Publications
Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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the wax constituents of forage grasses: cocksfoot and perennial ryegrass. | 1931 | 16744781 | |
the glyceride fatty acids of forage grasses: cocksfoot and perennial ryegrass. | 1932 | 16744806 | |
observations on the vertical migrations of the third-stage larva of haemonchus contortus (rud.) on experimental plots of lolium perenne s24, in relation to meteorological and micrometeorological factors. | 1950 | 15401178 | |
factors influencing agnotobiotic cultures of the rumen ciliate, entodinium simplex. | the nutrition of entodinium simplex was studied, with foliage of bluegrass (poa pratense), perennial ryegrass (lolium perenne), and grains of wheat (triticum vulgare) as substrates in agnotobiotic cultures. entodinium grew poorly when the substrates were autoclaved; better growth was obtained when the substrates were sterilized with ethylene oxide vapor. the concentration of ethylene oxide and the amount of moisture influenced the sterility and nutritional adequacy of the treated substrate. auto ... | 1968 | 4969666 |
the urinary aromatic acids excreted by sheep given s24 perennial ryegrass cut at six stages of maturity. | 1970 | 5484732 | |
in vivo digestibility of cell-wall polysaccharides of setaria splendida and lolium perenne dv. kangaroo valley. | 1973 | 4764244 | |
application of in vitro and in vivo assay techniques in the isolation of rye grass (lolium perenne) pollen allergens. | 1974 | 4214790 | |
the nutritive value of dried red clover as a supplement for perennial ryegrass hay. | 1974 | 4422645 | |
the digestion of fresh, frozen and dried perennial ryegrass. | 1974 | 4459975 | |
rast, skin and nasal tests and the history in grass pollen allergy. | a comparison of the rast (phadebas), skin prick test, nasal test and a history of hay fever was made in 141 subjects, eighty-seven being prick test positive, with three concentrations of extracts of sweet vernal (anthoxanthum odoratum), cocksfoot (dactylis glomerata), meadow fescue (festuca elatior), rye (lolium perenne), timothy (phleum pratense), meadow (poa pratensis). in adequate concentration any of the species extracts would have sufficed for skin tests, with cocksfoot pollen the most pote ... | 1975 | 1053442 |
studies on the flow of zinc, cobalt, copper and manganese along the digestive tract of sheep given fresh perennial ryegrass, or white or red clover. | 1. sheep fitted with a rumen fistula and either a re-entrant cannula at the proximal duodenum or a re-entrant cannula at the terminal ileum were given twice daily 480-520 g dry matter as fresh perennial ryegrass, or white or red clover. flows of digesta were corrected to 100% recovery of chronic oxide. 2. the quantities (g/24 h) of zince and cobalt leaving the stomach were significantly greater than those in the food. no significant change was found in the quantities of copper and manganese. sig ... | 1975 | 1148155 |
numerical taxonomy of some yellow-pigmented bacteria isolated from plants. | phenetic data on over 60 heterotrophic, gram-negative, yellow chromogenic bacteria from plant material were collected and analysed using numerical taxonomic methods. marker strains representing 42 taxa were included in the analyses. at similarity levels of 80% or above, eight distinct clusters were obtained, the first four of which included yellow chromogens. custer i contained isolates from green healthy leaves of agrostis tenuis, festuca rubra, holcus lanata, lolium perenne and poa pratensis, ... | 1976 | 1011010 |
the effect of light and temperature on the growth of two subtropical and two temperate grass species. | the effect of light intensity and temperature on the growth of young plants of two temperate (lolium perenne, festuca arundinacea) and two subtropical (lolium rigidum, bromus unioloides) grass species was studied in the northern part of the gdr under open-air conditions. it was found that the subtropical species reacted highly sensitive to changes in the two environmental factors and showed absolutely higher growth performance during the summer as compared with the temperate species. the multipl ... | 1976 | 985302 |
the rumen flagellate piromonas communis: its life-history and invasion of plant material in the rumen. | the rumen flagellat piromonas communis is the zoospore of a phycomycete fungus inhabiting the rumen. zoosporogenesis was stimulated by a dietary component (the inducer), and inhibited by compounds affecting membrane structure and function, but not by inhibitors of protein synthesis. the zoospores showed taxis towards the tissues surrounding the inflorescence of lolium perenne l. in the rumen, invading principally the stomata and damaged tissues. the zoospores germinated on this substratum and th ... | 1977 | 16983 |
on the induction of zoosporogenesis in the rumen phycomycetes neocallimastix frontalis, piromonas communis and sphaeromonas communis. | the plant components inducing zoosporogenesis in the rumen phycomycetes neocallimastix frontalis, sphaeromonas communis and piromonas communis were widely distributed in the plant kingdom with no apparent taxonomic relationship. in lolium perenne l. (perennial rye-grass) and hordeum distichon (barley), the components were principally present in the leaves and aerial tissues. sufficient inducer was present in the normal diet of the host animal to trigger the differentiation and release of the zoo ... | 1977 | 21939 |
antagonistic interactions of phylloplane bacteria with drechslera dictyoides (drechslera) shoemaker. | strains of listeria denitrificans (e2), pseudomonas fluorescens (c37 and c92), and xanthomonas campestris (d119), isolated from the phylloplane of lolium perenne (s24), were antagonistic to drechslera dictyoides (drechslera) shoemaker. from in vitro and in vivo experiments it was deduced that their mode of activity included an initial inhibition of spore germination, a retardation in the rate of germ-tube elongation, and ultimately lysis of the hyphae. the effects were expressed on the plant in ... | 1977 | 406025 |
genetic control of resistance to crown rust (puccinia coronato corda) in lolium perenne l. and its implications in breeding. | a genetic analysis of resistance to crown rust in a series of natural populations of lolium perenne by means of the diallel cross has revealed that heritability is high (58%) and that the genes concerned show complete but ambi-directional dominance for resistance. an environmental correlation was established between the degree of infection and productivity but no genetic correlation was present. the implications of this mode of control for resistance breeding procedures are briefly considered. | 1977 | 24317594 |
the nutritive value of silages. energy metabolism in sheep receiving diets of grass silage or grass silage and barley. | 1. two calorimetric experiments were conducted to study the utilization of energy in sheep given diets of grass silage or grass silage and barley. three silages were investigated. one was made from first-harvest grass in the spring (s) and the others from regrowth cut either early or late in the autumn (e and l respectively). all were of perennial ryegress (lolium perenne) and preserved with formic acid. each silage was given at two levels of feeding, the lower providing approximately a maintena ... | 1978 | 698160 |
adhesion of bacteroides succinogenes in pure culture and in the presence of ruminococcus flavefaciens to cell walls in leaves of perennial ryegrass (lolium perenne). | bacteroides succinogenes and ruminococcus flavefaciens are two of the most important cellulolytic bacteria in the rumen. adhesion of b. succinogenes in pure culture, and in mixed culture with r. flavefaciens, to the various types of cell walls in sections of perennial ryegrass (lolium perenne l. cultivar s24) leaves was examined by transmission and scanning electron microscopy. b. succinogenes adhered to the cut edges of most plant cell walls except those of the meta- and protoxylem. it also adh ... | 1978 | 567035 |
ruminococcus flavefaciens cell coat and adhesion to cotton cellulose and to cell walls in leaves of perennial ryegrass (lolium perenne). | ruminococcus flavefaciens was shown to possess a prominent glycoprotein coat, which contained rhamnose, glucose, and galactose as its principal carbohydrates. periodate-reactive carbohydrate occurred as a surface layer of the coat. the ruminococci adhered strongly by means of this coat to cotton cellulose and to cell walls in leaf sections of lolium perenne l. (perennial ryegrass). the coat was diffuse at the point of contact so that the bacterial cell wall was in close contact with the substrat ... | 1978 | 16345261 |
the effect of formaldehyde treatment before ensiling on the digestion of wilted grass silage by sheep. | 1. wilted perennial ryegrass (lolium perenne l. cv. endura) was ensiled without additive or after addition of a mixture of equal volumes of formic acid (850 g/kg) and formalin (380 g formaldehyde/kg) applied at a rate of 35 g formaldehyde/kg herbage crude protein (nitrogen x 6.25). the digestion of the two silages and the effect of supplemental n as urea or urea plus soya-bean meal on the digestion of the treated silage was studied using sheep fitted with a rumen cannula and re-entrant cannulas ... | 1979 | 574400 |
some factors associated with the difference in nutritive value of artificially dried red clover and perennial ryegrass for sheep. | 1979 | 486389 | |
polyploidy and domestication: the origin and survival of polyploids in cytotype mixtures. | the origin and survival of a polyploid in a mixture of this polyploid and its parent(s) is reviewed. with several examples a picture is drawn of the interference of cytotypes in a mixture of cytotypes. some natural polyploids, both wild and domesticated, are very successful. they, like bread wheat and banana, largely replaced their parents. the same is true for some artificial polyploids like autotriploid hybride sugar beet in europe and autotetraploid perennial ryegrass. but when grown together ... | 1979 | 550834 |
the nutritive value of silages. digestion of organic matter, gross energy and carbohydrate constituents in the rumen and intestines of sheep receiving diets of grass silage or grass silage and barley. | 1. two experiments were conducted to study the digestion of organic matter, gross energy and carbohydrate constituents in the rumen, small intestine and caecum and colon of sheep given grass silage diets. three silages made from perennial ryegrass (lolium perenne) with formic acid as an additive were used. one was made from first-harvest grass in the spring and the others from regrowth grass cut from a single sward in either early autumn or late autumn. expt 1 involved a comparison between the s ... | 1980 | 6251860 |
inhibition of murine reaginic antibody responses by nasal immunotherapy with modified allergen. | optimal conditions were established for induction of reaginic antibodies to lolium perenne pollen allergens in mice by intranasal dosing of allergens with bordetella pertussis vaccine. this antibody response could be inhibited by pretreatment of the mice by nasal administration of 100 microgram of glutaraldehyde-modified l. perenne allergens 9 times in 3 weeks before priming, whereas native allergens, in doses of 5 microgram, did not inhibit an ige response to subsequent priming. it was not poss ... | 1980 | 6769824 |
the nutritive value of silages. digestion of nitrogenous constituents in sheep receiving diets of grass silage and grass silage and barley. | 1. two experiments were conducted to study the digestion of nitrogenous constituents in the rumen, small intestine and caecum and colon of sheep given diets of grass silage or grass silage and barley. three silages were used. one was made from first-harvest grass in the spring and the other from regrowth grass cut in either early autumn or late autumn. all were of perennial ryegrass (lolium perenne) and were preserved with formic acid. 2. expt 1 involved a comparison between the spring silage g ... | 1980 | 7417392 |
standardization of allergen extracts by inhibition of rast, skin test, and chemical composition. | five allergen extracts of dermatophagoides pteronyssinus, lolium perenne, alternaria tenuis, aspergillus fumigatus and cladosporium herbarum, obtained from four different manufacturers, were examined by inhibition of rast, content of protein and carbohydrate, contents of phosphorylcholine (pc) and tridacnin reactive components, and by skin test. inhibition of rast was used as a primary method for establishing allergenic potency and demonstrated wide variations for each preparation supplied by th ... | 1980 | 7004662 |
effects of temperature on rate of feeding of the plant parasitic nematodes rotylenchus robustus, xiphinema diversicaudatum, and hemicycliophora conida. | rotylenchus robustus, xiphinema diversicaudatum, and hemicycgiophora conida were observed feeding over a range of temperatures on perennial rye-grass (lolium perenne) seedlings grown on agar plates. r. robustus fed between 0.5 and 42.5 c, x. diversicaudatum between 5.0 and 37.0 c and h. conida between 5.0 and 34.0 c. between 10 and 25 c there was a direct relationship between temperature and rate of esophageal bulb contractions. above 25 c the number of esophageal contractions/min did not increa ... | 1980 | 19300695 |
isotopic fractionation associated with symbiotic n(2) fixation and uptake of no(3) by plants. | isotopic fractionation associated with n(2) fixation and no(3) (-) uptake by plants are relevant to the accuracy of estimates of n(2) fixation based on differences in the natural abundance of (15)n between n(2) fixing and nonfixing plants. the isotope effect on n(2) fixation by soybeans (glycine max [l] merrill, variety harosoy) and red clover (trifolium pratense [l]) was determined from the difference in (15)n abundance between atmospheric n(2) and the total n of plants grown hydroponically wit ... | 1980 | 16661392 |
description of trilineellus clathrocutis n.g., n.sp. (tylenchorhynchinae: tylenchida thorne, 1949) with a key to species and observations on tylenchorhynchus sensu stricto. | trilineeilus clathrocutis n.g., n.sp. is described and illustrated. it was found as an associate of corn (zea mays) in stockton, georgia, usa, and is related to a group of tylenchorhynchus sensu lato species having three lines in nonareolated lateral fields. this new species is closely related to tylenehorhynehus divittatus siddiqi 1961, t. sculptus seinhorst 1963, and t. triglyphus seinhorst 1963 (syn. t. chonai sethi & swarup 1968) tarjan 1973. it differs from these species primarily by having ... | 1981 | 19300734 |
grass pollen hyposensitization versus placebo therapy. i. clinical effectiveness and methodological aspects of a pre-seasonal course of desensitization with a four-grass pollen extract. | the present work reports the results of a double-blind clinical trial, comparing the effects of hyposensitization treatment versus placebo in 33 patients with allergic rhinitis, sensitive to a crude extract of the pollen of four different grasses (dactylis glomerata, lolium perenne, secale cereale, and phleum pratense). the distribution of these patients in the two groups was done randomly and gave two comparable groups, as far as clinical and biological features are concerned. the treatment cou ... | 1981 | 7032341 |
the digestion by cattle of grass silage made with formic acid and formic acid-formaldehyde. | 1. a primary growth crop of perennial ryegrass (lolium perenne l., cv. s23) was partially wilted and ensiled after the application of either formic acid-water (1:3, w/v; 7.11 l/t fresh herbage; control diet c) or formic acid-formaldehyde (1:1, w/w; 8.8 l/t fresh herbage; formaldehyde treated diet f) which supplied 50 g hcho/kg crude protein (nitrogen (n) x 6.25). the two silages were fed separately and a third diet comprising formaldehyde-treated silage, supplemented with urea (20 g/kg dry matte ... | 1981 | 7260029 |
protein metabolism in growing lambs fed on fresh ryegrass (lolium perenne) -clover (trifolium repens) pasture ad lib. 1. protein and energy deposition in response to abomasal infusion of casein and methionine. | 1981 | 7317346 | |
motility of the reticulum and rumen of sheep given juice-extracted pasture. | 1. sheep were fed on different diets of juice-extracted herbage to determine what effect juice-extraction had on reticulo-rumen motility. 2. the frequency of a and b sequences of contraction of the reticulo-rumen were recorded during eating, rumination and inactivity for continuous periods of 24-72 h by using integrated electromyograms obtained from electrodes implanted in the musculature of the reticulum and cranial dorsal rumen. 3. animals were fed on herbage in which approximately 200 g/kg dr ... | 1982 | 7059570 |
protein metabolism in growing lambs given fresh ryegrass (lolium perenne)--clover (trifolium repens) pasture ad lib. 2. endocrine changes, glucose production, and their relationship to protein deposition and the partition of absorbed nutrients. | 1. glucose metabolism and changes in the concentrations of several hormones in jugular plasma were measured in growing lambs fed on fresh pasture ad lib. one group of lambs acted as control while the second received a continuous abomasal infusion supplying 44 g sodium caseinate+0.5 g l-methionine/d. 2. hormone concentrations were determined by radioimmunoassay procedures and glucose irreversible loss measured from continuous infusion of d-[u-14c]glucose. 3. protein infusion increased plasma conc ... | 1982 | 7066291 |
nuclear changes induced by the nematodes xiphinema diversicaudatum and longidorus elongatus in root-tips of perennial ryegrass, lolium perenne. | the dna content and size of individual nuclei from galls of perennial ryegrass root-tips induced by x. diversicaudatum and l. elongatus were measured. feeding by x. diversicaudatum increased the dna content of the nuclei by varying amounts. no regular doubling pattern of the dna content was discernible. the dna values varied up to between 32-64c. generally the size of the nuclei was not increased, although some were larger than control nuclei. the modified nuclei probably have an altered metabol ... | 1982 | 7129956 |
effect of intravenous supplements of l-methionine on milk yield and composition in cows given silage-cereal diets. | eight cows in mid lactation and receiving a diet of perennial ryegrass silage and barley (70:30 on a dm basis) were given intravenous supplements of l-methionine (8 g/d). the methionine treatment had no significant effect on milk yield, protein content or lactose content, but increased milk fat content and yield by approximately 10% (p less than 0.05). | 1982 | 6804549 |
allergenic cross-reactivity of cytochromes c of kentucky bluegrass and perennial ryegrass pollens. | the allergenic cross-reactivity of cytochromes c isolated from kentucky bluegrass (kbg) and ryegrass (rg) pollens was demonstrated by the finding that both cytochromes elicited pca reactions of comparable titers in rats sensitized with murine reaginic antiserum to either kbg or rg cytochrome c. however, allergenic differences were revealed at limiting doses of the cytochromes c; under these conditions rg cytochrome c elicited pca reactions only with the homologous reaginic serum, whereas kbg cyt ... | 1982 | 6298610 |
copper metabolism in growing sheep given kale (brassica oleracea) and ryegrass (lolium perenne)-clover (trifolium repens) fresh forage diets. | kale (brassica oleracea) and ryegrass (lolium perenne)-clover (trifolium repens) pasture grown under similar soil conditions were grazed in the vegetative state by growing lambs of 23.6 kg initial live weight for 24 weeks. forty-eight lambs grazed each forage. the kale and pasture contained respectively 4 and 14 mg copper/kg dry matter (dm), 7.2 and 3.1 g total sulphur/kg dm and 0.4 and 1.1 mg molybdenum/kg dm. subcutaneous injections of cu (12 mg) were given to half the animals grazing each for ... | 1983 | 6311244 |
reverse enzyme immunoassay for the determination of lolium perenne ige antibodies. | a microplate enzyme-immunoassay technique for the detection of specific anti-lolium perenne ige antibodies is described. this method consists of coating polystyrene wells with goat antihuman ige (epsilon chain specific) and late selecting the ige from the patients serum in order to finally reveal the bound specific ige by means of a crude l. perenne extract conjugated with peroxidase. the optimal conditions necessary to achieve maximum sensitivity and specificity using this system have been stud ... | 1983 | 6347907 |
iodine metabolism and thyroid hormone relationships in growing sheep fed on kale (brassica oleracea) and ryegrass (lolium perenne)-clover (trifolium repens) fresh-forage diets. | 1983 | 6830751 | |
immunochemical characterization of a high molecular weight basic allergen (hmba) of rye grass (lolium perenne) pollen. | a high molecular weight basic allergen (hmba) was isolated from the mixture of non-dialysable components of the aqueous extract of defatted rye grass pollen by a combination of gel filtration and isoelectrofocusing, hmba, a glycoprotein of mol. wt 56,800 (17% carbohydrate) contained all naturally occurring amino acids. a hyperimmune rabbit anti-hmba serum gave only a single precipitin band with the crude extract of the rye grass pollen in crossed immunoelectrophoresis. thus, it was concluded tha ... | 1983 | 6865958 |
development of monoclonal mouse antibodies specific for allergenic components in ryegrass (lolium perenne) pollen. | the development of monoclonal mouse antibodies against ryegrass (lolium perenne) pollen allergens is described. hybridoma colonies secreting antibodies specific for allergenic components were detected using an enzyme-linked immunosorbent assay. positive colonies were cloned and expanded. the pollen components with which the monoclonal antibodies interact were identified and characterised following sodium dodecyl sulphate polyacrylamide gel electrophoresis and electrophoretic transfer to nitrocel ... | 1983 | 6618685 |
alkaloids in ergot found on different gramineae in the netherlands. | the alkaloid content and the composition of the alkaloid complex of thirteen samples of ergot sclerotia from different gramineous host species collected in the netherlands were investigated. two samples collected in france were also examined. ergot of glyceria fluitans (l.) r.br. did not contain alkaloids. the total alkaloid content of ergot found on the other wild grasses was more than 0.2%. the ratio between the contents of water soluble and water insoluble alkaloids of the investigated ergot ... | 1983 | 6646990 |
[chemical and immunologic study of pollen from the gramineous plant lolium perenne]. | in order to study the chemical composition and immunologic reactivity of an extract of rye grass pollen (rgp), aliquots of this antigen were passed through sephadex g-50 and deae-cellulose ion-exchange columns. this method yielded 7 proteins and 10 sugars, whose quantities were determined by absorbance in a lkb uvicrd spectrophotometer at 280 nm and 470 nm respectively. protein contents were also recorded by the lowry method and the micro-kjehldal technique. adult albino rabbits were immunized w ... | 1983 | 6660173 |
a report on the consumption, composition and nutritional adequacy of a mixture of lush green perennial ryegrass (lolium perenne) and cocksfoot (dactylis glomerata) fed ad libitum to thoroughbred mares. | non-pregnant thoroughbred mares were stabled and subjected to 2 trials, each 24 h in duration, to establish their total consumption of a mixture of freshly cut, lush green perennial ryegrass (lolium perenne) and cocksfoot (dactylis glomerata) in approximately equal proportions; and to compare the total intake of crude protein, calcium, phosphorus and mass of the grass mixture on a dry matter basis with their daily nutritional requirements. the body mass of each mare was calculated at the commenc ... | 1983 | 6655653 |
rooting pattern and water relations of three pasture grasses growing in drying soil. | seedlings of perennial ryegrass (lolium perenne l. s23), cocksfoot (dactylis glomerata l. s37) and timothy (phleum pratense l. s48) were rooted into tubes of soil and plants were either watered well or remained unwatered for a period of seven days. measurements were made of root water relations and of growth of roots and shoots. root turgor of dactylis was larger than that of lolium and phleum. as a result of accumulation of solutes, turgor of dactylis and lolium was maintained as the soil dried ... | 1983 | 28310581 |
wide hybridization experiments in cereals. | wide hybridization is a useful tool in plant breeding, but little is known about its possible range. for the cereals, wheat, barley and rye, this was tested with 15 different species of the poaceae and panicoideae. embryo formation could be obtained with agropyron repens, alopecurus agrestis, dactylis glomerata, festuca glauca, hordeum bulbosum, lolium perenne, pennisetum americanum, and zea mays. as well, haploid as diploid embryos occurred. new embryo culture techniques should enable these emb ... | 1984 | 24257639 |
the role of condensed tannins in the nutritional value of lotus pedunculatus for sheep. 2. quantitative digestion of carbohydrates and proteins. | primary growth vegetative lotus pedunculatus containing 46 and 106 g/kg dry matter (dm) of total condensed tannin and 3 and 14 g/kg dm of free condensed tannin, was cut and fed fresh at hourly intervals (750 g dm/d) to sheep fitted with permanent cannulas into the rumen and duodenum. low- and high-tannin lotus contained respectively 41.3 and 31.6 g total nitrogen/kg dm and 132 and 152 g lignin/kg dm. the two forms of lotus were similar in carbohydrate composition. nutrient intake was recorded an ... | 1984 | 6722090 |
[ryegrass staggers--lolium perenne (english ryegrass)]. | 1984 | 6484925 | |
cross-reactions among grass pollen allergens. | we studied cross-reactions of pollen extracts from the grasses phleum pratense, lolium perenne, poa pratensis and festuca pratensis. we used immunoelectrophoretic techniques (tandem-cie, absorption in situ) and rast inhibition, which allowed us to detect the high antigenic and allergenic relationships of the single grass pollens. phleum pratense, however, revealed antigenic and allergenic differences as compared to the other 3 grasses. | 1984 | 6084953 |
comparative digestion, rumen fermentation and kinetics of forage diets by steers and wethers. | four rumen fistulated wether and beef steers were used to evaluate differences in dry matter digestibility (dmd) between cattle and sheep. they were fed either perennial ryegrass or switchgrass hay at an ad libitum or restricted level for four experimental periods. significant ruminant species x forage and ruminant species x level of intake (p less than .05) interactions were observed for digestible dry matter. the steers digested the switchgrass 7 percentage units greater than the wethers while ... | 1984 | 6086564 |
the physical digestion of perennial ryegrass (lolium perenne) and white clover (trifolium repens) in the foregut of sheep. | sheep were fed once daily with 300 or 600 g dried perennial ryegrass (lolium perenne) or white clover (trifolium repens). total rumen contents were sampled once daily at various intervals up to 24 h after feeding for 6 d. total weight of fresh rumen contents and dry matter (dm), organic matter and particulate matter concentrations were measured and the particle size distribution of rumen contents was analysed using a wet sieving technique. there was a similar exponential loss of dm from the rume ... | 1984 | 6089866 |
transmethylation reactions in human basophils induced by anti-ige or specific antigen. | the activation of methyl transferases in the membrane of human basophilic leukocytes was measured by incorporation of 3h-methyl groups into phospholipids. basophils of healthy individuals were activated by anti-ige, those of pollen allergic patients by the specific antigen, lolium perenne extract. basophil-rich fraction was obtained from peripheral blood by centrifugation in a gradient of ficoll/metrizamide (g = 1.085). after incubation with anti-ige an increase in the 3h-methyl incorporation wa ... | 1984 | 6199310 |
[identification of allergens in 5 grasses using crossed radioimmunoelectrophoresis (crie)]. | using crossed radioimmunoelectrophoresis ( crie ) aqueous extracts from pollen of phleum pratense , lolium perenne, poa pratensis , festuca pratensis and alopecurus pratensis were investigated for allergen composition. we detected between 24 and 32 antigens. employing sera from 11 patients with well established hay fever, ige binding could be demonstrated in 15 out of 28 antigens in phleum pratense , 13 out of 32 in lolium perenne, 14 out of 26 in poa pratensis , 12 out of 24 festuca pratensis a ... | 1984 | 6233859 |
the digestion of fresh perennial ryegrass (lolium perenne l. cv. melle) and white clover (trifolium repens l. cv. blanca) by growing cattle fed indoors. | 1. pure swards of perennial ryegrass (lolium perenne l. cv. melle) or white clover (trifolium repens l. cv. blanca) were harvested daily at three and two stages of growth respectively, and offered to housed cattle. the grass diets comprised primary growth (may) and two later regrowths of contrasting morphology (i.e. leaf: stem values of 1.54 and 2.84 respectively), and were characterized by high contents of water-soluble carbohydrate and neutral-detergent fibre and comparable in vitro dry matter ... | 1985 | 3870696 |
[immediate hypersensitivity to fruits and vegetables and pollenosis]. | various works have been published, mainly by scandinavian authors, in which a partial immunological identity has been found to exist between birch pollen and hazelnut and apple. however, our attention has been particularly drawn to the high proportion of people who are allergic to the apple and other fruits, which in some cases has been as much as 50 and 70%, and in whom an oral pruritus alone is considered sufficient a symptom of allergies to certain foodstuffs. however due to the fact that the ... | 1985 | 4036764 |
stabilizing effect of epsilon-aminocaproic acid on allergenic extracts. | the effect of epsilon-aminocaproic acid (eaca) on the degradation of an aqueous lolium perenne extract was studied by intracutaneous tests and by rast inhibition. extracts for skin testing stored at 4 degrees c for 12 months and at 37 degrees c for 6 weeks were significantly protected from degradation by addition of 0.1 mol/l of eaca before storage. extracts were stored for rast inhibition at 4 degrees c for 6 months and at 37 degrees c for 7 days. shelf life was twofold to threefold increased w ... | 1985 | 4056242 |
endocrine regulation of metabolism in sheep given kale (brassica oleracea) and ryegrass (lolium perenne)-clover (trifolium repens) fresh-forage diets. | diets of fresh kale (brassica oleracea) and ryegrass (lolium perenne)-clover (trifolium repens) herbage were fed to growing sheep in three experiments. in expts 1 and 3 the sheep were confined indoors and fed at hourly intervals, and all were given supplementary iodine to counteract kale goitrogens. lambs grazed the two forages for 24 weeks in expt 2, with and without intramuscular injections of iodized oil. the kale and herbage contained respectively 11 and less than 0.1 g s-methyl-l-cysteine s ... | 1985 | 4063301 |
nitrogen digestion and metabolism in sheep consuming diets containing contrasting forms and levels of n. | nitrogen kinetics were studied in six sheep (45-55 kg live weight) consuming either a high-n grass silage or a low-n dried grass made from swards of perennial ryegrass (lolium perenne). the diets were fed hourly at a level of 600 g dry matter/d and supplied 19.5 and 11.0 g n/d respectively. the amounts of organic matter (om) consumed and flowing at the duodenum and ileum and excreted in the faeces were similar (p greater than 0.05) with both diets. each diet supplied 23 g digestible om/d per kg ... | 1985 | 4063302 |
the role of condensed tannins in the nutritional value of lotus pedunculatus for sheep. rates of body and wool growth. | lotus pedunculatus (cv. grasslands maku) grown on acid low-fertility soil and containing high concentrations of condensed tannin (76-90 g/kg dry matter (dm] was grazed by growing sheep for 31-42 d periods in three experiments. in expt 2 an additional group of lambs grazed areas oversown with white clover (trifolium repens) and red clover (trifolium pratense). lambs were transferred from grazing ryegrass (lolium perenne)-white clover straight on to lotus in all experiments (unconditioned sheep). ... | 1985 | 4063306 |
estimation of microbial nitrogen in nylon-bag residues by feed 15n dilution. | rapeseed (brassica napus), barley grain, ryegrass (lolium perenne) and barley straw were labelled with 15n as an internal marker. the dilution of 15n was used to estimate microbial n (rmn15n) in the feed residues in nylon bags incubated in the rumen for 5, 12 and 24 h. for comparative purposes, diaminopimelic acid (dap) content of the residues was also determined and rumen microbial n (rmndap-n) in the feed residues calculated using dap as a bacterial marker. the influence of two bag pore sizes ... | 1985 | 4063332 |
glucose and protein metabolism during late pregnancy in triplet-bearing ewes given fresh forages ad lib. 1. voluntary intake and birth weight. | ewes of the booroola x romney genotype carrying triplet lambs were given fresh forages ad lib. in late pregnancy. in expt 1, groups of three ewes were given kale (brassica oleracea), perennial ryegrass (lolium perenne) or perennial ryegrass (0.75)-barley (0.25). in expt 2, groups of two or three ewes were given fresh perennial ryegrass and infused into the abomasum with iso-energetic quantities of casein and glucose in a 2 x 2 factorial arrangement. post-lambing ewe live weights were 40-50 kg. g ... | 1985 | 4063334 |
studies on the host range, biology, and pathogenicity of punctodera punctata infecting turfgrasses. | punctodera punctata completed its life cycle on poa annua (annual bluegrass), p. pratensis (merion kentucky bluegrass), lolium perenne (perennial ryegrass), and festuca rubra rubra (spreading fescue). minimum time for completion of a life cycle from second-stage juvenile to mature brown cyst was 40 days at 22-28 c. inoculation by single juveniles indicated that reproduction was most likely by amphimixis. infestation levels of 50 or 500 juveniles/250 cm(3) soil did not affect top dry weight, root ... | 1985 | 19294077 |
phytotoxic properties of nordihydroguaiaretic acid, a lignan fromlarrea tridentata (creosote bush). | the phytotoxic properties of nordihydroguaiaretic acid (ndga) isolated from creosote bush,larrea tridentata (zygophyllaceae), were examined. ndga dramatically reduces the seedling root growth of barnyard grass, green foxtail, perennial ryegrass, annual ryegrass, red millet, lambsquarter, lettuce, and alfalfa, and reduces the hypocotyl growth of lettuce and green foxtail. it has no effect on the germination of lettuce seeds. ndga almost certainly contributes to the observed allelopathic nature of ... | 1985 | 24311094 |
cerebellar disease in an adult cow. | this is the report of clinical signs and lesions of a cerebellar disorder in an adult four year old limousin cow grazing perennial ryegrass (lolium perenne). the most striking histopathological lesion was a marked paucity of purkinje cells throughout the cerebellum. | 1986 | 17422607 |
metabolism of lactic acid isomers in the rumen of silage-fed sheep. | 1. four mature sheep were offered perennial ryegrass (lolium perenne, cv. s23) silage (885 g dry matter/d) at hourly intervals. the silage was well fermented with a ph of 4.0, a lactic acid content of 139 g/kg dry matter and an organic matter digestibility of 0.766. 2. continuous intraruminal infusions of 14c-labelled sodium salts of [u-14c]acetic acid, [2-14c]propionic acid, [2-14c]butyric acid and d- and l-[u-14c]lactic acid and an intravenous infusion of [u-14c]glucose were made on separate o ... | 1986 | 3676167 |
the efficiency of energy utilization in growing cattle consuming fresh perennial ryegrass (lolium perenne cv. melle) or white clover (trifolium repens cv. blanca). | 1. twenty friesian steers (225 kg live weight) were fed on mid- (m) (june-july) and late- (l) (august-september) season crops of either fresh perennial ryegrass (lolium perenne cv. melle) (g) or white clover (trifolium repens cv. blanca) (c). each of the forage diets was offered at three restricted planes of nutrition above maintenance to compare the effect of forage species on the efficiency of energy utilization. all diets were harvested daily from swards of regrowth forage of intended equival ... | 1986 | 3676184 |
microbially corrected amino acid composition of rumen-undegraded feed protein and amino acid degradability in the rumen of feeds enclosed in nylon bags. | 1. in the previous work (varvikko & lindberg, 1985), 15n-labelled rapeseed (brassica napus), barley, ryegrass (lolium perenne) and barley straw were incubated in the rumen in nylon bags for 5, 12 and 24 h and microbial nitrogen in the residues was quantified using the feed 15n-dilution method. in the present study, residual amino acids (aa) of these feeds were analysed, and microbially corrected aa of feed origin (feed aa) were estimated as the difference between total residual aa and respective ... | 1986 | 3676189 |
effect of forage species and season on nutrient digestion and supply in grazing cattle. | 1. a total of twenty friesian steers were grazed on pure swards of either perennial ryegrass (lolium perenne cv. melle) or white clover (trifolium repens cv. blanca) from may to late august to examine the effect of forage species and season on nutrient digestion and supply. within each forage species, two daily allowances of forage (i.e. 30 and 60 g dry matter (dm)/kg live weight) were given, and nutrient flow into the small intestine was measured on thirteen separate occasions (viz. seven grass ... | 1986 | 3676197 |
the effect of forage species and stage of harvest on the processes of digestion occurring in the rumen of cattle. | 1. pure swards of perennial ryegrass (lolium perenne l. cv. melle) or white clover (trifolium repens l. cv. blanca) were harvested daily as either primary growth (may-june) or mid- (july) and late- (august-september) season 4-week regrowths and offered to friesian steers at two levels of feed allowance (18 and 24 g dry matter (dm)/kg live weight), to examine the effect of forage species and stage of harvest on nutrient digestion and supply. 2. the early- and mid-season grasses had low nitrogen ( ... | 1986 | 3676223 |
the effect of digestibility and forage species on the removal of digesta from the rumen and the voluntary intake of hay by sheep. | 1. the characteristics of digestion, passage and rumen fill of three hays: early- and late-cut perennial ryegrass (lolium perenne cv. endura) and white clover hay (trifolium repens cv. blanca and pronitro) were studied using six rumen-cannulated sheep fed at a restricted level of intake (18 g dry matter (dm)/kg live weight (lw) per d), in a two 3 x 3 latin square design. 2. voluntary intake of the same diets was measured using a further six non-cannulated sheep in a similar design. 3. rate of di ... | 1986 | 3676225 |
the effect of supplementation with maize starch and level of intake of perennial ryegrass (lolium perenne cv. endura) hay on the removal of digesta from the rumen of sheep. | 1. eight wether sheep were offered a diet of perennial ryegrass (lolium perenne cv. endura) hay once daily at two levels of intake (11 and 16.5 g dry matter (dm)/kg live weight (lw] with or without maize starch (175 g dm/kg hay dm). the experiment consisted of four periods, each lasting 32 d. rates of digestion of the hay were measured by incubation in dacron bags and rates of passage using chromium-mordanted hay. rumen pool sizes of dm, organic matter and fibre were measured by emptying the rum ... | 1986 | 3676226 |
physicochemical and immunochemical characterization of allergenic proteins from rye-grass (lolium perenne) pollen prepared by a rapid and efficient purification method. | three fractions of rye-grass (lolium perenne) pollen extract have been isolated by preparative isoelectric focusing (i.e.f.) and characterized in terms of physicochemical and immunochemical properties. the purified components were designated 'r7' and 'r14' on the basis of their positions in relation to other rye-grass pollen extract components on sds/polyacrylamide-gel electrophoresis and their apparent molecular masses were assessed as 31 and 11 kda respectively. on i.e.f., r14 split into two c ... | 1986 | 3718469 |
a study of the human immune response to lolium perenne (rye) pollen and its components, lol p i and lol p ii (rye i and rye ii). i. prevalence of reactivity to the allergens and correlations among skin test, ige antibody, and igg antibody data. | in a stratified random sample of 320 white adults, the prevalence of puncture skin test positivity (st +) to lolium perenne (rye grass)-pollen extract (lpe) was 16%. fifteen percent of all subjects (or 84% of subjects classified lpe ige antibody positive [ab +]) was classified ige ab + to highly purified lol p i (rye i), and 4% of all subjects (or 26% of subjects classified lpe ige ab +) was classified ige ab + to highly purified lol p ii (rye ii). these data and similar results obtained in an a ... | 1986 | 3782681 |
the use of monensin or formaldehyde to control the digestion of the nitrogenous constituents of perennial ryegrass (lolium perenne cv. melle) and white clover (trifolium repens cv. blanca) in the rumen of cattle. | pure swards of perennial ryegrass (lolium perenne cv. melle) and white clover (trifolium repens cv. blanca) were harvested daily as primary growth (grass in may, clover in june) or regrowths (clover only in july) and offered, in the long form, to growing cattle at a feeding rate of 22 g dry matter/kg live weight. with each forage, two treatments were compared with the untreated forage (c): monensin (100 g active ingredient/kg, 250 mg/d) addition to the rumen (treatment m) or formaldehyde (30 g/k ... | 1987 | 3801385 |
activation of human serum complement with allergens. i. generation of c3a, c4a, and c5a and induction of human neutrophil aggregation. | to understand the relevance of allergens in generation of c3a, c4a, and c5a in normal human serum, we studied extracts of several allergens (house dust, house dust mite, aspergillus fumigatus, and perennial ryegrass). known complement activators zymosan and endotoxin were used as controls. generation of c3a, c4a, and c5a (determined by radioimmunoassays) occurred with extracts of house dust and aspergillus more than with house dust mite and ryegrass. anaphylatoxins were produced both in dose and ... | 1987 | 3496373 |
digestibility, intake and mineral utilization of combinations of grasses and legumes by lambs. | defined mixtures of two grass (orchardgrass, perennial ryegrass) and two legume (alfalfa, red clover) hays, harvested in two cuttings in 2 yr, were fed in digestibility, intake and mineral balance trials to wether lambs. each mixture was fed in ad libitum amounts to six crossbred wether lambs weighing 35 to 45 kg. the effect of increasing proportion of legume in mixtures on dry matter digestibility (dmd), neutral detergent fiber digestibility (dndf) and digestible dry matter intake (ddmi) differ ... | 1987 | 3597188 |
a study of the human immune response to lolium perenne (rye) pollen and its components, lol p i and lol p ii (rye i and rye ii). ii. longitudinal variation of antibody levels in relation to symptomatology and pollen exposure and correction of seasonally elevated antibody levels to basal values. | this study used a standardized, dialyzed, lolium perenne (ryegrass) pollen extract and two of its well-characterized components, lol p i (rye i) and lol p ii (rye ii), to characterize the longitudinal variation of both ige and igg antibody (ab) levels, as well as total serum ige levels, in 20 grass-allergic subjects followed for 13 months. ab levels declined toward a basal level just before, and increased just after, the grass-pollination season, returning to the same basal level just before the ... | 1987 | 3680810 |
the prevalence of allergic skin test reactivity to eight common aeroallergens in the u.s. population: results from the second national health and nutrition examination survey. | immediate hypersensitivity skin tests to eight select allergens were performed on a sample (n = 16,204) of the civilian noninstitutional population of the united states, 6 to 74 years of age, in the second national health and nutrition examination survey (nhanes ii). the eight allergens were house dust, cat, dog, alternaria, mixed giant/short ragweed, oak, perennial ryegrass, and bermuda grass. skin test reactivity was defined as a mean erythema diameter greater than or equal to 10.5 mm at the 2 ... | 1987 | 3680811 |
immunochemical studies of lolium perenne (rye grass) pollen allergens, lol p i, ii, and iii. | it was reported earlier that human immune responses to three perennial rye grass (lolium perenne) pollen allergens, lol p i, ii, and iii, are associated with histocompatibility leukocyte antigen (hla)-dr3. rye-allergic people are often concordantly sensitive to all three of these allergens. since earlier studies suggested that these antigens are non-cross-reactive, their immunologic relatedness by double antibody radioimmunoassay (daria) was studied in order to understand further the immunochemi ... | 1987 | 3693899 |
distribution of a major allergen of rye grass (lolium perenne) pollen between other grass species. | polyacrylamide gel electrophoresis in sodium dodecyl sulphate (sds) followed by protein staining has shown that extracts from 11 different grass pollens contained proteins with similar molecular weights to that of the allergen r7 from rye grass (lolium perenne) pollen extract (i.e. 29,000-31,000 daltons). western blotting and detection with polyclonal (rabbit) antibodies raised against the purified r7 (rye i) allergen indicated that these proteins were antigenically related and their allergenic ... | 1987 | 2437060 |
an investigation of human immune response to perennial ryegrass (lolium perenne) pollen cytochrome c (lol p x). | cytochrome c (lol p x) was purified from the pollen of perennial ryegrass (lolium perenne). it was used in a sensitive double-antibody radioimmunoassay to determine specific igg and ige antibody (ab) levels in the sera of grass-allergic human subjects and the relationship between immune responsiveness and hla type. a total of 139 subjects were studied, including 63 subjects treated by grass immunotherapy and 76 untreated subjects. the sera of only four subjects were found to contain anticytochro ... | 1987 | 3038988 |
effects of ambient levels of air pollution on grass swards subjected to different defoliation regimes. | the urgent need to evaluate effects of air quality on plant growth in the field is discussed in relation to the difficulties which field-based studies pose in the interpretation of data. an initial study is described which aimed to overcome some of these difficulties and the data are discussed within the remaining limitations. the productivity of pure swards of lolium perenne l. and dactylis glomerata l., subjected to defoliation at different times of the year, was studied at field sites with di ... | 1987 | 15092760 |
effects of fungal endophytes on the seed and seedling biology of lolium perenne and festuca arundinacea. | many grasses are infected by endophytic fungi that grow intercellularly in leaves, stems, and flowers and are transmitted maternally by hyphal growth into ovules and seeds. the seed biology and seedling growth of endophyte-infected and uninfected perennial ryegrass (lolium perenne) and tall fescue (festuca arundinacea) were investigated under controlled environmental conditions. the percentage of filled seeds produced by infected tall fescue was over twice of uninfected tall fescue; infected and ... | 1987 | 28311516 |
cross-reactive and unique grass group i antigenic determinants defined by monoclonal antibodies. | as part of a study to probe the immunochemical basis for allergenic cross-reactivity among grass pollens, a series of useful reagents has been prepared. the major grass-pollen allergen, designated group i (gpi), was isolated from five grass pollens (meadow fescue, june grass, sweet vernal grass, redtop grass, and perennial ryegrass). the purified gpi antigens were used to immunize individual groups of balb/c mice. a total of 123 hybridoma-derived anti-gpi monoclonal antibodies (mabs) was produce ... | 1987 | 2434551 |
the effect of date of cut and barley substitution on gain and on the efficiency of utilization of grass silage by growing cattle. 1. gains in live weight and its components. | 1. a primary growth of perennial ryegrass (lolium perenne) was cut early or late to produce silages of high and low digestibility. the crops were wilted for 2-4 h and preserved with formic acid at 2.4 litres/t fresh weight. the resulting silages were well preserved with a ph of 3.9 and 3.8, lactic acid content of 108 and 73 g/kg dry matter (dm) and total nitrogen content of 24.6 and 18.4 g/kg dm for early- and late-cut silage respectively. 2. forty-two british friesian male castrates (steers) in ... | 1988 | 3196672 |
the effect of date of cut and barley substitution on gain and on the efficiency of utilization of grass silage by growing cattle. 2. nutrient supply and energy partition. | 1. the effect of harvesting date of perennial ryegrass (lolium perenne) on the nutritive value of the resultant silage and the effect of substitution of late-cut silage with barley was examined in growing cattle. the diets comprised early-cut (h) and late-cut (l) silage offered alone or with 280 (lc1) or 560 (lc2) g rolled barley/kg total dry matter (dm) substituted for late-cut silage. 2. both silages were prepared with the addition of formic acid (850 milligram; 2.4 litres/t fresh weight) to a ... | 1988 | 3196673 |
protein metabolism in the rumen of silage-fed steers: effect of fishmeal supplementation. | 1. ryegrass (lolium perenne cv. cropper) silage was given to four friesian steers (initial live weight (lw) 172 kg) alone or with a fishmeal supplement (150 g fresh weight/kg silage dry matter (dm)) in a balanced two-period change-over design. the dietary components were the same as those used in a recent experiment by gill et al. (1987). all diets were offered hourly at 24 g dm/kg lw. 2. fishmeal supplementation increased dietary nitrogen intake (p less than 0.01) and significantly increased th ... | 1988 | 3196674 |
association of hla-dr3 with human immune response to lol p i and lol p ii allergens in allergic subjects. | associations between hla type and ige or igg antibody (ab) responses to two well-characterized, antigenetically non-crossreactive components of lolium perenne (rye grass) pollen extract, lol p i (rye i) and lol p ii (rye ii) were studied in two groups of skin-test positive (st+) caucasoid adults. by both nonparametric and parametric statistical methods, significant associations were found between ab responses to both lol i and lol ii and the possession of hla-dr3. in view of the well-known assoc ... | 1988 | 3261461 |
in vitro synthesis of ige by human peripheral blood leucocytes: vi. seasonal variations in synthesis of rye pollen (lolium perenne) specific ige. | fractionation by percoll density centrifugation of peripheral blood leucocyte cells, from atopic subjects with seasonal hay fever, unmasked ige-b cell populations whose individual capacities to synthesize ige in vitro were obscured in cultures of unfractionated b cells. b cell cultures from all six subjects in the study released rye pollen-specific ige during the 6 days of culture, but actual synthesis was significant only in october, the pollen season. synthesis in october occurred most frequen ... | 1988 | 3263316 |
standardization of rye-grass pollen (lolium perenne) extract. an immunochemical and physicochemical assessment of six candidate international reference preparations. | six candidate extracts of lolium perenne (rye-grass) pollen have been studied in 6 laboratories using a variety of immunochemical and physicochemical techniques. radioallergosorbent test inhibition, crossed immunoelectrophoresis, crossed radio-immunoelectrophoresis, sodium dodecyl sulphate-polyacrylamide gel electrophoresis combined with immunoblot, thin-layer isoelectric focusing and enzyme-linked immunosorbent assay inhibition were used to evaluate each of the coded extracts. the source materi ... | 1988 | 3372043 |
large particle breakdown by cattle eating ryegrass and alfalfa. | the proportion of large particles (lp) broken down to small, insoluble particles by primary mastication (eating), rumination, digestion and detrition (rubbing) was determined for separated leaf and stem fractions of perennial ryegrass (lolium perenne) and alfalfa (medicago sativa) fed to cattle cannulated at the esophagus. large particles were defined as those particles retained during wet sieving on a screen with an aperture of 1.18 mm. reduction in weight of particles caused by solubilizing or ... | 1988 | 3378956 |
the digestion of perennial ryegrass (lolium perenne cv. melle) and white clover (trifolium repens cv. blanca) by grazing cattle. | 1. pure swards of perennial ryegrass (lolium perenne cv. melle) as a primary growth (may), trimmed primary growth (early june) and regrowth (late june), and white clover (trifolium repens cv. blanca) as a mature primary growth (july) and vegetative regrowth (august), were grazed by twelve friesian steers (mean body-weights throughout experiment 152-231 kg) at daily allocation rates of forage which provided dry matter (dm) intakes ranging from 28 to 36 g/kg body-weight). 2. total nitrogen content ... | 1988 | 3408697 |
study of the epitope structure of purified dac g i and lol p i, the major allergens of dactylis glomerata and lolium perenne pollens, using monoclonal antibodies. | the use of mab allowed us to further analyze the cross-reactivity between purified dac g i and lol p i, the major allergens of dactylis glomerata (cocksfoot) and lolium perenne (rye grass), respectively. it was first shown, using ief, followed by immunoprinting, that serum ige antibodies from most grass-sensitive patients recognize both dac g i and lol p i. second, three different anti-lol p i mab, 290a-167, 348a-6, and 539a-6, and one anti-dac g i mab, p3b2 were all shown to react with dac g i ... | 1988 | 2460536 |
mapping of lol p i allergenic epitopes by using murine monoclonal antibodies. | murine monoclonal antibodies (mabs) against three non-overlapping epitopes of lol p i allergen were previously produced and subsequently used for purification of the allergen. in the present study, these mabs were further characterized, and the biological activity of the purified allergen assessed. the three mabs were of the igg isotype and carried a kappa light chain. their affinity constants were in the range of 7.4-15.1 x 10(-9) mol/l. purified lol p i kept its biological activity, as shown b ... | 1989 | 2481823 |
molecular genetics of human immune responsiveness to lolium perenne (rye) allergen, lol p iii. | lol p ii and iii are each about 11-kd protein allergens from the pollen of lolium perenne (rye grass). we have found that human immune responses (ige and igg antibodies) to both proteins are significantly associated with hla-dr3. in addition, the two proteins are cross-reactive with the antibodies in many human sera (about 84% human sera showed the cross-reactivity). we have determined greater than 90% of the amino acid sequences of the two proteins and found that they are at least 54% homologou ... | 1989 | 2496037 |
complete primary structure of a lolium perenne (perennial rye grass) pollen allergen, lol p iii: comparison with known lol p i and ii sequences. | the complete amino acid sequence of a lolium perenne (rye grass) pollen allergen, lol p iii, determined by the automated edman degradation of the protein and its selected fragments, is reported in this paper. cleavage by enzymatic and chemical techniques established unambiguously the sequence for this 97-residue protein (mr = 10,909), which lacks cysteine and shows no evidence of glycosylation. the sequence of lol p iii is very similar to that of another l. perenne allergen, lol p ii, which was ... | 1989 | 2605214 |
particle breakdown and rumen digestion of fresh ryegrass (lolium perenne l.) and lucerne (medicago sativa l.) fed to cows during a restricted feeding period. | 1. five rumen-fistulated cows were given freshly cut ryegrass (lolium perenne l.) and lucerne (medicago sativa l.), on separate occasions, to compare rates of particle breakdown and aspects of rumen fermentation. 2. cattle were required to consume their daily allowance (offered ad lib.) in two 2 h feeding periods daily. 3. during the first 2 h feeding period, dry matter (dm) intakes of ryegrass (4.75 kg) and lucerne (4.85 kg) were similar. eating reduced 46% of ryegrass and 61% of lucerne to a s ... | 1989 | 2706235 |
human immune responsiveness to lolium perenne pollen allergen lol p iii (rye iii) is associated with hla-dr3 and dr5. | a well-characterized allergen of lolium perenne (perennial rye grass) pollen, lol p iii, has been used as a model antigen to study the genetic control of the human immune response. associations between hla type and ige or igg antibody (ab) responsiveness to lol p iii were studied in two groups of skin-test-positive caucasoid adults (n = 135 and 67). we found by nonparametric and parametric analyses that immune responsiveness to lol p iii was significantly associated with hla-dr3 and dr5. no asso ... | 1989 | 2715056 |