Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| advances in the research of celery, an important apiaceae vegetable crop. | celery (apium graveolens l.), one of the most important vegetables in apiaceae family, is cultivated worldwide and utilized in food and cosmetic industries because it is an excellent source of vitamins, phenolic compounds, volatile oils and other nutrients. celery extracts possess various medicinal properties, such as antibacterial, anti-inflammatory and lowering blood glucose and serum lipid levels. with the rapid advancements in molecular biology and sequencing technology, studies on celery ha ... | 0 | 28423952 |
| [factors affecting embryogenesis in tissue cultures from domestic forms of umbellifera]. | the cultivation of tissues from domestic forms of umbellifera (daucus carota, apium-graveolens) on simple, synthetic media resulted in the production of adventive embryos if the salts and trace elements of white's medium were replaced by those of the formula of murashige and skoog. in contrast to results with embryos from different seeds the positive effect of these components on the production and the early development of the adventive embryos in the tissue cultures obviously was not dependent ... | 1966 | 24557788 |
| some central effects of an essential oil of apium graveolens (linn.). | 1967 | 5594384 | |
| microfibril orientation in plant cell walls. | the distribution of particles on the surface of the plasmalemma in the collenchyma of apium graveolens was studied by the freeze-etching technique. the aim was to determine whether the distribution of particles was related to the known longitudinal or transverse orientation of cellulose microfibrils in different layers of the walls of these cells. preliminary statistical studies have shown no obvious correlation between particle distribution and microfibril orientation although the distribution ... | 1970 | 24500125 |
| some ideas for further research in reproduction. | comments are made on reproductive research problems in the hope that they may serve as a stimulus for effective research in the areas concerned. in studying the effects of drugs on the nerve elements involved in reproductive processes, no one has attempted to delineate the neural part of endometrial function, or to show if, or how it relates to the hormonal effect on the same tissues. hysterectomy, removal of about 75% of the uterus, and the denervation of the guinea pig uterus greatly extend ... | 1970 | 12254863 |
| a study of central pharmacological activity of alkaloid fraction of apium graveolens linn. | 1970 | 5439955 | |
| antifertility screening of plants. vi. effect of five indigenous plants on early pregnancy in albino rats. | the petroleum ether, alcoholic and aqueous extracts of apium graveolens linn., butea monosperma lam. kuntz., and gossypium herbaceum linn., the aqueous extract of aloe barbadensis mill.syn., and the juice of unripe fruits of ananas comosus were tested on albino rats by a method which detects any antizygotic, blastocystotoxic, antiimplantation, and early abortifacient activity. the extracts were administered for 1-7 days. the dosages for a. graveolens, b. monosperma, and g. herbaceum were 100 ... | 1970 | 5505214 |
| [volatile oil from celery leaves (apium graveolens l.)]. | 1974 | 4850124 | |
| [the effect of the larva's host plant (apium graveolens l.) on the egg-laying and the ovarian production of philophylla heraclei l. (dipters, terphritidae)]. | celery leaves--hostplant for larvae--have no trophic importance for adults and are not a site of rendez-vous. but their presence, even for four hours a day, strongly stimulates ovarian production, independently from egg-laying which varies with leaves age. | 1975 | 813867 |
| the distribution of p-protein in mature sieve elements of celery. | the extent of blocking of sieve-plate pores caused by release of cell turgor was investigated by fixing and processing for electron microscopy a long length of celery (apium graveolens l.) phloem. differences in distribution of p-protein within the pores were observed between those cells near the two cut ends, and the central cells.to assess the effect of chemical fixation on the distribution of p-protein, strands of celery phloem (fixed or unfixed, and not treated with cryoprotectants) were fro ... | 1975 | 24430087 |
| observations with cytochemistry and ultracryotomy on the fine structure of the expanding walls in actively elongating plant cells. | ultracryotomy with negative staining and cytochemistry (periodic acid - thiocarbohydrazide - silver proteinate test for polysaccharides, in conjunction with mild extractions) were used to study the architecture of the cell wall and its modifications during expansion. those techniques were applied to the study in situ of the walls of actively elongating parenchyma of mung bean (phaseolus aureus), and pea (pisum sativum) root and of collenchyma of celery (apium graveolens) petioles. these compleme ... | 1975 | 1202041 |
| investigations of the content of furocoumarins in apium graveolens and in petroselinum sativum. | 1976 | 948516 | |
| gibberellin-induced separation of cells in isolated endosperm of celery seed. | the mature seed of celery (apium graveolens, l.) contains a small axile linear embryo surrounded by endosperm which occpies the bulk of the seed. the endosperm is living and consists of mostly large angular thick-walled cells containing aleurone grains (often with globoids) and lipid droplets. - using de-embryonated seeds, it has been shown that the endosperm was induced to break down by gibberellin. the aleurone grains became swollen and lost their contents and the bulk of each cell wall was hy ... | 1976 | 24430902 |
| a freeze-etching and replication study of wall deposition in elongating plant cells. | the architecture of the expanding wall of mung bean hypocotyl (phaseolus aureus) and collenchyma of celery (apium graveolens) was examined using freeze-etching without any cryoprotectant, and surface-replication of frozen-ground and air-dried specimens. the polylamellated organization of the wall was seen. freeze-etching clearly visualized, within one single fracture plans, the intermediate strata in which the microfibril orientation gradually changes between the main transverse and longitudinal ... | 1978 | 753595 |
| the boar-pheromone steroid identified in vegetables. | the steroid 5 a-androst-16-en-3-one, known as a boar pheromone, was identified in parsnip (pastinaca sativa) and celery (apium graveolens). concentrations are in the range of 8 ng/g plant. | 1979 | 520500 |
| effect of koen--chai or chinese celery (apium graveolens) on spermatogenesis. | 1979 | 221607 | |
| a structural study of germination in celery (apium graveolens l.) seed with emphasis on endosperm breakdown. | germination of celery seed occurred after 6 d of imbibition in light. during this time the embryo enlarged at the expense of the adjacent endosperm cells and at the time of germination was 2-3 times as long as in the dry seed. breakdown of the endosperm cells near the root cap preceeded radicle emergence. none of these changes occurred in darkness.endosperm digestion began adjacent to the embryo and spread radially. in degrading cells, the aleurone grains often became larger and fewer in number. ... | 1979 | 24407254 |
| phosphatidylinositol phosphodiesterase in higher plants. | 1. the lower regions of the stem of celery (apium graveolens l.) contain a soluble enzyme that hydrolyses phosphatidylinositol. 2. the lipoidal product of hydrolysis is diacylglycerol, and the water-soluble products are 1:2-cyclic phosphoinositol and phosphoinositol in the approximate proportions of 60% and 40% respectively: this indicates that a phosphodiesterase (phospholipase c-like) activity is cleaving the phosphatidylinositol. 3. the enzyme requires a bivalent cation, ca2+ being the most e ... | 1980 | 6272700 |
| a new dihydrofurocoumarin from apium graveolens. | 1981 | 17402052 | |
| hplc analysis of linear furocoumarins (psoralens) in healthy celery (apium graveolens). | four linear furocoumarins (psoralen, bergapten, xanthotoxin, and isopimpinellin) were isolated from three varieties of healthy, commercially grown celery (apium graveolens). psoralen has not previously been reported to occur in celery. combined levels of these photomutagenic and photocarcinogenic furocoumarins measured by normal-phase hplc did not exceed 1.3 ppm in any of the celery varieties studied. | 1983 | 6682079 |
| a pathway for photosynthetic carbon flow to mannitol in celery leaves : activity and localization of key enzymes. | in the polyol producing plant, celery (apium graveolens l.), mannitol is a major photosynthetic product and a form in which carbohydrate is translocated. measurements of whole leaf extracts of celery indicated substantial activity of the following enzymes: mannose-6-p reductase, mannose-6-p isomerase, mannitol-1-p phosphatase, and nonreversible glyceraldehyde-3-p dehydrogenase. the activities of these enzymes were either undetectable or very low in the nonpolyol producing plants, secale cereale ... | 1983 | 16663332 |
| plant triose phosphate isomerase isozymes : purification, immunological and structural characterization, and partial amino acid sequences. | we report the first complete purifications of the cytosolic and plastid isozymes of triose phosphate isomerase (tpi; ec 5.3.1.1) from higher plants including spinach (spinacia oleracea), lettuce (lactuca sativa), and celery (apium graveolens). both isozymes are composed of two isosubunits with approximate molecular weight of 27,000; in spinach and lettuce the plastid isozyme is 200 to 400 larger than the cytosolic isozyme. the two isozymes, purified from lettuce, had closely similar amino acid c ... | 1984 | 16663420 |
| magnetic resonance imaging of biological specimens by electron paramagnetic resonance of nitroxide spin labels. | electron paramagnetic resonance imaging was demonstrated on two plant species, apium graveolens and coleus blumei. this was accomplished by soaking stems of these plants in the paramagnetic nitroxide imaging agent 4-hydroxy-2,2,6,6-tetramethylpiperidine-1-oxyl. the experiments were accomplished at l-band frequency (1.4 to 1.9 gigahertz) with single-turn, flat-loop surface coils. one-dimensional imaging spectra were diagnostic of capillary structure and long-term stability. | 1985 | 2981437 |
| in vitro glucan synthesis by membranes of celery petioles: the role of the membrane in determining the type of linkage formed. | glucan synthesis was achieved with an in vitro membrane fraction from the petioles of celery (apium graveolens). the optimum conditions for maximum synthesis were established. the km and vmax for the enzymic system were 1.0 mm and 0.19 microm min-1 mg protein-1, respectively. mechanical damage to the membrane fraction altered the proportion of beta-(1----3) to beta-(1----4) glucosyl linkages that were synthesized. we suggest that cellulose synthesis (beta-(1----4)-linked glucan chains) is contro ... | 1985 | 2936754 |
| the essential oil of apium graveolens var. secalinum and its cercaricidal activity. | the composition of the essential oil of the fresh aerial parts of apium graveolens var. secalinum at its flowering stage, obtained from three different locations in egypt, was investigated. the identification of the components of this oil was carried out by means of analytical gc and gc-ms. the main components in the oil are: alpha- and beta-pinene, myrcene, limonene, cis-beta-ocimene, gamma-terpinene, cis-allo-ocimene, trans-farnesene, humulene, apiol, beta-selinene, senkyunolide and neocnidili ... | 1985 | 4080514 |
| composition of the volatile oils from the roots, leaves and fruits of different taxa of apium graveolens. | 1986 | 17345477 | |
| developmental changes in photosynthetic gas exchange in the polyol-synthesizing species, apium graveolens l. (celery). | developmental changes in photosynthetic gas exchange were investigated in the mannitol synthesizing plant celery (apium graveolens l. ;giant pascal'). greenhouse-grown plants had unusually high photosynthetic rates for a c(3) plant, but consistent with field productivity data reported elsewhere for this plant. in most respects, celery exhibited typical c(3) photosynthetic characteristics; light saturation occurred at 600 micromoles photons per square meter per second, with a broad temperature op ... | 1986 | 16665012 |
| facilitated transport of glucose in isolated phloem segments of celery. | in isolated phloem segments of celery (apium graveolens l.), a tissue highly specific for sucrose and mannitol uptake, glucose uptake occurs at very low rates and exhibits biphasic kinetics. nonpenetrating inhibitors such as parachloromercuribenzene sulfonic acid did not inhibit glucose uptake. however, uptake was greatly inhibited by penetrating inhibitors such as n-ethylmaleimide and carbonylcyanide-m-chlorophenyl hydrazone. carbonylcyanide-m-chlorophenyl hydrazone inhibition of uptake was rev ... | 1987 | 16665765 |
| sucrose uptake in isolated phloem of celery is a single saturable transport system. | the uptake of different sugars was studied in segments of isolated phloem from petioles of celery (apium graveolens l.) in order to determine the kinetics and specificity of phloem loading in this highly uniform conductive tissue. the uptake kinetics of sucrose and the sugar alcohol, mannitol, which are both phloem-translocated, indicated presence of a single saturable system, while uptake of non-phloem sugars (glucose and 3-o-methylglucose) exhibited biphasic kinetics with lower uptake rates th ... | 1987 | 24225708 |
| interaction of cell turgor and hormones on sucrose uptake in isolated phloem of celery. | phloem tissue isolated from celery (apium graveolens l.) was used to investigate the regulation of sucrose uptake by turgor (manipulated by 50-400 milliosomolal solutions of polyethylene glycol) and hormones indoleacetic acid (iaa) and gibberillic acid (ga(3)). sucrose uptake was enhanced under low cellular turgor (increase in the v(max)). furthermore, enhancement of sucrose uptake was due to a net increase in influx rates since sucrose efflux was not affected by cell turgor. manipulations of ce ... | 1987 | 16665557 |
| inheritance of annual habit in celery: cosegregation with isozyme and anthocyanin markers. | vernalization response was determined in an annual and two biennial celery strains, apium graveolens l. and their f2 hybrids. although the annual strain did not require vernalization to bolt, plants exposed to 10°c for 7 days bolted 2 weeks earlier than non-treated plants. inheritance studies based on f2 and backcross segregations demonstrate that annual habit in celery is partially dominant over biennial and determined by a single gene designated hb. cosegregation studies of this trait with nin ... | 1987 | 24241565 |
| use of stem proteins and isozymes for the identification of celery varieties. | separation of stem proteins by sodium dodecyl sulfate polyacrylamide gel electrophoresis permitted classification of 17 celery varieties (apium graveolens l.) into 7 groups. several of these groups could be further subdivided into 11 subgroups with gel electrophoresis patterns of 4 isozyme systems. classification from gel banding patterns was consistent with that based on pedigree histories. | 1987 | 24248490 |
| cis- and trans-neocnidilide; 1h- and 13c-nmr data of some phthalides. | the (1)h- and (13)c-nmr spectra of several naturally occurring phthalides are reported; among others, the new natural compound 3,3alpha- cis-neocnidilide isolated from apium graveolens l. (and also found to be present in anethum graveolens l.). for some compounds, data are compared with previously published data. | 1987 | 17268969 |
| a vesiculo-bullous disease in pigs resembling foot and mouth disease. i. field cases. | eleven incidents of bullae and vesicles on the snouts and less frequently the feet of white-skinned pigs on seven farms are described. bullous and vesicular lesions up to 5 cm in diameter and containing clotted gelatinous fluid were located on the dorsal aspect of the snout, behind the flange. lesions ruptured, became ulcerated, developed scabs and healed within three weeks. there was no transmission to other pigs or ruminants. the condition was associated with contact with green vegetable mater ... | 1987 | 16031362 |
| a vesiculo-bullous disease in pigs resembling foot and mouth disease. ii. experimental reproduction of the lesion. | vesiculo-bullous dermatitis of pigs characterised by presence of vesicles and bullae on the snout and feet of white skinned pigs was reproduced experimentally. leaves of parsnips (pastinaca sativa), or celery (apium graveolens) infected with the fungus sclerotinia sclerotiorum were fed or rubbed on the snouts and feet of white skinned pigs. pigs were then exposed to sunlight or to uv light of intensity approximately 212 m w/m2 at a wavelength 340-360 nm for eight hours per day until vesicles dev ... | 1987 | 16031363 |
| a study of allergens in celery with cross-sensitivity to mugwort and birch pollens. | sixty-one sera with positive rast to mugwort pollen (artemisiae vulgaris) were submitted to rasts for birch pollen (betula verrucosa) and celery (apium graveolens). in 36 cases rast results were positive for celery. in addition, 23 sera presented specific ige to birch pollen. the binding of specific ige to individual allergens in celery, mugwort pollen and birch pollen was studied by the immunoblotting technique. this involved electrophoretic separation of allergenic extracts, electrotransfer of ... | 1988 | 3233726 |
| biosynthesis of sucrose and mannitol as a function of leaf age in celery (apium graveolens l.). | in celery (apium graveolens l.), the two major translocated carbohydrates are sucrose and the acyclic polyol mannitol. their metabolism, however, is different and their specific functions are uncertain. to compare their roles in carbon partitioning and sink-source transitions, developmental changes in (14)co(2) labeling, pool sizes, and key enzyme activities in leaf tissues were examined. the proportion of label in mannitol increased dramatically with leaf maturation whereas that in sucrose rema ... | 1988 | 16665852 |
| anti-inflammatory activity of some iraqi plants using intact rats. | five plants (myrtus communis, apium graveolens, matricaria chamomilla, withania somnifera and achillea santolina) grown in iraq were assessed for their anti-inflammatory activity on intact rats by measuring the suppression of carrageenan-induced paw edema produced by 1/10 of the intraperitoneal ld50 doses for the respective 80% ethanol extracts. acetylsalicylic acid was used as the standard drug. results showed that the plants possessed varying degrees of anti-inflammatory activity and were clas ... | 1989 | 2601356 |
| electrofusion of protoplasts from celery (apium graveolens l.) with protoplasts from the filamentous fungus aspergillus nidulans. | a method was developed for electrofusion of higher-plant protoplasts from celery and protoplasts from the filamentous fungus aspergillus nidulans. initially, methods for the fusion of protoplasts from ecch species were determined individually and, subsequently, electrical parameters for fusion between the species were determined. pronase-e treatment and the presence of calcium ions markedly increased celery protoplast stability under the electrical conditions required and increased fusion freque ... | 1989 | 24212750 |
| somaclonal variant uc-t3: the expression of fusarium wilt resistance in progeny arrays of celery, apium graveolens l. | first generation (s1) progeny, second generation (s2) progeny, and backcross (bc) progeny of a celery (apium graveolens l. var. dulce) somaclonal variant, uc-t3, were evaluated for resistance to the fungus fusarium oxysporum f. sp. apii, race 2 (foa2). chisquare analysis of s1 progeny showed that the expression of resistance did not fit a single-locus model. s2 progeny means were similar among families, except in a heavily infested field. the lowest ranking s2 family in both the lightly infested ... | 1990 | 24220975 |
| severe phototoxic burn following celery ingestion. | a 65-year-old woman developed a severe, generalized phototoxic reaction following a visit to a suntan parlor. history taking revealed that she had consumed a large quantity of celery root (apium graveolens) 1 hour earlier. with the use of thin-layer chromatography, methoxsalen (8-methoxypsoralen) and 5-methoxypsoralen were identified in the extract from a similar celery root. the biologic activity of this extract, as evaluated with the semiquantitative candida albicans inhibition technique, indi ... | 1990 | 2221939 |
| effects of bekh-k-karafs (apium graveolens linn) root on central nervous systme. | bekh-e-karafs has been reported to be effective in a number of ailments in unani system of medicine. the aqueous extract of the drug was screened for c.n.s. depressant, anti-convulsant and analgesic activities. the central depressant and analgesic activities of the drug were found to be significant while anti-convulsant activity was found to be insignificant. the results are reported here. | 1990 | 22556517 |
| [(14) c]-assimilate translocation in the light and dark in celery (apium graveokns) leaves of different ages. | the 2 major photosynthetic products and translocated carbohydrates in celery (apium graveolens l.) are sucrose and the sugar alcohol, mannitol. sucrose is produced and utilized in leaves of all ages. mannitol, however, is synthesized primarily in mature leaves, utilized in young leaves and stored in all leaves. here we show that mannitol export was lower from young, expanding leaves than from older leaves. after a 10 min pulse of (14) co(2) and a 2 h chase in the light or dark there was more rad ... | 1990 | 21087275 |
| host plant resistance and linear furanocoumarin content of apium accessions. | linear furanocoumarin contents and antibiotic resistance to liriomyza trifolii (burgess) were documented for apium species being investigated in a celery breeding program. in no-choice tests, l. trifolii fed more, produced more offspring, and had the highest pupal and adult productivity on the widely planted cultivar 'tall utah' 52-70r (apium graveolens l.). antibiotic effects of the commercial cultivar 'tall utah' 52-70 hk and university of california families 87a-147 and 87a-338, derived from ... | 1990 | 2345223 |
| impact of atmospheric pollution on linear furanocoumarin content in celery. | in a series of laboratory studies, a single 4-hr acidic fog at ph levels associated with commercial celery (apium graveolens l.) production near major population centers in california was found to stimulate development of psoralen, bergapten, xanthotoxin, and isopimpinellin within 24 hr and for at least 120 hr after exposure. at 120 hr posttreatment, the concentrations of phototoxin furanocoumarins (psoralen + bergapten + xanthotoxin) increased 540% in the leaves (to 135 μg/g fresh weight) and 4 ... | 1990 | 24263501 |
| direct observation of cell wall structure in living plant tissues by solid-state c nmr spectroscopy. | solid-state (13)c nuclear magnetic resonance (nmr) spectra of the following intact plant tissues were recorded by the crosspolarization magic-angle spinning technique: celery (apium graveolens l.) collenchyma; carob bean (ceratonia siliqua l.), fenugreek (trigonella foenum-graecum l.), and nasturtium (tropaeolum majus l.) endosperm; and lupin (lupinus polyphyllus lindl.) seed cotyledons. all these tissues had thickened cell walls which allowed them to withstand the centrifugal forces of magic an ... | 1990 | 16667266 |
| xylulose 1,5-bisphosphate synthesized by ribulose 1,5-bisphosphate carboxylase/oxygenase during catalysis binds to decarbamylated enzyme. | xylulose 1,5-bisphosphate (xubp) is synthesized from ribulose 1,5-bisphosphate (rubp) at carbamylated catalytic sites on ribulose 1,5-bisphosphate carboxylase (rubisco) with significant amounts of xubp being formed at ph less than 8.0. xubp has been separated by high performance liquid chromatography and identified by pulsed amperometry from compounds bound to rubisco during catalysis with the purified enzyme and from celery (apium graveolens var utah) leaf extracts. xubp does not bind tightly t ... | 1991 | 16668555 |
| a simple method for obtaining cross-term-free images for diffusion anisotropy studies in nmr microimaging. | the geometric average of two spin-echo images obtained with opposite polarity diffusion gradients yields cross-term-free images that can be directly compared for diffusion anisotropy. this approach is demonstrated here for free water isotropic diffusion and anisotropic diffusion of water in the phloem system of celery (apium graveolens). | 1991 | 1943671 |
| vasodilatory action mechanisms of apigenin isolated from apium graveolens in rat thoracic aorta. | the effect of apigenin, isolated from apium graveolens, on the contraction of rat thoracic aorta was studied. apigenin inhibited the contraction of aortic rings caused by cumulative concentrations of calcium (0.03-3 mm) in high potassium (60 mm) medium, with an ic50 of about 48 microm. after pretreatment it also inhibited norepinephrine (ne, 3 microm)-induced phasic and tonic contraction in a concentration (35-140 microm)-dependent manner with an ic50 of 63 microm. at the plateau of ne-induced t ... | 1991 | 1659912 |
| [the production of furocumarin in apium graveolens l. and pastinaca sativa l. after infection with sclerotinia slcerotiorum]. | roots of celery (apium graveolens) and parsnip (pastinaca sativa) were inoculated with sclerotinia sclerotiorum. at the beginning of the infection it is recognized that the level of furocoumarins rises but decreasing in the further time. trimethylpsoralene was detected neither dc nor hplc. sclerotinia grows poorly in parsnip. in this the high level of furocoumarins prevents the growth of the fungus because the furocoumarins are fungistatic. obvious sclerotinia is able to stimulate the origin of ... | 1991 | 1798717 |
| penetration of celery and alfalfa roots by pratylenchus penetrans as affected by temperature. | a greater percentage of females than juveniles or males of p. penetrans penetrated celery roots grown in infested soil at 5, 18, or 30 c; the difference was greatest at 5 c. the time of initial penetration of alfalfa seedlings inoculated with single nematodes on water agar varied with temperature. females penetrated the seedlings earlier and over a wider range of temperatures than did males or juveniles. the rate of penetration was highest for females. after initial penetration, the penetration ... | 1991 | 19283112 |
| morphological observations of pratylenchus penetrans from celery and strawberry in southern ontario. | pratylenchus penetrans was obtained from premier strawberry in norfolk county and the niagara peninsula and from celery in the latter area. host affected the dimensions of p. penetrans to a greater extent than geographical area in ontario. adults of p. penetrans from southern ontario tended to be smaller than those reported elsewhere. the presence of three lip annules was consistent in the seven populations studied, although in some specimens one of the annules did not entirely encompass the hea ... | 1991 | 19283114 |
| partial purification and characterization of mannitol: mannose 1-oxidoreductase from celeriac (apium graveolens var. rapaceum) roots. | a mannitol:mannose 1-oxidoreductase was isolated from celeriac (apium graveolens var. rapaceum) root tips by fractionation with (nh4)2so4, followed by chromatography on a fractogel deae column and then concentration with (nh4)2so4. this newly discovered mannitol dehydrogenase catalyzes the nad-dependent oxidation of mannitol to mannose, not mannitol to fructose. the sugar product of the enzyme reaction was identified by three independent hplc systems and by an enzymatically linked system as bein ... | 1992 | 1416989 |
| control of thickness of collenchyma cell walls by pectins. | near-isotropic stresses were generated within collenchyma cell walls of celery (apium graveolens l.) by exchanging k(+) for ca(2+) ions, varying the ionic strength and de-esterifying the pectic carboxyl groups, treatments that changed the free-charge density of the pectic polysaccharides. the collenchyma strands swelled radially with increasing free-charge density but there was very little longitudinal swelling. depolymerising the pectins by β-elimination also induced much more radial than longi ... | 1992 | 24178046 |
| induction of somatic embryogenesis using side chain and ring modified forms of phenoxy acid growth regulators. | the induction of somatic embryo development in cell cultures of alfalfa (medicago sativa), celery (apium graveolens), and lettuce (lactuca sativa) was compared for 2,4-dichlorophenoxy-acetic acid (2,4-d) and various phenoxy acid growth regulators. tests using a series of straight chain extensions to the phenoxy acid side chain indicate that phenoxybutanoic acid is active, whereas the phenoxypropanoic and phenoxypentanoic analogs are inactive for the induction of alfalfa embryogenesis. side branc ... | 1992 | 16668836 |
| mannitol synthesis in higher plants : evidence for the role and characterization of a nadph-dependent mannose 6-phosphate reductase. | mannitol is a major photosynthetic product in many algae and higher plants. photosynthetic pulse and pulse-chase (14)c-radiolabeling studies with the mannitol-synthesizing species, celery (apium graveolens l.) and privet (ligustrum vulgare l.), showed that mannose 6-phosphate (m6p) and mannitol 1-phosphate were among the early photosynthetic products. a nadph-dependent m6p reductase was detected in these species (representing two different higher plant families), and the enzyme was purified to a ... | 1992 | 16668806 |
| effect of different carbon sources on relative growth rate, internal carbohydrates, and mannitol 1-oxidoreductase activity in celery suspension cultures. | little information exists concerning the biochemical route of mannitol catabolism in higher plant cells. in this study, the role of a recently discovered mannitol 1-oxidoreductase (mdh) in mannitol catabolism was investigated. suspension cultures of celery (apium graveolens l. var dulce [mill.] pers.) were successfully grown on nutrient media with either mannitol, mannose, or sucrose as the sole carbon source. cell cultures grown on any of the three carbon sources did not differ in relative grow ... | 1993 | 12231996 |
| the alpha-d-glucosyl c-2 hydroxyl is required for binding to the h(+)-sucrose transporter in phloem. | the specificity of uptake of sucrose into isolated phloem tissue from apium graveolens has been investigated using a number of analogues of sucrose. the presence of a single saturable transport system for sucrose was confirmed using the double isotope method of inui and christensen (j. gen. physiol. 50 (1966) 203-224). 4-hydroxyphenyl beta-d-fructofuranoside showed no inhibition of sucrose uptake, whereas 4-hydroxyphenyl alpha-d-glucopyranoside showed competitive inhibition with a ki of 6.7 mm. ... | 1993 | 8399306 |
| tissue printing as a tool for observing immunological and protein profiles in young and mature celery petioles. | tissue printing onto membranes such as nitrocellulose is a technique employed to study the localization of proteins, nucleic acids, and soluble metabolites from freshly cut tissue slices. we probed tissue prints of young and mature celery (apium graveolens) petioles with antibodies raised against two proteins, spinach ribulose-1,5-bisphosphate carboxylase and tomato fruit catalase. the purposes of this study were to determine if these proteins are developmentally regulated and to determine if th ... | 1993 | 12231884 |
| mannose-6-phosphate reductase, a key enzyme in photoassimilate partitioning, is abundant and located in the cytosol of photosynthetically active cells of celery (apium graveolens l.) source leaves. | mannitol, a major photosynthetic product and transport carbohydrate in many plants, accounts for approximately 50% of the carbon fixed by celery (apium graveolens l.) leaves. previous subfractionation studies of celery leaves indicated that the enzymes for mannitol synthesis were located in the cytosol, but these data are inconsistent with that published for the sites of sugar alcohol synthesis in other families and taxa, including apple (malus) and a brown alga (fucus). using antibodies to a ke ... | 1993 | 12231825 |
| studies on ctp:cholinephosphate cytidylyltransferase in apium graveolens. | 1993 | 8395428 | |
| identification and classification of celery cultivars with rapd markers. | twenty-one celery (apium graveolens l. var. dulce) cultivars, one celeriac (var. rapaceum) and one annual smallage (var. secalinum) cultivar were screened for polymorphic rapd (random amplified polymorphic dna) markers with 28 arbitrary 10-mer primers. among a total of 309 bands observed, 29 (9.3%) were polymorphic in the 23 cultivars screened, but only 19 (6.1%) markers were polymorphic within the 21 type dulce cultivars. these markers were sufficient to distinguish each of the cultivars used. ... | 1993 | 24193461 |
| development of genetic markers in celery based on restriction fragment length polymorphisms. | linkage relationships are reported for 34 markers in celery (apium graveolens l. var 'dulce') including 21 rflp, 11 isozyme, and 2 morphological traits. the mapping was carried out in a cross between celery and an annual accession from thailand, a143, and based on f2 segregation of 136 plants. a total of 318 centimorgans (cm) are covered by the markers distributed in 8 linkage groups. probes for the identification of rflps were isolated from a celery cdna library and were also obtained from hete ... | 1993 | 24196065 |
| [plasma levels of psoralens after celery ingestion]. | psoralens are photosensitizing substances present in many vegetables, some of which are routinely consumed. these vegetables are responsible for contact phytophotodermatitis, but it was agreed that they did not produce photodermatitis when taken orally. ljunggren has recently questioned this concept by reporting a case of phototoxic accident which occurred after ingestion of 450 grams of celery roots (apium graveolens). in a study in healthy volunteers we looked for psoralens in blood and analyz ... | 1993 | 8161113 |
| chemoprevention of benzo[a]pyrene-induced forestomach cancer in mice by natural phthalides from celery seed oil. | bioassay-directed fractionation of celery seed oil from the plant apium graveolens (umbelliferae) led to the isolation of five natural products, including d-limonene, p-mentha-2,8-dien-1-ol, p-mentha-8(9)-en-1,2-diol, 3-n-butyl phthalide, and sedanolide. of these compounds p-mentha-2,8-dien-1-ol,3-n-butyl phthalide, and sedanolide exhibited high activities to induce the detoxifying enzyme glutathione s-transferase (gst) in the target tissues of female a/j mice. 3-n-butyl phthalide and sedanolide ... | 1993 | 8446516 |
| effects of sterol biosynthesis inhibitors on phosphatidylcholine biosynthesis in apium graveolens. | 1994 | 7698450 | |
| mannitol metabolism in celery stressed by excess macronutrients. | the effect of excess macronutrients in the root environment on mannitol and sucrose metabolism was investigated in celery (apium graveolens l. var dulce [mill.] pers.). plant growth was inhibited progressively as macronutrient concentration in the media, as measured by electrical conductivity (e.c.), increased from 1.0 to 11.9 decisiemens m-1. plants grown for 35 d at higher e.c. had a lower water content but similar dry weight in their roots, leaves, and petioles compared to plants grown at low ... | 1994 | 12232345 |
| gas exchange and carbon partitioning in the leaves of celery (apium graveolens l.) at various levels of root zone salinity. | both mannitol and sucrose (suc) are primary photosynthetic products in celery (apium graveolens l.). in other biological systems mannitol has been shown to serve as a compatible solute or osmoprotectant involved in stress tolerance. although mannitol, like suc, is translocated and serves as a reserve carbohydrate in celery, its role in stress tolerance has yet to be resolved. mature celery plants exposed to low (25 mm nacl), intermediate (100 mm nacl), and high (300 mm nacl) salinities displayed ... | 1994 | 12232328 |
| phthalide-based host-plant resistance tospodoptera exigua andtrichoplusia ni inapium graveolens. | a chemical basis for the difference in suitability between two celeriac (apium graveolens var.rapaceum) cultivars for the survival and growth ofspodoptera exigua (hübner) andtrichoplusia ni (hübner) was identified as sedanenolide (3-n-butyl-4,5-dihydro-isobenzofuranone). sedanenolide was isolated using a bioassay-driven extraction and purification procedure and was identified using several spectrometric methods. foliar concentrations of sedanenolide were negatively correlated with larval perform ... | 1994 | 24242122 |
| sequence analysis of a mannitol dehydrogenase cdna from plants reveals a function for the pathogenesis-related protein eli3. | mannitol is the most abundant sugar alcohol in nature, occurring in bacteria, fungi, lichens, and many species of vascular plants. celery (apium graveolens l.), a plant that forms mannitol photosynthetically, has high photosynthetic rates thought to results from intrinsic differences in the biosynthesis of hexitols vs. sugars. celery also exhibits high salt tolerance due to the function of mannitol as an osmoprotectant. a mannitol catabolic enzyme that oxidizes mannitol to mannose (mannitol dehy ... | 1995 | 7638158 |
| purification of nad-dependent mannitol dehydrogenase from celery suspension cultures. | mannitol dehydrogenase, a mannitol:mannose 1-oxidoreductase, constitutes the first enzymatic step in the catabolism of mannitol in nonphotosynthetic tissues of celery (apium graveolens l.). endogenous regulation on the enzyme activity in response to environmental cues is critical in modulating tissue concentration of mannitol, which, importantly, contribute to stress tolerance of celery. the enzyme was purified to homogeneity from celery suspension cultures grown on d-mannitol as the carbon sour ... | 1995 | 7630943 |
| effect of cations on the elution of pectic polysaccharides from anion-exchange resins. | conditions for quantitatively eluting six plant pectic polysaccharides from diethylaminoethyl (deae)-columns were established. cations, surprisingly, greatly affected the elution of pectic polysaccharides from these anion-exchange columns. quantitative elution of apple pectic acid was achieved when columns of deae-sephadex a-25 and deae-trisacryl plus-m were developed with 0.5 m nh4cl, 0.5 m licl, or 0.5 m cscl in buffer. in contrast, up to 1 m nacl and kcl in buffer only eluted 6.3 to 54% of th ... | 1995 | 8572266 |
| construction of a genetic linkage map in celery using dna-based markers. | a f2 population of two celery cultivated types (apium graveolens l. var. rapaceum and a. graveolens l. var. secalinum) was used to construct a linkage map consisting of 29 rflp (restriction fragment length polymorphism), 100 rapd (random amplified polymorphic dna), four isozyme, one disease resistance, and one growth habit markers. the map contains 11 major groups and 9 small groups and has a total length of 803 cm with an average distance of 6.4 cm between two adjacent loci. ten percent of the ... | 1995 | 18470150 |
| effects of seed cryopreservation and priming on germination in several cultivars of apium graveolens. | seed germination of seven celery cultivars was studied after storage in liquid nitrogen for 1 or 30 d. cryopreservation was also carried out on pelleted and primed seeds. none of the treatments applied reduced germination percentages. t(50) (time for germination to reach 50%) significantly decreased in florida, utah and istar cultivars when priming, alone or in combination with cryopreservation, was used. | 1995 | 21247906 |
| hepatoprotective activity of apium graveolens and hygrophila auriculata against paracetamol and thioacetamide intoxication in rats. | seeds of apium graveolens l. (apiaceae) and hygrophila auriculata (k. schum.) heine (syn. astercantha auriculata nees, acanthaceae) are used in indian systems of medicine for the treatment of liver ailments. the antihepatotoxic effect of methanolic extracts of the seeds of these two plants was studied on rat liver damage induced by a single dose of paracetamol (3 g/kg p.o.) or thioacetamide (100 mg/kg, s.c.) by monitoring several liver function tests, viz. serum transaminases (sgot and sgpt), al ... | 1995 | 8824736 |
| molecular characterization of api g 1, the major allergen of celery (apium graveolens), and its immunological and structural relationships to a group of 17-kda tree pollen allergens. | individuals suffering from immediate hypersensitivity (type-i allergy) to a particular pollen frequently display intolerance to several foods of plant origin. in this respect, individuals sensitized to birch pollen and/or mugwort pollen frequently display type-i allergic symptoms after ingestion of celery. in this study, we expressed the major allergenic protein of celery, api g 1, which is responsible for the birch-celery syndrome, in the form of a non-fusion protein. the open reading frame of ... | 1995 | 7588792 |
| effects of aqueous celery (apium graveolens) extract on lipid parameters of rats fed a high fat diet. | the antihyperlipidemic property of aqueous celery extract was studied in rats. two groups of wistar rats were fed a high fat diet for eight weeks to induce hyperlipidemia. one group was supplemented with aqueous celery extract in the diet while the other group served as control. at the end of the experiment, a significant reduction was found in the serum total cholesterol (tc), low density lipoprotein cholesterol (ldl-c), and triglyceride (tg) concentrations in the celery-treated rats. however, ... | 1995 | 7700983 |
| immunolocalization of mannitol dehydrogenase in celery plants and cells. | immunolocalization of mannitol dehydrogenase (mtd) in celery (apium graveolens l.) suspension cells and plants showed that mtd is a cytoplasmic enzyme. mtd was found in the meristems of celery root apices, in young expanding leaves, in the vascular cambium, and in the phloem, including sieve-element/companion cell complexes, parenchyma, and in the exuding phloem sap of cut petioles. suspension cells that were grown in medium with mannitol as the sole carbon source showed a high anti-mtd cross-re ... | 1996 | 8938403 |
| molecular cloning of mannose-6-phosphate reductase and its developmental expression in celery. | compared with other primary photosynthetic products (e.g. sucrose and starch), little is known about sugar alcohol metabolism, its regulation, and the manner in which it is integrated with other pathways. mannose-6-phosphate reductase (m6pr) is a key enzyme that is involved in mannitol biosynthesis in celery (apium graveolens l.). the m6pr gene was cloned from a leaf cdna library, and clonal authenticity was established by assays of m6pr activity, western blots, and comparisons of the deduced am ... | 1997 | 9112783 |
| estimation of polymer rigidity in cell walls of growing and nongrowing celery collenchyma by solid-state nuclear magnetic resonance in vivo. | when the growth of a plant cell ceases, its walls become more rigid and lose the capacity to extend. nuclear magnetic resonance relaxation methods were used to determine the molecular mobility of cell wall polymers in growing and nongrowing live celery (apium graveolens l.) collenchyma. to our knowledge, this is the first time this approach has been used in vivo. decreased polymer mobility in nongrowing cell walls was detected through the 13c-nuclear magnetic resonance spectrum by decreases in t ... | 1997 | 12223826 |
| subcellular localization of celery mannitol dehydrogenase. a cytosolic metabolic enzyme in nuclei. | mannitol dehydrogenase (mtd) is the first enzyme in mannitol catabolism in celery (apium graveolens l. var dulce [mill] pers. cv florida 638). mannitol is an important photoassimilate, as well as providing plants with resistance to salt and osmotic stress. previous work has shown that expression of the celery mtd gene is regulated by many factors, such as hexose sugars, salt and osmotic stress, and salicylic acid. furthermore, mtd is present in cells of sink organs, phloem cells, and mannitol-gr ... | 1997 | 9414553 |
| sugar repression of mannitol dehydrogenase activity in celery cells. | we present evidence that the activity of the mannitol-catabolizing enzyme mannitol dehydrogenase (mtd) is repressed by sugars in cultured celery (apium graveolens l.) cells. furthermore, this sugar repression appears to be mediated by hexokinases (hks) in a manner comparable to the reported sugar repression of photosynthetic genes. glucose (glc)-grown cell cultures expressed little mtd activity during active growth, but underwent a marked increase in mtd activity, protein, and rna upon glc starv ... | 1997 | 12223706 |
| isolation and characterization of soluble boron complexes in higher plants. the mechanism of phloem mobility of boron. | boron (b) polyol complexes have been isolated and characterized from the phloem sap of celery (apium graveolens l.) and the extrafloral nectar of peach (prunus persica l.). in celery the direct analysis of untreated phloem sap by matrix-assisted laser desorption-fourier transform mass spectrometry, with verification by high-performance liquid chromagraphy and gas chromatography-mass spectrometry, revealed that b is present in the phloem as the mannitol-b-mannitol complex. molecular modeling furt ... | 1997 | 9046600 |
| assessment of the transfer of 137cs in three types of vegetables consumed in hong kong. | a dynamic food chain model has been built for the modeling of the transfer of 137cs in three types of vegetables consumed in hong kong, namely, white flowering cabbage (brassica chinensis), head lettuce (lactuca sativa) and celery (apium graveolens). some parameters have been estimated from the experimental data obtained in this work. the experimental data include the transfer factors of 137cs from soil to the different vegetable species which are determined through high resolution gamma spectro ... | 1998 | 9745700 |
| anti-nociceptive and anti-inflammatory effects of some jordanian medicinal plant extracts. | the anti-nociceptive effect of ethanolic extract of 11 traditionally used jordanian plants was studied by using the acetic acid-induced writhing and hot-plate test in mice. the anti-inflammatory effect of these plants was determined by xylene-induced ear oedema in mice and cotton pellet granuloma test in rats. mentha piperita, cinnamomum zeylanicum, apium graveolens, eucalyptus camaldulentis, and ruta graveolens possess an anti-nociceptive effect against both acetic acid-induced writhing and hot ... | 1998 | 9582001 |
| modulation of phosphatidylcholine biosynthesis in celery by exogenous fatty acids. | the effects of c16 and c18 fatty acids on the synthesis of phosphatidylcholine were studied in apium graveolens cell suspension cultures and postmitochondrial supernatants. when cells were exposed to exogenous oleic acid, the rate of phosphatidylcholine biosynthesis increased 1.4-fold within 5 min of the addition of the fatty acid to the culture medium. the sensitivity of microsomal ctp:cholinephosphate cytidylyltransferase (ec 2.7.7.15) to saturated and unsaturated fatty acids was monitored thr ... | 1999 | 9891932 |
| assembly and disassembly of the peripheral architecture of the plant cell nucleus during mitosis | the architecture of the nuclei of higher plants includes a structure similar to the nuclear lamina of vertebrates. changes in this structure were monitored during mitosis in carrot (daucus carota l.) and celery (apium graveolens l.) cells by immunofluorescence microscopy using an antibody that recognized the nuclear-matrix protein nmcp1. this protein has been shown to be localized exclusively at the periphery of the nucleus (k. masuda et al. 1997, exp cell res 232: 173-187). immunofluorescence w ... | 1999 | 10592045 |
| survey of crop losses in response to phytoparasitic nematodes in the united states for 1994. | previous reports of crop losses to plant-parasitic nematodes have relied on published results of survey data based on certain commodities, including tobacco, peanuts, cotton, and soybean. reports on crop-loss assessment by land-grant universities and many commodity groups generally are no longer available, with the exception of the university of georgia, the beltwide cotton conference, and selected groups concerned with soybean. the society of nematologists extension committee contacted extensio ... | 1999 | 19270925 |
| effect of different drying methods on concentrations of several phytochemicals in herbal preparation of 8 medicinal plants leaves. | the effect of oven drying at 50ᵒc ± 1ᵒc for 9 hour, 70ᵒc ± 1ᵒc for 5 hour and freeze drying on retention of chlorophyll, riboflavin, niacin, ascorbic acid and carotenoids in herbal preparation consisting of 8 medicinal plants was evaluated. the medicinal plants selected were leaves of apium graveolens (saderi), averrhoa bilimbi (belimbing buluh), centella asiatica (pegaga), mentha arvensis (pudina), psidium guajava (jambu batu), sauropus androgynous (cekor manis), solanum nigrum (terung meranti) ... | 1999 | 22692357 |
| celery (apium graveolens l.) parenchyma cell walls examined by atomic force microscopy: effect of dehydration on cellulose microfibrils. | atomic force microscopy (afm) was used to image celery (apium graveolens l.) parenchyma cell walls in situ. cellulose microfibrils could clearly be distinguished in topographic images of the cell wall. the microfibrils of the hydrated walls appeared smaller, more uniformly distributed, and less enmeshed than those of dried peels. in material that was kept hydrated at all times and imaged under water, the microfibril diameter was mainly in the range 6-25 nm. the cellulose microfibril diameters we ... | 2000 | 11219580 |
| nadph supply and mannitol biosynthesis. characterization, cloning, and regulation of the non-reversible glyceraldehyde-3-phosphate dehydrogenase in celery leaves. | mannitol, a sugar alcohol, is a major primary photosynthetic product in celery (apium graveolens l. cv giant pascal). we report here on purification, characterization, and cdna cloning of cytosolic non-reversible glyceraldehyde-3-p dehydrogenase (nr-g3pdh, ec 1.2.1. 9), the apparent key contributor of the nadph required for mannitol biosynthesis in celery leaves. as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, purified nr-g3pdh showed a molecular mass of 53 kd. a 1,73 ... | 2000 | 10982446 |
| the sucrose transporter of celery. identification and expression during salt stress. | in celery (apium graveolens l.), long-distance transport of reduced carbon occurs both in the form of sucrose (suc) and mannitol. the presence of mannitol has been related to the resistance of celery to salt stress. to investigate the transport events occurring during salt stress, we have cloned the h(+)/suc transporter of celery agsut1 (a. graveolens suc uptake transport 1) from a mature leaf cdna library. the function of the encoded protein was confirmed by expression in yeast. agsut1 is a h(+ ... | 2000 | 10759540 |
| cloning of the minor allergen api g 4 profilin from celery (apium graveolens) and its cross-reactivity with birch pollen profilin bet v 2. | profilin is a panallergen that is recognized by ige from about 20% of birch pollen- and plant food-allergic patients. a subgroup of celery-allergic patients shows ige-reactivity with this minor allergen. to investigate the ige-binding potential and cross-reactivity of celery profilin at the molecular level, this study was aimed at the cloning and immunological characterization of this allergen. | 2000 | 10848918 |
| bioactive compounds and 1,3-di[(cis)-9-octadecenoyl]-2-[(cis,cis)-9, 12-octadecadienoyl]glycerol from apium graveolens l. seeds. | bioassay-directed isolation and purification of the hexane extract of apium graveolens l. seeds led to the characterization of three compounds: beta-selinene (1), 3-n-butyl-4,5-dihydrophthalide (2) and 5-allyl-2-methoxyphenol (3). the structures of these compounds were established by using (1)h and (13)c nmr spectral methods. compounds, 1-3 demonstrated 100% mortality on fourth-instar aedes aegyptii larvae at 50, 25, and 200 microg ml(-)(1), respectively, in 24 h. also, 2 inhibited the growth of ... | 2000 | 10995271 |
| identification of a mannitol transporter, agmat1, in celery phloem. | a celery petiole phloem cdna library was constructed and used to identify a cdna that gives saccharomyces cerevisiae cells the ability to grow on mannitol and transport radiolabeled mannitol in a manner consistent with a proton symport mechanism. this cdna was named agmat1 (apium graveolens mannitol transporter 1). the expression profile in source leaves and phloem was in agreement with a role for mannitol in phloem loading in celery. the identification in eukaryotes of a mannitol transporter is ... | 2001 | 11251106 |
| mosquitocidal, nematicidal, and antifungal compounds from apium graveolens l. seeds. | the methanolic extract of apium graveolens seeds was investigated for bioactive compounds and resulted in the isolation and characterization of mosquitocidal, nematicidal, and antifungal compounds sedanolide (1), senkyunolide-n (2), and senkyunolide-j (3). their structures were determined by 1h and 13c nmr spectral methods. compounds 1-3 gave 100% mortality at 25, 100, and 100 microg ml(-1), respectively, on the nematode, panagrellus redivivus. compound 1 showed 100% mortality at 50 microg ml(-1 ... | 2001 | 11305251 |
| alpha-tocopherol content in 62 edible tropical plants. | vitamin e was determined by the high-performance liquid chromatography (hplc) method. all the plants tested showed differences in their alpha-tocopherol content and the differences were significant (p < 0.05). the highest alpha-tocopherol content was in sauropus androgynus leaves (426.8 mg/kg edible portion), followed by citrus hystrix leaves (398.3 mg/kg), calamus scipronum (193.8 mg/kg), starfruit leaves averrhoa belimbi (168.3 mg/kg), red pepper capsicum annum (155.4 mg/kg), local celery apiu ... | 2001 | 11410015 |
| analysis of celery (apium graveolens) mannitol dehydrogenase (mtd) promoter regulation in arabidopsis suggests roles for mtd in key environmental and metabolic responses. | of the growing list of promising genes for plant improvement, some of the most versatile appear to be those involved in sugar alcohol metabolism. mannitol, one of the best characterized sugar alcohols, is a significant photosynthetic product in many higher plants. the roles of mannitol as both a metabolite and an osmoprotectant in celery (apium graveolens) are well documented. however, there is growing evidence that 'metabolites' can also have key roles in other environmental and developmental r ... | 2001 | 11725947 |
| mapping peroxidase in plant tissues by scanning electrochemical microscopy. | scanning electrochemical microscopy has been firstly used to map the enzymatic activity in natural plant tissues. the peroxidase (pod) was maintained in its original state in the celery (apium graveolens l.) tissues and electrochemically visualized under its native environment. ferrocenemethanol (fma) was selected as a mediator to probe the pod in celery tissues based on the fact that pod catalyzed the oxidation of fma by h(2)o(2) to increase fma(+) concentration. two-dimensional reduction curre ... | 2001 | 11694396 |
| the calcium-binding activity of a vacuole-associated, dehydrin-like protein is regulated by phosphorylation. | a vacuole membrane-associated calcium-binding protein with an apparent mass of 45 kd was purified from celery (apium graveolens). this protein, vcab45, is enriched in highly vacuolate tissues and is located within the lumen of vacuoles. antigenically related proteins are present in many dicotyledonous plants. vcab45 contains significant amino acid identity with the dehydrin family signature motif, is antigenically related to dehydrins, and has a variety of biochemical properties similar to dehyd ... | 2002 | 12376635 |