Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| physiological and dna characterization of candida maltosa, a hydrocarbon-utilizing yeast. | selected yeast classified as candida sake van uden et buckley were examined for their physiological, morphological and immunological properties and their dna relatedness. candida maltosa komagata, nakase et katsuya is herein recognized as a species separate from c. sake, candida maltosa was distinguished from c. sake and from c. tropicalis by insignificant dna reassociation. in addition, c. maltosa was distinguished from c. sake by its higher maximal growth temperature and lower guanine plus cyt ... | 1975 | 53037 |
| accumulation and metabolism of uneven fatty acids present in single cell protein. | liquipron and toprina, obtained by growing yeasts (candida maltosa and candida lipolytica) on n-hydrocarbons, were investigated to ascertain the biological significance and possible toxicological implications of their high content of uneven fatty acids (ufa). it was confirmed that the extent to which ufa accumulate in adipose tissue of rats fed the 2 products reflects only partially their ufa contents. the presence of ufa in rat tissues does not appear to alter intermediate metabolism. the capac ... | 1980 | 7466851 |
| long term toxicity and carcinogenicity of a new protein source in rats. | sprague dawley rats were fed with yeast (candida maltosa) obtained by fermentation of n-paraffins f.u. grade (c12-c19). the yeast was incorporated in the diet at 7.2, 18.4 and 34.5% by weight. each diet was isocaloric and isoproteic with the others and with the standard diet. the yeast supplied 20, 50, 80% of the proteins of the diet respectively. 65 rats per sex per group were selected at random from over 1000 rats and assigned to each of the 4 diets for the carcinogenicity study; 57 rats per s ... | 1981 | 7330873 |
| cloning of a leu gene and an ars site of candida maltosa. | gene libraries of dna from an n-alkane-assimilating yeast strain, candida maltosa iam12247, were constructed, using escherichia coli plasmid vector pbr322. a leu gene from c. maltosa was cloned, and found to complement leu- mutations in e. coli and saccharomyces cerevisiae. in e. coli, the leu gene in the cloned yeast dna fragment was efficiently expressed when inserted into the vector in one orientation, while in the other orientation, it was expressed only weakly. in s. cerevisiae, the candida ... | 1983 | 6315533 |
| increase of translatable mrna for major microsomal proteins in n-alkane-grown candida maltosa. | in an n-alkane-assimilating candida sp., transfer from glucose- to n-alkane-containing medium induced changes in the microsomal proteins, and several distinctive polypeptides were demonstrated in the solubilized microsomal fraction derived from n-alkane-grown cells. long-term-labeling and pulse-labeling experiments in vivo demonstrated the synthesis of the specific microsomal polypeptides. the polypeptides were synthesized as in vitro translation products directed by polyadenylated rna extracted ... | 1984 | 6501225 |
| mode of action of glyphosate in candida maltosa. | the broad-spectrum herbicide glyphosate inhibits the growth of candida maltosa and causes the accumulation of shikimic acid and shikimate-3-phosphate. glyphosate is a potent inhibitor of three enzymes of aromatic amino acid biosynthesis in this yeast. in relation to tyrosine-sensitive 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase and dehydroquinate synthase, the inhibitory effect appears at concentrations in the mm range, but 5-enolpyruvylshikimate 3-phosphate (epsp) synthase is inhibited ... | 1984 | 6152388 |
| absolute dependence of phenylalanine and tyrosine biosynthetic enzyme on tryptophan in candida maltosa. | candida maltosa synthesizes phenylalanine and tyrosine only via phenylpyruvate and p-hydroxyphenylpyruvate. tryptophan is absolutely necessary for the enzymatic reaction of chorismate mutase and prephenate dehydrogenase; activity of prephenate dehydratase can be increased 2.5-fold in the presence of tryptophan. activation of the chorismate mutase, prephenate dehydratase and prephenate dehydrogenase by tryptophan is competitive with respect to chorismate and prephenate with ka 0.06mm, 0.56mm and ... | 1984 | 6479898 |
| regulatory properties of 3-deoxy-d-arabino-heptulosonate-7-phosphate synthase isozymes from candida maltosa. | 1985 | 2860237 | |
| transformation of candida maltosa and pichia guilliermondii by a plasmid containing saccharomyces cerevisiae arg4 dna. | saccharomyces cerevisiae, candida maltosa and pichia guilliermondii have been transformed by the plasmid pye(arg4)411, which contains the s. cerevisiae arg4 gene inserted into pbr322. in all transformants argininosuccinate lyase as well as beta-lactamase were detected. the arg+ phenotype of transformants is mitotically unstable. closed circular pye(arg4)411 dna was detected in transformant dna preparations by hybridization to pbr322 dna and by transformation of e. coli to ampicillin resistance. | 1985 | 3916721 |
| [isolation of a yeast cell-agglutinating component from normal serum by cell column chromatography]. | by cell column chromatography a fraction of porcine serum has been obtained that specifically reacts to the surfaces of the yeast cells of saccharomyces cerevisiae h155. this serum fraction agglutinates only the cells of saccharomyces cerevisiae h155 but does not those of candida maltosa eh15 and yarrowia lipolytica eh59, respectively. | 1985 | 3936917 |
| a comparative immunological investigation of the alkane hydroxylating cytochrome p-450 from the yeast candida maltosa. | the immunological relations of the cytochrome p-450 from the n-alkane utilizing yeast candida maltosa to cytochrome p-450 forms of other organisms - yeasts, bacteria and mammalia - were investigated using a solid-phase double-antibody radioimmunoassay. only the microsomal fraction of other n-alkane utilizing yeasts shows a distinct cross-reaction with an antiserum against cytochrome p-450 from candida maltosa. neither the tested bacterial nor the mammalian cytochromes p-450 cross-react with the ... | 1985 | 4004862 |
| [new yeast vectors containing the autonomously replicating sequences from candida maltosa genome]. | two different dna sequences from the yeast candida maltosa confer the ability to replicate autonomously to the yeast integrative vector pld700 on which they are cloned. the recombinant plasmids pld701 and pld702 with autonomously replicating sequences (ars) from candida maltosa and leu2 gene from saccharomyces cerevisiae transform the auxotrophic strain s. cerevisiae dc5 with the efficiency 3-5 x 10(3) per microgram of dna. like other yeast vectors harbouring ars, these plasmids are not stable i ... | 1985 | 3025715 |
| "natural antibodies" against yeast--a flow-microfluorometric approach. | fluorescent labeling of antibodies in connection with flow-microfluorometry was applied to "natural antibodies" against yeast strains candida maltosa and yarrowia lipolytica, obtained by cell column chromatography. it is shown that "natural antibodies" against these yeast strains react in a specific manner with the surface of the cells. | 1985 | 3161312 |
| construction of a host-vector system in candida maltosa by using an ars site isolated from its genome. | to construct a host-vector system in an n-alkane-assimilating yeast, candida maltosa, the isolation of an ars site from its genome which replicates autonomously in c. maltosa was attempted. leu- mutants of c. maltosa were transformed with a gene library prepared by using yep13 (leu2+) as a vector, and leu+ transformants were obtained at a high frequency. a plasmid named pcs1 was isolated from the recipient cells. pcs1 contained a 6.3-kilobase (kb) fragment of the c. maltosa genome, and a 3.8-kb ... | 1986 | 3015879 |
| n-alkanes induce the synthesis of cytochrome p-450 mrna in candida maltosa. | in the yeast candida maltosa the level of cytochrome p-450 was 100-300-fold higher in alkane-grown cells than in glucose-grown ones (detected by a radioimmunoassay). it was shown immunochemically (1) that this was not the result of an assembly of preexisting apoenzyme with the prosthetic heme group. by cell-free translation of total poly(a)rna in a wheat germ system and subsequent immunoprecipitation it was shown that the amount of mrna coding for cytochrome p-450 paralleled its concentration in ... | 1986 | 2424439 |
| physical mapping and genome organization of mitochondrial dna from candida maltosa. | mitochondrial (mt) dna of the ascomycetous yeast candida maltosa was isolated and characterized. the mtdna is circular and the size estimated from restriction analysis performed with 7 endonucleases was 52 kb pairs. a restriction map was constructed, using the cleavage data of four endonucleases. using mt genes from saccharomyces cerevisiae, six structural genes (large rrna, apocytochrome b, cytochrome c oxidase subunit i and subunit ii, atpase subunit 6 and subunit 9) were located on the c. mal ... | 1986 | 2832075 |
| cloning in saccharomyces cerevisiae of a cycloheximide resistance gene from the candida maltosa genome which modifies ribosomes. | we have previously shown that cycloheximide resistance can be induced in a strain of candida maltosa by modifying ribosomes (m. takagi, s. kawai, y. takata, n. tanaka, m. sunairi, m. miyazaki, and k. yano, j. gen. appl. microbiol. 31:267-275, 1985). the present paper describes the cloning of the gene involved in this resistance (designated rim-c for ribosome modification by cycloheximide) by using a host-vector system of saccharomyces cerevisiae. | 1986 | 3531179 |
| [isolation of surface antigens of candida maltosa responsible for candida-sensitization and their immunochemical characteristics]. | surface antigens from candida maltosa were shown to be of heterogeneous nature while common component was present in all the preparations. these antigens were able to induce both the slow type hypersensitivity and the reaction related apparently to the later phase of immediate hypersensitivity. hypersensitivity of slow and immediate types developed in response to invasion of living culture of c. maltosa a strain. the data obtained suggest that surface antigens from candida might be used for prod ... | 1987 | 3116768 |
| identification of a lys2 mutant of candida maltosa by means of transformation. | we have isolated five mutants of candida maltos, which lack the 2-aminoadipate reductase activity, an enzyme involved in the lysine biosynthesis. by means of complementation analysis using protoplast fusion, the isolated mutants were divided into two complementation groups. thereof the c. maltosa strain g457 could be transformed by the plasmids pdp12 and pdp13, which contain the lys2-coding gene of saccharomyces cerevisiae. on the basis of our presented results obtained by studies on hybridizati ... | 1987 | 2836078 |
| nucleotide sequencing analysis of a leu gene of candida maltosa which complements leub mutation of escherichia coli and leu2 mutation of saccharomyces cerevisiae. | the expression of a leu gene from candida maltosa (designated as c-leu2) isolated previously (kawamura et al. 1983) was shown to be regulated, when transferred into saccharomyces cerevisiae, by leucine and threonine in the medium, as in the case of leu2 gene of s. cerevisiae. the coding region together with the regulatory region was subcloned and the nucleotide sequence was determined. when the sequence of the coding region was compared with that of leu2, the homology was 72% for base pairs and ... | 1987 | 2897248 |
| [chromosome stability in saccharomycete yeasts]. | mutants with high instability of chromosome iii designated chl+ (chromosome loss) were obtained after irradiation with uv the z4221-3c1 haploid disomic for chromosome iii. the chl+ mutants can be divided into two classes: 1) cl2, cl3, cl7, cl9, cl11, cl12, cl13 with elevated level of spontaneous inter- and intragenic recombination; 2) cl4, cl8 which unstable maintenance of chromosome iii not accompanied with elevation of mitotic recombination frequency. the cl4 and cl8 mutants also reveal, in co ... | 1987 | 3326785 |
| complete nucleotide sequence of the peroxisomal acyl coa oxidase from the alkane-utilizing yeast candida maltosa. | 1988 | 3340538 | |
| [study of the structure of mannans from candida maltosa and candida tropicalis using 13c-nmr spectroscopy]. | mannans were isolated from six candida strains and characterized. 13c-nmr spectroscopy revealed interspecific and interstrain difference of the yeasts in the structure of their mannans. | 1988 | 3387383 |
| post-translational transport of proteins into microsomal membranes of candida maltosa. | we have isolated from the yeast candida maltosa microsomal membranes that are active in the translocation of proteins synthesized in cell-free systems derived from c. maltosa, saccharomyces cerevisiae or wheat germ. translocation and core glycosylation of prepro-alpha-factor, a secretory protein, were observed with yeast microsomes added during or after translation. the signal peptide is cleaved off. cytochrome p-450 from c. maltosa, the first integral membrane protein studied in a yeast system, ... | 1988 | 3169003 |
| construction of promoter-probe vectors for candida maltosa, a n-alkane-assimilating yeast, using the leu2 gene of saccharomyces cerevisiae. | for the purpose of isolation of promoter regions which are regulated by a carbon source in the medium in an n-alkane-assimilating yeast, candida maltosa, two promoter-probe vectors were constructed. each of them consists of the leu2 gene of saccharomyces cerevisiae whose 5'-noncoding region was trimmed with bal31, an autonomously replicating sequence isolated from c. maltosa genome (the tra region) which we have previously isolated, and the pbr322 sequence. one of them, pplc2, having the tata bo ... | 1988 | 3068352 |
| [use of the coagglutination reaction of yeast-like candida maltosa fungi for detecting fimbriae in intestinal bacteria]. | the capacity of nonpathogenic yeast-like c. maltosa strains to coagglutinate escherichia coli has been studied. c. maltosa cells have also been shown to coagglutinate e. coli possessing mannose-sensitive adhesins in a wide range of their concentrations (5-140 bacterial cells per c. maltosa cell). strains belonging to types cfa/i and cfa/ii with fimbriae, similarly to their corresponding paired genetically related strains without these adhesins, are practically incapable of agglutinating c. malto ... | 1988 | 2896417 |
| [an evaluation of the degree of serological affinity of the surface structures in candida maltosa and candida albicans]. | the complex preparation of surface antigens was obtained by the treatment of c. maltosa whole cells with beta-mercaptoethanol and their separation into 8 fractions by means of ion exchange chromatography on deae cellulose. the sensitizing capacity of these fractions was studied in the allergic dermal test on guinea pigs and their immunochemical activity, in the immunodiffusion test with homologous antiserum and with the gamma-globulin fraction of antiserum to c. albicans. all fractions induced d ... | 1989 | 2481911 |
| cyclic amp, fructose-2,6-bisphosphate and catabolite inactivation of enzymes in the hydrocarbon-assimilating yeast candida maltosa. | the inactivation of fructose-1,6-bisphosphatase, isocitrate lyase and cytoplasmic malate dehydrogenase in candida maltosa was found to occur after the addition of glucose to starved cells. the concentration of cyclic amp and fructose-2,6-bisphosphate increased drastically within 30 s when glucose was added to the intact cells of this yeast. from these results it was concluded that catabolite inactivation, with participation of cyclic amp and fructose-2,6-bisphosphate, is an important control mec ... | 1989 | 2549901 |
| an improved host-vector system for candida maltosa using a gene isolated from its genome that complements the his5 mutation of saccharomyces cerevisiae. | the host-vector system of an n-alkane-assimilating-yeast, candida maltosa, which we previously constructed using an autonomously replicating sequence (ars) region isolated from the genome of this yeast, utilizes c. maltosa j288 (leu2-) as a host. as this host had a serious growth defect on n-alkane, we developed an improved host-vector system using c. maltosa ch1 (his-) as host. the vectors were constructed with the candida ars region and a dna fragment isolated from the genome of c. maltosa. si ... | 1989 | 2697466 |
| molecular cloning and characterization of the primary structure of the alkane hydroxylating cytochrome p-450 from the yeast candida maltosa. | a cdna library was established starting from poly(a) rna of n-alkane-grown candida maltosa cells and cdna clones were isolated containing the entire coding sequence for the alkane hydroxylating cytochrome p-450. the deduced protein consists of 521 amino acids, contains two putative transmembrane segments in the n-terminal region and has a characteristic heme-binding sequence in the c-terminal part. sequence alignments with members of 11 reported cytochrome p-450 families revealed a strong homolo ... | 1989 | 2735924 |
| long-chain alcohol production by yeasts. | fourteen yeast strains from six genera were analysed for the presence of long-chain alcohols, the highest levels being found in candida albicans. the major alcohols synthesized were saturated, primary alcohols with c14, c16 or c18 chain length with relative proportions of c16 greater than c18 greater than c14. in c. albicans synthesis of long-chain alcohols occurred only after the end of exponential growth. long-chain alcohol contents were lower in organisms grown aerobically as compared with an ... | 1989 | 2750314 |
| [immunochemical study of surface glycoconjugates of yeast-like fungus of the candida genus]. | two complex preparations (extract i and extract ii) of surface antigens were obtained from the yeast-like fungus candida maltosa by treatment of intact cells with beta-mercaptoethylamine and pronase. immune diffusion in agar gel revealed antigenic heterogeneity of the preparations. both of the extracts were found to have at least 3 antigenic components. the extracts induce hypersensitivity of intact guinea pigs and candida sensitization on the 12th day after the injection of candida maltose inta ... | 1989 | 2762258 |
| [activities of gluconeogenetic enzymes in the yeast candida maltosa during growth on glucose or ethanol]. | the activities of fructose-1,6-bisphosphatase, malate dehydrogenase and pep carboxykinase were tested during discontinuous growth of the n-alkane-assimilating yeast candida maltosa on glucose or ethanol. as expected, the highest activities of the enzymes were measured in the early log phase of growth on ethanol and the lowest in the early log phase of growth on glucose. however, the differences in the activities are much smaller than in saccharomyces cerevisiae and other yeasts under similar con ... | 1990 | 2170619 |
| chronic mastitis caused by candida maltosa in a cow. | 1990 | 2278138 | |
| occurrence of the general control of amino acid biosynthesis in yeasts. | the response of three amino acid biosynthetic enzymes, threonine dehydratase, tyrosine aminotransferase and saccharopine dehydrogenase, to conditions of histidine, tryptophan or lysine limitation was investigated in 15 yeast species. the activities of all these enzymes increased about two- to fourfold as a result of action of the general control of amino acid biosynthesis in brettanomyces anomalus, candida maltosa, hansenula polymorpha, rhodosporidium toruloides, saccharomyces cerevisiae and yar ... | 1990 | 2338623 |
| cyclic amp-dependent phosphorylation of fructose-1,6-bisphosphatase and other proteins in the yeast candida maltosa. | in crude extracts of candida maltosa, about 12 proteins are phosphorylated in the presence of camp or of a catalytic subunit of camp-dependent protein kinase. a strongly labelled protein spot occurred in the position of fructose-1,6-bisphosphatase both after electrophoresis of crude extracts incubated with camp and of a partially purified fructose-1,6-bisphosphatase incubated with a catalytic subunit of camp-dependent protein kinase. no phosphorylation of the cytoplasmic malate dehydrogenase cou ... | 1990 | 1965837 |
| regulation of chorismate mutase activity of various yeast species by aromatic amino acids. | the regulatory properties of chorismate mutase, its cellular localization and isoenzyme pattern were investigated in 23 yeast species. all yeasts contained only a single form of the enzyme, which is localized exclusively in the cytosol. the enzyme activity from all sources was activated 3-(rhodotorula aurantiaca) to 185-fold (candida maltosa) by tryptophan. the tryptophan concentration, which was necessary to obtain half maximum velocity was determined to be between 2 (pichia guilliermondii) and ... | 1991 | 2059012 |
| isolation and sequencing of a gene, c-ade1, and its use for a host-vector system in candida maltosa with two genetic markers. | the host-vector systems of an n-alkane-assimilating-yeast, candida maltosa, that we previously constructed consisted of a vector replicating with an ars region of this yeast, and c. maltosa strains j288 (leu2) or ch1 (his5) as hosts. since each of these hosts has a single genetic marker, we have developed a new host-vector system using two genetic markers. by uv irradiation of strain ch1, an adenine auxotrophic mutant, cha1, forming red colonies was isolated. a dna fragment complementing this de ... | 1991 | 1368674 |
| evidence that more than one gene encodes n-alkane-inducible cytochrome p-450s in candida maltosa, found by two-step gene disruption. | an n-alkane-assimilating yeast, candida maltosa, has a diploid genome. probed with the previously isolated gene of n-alkane-inducible cytochrome p-450 (p-450alk), its allelic gene had been isolated, its nucleotides sequenced, and the interallelic divergence examined. using one of the allelic genes, we disrupted the two alleles of the cytochrome p-450alk gene by a two-step gene disruption system. surprisingly, the disruptant still assimilated n-alkane and contained n-alkane-inducible cytochrome p ... | 1991 | 1368716 |
| degradation and dehalogenation of monochlorophenols by the phenol-assimilating yeast candida maltosa. | the phenol-assimilating yeast candida maltosa is able to degrade monochlorophenols but cannot grow on these substrates. 3- and 4-chlorophenol were broken down very rapidly by phenol-grown cells under the formation of 4-chlorocatechol, 5-chloropyrogallol and 4-carboxymethylenebut-2-en-4-olide with concomitant release of chloride. 2-chlorophenol was partially converted into cis,cis-2-chloromuconic acid via 3-chlorocatechol which was also obtained from 3-chlorophenol in low amounts. no further meta ... | 1991 | 1368963 |
| molecular cloning of the candida maltosa ade1 gene. | the structural gene (ade1) encoding phosphoribosyl-aminoimidazole-succinocarboxamide synthetase (saicar synthetase; ec 6.3.2.6) in candida maltosa has been isolated by functional complementation of an ade1 strain of saccharomyces cerevisiae. the gene was localized on a 2.5-kb bamhi dna fragment. nucleotide sequence analysis of the cloned gene has revealed an open reading frame encoding a protein (saicar synthetase) with an mr of 32,751. the codon bias index, 0.68, indicates that the ade1 gene is ... | 1991 | 1743515 |
| [biochemical basis of varying nystatin resistance of saccharomyces cerevisiae and candida maltosa mutants]. | six groups of nystatin resistant mutants of c. maltosa and of haploid and diploid saccharomyces cerevisiae strains were obtained with the help of genetic and biochemical analysis. it has been shown that every group of the mutants was characterized by a specific level of resistance to nystatin. the dependence of the resistance level upon sterol content has been established. it has been shown that the more the structure of the sterol present differed from ergosterol the higher was the resistance l ... | 1991 | 1819039 |
| sterol composition of nystatin-resistant candida maltosa mutants. | composition of sterol fractions of nystatin-resistant candida maltosa strains was determined. using uv-spectrometry, tlc and glc-ms it was demonstrated that resistance to nystatin is connected with the composition alterations of yeast cell sterols. block of different stages of ergosterol biosynthesis was revealed in some mutants, viz. c-24-transmethylation, delta 8----delta 7-isomerization, 14 alpha-demethylation, c-5(6)-dehydrogenation, reduction of c-14(15) and c-24(28) double bonds. | 1991 | 1823650 |
| correlation of biochemical blocks and genetic lesions in leucine auxotrophic strains of the imperfect yeast candida maltosa. | the four enzymatic steps in the conversion of alpha-ketoisovaleriate to leucine were examined in the wild type and in 13 leucine auxotrophic strains of candida maltosa. the genetic lesions in the auxotrophs, involve at least five different loci and are correlated with three enzymatic steps. this was confirmed by gene cloning, protoplast fusion, and enzyme assays. the pathway for leucine biosynthesis in c. maltosa shows general similarity to that of other lower eukaryotes but there are individual ... | 1991 | 1865875 |
| [isolation of antigen-active biopolymers from candida maltosa culture fluid for obtaining allergens of diagnostic value for examination of industrial microbiology workers]. | the membrane filtration technique and column gel chromatography+ (with sepharose 6b or sephacryl s-200) were used to receive fractions of kj candida maltosa, strain vsb 899, which were studied with highly effective liquid chromatography, immunochemical reactions and intracutaneous allergotesting on guinea-pigs sensibilized with vsb 899 live cultures. in the whole kj strain vsb 899 concentrate, 8 components were identified, six of which had molecular masses from 350,000 to 18,000 and had no analo ... | 1991 | 1879735 |
| comparison of two cytochromes p-450 from candida maltosa: primary structures, substrate specificities and effects of their expression in saccharomyces cerevisiae on the proliferation of the endoplasmic reticulum. | cdnas were cloned, sequenced and expressed which encode two different cytochrome p-450 forms of the alkane-assimilating yeast candida maltosa, designated as p-450cm1 and p-450cm2. the amino acid sequences deduced were about 55% identical. expression in saccharomyces cerevisiae resulted in the formation of intact microsomal p-450 systems catalyzing the hydroxylation of n-hexadecane and lauric acid with significantly different substrate preferences. a massive proliferation of the endoplasmic retic ... | 1991 | 1935996 |
| sequences of two tandem genes regulated by carbon sources, one being essential for n-alkane assimilation in candida maltosa. | several n-alkane-inducible clones were isolated from the genomic library of an n-alkane-assimilation yeast, candida maltosa, by the differential hybridization method. among these, one of the most predominantly expressed clones was analyzed. the nucleotide sequence of the cloned dna fragment showed that it contained two open reading frames, one encoding a protein of 127 amino acids (aa) and the other a protein of 276 aa. the former was named pox18cm, because the sequence was highly homologous to ... | 1991 | 1937042 |
| in situ localization of cytochrome p-450, the first enzyme involved in aliphatic hydrocarbon degradation in the yeast candida maltosa. | 1991 | 1947165 | |
| valine inhibition of beta-isopropylmalate dehydrogenase takes part in the regulation of leucine biosynthesis in candida maltosa. | the beta-isopropylmalate (ipm) dehydrogenase (ec 1.1.1.85) of candida maltosa, the third pathway-specific enzyme of leucine biosynthesis, was purified, some properties of the enzyme were studied and a novel regulatory pattern was found. the km values of the enzyme were estimated to be 0.42 mm for beta-ipm and 0.34 mm for nad+. it is demonstrated that the enzyme can be regulated by l-valine. the inhibition was competitive with respect to beta-ipm (ki = 1.84 mm) and non-competitive with respect to ... | 1991 | 1804028 |
| cyp52 (cytochrome p450alk) multigene family in candida maltosa: molecular cloning and nucleotide sequence of the two tandemly arranged genes. | southern blot analysis under low-stringency conditions using a previously isolated n-alkane-inducible cytochrome p450 (p450alk) gene as a probe revealed the presence of multiple p450alk-related genes in the genome of candida maltosa. nine p450alk-related genes (one reported previously and eight in the present report) were isolated from a genomic library constructed from this strain, and these were classified on the basis of sequence similarities into three pairs of putative allelic genes and thr ... | 1991 | 2039569 |
| steroid 11ß-hydroxylation by a fungal microsomal cytochrome p450. | the steroid 11ß-hydroxylase activity of the fungus cochliobolus lunatus was increased about 100-fold by cultivation of mycelia for 4-5 h with 20-hydroxymethyl-1,4-pregnadien-3-one. cell-free extracts revealed a maximum activity of 45 nmol 11ß-hydroxyprogesterone/h·mg protein in the 100,000 g pellet fraction. the 11ß-hydroxylation was dependent on nadph. the formation of 11ß-hydroxyprogesterone correlated linearly with the cytochrome p450 concentration. the fungal 11ß-hydroxylase transformed both ... | 1992 | 22217857 |
| efficient electropulse transformation of intact candida maltosa cells by different homologous vector plasmids. | conditions for efficient and quick transformation by electroporation were developed in candida maltosa. to investigate the efficiency of transformation with integrative as well as with autonomously replicating plasmids, a series of vectors was constructed for homologous transformation of this species. transformants were obtained with different plasmids as covalently closed circular molecules and as linearized dna. the influence of recipient strain and plasmid type as well as of cell number and p ... | 1992 | 1332307 |
| immunocytochemical localization of alkane-inducible cytochrome p-450 and its nadph-dependent reductase in the yeast candida maltosa. | antibodies directed against cytochrome p-450cm1 and the nadph-cytochrome p-450 reductase were used to study the induction and intracellular localization of these components of the alkane monooxygenase system in the yeast candida maltosa. transition from glucose to n-hexadecane utilization resulted in an about 100-fold increase of the immunodetectable p-450 form whereas the reductase was only moderately induced by a factor of about 5. p-450 but not the reductase was further increased by oxygen li ... | 1992 | 1511703 |
| molecular cloning and analysis of autonomous replicating sequence of candida maltosa. | a candida maltosa chromosomal dna fragment which confers high frequency transformation of c. maltosa and autonomous replication of recombinant plasmids was cloned and sequenced. analysis of the nucleotide sequence of the cloned dna revealed a sequence homologous for c. maltosa autonomously replicating sequence (ars) elements. vector prj1 for c. maltosa was constructed, which contained a 1.3 kb ars sequence, picem-19h and the ade1 gene of c. maltosa. southern blot analysis suggested that the copy ... | 1992 | 1514324 |
| cloning and sequence analysis of a candida maltosa gene which confers resistance to cycloheximide. | a cyhr gene from candida maltosa, which confers resistance to cycloheximide, was cloned in saccharomyces cerevisiae. a 2.3-kb dna fragment carrying this gene was sequenced, and an open reading frame able to encode 553 amino acids (aa) was found in the sequence. computer searches of the genbank, embl, swis-prot and gen-pept databases using the fasta program failed to detect any proteins with extensive similarities to the deduced aa sequence for cyhr. the cloned gene transforms s. cerevisiae at a ... | 1992 | 1628836 |
| drastic alteration of cycloheximide sensitivity by substitution of one amino acid in the l41 ribosomal protein of yeasts. | cycloheximide is one of the antibiotics that inhibit protein synthesis in most eukaryotic cells. we have found that a yeast, candida maltosa, is resistant to the drug because it possesses a cycloheximide-resistant ribosome, and we have isolated the gene responsible for this. in this study, we sequenced this gene and found that the gene encodes a protein homologous to the l41 ribosomal protein of saccharomyces cerevisiae, whose amino acid sequence has already been reported. two genes for l41 prot ... | 1992 | 1729213 |
| characterization of a novel enzyme, n6-acetyl-l-lysine: 2-oxoglutarate aminotransferase, which catalyses the second step of lysine catabolism in candida maltosa. | a novel aminotransferase catalyzing the second step of lysine catabolism, the oxidative transamination of the alpha-group of n6-acetyllysine, was identified and characterized in the yeast candida maltosa. the enzyme was strongly induced in cells grown on l-lysine as sole carbon source. its activity was specific for both n6-acetyllysine and 2-oxoglutarate. the km values were 14 mm for the donor, 4 mm for the acceptor and 1.7 microm for pyridoxal-5-phosphate. the enzyme had a maximum activity at p ... | 1992 | 1285645 |
| cloning and analysis of a candida maltosa gene which confers resistance to formaldehyde in saccharomyces cerevisiae. | a gene (fdh1) of candida maltosa which confers resistance to formaldehyde in saccharomyces cerevisiae was cloned and its nucleotide sequence determined. the gene has a single intron which possesses the highly conserved splicing signals found in s. cerevisiae introns. we demonstrated that processing of the pre-mrna of the cloned gene occurred identically in both s. cerevisiae and c. maltosa. the predicted amino acid sequence from the cloned gene showed 65.5% identity to human alcohol dehydrogenas ... | 1992 | 1339376 |
| evolutionary position of n-alkane-assimilating yeast candida maltosa shown by nucleotide sequence of small-subunit ribosomal rna gene. | we clarified the evolutionary position of candida maltosa, an n-alkane-assimilating yeast, by sequencing the nucleotides of the small-subunit ribosomal rna gene. phylogenetic analyses showed the close evolutionary relationships of c. maltosa with c. tropicalis, c. viswanathii, c. albicans, c. parapsilosis, and c. guilliermondii, forming a sub-group within this genus. | 1993 | 7764276 |
| overexpression of the er-membrane protein p-450 cyp52a3 mimics sec mutant characteristics in saccharomyces cerevisiae. | high expression of microsomal cytochrome p-450 cyp52a3 from candida maltosa induces the formation of membrane stacks in saccharomyces cerevisiae. membrane proliferation is accompanied by coinduction of the er proteins kar2p and sec61p and accumulation of precursor forms of proteins that have to translocate across the er membrane (kar2p, alpha factor). cytosolic proteins (ssa1p and 2p) and mitochondrial proteins (cyt c1p and f1 beta p) are not affected. n-terminal truncated p-450 proteins remain ... | 1993 | 8274497 |
| the alkane-inducible candida maltosa ali1 gene product is an nadh:ubiquinone oxidoreductase subunit homologue. | the ali1 gene product in candida maltosa was previously shown to be essential for n-alkane assimilation, possibly as a transcription factor [hwang et al., gene 106 (1991) 61-69]. we show that the predicted sequence is highly homologous to a subunit of respiratory complex i from another fungus, neurospora crassa, and from bos taurus. the predicted protein contains a motif conserved in this subunit from mitochondria, chloroplasts and bacteria. it also contains an n-terminal sequence that suggests ... | 1993 | 8299970 |
| cloning of the c-ura3 gene and construction of a triple auxotroph (his5, ade1, ura3) as a useful host for the genetic engineering of candida maltosa. | the c-ura3 gene of the n-alkane assimilating-yeast candida maltosa was cloned by complementation of the ura3 mutation of saccharomyces cerevisiae. the nucleotide sequence of c-ura3 and its deduced amino-acid sequence showed significant homology to those of the orotidine 5'-phosphate decarboxylases of other fungal species. to construct a useful host for genetic engineering of c. maltosa using c-ura3 as a marker, one allele of c-ura3 in a double auxotroph (his5, ade1) was disrupted by c-ade1, and ... | 1993 | 8435849 |
| primary structure of the ade1 gene from candida utilis. | the ade1 gene from candida utilis ca(u)-37, a strain used for commercially producing enzymes, was cloned by complementation of the ade1 mutation of saccharomyces cerevisiae. it was composed of 903 bp, and the deduced amino acid sequence was 70% homologous to those of the ade1 genes of s. cerevisiae and candida maltosa. the highly preserved region of saicar synthetase, the ade1 gene product, was also found by a homology search. | 1994 | 7764516 |
| molecular analysis of a leu2-mutant of candida maltosa demonstrates the presence of multiple alleles. | three different alleles of the beta-isopropylmalate dehydrogenase gene were cloned and sequenced from a leucine auxotrophic mutant, g587, of candida maltosa. the cloning of functionally-intact wild-type genes from this mutant strain suggests the presence of silent gene copies. an interallelic-divergence comparison has provided evidence for new regulatory mechanisms. sequence data and karyotype analysis argue for a highly-aneuploid genome of c. maltosa. an interpretation for the spontaneous auxot ... | 1994 | 7859302 |
| purification and some properties of alanine aminotransferase from candida maltosa. | alanine aminotransferase (alaat; ec 2.6.1.2) was purified to homogeneity from candida maltosa that was grown on l-alanine as the sole source of carbon and nitrogen. the enzyme has a molecular mass of 99kda and consists of two subunits of equal molecular mass (52 kda). each subunit binds one mole of plp. the enzyme has an isoelectric point of 5.3 and an optimum ph of 6.0-7.5. the spectroscopic profile and an inhibition experiment showed that both plp and free-sh groups are directly involved in th ... | 1994 | 7764540 |
| candida albicans gene encoding resistance to benomyl and methotrexate is a multidrug resistance gene. | candida albicans is not inhibited by a number of drugs known to affect fungal cells. the basis for this resistance in most cases is unknown but has been attributed to the general impermeability of the fungal cell envelope. a gene (benr) formerly shown to be responsible for the resistance of c. albicans to benomyl and methotrexate was shown in the present study to confer resistance to four other inhibitory compounds: cycloheximide, benztriazoles, 4-nitroquinoline-n-oxide, and sulfometuron methyl. ... | 1994 | 8031026 |
| highly-efficient electrotransformation of the yeast hansenula polymorpha. | a highly-efficient method for transformation of the methylotrophic yeast hansenula polymorpha has been developed. routinely, transformation frequencies of up to 1.7 x 10(6)/micrograms plasmid dna were obtained by applying an electric pulse of the exponential decay type of 7.5 kv/cm to a highly-concentrated cell mixture during 5 ms. efficient transformation was dependent on: (1) pretreatment of the cells with the reducing agent dithiotreitol, (2) the use of sucrose as an osmotic stabilizer in an ... | 1994 | 8082173 |
| expression of an endogenous and a heterologous gene in candida maltosa by using a promoter of a newly-isolated phosphoglycerate kinase (pgk) gene. | a gene encoding phosphoglycerate kinase (pgk) was isolated from the genomic library of c. maltosa to construct an expression vector for this yeast. the pgk gene had an open reading frame of 1,251 base pairs encoding approximately 47-kda polypeptide of 417 amino-acid residues. expression of this gene assayed by northern-blot analysis was significantly induced in cells grown on glucose but not in cells grown on n-tetradecane, n-tetradecanol, or oleic acid. by using the promoter region of this gene ... | 1994 | 8082186 |
| transformation of candida maltosa by electroporation. | 1995 | 7550747 | |
| biodegradation of nonionic surfactants. i. biotransformation of 4-(1-nonyl)phenol by a candida maltosa isolate. | results are reported concerning biodegradation of 4-(1-nonyl)phenol by cultures of a candida maltosa strain isolated from aerobic sludge samples collected at a depuration plant treating wastewaters from a textile industry. the yeast was able to utilize 4-(1-nonyl)phenol as a sole carbon and energy source. preliminary attempts to draw the actual metabolic pathway evidenced microbial attack on the alkyl chain with the production of 4-acetylphenol. to the best of our knowledge this is the first rep ... | 1995 | 15091504 |
| proliferation of intracellular membrane structures upon homologous overproduction of cytochrome p-450 in candida maltosa. | in an alkane-assimilating yeast, candida maltosa, a cultivation on alkane causes both induction of endoplasmic reticulum (er)-resident membrane proteins, such as cytochrome p-450, and proliferation of er. in this study, individual genes for alkane-inducible forms of cytochrome p-450 (p-450alk) were homologously overexpressed in c. maltosa using a galactose-inducible expression system developed in this yeast. immunoelectron microscopy revealed that, upon the overexpression, a dramatic proliferati ... | 1995 | 7794946 |
| cyp52 (cytochrome p450alk) multigene family in candida maltosa: identification and characterization of eight members. | previously, we characterized three genes and presented evidence for an n-alkane-inducible cytochrome p450 (p450alk) multigene family in an n-alkane-assimilating and diploid-type yeast, candida maltosa. in the present report, we isolated and characterized additional members of this gene family, including a total of thirteen p450alk-related sequences (eight genes and five of their alleles). two sets, each consisting of two genes, were tandemly arranged in the genome. a gene replacement experiment ... | 1995 | 7865134 |
| evidence that the expression of the gene for nadph-cytochrome p-450 reductase is n-alkane-inducible in candida maltosa. | a gene coding for nadph-cytochrome p-450 reductase of an n-alkane-assimilating yeast, candida maltosa, was isolated and sequenced. northern analysis and assay of the expression of the reporter gene under the control of the promoter of this gene showed that the transcriptional level was induced 4 to 8-fold in cells grown on n-alkane relative to cells grown on glucose. | 1995 | 7545482 |
| chromosome polymorphisms close to the cm-ade1 locus of candida maltosa. | the imperfect yeast candida maltosa has an ill-defined genetic constitution; it is nominally diploid, but probably highly aneuploid, in nature. we report on polymorphisms specifically affecting those chromosomes which bear the cm-ade1 gene. this gene encodes phosphoribosylaminoimidazole-succino-carboxamide synthetase, an enzyme in the adenine biosynthetic pathway. by electrophoretic karyotype analysis, three differently sized chromosomes were demonstrated to carry cm-ade1; the size (but not the ... | 1995 | 7603439 |
| in vivo reconstitution of highly active candida maltosa cytochrome p450 monooxygenase systems in inducible membranes of saccharomyces cerevisiae. | to establish a system for functional characterization of individual candida maltosa cytochrome p450 monooxygenases, the nadph-cytochrome p450 reductase from this yeast species was co-expressed in saccharomyces cerevisiae with each of the following cytochrome p450 forms; p450cm1 (cyp52 a3), p450cm2 (cyp52 a4), and p450alk2a (cyp52 a5). for this purpose, a multicopy plasmid was constructed that contained two independent expression units controlled by the galactose-inducible gal10 promoter. as show ... | 1995 | 7626221 |
| inducible expression of a gene encoding an l41 ribosomal protein responsible for the cycloheximide-resistant phenotype in the yeast candida maltosa. | in a previous paper (s. kawai, s. murao, m. mochizuki, i. shibuya, k. yano, and m. takagi, j. bacteriol. 174:254-262, 1992), we showed that in each genome of several yeast species, there is one of two types of l41 gene, one for an l41 (q-type) protein which confers cycloheximide (cyh) resistance or one for an l41 (p-type) protein which does not. these genes have been suggested to be responsible for the cyh response used in taxonomy. for example, saccharomyces cerevisiae, which is cyh sensitive, ... | 1995 | 7665534 |
| a deviation from the universal genetic code in candida maltosa and consequences for heterologous expression of cytochromes p450 52a4 and 52a5 in saccharomyces cerevisiae. | we demonstrate that serine instead of leucine is specified by the cug codon in the yeast candida maltosa. evidence for this deviation from the universal genetic code was obtained by means of in vitro translation experiments. depending on the cell-free system used, either serine, in the c. maltosa system, or leucine, in the control with the conventional wheat germ system, was found to be incorporated into the translation products of artificial cug-containing mrnas. moreover, we were able to trans ... | 1995 | 7762299 |
| in vivo evidence for non-universal usage of the codon cug in candida maltosa. | an alkane-assimilating yeast candida maltosa had been studied in order to establish systems suitable for biotransformation of hydrophobic compounds. however, functional expression of heterologous genes tested for this purpose had not been successful in several cases. on the other hand, it had been reported that the codon cug, a universal leucine codon, is read as serine in c. cylindracea. the same altered codon usage had also been suggested by in vitro experiments in some candida yeasts which ar ... | 1995 | 7762300 |
| the sequence of a 44 420 bp fragment located on the left arm of chromosome xiv from saccharomyces cerevisiae. | we have determined the complete nucleotide sequence of a 44 420 bp dna fragment from chromosome xiv of saccharomyces cerevisiae. the sequence data revealed 23 open reading frames (orfs) larger than 300 bp, covering 73.5% of the sequence. the orfs n2418, n2428, n2441, n2474 and n2480 correspond to previously sequenced s. cerevisiae genes coding respectively for the mitochondrial import protein mas5, the nucleolar protein nop2, the outer mitochondrial membrane porin por1, the cytochrome c oxidase ... | 1995 | 8533472 |
| identification of a centromeric activity in the autonomously replicating tra region allows improvement of the host-vector system for candida maltosa. | a centromeric activity was identified in the previously isolated 3.8 kb dna fragment that carries an autonomously replicating sequence (ars) from the yeast candida maltosa. plasmids bearing duplicated copies of the centromeric dna (dicentric plasmids) were physically unstable and structural rearrangements of the dicentric plasmids occurred frequently in the transformed cells. the centromeric dna activity was dissociated from the ars, which is 0.2 kb in size, and was delimited to a fragment at le ... | 1995 | 8552050 |
| cloning and characterization of the pox2 gene in candida maltosa. | to study the function of acyl-coa oxidase in an n-alkane-assimilating yeast, candida maltosa, we isolated the pox2 gene which is a member of the acyl-coa oxidase gene family. pox2 had a 2172-bp open reading frame (orf) encoding an approx. 84-kda polypeptide (724 amino acids (aa)) and was contiguous to pox4, another member of the acyl-coa oxidase gene family on the same chromosomal dna in a convergent arrangement. northern blot analysis revealed that the expression of pox2 was induced in cells gr ... | 1995 | 8566769 |
| the distribution of nd genes in yeast mitochondrial genomes and the mitochondrial dna structure of pichia membranaefacens. | for long time, it has been believed that the yeast mitochondrial (mt) genome lacks nadh dehydrogenase subunit genes which are designated nd genes. however, our complete mtdna sequencing of yeast hansenula wingei led us to the first finding of seven mitochondrial nd genes. we investigated the distribution of nd genes in mtdnas of other yeasts including pichia membranaefaciens, yarrowia lypolitica, candida maltosa, saccharomyces kluyveri and saccharomyces exiguus. by southern hybridization with pr ... | 1995 | 8841533 |
| characterization of the n-alkane and fatty acid hydroxylating cytochrome p450 forms 52a3 and 52a4. | two enzymes, p450 52a3 (p450cm1) and 52a4 (p450cm2), the genes of which belong to the cyp52 multigene family occurring in the alkane-assimilating yeast candida maltosa, have been characterized biochemically and compared in terms of their substrate specificities. for this purpose, both the p450 proteins and the corresponding c. maltosa nadph-cytochrome p450 reductase were separately produced by expressing their cdnas in saccharomyces cerevisiae, purified, and reconstituted to active monooxygenase ... | 1996 | 8645001 |
| peroxisome proliferators activate cytochrome p450 genes in an alkane-assimilating yeast, candida maltosa. | candida maltosa can assimilate n-alkane as a sole carbon source and cytochromes p450alk (p450alk) are critical for the first oxidation step. four major p450alks that are encoded by genes alk1, alk2, alk3 and alk5 are induced by n-alkane and repressed by glucose at the transcriptional level. in the present work, we found that all these four genes but alk5 are transcriptionally activated in response to a peroxisome proliferator, clofibrate. this is the first report on the peroxisome proliferator r ... | 1996 | 8651924 |
| initial oxidative and subsequent conjugative metabolites produced during the metabolism of phenanthrene by fungi. | three filamentous fungi were examined for the ability to biotransform phenanthrene to oxidative (phase i) and conjugative (phase ii) metabolites. phenanthrene metabolites were purified by high-performance liquid chromatography (hplc) and identified by uv/visible absorption, mass, and 1h nmr spectra. aspergillus niger atcc 6275, syncephalastrum racemosum ut-70, and cunninghamella elegans atcc 9245 initially transformed [9-(14)c]phenanthrene to produce metabolites at the 9,10-, 1,2-, and 3,4-posit ... | 1996 | 8652115 |
| candida maltosa nadph-cytochrome p450 reductase: cloning of a full-length cdna, heterologous expression in saccharomyces cerevisiae and function of the n-terminal region for membrane anchoring and proliferation of the endoplasmic reticulum. | a full-length cdna for nadph-cytochrome p450 reductase from candida maltosa was cloned and sequenced. the derived amino acid sequence showed a high similarity to the reductases from other eukaryotes. expression in saccharomyces cerevisiae under control of the gal10 promoter resulted in an approximately 70-fold increase in nadph-cytochrome c reductase activity in the microsomal fraction. the functional integrity of the heterologously expressed reductase as an electron transfer component for alkan ... | 1996 | 8701606 |
| the cyp52 multigene family of candida maltosa encodes functionally diverse n-alkane-inducible cytochromes p450. | the n-alkane-assimilating yeast candida maltosa contains several structurally related cytochromes p450 (p450) encoded by the cyp52 multigene family, which are inducible by various long-chain hydrocarbons and fatty acids and which are responsible for the initial hydroxylation steps in the metabolism of these substrates. in the present work, the four major n-alkane-inducible c. maltosa p450 forms; cyp52a3, cyp52a4, cyp52a5, and cyp52a9, were enzymatically characterized, taking advantage of heterol ... | 1996 | 8713123 |
| enhanced excretion of intermediates of aromatic amino acid catabolism during chlorophenol degradation due to nutrient limitation in the yeast candida maltosa. | incubation of phenol-induced cells of the yeast candida maltosa sbug 700 with mono- and dichlorophenols resulted in the formation of metabolites of the substrates and of further metabolites not related to the degradation pathway of the substrates. these additional compounds, identified as 4-hydroxyphenylacetic acid (4-hpa), phenylacetic acid (pa), indolylacetic acid (ia) and indolylethanol (i.e.) by means of hplc and gc/ms, were not excreted in incubation experiments with glucose. the excretion ... | 1996 | 8765083 |
| 3-phosphoglycerate kinase: a glycolytic enzyme protein present in the cell wall of candida albicans. | we have used a polyclonal antiserum to cell wall proteins of candida albicans to isolate several clones from a cdna lambda gt11 expression library. affinity-purified antibody prepared to the fusion protein of one clone identified a 40 kda moiety present in cell wall extracts from both morphologies of the organism. indirect immunofluorescence demonstrated expression of this moiety at the c. albicans cell surface. sequencing of a pbluescript ii genomic clone identified with the cdna clone revealed ... | 1997 | 9043109 |
| galactose-inducible expression systems in candida maltosa using promoters of newly-isolated gal1 and gal10 genes. | the gal1 and gal10 gene cluster encoding the enzymes of galactose utilization was isolated from an asporogenic yeast, candida maltosa. the structure of the gene cluster in which both genes were divergently transcribed from the central promoter region resembled those of some other yeasts. the expression of both genes was strongly induced by galactose and repressed by glucose in the medium. galactose-inducible expression vectors in c. maltosa were constructed on low- and high-copy number plasmids ... | 1997 | 9046083 |
| candida aquaetextoris sp. nov., a new species of yeast occurring in sludge from a textile industry wastewater treatment plant in tuscany, italy. | we describe candida aquaetextoris, a new yeast species isolated from sludge that accumulates at the main wastewater treatment facility which processes discharges from textile factories located in the prato metropolitan district, northern tuscany, italy. this yeast degrades 4-(1-nonyl)phenol, a toxic intermediate originating from the microbial attack of nonylphenol polyethoxylates, which are nonionic surfactants largely used in leather and textile industries. in the investigation we employed conv ... | 1997 | 9103618 |
| transformation of chlororesorcinol by the hydrocarbonoclastic yeasts candida maltosa, candida tropicalis, and trichosporon oivide. | the inhibitory effects of chlorinated monoaromatic compounds on three hydrocarbonoclastic yeasts grown on glucose or resorcinol were examined. at concentrations of 1.0 m, all of the monoaromatic compounds were inhibitory. when the concentration of chlororesorcinol was significantly reduced (0.0005 m), the inhibition to each yeast was minimized. extracts of the cultures of yeasts growing on resorcinol plus chlororesorcinol were analyzed for residual resorcinol and chlororesorcinol with high press ... | 1997 | 9236299 |
| evidence that part of a centromeric dna region induces pseudohyphal growth in a dimorphic yeast, candida maltosa. | we observed that a ycp-type vector having the centromeric dna (cen) sequence previously isolated from the genome, but not a yrp-type vector lacking the cen sequence, induced pseudohyphal growth in a dimorphic fungi, candida maltosa, which had been shown to be closely related to candida albicans by phylogenetic analysis. deletion analysis of the cen sequence revealed that the intact cen sequence was not required for the induction, but part of it, having partial centromeric activity, was enough fo ... | 1997 | 9260943 |
| oxidation of exogenous formaldehyde in methylotrophic and nonmethylotrophic yeast cells. | nonmethylotrophic (candida maltosa and saccharomyces cerevisiae) and methylotrophic (hansenula polymorpha) yeast cells acidified their incubation media in the presence of formaldehyde. this was associated with the release of formate. we studied the formaldehyde-dependent production of formic acid and the enzymatic properties of these strains grown on media containing various carbon sources. the acidifying potential was considerably lower in formaldehyde dehydrogenase-deficient cells of mutant st ... | 1997 | 9284545 |
| seasonal occurrence of yeasts and yeast-like organisms in the river danube. | one hundred and seventy yeast strains belonging to 14 genera and 29 species were isolated from 112 water samples of the river danube in the area of bratislava. the samples were collected through the year from april to march. saccharomyces cerevisiae, candida maltosa, aureobasidium pullulans, cystofilobasidium capitatum, rhodotorula glutinis, geotrichum candidum, and candida krusei were the most frequent. the basidiomycetous yeasts and yeast-like organisms with oxidative metabolism were present i ... | 1997 | 9298185 |
| isozyme function of n-alkane-inducible cytochromes p450 in candida maltosa revealed by sequential gene disruption. | an n-alkane-assimilating yeast candida maltosa contains multiple n-alkane-inducible forms of cytochromes p450 (p450alk), which can be assumed to catalyze terminal hydroxylation of n-alkanes in the assimilation pathway. eight structurally related p450alk genes have been identified. in the present study, the function of four major isoforms of p450alk (encoded by alk1, alk2, alk3, and alk5 genes) was investigated by sequential gene disruption. auxotrophic markers used for the selection of disrupted ... | 1998 | 9461581 |
| the spl1 trna splicing gene of candida maltosa and candida albicans. | 1998 | 9544248 | |
| isolation and characterization of epd1, an essential gene for pseudohyphal growth of a dimorphic yeast, candida maltosa. | additional copies of the centromeric dna (cen) region induce pseudohyphal growth in a dimorphic yeast, candida maltosa (t. nakazawa, t. motoyama, h. horiuchi, a. ohta, and m. takagi, j. bacteriol. 179:5030-5036, 1997). to understand the mechanism of this transition, we screened the gene library of c. maltosa for sequences which could suppress this morphological change. as a result, we isolated the 5' end of a new gene, epd1 (for essential for pseudohyphal development), and then cloned the entire ... | 1998 | 9555889 |
| a strong nitrogen source-regulated promoter for controlled expression of foreign genes in the yeast pichia pastoris. | in methylotrophic yeasts, glutathione-dependent formaldehyde dehydrogenase (fld) is a key enzyme required for the metabolism of methanol as a carbon source and certain alkylated amines such as methylamine as nitrogen sources. we describe the isolation and characterization of the fld1 gene from the yeast pichia pastoris. the gene contains a single short intron with typical yeast-splicing signals near its 5' end, the first intron to be demonstrated in this yeast. the predicted fld1 product (fld1p) ... | 1998 | 9714758 |
| mutual conversion of fatty-acid substrate specificity by a single amino-acid exchange at position 527 in p-450cm2 and p-450alk3a. | the two eukaryotic fatty-acid hydroxylases p-450cm2 and p-450alk3a, which represent cyp52a4 variants naturally occurring in the yeast candida maltosa, were characterized with respect to their substrate specificity. whereas p-450cm2 was found to catalyse lauric acid omega-hydroxylation with greater efficiency, p-450alk3a had higher palmitic acid turnover numbers compared to p-450cm2, resulting in ratios of lauric acid to palmitic acid turnover rates of nearly 11 and 3 for p-450cm2 and p-450alk3a, ... | 1998 | 9760180 |