in a semi-defined medium. | 1978 | 757727 | |
| purine metabolism in leishmania donovani and leishmania braziliensis. | we have studied purine metabolism in the culture forms of leishmania donovani and leishmania braziliensis. these organisms are incapable of synthesizing purines de novo from glycine, serine, or formate and require an exogenous purine for growth. this requirement is better satisfied by adenosine or hypoxanthine than by guanosine. both adenine and inosine are converted to a common intermediate, hypoxanthine, before transformation to nucleotides. this is due to the activity of an adenine aminohydro ... | 1978 | 719006 |
| leishmania braziliensis: dissemination of panamanian strains in golden hamsters. | 1979 | 510440 | |
| the effect of peritoneal macrophages from mice injected with leishmania braziliensis on the in vitro growth of tumor cells. | leishmania braziliensis reportedly is capable of producing a reduction in the growth of solid and ascites murine leukemia and lymphomas. the possibility that the inhibitory effect on the ascites tumor was produced by the activation of the macrophage peritoneal cell population was explored. it was observed that adherent cells from the peritoneal cavity of l. braziliensis injected mice caused a marked inhibitory effect on the in vitro growth of the 6c3hed lymphoma and el-4 leukemia cells. this eff ... | 1979 | 489060 |
| metabolism of pyrazolo(3,4-d)pyrimidines in leishmania braziliensis and leishmania donovani. allopurinol, oxipurinol, and 4-aminopyrazolo(3,4-d)pyrimidine. | leishmania donovani and leishmania braziliensis grown in culture formed millimolar concentrations of allopurinol ribonucleoside 5'-monophosphate from [6-14c]allopurinol. in addition, allopurinol 1-ribonucleoside, oxipurinol riboside 5'-monophosphate, and three new metabolites of allopurinol, namely, 4-aminopyrazolo(3,4-d)pyrimidine ribonucleoside 5'-monophosphate and the corresponding di- and triphosphates (1-ribosyl 4-aminopyrazolo(3,4-d)pyrimidine 5'-diphosphate and 1-ribosyl 4-aminopyrazolo(3 ... | 1979 | 438170 |
| leishmania braziliensis and leishmania mexicana: experimental cutaneous infections in golden hamsters. | 1979 | 437020 | |
| the noguchi-adler phenomenon: an ultrastructural study of the effects of homologous antiserum on the growth of promastigotes of leishmania braziliensis braziliensis and leishmania hertigi hertigi. | a light and electron microscope study was made of the agglutinated bodies formed on growing promastigotes of a leishmania b braziliensis and a l. h. hertigi strain in their homologous antisera to determine the role of leishmanial excreted factor (ef) in the noguchi-adler phenomenon, since the l. b. braziliensis strain produced a demonstrable ef, whereas the l. h. hertigi strain did not. promastigotes of l. b. braziliensis, when grown in high concentrations of homologous antiserum, formed noguchi ... | 1979 | 395912 |
| leishmania in phlebotomid sandflies. vii. on the taxonomic status of leishmania peruviana, causative agent of peruvian 'uta', as indicated by its development in the sandfly, lutzomyia longipalpis. | the name leishmania peruviana was given by velez (1913) to the parasite responsible for a form of cutaneous leishmaniasis known as 'uta'; this disease occurs in the peruvian andes. clinical similarities between uta and 'oriental sore', which is caused by leishmania tropica of the eastern hemisphere, have, however, led to the suggestion that uta is simply due to l. tropica, which was introduced into latin america by african slaves or european immigrants. leishmania species are divisible into thre ... | 1979 | 43976 |
| quantitative estimation of leishmanial antibody titers by enzyme-linked immunosorbent assay. | the response to immunization against leishmania braziliensis in rabbits was followed by measurement of antibody by enzyme-linked immunosorbent assay (elisa), passive hemagglutination, complement fixation, and countercurrent immunoelectrophoresis. passive hemagglutination and complement-fixation titers were interpreted according to standard methods, whereas elisa exact titers were derived by the equation y = a-b log x, which describes the straight line that results when absorbances are plotted ag ... | 1979 | 387886 |
| inhibition of growth and purine-metabolizing enzymes of trypanosomid flagellates by n6-methyladenine. | n6-methyladenine (6-methylaminopurine [6-ma]), a plant growth regulator and a normal constituent of nucleic acids, has been found to inhibit the growth of trypanosoma cruzi, leishmania braziliensis, l. donovani, l. tarentolae, l. mexicana, and crithidia fasciculata. the extent of growth inhibition in these organisms is related to the sensitivity of guanine deaminase (guanine aminohydrolase, ec 3.5.4.3), adenine deaminase (adenine aminohydrolase, ec 3.5.4.2), and adenosine hydrolase and phosphory ... | 1980 | 6994636 |
| a new method for the partial purification of leishmania amastigotes from cutaneous lesions. | amastigotes from leishmania braziliensis and l. mexicana were isolated from cutaneous lesions in infected animals using the plant lectin concanavalin a as a specific agglutination agent. amastigotes were collected in preparations of up to 95% purity as determined by cell count. the parasites obtained by this method showed no apparent loss of viability as measured by growth rated and dna replication, or pathogenicity as measured by routine passages in hamsters or mice. | 1980 | 6992056 |
| leishmania braziliensis braziliensis: metastatic infection in a golden hamster. | 1980 | 7210144 | |
| leishmania donovani and leishmania braziliensis: hexokinase, glucose 6-phosphate dehydrogenase, and pentose phosphate shunt activity. | 1980 | 7349998 | |
| characterization of leishmania spp. by isozyme electrophoresis. | in this study, isozyme patterns for 14 different enzymes were compared for culture strains of leishmania braziliensis, l. hertigi, l. mexicana, l. donovani, l. tropica, and l. adleri. the isozyme separation was made by means of cellulose acetate electrophoresis. each of the species had distinct isozyme patterns for aspartate aminotransferase, glucose 6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, and fructokinase. for other enzymes, two or more species had identically migrating ban ... | 1980 | 7369438 |
| experimental infection of mystromys albicaudatus with leishmania braziliensis: pathology. | thirty-four mystromys albicaudatus were injected intradermally with leishmania braziliensis promastigotes and examined and killed during a 12-week period. all animals developed single cutaneous lesions at the sites of inoculation that began as a papular thickening in the dermis and progressed to a 2.0 cm crateriform ulcer. the histopathology of these lesions was characterized by granuloma formation and diffuse infiltrates of lymphocytes and plasma cells. parasites were most frequently observed i ... | 1980 | 7435783 |
| production and secretion of leishmania braziliensis proteins. | the kinetics of secretion of proteins by leishmania braziliensis was followed by incorporation of [3h]leucine into macromolecules produced by the cells which are released into the growth medium. about 10% of the total protein synthesized by actively growing cells is secreted. cycloheximide (100 microgram/ml) and puromycin (0.5 mm) inhibited the incorporation of labelled leucine by 85 and 99%, respectively. the secreted proteins do not seem to result from cell lysis since, first, the kinetics of ... | 1980 | 7442714 |
| leishmania braziliensis in the panamanian two-toed sloth, choloepus hoffmanni. | a total of 498 two-toed sloths, choloepus hoffmanni, collected in central panama was examined for leishmania braziliensis over a 10-year period. isolations of the parasite from 96 (19.3%) of the animals were confirmed by culture and inoculation of golden hamsters. improved culture techniques developed toward the end of the study assisted in determining a greater prevalence of the disease. infectins were completely cryptic in all animals, and the parasite was isolated from skin, blood, liver, spl ... | 1980 | 7446811 |
| micro enzyme-linked immunosorbent assay (elisa) for the serodiagnosis of new world leishmaniasis. | of 21 confirmed cases of new world leishmaniasis, 16 exhibited antibody to antigens of the promastigote of leishmania braziliensis panamensis by the enzyme-linked immunosorbent assay (elisa). comparison of antibody titers obtained by elisa with titers obtained by indirect immunofluorescence using an amastigote substrate confirmed that the sensitivities of the two techniques were within the same range (r = 0.80). although sera from patients with new world leishmaniasis failed to react with antige ... | 1980 | 6154426 |
| [vaccination with leishmania hertigi against the subsequent infection with leishmania mexicana and leishmania braziliensis in hamsters]. | 1980 | 6782641 | |
| immunoglobulin g and immunoglobulin m polar staining of toxoplasma gondii in the indirect immunofluorescence test. | polar staining (ps) of toxoplasma gondii in the indirect immunofluorescence test has been considered a nonspecific reaction caused exclusively by certain immunoglobulin m (igm) antibodies and confined to the anterior end of the parasite. after we observed a patient with positive serology for chagas' disease who presented an igg ps reaction, we studied sera from 120 patients with chagas' disease, 20 sera from patients with leishmania donovani infection, and 30 sera from patients with leishmania b ... | 1980 | 6796601 |
| susceptibility of aotus trivirgatus to leishmania braziliensis and l. mexicana. | two aotus trivirgatus (owl monkeys) were infected experimentally with leishmania braziliensis and two with l. mexicana strains of panamanian origin in a pilot study to determine the susceptibility and the course of infection of cutaneous leishmaniasis in this primate species. montenegro skin tests performed on all animals prior to parasite inoculation were negative. a standardized inoculum of promastigotes was injected intradermally on the nose of each monkey. all of the animals developed infect ... | 1981 | 7212172 |
| the in vitro cultivation of members of the leishmania braziliensis complex. | 1981 | 7268844 | |
| leishmaniasis in brazil: xvi. isolation and identification of leishmania species from sandflies, wild mammals and man in north para state, with particular reference to l. braziliensis guyanensis causative agent of "pian-bois". | a total of 125 wild mammals (14 different species) were examined for evidence of infection with leishmania in an area of primary forest highly endemic for "pian-bois", due to leishmania braziliensis guyanensis, in north pará state, brazil. parasites isolated were characterized biologically, and biochemically on enzymic profiles. l. b. guyanensis was isolated from the viscera of one lesser anteater (tamandua tetradactyla) and one opossum (didelphis marsupialis), and the skin of one rodent (proech ... | 1981 | 7324128 |
| the opossum, didelphis marsupialis (marsupialia: didelphidae), as a reservoir host of leishmania braziliensis guyanensis in the amazon basin of brazil. | a total of 52 opossums (six species) were examined for evidence of infection with leishmania in three different areas of forest near manaus, amazonas state, brazil. no infections were detected in 27 opossums from a region of relatively undisturbed forest, including specimens of didelphis marsupialis (18); metachirus nudicaudatus (four); monodelphis brevicaudata (one); marmosa cinerea (two); m. murina (one) and m. parvidens (one). of 15 d. marsupialis captured from a biological reserve, much dist ... | 1981 | 7324129 |
| the importance of edentates (sloths and anteaters) as primary reservoirs of leishmania braziliensis guyanensis, causative agent of "pianbois" in north brazil. | 1981 | 7324143 | |
| ribosomal dna of leishmania brasiliensis: number of ribosomal copies and gene isolation. | the dna content of the hemoflagellate leishmania brasiliensis, strain y, has been determined by colorimetric reactions and found to be nearly 0.226 pg/cell. when this dna is bound to filters and hybridized with labeled rrna from the same organism, saturation is reached at 0.47% of the dna, corresponding to an estimated 160 ribosomal gene copies. when the dna is sheared and centrifuged to equilibrium in cscl gradients, two major satellites of the main band (rho = 1.712 g/cm3) are observed: a heav ... | 1982 | 7131353 |
| monoclonal antibodies that distinguish subspecies of leishmania braziliensis. | 1982 | 7108210 | |
| the specificity of purine base and nucleoside uptake in promastigotes of leishmania braziliensis panamensis. | promastigotes of leishmania braziliensis panamensis absorbed the purines adenine, hypoxanthine, adenosine and inosine by a combination of diffusion and mediated components. when the uptake rates for these substrates were corrected for diffusion and compared, the purine bases adenine and hypoxanthine were transported at a significantly slower rate than the purine nucleosides adenosine and inosine. competitive interactions among those purines tested confirmed the presence of mediated and diffusion ... | 1982 | 7145470 |
| the tree-buttress biotope: a pathobiocenose of leishmania braziliensis. | a total of 52,033 sandflies, comprising 33 species, was collected in tree buttresses in panama. of the 18,025 females collected, 1,592 (8.8%) engorged phlebotomines of 18 species contained sufficient blood for identification of their vertebrate hosts by the microcapillary precipitin method. lutzomyia ylephiletor and lu. trapidoi, vectors of leishmania braziliensis, had fed predominantly on sloths (47.0% and 65.1%, respectively). lutzomyia shannoni, a common non-anthropophilic sandfly in tree but ... | 1982 | 7072887 |
| interactions between macrophagelike cells and leishmania braziliensis in vitro. | the interaction between macrophagelike cells (p388d murine tumor cells) and leishmania braziliensis panamensis promastigotes was studied in vitro under various conditions by light and electron microscopy. when the macrophagelike cells and l braziliensis promastigate were incubated together for 2 minutes, 90% of the promastigotes were attached to the macrophagelike cells by the tip of the flagella. the macrophagelike cells did not form pseudopods or aggregated microfilaments around the inserted f ... | 1982 | 7091302 |
| vaccination of the african white-tailed rat, mystromys albacaudatus, with sonicated leishmania braziliensis panamensis promastigotes. | the usefulness of sonicated leishmania braziliensis panamensis promastigotes for vaccination was evaluated in mystromys albacaudatus, the african white-tailed rat. thirty-two animals received three intradermal injections of 2 x 10(6) sonicated promastigotes derived from l. braziliensis panamensis at weekly intervals. one month after completion of the immunization schedule, the experimental group of animals was challenged in vivo with 2 x 10(6) live l. braziliensis panamensis promastogotes. at th ... | 1982 | 7072888 |
| cutaneous leishmaniasis in mice: resistance to glucan immunotherapy, either alone or combined with chemotherapy. | leishmania braziliensis, l. mexicana, and l. garnhami were studied for their ability to produce american cutaneous leishmaniasis, using c57bl/6 female mice as the animal model. no significant difference in the clinical course of mouse foot pad infection was found between the three american leishmania species studied. in general, the incubation period varied from 2-4 weeks. mice developed only local swelling and sometimes ulceration at the sites of inoculation. after 4 weeks of progress lesions b ... | 1982 | 7058978 |
| epidemiology of leishmaniasis in french guiana. | lutzomyia umbratilis is confirmed as the vector of leishmania braziliensis guyanensis, the cause of "pian bois" in man in french guiana. although spending most of the year in the forest canopy, this sandfly is abundant at ground level for about two weeks at the beginning of the long rainy season. the maximum number of infective bites per man per hour (3.9) was reached at the end of november 1979. infections were found in one of seven potos flavus, seven of 15 choleopus didactylus and two of 19 r ... | 1982 | 7080142 |
| peptone-yeast autolysate-fetal bovine serum 10, a simple, inexpensive liquid medium for cultivation of leishmania spp. | a simple liquid medium for the cultivation of leishmania parasites is described. leishmania brasiliensis and leishmania peruviana cultured in this medium reached cell densities greater than 10(7) promastigotes per ml within 7 days. this medium compares very favorably with the more complex media used to cultivate leishmania spp. and other hemoflagellates. | 1982 | 7047559 |
| [experimental chemotherapy in hamsters, using paromomycin against 2 isolates of leishmania mexicana and leishmania braziliensis]. | 1982 | 6750713 | |
| hosts of sandfly vectors of leishmania braziliensis guyanensis in the central amazon of brazil. | the blood meals of 2,569 phlebotomine sandflies from areas endemic for cutaneous leishmaniasis in the central amazon of brazil were tested by the microcapillary precipitin method to determine their vertebrate hosts. the two-toed sloth, choloepus didactylus, was the predominant host of two incriminated vectors of leishmania braziliensis guyanensis in the region, lutzomyia umbratilis and lu. anduzei (64.0% and 63.6%, respectively). the lu. "shannoni" group, a complex of several species in which fe ... | 1982 | 7072886 |
| comparative effects of 4-aminopyrazolopyrimidine, its 2'-deoxyriboside derivative, and allopurinol on in vitro growth of american leishmania species. | when assayed on promastigotes of 10 american leishmania isolates (including leishmania brasiliensis and mexicana species), 4-aminopyrazolopyrimidine (aap) was severalfold more active than allopurinol as a leishmanistatic drug. there were some intraspecific and interspecific differences among the isolates in their susceptibility to the inhibitory effects of app and allopurinol. app-2'-deoxyriboside did not affect the 10 isolates tested. this was surprising, because allopurinol riboside has previo ... | 1982 | 6982678 |
| leishmaniasis in brazil: xvii. enzymic characterization of a leishmania from the armadillo, dasypus novemcinctus (edentata), from pará state. | a comparison of enzyme profiles, by starch-gel electrophoresis, has distinguished a leishmania of armadillos (dasypus novemcinctus), from pará state, north brazil, from leishmania braziliensis braziliensis, l. braziliensis guyanensis, l. mexicana amazonensis, l. donovani sensu lato (from bahia, brazil), and l. hertigi deanei. the parasite was separated from l. b. braziliensis and l. b. guyanensis by 8 of the 14 enzymes used (asat, alat, pgm, gpi, g6pd, pep, mpi and gd), although differences in t ... | 1982 | 7164148 |
| [isolation and purification of leishmania braziliensis amastigotes: use in the comparative study of the cellular surface of amastigotes and promastigotes of the nr strain]. | 1982 | 7184282 | |
| leishmania braziliensis: effects of bacteria (staphylococcus aureus and pasteurella multocida) on the developing cutaneous leishmaniasis lesion in the golden hamster. | experimentally induced lesions of cutaneous leishmaniasis and the effect of concurrent bacterial infection on the development of these lesions were studied in the golden hamster. male outbred golden hamsters received intradermal injections at the base of the tail with approximately 10(7) promastigotes of leishmania braziliensis panamensis, or promastigotes combined with staphylococcus aureus or pasteurella multocida or both, bacteria only, or sterile eagle's minimal essential medium (meme). the ... | 1983 | 6873221 |
| leishmaniasis affecting the eyelids. | leishman-donovan bodies were recognized in the smear of a biopsy specimen from an eyelid ulcer. the infecting organisms were identified serologically as leishmania braziliensis panamensis. the ulcer responded to pentavalent antimony. ultrastructurally, the organisms had double-unit membranes, beneath which lay a palisade of microtubules. at one end of the organism, there was a rudimentary flagellum; at the other, the nucleus. a kinetoplast basal complex separated the two. | 1983 | 6849660 |