Publications

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studies on bacterial nutrition : iv. effect of plant tissue upon growth of pneumococcus and streptococcus.in previous papers it has been shown that unheated plant tissue, in the form of potato, contains the two factors necessary for the growth of organisms of the hemoglobinophilic group. further studies (5) confirmed these findings and showed that yellow and white turnip, carrot, beet, parsnip, and sweet potato can replace blood in the cultivation of bacillus influenzae. in the present paper it has been shown that vegetable tissues also greatly facilitate and stimulate the growth of other organisms ...192319868784
the partial purification and properties of antibiotic substances from the sweet potato plant (ipomoea batatas). 194918138318
the partial purification and properties of antibiotic substances from the sweet potato plant (ipomoea batatas). 194916695758
sweet potatoes (ipomoea batatas). 194918132268
the effect of carbon monoxide on respiration in the sweet potato, ipomoea batatas lan. 195313056600
utilization of sweet potato starch by rats and its effect on the digestion of dietary protein. 195513278779
transmission of internal cork of sweet potato by the cotton aphid, aphis gossypii glover. 195817781179
effect of synthetic polylysine on fungi.the synthetic, basic poly-alpha-amino acid, polylysine, had antifungal activity against plant pathogens (three strains of fusaria, three isolates of verticillia, and ceratocystis fimbriata) and against the human pathogens (trichophyton mentagrophytes, t. rubrum, and candida albicans) in vitro. it inhibited penetration of ceratocystis fimbriata on sweet potato slices. polylysine inhibited the infection of tomato cuttings by flsarium oxysporum f. lycopersici, but it was also toxic to the plants.196017747266
analytical study of ipomeamarone & chlorogenic acid alterations in sweet potato roots infected by ceratocystis fimbriata. 196116655482
effects of thermoperiodism on tuber formation in ipomoea batatas under controlled conditions. 196116655574
pattern of carbohydrate breakdown in sweet potato roots infected with ceratocystis fimbriata. 196216655711
analytical study of umbelliferone and scopoletin synthesis in sweet potato roots infected by ceratocystis fimbriata. 196316655821
ceratocystis infection in sweet potato: its effect on proteins, isozymes, and acquired immunity.changes take place in the protein and isozyme patterns of tissue adjacent to cut surfaces of sweet potato roots infected by the fungus ceratocystis fimbriata. chromatography and gel electrophoresis of extracts from sections cut at known distances from a plane of infection showed that inoculation with a pathogenic or nonpathogenic isolate produced similar changes in several proteins and enzymes. inoculation of a susceptible variety of sweet potato with the nonpathogenic isolate induced in a thin ...196414199715
o-diphenol oxidases in sweet potato infected by the black rot fungus. 196514299590
effect of gossypol on some oxidative respiratory enzymes.gossypol was examined in relation to its effect on certain enzymes and enzyme complexes associated with the tricarboxylic acid cycle and the electron transport system. succinic dehydrogenase and cytochrome oxidase activity from sweet potato was completely inhibited by gossypol at 7.5 x 10(-3)m and 2.0 x 10(-3)m, respectively. succinoxidase activity of the same preparations was fully inhibited at a lower concentration, 2.5 x 10(-4)m. this concentration did not affect either succinic dehydrogenase ...19664287149
dna from plant mitochondria.dna was isolated from a mitochondrial fraction of each of the following plant materials: mung bean (phaseolus aureus) etiolated hypocotyl; turnip (brassica rapa) root; sweet potato (ipomoea batatas) root; and onion (allium cepa) bulb. it was found that all of these mitochondrial fractions contained dna, the densities of which were identical (rho=1.706 g.cm(-3)). an additional dna (rho=1.695) band found in the mitochondrial fraction of brassica rapa, was identical to dna separately isolated from ...196616656268
increase of mitochondrial fraction in sweet potato root tissue after wounding or infection with ceratocystis fimbriata.the acid-insoluble nitrogen content, lipid content, and cytochrome oxidase activity in the mitochondrial fraction are found to increase during incubation of slices of sweet potato (ipomoea batatas) root tissue. these increases appear to be related to an increase in the number of the mitochondrial particles. the increase in the mitochondrial fraction is not accompanied by an increase in cell number. the nitrogen content in the mitochondrial fraction increases prior to the changes in the activity ...196616656382
increased disease resistance and enzyme activity induced by ethylene and ethylene production of black rot infected sweet potato tissue.exposure of root tissue from a susceptible variety of sweet potato to low concentrations of ethylene induced a resistance to infection by ceratocystis fimbriata and an increase in the activity of peroxidase and polyphenoloxidase in the tissue. susceptible tissue that was inoculated with a pathogenic strain of c. fimbriata or a nonpathogenic strain that can induce resistance liberated more ethylene into closed chambers than tissue inoculated with strains that did not induce resistance. it is sugg ...196616656431
evaluation of several methods for analysis of sweet potato phenolics. 1967546955
invertase inhibitors from red beet, sugar beet, and sweet potato roots.invertase inhibitors have been isolated and partially purified from red beets, sugar beets, and sweet potatoes. these inhibitors are thermolabile proteins with molecular weights of 18,000 to 23,000. they do not inhibit yeast and neurospora invertases, but they are reactive with potato tuber invertase and other plant invertases with ph optima near 4.5. there are differences in reactivity of the inhibitors with some of the plant invertases, however. for most invertases, red beet and sugar beet inh ...196816656933
toxicity of mould-damaged sweet potatoes (ipomoea batatas). 19705428477
cytological evidence on the origin of sweet potato.the results of intensive meiotic studies, particularly of the karyology and chromosomal homology at the pachytene stage, in the sweet potato (ipomoea batatas l.), which is a hexaploid (2 n = 90), have thrown considerable light on its origin and genome relationships. using suitable criteria, such as relative length of chromosomes, centromere position, chromomere pattern, absence of light staining segments in one of the arms, presence of telochromomere etc., 40 of the 45 haploid chromosome complem ...197024435948
oxidative activity of mitochondria isolated from plant tissues sensitive and resistant to chilling injury.arrhenius plots of the respiration rates of mitochondria isolated from chilling sensitive plant tissues (tomato and cucumber fruit, and sweet potato roots) showed a linear decrease from 25 c to about 9 to 12 c (with q(10) values of 1.3 to 1.6), at which point there was a marked deviation with an increased slope as temperatures were reduced to 1.5 c (q(10) of 2.2 to 6.3). the log of the respiration rate of mitochondria from chilling resistant tissues (cauliflower buds, potato tubers, and beet roo ...19705427108
etiology of caries in papua-new guinea. associations in soil, food and water.mineral analyses of garden soils, vegetables, and water samples from 21 villages in the sepik and fly river regions of the territory of papua and new guinea, in which the mean prevalence of dental caries ranged from 0 to 29.5% decayed teeth per person, have shown the following relationships between variations in the caries prevalence and the amounts of certain elements:(1) a strong inverse association with concentrations of alkali and alkaline earth elements, especially strontium, barium, potass ...19705314012
de novo synthesis of peroxidase isozymes in sweet potato slices.the peroxidase content of sweet potato slices (ipomoea batatas lam.) increased nearly 100-fold following 84 hours incubation in an air atmosphere containing ethylene, 1 microliter per liter. the object of experiments reported here is to determine if this increase in peroxidase activity results from synthesis de novo of the enzyme or from activation of a preexisting inactive form of the enzyme.the enzymatic activity of each peroxidase isozyme increased during the incubation period, and each perox ...197116657648
the action of sweet potato -amylase on glycogen and amylopectin: formation of a novel limit dextrin. 19715165519
effect of triton x-100 on sweet potato -amylase. 19724622930
nature of sweet potato resistance to meloidogyne incognita and the effects of temperature on parasitism.penetration, rate of development, and total population of meloidogyne incognita in roots of susceptible 'allgold' and resistant 'nemagold' sweet potatoes increased with temperature 24-32 c. rate of larval penetration in 'allgold' was significantly higher than in 'nemagold' after 48 hr of root exposure at 24, 28, and 32 c. at 24, 28, and 32 c (16 hr) day and 20 c (8 hr) night temperature the life cycle of m. incognita required 42, 32, and 28 days in 'allgold', and 44, 33, and 31 days in 'nemagold ...197219319238
confirmation by chemical synthesis of the structure of 4-ipomeanol, a lung-toxic metabolite of the sweet potato, ipomoea batatas. 19724502825
distribution of nitrogen and composition of nitrogen compounds in food, urine and faeces in habitual consumers of sweet potato and taro. 19725068464
rooted leaves for physiological experiments.rooted leaves provide convenient material for investigating various physiological processes. details are given for producing rooted leaves of species with tuberous roots, e.g. sweet potato (ipomoea batatas) and dahlia (dahlia variabilis) and species with tap roots, e.g. sugar beet (beta vulgaris) and runner bean (phaseolus coccineus). the storage root formed on a single sugar-beet leaf develops concentric cambia suggesting that their formation is not determined by particular leaves.197224481792
studies on chlorogenic acid biosynthesis in sweet potato root tissue in special reference to the isolation of a chlorogenic acid intermediate.marked polyphenol production takes place in root tissue of sweet potato, ipomoea batatas lam. cv. norin 1, in response to slicing. a possible intermediate, tentatively termed compound v, of chlorogenic acid biosynthesis was isolated from the root tissue administrated with t-cinnamic acid-2-(14)c. compound v was proved to be an ester whose acid moiety was t-cinnamic acid, and the hydroxyl group-bearing moiety appeared to be a carbohydrate. compound v was suggested to be the first intermediate aft ...197316658406
comparative studies on tobacco pith and sweet potato root isoperoxidases in relation to injury, indoleacetic acid, and ethylene effects.sweet potato (ipomoea batatas) root parenchyma and tobacco (nicotiana tabacum) stem pith, both known to increase peroxidase activity after excision, differed from each other in their isoperoxidase patterns and in the isoperoxidase responses to injury and exogenous ethylene.in potato root sections, the injury-dependent peroxidase increase was due to an induction of two isoenzymes, as well as to a promotion of some constitutive ones. in tobacco pith, this increase was entirely due to seven isopero ...197316658496
cell wall and protoplast isoperoxidases in relation to injury, indoleacetic acid, and ethylene effects.in tobacco (nicotiana tabacum) pith, sweet potato (ipomoea batatas), and carrot (daucus carota) storage roots, differences were found between cell wall and protoplast peroxidases in their isoenzyme patterns, activity, and reaction to tissue injury.in the pith of elongating tobacco internodes, 90% of total activity was associated with the walls, 80% of which was due to the ionically and covalently bound fractions. with senescence, the increase in activity occurred mainly in the protoplast and the ...197416658744
dehydroipomeamarone as an intermediate in the biosynthesis of ipomeamarone, a phytoalexin from sweet potato root infected with ceratocystis fimbriata.recently, we isolated dehydroipomeamarone, a new sesquiterpenoid from sweet potato (ipomoea batatas lam.) root tissue infected with ceratocystis fimbriata (ell. et halst.). the purpose of this investigation was to determine whether dehydroipomeamarone was a precursor in the biosynthetic pathway of ipomeamarone. the incorporation of acetate-2-(14)c into ipomeamarone was markedly inhibited by the presence of dehydroipomeamarone. radioactive dehydroipomeamarone was efficiently converted into ipomea ...197416658760
transition of metabolisms in living popular bark from growing to wintering stages and vice versa: changes in glucose 6-phosphate and 6-phosphogluconate dehydrogenase activities and in the levels of sugar phosphates.activities of glucose 6-phosphate, 6-phosphogluconate, and isocitrate dehydrogenases, together with intermediate levels of the glycolytic pathway and the pentose phosphate cycle, were measured throughout a year in the living bark of poplar (populus gelrica). shoots, immediately after budding (early may), contained very high levels of the three enzyme activities, which fell gradually by early or mid-july to a level, roughly equivalent to budding (may) or growing (july) 2-year-old twigs. in septem ...197416658925
change in invertase activity of sweet potato in response to wounding and purification and properties of its invertases.when root tissue of sweet potato (ipomoea batatas lam.) was sliced, acid invertase activity, initially absent in freshly sliced tissue, appeared after a 3- to 6-hour lag phase, rapidly reached a maximum in 18 hours, and thereafter decreased. the increase in invertase activity was accompanied by a decrease in sucrose content of the root tissue. alkaline invertase activity was present in fresh root tissue, but changed little after wounding. acid invertase in wounded tissue and alkaline invertase i ...197416658839
lung-toxic furanoterpenoids produced by sweet potatoes (ipomoea batatas) following microbial infection. 19744373055
cyanide-resistant respiration of sweet potato mitochondria.the oxidation of malate and succinate by sweet potato mitochondria (ipomoea batatas [l.] lam.) was blocked only partly by inhibitors of complexes iii (2-heptyl-4-hydroxyquinoline-n-oxide) and iv (cyanide and azide). the respiration insensitive to inhibitors of complexes iii and iv was inhibited by salicylhydroxamic acid. essentially complete inhibition was obtained with inhibitors of complex i (rotenone, amytal, and thenoyltrifluoroacetone) and complex ii (thenoyltrifluoroacetone). the observati ...197516659084
evidence for covalently attached p-coumaric acid and ferulic acid in cutins and suberins.p-coumaric acid (4-hydroxycinnamic acid) and ferulic acid (4-hydroxy-3-methoxycinnamic acid) have been identified as constituents of cutin. their reduction products were isolated from a phenolic fraction released from the cutin of the fruits of apple, peach, pear, and two varieties of tomato and apple leaf by treatment with lialh(4) or liald(4). they were identified by combined gas chromatography and mass spectrometry. p-coumaric acid was present in all samples of cutin (0.07-0.53% by weight), w ...197516659364
determination of structure and composition of suberin from the roots of carrot, parsnip, rutabaga, turnip, red beet, and sweet potato by combined gas-liquid chromatography and mass spectrometry.suberin from the roots of carrots (daucus carota), parsnip (pastinaca sativa), rutabaga (brassica napobrassica), turnip (brassica rapa), red beet (beta vulgaris), and sweet potato (ipomoea batatas) was isolated by a combination of chemical and enzymatic techniques. finely powdered suberin was depolymerized with 14% bf(3) in methanol, and soluble monomers (20-50% of suberin) were fractionated into phenolic (<10%) and aliphatic (13-35%) fractions. the aliphatic fractions consisted mainly of omega- ...197516659124
toxigenic fungi in food.forty-five fungal isolates from moldy supermarket foods were tested for toxicity to brine shrimp, and twenty-two of these isolates were subsequently tested for toxicity to chicken embryos. highly toxigenic fungi were cladosporium sphaerospermum from a bakery product, fusarium oxysporum from carrots, f. solani from cabbage, aspergillus niger and penicillium corylophilum from bread, p. cyclopium and p. herguei from corn meal, p. lanosum from onions,p. steckii from chocolate syrup, penicillium sp. ...19751147614
immunochemical studies on fluctuation of phenylalanine ammonia-lyase activity in sweet potato in response to cut injury.antibody toward phenylalanine ammonia-lyase (pal) [ec 4.3.1.5] was obtained by immunization of a rabbit with highly purified pal. the antibody reacted specifically with ral, as demonstrated by the ouchterlony double diffusion test, immunoelectrophoresis, and sds polyacrylamide gel electrophoresis of the immunoprecipitate. experiments using anti-pal showed that pal was not present in fresh sweet potato tissue, but appeared in response to cut injury, reaching a maximum, and then decreasing, in par ...1976939758
cell isoperoxidases in sweet potato plants in relation to mechanical injury and ethylene.leaves and storage roots of sweet potato plants (ipomea batatas) showed the same qualitative isoperoxidase patterns and a similar distribution of distinctive isoperoxidases between the cell protoplast and cell wall free, ionically bound, and covalently bound fractions. no changes in the qualitative isoenzyme spectrum were found in relation to age, mechanical injury, or ethylene action. thus, as in tobacco plants, the cell isoperoxidases in sweet potato did not reflect the possible differential m ...197616659429
induction of furano-terpene production and formation of the enzyme system from mevalonate to isopentenyl pyrophosphate in sweet potato root tissue injured by ceratocystis fimbriata and by toxic chemicals.when sweet potato (ipomoea batatas) root tissue was infected by ceratocystis fimbriata, activity of the enzyme system from mevalonate to isopentenyl pyrophosphate, especially of pyrophosphomevalonate decarboxylase (ec 4.1.1.33), was increased in the noninfected tissue adjacent to the infected region, preceding the furano-terpene production in the infected region. cutting and incubation of sweet potato slices did not produce furano-terpenes, and only slightly increased the activity of the enzyme ...197616659619
[the effect of phospholipids on starch metabolism].the presence of phospholipids reduces the breakdown of amylose catalyzed by β-amylase, phosphorylase and α-amylase. the activities of the β-amylases of sweet potato (ipomoea batatas) and barley (hordeum vulgare l.) disminish to less than 10% of the activity in the control without the phospholipids. when the amylose was complexed with phospholipids the activity of the α-amylase of bacillus subtilis was reduced to about 25% of the control value. a similar effect was observed for the amylases of ze ...197624430907
host plants, distribution, and ecological association of hoplolaimus columbus.selected species and cultivars of plants were evaluated for host suitability for hoplolaimus columbus under greenhouse and fieht conditions. sixteen agronomic plant cultivars were assayed for infection and reproduction after 2-6 months. lima bean, soybean, cotton, and sweet corn were most favorable for nematode reproduction, whereas sweet potato was a nonhost plant. field corn and watermelon, which tolerated h. columbus without significant yield losses, are suited for alternative crops in the so ...197619308233
trypsin-induced atpase activity in potato mitochondria.potato mitochondria (solanum tuberosum var. russet burbank), which readily phosphorylate adp in oxidative phosphorylation, show low levels of atpase activity which is stimulated neither by mg(2+), 2,4-dinitrophenol, incubation with respiratory substrates, nor disruption by sonication or treatment with triton x-100, individually or in concert. treatment of disrupted potato mitochondria with trypsin stimulates mg(2+)-dependent, oligomycin-sensitive atpase activity 10- to 15-fold, suggesting the pr ...197616659532
purification and properties of sweet potato mild mottle, a white-fly borne virus from sweet potato (ipomoea batatas) in east africa. 19761267233
polarity of production of polyphenols and development of various enzyme activities in cut-injured sweet potato root tissue.investigation of polyphenol production in cut-injured sweet potato (ipomoea batatas lam. cv. kokei 14) roots by histochemical and quantitative methods showed that polyphenols were produced in striking amounts in the proximal side of the tissue pieces (2 cm thick), but only in small amounts in cells of the distal side. in response to cut injury, formation of the enzymes related to polyphenol biosynthesis, phenylalanine ammonia-lyase and trans-cinnamic acid 4-hydroxylase, was also pronounced in th ...197716660137
synthesis and apparent turnover of acid invertase in relation to invertase inhibitor in wounded sweet potato root tissue.previously we showed that acid invertase activity increased and then decreased rapidly in wounded sweet potato (ipomoea batatas liam.) root tissue, and that the tissue contained a heat-stable, proteinaceous inhibitor with a molecular weight of about 19,500 daltons.in response to wounding of sweet potato root tissue, inhibitor activity decreased during the increase in invertase activity but later increased slightly when invertase activity declined. cycloheximide treatment did not affect the decre ...197716659960
biogenesis of lung-toxic furans produced during microbial infection of sweet potatoes (ipomoea batatas). 1977864134
synthesis and turnover of phenylalanine ammonia-lyase in root tissue of sweet potatoe injured by cutting.1. antibody toward phenylalanine ammonia-lyase of root tissue of sweet potato injured by cutting was obtained by immunizing of a rabbit with purified enzyme. 2. phenylalanine ammonia-lyase activity developed markedly in disks of sweet potato roots in response to cut injury, reached a maximum after 12 h, and then decreased thereafter. phenylalanine ammonia-lyase content, which was determined by the quantitative immunoprecipitation method, changed in parallel with enzyme activity. the rate of inco ...1977837940
bacterial stalk rot of maize, its symptoms and host-range.stalk rot of maize, caused by erwinia carotovora f. sp. zeae sabet (re-designated as pectobacterium chrysanthemi pathovar. zeae by kelman 1974) showed first premature withering and drying up of the uppermost leaves which was soon followed by the lower leaves. the rot either extended from the base upwards (basal rot) or from the top downwards (top rot). in the case of basal rot, the leaves become yellow and the infected tissue becomes brown, soft, and water soaked. internally, the stalk turns int ...1977857508
pig-bel--a zoonosis?it is postulated that the combination of a very low protein diet, with low pancreatic tryptic activity, plus the continual consumption of sweet potato trypsin inhibitors leads to inadequate proteolysis when a meal containing meat contaminated with c. perfringens type c and its beta toxin is consumed. the necrotizing pathogenic process concluding as segmental gangrene is set in motion by toxin produced under altered dietary conditions first by motor paralysis via beta toxin, followed by villous a ...1978731766
isolation and characterization of factors in sweet potato root which agglutinate germinated spores of ceratocystis fimbriata, black rot fungus.a factor which agglutinates the germinated spores of ceratocystis fimbriata was isolated from the sweet potato root. the factor is a glycoprotein with a molecular weight of 1.6 x 10(6) daltons and required divalent cations such as ca(2+), mn(2+), ni(2+), and mg(2+) for activity. the activity of the factor was ph-dependent. the factor also agglutinated rabbit erythrocytes and is classified as a phytohemagglutinin or lectin. the factor agglutinated germinated spores of seven strains of c. fimbriat ...197816660599
cyanide-resistant respiration in fresh and aged sweet potato slices.the respiration of fresh sweet potato (ipomoea batatas) slices is resistant to, and often stimulated by, cyanide and antimycin a. m-chlorobenzhydroxamic acid (clam), a selective inhibitor of the alternate path, inhibits respiration in the presence of cyanide and has a limited inhibitory effect in the presence of antimycin a. thus, a partial bypass of the antimycinsensitive site is indicated. respiration rises 2-fold at best with slice aging, the increment being cytochrome-mediated. the cyanide-r ...197816660494
solubilization of udp-glucose-specific starch synthetase in sweet-potato (ipomoea batatas) starch granules by the urea/pullulanase method.the adp-glucose- or udp-glucose-specific starch synthetase bound to sweet-potato (ipomoea batatas) starch granules is localized in the granules, and the udp-glucose-specific enzyme was solubulized by urea/pullulanase treatment of the starch granules.19806450591
composition of suberin-associated waxes from the subterranean storage organs of seven plants : parsnip, carrot, rutabaga, turnip, red beet, sweet potato and potato.the waxes associated with the suberin in the periderm of the underground storage organs of parsnip (pastinaca sativa l.), carrot (daucus carota l.), rutabaga (brassica napobrassica mill.), turnip (brassica rapa l.), red beet (beta vulgaris l.), sweet potato (ipomoea batatas l.) and potato (solanum tuberosum l.) were isolated, fractionated into hydrocarbon, wax ester, free fatty alcohol and free fatty acid fractions, and analyzed by combined gas chromatography and mass spectrometry. the amount of ...198024310190
melanesian prehistory: some recent advances.human occupation of new guinea had begun 50,000 years ago, but islands further east were settled only in recent times. in part of the new guinea highlands, wet and dry horticultural systems began by 9000 years ago. local intensification is evident until the present, but only the most recent major crop (sweet potato, which has been grown in the region for less than 300 years) is documented. on the south coast, exchange systems and economies locally diversify over the last two millennia. in the me ...198017795991
inhibition of electron transport and oxidative phosphorylation in plant mitochondria by gladiolic acid and structurally-related aromatic ortho dialdehydes.gladiolic acid (ga, 4-methoxy-5-methyl-0-phthalaldehyde-3-carboxylic acid), an antifungal aromatic ortho dialdehyde produced by penicillium gladioli was found to be a potent inhibitor of electron transport and oxidative phosphorylation reactions in sweet potato and mung bean mitochondria. similar results were also found with the naturally occurring ortho dialdehydes, cyclopaldic acid, quadrilineatin, and flavipin as well as the synthetic dialdehydes, 3-formyl opianic acid and 0-phthalaldehyde. b ...19806248181
experimental pigbel: the production and pathology of necrotizing enteritis due to clostridium welchii type c in the guinea-pig.an animal model for pigbel in man was developed using guinea-pigs. intragastric dosing with growing cultures of clostridium welchii type c only produced necrotic lesions if protease inhibitors were given as well. beta toxin, which is made by the type c organism, causes the intestinal damage and is very easily destroyed by proteases. protease inhibitors in soybean and aprotinin were effectively in inducing disease in animals on a normal diet, while inhibitors in sweet potato, which inhibit only t ...19806252934
biological quality and composition of sweet potato protein fragments. 19817276386
studies on a factor in sweet potato root which agglutinates spores of ceratocystis fimbriata, black rot fungus.a factor which agglutinated the spores of ceratocystis fimbriata in the presence of ca(2+) was purified from sweet potato (ipomea batatas lam cv. norin[1]) root. element composition of the purified factor was as follows; analysis found: c (29.8%), h (3.97%), o (65.34%), n (0.81%): calculated for c(43)h(69)o(70)n(1): c (30.02%), h (4.01%), o (65.15%), n (0.81%). the factor was mainly composed of galacturonic acid (53% of dry weight) and contained arabinose, fucose, and unidentified component as m ...198216662232
properties of a mixed function oxygenase catalyzing ipomeamarone 15-hydroxylation in microsomes from cut-injured and ceratocystis fimbriata-infected sweet potato root tissues.ipomeamarone 15-hydroxylase activity was found in a microsomal fraction from cut-injured and ceratocystis fimbriata-infected sweet potato (ipomoea batatas lam. cv. norin no. 1) root tissues and its optimum ph was 8.0. the enzyme reaction required o(2) and nadph. the k(m) values calculated for ipomeamarone and nadh were approximately 60 and 2 micromolar, respectively. nadph alone had little effect on enzyme activity but activated the reaction in the presence of low concentrations of nadph. ipomea ...198216662536
inhibition of photosynthesis by ethylene-a stomatal effect.ethylene at hormonally significant levels inhibited net photosynthesis of the cultivated peanut (arachis hypogaea l.) as measured by gas analysis. upon the removal of ethylene, the inhibition was naturally overcome at the concentration-exposure duration combinations tested. increased length of exposure of 1 microliter of ethylene per liter of air up to 6 hours increased the degree of net photosynthesis inhibition (68% reduction after 6-hour exposure). significantly greater inhibition of photosyn ...198216662540
malate oxidation, rotenone-resistance, and alternative path activity in plant mitochondria.the effect of cyanide and rotenone on malate (ph 6.8), malate plus glutamate (ph 7.8), citrate, alpha-ketoglutarate, and succinate oxidation by cauliflower (brassica oleracea l.) bud, sweet potato (ipomoea batatis l.) tuber, and spinach (spinacia oleracea and kalanchoë daigremontiana leaf mitochondria was investigated. cyanide inhibited all substrates equally with the exception of malate plus glutamate; in this case, inhibition of o(2) uptake was more severe due to an effect of cyanide on aspart ...198216662651
carbon dioxide effects on ethanol production, pyruvate decarboxylase, and alcohol dehydrogenase activities in anaerobic sweet potato roots.the effect of varied anaerobic atmospheres on the metabolism of sweet potato (ipomoea batatas [l.] lam.) roots was studied. the internal gas atmospheres of storage roots changed rapidly when the roots were submerged under water. o(2) and n(2) gases disappeared quickly and were replaced by co(2). there were no appreciable differences in gas composition among the four cultivars that were studied. under different anaerobic conditions, ethanol concentration in the roots was highest in a co(2) enviro ...198316662798
protein nutritional value of sweet potato flour. 19836415140
utilization of fodder yeast and agro-industrial by-products in production of spores and biologically - active endotoxins from bacillus thuringiensis.a number of newly-devised fermentation media were evaluated with respect to their ability to support sporulation and biosynthesis of endotoxins by strains of bacillus thuringiensis that are biologically active against spodoptera littoralis, heliothis armigera, and spodoptera exigua. fodder yeast from dried cells of saccharomyces cerevisiae could be used as a complete mono-component medium for production of highly active spore-delta-endotoxin complexes from b. thur., vars. entomocidus, kurstaki a ...19836666415
production of oxalic acid by some fungi infected tubers.oxalic acid (as oxalate) was detected in four tubers commonly used for food in nigeria-dioscorea rotundata (white yam), solanum tuberosum (irish potato), ipomoea batatas (sweet potato), and manihot esculenta (cassava). whereas healthy i. batata had the highest oxalic acid content, healthy m. esculenta contained the lowest. when all tubers were artifically inoculated with four fungi-penicillium oxalicum curie and thom, aspergillus niger van tiegh, a. flavus and a. tamarii kita, there was an incre ...19836670294
effect and reproduction of rotylenchulus reniformis on sweet potato selections.growth, yield, and quality of 10 sweet potato selections and reproduction of the reniform nematode, rotylenchulus reniformis, were studied in fumigated and nonfumigated plots in a naturally infested field. nematode reproduction on the different selections in the field was similar to that reported in the greenhouse but was not related to the effect of the nematode on yield of the different selections. goldrush supported the least reproduction but was the most severely affected by the nematode, wh ...198319295791
population dynamics of meloidogyne incognita and rotylenchulus reniformis alone and in combination, and their effects on sweet potato.meloidogyne incognita (mi) and rotylenchulus reniformis (rr) interactions on sweet potato were studied in naturally and artificially infested field plots for 3 years. in a naturally infested field, early season counts of mi or rr were positively correlated with later counts of the same nematode, but negative correlations were found between early mi and subsequent rr, and early rr and subsequent mi counts. in field plots fumigated with methyl bromide and then infested with low levels of rr, mi, a ...198319295792
effects of concomitant development on reproduction of meloidogyne incognita and rotylenchulus reniformis on sweet potato.the influence of various factors on reproduction of concomitant meloidogyne incognita (mi) and rotylenchulus reniformis (rr) on sweet potato were studied in the greenhouse. reproduction of rr was reduced by mi at all inoculum levels and experiment durations used, while mi reproduction was not inhibited. both species failed to affect each other when inoculated simultaneously onto root systems developed in separate pots from different nodes of the same plant. reproduction of each species was not s ...198319295794
cholesterol binding capacity of fiber from tropical fruits and vegetables.the cholesterol binding capacity of 28 fiber samples from a variety of the more common tropical fruits and vegetables was determined. the binding capacity of cholestyramine, cellulose, lignin, guar gum and citrus pectin were also determined. capacities were evaluated by an in vitro method that simulates the effect of the human digestive system on fiber using a series of enzymatic treatments before the binding was determined. binding values varied from 3% for a soluble fraction of cassava to 84% ...19846323908
isolation of furanoterpene-containing particles from ceratocystis fimbriata-infected sweet potato root tissue.furanoterpene-containing particles were isolated by centrifugation on a discontinuous ficoll density gradient from a homogenate of the non-infected tissue adjacent to the infected region of ceratocystis fimbriata-infected sweet potato root tissue. the particles were recovered at a relatively high ratio in the 2% ficoll fraction, in which there was no contamination by mitochondria and only little by endoplasmic reticulum judging from the distribution of the activities of their marker enzymes and ...19846099359
partial purification and characterization of udpg:t-cinnamate glucosyltransferase in the root of sweet potato, ipomoea batatas lam.previously, we isolated t-cinnamoyl-d-glucose as a possible intermediate in chlorogenic acid biosynthesis from sweet potato root. the enzyme which catalyzes the formation of t-cinnamoyl-d-glucose has been purified 539-fold from sweet potato root (ipomoea batatas lam.) and characterized. it required udp-glucose as a glucosyl donor. its molecular weight was estimated to be 45,000 by gel filtration chromatography through sephadex g-100. its km values were 0.2 mm for t-cinnamic acid and 0.1 mm for u ...19846231280
intracellular sites of the synthesis of sweet potato mitochondrial f1atpase subunits.five subunits (α-, β-, γ-, δ- and δ'-subunits) of the six α∼δ'-and ε-subunits) in the f1 portion (f1atpase) of sweet potato (ipomoea batatas) mitochondrial adenosine triphosphatase were isolated by an electrophoretic method. the δ- and δ'-subunits were not distinguishable immunologically but showed completely different tryptic peptide maps, indicating that they were different molecular species. in vitro protein synthesis with isolated sweet potato root mitochondria produced only the α-subunit wh ...198524306987
occurrence of anthocyanoplasts in cell suspension cultures of sweet potato.intensely pigmented and spherical vesicles (anthocyanoplasts) were found in anthocyanin-containing cells of sweet potato (ipomoea batatas) suspension cultures. anthocyanin synthesis began to first occur 24-48 h after exposure to light, and then numerous small red vesicles were detected under a microscope. the frequency of anthocyanoplast-containing cells rapidly increased to finally about 80% of the total cultured cells after 5 days of irradiation. fully developed anthocyanoplasts reached 10-15 ...198524253981
isolation and culture of protoplasts from high anthocyanin-producing callus of sweet potato.optimum conditions for the isolation and culture of protoplasts from high anthocyanin-producing callus of sweet potato (ipomoea batatas) were investigated. growth phase of callus and the ratio of callus-enzyme solution affected the yield of protoplasts. composition of the medium and protoplast density were examined for protoplast culture.small colonies were regenerated from the protoplasts. upon transfer to light a high amount of anthocyanin was accumulated in these colonies.198524253980
nutrition and burrowing energetics of the cape mole-rat georychus capensis.at 22°c the resting oxygen consumption of g. capensis is 1.13±0.05 cm(3)o2·g(-1)·h(-1) (mean± s.e.). in loose sandy soil the burrowing metabolic rate was approximately three times that of resting (3.41±0.19 cm(3)o2·g(-1)· h(-1)). rate of oxygen consumption while burrowing bears a linear relationship with rate of burrowing. the equation of the regression line describing this relationship was used to construct a model for calculating energy expenditure of burrowing in free-living mole-rats. the di ...198528310816
effects of commonly consumed vegetables on hepatic xenobiotic-metabolizing enzymes in the mouse.to investigate the effects of consuming a variety of common vegetables on the activities of xenobiotic-metabolizing enzymes in the mouse liver, male c57bl/6 mice were fed purified diets supplemented isocalorically with 20% freeze-dried powdered kidney bean, soya bean, alfalfa, cauliflower, mustard greens, carrot, kale, brussels sprouts, beet, egg plant or onion or with 40% sweet potato. hepatic ethoxycoumarin o-deethylase (ecd) activity was increased significantly (p less than or equal to 0.05) ...19853877667
the nephrotoxicity for mice of deisopropylngaione, a minor furanoid component of toxic myoporaceous essential oils.deisopropylngaione (din) is one of a family of hepatotoxic furanosesquiterpenoid essential oils which is found in small amounts (5%) in the leaves of some specimens of the australian plant myoporum deserti. din differs from other furanoid myoporaceous essential oils in that it also causes lesions in the lungs and kidneys. at the near ld50 dose rate of 150 mg kg-1 given by intraperitoneal injection, din is able to cause lethal renal proximal tubular necrosis without causing significant injury to ...19853989217
monoclonal antibodies against the aster yellows agent.hybridoma clones secreting specific monoclonal antibodies against the aster yellows agent, a mycoplasma-like organism, were produced by using partially purified salivary gland preparations from infected leafhopper vectors as the immunogen. after 3947 hybridomas from 20 independent fusions were screened for specific antibody against the aster yellows agent, two table clones were obtained. with these monoclonal antibodies the aster yellows agent in diseased lettuce, periwinkles, and inoculative in ...198517757867
starch phosphorylase inhibitor from sweet potato.a protein, starch phosphorylase inhibitor, was purified from the root of sweet potato (ipomoea batatas [l.] lam. cv tainon 65). it had a molecular weight of 250,000 and could be composed of five identical subunits. the isoelectric point of the inhibitor was 4.63. it was a noncompetitive inhibitor toward the sweet potato enzyme with a k(i) value of 1.3 x 10(-6) molar when glucose-1-p was the variable substrate. because cross-reacting materials of rabbit antiphosphorylase inhibitor of sweet potato ...198616664657
phase transitions in liposomes formed from the polar lipids of mitochondria from chilling-sensitive plants.the thermal response of mitochondrial polar lipids from a variety of chilling-sensitive and chilling-insensitive plants was determined by differential scanning calorimetry. a phase transition was observed at 15 degrees c for mitochondria from soybeam (glycine max. cv davis) hypocotyl, at 16 degrees c for tomato (lycopersicon esculentum cv flora-dade and cv grosse lisse) fruit, at 15 degrees c for cucumber (cucumus sativus l.) fruit, at 14 degrees c for mung bean (vigna radiata var berken) hypoco ...198616664907
protease production with sweet potato residue by solid state fermentation.the sweet potato residue being at the initial moisture content 50-58%, initial ph 3.5-4.3, supplemented with rice bran, and minerals, and incubated at 20-26 degrees c for 5 days was the optimal conditions for protease production with aspergillus niger ntu-am-1 by solid state fermentation. protease could be recovered by shaking at room temperature for one hour and extracted with five times volume of 0.1% nacl solution. the yield of protease was 814 units per gram dry weight of substrate. partiall ...19863545704
influence of meloidogyne incognita on resistant and susceptible sweet potato cultivars.effects of several population densities ofmeloidogyne incognita on the sweet potato cultivars centennial (susceptible) and jasper (moderately resistant) were studied. field plots were infested with initial levels (pi) of 0, 10, 100, 1,000, 5,000, and 10,000 eggs and juveniles/500 cm(3) soil in 1980 and 0, 100, 1,000, 2,000, 3,000, 4,000, and 5,000 in 1981. m. incognita population development trends were similar on both cultivars; however, at high pi, more eggs and juveniles were recovered from c ...198619294141
purification and characterization of hydroxycinnamoyl d-glucose. quinate hydroxycinnamoyl transferase in the root of sweet potato, ipomoea batatas lam.we have previously proposed a chlorogenic acid biosynthetic pathway which involves a transesterification reaction between hydroxycinnamoyl d-glucose and d-quinic acid. the proposed pathway was based on tracer experimental results (kojima, m., and uritani, i. (1972) plant cell physiol. 13, 311-319). the enzyme that catalyzes the above reaction has been purified 160-fold from sweet potato root (ipomoea batatas lam.) and characterized. the purified enzyme yielded one band of 26,000 daltons on sodiu ...19863722170
heritability of regeneration in tissue cultures of sweet potato (ipomoea batatas l.).a population of open-pollinated progeny from 12 parents, and the 12 parents, was surveyed for in vitro growth and regeneration characteristics. four different tissue culture procedures involving different media and the use of different explants to initiate the cultures were used. petiole explants from young leaves were used as explants for initiation of callus cultures. these were evaluated for callus growth rate, friability, and callus color and texture, before transferring to each of three dif ...198624247711
plant regeneration from protoplast culture of sweet potato (ipomoea batatas lam.).this is the first report on successful plant regeneration from protoplasts of sweet potato. two cultivars (guyana and duclos xi) of sweet potato plants propagated under in vitro conditions were used as the source of protoplasts. green compact calli with meristematic areas were induced in the medium supplemented with 2mg1(-1) zeatin, and plant regeneration occurred when these calli were transferred onto the medium with zeatin level reduced to 0.25mg1(-1). plant regeneration was found to be genoty ...198724248836
molecular cloning and nucleotide sequence of full-length cdna for sweet potato catalase mrna.a nearly full-length cdna clone for catalase (pcas01) was obtained through immunological screening of cdna expression library constructed from size-fractionated poly(a)-rich rna of wounded sweet potato tuberous roots by escherichia coli expression vector-primed cdna synthesis. two additional catalase cdna clones (pcas10 and pcas13), which contained cdna inserts slightly longer than that of pcas01 at their 5'-termini, were identified by colony hybridization of another cdna library. those three ca ...19872885193
processing of a plant vacuolar protein precursor in vitro.a precursor for sporamin a, the storage protein of the tuberous roots of sweet potato deposited in the vacuole, is synthesized on membrane-bound polysomes and has an extra peptide of 37 amino acids at the n-terminus of the mature form, which can be divided into an n-terminal putative signal peptide sequence (residues -37 to -17) and a segment enriched with charged amino acids (residues -16 to -1) [hattori, t., et al. (1985) plant mol. biol. 5, 313-320]. we examined the in vitro processing of the ...19873301345
quality improvement of sweet-potato (ipomoea batatas l. lam.) roots as feed by ensilage.1. sweet-potato (ipomoea batatas l. lam.) strips (sps) mixed with maize powder (cp) in proportions 10:0, 9:1, 8:2, and 7:3 were ensiled for 1, 2 or 3 months. 2. trypsin inhibitor activity (tia) decreased during ensilage in samples of all treatments while the sps-cp mixture (7:3, w/w) ensiled for 3 months contained the lowest tia. 3. sps-cp (8:2, w/w) dried or ensiled for 2 months, or ensiled for 2 months and dried, were each mixed with twice the amount of control diet (1:2, w/w) to make three di ...19883408700
characterization of catalase transcripts and their differential expression in maize.in maize, the three unlinked catalase (ec 1.11.1.6) structural genes (cat1, cat2 and cat3) are differentially expressed temporally, spatially and in response to environmental signals in the developing seedling. in order to understand more fully the molecular mechanisms involved in catalase gene expression, full-length cdna clones representing the maize cat1, cat2 and cat3 transcripts were isolated and characterized. dna sequence analysis confirmed that each cdna encodes a unique catalase protein ...19882461221
plant-parasitic nematode problems in the pacific islands.the pacific islands have a diverse range of food and cash crops with indigenous and introduced nematode problems. the staple food crops have serious nematode pests, such as meloidogyne spp. on sweet potato, hirschmanniella miticausa causing corm rot of taro, and pratylenchus coffeae and radopholus sp. producing tuber dry rot of yams. bananas are infested with p. coffeae or r. similis, citrus with tylenchulus semipenetrans, rice with aphelenchoides besseyi, and ginger with meloidogyne spp. and r. ...198819290200
nematode population densities and yield of sweet potato and onion as affected by nematicides and time of application.nematode population densities and yield of sweet potato and onion as affected by nematicides and time of application were determined in a 3-year test. population densities of meloidogyne incognita race 1 in untreated plots of sweet potato increased each year, but helicotylenchus dihystera and criconemella ornata did not. ethoprop (6.8 kg a.i./ha) incorporated broadcast in the top 15-cm soil layer each spring before planting sweet potato reduced population densities of nematodes in the soil and i ...198819290295
starch phosphorylase inhibitor is beta-amylase.the proteinaceous noncompetitive inhibitor of starch phosphorylase isolated from the root of sweet potato (ipomoea batatas [l.] lam.) (tc chang, jc su 1986 plant physiol 80: 534-538) has been identified as a beta-amylase. the starch phosphorylase inhibitor and beta-amylase activities copurified to give a protein indistinguishable from commercial beta-amylase by electrophoretic and immunological methods, and the two activities showed parallel responses in ph, temperature, and inhibitor sensitivit ...198816666436
analyzing the effects of exogeneous polyamines and growth regulators on plating efficiency of sweet potato protoplasts using a central composite test design.the effects of exogenous polyamines and growth regulators on plating efficiency of greenhouse-grown sweet potato (ipomoea batatas lam.) petiole protoplasts after six days were analyzed using a central composite test design. the medium components screened were 1-naphthaleneacetic acid (naa), 6-benzylaminopurine (bap), putrescine (put), spermidine (spd), and spermine (spm), each at five concentrations. stepwise multiple regression analysis revealed significant interaction of naa with bap, put, and ...198824241604
production of vesicular-arbuscular mycorrhizal fungus inoculum in aeroponic culture.bahia grass (paspalum notatum) and industrial sweet potato (ipomoea batatas) colonized by glomus deserticola, g. etunicatum, and g. intraradices were grown in aeroponic cultures. after 12 to 14 weeks, all roots were colonized by the inoculated vesicular-arbuscular mycorrhizal fungi. abundant vesicles and arbuscules formed in the roots, and profuse sporulation was detected intra-and extraradically. within each fungal species, industrial sweet potato contained significantly more roots and spores p ...198816347548
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